CN103477748A - Method for preventing and controlling soil-borne diseases of vegetables by aid of crop straws - Google Patents
Method for preventing and controlling soil-borne diseases of vegetables by aid of crop straws Download PDFInfo
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Abstract
The invention relates to a method for preventing and controlling soil-borne diseases of vegetables by the aid of crop straws. The method is characterized in that soil is flooded after the crop straws are applied to fields. The method particularly includes steps of thoroughly removing preceding crop residues in field parcels at the soil temperature higher than 20 DEG C; encircling the peripheries of the field parcels by soil to form soil ridges with the heights of about 20 centimeters; uniformly broadcasting the smashed crop straws with the lengths ranging from 10 centimeters to 15 centimeters into the fields; plowing soil by the depth ranging from 18 centimeters to 20 centimeters; flooding the field parcels by irrigation water for 5 days; keeping the depths the water above the surfaces of soil layers of the fields larger than 5 centimeters in flooding periods; performing rotary tillage on the soil by the depth ranging from 18 centimeters to 20 centimeters after the 5 days; flooding the field parcels for 15 days; stopping flooding the field parcels when oxidation reduction potential of the soil of the fields is reduced and ranges from 0mv to 40mv; naturally drying the soil and then applying fertilizers to the soil; performing rotary tillage on the soil and then planting crops in the soil. The applied fertilizers include 30 to 40 kilograms of urea and 20 to 30 kilograms of potassium phosphate fertilizers.
Description
Technical field
The present invention relates to a kind of method of utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable, after specifically agricultural crop straw suitably being pulverized, evenly spread fertilizer over the fields to field, the submerging treatment certain hour will be carried out after Soil tillage, then the water of draining, the vegetables of planting after soil natural dries, can reach the effect of prevention and control soil-borne diseases of vegetable.Belong to and utilize agricultural wastes to carry out the integrated control field to vegetable disease.
Background technology
China is large agricultural country, and agricultural crop straw output is large, distribution is wide, kind is many, is the precious resources of life of farmers and agricultural development for a long time always.In recent years, fast development along with the crop breeding technology, the high-yield crop kind is constantly updated and is added improving constantly of agricultural planting level, grain and yield of commercial crops are soaring year by year, the generation of agricultural crop straw (hereinafter to be referred as " stalk ") is increasing year by year also, within 2010, national stalk theoretical resources amount is 8.4 hundred million tons, can collect stock number and be about 700,000,000 tons.The stalk kind be take paddy rice, wheat, corn etc. as main, wherein, and approximately 2.11 hundred million tons of straw, approximately 1.54 hundred million tons of wheat straws.The stalk main component is cellulose, hemicellulose and lignin, and in environment, natural degradation speed is slow, and the straw decomposition difficulty that degree of lignification is high is larger; In addition, still lack at present economical and technical all feasible, straw collecting and storaging and transport technology that can large-scale promotion, make stalk arbitrarily to abandon, burn phenomenon serious, not only cause the wasting of resources also to bring series of environmental problems.Therefore, accelerate to advance straw utilization, for stablizing the Agro-ecology balance, alleviate resource constraint, alleviate environmental pressure all tool be of great significance.
2012, China take the facility cultivation area that vegetables are main body and has broken through 4,200,000 hm
2, than 139.5 ten thousand hm of 1999 last century Mos
2increased nearly 3 times, growing vegetables has become the leading industry that drives farmer richness in high-efficiency agriculture.But medium-term and long-term " four height " (high cropping index, high temperature, high humidity, high fertile) and the continuous cropping of facility plantation makes soil quality constantly worsen, soil salt accumulation, nutrient imbalance, the microbial population unbalance of structure, pathogen grows in a large number, and soil-borne disease increases the weight of increasingly.In facility cultivation development area early, the annual economic loss caused by soil-borne diseases of vegetable accounts for the 20-30% of vegetables income from sales, soil-borne disease has not only become the bottleneck that the restriction facilities vegetable is produced, and will be that the facility cultivation industry faces and problem demanding prompt solution for a long time.
For the control of soil-borne disease, mainly contain the measures such as chemical pesticide, crop rotation, grafting, biopesticide, soil regulation and control, soilless culture and soil disinfection.To overground part, disease control has effect preferably to chemical pesticide, but the proterties of soil complexity makes chemical pesticide greatly reduce the control efficiency of soil-borne disease, strengthen pesticide dosage and cause again pathogen to develop immunity to drugs when bringing environmental pollution, agricultural product security risk; Crop rotation is a kind of approach that solves preferably soil-borne disease, but consider the regional characteristic of vegetable crop, condition of sales and the economic benefit of agricultural product, vegetables production base in scale, be difficult to adopt the means of rice field-upland field rotation, and also lack effective under the scale condition, economic, technical feasible pattern at present; Grafting is mainly for the soil-borne disease problem, but stock variety is limited, and wastes time and energy, and the stock cost is also higher, and quality of agricultural product significantly is worse than the product of not grafting; Biopesticide has better effects to some soil-borne disease under controlled condition, but the soil colonization ability of biocontrol microorganisms is subject to the Effects of Factors such as environment, region, weather larger, and preventive effect is unstable.Soilless culture is had relatively high expectations to the culture techniques such as cultivation matrix raw material and nutrient management, and in conjunction with China's actual conditions, in the following long term, China remains with soil cultivation and takes pattern as the leading factor.Take soil disinfection as the main soil control technique that simultaneously has reclamation result concurrently be the effective way of prevention and control soil-borne disease, but the mechanism of action difference of different controlled material, effect is also variant, therefore selects targetedly soil controlled material and method most important.
A lot of research and application shows that straw-returning can improve soil organic matter content both at home and abroad, improves soil aggregate, increases the various nutrient contents of soil, General Promotion soil Comprehensive Traits, culture fertility, improve plant recovery of nutrient, promote plant growth, improve output and improve quality of agricultural product.
Decomposition product kind after straw-returning is a lot, and most of decomposition product can be utilized as Nutrient Absorption by crops.What wherein cause large quantity research and concern is mainly organic acid, the phenolic acid class allelochemical that straw decomposition produces, this two classes material promotes plant growth under low concentration, can certain murder by poisoning be arranged to plant under high concentration, affecting plant and grow normally, is also the problem that the straw-returning process wants emphasis to consider and must solve by technological means; But simultaneously,, a large amount of research and practice application both at home and abroad also shows that these allelochemicals have even killing effect of strong growth inhibition to many phytopathogens and weeds, can be for the disease pest and weed of prevention and control various crop.In addition, the generation time of the decomposition speed after straw-returning and allelochemical and generation are subject to the impact of stalk consumption and soil water content larger.Under dry farming, straw decomposition speed is slower than the decomposition speed under the waterflooding state, and therefore, allelochemical discharges that the lasting time is long, concentration is low.We studies show that every mu is gone back 400 kilograms, field wheat stalk, the decomposition vegetables such as capsicum, tomato of planting after 14 days under the waterflooding state, plant has not been had to the allelopathic effect, and, under the conventional moisturizing state of soil, straw decomposition there is obvious allelopathic effect to above-mentioned two kinds of vegetables after 14 days.
Waterflooding is the processing method of the pests such as the sick worm of a kind of ancient elimination soil, germ.Also is no lack of the report that adopts waterflooding prevention and control soil-borne disease of crop both at home and abroad.But much research also shows, waterflooding is difficult to reach the effect of eliminating pathogen fully separately.
Having both at home and abroad manyly has the even bibliographical information of killing effect of growth inhibition about the straw decomposition product to multiple soil-borne pathogen, thinks that the allelochemicals such as organic acid that straw degradative produces, phenolic acid will directly kill or suppress pathogen, other decomposition products of stalk can also provide the abundant nutrition source to the insensitive edaphon of allelochemical (particularly bacterium) for those simultaneously, significantly increase the Quantityanddiversity of edaphon, strengthen biological activity of soil, improve plant recovery of nutrient, promoting plant growth and enhance disease resistance can, but these reports are all under the lab simulation dry farming, adopt stalk to cover or stalk is evenly admixed in soil after certain hour is cultivated in moisturizing, collect straw decomposition liquid in vitro, measure the growth inhibition effect to the target pathogen under the pure culture state, differ larger with actual field soil environment.And pathogen directly is inoculated in the soil that contains stalk, continuing under flooding condition, so far there are no to the inhibitory action of pathogen in soil with to the open report of soil-borne diseases of vegetable control effect for original position research straw decomposition product.
Summary of the invention
The object of the invention is to: on the one hand, large for China's agricultural crop straw generation, lack efficient, economic, the workable production reality of utilizing technology; On the other hand, the high-efficiency agriculture fast development, the modern plant soil-borne disease sharply occurs and increases the weight of year by year, still do not have at present economy, efficiently can spread the present situation of prevention and control measure, a kind of method of utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable is proposed.
The object of the present invention is achieved like this: a kind of method of utilizing agricultural crop straw prevention and control facilities vegetable soil-borne disease, it is characterized in that: after agricultural crop straw is applied to field, soil is carried out to submerging treatment, concrete steps are: soil temperature is more than 20 ℃, by the residual body of preceding crop in field, remove clean, the field surrounding is played to approximately 20 centimetres of high ribs with fortifield village, the agricultural crop straw that is crushed to the 10-15 centimetre length is evenly spread fertilizer over the fields to field, then 18-20 centimetre of soil ploughing is dark, with irrigation water, field is carried out to submerging treatment 5 days, during waterflooding, the field depth of water remains on the soil layer surface more than 5 centimetres, dark by soil rotary tillage 18-20 centimetre after 5 days, continue to keep waterflooding 15 days, when being reduced to 0-40mv, finishes soil redox potential waterflooding, after drying, soil natural applies chemical fertilizer, by the crop of planting after soil rotary tillage, the amount of application of chemical fertilizer is: 30-40 kilogram urea and 20-30 kilogram potassium phosphate fertilizer.
In the present invention, described agricultural crop straw refers to wheat, paddy rice or maize straw, and consumption is every mu of 400-600 kilogram, stalk moisture 12-14%.
The invention has the advantages that: at first, stalk source is abundant and cheap and easy to get, and stalk returns the field as fertilizer sources and uses and alleviate environmental pollution pressure, to improvement soil with the benefit of plant growth is extensively approved; Secondly, waterflooding has obtained permanent checking and abundant affirmation to the improved effect of soil property, after straw-returning, coordinate submerging treatment can accelerate the decomposition speed of stalk in soil, and make the materials such as organic acid and phenolic acid concentrate at short notice a large amount of release, reach the effect of killing or suppressing pathogen.This invention input cost is low, and whole application is simple, and easy operating, for new approach is opened up in the efficient utilization of soil-borne disease control and stalk.
We gather a plurality of growing vegetables base, Jiangsu Province soil-borne disease and serious soil occurs carry out paddy rice stalk waterflooding 20 days and process, result shows, with common waterflooding, compare, aspect physicochemical property, stalk is processed obviously raising of soil organic matter content, soil organic acid, always phenolic content significantly increases, and the electrical conductivity of soil, redox potential and ammonium nitrogen content significantly descend.On biological property, in the stalk submerging treatment, the soil polyphenol oxidase is lived, cellulase activity is significantly higher than contrast, and the stalk waterflooding extremely significantly increases soil bacteria quantity.The pathogen Phytophthora capsici is carried out to the quantitative PCR detection result and show, conventional waterflooding also can reduce Phytophthora capsici quantity, but not remarkable, and the stalk waterflooding significantly reduces Phytophthora capsici quantity.Conventional waterflooding 20 days, the sick incidence of disease of capsicum is 50%, more than 35 days, the incidence of disease is below 10% in waterflooding, and 20 days incidences of disease of rice straw waterflooding are just 0.This technology has not only shortened the waterflooding time but also has obviously improved protection effect, and the improvement of facility cultivation soil property, soil-borne disease prevention and control and the sustainable use of facility soil and the sustainable development of industrialized agriculture are significant.
The present invention's raw material used is cheap and easy to get, and method of operating is simple and easy, and input cost is low, and effect is significantly lasting.
The applicant finds by the research in early stage, carry out again submerging treatment owing to apply the quantity of organic material in soil after, during waterflooding, the organic materials decomposition causes soil generation series of physical chemistry and biological respinse, finally causes the soil environment that is unfavorable for the pathogen growth and breeding.If every mu is used 600 kg of water rice straws, continuing waterflooding 20 days, the elimination factor of Phytophthora capsici germ in soil is reached more than 95%, is 100% to the control effect of capsicum epidemic disease, and common waterflooding is only 60-65% to the elimination factor of pathogen, to the disease control effect, is 65% left and right; Use 400 kilograms of wheat stalks for every mu, the submerging treatment straw decomposition liquid of 14 days is 100% to the inhibiting rate of withered germ of water-melon spore germination, and common waterflooding is 20-30% to the pathogen inhibition of germination.After straw-returning, waterflooding can also significantly increase soil organic matter content, bio-diversity and biologically active, continues to suppress the generation of soil-borne disease, has broad application prospects simultaneously.
The accompanying drawing explanation
Fig. 1 is the impact of rice straw waterflooding on soil redox potential.
Fig. 2 is the impact of rice straw waterflooding on soil organic matter content.
Fig. 3 is the impact of rice straw waterflooding on soil organic acid content.
Fig. 4 is the impact of rice straw waterflooding on the soil phenolic content.
Fig. 5 is the impact of rice straw waterflooding on the capsicum epidemic disease incidence of disease.
Fig. 6 is the rice straw waterflooding in soil and the impact of capsicum rhizosphere Phytophthora capsici quantity.
Fig. 7 is the inhibitory action of wheat stalk decomposition liquid to the withered germ of water-melon conidia germination, and wherein: A figure is the effect of clear water contrast, and B figure is the effect of adding wheat straw decomposition liquid.
The CK related in figure is blank group; F is conventional waterflooding group; FS is stalk waterflooding group.
Embodiment
embodiment 1
the impact of rice straw waterflooding on soil physical and chemical property
Test soil is taken from Huaian Gao Gouzhen capsicum and is produced booth, and rice straw is taken from academy of agricultural sciences, Jiangsu Province paddy field, pathogen be the Phytophthora capsici germ (
phytophthora capsici), can obtain from Chinese Academy of Agricultural Sciences's agricultural microorganism fungus kind preservation center.For the examination capsicum variety, be Lip river green pepper No. 5.The Phytophthora capsici spore liquid is evenly added in test soil, and making the mould spore concentration of soil epidemic disease is the mould spore of 200 epidemic disease/gram dry ground.3 processing are established in test: (1) contrast (CK): after the soil inoculation Phytophthora capsici, keep soil water content at 16-18%, naturally place.(2) conventional waterflooding (flooding, F): carry out submerging treatment after the soil inoculation Phytophthora capsici.(3) stalk rice straw waterflooding (flooding with straw, FS): add again 1%(W/W after the soil inoculation Phytophthora capsici), then carry out submerging treatment.Submerging treatment in conventional waterflooding and rice straw waterflooding is all to carry out in Plastic Drum (end footpath * bore * height=25cm * 34cm * 30cm), every barrel of bright soil of 14kg, and every processing repeats twice.Waterflooding Soil during Period surface keeps the 10cm water layer.Plastic Drum is placed under the environment that room temperature is 28 ℃ of left and right to 14 days submerging treatment time.After waterflooding finishes, drain surface water, then soil is spread out be placed in dark ventilation dry to water content be 20% left and right, measure soil physical and chemical property.
1.1 the impact of rice straw waterflooding on redox potential
The results are shown in accompanying drawing 1.Rice straw waterflooding and conventional submerging treatment descend gradually in the Eh of waterflooding Soil during Period value, and rice straw waterflooding group fall significantly is greater than conventional waterflooding group.When waterflooding finishes, the Eh value of conventional waterflooding group and rice straw waterflooding group be respectively 112 with-116mv, add stalk after waterflooding significantly reduced soil redox potential.Stalk is organic materials, and under the waterflooding situation, decomposition needs to consume oxygen, makes Soil Eh significantly reduce, and accelerates the growth and breeding of the dead of pathogen or inhibition pathogen, reduces the disease occurrence probability.
1.2 the impact of rice straw waterflooding on soil organic matter content
The results are shown in accompanying drawing 2.During waterflooding, rice straw waterflooding group soil organic matter content rises to 2.32% gradually by 1.05%, and control group and the variation of conventional waterflooding group are less.Soil organic matter content is the important indicator of characteristics of soil fertility, and soil carries out the rice straw submerging treatment and is conducive to increase soil organic matter content, improves soil water-retaining, fertilizer conservation and cushioning effect.
1.3 the impact of rice straw waterflooding on soil organic acid and phenolic content
From accompanying drawing 3 and accompanying drawing 4, during waterflooding, control group and conventional waterflooding group organic acid and phenolic content change not significantly, and rice straw waterflooding group organic acid, phenolic content rise rapidly.When waterflooding finishes, the soil organic acid of rice straw waterflooding and phenolic content are respectively 4.41 and 3.73 times of waterflooding group, and existing document shows that these phenolic acid have strong growth inhibitory effect to Phytophthora capsici and other plant soil-borne pathogen.
the impact of embodiment 2 rice straw waterflooding on activity of soil xylanase
Gather the soil after embodiment 1 submerging treatment is dried, measure activity of soil xylanase.
From table 1, the activity of soil xylanase of rice straw waterflooding is all higher than contrast and conventional waterflooding.Soil enzymatic activities is an important indicator of soil biology proterties, and generally, healthy soil has higher soil xylanase and lives.In addition, the main component of phytophthora blight of pepper cell wall is cellulose, and the active raising of soil cellulase can suppress the growth of Phytophthora capsici germ or this germ of degrading, and reduces the capsicum epidemic disease occurrence probability.
The impact of table 1 rice straw waterflooding on activity of soil xylanase
| ? | Contrast | Conventional waterflooding | The rice straw waterflooding |
| Cellulase activity | 0.14±0.01 | 0.16±0.02 | 0.31±0.02 |
Annotate: the unit that soil xylanase is lived is mg glucose/(10g72h).
embodiment 3: the inhibitory action of rice straw waterflooding to Phytophthora capsici growth in soil
The soil that embodiment 1 submerging treatment is crossed adopts quantitative PCR (RT-PCR) method to measure Phytophthora capsici quantity in each processing.The Phytophthora capsici assay method: it is the FastDNA SPIN Kit for Soil that MP company produces that soil DNA extracts used kit, according to operation instruction, extracts soil DNA.Special primer is CAPFW(5'-TTTAGTTGGGGGTCTTGTACC-3') and CAPRV1(5'-CCTCCACAACCAGCAACA-3'), the purpose clip size is about 450bp.The RT-PCR reaction kit adopts the SYBR premix Ex Taq of TaKaRa company.The fluorescent quantitative PCR system is: 2 * SYBR@PreMix Ex Taq (TaKaRa), 10 μ L, 50 * ROX Reference Dye II (TaKaRa), 0.4 μ L, each 0.4 μ L of 20 μ mol/L primers, template 2 μ L, ddH2O 6.8 μ L, amplification system is 20 μ L.Response procedures is: 95 ℃, and 30 s, 1 circulation; 95 ℃ of 5s, 60 ℃ of 34s, 40 circulations.The quantitative fluorescent PCR instrument is 7500 types (Applied Biosystems, USA).To contain 10,40,160,640,2560,6 standard soil samples of 10240 Phytophthora capsici spores/gram dry ground and the soil DNA of Phytophthora capsici concentration sample to be measured carry out the SYBR pcr amplification simultaneously, draw out calibration curve with 7500 software, and calculate the concentration of unknown sample soil Phytophthora capsici.
Bright as can be seen from Table 2, before waterflooding starts, in soil, the initial concentration of Phytophthora capsici spore is 218.1 Phytophthora capsicis/gram dry ground; After waterflooding finishes, the Phytophthora capsici quantity of contrast, conventional waterflooding and rice straw waterflooding is respectively 31.5,6.8 and 2.5 Phytophthora capsicis/gram dry ground, and the rice straw waterflooding has significantly reduced the quantity of Phytophthora capsici in soil.
The impact of table 2 rice straw waterflooding on Phytophthora capsici quantity
| ? | Contrast | Conventional waterflooding | The rice straw waterflooding |
| Phytophthora capsici quantity (individual spore/gram soil) | 31.5 | 6.8 | 2.5 |
embodiment 4 rice straw waterflooding reach the lasting inhibitory action to Phytophthora capsici to the control effect of capsicum epidemic disease
The soil of submerging treatment in embodiment 1 is divided and installs in the plastic basin alms bowl, transplant 4 the leaf phase capsicum, 45 days " Invest, Then Investigate " capsicum incidences of disease.
From accompanying drawing 5: the incidence of disease of contrast, conventional waterflooding and rice straw waterflooding is respectively 100%, 16.67% and 0, illustrate conventional waterflooding can be on certain degree the prevention and control capsicum epidemic disease, and the rice straw waterflooding can suppress the generation of capsicum epidemic disease fully.Visible, the rice straw waterflooding has higher control effect to capsicum epidemic disease.
Accompanying drawing 6 shows, after the capsicum of planting, control group Phytophthora capsici quantity sharply rises, and conventional waterflooding group has certain rising.Capsicum plant after the 45th day the time, contrast and two groups of soil Phytophthora capsici concentration of conventional waterflooding are respectively 339.2 and 35.9 Phytophthora capsicis/gram dry ground, rice straw waterflooding group does not detect Phytophthora capsici.Visible rice straw submerging treatment not only can significantly reduce the quantity of Phytophthora capsici in soil during waterflooding, and after capsicum is planted, still can continue to suppress the growth and breeding of Phytophthora capsici at the capsicum rhizosphere, reaches the effect that continues the prevention and control disease.
Wheat stalk is shredded to 5-10 centimetre, with 0.5%(W/W) consumption evenly join in the soil of natural air drying, soil is with embodiment 1, soil is divided and installs in Plastic Drum, 3 kilograms, every barreled soil, after adding running water to make soil fully saturated, upper soll layer keeps the water layer of 10 centimetres, under room temperature, standing waterflooding is 14 days, contrasts the conventional waterflooding of soil for not adding wheat stalk.Outwell topsoil water after 14 days, the centrifugal 15min of soil 6000r/min by waterflooding, obtain supernatant, the aseptic filter membrane by supernatant by 0.22 μ m obtains aseptic decomposition liquid, adopts sessile drop method to measure the impact of decomposition liquid on the withered germ of water-melon conidia germination.
In accompanying drawing 7, withered germ of water-melon spore germination situation when A figure means not add wheat straw decomposition liquid (being the clear water contrast), B figure is withered germ of water-melon spore germination situation after interpolation wheat straw decomposition liquid.The microscopic examination result shows: after 8 hours, do not add the clear water contrast of decomposition liquid, the withered germ of water-melon germination rate is more than 95%, and the conidial germination rate of withered germ of water-melon of processing through wheat stalk decomposition liquid is only 15-20%.Be that A figure spore germination rate is high, B figure does not almost have spore germination, and visible, to withered germ of water-melon, spore germination has stronger inhibitory action to wheat stalk decomposition liquid.
Claims (2)
1. a method of utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable, it is characterized in that: after agricultural crop straw is applied to field, soil is carried out to submerging treatment, concrete steps are: soil temperature is more than 20 ℃, by the residual body of preceding crop in field, remove clean, the field surrounding is played to approximately 20 centimetres of high ribs with fortifield village, the agricultural crop straw that is crushed to the 10-15 centimetre length is evenly spread fertilizer over the fields to field, then 18-20 centimetre of soil ploughing is dark, with irrigation water, field is carried out to submerging treatment 5 days, during waterflooding, the field depth of water remains on the soil layer surface more than 5 centimetres, dark by soil rotary tillage 18-20 centimetre after 5 days, continue to keep waterflooding 15 days, when being reduced to 0-40mv, finishes soil redox potential waterflooding, after drying, soil natural applies chemical fertilizer, again by the crop of planting after soil rotary tillage, the amount of application of chemical fertilizer is: 30-40 kilogram urea and 20-30 kilogram potassium phosphate fertilizer.
2. the method for utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable according to claim 1, it is characterized in that: described agricultural crop straw refers to wheat, paddy rice or maize straw, consumption is every mu of 400-600 kilogram, stalk moisture 12-14%.
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| CN108059492A (en) * | 2017-12-06 | 2018-05-22 | 江苏省农业科学院 | A kind of method that vegetables residuum original position, quick Fertilizer Transformed utilize |
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| CN110583137A (en) * | 2019-09-24 | 2019-12-20 | 湖南省蔬菜研究所 | Method for preventing and treating pepper diseases and increasing yield and improving quality, organic preparation and preparation method thereof |
| CN113796270A (en) * | 2021-10-08 | 2021-12-17 | 湖南省农业科学院 | Method for preventing and controlling rice planthopper outbreak based on depth alternate flooding irrigation |
| CN113796270B (en) * | 2021-10-08 | 2022-09-13 | 湖南省农业科学院 | Method for preventing and controlling rice planthopper outbreak based on depth alternate flooding irrigation |
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