CN103468604A - Bacillus subtilis and application thereof - Google Patents
Bacillus subtilis and application thereof Download PDFInfo
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- CN103468604A CN103468604A CN201310327938XA CN201310327938A CN103468604A CN 103468604 A CN103468604 A CN 103468604A CN 201310327938X A CN201310327938X A CN 201310327938XA CN 201310327938 A CN201310327938 A CN 201310327938A CN 103468604 A CN103468604 A CN 103468604A
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- bacillus subtilis
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- bioactive peptide
- peptide protein
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Abstract
Description
Claims (6)
- Subtilis ( bacillus subtilis) bacterial strain KF-2, deposit number is CGMCCNo.7690.
- 2. bacillus subtilis strain KF-2 as claimed in claim 1 is rich in the application in the bioactive peptide protein fodder in fermentative production.
- 3. one kind is utilized bacillus subtilis strain KF-2 fermentative production as claimed in claim 1 to be rich in the method for bioactive peptide protein fodder, it is characterized in that comprising following processing step:1) described bacillus subtilis strain KF-2 is passed through to plate culture medium activation, seed shake-flask culture, one-level shake-flask culture or fermentor tank amplification culture step by step, obtain seed liquor;2) seed liquor obtained through step 1) is seeded in dregs of beans carries out solid state fermentation;3) by step 2) tunning that obtains dried, pulverized, and obtains being rich in the bioactive peptide protein fodder.
- 4. a kind of method of utilizing bacillus subtilis strain KF-2 fermentative production to be rich in the bioactive peptide protein fodder as claimed in claim 3, is characterized in that described step 2) in inoculum size be 8~12% of dregs of beans weight.
- 5. a kind of method of utilizing bacillus subtilis strain KF-2 fermentative production to be rich in the bioactive peptide protein fodder as claimed in claim 3, it is characterized in that described step 2) in solid state fermentation conditions be: during inoculation, fully stir, in fermenting process, monitor temperature changes, temperature is during higher than 50 ℃, carry out the stirring processing, fermentation time is 48 h.
- 6. a kind of bacillus subtilis strain KF-2 fermentative production as claimed in claim 3 is rich in the method for bioactive peptide protein fodder, it is characterized in that in described step 3), tunning is dried to moisture≤12%, smashing fineness 40~60 orders.
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CN201310327938.XA CN103468604B (en) | 2013-07-31 | 2013-07-31 | Bacillus subtilis and application thereof |
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CN201310327938.XA CN103468604B (en) | 2013-07-31 | 2013-07-31 | Bacillus subtilis and application thereof |
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CN103468604A true CN103468604A (en) | 2013-12-25 |
CN103468604B CN103468604B (en) | 2015-07-15 |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103766586A (en) * | 2014-02-07 | 2014-05-07 | 范红玉 | Method for promoting formation of bacillus subtilis spores by using metal ions |
CN105603031A (en) * | 2016-03-09 | 2016-05-25 | 广东海洋大学 | Aquatic protein active peptide preparing method without adopting high-temperature treatment |
CN107535671A (en) * | 2017-09-27 | 2018-01-05 | 浙江大学 | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate |
CN107821844A (en) * | 2017-11-30 | 2018-03-23 | 山东和美集团有限公司 | Improve feed of duck's egg quality and preparation method thereof |
CN108497174A (en) * | 2017-02-23 | 2018-09-07 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus subtilis and produces fermented feed |
CN109321619A (en) * | 2018-09-17 | 2019-02-12 | 江苏大学 | The method that pulsed magnetic field sterilization combines high temperature bacterium solid state fermentation grouts to prepare oligopeptide |
CN109965082A (en) * | 2019-04-15 | 2019-07-05 | 广东大广生物科技有限公司 | A kind of child care pig fermented feed and preparation method thereof |
CN113801825A (en) * | 2021-10-21 | 2021-12-17 | 厦门海嘉成生物科技有限公司 | Bacillus subtilis for producing active peptide and application thereof |
CN114231445A (en) * | 2021-11-30 | 2022-03-25 | 唐山仟客莱生物科技有限公司 | Mixed fermentation medium of composite probiotics and application thereof |
-
2013
- 2013-07-31 CN CN201310327938.XA patent/CN103468604B/en active Active
Non-Patent Citations (3)
Title |
---|
何勇锦 等: "枯草芽孢杆菌KJ发酵豆粕制备新型蛋白肽饲料的研究", 《云南民族大学学报(自然科学版)》 * |
刘晓艳 等: "固态发酵高温豆粕制备多肽饲料的最优发酵工艺条件研究", 《大豆科学》 * |
李舒宇 等: "枯草芽孢杆菌固态发酵高温豆粕的工艺优化", 《食品与机械》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103766586A (en) * | 2014-02-07 | 2014-05-07 | 范红玉 | Method for promoting formation of bacillus subtilis spores by using metal ions |
CN105603031A (en) * | 2016-03-09 | 2016-05-25 | 广东海洋大学 | Aquatic protein active peptide preparing method without adopting high-temperature treatment |
CN108497174A (en) * | 2017-02-23 | 2018-09-07 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus subtilis and produces fermented feed |
CN107535671A (en) * | 2017-09-27 | 2018-01-05 | 浙江大学 | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate |
CN107535671B (en) * | 2017-09-27 | 2020-09-11 | 浙江大学 | Microbial fermentation yellow wine lees feed for improving rumen protein utilization rate and preparation method thereof |
CN107821844A (en) * | 2017-11-30 | 2018-03-23 | 山东和美集团有限公司 | Improve feed of duck's egg quality and preparation method thereof |
CN109321619A (en) * | 2018-09-17 | 2019-02-12 | 江苏大学 | The method that pulsed magnetic field sterilization combines high temperature bacterium solid state fermentation grouts to prepare oligopeptide |
CN109965082A (en) * | 2019-04-15 | 2019-07-05 | 广东大广生物科技有限公司 | A kind of child care pig fermented feed and preparation method thereof |
CN113801825A (en) * | 2021-10-21 | 2021-12-17 | 厦门海嘉成生物科技有限公司 | Bacillus subtilis for producing active peptide and application thereof |
CN114231445A (en) * | 2021-11-30 | 2022-03-25 | 唐山仟客莱生物科技有限公司 | Mixed fermentation medium of composite probiotics and application thereof |
CN114231445B (en) * | 2021-11-30 | 2024-03-26 | 唐山仟客莱生物科技有限公司 | Mixed fermentation medium of composite probiotics and application thereof |
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CN103468604B (en) | 2015-07-15 |
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GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
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Denomination of invention: A Bacillus subtilis and its application Effective date of registration: 20221114 Granted publication date: 20150715 Pledgee: Hangzhou United Rural Commercial Bank Co.,Ltd. Haining sub branch Pledgor: ZHEJIANG COFINE BIOTECH. Inc.,Ltd. Registration number: Y2022980021846 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230823 Granted publication date: 20150715 Pledgee: Hangzhou United Rural Commercial Bank Co.,Ltd. Haining sub branch Pledgor: ZHEJIANG COFINE BIOTECH. Inc.,Ltd. Registration number: Y2022980021846 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Bacillus subtilis and its application Effective date of registration: 20230901 Granted publication date: 20150715 Pledgee: Hangzhou United Rural Commercial Bank Co.,Ltd. Haining sub branch Pledgor: ZHEJIANG COFINE BIOTECH. Inc.,Ltd. Registration number: Y2023980054897 |