CN103463220A - Standardized processing technology of Chinese herbal piece stewed aconite - Google Patents

Abstract

The invention discloses a standardized processing technology of a Chinese herbal piece stewed aconite. The standardized processing technology is characterized by comprising the following steps of: soaking salted aconite, taking out and draining water when soaking water is 25 DEG C and electrical conductivity is lowered to below 6000mu s/cm; uniformly and flatly spreading salted aconite with expanded part up in clean and burnt fine bran ash with thickness of 10cm-40cm, spreading a plurality of layers of clean ginger slices over the bran ash, and flatly covering 1-5 pieces of coarse paper on the ginger slices, spreading a layer of fine clean bran ash with thickness of 3cm-10cm on the coarse paper, and flatly spreading dry bran hulls with thickness of 5cm-30cm on the ash; igniting the dry bran hulls at four corners, controlling the upper-end temperature of the aconite to 110-180 DEG C, controlling the lower-end temperature of the aconite to 70-130 DEG C, continuously baking for 36-72 hours, taking out the aconite when the bran ash is cooled, placing in a heating container, continuously steaming for 6-12 hours, pouring out, spreading and airing to the room temperature, slicing, and drying at 60 DEG C-80 DEG C until the moisture content is 5%-13%, thus obtaining the Chinese herbal piece stewed aconite. The standardized processing technology has obvious advantages of reducing toxicity, enhancing efficacy and reducing active ingredient loss in comparison with a normal steaming method and a cooking method, so that the stewed aconite pieces are high-quality.

Description

A kind of prepared slices of Chinese crude drugs are simmered the standardization processing procedure of Radix Aconiti Lateralis Preparata

Technical field

The invention belongs to the field of Chinese medicines, relate to the standardization processing procedure that a kind of prepared slices of Chinese crude drugs are simmered Radix Aconiti Lateralis Preparata.

Background technology

Radix Aconiti Lateralis Preparata is the ranunculaceae plant Aconitum carmichjaelii Debx. acormitum Carmichaelidebx. the processed goods of daughter root.Acrid in the mouth, sweet, large heat, poisonous.There is recuperating depleted YANG and rescuing the patient from collapse, mend fire supporing yang, the effect of dispersing cold for relieving pain.Its property of medicine is just dry, walks and does not keep, and is widely used in treating the YANG-depletion collapse, cold extremities faint pulse, sexual impotence, cold womb, the diseases such as trusted subordinate's cold type of pain.Modern study proves that it has the effects such as heart tonifying, antiinflammatory, analgesia, clinical heart failure, shock and the bradyarrhythmia etc. of being used for the treatment of.Radix Aconiti Lateralis Preparata is key medicine simply and the high medicine that tcm clinical practice is commonly used, praised highly by numerous well-known doctors at all times.As Ming Dynasty well-known doctor Zhang Jingyue is classified Radix Aconiti Lateralis Preparata as one of " four-dimensional in medicine ".Modern well-known doctor Wu Peiheng classifies Radix Aconiti Lateralis Preparata one of as Chinese medicine ten large chief commanders.According to statistics, in 82 bright clear cases, Radix Aconiti Lateralis Preparata compound recipe 2108 head occur altogether, cure mainly disease and reach 138.

Radix Aconiti Lateralis Preparata is used as medicine after mostly concocting.From Han dynasty, the Radix Aconiti Lateralis Preparata concocting method approximately has more than 70 to plant so far, and application accessory reaches tens kinds, and the decoction pieces kind has tens kinds of Radix Aconiti Lateralis Preparata, Radix Aconiti Lateralis Preparata, RADIX ACONITI LATERALIS PREPARATA, gun tab, stewing Radix Aconiti Lateralis Preparata, yellow Radix Aconiti Lateralis Preparata, RADIX ACONITILATERALIS PREPARATA, hexagram Radix Aconiti Lateralis Preparatas etc.Chinese Pharmacopoeia 2010 editions only records first four kinds.Major part method, because coming from place, rarely has Revision, causes these to have local characteristic and traditional processing technique that can produce specially good effect passes into silence just gradually, in the Critical Condition of atrophy.The concocting method the earliest as Radix Aconiti Lateralis Preparata, simmer method (the ancient big gun method that claims) faces lost condition equally.In fact, the Radix Aconiti Lateralis Preparata simmer method is compared other concocted methods and is had clear superiority:

(1) simmering method characteristics and the Radix Aconiti Lateralis Preparata property of medicine matches.Belonging to the stewing method of fire preparing category and the impatient and hot effect characteristics perfection of Radix Aconiti Lateralis Preparata agrees with.The Jing-Yue Complete Works cloud: the property of Radix Aconiti Lateralis Preparata, just anxious and heat, system is with inappropriate, and difficult cloud is nontoxic, therefore the doing in the proper way of wish system.Husband sky purgation system poison person, without wonderful, in fire, why fire can make malicious person, can remove from office the property of thing.Therefore meet fire with gas, lose its gas, flavor and meet fire, lose its flavor, and just the person removes from office that it is firm, and gentle person loses that it is gentle.Therefore make attached method, but with plain boiled water, boil extremely ripe, also entirely lose pungent, and its hot all mistake, is shape as edible as Radix Raphani, still the foot worry of what poison? Japanese philosopher Miscanthus sacchariflorus (Maxim) Benth et Hook f gives birth to and once said to come: though Radix Aconiti Lateralis Preparata is simmered the property that does not subtract its large heat.Hong Kong Du is thick firmly to be thought, ancients adopt dirt to wrap up in the stewing method, close the artistic conception of symbol " in fire volt soil " most, bias toward the property of reinforcements " volt fire " or " treating YANG within YIN ", and enhancing lies prostrate the effect that fire consolidates.Now proved that violent in toxicity in Radix Aconiti Lateralis Preparata-di esters aconitine is met Gao Reyi destroyed, be decomposed into the alkaloid that toxicity is less, visible ancient medicine is processed Radix Aconiti Lateralis Preparata with fire, truly has certain science reason.

(2) be conducive to preserve drug effect.The fire-making methods such as big gun the earliest, stewing not only can reduce the toxicity of Radix Aconiti Lateralis Preparata, than the Radix Aconiti Lateralis Preparata boiling, steam, avoiding the loss of effective ingredient, preserve the aspect such as drug effect very large benefit is arranged.

(3) stewing Radix Aconiti Lateralis Preparata clinical practice basis is deep, acts on conspicuous characteristics.Radix Aconiti Lateralis Preparata concocting method the earliest is big gun method (belonging to fire-making method, close with the stewing method), and clinical practice is with a long history.Through successive dynasties doctor clinical practice, show, the ripe attached property of boiling is slow is advisable with tonify deficiency, and after the system of simmering, though just dry property has slowed down, YANG invigorating and antalgic effect are strengthened, and impel it to walk table to strengthen the table sun and to draw all medicines and pass through." medicine is refined " said: " its Z-TEK of survivor is violent, and the treating the disorder in the interior sun is when critically ill; Big gun its property is slightly slow, walks table and divides with the capable water of warming the meridian "." careful vegetarian posthumous papers " meaning: " face is wrapped up in stewing person, walks and does not keep, and its gesture is up, can tonifying YANG in table "." the doctor ancestor says approximately " meaning: " draw all medicines and pass through, face is wrapped up in fire and simmered ".Simmering this effect characteristics of Radix Aconiti Lateralis Preparata is that other Radix Aconiti Lateralis Preparata processed products can't replace.

To make a general survey of ancient times Radix Aconiti Lateralis Preparata big guns and to process purpose be not exclusively in order detoxifying, have plenty of the treatment of following.At present research emphasis is mainly Radix Aconiti Lateralis Preparata boiling method and Powder Made by Steamed Method, and more can embody the simmer method research that the Radix Aconiti Lateralis Preparata invigorating YANG helps fiery characteristic and ancients to concoct intention, lacks.Therefore, by modern analysis means and method, to simmering the Radix Aconiti Lateralis Preparata processing procedure, furtherd investigate, thereby the industrialization and the large-scale production that realize simmering Radix Aconiti Lateralis Preparata promote local Chinese Medicine Industry fast development.This is an urgent demand of inheriting with development Chinese medicine tradition processing procedure, is also to promote the march toward committed step of standardization, standardization and process of industrialization of Chinese medicine processing.

Summary of the invention

The object of the present invention is to provide a kind of prepared slices of Chinese crude drugs to simmer the standardization processing procedure of Radix Aconiti Lateralis Preparata.By the preferred processing procedure parameter of Scientific experimental design method, and realize in the steps below purpose of the present invention.

Concocting method of the present invention is as follows:

1, a kind of prepared slices of Chinese crude drugs are simmered the standardization processing procedure of Radix Aconiti Lateralis Preparata, and concocting method is as follows:

(1), get Radix Aconiti Lateralis Preparata, select stepping, in placing container, add water to and flood Radix Aconiti Lateralis Preparata fully, between soak period, change water 2-4 every day, when soaking water at 25 ℃, electrical conductivity is down to 6000 μ s/cm when following, takes out, drain the water step (1) make thing;

(2), by step (1) make thing expand the part upward, evenly be laid in the clean thin bran ash burnt that 10-40cm is thick, cover in the above the clean ginger slice of some layers, the thing that makes by every 100kg step (1) adds 10-16kg Rhizoma Zingiberis Recens, on ginger slice, the flat 1-5 that covers opens toilet paper, repave the clean thin bran ash that one deck 3-10cm is thick on paper, the thick dry bran shell of tiling 5-30cm on ash;

(3), ignite at the dry bran shell four angle point fire of tiling, control the upper end temperature of Radix Aconiti Lateralis Preparata at 110-180 ℃, the lower end temperature is at 70-130 ℃, uninterruptedly simmers 36-72 hour processed, treats that the bran ash is cooling, and the taking-up Radix Aconiti Lateralis Preparata obtains the thing that makes of step (3);

(4), the thing that makes of step (3) is placed in heating container, uninterruptedly steam 6～12 hours, incline and, airing to room temperature obtains the thing that makes of step (4);

(5), get the thing that makes of step (4), cut the vertical thin slice that 1-4mm is thick, be dried to water content 5%～13% at 60～80 ℃, obtain.

Preferably be that concocting method is as follows:

(1), get Radix Aconiti Lateralis Preparata, select stepping, in placing container, add water to and flood Radix Aconiti Lateralis Preparata fully, between soak period, change water 2-4 every day, when soaking water at 25 ℃, electrical conductivity is down to 4000 μ s/cm when following, takes out, drain the water step (1) make thing;

(2), by step (1) make thing expand the part upward, evenly be laid in the clean thin bran ash burnt that 20-30cm is thick, cover in the above the clean ginger slice of some layers, the thing that makes by every 100kg step (1) adds the 12kg Rhizoma Zingiberis Recens, on ginger slice, the flat 2-4 that covers opens toilet paper, repave the clean thin bran ash that one deck 5-8cm is thick on paper, the thick dry bran shell of tiling 10-15cm on ash;

(3), ignite at the dry bran shell four angle point fire of tiling, control the upper end temperature of Radix Aconiti Lateralis Preparata at 140-170 ℃, the lower end temperature is at 80-110 ℃, uninterruptedly simmers 36-48 hour processed, treats that the bran ash is cooling, and the taking-up Radix Aconiti Lateralis Preparata obtains the thing that makes of step (3);

(4), the thing that makes of step (3) is placed in heating container, uninterruptedly steam 8～10 hours, incline and, airing to room temperature obtains the thing that makes of step (4);

(5), get the thing that makes of step (4), cut the vertical thin slice that 2-3mm is thick, be dried to water content 6%～12% at 60～80 ℃, obtain.

Advantage of the present invention:

(1) effective ingredient and pharmacodynamics index aspect.In the Radix Aconiti Lateralis Preparata that adopts simmer method to prepare, diester-type and monoester alkaloid content are all higher than steamed method and boiling method; Wherein Mn/Fe content is apparently higher than steamed method, close with the boiling method, and the low ferrum of high manganese is the elemental basis of the warm property of medicine; In the antiinflammatory experiment of the analgesic experiment of hot plate method, writhing method and Mus ear swelling method, simmer the Radix Aconiti Lateralis Preparata anti-inflammatory and analgesic effect rapid-action, action intensity is greater than all the other two kinds of Radix Aconiti Lateralis Preparata processed products; By acute toxicity testing, shown, the toxicity of three kinds of Radix Aconiti Lateralis Preparata processed products is more approaching.Therefore, contained chemical composition and pharmacodynamic index angle from the prepared slices of Chinese crude drugs, known the inventive method has clear superiority in steamed method and boiling method commonly used aspect attenuation synergistic, minimizing loss of effective components, simmers Radix Aconiti Lateralis Preparata best in quality, with the process of preparing Chinese medicine intention of traditional Radix Aconiti Lateralis Preparata, matches.

(2) selling point.Simmer Radix Aconiti Lateralis Preparata after floating, add Rhizoma Zingiberis Recens stewing system, though its firm dry property slows down, YANG invigorating and antalgic effect are strengthened, and impel it to walk table and show sun and draw all medicines and pass through to strengthen, and toxicity is little, few side effects, safe and effective through the successive dynasties clinical practice.

The accompanying drawing explanation

Fig. 1 is mesaconitine of the present invention, aconitine, hypaconitine reference substance HPLC figure.

Fig. 2 is diester-type alkaloids HPLC figure in Radix Aconiti Lateralis of the present invention.

Fig. 3 is diester-type alkaloids HPLC figure in Radix Aconiti Lateralis Preparata of the present invention.

Fig. 4 is that the present invention simmers diester-type alkaloids HPLC figure in Radix Aconiti Lateralis Preparata.

Fig. 5 is diester-type alkaloids HPLC figure in the steamed Radix Aconiti Lateralis Preparata of the present invention.

Fig. 6 is diester-type alkaloids HPLC figure in boiling Radix Aconiti Lateralis Preparata of the present invention.

Fig. 7 benzoyl aconine of the present invention, benzoyl hypo-aconine reference substance HPLC figure.

Monoester alkaloid HPLC figure in Fig. 8 Radix Aconiti Lateralis of the present invention.

Monoester alkaloid HPLC figure in Fig. 9 Radix Aconiti Lateralis decoction liquor of the present invention.

Monoester alkaloid HPLC figure in Figure 10 Radix Aconiti Lateralis Preparata of the present invention.

Figure 11 the present invention simmers monoester alkaloid HPLC figure in Radix Aconiti Lateralis Preparata.

Monoester alkaloid HPLC figure in Figure 12 boiling Radix Aconiti Lateralis Preparata of the present invention.

Monoester alkaloid HPLC figure in the steamed Radix Aconiti Lateralis Preparata of Figure 13 the present invention.

In figure, 1-is mesaconitine, and 2-is aconitine, and 3-is hypaconitine, and 4-is benzoyl aconine, and 5-is benzoyl hypo-aconine.

The specific embodiment

The present invention is by the following examples can the invention will be further described, yet scope of the present invention is not limited to following embodiment.

Embodiment 1 prepared slices of Chinese crude drugs are simmered the standardization processing procedure of Radix Aconiti Lateralis Preparata:

The first step: get Radix Aconiti Lateralis Preparata, select stepping, in placing container, add water to and flood Radix Aconiti Lateralis Preparata fully, soak 48～94 hours, change water every day 2 times, wait soaking electrical conductivity of water, be down to 4000 μ s/cm (25 ℃) when following, take out, drain the water, standby.

Second step: get the clean Radix Aconiti Lateralis Preparata drained, it is expanded to part upward, evenly be laid in the clean thin bran ash burnt that 20cm is thick, cover in the above the clean ginger slice of some layers, the thing that makes by every 100kg step 1 adds the 12kg Rhizoma Zingiberis Recens, 2 toilet paper of flat covering on ginger slice, repave the clean thin bran ash that one deck 5cm is thick on paper, a small amount of dry bran shell of tiling on ash; Dry bran shell four angle point fire in tiling ignite, and control the upper end temperature of Radix Aconiti Lateralis Preparata at 140-160 ℃, and the lower end temperature is at 90-100 ℃, and uninterrupted the stewing made 48 hours, treats that the bran ash is cooling, takes out Radix Aconiti Lateralis Preparata.

The 3rd step: get the Radix Aconiti Lateralis Preparata of simmering after making, be placed in heating container, uninterruptedly steam 8 hours, incline and, airing, to room temperature, is cut the vertical thin slice that 2-3mm is thick, at 70 ℃, is dried to water content 6%～9%, quality examination, packing.

Research: different Radix Aconiti Lateralis Preparata processed product qualities relatively

1. instrument and material

1.1 instrument Shimadzu LC-10ATvp high performance liquid chromatograph (Shimadzu company); N2000 Data Processing in Chromatography Workstation (Zhejiang University's intelligent information Graduate School of Engineering); The automatic aqua bi-distilling apparatus of BSZ-2 type (Shanghai Botong); ST-400 column oven (Tianjin first Deco instrument company); Ultrasonic cleaner (Kunshan ultrasonic instrument company limited); METTLER-TOLEDD ABS265-S type electronic balance (Switzerland); The full-automatic thin layer making sheet of TD-II device (wise man of Shanghai section); IXUS750 type Canon digital camera (CANON); DHG101-7A electric heating blowing-type drying baker (Ying Yu of Gongyi City gives magnificent instrument plant); Tu-1901 type ultraviolet-uisible spectrophotometer (Beijing is general analyses); SX2 type muffle furnace (Shanghai Si Erda scientific instrument company limited); DZF-2001 type vacuum drying oven (the holy glad tech in Shanghai); WFH-201B ultraviolet transmission reflectometer (above Nereid section); The accurate AC cleaning regulated device (regulator factory, Shanghai City) of JJW-5KVA; K, Zn, Cu, Fe, Mn, Ni element hollow cathode lamp (Beijing Non-Ferrous Metal Research General Academy's production); TAS-990 atomic absorption spectrophotometer (Beijing is general analyses); ZH-YLS-6B intelligence hot-plate instrument (Anhui is China just).

1.2 material Radix Aconiti Lateralis Preparata processed product sample: simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata (Lignum cinnamomi camphorae side method), boiling Radix Aconiti Lateralis Preparata (pharmacopeia method), self-control; White mice, provided by the Jiangxi College of Traditional Chinese Medicine Experimental Animal Center; Reference substance: mesaconitine (110799-200404), aconitine (110720-200410), hypaconitine (110798-200404), benzoyl aconine (111794-200901), benzoyl hypo-aconine (111796-200901), purchased from Nat'l Pharmaceutical & Biological Products Control Institute; K, Zn, Cu, Fe, Mn, Ni standard reserving solution lmg/mL (State Standard Matter Research Centre); Silica gel G (Chinese Qingdao Haiyang Chemical Industry Group Corp.); Reagent is that chromatographically pure, other are analytical pure except methanol, acetonitrile for HPLC.

2. method and result

2.1 different Radix Aconiti Lateralis Preparata processed product mesaconitine, mesaconitine, hypaconitine assay

Method: adopt HPLC to measure Radix Aconiti Lateralis Preparata mesaconitine, mesaconitine, hypaconitine content.

2.1.2 the preparation of solution

2.1.2.1 mix the preparation precision of reference substance solution, to take mesaconitine, aconitine, hypaconitine reference substance appropriate, adds methanol and make every 1 mL respectively containing the mixing reference substance solution of 16 μ g, 25.6 μ g, 32.1 μ g, standby.

2.1.2.2 the preparation of need testing solution takes approximately about about 3 g of 2 g and Radix Aconiti Lateralis Preparata processed product coarse powder of 0.1 g, Radix Aconiti Lateralis Preparata coarse powder of Radix Aconiti Lateralis coarse powder, precision weighing, put respectively in 150 mL tool plug conical flasks, add strong aqua ammonia 3 mL moistening 0.5 h, add the close plug of ether 100 mL, placement is spent the night, next day, ultrasonic 15 min, filtered, then filter with 30 mL ether gradation washing filtering residues, merging filtrate and washing liquid, 40 ℃ of water-baths volatilize, and the dissolve with methanol residue also is settled to 2 mL, shakes up, with 0.45 μ m microporous filter membrane, filter before use, standby.

2.1.3 chromatographic condition chromatographic column: Venusil XBP-C ₁₈post (5 μ m, 4.6 * 250mm); Mobile phase: acetonitrile-20 mmolL ^-1naH ₂pO ₄(phosphoric acid is adjusted pH4.0) (50:50) (includes 10 mmolL ^-1sodium lauryl sulphate); Flow velocity: 1.0mL/min; Detect wavelength: 235 nm; Column temperature: 35 ℃.

Precision is mixed reference substance solution 10 μ L under drawing 2.2, each 20 μ L of need testing solution carry out chromatography, records chromatogram.The results are shown in Figure 1-6.

Mix reference substance solution 1 mL, 2 mL, 4 mL, 6 mL, 8 mL, 10 mL and put in 10 mL measuring bottles 2.1.4 linear relationship is investigated accurate absorption the respectively, add methanol and be diluted to scale, shake up, accurate 20 μ L that draw respectively, the injection liquid chromatography, measure successively.With sample size X(μ g) peak area A is carried out to linear regression, regression equation is respectively: mesaconitine: a=683 834 x-216.28, r=0.9 998; Aconitine: a=816 632 x– 520, r=0.9 998; Hypaconitine: a=651 136 x-811.65, r=0.9 999.Known mesaconitine is the good linear relation at 0.032～0.32 μ g, aconitine at 0.0 512～0.512 μ g, hypaconitine peak area and sample size in 0.0642～0.642 μ g scope.

2.1.5 precision test is drawn the mixing reference substance solution 20 μ L of same concentration, repeats sample introduction and measures 5 times, records chromatogram, the RSD of mesaconitine, aconitine, hypaconitine peak area is respectively 0.89%, 0.10%, 0.85% as a result.

2.1.6 the stability test precision takes Radix Aconiti Lateralis sample 0.1 g, press 2.2.2 item below legal system available test sample solution, difference sampling and measuring after room temperature is placed 0 h, 2 h, 4 h, 8 h and 24 h, the RSD of mesaconitine, aconitine, hypaconitine peak area is respectively 1.38%, 1.10%, 1.97% as a result, shows that need testing solution is stable in 24 h.

2.1.7 the replica test precision takes 5 parts of Radix Aconiti Lateralis sample 0.1 g, presses 2.2.2 item below legal system available test sample solution, measures the mass fraction (ω of mesaconitine, aconitine, hypaconitine in results sample in accordance with the law _b) be respectively 597.904 μ gg ^-1, 100.876 μ gg ^-1, 585.072 μ gg ^-1, RSD is respectively 1.87%, 1.47%, 1.51%.

2.1.8 accurate Radix Aconiti Lateralis sample 0.05 g that takes 6 parts of known content of average recovery test, every part accurate respectively adds mixing reference substance solution 1 mL that every 1 mL is 30.4 μ g, 6.2 μ g, 29.8 μ g containing mesaconitine, aconitine, hypaconitine, press 2.2.2 item below legal system available test sample solution, measure in accordance with the law.Mesaconitine, aconitine, hypaconitine average recovery rate are respectively as a result: 97.95%, RSD1.97%; 97.41%, RSD1.08%; 97.72%, RSD1.04%.

2.1.9 sample size is measured accurate Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata and the 3 kinds of Radix Aconiti Lateralis Preparata race goods prepared by different concocting methods of taking respectively, presses 2.2.2 item below legal system available test sample solution.The accurate absorption mixed reference substance solution 10 μ L, each need testing solution 20 μ L, and the injection liquid chromatography, record chromatogram.Use external standard method, with the mass fraction (ω of mesaconitine (mesaaconitine), aconitine (aconitine), hypaconitine (hypaaconitine) in the calculated by peak area sample _b).The results are shown in Table 1.3 kinds of diester-type alkaloids assay results in table 1 sample (

)

Annotate: " 0 " is can't integration.

According to " stipulate under 2010 editions Radix Aconiti Lateralis Preparata items of Chinese pharmacopoeia, Radix Aconiti Lateralis Preparata contains diester-type alkaloids with mesaconitine (C ₃₃h ₄₅nO ₁₁), hypaconitine (C ₃₃h ₄₅nO ₁₀) and aconitine (C ₃₄h ₄₇nO ₁₁) the total amount meter, must not cross 0.020%.As shown in Table 1, in 3 kinds of Radix Aconiti Lateralis Preparata processed products, diester-type alkaloids content, all lower than statutory standards, but adopt to be simmered content total amount in Radix Aconiti Lateralis Preparata higher than other two kinds of methods, with steamed method relatively have utmost point significant difference ( p<0.05), with the boiling method relatively have utmost point significant difference ( p<0.01).Because diester-type alkaloids is toxic component, be also effective ingredient, simmer alkaloid difference in Radix Aconiti Lateralis Preparata and whether can cause the variation of drug effect to remain further to be furtherd investigate.

2.2 in different Radix Aconiti Lateralis Preparata processed products, monoester alkaloid content is measured

Method: adopt the HPLC method to measure monoester alkaloid content.

2.2.1 chromatographic condition chromatographic column: Venusil XBP-C18 post (5 μ m, 4.6 * 250mm); Mobile phase: acetonitrile-20 mmolL ^-1naH ₂pO ₄(triethylamine is adjusted pH6.5) (47:53) (includes 10 mmolL ^-1sodium lauryl sulphate); Flow velocity: 1.0ml/min; Detect wavelength: 235nm; Column temperature: 35 ℃.

2.2.2 the preparation of solution

2.2.2.1 the preparation of reference substance solution

Precision takes benzoyl aconine, the benzoyl hypo-aconine reference substance is appropriate, adds mobile phase and makes every 1ml respectively containing the mixed solution of 50 μ g, obtains.

2.2.2.2 the preparation of need testing solution

Get respectively Radix Aconiti Lateralis, the about 2g of Radix Aconiti Lateralis Preparata and each Radix Aconiti Lateralis Preparata processed product powder (crossing sieve No. three), accurately weighed, put in tool plug conical flask, add ammonia solution 3ml, precision adds the weighed weight supersound process of isopropyl alcohol-ethyl acetate (1:1) mixed solution 50ml(), at water temperature ultrasonic 30min below 25 ℃, let cool, weighed weight again, supply the weight of less loss with isopropyl alcohol-ethyl acetate (1:1) mixed solution, shake up, filter, precision measures subsequent filtrate 25ml, decompression and solvent recovery below 40 ℃ is to dry, the residue precision adds isopropyl alcohol-dichloromethane (1:1) mixed solution 3ml to dissolve, filter, get subsequent filtrate, obtain.

Get Radix Aconiti Lateralis 10g, add in flask, add 8 times of water gagings, weigh, decoct 2h, let cool, weigh, add the weight that water is supplied minimizing, centrifugal, get supernatant, obtain Radix Aconiti Lateralis decocting liquid.

2.2.3 measure

According to " appendix high performance liquid chromatography of Chinese pharmacopoeia version in 2010 (appendix VI D) is measured.Precision is drawn reference substance solution 10 μ l, each need testing solution 20 μ l respectively, and the injection liquid chromatography is measured, and obtains.The results are shown in Figure 7～Figure 13.

2.2.4 the assay of sample the results are shown in Table 2.

Monoester alkaloid content measurement result in table 2 sample

Annotate: " 0 " can't integration.

According to " stipulate under 2010 editions Radix Aconiti Lateralis Preparata items of Chinese pharmacopoeia, press dry product and calculate, this product is containing Benzoylmesaconine (C ₃₁h ₄₃nO ₁₀), benzoyl aconine (C ₃₂h ₄₅nO ₁₀) and benzoyl hypo-aconine (C ₃₁h ₄₃nO ₉) total amount, must not be less than 0.010%.It is contrast that benzoyl aconine, benzoyl hypo-aconine are take in this experiment, in Radix Aconiti Lateralis, Radix Aconiti Lateralis Preparata, all do not detect, and in Radix Aconiti Lateralis decocting liquid, content is very high, show that diester-type alkaloids is hydrolyzed into monoester alkaloid substantially, again verified the reasonability that Radix Aconiti Lateralis Preparata is decocted first attenuation, and monoester alkaloid may be the main effective ingredient of Radix Aconiti Lateralis Preparata in decoction; In 3 kinds of Radix Aconiti Lateralis Preparata processed products, the content total amount to be to simmer Radix Aconiti Lateralis Preparata for the highest, with steamed Radix Aconiti Lateralis Preparata have utmost point significant difference ( p<0.01), with the boiling Radix Aconiti Lateralis Preparata have utmost point significant difference ( p<0.001), but total amount is all on the low side.Reason may be relevant with the Radix Aconiti Lateralis Preparata raw materials quality.

2.3 Determination of trace elements in different Radix Aconiti Lateralis Preparata processed products

Method: adopt atomic absorption spectroscopy determination Radix Aconiti Lateralis Preparata K, Zn, Cu, Fe, Mn, Ni content.

2.3.1 the preparation of solution

2.3.1.1 the preparation of standard solution

Prepare respectively the series standard working solution of each element with K, Zn, Cu, Fe, Mn, Ni standard reserving solution.

1. K concentration of standard solution gradient (μ g/ml): 0.250,0.500,1.500,2.500,5.000;

2. Zn concentration of standard solution gradient (μ g/ml): 0.050,0.100,0.200,0.500,1.000;

3. Cu concentration of standard solution gradient (μ g/ml): 0.050,0.100,0.200,0.500,1.000;

4. Fe concentration of standard solution gradient (μ g/ml): 0.250,0.500,1.500,2.500,5.000;

5. Mn concentration of standard solution gradient (μ g/ml): 0.050,0.100,0.200,0.500,1.000;

6. Ni concentration of standard solution gradient (μ g/ml): 0.050,0.100,0.300,0.500,1.000.

2.3.1.2 the preparation of sample solution

Take each 3 parts of each Radix Aconiti Lateralis Preparata sample powder 1g, accurately weighed, be placed in respectively clean 250ml conical flask, add 20～30mL 3:1 (v/v) HNO3, mono-HClO4 nitration mixture, upper cover table ware.Be placed in hot digestion on electric hot plate or electric sand-bath.When acid solution is very few as do not digested well, then add several milliliters of mixed acid digestion liquid, continue hot digestion, until water white transparency.Add several ml deionized water, heating is to remove unnecessary nitric acid.When the liquid in beaker approaches 2～3mL, take off cooling.Wash and shift in the 100mL measuring bottle with deionized water, add deionized water and be settled to scale, obtain.

2.3.2 instrument working condition

The Flame Atomic Absorption Spectrometry instrument working condition table 3 that vides infra.

2.3.3 the drafting of standard curve

Under above-mentioned working condition, carry out the absorbance measuring of blank and K, Zn, Cu, Fe, Mn, the various concentration of Ni, system is drawn with absorbance the standard working curve to concentration automatically.The linear equation of each element, the standard working curve table 4 that vides infra.

Table 3 Flame Atomic Absorption Spectrometry instrument working condition

Table 4 linear equation and correlation coefficient charts

2.3.4 precision test

Get the Radix Aconiti Lateralis Preparata sample solution, under identical conditions, K, Zn, Cu, Fe, Mn, Ni6 kind element are respectively carried out to parallel assay, the precision of investigation method 11 times, the precision of each element is respectively 1.06%, 1.34% as a result, and 1.62%, 1.28%, 2.04%, 1.69%.

2.3.5 recovery test

Accuracy for verification sample processing and experimental technique, carry out recovery testu.Add the standard solution of a certain amount of this element in each sample, then measure its concentration.

The computing formula of the response rate:

P?%＝×100

Wherein: pfor the response rate; cfor measured value after mark-on; afor the raw sample measured value; bfor adding scale value.

The average recovery rate of K, Zn, Cu, Fe, Mn, Ni is respectively as a result: 102.7% (RSD=1.95%), 101.4% (RSD=2.38%), 95.7% (RSD=1.62%), 106.6% (RSD=1.57%), 94.7% (RSD=2.75%), 98.1% (RSD=2.01%).

2.3.6 sample determination interpretation of result

By method and condition under 2.3.1.2,2.3.2 item, sample is processed and the sample introduction analysis, 5 parts of parallel assays, average.According to the difference of constituent content and mensuration Element sensitivity, dilute respectively suitable multiple while measuring each element.According to 2.3.3 standard curve direct reading.The results are shown in Table 5.

Table 5 sample Determination of trace elements result (

)

Annotate: compare warp with Radix Aconiti Lateralis tcheck, * p<0.05.

Mn/Fe average ratio table in table 6 sample trace element

Grouping	Fe（mg/g）	Mn（mg/g）	Mn / Fe（%）
				Simmer the Radix Aconiti Lateralis Preparata group	0.176±0.067*	0.024±0.010	13.63*
Boiling Radix Aconiti Lateralis Preparata group	0.181±0.059*	0.015±0.006	8.28
				Steamed Radix Aconiti Lateralis Preparata group	0.142±0.091*	0.019±0.008	13.38*

Annotate: compare warp with boiling Radix Aconiti Lateralis Preparata group tcheck, * p<0.05.

As shown in Table 5, except the Fe element, micronutrient levels and Radix Aconiti Lateralis there was no significant difference in three kinds of Radix Aconiti Lateralis Preparata processed products, prompting is in concocting process, and Fe unit have a small amount of loss.In the Radix Aconiti Lateralis Preparata prepared three kinds of different concocting methods, the content of 6 kinds of trace element of stewing Radix Aconiti Lateralis Preparata is K>Fe>Zn>Mn>Cu>Ni successively, the content of 6 kinds of trace element of boiling Radix Aconiti Lateralis Preparata is K>Fe>Zn>Cu>Mn>Ni successively, and the content of steamed 6 kinds of trace element of Radix Aconiti Lateralis Preparata is K>Fe>Zn>Mn>Cu>Ni successively.K content is followed successively by boiling Radix Aconiti Lateralis Preparata group>stewing Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group; Fe content is followed successively by boiling Radix Aconiti Lateralis Preparata group>stewing Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group; Zn content is followed successively by steamed Radix Aconiti Lateralis Preparata group>stewing Radix Aconiti Lateralis Preparata group>boiling Radix Aconiti Lateralis Preparata group; Mn content is followed successively by simmers Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group>boiling Radix Aconiti Lateralis Preparata group; Cu content is followed successively by boiling Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group>stewing Radix Aconiti Lateralis Preparata group; Ni content is followed successively by boiling Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group>stewing Radix Aconiti Lateralis Preparata group.But content there was no significant difference between each relevant trace element in three kinds of Radix Aconiti Lateralis Preparata processed products, show that different concocting methods are close to identical on the impact of the contained trace element of Radix Aconiti Lateralis Preparata.

Li Xiaomin etc. [Li Xiaomin, Jia Renyong, Wang Jian, etc. the research of Chinese medicine different properties and inorganic elements relation. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1997,22 (8): 502～504] analyze and relatively think that high ferro, low manganese are the elemental basis of cold and cool class Chinese medicine; Gao Meng, low ferrum are the elemental basis that determines the Chinese medicine warm medicine of a warm nature most probably.In three kinds of Radix Aconiti Lateralis Preparata processed products, manganese/ferrum ratio is followed successively by simmers Radix Aconiti Lateralis Preparata group>boiling Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group, and whether this hot property power with Radix Aconiti Lateralis Preparata exists relation to remain further to be studied.

2.4 adopt relatively three kinds of analgesic activities of concocting Radix Aconiti Lateralis Preparata of hot plate method in mice

2.4.1 animal screening and grouping

Get 18～22 gram female mice numbers, each 1 to be placed on hot plate (55 ± 0.1 ℃) upper, mice on being placed on hot plate to the pain threshold of metapedes required time (second) as this Mus occurring licking.Allly lick the metapedes time and be less than 5 seconds or be greater than 30 seconds or the leaper gives it up.Qualified mice is divided into to 3 groups of administration groups and 1 group of normal saline blank group at random, 10 every group, repeat to survey its normality threshold, get two subnormal threshold of pain meansigma methodss, as pain threshold before this Mus administration.

2.4.2 the preparation of sample solution and administration

Take respectively that to simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata appropriate, add 12 times of amounts of water, heating extraction 1 hour, filter, and gets filtrate, is concentrated into every 1ml and is equivalent to the 1g crude drug, standby.

Adopt the gastric infusion mode, each organizes dosage is each 0.15 ml/lO g, and 1 times/day, for three days on end, the blank group gives isopyknic normal saline.

2.4.3 experimental result

Within 15,30,60 minutes after the last administration, survey respectively the mice pain threshold, for preventing scalding, setting deadline is 60 s.Calculate the threshold of pain and improve percentage rate.The results are shown in Table 7.

Threshold of pain raising percentage rate (%)=

* 100%

Three kinds of Radix Aconiti Lateralis Preparata processed product analgesic activities of table 7 hot plate method comparison (

, n=10)

Annotate: compare warp with the blank group tcheck, * p<0.05, * * p<0.01, * * * p<0.001.

As shown in Table 7,3 kinds of Radix Aconiti Lateralis Preparata processed product groups are after administration 30min, and pain threshold obviously improves, their threshold of pains improve percentage rate and blank group relatively have extremely significant difference ( p<0.001), show that the Radix Aconiti Lateralis Preparata processed product has certain inhibitory action to the thermostimulation induced pain.Wherein simmer the threshold of pain of Radix Aconiti Lateralis Preparata group 15min, 30min, 60min after administration and improve percentage rate and steamed Radix Aconiti Lateralis Preparata group, the comparison of boiling Radix Aconiti Lateralis Preparata group, all have utmost point significant difference ( p<0.01), and steamed Radix Aconiti Lateralis Preparata group, boiling Radix Aconiti Lateralis Preparata group each observing time point to improve percentage rate more approaching in the threshold of pain, there was no significant difference.

In 3 kinds of Radix Aconiti Lateralis Preparata processed products, simmer the pain threshold maximum of Radix Aconiti Lateralis Preparata group after administration 15min, and steamed Radix Aconiti Lateralis Preparata group, the pain threshold of boiling Radix Aconiti Lateralis Preparata group after administration 30min just increase obviously, show to simmer Radix Aconiti Lateralis Preparata rapid-action to the inhibitory action of thermostimulation induced pain, and effect is strong, and steamed Radix Aconiti Lateralis Preparata, the onset of boiling Radix Aconiti Lateralis Preparata are relatively slow, effect relaxes.

2.5 adopt relatively three kinds of analgesic activities of concocting Radix Aconiti Lateralis Preparata of mouse writhing method

2.5.1 the preparation of sample solution

Take respectively that to simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata appropriate, add 12 times of amounts of water, heating extraction 1 hour, filter, and gets filtrate, is concentrated into every 1ml and is equivalent to the 1g crude drug, standby.

2.5.2 experiment grouping and administration

Get 40 of male mices, be divided at random 3 groups of administration groups and 1 group of blank group.Adopt the gastric infusion mode, each administration group dosage is each 0.15 ml/lO g, and the blank group gives isopyknic normal saline.

2.5.2 experimental result

After administration 30 minutes, the equal lumbar injection 0.5% acetic acid 0.2ml/ of each Mus only.Observe each group in 15 minutes and writhing response (abdominal part indent, stretching, extension hind leg, buttocks are raised) number of times occurs, calculate and respectively organize suppression ratio.The results are shown in Table 8.

Suppression ratio (%)=

* 100%

Three kinds of Radix Aconiti Lateralis Preparata processed product analgesic activities of table 8 writhing method comparison ( , n=10)

Annotate: compare warp with the blank group tcheck, * p<0.05, * * p<0.01.

As shown in Table 8, simmer Radix Aconiti Lateralis Preparata group mouse writhing number of times and blank group relatively have significant difference ( p<0.05), all the other two groups of there was no significant differences.Three's Dichlorodiphenyl Acetate induced mice pain suppression ratio size is successively: simmer Radix Aconiti Lateralis Preparata>boiling Radix Aconiti Lateralis Preparata>steamed Radix Aconiti Lateralis Preparata, but there was no significant difference each other.Show that 3 kinds of Radix Aconiti Lateralis Preparata processed product Dichlorodiphenyl Acetate induced mice pain have certain inhibitory action, the inhibitory action of wherein simmering Radix Aconiti Lateralis Preparata is the strongest.

2.6 adopt relatively three kinds of antiinflammatory actions of concocting Radix Aconiti Lateralis Preparata of Mus ear swelling method

2.6.1 the preparation of sample solution

Take respectively that to simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata appropriate, add 12 times of amounts of water, heating extraction 1 hour, filter, and gets filtrate, concentrated, makes respectively the solution that every 1ml is equivalent to 1g crude drug and 2g crude drug, standby.

2.6.2 animal grouping and administration

Get 80 of the healthy mices of 2O ± 2g, be divided at random 8 groups: 1 group of aspirin positive controls, 1 group of blank group, simmer Radix Aconiti Lateralis Preparata high and low dose group, steamed Radix Aconiti Lateralis Preparata high and low dose group, boiling Radix Aconiti Lateralis Preparata high and low dose group.Wherein the positive controls dosage is 5 mg/kg, and the high dose group of each Radix Aconiti Lateralis Preparata processed product group is that 40g/kg, low dose group are 20g/kg.The blank group gives same volume normal saline.Successive administration 3 days.

2.6.3 experimental result

After the last administration, 30min only drips in the mouse right ear front-back with dimethylbenzene 0.05ml/.After lh, dislocation is put to death and to be cut ears, with the 8mm belt puncher, mouse ear is laid to round auricle with position, accurately weighs, and the difference of same mice left and right auricle of take is swelling.The results are shown in Table 9.

Three kinds of Radix Aconiti Lateralis Preparata processed product antiinflammatory actions of table 9 Mus ear swelling method comparison (

, n=10)

Annotate: compare warp with the blank group tcheck, * p<0.05, * * p<0.01.

As shown in Table 9, the swelling of positive controls and each administration group all is less than the blank group, wherein simmer Radix Aconiti Lateralis Preparata high dose group and blank group have significant difference ( p<0.05).Swelling there was no significant difference between positive controls and each administration group, but stewing Radix Aconiti Lateralis Preparata high and low dose group and steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata high dose group all are less than positive controls.In 3 kinds of Radix Aconiti Lateralis Preparata processed products, the swelling of simmering Radix Aconiti Lateralis Preparata high and low dose group all is less than steamed Radix Aconiti Lateralis Preparata group and boiling Radix Aconiti Lateralis Preparata group, simmer Radix Aconiti Lateralis Preparata group>boiling Radix Aconiti Lateralis Preparata group>steamed Radix Aconiti Lateralis Preparata group, wherein simmer Radix Aconiti Lateralis Preparata (high dose) group>stewing Radix Aconiti Lateralis Preparata (low dosage) group>boiling Radix Aconiti Lateralis Preparata (high dose) group>steamed Radix Aconiti Lateralis Preparata (high dose) group>boiling Radix Aconiti Lateralis Preparata (low dosage) group>steamed Radix Aconiti Lateralis Preparata (low dosage) group.

By above experimental result, show that 3 kinds of Radix Aconiti Lateralis Preparata processed products all can alleviate the mice caused by dimethylbenzene xylene ear thickness, there is certain antiinflammatory action, wherein respectively organize the high dose group effect and all be better than low dose group, act on the strongest for simmering the Radix Aconiti Lateralis Preparata group.

2.7 three kinds of acute toxicity tests of concocting the Radix Aconiti Lateralis Preparata product

2.7.1 the preparation of sample solution

Get Radix Aconiti Lateralis 3g, add 15 times of standing 15min of amount distilled water, more ultrasonic 30min, centrifugal, get supernatant, concentrated, make the solution that every 1ml is equivalent to the 0.075g crude drug; Take respectively that to simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata appropriate, add 5 times of amount distilled water, standing 15min, ultrasonic 30min, centrifugal, make respectively the solution that every 1ml is equivalent to the 2g crude drug, standby.

2.7.2 animal grouping and administration

Get 170 of the healthy mices of 2O ± 2g, weigh, be divided at random 17 groups.1 group of blank group, 4 dose levels groups of Radix Aconiti Lateralis and simmer Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, each 4 dose levels groups of boiling Radix Aconiti Lateralis Preparata.

1 group of Radix Aconiti Lateralis group with simmer the medicine groups such as Radix Aconiti Lateralis Preparata, steamed Radix Aconiti Lateralis Preparata, boiling Radix Aconiti Lateralis Preparata, before gavage, water 12 h are can't help in fasting, conventional 7 d that raise after administration, and dose levels is established 4 grades of 0.48 ml, 0.29 ml, 0.17 ml, 0.1 ml etc., within 1 day, successive administration is 5 times, every minor tick 3 hours.The blank group gives same volume normal saline.

Observe in time the situation of Mouse Weight, feed, water inlet, the activity of close observation mice, the mental status, diet and survival condition from administration for the first time.Record body weight, toxic reaction and death condition every day, dead mice is dissected and carries out perusal in time.

2.7.3 experimental result:

After gavage, tested mice is movable obviously to be reduced, and lazy moving, sleeping volt phenomenon occurs, and appetite descends obviously, the poisoning symptoms such as delay of response, and more early, symptom is more obvious for the higher appearance of dosage.Wherein Radix Aconiti Lateralis high dose group mice spits out white foams, and dead with tic.After stopping administration, 0.17 ml, 0.1 ml dosage group mice sign take a turn for the better gradually.Each organizes the main peak period of dead mouse during the first day administration, and next is mainly seen in administration 2 days, and after the 4th day, dead mouse obviously reduces, and the dead mice sign of small dose group recovers normal substantially.Mouse Weight variation and death condition are in Table 10.

Three kinds of Radix Aconiti Lateralis Preparata processed product the acute toxicity tests of table 10 ( )

By table 10, each administration group Mouse Weight weightening finish all is significantly less than the blank group, shows that Radix Aconiti Lateralis Preparata and processed product thereof all have certain toxicity.The mouse death rate of Radix Aconiti Lateralis high dose group and minimum dose group is respectively 100%, 0, and adopt its LD50 of BLISS method preresearch estimates to be: with log10 dose, the dead mouse probability is done to linear equation: y=1.4157x-0.2386, r=0.9914. is LD50=log ^-1[(0.5+0.2386)/1.4157]=4.14 g/kg. well below Radix Aconiti Lateralis Preparata process of preparing Chinese medicine group, shows that Radix Aconiti Lateralis toxicity is stronger due to its dosage.In 3 kinds of Radix Aconiti Lateralis Preparata processed product groups, the high dose group dosage has surpassed nearly 27 times of Radix Aconiti Lateralis high dose, shows that its toxicity is significantly smaller than Radix Aconiti Lateralis.And three groups of processed product group dead mouse percentage rate are all more approaching, weight gain rate there was no significant difference, show that three's acute toxicity is close.

Claims (2)

1. the prepared slices of Chinese crude drugs are simmered the standardization processing procedure of Radix Aconiti Lateralis Preparata, it is characterized in that concocting method is as follows:

(1), get Radix Aconiti Lateralis Preparata, select stepping, in placing container, add water to and flood Radix Aconiti Lateralis Preparata fully, between soak period, change water 2-4 every day, when soaking water at 25 ℃, electrical conductivity is down to 6000 μ s/cm when following, takes out, drain the water step (1) make thing;

(2), by step (1) make thing expand the part upward, evenly be laid in the clean thin bran ash burnt that 10-40cm is thick, cover in the above the clean ginger slice of some layers, the thing that makes by every 100kg step (1) adds 10-16kg Rhizoma Zingiberis Recens, on ginger slice, the flat 1-5 that covers opens toilet paper, repave the clean thin bran ash that one deck 3-10cm is thick on paper, the thick dry bran shell of tiling 5-30cm on ash;

(3), ignite at the dry bran shell four angle point fire of tiling, control the upper end temperature of Radix Aconiti Lateralis Preparata at 110-180 ℃, the lower end temperature is at 70-130 ℃, uninterruptedly simmers 36-72 hour processed, treats that the bran ash is cooling, and the taking-up Radix Aconiti Lateralis Preparata obtains the thing that makes of step (3);

(4), the thing that makes of step (3) is placed in heating container, uninterruptedly steam 6～12 hours, incline and, airing to room temperature obtains the thing that makes of step (4);

(5), get the thing that makes of step (4), cut the vertical thin slice that 1-4mm is thick, be dried to water content 5%～13% at 60～80 ℃, obtain.

2. a kind of prepared slices of Chinese crude drugs according to claim 1 are simmered the standardization processing procedure of Radix Aconiti Lateralis Preparata, it is characterized in that concocting method is as follows:

(1), get Radix Aconiti Lateralis Preparata, select stepping, in placing container, add water to and flood Radix Aconiti Lateralis Preparata fully, between soak period, change water 2-4 every day, when soaking water at 25 ℃, electrical conductivity is down to 4000 μ s/cm when following, takes out, drain the water step (1) make thing;

(2), by step (1) make thing expand the part upward, evenly be laid in the clean thin bran ash burnt that 20-30cm is thick, cover in the above the clean ginger slice of some layers, the thing that makes by every 100kg step (1) adds the 12kg Rhizoma Zingiberis Recens, on ginger slice, the flat 2-4 that covers opens toilet paper, repave the clean thin bran ash that one deck 5-8cm is thick on paper, the thick dry bran shell of tiling 10-15cm on ash;

(3), ignite at the dry bran shell four angle point fire of tiling, control the upper end temperature of Radix Aconiti Lateralis Preparata at 140-170 ℃, the lower end temperature is at 80-110 ℃, uninterruptedly simmers 36-48 hour processed, treats that the bran ash is cooling, and the taking-up Radix Aconiti Lateralis Preparata obtains the thing that makes of step (3);

(4), the thing that makes of step (3) is placed in heating container, uninterruptedly steam 8～10 hours, incline and, airing to room temperature obtains the thing that makes of step (4);

(5), get the thing that makes of step (4), cut the vertical thin slice that 2-3mm is thick, be dried to water content 6%～12% at 60～80 ℃, obtain.

Images