CN103462874B - Donkey-hide gelatin essence whitening mask and preparation method thereof - Google Patents
Donkey-hide gelatin essence whitening mask and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a donkey-hide gelatin essence whitening mask and a preparation method of the donkey-hide gelatin essence whitening mask. The mask is composed of the following raw materials of angelica dahurica, white tuckahoe, white atractylodes, batryticated silkworm, palmetto, allantoin, sodium hyalurate, glycerin, squalane, Lubrajel oil, self-emulsion monoglyceride, beeswax, carbomer and triethanolamine. The mass fraction of free amino acid of donkey-hide gelatin essence of the mask is 4%, the mass fraction of small peptide with molecular weight between 200 Da and 1000 Da is 80-85%, and the mass fraction of peptide segments with molecular weight between 1000 Da and 3000 Da is 14%. The PH of the donkey-hide gelatin essence whitening mask is 5.0-7.5. The donkey-hide gelatin essence whitening mask can whiten the skin, remove wrinkles and enhance elasticity of the skin, makes the skin fine, smooth and glossy, passes the anti-allergic test, has no any side effect, and is safe, reliable and capable of being used as mask skin care products for a long time.
Description
Technical field
The present invention relates to a kind of cleawhite pack and preparation method thereof, particularly a kind of donkey-hide gelatin essence cleawhite pack, belongs to Chinese medicine cosmetic field.
Background technology
With advancing age, the aggravation of environmental pollution, the young white collar enduring work weight to the fullest extent causes skin to occur problems because of reasons such as ultraviolet, dust, waste gas, computer radiates, add people to the continuous pursuit of U.S. and the demand to health, the beauty mask series products being primary efficacy with whitening, moisturizing, wrinkle removing, light speckle etc. obtains the favor of most consumers.Accordingly, we have invented that a kind of raw material is simple, definite ingredients, effect significantly, the facial film of technique simple possible.
Colla Corii Asini is panacea of fostering the spirit of nobility of enriching blood, and is just listed in " top grade ", and records " taking QI invigorating for a long time to make light of one's life by commiting suicide " in the famous medical works Shennong's Herbal of the Eastern Han Dynasty.The traditional Chinese medical science is said " woman take blood as this ", and QI and blood is vigorous then healthy, and appearance is handsome, and aging rate slows down.Have existed since ancient times for the function of Colla Corii Asini looks improving and the skin nourishing, one of historic " four great beauties " highest-ranking imperial concubine Yang, good and famous with skin.The Tang Dynasty famous poet Pai Chu-yi describes highest-ranking imperial concubine's Yang " skin is as lipoid " in its " song of everlasting sorrow ", secret in having said in another poet Xiao Hang of the Tang Dynasty " palace word addendum ": " lead powder used in cosmetics is washed to the greatest extent according to plentiful; the rain Folium Nelumbinis pearl difficulty that falls is stopped, and secretly taking Colla Corii Asini be unwilling to road, we were telling you be monarch's appearance from birth." Colla Corii Asini main component is collagen protein, effectively can pin the moisture in skin, and increase the elasticity of skin, suppresses the generation of wrinkle.Present published Colla Corii Asini class facial film patent (201110066348.7,98126247.3,200710034205.1,99117374.0) all adopts Colla Corii Asini to be face-mask material, but modern study mechanism is thought, in Colla Corii Asini, collagen molecules amount is larger, more difficultly directly to be absorbed by human body skin, greatly limit the performance of Colla Corii Asini beauty functions.
It is main face-mask material by the donkey-hide gelatin essence obtained after composite protease hydrolysis that the present invention adopts genuine medicinal materials Donga Colla Corii Asini, and through experimental results demonstrate, the present invention's more common Colla Corii Asini facial film assimilation effect is better.
Donkey-hide gelatin essence can supplement skin collagen; Hyaluronate sodium is recognized has very strong whitening skin and preserving moisture effect, and has certain wrinkle removing and increase skin elasticity effect; The Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus have remarkable whitening function, and are just commonly used for the product of whitening in history records are recorded; Be rich in necessary eight seed amino acids of human body in Cera Flava, effectively can promote the synthesis of skin collagen; Glycerol, squalane, Lubrajel Oil, self emulsifying monoglyceride, with optimal proportion proportioning, present best oil-water ratio; Carbomer rises through profit after dissolving, with triethanolamine adjusting viscosity and pH value, makes the acid-base value of facial mask liquid and oil-water ratio and skin corresponding proportion close, to reach best skin effect.The donkey-hide gelatin essence facial film be made up of above-mentioned raw materials is tried out through volunteer, and result display uses facial film of the present invention can Whitening, spot, and moisturizing wrinkle removing, increases skin elasticity, make skin fine and smooth, smooth, glossy.Meanwhile, facial film of the present invention is tested by antiallergic, without any side effects, safe and reliable, can be used as facial film class skin-protection product life-time service.
Summary of the invention
For the problems of the prior art, the invention provides a kind of donkey-hide gelatin essence cleawhite pack and preparation method thereof.
To achieve the above object, present invention employs following technical scheme:
A kind of donkey-hide gelatin essence cleawhite pack, is made up of the raw material of following parts by weight:
Donkey-hide gelatin essence 1-3 part, Radix Angelicae Dahuricae 70-120 part, Poria 70-120 part, Rhizoma Atractylodis Macrocephalae 70-120 part, Bombyx Batryticatus 70-120 part, palmetto 70-120 part, allantoin 1-3 part, hyaluronate sodium 15-25 part, glycerol 10-20 part, squalane 5-15 part, Lubrajel Oil 3-10 part, self emulsifying monoglyceride 3-8 part, Cera Flava 1-3 part, carbomer 0.05-0.15 part, triethanolamine 0.02-0.08 part.
Described donkey-hide gelatin essence Free Amino Acids mass fraction is 4%, and molecular weight is 80%-85% between the mass fraction of the little peptide of 200-1000Da, and the mass fraction between the peptide section of 1000-3000Da is 14%.
Described glue elite cleawhite pack pH is 5.0-7.5, and preferred pH is 5.8-6.5.
Preferred, be made up of the raw material of following parts by weight: the Radix Angelicae Dahuricae 100 parts, Poria 100 parts, the Rhizoma Atractylodis Macrocephalae 100 parts, Bombyx Batryticatus 100 parts, palmetto 100 parts, allantoin 2 parts, hyaluronate sodium 20 parts, glycerol 15 parts, squalane 10 parts, Lubrajel Oil 6 parts, self emulsifying monoglyceride 5 parts, 2 parts, Cera Flava, carbomer 0.1 part, triethanolamine 0.05 part.
Prepare the method for above-mentioned donkey-hide gelatin essence cleawhite pack, comprise the following steps:
1, the preparation of donkey-hide gelatin essence: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%,
2, join the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto in raw material gross weight 10 times amount pure water, ebuillition of heated extracts 1 hour; Filter, collect extracting solution, raw material slag adds 10 times amount pure water again, and ebuillition of heated extracts 1 hour, again filters, and discards raw material slag, collects filtrate.Mixing extracted twice liquid, when being evaporated to 20 DEG C, relative density is 1.20.Carbomer is dissolved in pure water, pH to 5.0-7.5 is regulated with triethanolamine, then allantoin, squalane, Lubrajel Oil and Cera Flava is added after adding extracting solution, donkey-hide gelatin essence, hyaluronate sodium, glycerol and self emulsifying monoglyceride mix homogeneously, be stirred into thick paste, under 20 DEG C of conditions, add pure water regulate the relative density of thick paste to be 1.05-1.10.
3, by the thick paste sterilizing of step 1 gained, subpackage, to obtain final product.
Preferably, described sterilizing adopts ultraviolet disinfection, and sterilization time is 30min-60min.
Donkey-hide gelatin essence is obtained after compound protease zymolysis by genuine traditional Chinese medicine material Donga Colla Corii Asini, Colla Corii Asini is panacea of fostering the spirit of nobility of enriching blood, just be listed in " top grade " in the famous medical works Shennong's Herbal of the Eastern Han Dynasty, and record " taking QI invigorating for a long time to make light of one's life by commiting suicide ".Colla Corii Asini is sweet, flat, and its function is mainly enriched blood YIN nourishing, moisturizes, stops blooding.The traditional Chinese medical science is said " woman take blood as this ", and QI and blood is vigorous then healthy, and appearance is handsome, and aging rate slows down.Pharmacological research confirms, Colla Corii Asini can also stimulate body cell to produce SOD(superoxide dismutase), and the activity of SOD can be improved, and SOD can scavenging activated oxygen, has the effect of looks improving and the skin nourishing, slow down aging.Meanwhile, Colla Corii Asini can generate by check melanin, obvious to skin-whitening effect.But collagen molecules amount is comparatively large in Colla Corii Asini, more difficultly directly to be absorbed by human body skin, greatly limit the performance of Colla Corii Asini beauty functions.Nearly decades, existing many domestic and foreign literature report (Adibi A S.America Nutrition, 1984,25:1114-1122; D.B.A.Silk.Gut, 1974,15:494-501) molecular weight occupy the absorption of peptide section between 200-1000Da and effect relative to the peptide section of other sizes and free amino acid all better.Donkey-hide gelatin essence Free Amino Acids mass fraction of the present invention is 4%, and molecular weight is 80%-85% between the mass fraction of the little peptide of 200-1000Da, and the mass fraction between the peptide section of 1000-3000Da is 14%, and mean molecule quantity is 765Da.Through bioavailability detection experiment, finding that the bioavailability of donkey-hide gelatin essence is 3.5 times of common Colla Corii Asini, is 2.2 times of Bionic digestion Colla Corii Asini.In this digital proof the present invention as the donkey-hide gelatin essence of facial film raw material relative to common Colla Corii Asini, have that significant molecule is little, the feature of high-selenium corn, can the effect of more effective performance scavenging free radicals and supplementary skin collagen.
The present invention selects donkey-hide gelatin essence, the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto, allantoin, hyaluronate sodium, glycerol, squalane, Lubrajel Oil, self emulsifying monoglyceride, Cera Flava, carbomer as the raw material of donkey-hide gelatin essence facial film.Mentioned component, by reasonably combined, have the significant effect supplementing skin collagen, Whitening, spot, moisturizing wrinkle removing, increase skin elasticity, adheres to using this facial film can effectively improve skin of face situation, reach beauty treatment, keeps the object of skin youth.
Beneficial effect of the present invention: can whitening wrinkle removing, increases skin elasticity, makes skin fine and smooth, smooth, glossy, without any side effects, safe and reliable, can be used as facial film class skin-protection product life-time service.
Compared with prior art, the advantage of patent of the present invention:
1, facial film main component donkey-hide gelatin essence of the present invention is the deep processed product of Colla Corii Asini, and molecule is less, absorbs effect of better Colla Corii Asini nourishing blood skin care through history checking in thousand.
2, facial film of the present invention mostly is Chinese crude drug extract component, more natural, safer.
Accompanying drawing explanation
Fig. 1 is the mixture graph of molecular weight distribution of the donkey-hide gelatin essence adopting high productivity computing combine with technique Analytical Technology of Amino Acid to determine;
Fig. 2 is for get blood by mouse stomach, and in detection blood, hydroxyproline content is with the comparison diagram of the product bioavailability determined.
Detailed description of the invention
Further describe the present invention below in conjunction with specific embodiment, advantage and disadvantage of the present invention will be more clear along with description.But embodiment is only exemplary, does not form any restriction to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments and replacement all fall within the scope of protection of the present invention.
Common Colla Corii Asini: i.e. undressed common Colla Corii Asini sample;
Bionic digestion Colla Corii Asini: imitate human gastrointestinal tract digestive system, adopts pepsin and intestinal pancreatin enzymolysis Colla Corii Asini;
Donkey-hide gelatin essence: namely Colla Corii Asini is by the less mixture of the molecular weight that obtains after the zymolysis of compound protease;
Embodiment 1 one kinds of donkey-hide gelatin essence cleawhite pack and preparation method thereof
A kind of donkey-hide gelatin essence cleawhite pack, is made up of the raw material of following weight portion:
Donkey-hide gelatin essence 2 parts, cucumber extract 1 part, Punica granatum L. extract 1 part, Sargassum extract 2 parts, palmetto extracting solution 2 parts, glycyrrhizic acid dipotassium 5 parts, Lubrajel Oil 5 parts, glycerol 1.5 parts, 1,3 butylene glycol 1 part, allantoin 2 parts, hyaluronate sodium 2 parts, carbomer 0.1 part, triethanolamine 0.05 part.
Prepare the method for above-mentioned donkey-hide gelatin essence cleawhite pack, comprise the following steps:
1, the preparation of donkey-hide gelatin essence: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%,
2, join the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto in raw material gross weight 10 times amount pure water, ebuillition of heated extracts 1 hour; Filter, collect extracting solution, raw material slag adds 10 times amount pure water again, and ebuillition of heated extracts 1 hour, again filters, and discards raw material slag, collects filtrate.Mixing extracted twice liquid, when being evaporated to 20 DEG C, relative density is 1.20.Carbomer is dissolved in pure water, pH to 6.0 is regulated with triethanolamine, then allantoin, squalane, Lubrajel Oil and Cera Flava is added after adding extracting solution, donkey-hide gelatin essence, hyaluronate sodium, glycerol and self emulsifying monoglyceride mix homogeneously, be stirred into thick paste, under 20 DEG C of conditions, add pure water regulate the relative density of thick paste to be 1.05.
3, by thick paste sterilizing ultraviolet disinfection 40min, the subpackage of step 2 gained, to obtain final product.
Embodiment 2 one kinds of donkey-hide gelatin essence cleawhite pack and preparation method thereof
A kind of donkey-hide gelatin essence cleawhite pack, is made up of the raw material of following weight portion:
Donkey-hide gelatin essence 1 part, the Radix Angelicae Dahuricae 75 parts, Poria 75 parts, the Rhizoma Atractylodis Macrocephalae 75 parts, Bombyx Batryticatus 75 parts, palmetto 75 parts, allantoin 1 part, hyaluronate sodium 16 parts, glycerol 12 parts, squalane 5 parts, Lubrajel Oil 4 parts, self emulsifying monoglyceride 3 parts, 1 part, Cera Flava, carbomer 0.06 part, triethanolamine 0.03 part.
Prepare the method for above-mentioned donkey-hide gelatin essence cleawhite pack, comprise the following steps:
1, the preparation of donkey-hide gelatin essence: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%,
2, join the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto in raw material gross weight 10 times amount pure water, ebuillition of heated extracts 1 hour; Filter, collect extracting solution, raw material slag adds 10 times amount pure water again, and ebuillition of heated extracts 1 hour, again filters, and discards raw material slag, collects filtrate.Mixing extracted twice liquid, when being evaporated to 20 DEG C, relative density is 1.20.Carbomer is dissolved in pure water, pH to 5.8 is regulated with triethanolamine, then allantoin, squalane, Lubrajel Oil and Cera Flava is added after adding extract, donkey-hide gelatin essence, hyaluronate sodium, glycerol and self emulsifying monoglyceride mix homogeneously, be stirred into thick paste, under 20 DEG C of conditions, add pure water regulate the relative density of thick paste to be 1.08.
3, by thick paste sterilizing ultraviolet disinfection 30min, the subpackage of step 2 gained, to obtain final product.
Embodiment 3 one kinds of donkey-hide gelatin essence cleawhite pack and preparation method thereof
A kind of donkey-hide gelatin essence cleawhite pack, is made up of the raw material of following weight portion:
Donkey-hide gelatin essence 3 parts, the Radix Angelicae Dahuricae 110 parts, Poria 110 parts, the Rhizoma Atractylodis Macrocephalae 110 parts, Bombyx Batryticatus 110 parts, palmetto 110 parts, allantoin 3 parts, hyaluronate sodium 23 parts, glycerol 20 parts, squalane 15 parts, Lubrajel Oil 10 parts, self emulsifying monoglyceride 7 parts, 3 parts, Cera Flava, carbomer 0.15 part, triethanolamine 0.07 part.
Prepare the method for above-mentioned donkey-hide gelatin essence cleawhite pack, comprise the following steps:
1, the preparation of donkey-hide gelatin essence: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%,
2, join the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto in raw material gross weight 10 times amount pure water, ebuillition of heated extracts 1 hour; Filter, collect extracting solution, raw material slag adds 10 times amount pure water again, and ebuillition of heated extracts 1 hour, again filters, and discards raw material slag, collects filtrate.Mixing extracted twice liquid, when being evaporated to 20 DEG C, relative density is 1.20.Carbomer is dissolved in pure water, pH to 6.5 is regulated with triethanolamine, then allantoin, squalane, Lubrajel Oil and Cera Flava is added after adding extract, donkey-hide gelatin essence, hyaluronate sodium, glycerol and self emulsifying monoglyceride mix homogeneously, be stirred into thick paste, under 20 DEG C of conditions, add pure water regulate the relative density of thick paste to be 1.10.
3, by thick paste sterilizing ultraviolet disinfection 60min, the subpackage of step 2 gained, to obtain final product.
Donkey-hide gelatin essence in embodiment 1 is carried out following experimental analysis: (wherein (application number 201110450564.1 is mentioned at " mixture of a kind of active small-molecule donkey-hide gelatin and its preparation method and application " for the experimentation of embodiment 4 and embodiment 5 and result, these two embodiments do not belong to the novelty of this patent, only for the performance of micromolecule Colla Corii Asini raw material and the advantage relative to common Colla Corii Asini are described, the novelty adopting micromolecule Colla Corii Asini to carry out facial film exploitation for this patent provides foundation)
Active small molecular peptide section distribution tests in test example 1 Colla Corii Asini mixture of the present invention
1.1 high productivity computing tests
Test instrunment:
Liquid phase systems: Agilent1100; High productivity computing post: TSK-G2000SKwl(300mm*7.8mm, average pore size
).
Testing procedure:
Get sample after 1ml enzymolysis, centrifugal 2 minutes of 10,000g, get supernatant and filtered by 0.45 μm of disposable filter, filtrate adds in sample injection bottle.Column temperature is 27 DEG C, and mobile phase is 0.05mol/L phosphate buffer (pH7.2), and flow velocity is 0.5ml/min, determined wavelength 280nm, sampling volume 20 μ l.
1.2 free aminoacid content tests
Test instrunment:
Mass spectrograph: 3200Q Trap; Liquid phase systems: Shimadzu UFLC system; Analytical column: aminoacid dedicated columns, AB Sceix; Guard column: C18(4.0 × 3.0mm, 5 μm) Phenomenex company.
Testing procedure:
Get the fluid sample after 20 μ L mixings, add 5 μ L precipitant, mixing, centrifugal 2 minutes of 10,000g, Aspirate supernatant 5 μ L adds 20 μ L labelling buffer, mixing, gets supernatant 5 μ L and adds 2.5 μ L labelled reagents, mixing, room temperature reaction 30min, adds 2.5 μ L azanols after reaction 30min, mixing, 40 DEG C of nitrogen dry up, and add mark liquid restructuring in 16 μ L, mixing, draw 16 μ L in sample injection bottle, sample introduction 5 μ L surveys amino acid concentration.
1.3 test result
As shown in Figure 1, as can be seen from Figure 1, molecular weight is very high between the little peptide content of 200-1000Da, reaches 81%, and the peptide section between 1000-3000Da accounts for 14%, and free aminoacid content is very low, only has 4%, and mean molecule quantity is 765Da for result of the test.Described in background: molecular weight occupy the absorption of peptide section between 200-1000Da and effect relative to the peptide section of other sizes and free amino acid all better.Can think, the present invention relatively other collagen peptide products has absorption rate and better functional effect faster.
Test example 2 bioavailability is tested
Test material
Common Colla Corii Asini: i.e. undressed common Colla Corii Asini sample;
Bionic digestion Colla Corii Asini: imitate human gastrointestinal tract digestive system, adopts pepsin and intestinal pancreatin enzymolysis Colla Corii Asini;
Micromolecule Colla Corii Asini: i.e. active small molecular in Colla Corii Asini mixture of the present invention;
Get healthy mice 24, male and female half and half, between body weight 200-250g, be divided into A group, B group and C group at random.Fasting 12h before taking medicine, morning next day A group, B group and component C gavage Colla Corii Asini, Bionic digestion Colla Corii Asini and micromolecule Colla Corii Asini (1mg sample/10g body weight) not on an empty stomach, unified feed after administration 4h, blood is got, by hydroxyproline content in process employing amino acid analysis method analysis blood in order to calibration sample bioavailability (hydroxyproline is the amino acids characteristic of collagen protein) in administration 0h, 0.25h, 0.5h, 1h, 2h, 3h, 4h, 6h, 8h, 10h, 12h, 18h, 24h eye socket.
As shown in Figure 2, as can be seen from Figure 2, in bioavailability, the little peptide mixer of Colla Corii Asini collagen of the present invention is 3.5 times of common Colla Corii Asini to result, is 2.2 times of Bionic digestion Colla Corii Asini.This digital proof active small-molecule donkey-hide gelatin mixture of the present invention has that molecule is little, the feature of high-selenium corn.
Test example 3 donkey-hide gelatin essence cleawhite pack of the present invention beauty functions
Trier is age-based, skin quality, and professional degree is divided into three groups, and all experimenters are more than 18 years old, the healthy women trier participated in voluntarily.Wherein 20-35 year for lineup's number be that 10 people organize into groups A, within 35 years old to 55 years old, be that one group of 10 people organizes into groups B, another group organizes into groups C for professional person 10 people.Micromolecule Colla Corii Asini facial film is measured the bimester that every trier distributing respectively.Occupation mode is: spread on the face after cleaning, is removed by remaining facial film after 20 minutes with clear water, once a day, continues two months (60 days) on probation.
Proof test result is as follows:
The anaphylaxis of skin and zest: tested all women do not have irritated reflection, and do not have skin irritation to reflect in tested period, and wherein the professional person experimenter of C group unanimously thinks that product gentleness is non-stimulated, high safety.
Beautifying skin effect, specifically comprises subjective use sense, moisture retention, moistness, whitening, and the result after the bimester that 30 volunteers trying out gathers as follows:
The all volunteers of table 1 micromolecule Colla Corii Asini facial film try out efficacy outcomes
| Project | Effective (%) | Effect general (%) | Effect bad (%) |
| Subjective use sense | 93％ | 7％ | 0％ |
| Heat insulating ability | 90％ | 10％ | 0％ |
| Moistness | 82％ | 18％ | 0％ |
| Whitening | 91％ | 9％ | 0％ |
Claims (6)
1. a donkey-hide gelatin essence cleawhite pack, is characterized in that, is made up of the raw material of following parts by weight:
Donkey-hide gelatin essence 1-3 part, Radix Angelicae Dahuricae 70-120 part, Poria 70-120 part, Rhizoma Atractylodis Macrocephalae 70-120 part, Bombyx Batryticatus 70-120 part, palmetto 70-120 part, allantoin 1-3 part, hyaluronate sodium 15-25 part, glycerol 10-20 part, squalane 5-15 part, Lubrajel Oil 3-10 part, self emulsifying monoglyceride 3-8 part, Cera Flava 1-3 part, carbomer 0.05-0.15 part, triethanolamine 0.02-0.08 part;
Wherein, described donkey-hide gelatin essence prepares by the following method: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%, mean molecule quantity is 765Da.
2. donkey-hide gelatin essence cleawhite pack according to claim 1, it is characterized in that, be made up of the raw material of following parts by weight: the Radix Angelicae Dahuricae 100 parts, Poria 100 parts, the Rhizoma Atractylodis Macrocephalae 100 parts, Bombyx Batryticatus 100 parts, palmetto 100 parts, allantoin 2 parts, hyaluronate sodium 20 parts, glycerol 15 parts, squalane 10 parts, Lubrajel Oil 6 parts, self emulsifying monoglyceride 5 parts, 2 parts, Cera Flava, carbomer 0.1 part, triethanolamine 0.05 part.
3. donkey-hide gelatin essence cleawhite pack according to claim 1, is characterized in that, described pH is 5.8-6.5.
4. prepare the method for the donkey-hide gelatin essence cleawhite pack described in any one of claim 1-3, comprise the following steps:
(1) preparation of donkey-hide gelatin essence: get mass concentration be 20% Colla Corii Asini glue juice be enzymolysis raw material, the ratio being 100:1 in the mass ratio of Colla Corii Asini glue juice and compound protease adds compound protease, wherein in compound protease, the mass ratio of papain and proline protein enzyme is 2:1, enzymolysis 2 hours, enzymolysis solution obtains donkey-hide gelatin essence solution through membrane filtration, spray-dried obtained donkey-hide gelatin essence mixture, wherein, free amino acid mass fraction is 4%, molecular weight between the mass fraction of the little peptide of 200-1000Da be 80%-85%, mass fraction between the peptide section of 1000-3000Da is 14%, mean molecule quantity is 765Da,
(2) join the Radix Angelicae Dahuricae, Poria, the Rhizoma Atractylodis Macrocephalae, Bombyx Batryticatus, palmetto in raw material gross weight 10 times amount pure water, ebuillition of heated extracts 1 hour; Filter, collect extracting solution, raw material slag adds 10 times amount pure water again, and ebuillition of heated extracts 1 hour, again filters, and discards raw material slag, collects filtrate, mixing extracted twice liquid, and when being evaporated to 20 DEG C, relative density is 1.20; Carbomer is dissolved in pure water, pH to 5.0-7.5 is regulated with triethanolamine, then allantoin, squalane, Lubrajel Oil and Cera Flava is added after adding extracting solution, donkey-hide gelatin essence, hyaluronate sodium, glycerol and self emulsifying monoglyceride mix homogeneously, be stirred into thick paste, under 20 DEG C of conditions, add pure water regulate the relative density of thick paste to be 1.05-1.10;
(3) by the thick paste sterilizing of step (2) gained, subpackage, to obtain final product.
5. method according to claim 4, is characterized in that, described thick paste relative density under 20 DEG C of conditions is 1.08.
6. method according to claim 4, is characterized in that, described sterilizing adopts ultraviolet disinfection, and sterilization time is 30min-60min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310452986.1A CN103462874B (en) | 2013-09-24 | 2013-09-24 | Donkey-hide gelatin essence whitening mask and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310452986.1A CN103462874B (en) | 2013-09-24 | 2013-09-24 | Donkey-hide gelatin essence whitening mask and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103462874A CN103462874A (en) | 2013-12-25 |
| CN103462874B true CN103462874B (en) | 2015-07-08 |
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| CN107343872A (en) * | 2017-09-08 | 2017-11-14 | 呙如松 | A kind of face mask of traditional Chinese medicine and preparation method thereof |
| CN109846756A (en) * | 2019-02-01 | 2019-06-07 | 重庆工业职业技术学院 | A kind of moisturizing emulsion of the extract containing beeswax and preparation method thereof |
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| CN101664373A (en) * | 2008-09-03 | 2010-03-10 | 张宁 | Donkey milk cosmetic and preparation method thereof |
| CN102133168A (en) * | 2011-03-18 | 2011-07-27 | 汤广武 | Novel beautifying mask and preparation method thereof |
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| CN101664373A (en) * | 2008-09-03 | 2010-03-10 | 张宁 | Donkey milk cosmetic and preparation method thereof |
| CN102133168A (en) * | 2011-03-18 | 2011-07-27 | 汤广武 | Novel beautifying mask and preparation method thereof |
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Inventor after: Qin Yufeng Inventor after: You Jinhua Inventor after: Zhou Xiangshan Inventor after: Han Li Inventor after: Tian Shousheng Inventor after: Feng Mingjian Inventor after: Liu Fei Inventor after: Fang Zhe Inventor before: Qin Yufeng Inventor before: You Jinhua Inventor before: Zhou Xiangshan Inventor before: Han Li Inventor before: Tian Shousheng Inventor before: Feng Mingjian Inventor before: Liu Fei Inventor before: Fang Zhe Inventor before: Shi Zhaosong |
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