CN103460921A - Emmenopterys henryi pollution-free planting method - Google Patents
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Abstract
The invention discloses an emmenopterys henryi pollution-free planting method which comprises the steps of selection of seeds, disinfection of the seeds, centrifugal overweight treatment, plant hormones and low temperature sand stratification treatment, seed germination and soil scientific management, seedling transplanting and the like. By the centrifugal overweight treatment and the plant hormones and low temperature sand stratification treatment, sleep time of the seeds of emmenopterys henryi is shortened; moreover, by effective temperature and light treatment, the emmenopterys henryi has high seed germination rate, germinability and germination index.
Description
Technical field
The invention belongs to the plant cultivation field, be specifically related to a kind of method of Emmenopterys henryi non-polluted planting.
Background technology
Emmenopterys henryi (Emmenopterys Henryi Oliv.) has another name called fourth wood, the great Ye water seeds of a tung oil tree, little wax gourd, eggplant tree etc., for the Rubiaceae Emmenopterys henryi belongs to seeds [1].It is the ancient Relict Plant of China, originates from apart from the modern approximately Cretaceous period in Mesozoic Era of 100,000,000 years, is listed in national II level protective plant.Mainly be distributed in the deciduous broadleaved forest or evergreen, Deciduous Broad Leaved Mixed Forest of mountain in the subtropics such as Anhui, Zhejiang, Jiangxi, Jiangsu or low mountainous region.The root of Emmenopterys henryi, bark can be used as medicine, and in wet, with stomach, stopping nausea and vomiting by lowering the adverse flow of QI, gastric disorder causing nausea, vomiting, hiccup etc., having certain curative effect, have important pharmaceutical value.Emmenopterys henryi is the peculiar autogenus plant of China, and the floristic region system of studying Rubiaceae systematic growth and south China, the west and south is all had the certain significance; In addition, its timber can be used material as building, furniture etc., and the tree performance grace is spent greatly and gorgeously, is again good ornamental plants.Therefore strengthen that the research of breeding of Emmenopterys henryi is had to important economic worth and ecological significance
Although the Emmenopterys henryi distribution is wider, how scattered growth, and because deforestation is opened up wasteland and indiscriminate felling of trees, add that himself seed germination power is lower, natural regeneration ability, thereby natural distributed phases down, and plant reduces day by day, and large tree, veteran are rare especially.Therefore, strengthen that the research of Emmenopterys henryi propagation technique is had to Important Economic and the ecological value.The process of Emmenopterys henryi field seminal propagation must have enough provenance numbers, otherwise, under the condition that does not have to form at forest community, the population quantity that is difficult to reach certain realizes minimum viable population, so its population will be the trend of decline and extinction.And the germination rate of seed is lower, there is no scientific soil management pattern in the wild; Therefore restricted to a great extent the breeding of Emmenopterys henryi.
Summary of the invention
The technical problem that the present invention mainly solves is to utilize the artificial technology to abolish the Emmenopterys henryi seed dormancy, and takes certain measure to improve the seed germination rate of Emmenopterys henryi.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 3-4 hour, then use 10 times of solution seed soaking 3-4 hour of 40% sulfuric acid trisodium, then filters and removes described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing;
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 10-50 μ L/L salicylic acid mixed solution of 20-100 μ L/L, soak time is with the wet sand of moisture 10-15%, to mix after 2-4 h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.1%ABA and 0.5%GA, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 22-25 ℃ of greenhouse and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1500-1700LX, and light application time is 13 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) soil environment is cultivated: regulating the soil pH value is 7.0～8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, wherein pine needle, original soil, muck, Cosan are according to ratio of weight and number 2:3:1:2, perhaps in soil, apply saw not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the ratio of weight and number of the peat composed of rotten mosses, liver moss and Cosan is 1:1:1:2;
Increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%～5%, to increase the permeability of soil;
Soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5～10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil in step (6), keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 15-25 days, grow the seedling of 10-20 centimetre.
The invention has the beneficial effects as follows:
(1) can abolish kind of a skin obstacle through suitable centrifugal overweight processing, be conducive to breaking dormancy.
(2) by the effective breaking dormancy of gibberellin and salicylic acid mixed processing, and adopt first 4 ℃ of low temperature in low temperature Sand culture process, 25 ℃ of constant temperature and then 4 ℃ of low temperature treatment can help the quick breaking dormancy of seed again, and add suitable HORMONE TREATMENT at each temperature treatment phase, can promote the fast-germination of seed.
(3) in the seed germination stage, take suitable temperature and light treatment, germination rate, germination vigor and germination index all are improved.
(4) adopt the soil management pattern of science, improved soil fertility, and the Pest management rate.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
embodiment 1
A kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2 times with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 3 hours, then use 10 times of solution seed soakings of 40% sulfuric acid trisodium 3 hours, then filters and removes described thimerosal; Use again the described seed of sterile water wash 2 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing; The rotating speed of high speed freezing centrifuge is 3000r/min, and the centrifugal overweight processing time is 1h.
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 50 μ L/L salicylic acid mixed solution of 20 μ L/L, soak time is with moisture 10% wet sand, to mix after 2h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.1%ABA and 0.5%GA, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 22 ℃ of greenhouses and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1500LX, and light application time is 13 hours/day, and temperature is 23 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) transplanting of seedling: transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 12 cm thicks by volume for detritus soil: vermiculite: rice chaff: garden mould=3: 2: 1: 1 mixture, the perlite that lower floor is 5 cm thicks and river sand, keep 25 ℃ of left and right of temperature, relative moisture 70%, after 15-25 days, grow the seedling of 10-20 centimetre; The ratio of wherein said perlite and river sand is 1:1.
Result shows: the seed germination rate of Emmenopterys henryi is 94%, and germination vigor is 92%, and germination index is 54.5.
embodiment 2
A kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 3 times with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 4 hours, then use 10 times of solution seed soakings of 40% sulfuric acid trisodium 4 hours, then filters and removes described thimerosal; Use again the described seed of sterile water wash 3 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing; The rotating speed of high speed freezing centrifuge is 3500r/min, and the centrifugal overweight processing time is 2h.
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 10 μ L/L salicylic acid mixed solution of 100 μ L/L, soak time is with moisture 15% wet sand, to mix after 4 h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.1%ABA and 0.5%GA, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 25 ℃ of greenhouses and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1700LX, and light application time is 13 hours/day, and temperature is 25 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) transplanting of seedling: transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 15 cm thicks by volume for detritus soil: vermiculite: rice chaff: garden mould=3: 2: 1: 1 mixture, the perlite that lower floor is 7 cm thicks and river sand, keep 25 ℃ of left and right of temperature, relative moisture 75%, after 15-25 days, grow the seedling of 10-20 centimetre; The ratio of wherein said perlite and river sand is 2:1.
Result shows: the seed germination rate of Emmenopterys henryi is 95%, and germination vigor is 93%, and germination index is 52.5.
embodiment 3
A kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 3 times with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 3 hours, then use 10 times of solution seed soakings of 40% sulfuric acid trisodium 4 hours, then filters and removes described thimerosal; Use again the described seed of sterile water wash 3 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing; The rotating speed of high speed freezing centrifuge is 3200r/min, and the centrifugal overweight processing time is 1.5h.
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 30 μ L/L salicylic acid mixed solution of 50 μ L/L, soak time is with moisture 12% wet sand, to mix after 3 h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.1%ABA and 0.5%GA, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 24 ℃ of greenhouses and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1600LX, and light application time is 13 hours/day, and temperature is 24 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) transplanting of seedling: transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 14 cm thicks by volume for detritus soil: vermiculite: rice chaff: garden mould=3: 2: 1: 1 mixture, the perlite that lower floor is 6 cm thicks and river sand, keep 25 ℃ of left and right of temperature, relative moisture 72%, after 15-25 days, grow the seedling of 10-20 centimetre; The ratio of wherein said perlite and river sand is 1:1.
Result shows: the seed germination rate of Emmenopterys henryi is 96%, and germination vigor is 94%, and germination index is 56.2.
Embodiment 4:
A kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 3-4 hour, then use 10 times of solution seed soaking 3-4 hour of 40% sulfuric acid trisodium, then filters and removes described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing;
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 10-50 μ L/L salicylic acid mixed solution of 20-100 μ L/L, soak time is with the wet sand of moisture 10-15%, to mix after 2-4 h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.1%ABA and 0.5%GA, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 22-25 ℃ of greenhouse and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1500-1700LX, and light application time is 13 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) soil environment is cultivated: regulating the soil pH value is 7.0～8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, wherein pine needle, original soil, muck, Cosan are according to ratio of weight and number 2:3:1:2, perhaps in soil, apply saw not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the ratio of weight and number of the peat composed of rotten mosses, liver moss and Cosan is 1:1:1:2;
Increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%～5%, to increase the permeability of soil;
Soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5～10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil in step (6), keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 15-25 days, grow the seedling of 10-20 centimetre.
The seed germination rate of Emmenopterys henryi is 98%, and germination vigor is 99%; And plant strain growth is vigorous, soil fertility 13.2%, disease control rate 22% have been improved.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present invention.
Claims (3)
1.
a kind of method of Emmenopterys henryi non-polluted planting comprises the following steps:
(1) choosing of seed: at first select the seed without damage by disease and insect of suitable locality, the seed newly gathered is selected to full grains, glossiness standby;
(2) sterilization of seed: seed cleans 2-3 time with warm water, then puts into 500 times of liquid of thiophanate methyl and soaks 3-4 hour, then use 10 times of solution seed soaking 3-4 hour of 40% sulfuric acid trisodium, then filters and removes described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) centrifugal overweight processing: will put into high speed freezing centrifuge through above-mentioned seed of disinfecting and carry out overweight processing;
(4) plant hormone and low temperature Sand culture: the Emmenopterys henryi seed that will filter out through disinfecting is soaked in the gibberellin and 10-50 μ L/L salicylic acid mixed solution of 20-100 μ L/L, soak time is with the wet sand of moisture 10-15%, to mix after 2-4 h again, be placed on husky the Tibetan 30 days in 4 ℃ of refrigerators, every 3 days, stir 1 time, keep seed moistening, then take out and screen out sand, thin layer dries in the shade, soak 24h with 0.1%ABA and 0.5%GA again, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, soak 12h with 0.4%ABA, then hide 40 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, bottom spreads double-deck filter paper, silk liquid is cultivated to be positioned in 22-25 ℃ of greenhouse and is carried out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep filter paper moistening, to reduce flow of water change as far as possible; Controlled light intensity is 1500-1700LX, and light application time is 13 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling;
(6) soil environment is cultivated: regulating the soil pH value is 7.0～8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, wherein pine needle, original soil, muck, Cosan are according to ratio of weight and number 2:3:1:2, perhaps in soil, apply saw not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the ratio of weight and number of the peat composed of rotten mosses, liver moss and Cosan is 1:1:1:2;
increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%～5%, to increase the permeability of soil;
soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5～10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil in step (6), keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 15-25 days, grow the seedling of 10-20 centimetre.
2.
the method of a kind of Emmenopterys henryi non-polluted planting according to claim 1, the rotating speed of the high speed freezing centrifuge described in step (3) is 3000-3500r/min, the centrifugal overweight processing time is 1-2h.
3.
the method of a kind of Emmenopterys henryi non-polluted planting according to claim 1, the ratio of the perlite described in step (6) and river sand is 1:1 or 2:1.
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