CN103449696B - Method for improving oil sludge separating and processing efficiency and bacterial strain used for method - Google Patents
Method for improving oil sludge separating and processing efficiency and bacterial strain used for method Download PDFInfo
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- CN103449696B CN103449696B CN201210181272.7A CN201210181272A CN103449696B CN 103449696 B CN103449696 B CN 103449696B CN 201210181272 A CN201210181272 A CN 201210181272A CN 103449696 B CN103449696 B CN 103449696B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
Abstract
The invention belongs to the environment-friendly field, and discloses a method for improving oil sludge separating and processing efficiency and a bacterial strain used for the method. The method for improving the oil sludge separating and processing efficiency comprises the following steps: (1), sampling and analyzing processed oil sludge; (2), determining microorganism nutrients to be added according to components and content of a nitrogen element and a phosphor element in the processed oil sludge; (3), adding a mixed culture of candida lipolytica Y-57 and pseudomonas putida P-101 and the microorganism nutrients into the to-be-processed oil sludge, stirring, ventilating and culturing for over 8 hours under the condition with pH of 6-10 under a temperature of 8 DEG C-45 DEG C to separate oil and mud in the oil sludge. According to the invention, a microorganism treatment method is adopted for separating the oil and the mud in the oil sludge to achieve an effect of an oil resource recovery rate; moreover, a chemical agent which generates influence to the environment is not added, so that secondary pollution is not generated. Resourceful treatment is carried out on oil-containing mud to obtain a certain economic benefit, and therefore, the method is an economical and effective oil sludge treating technology.
Description
Technical field
The invention belongs to field of Environment Protection, relate to and a kind ofly improve the method for greasy filth separation treatment efficiency and the bacterial strain of use thereof.
Background technology
In oil production, accumulating and the refining course of processing, the greasy filth that normal some oleaginousness of generation are higher, if these greasy filth are stacked arbitrarily, will become the important source of pollution in oil field and surrounding enviroment.Oil is difficult to after entering soil remove, both spoiled soil structures, again to microorganism and the edaphophyte ecosystem generation harm of soil self.
The composition of oily sludge is extremely complicated, generally be made up of oil-in-water, water-in-oil and suspended solid impurities, containing a large amount of aged crude oil, wax, bituminous matter, colloid, suspended solid, bacterium, salt, sour gas, corrosion product etc., also comprise the water conditioner such as a large amount of flocculation agent, inhibiter, Scale inhibitors, sterilant added in production process, the method process oily sludges such as the burning method in succession occurred in recent years, thermal desorption method, pyrogenic process, solidification method, because the reasons such as cost is high, crude oil cannot reclaim, secondary pollution all fail to promote on a large scale.The direct landfill of oily sludge or the rear landfill of solidification are the main method that current most oil field at home adopts, but the shortcoming of this method pollutes easily to be transferred, likely polluted underground water etc., both wasted the valuable energy wherein, and also likely caused environmental pollution; Burning method has and subtracts appearance Be very effective, processes safer, eliminates the advantage of pathogenic bacteria, but burn and must carry out in the special incinerator set up, energy consumption is high, facility investment is large, process specifications is higher, and needs to add ignition dope, there is the secondary pollution such as dust, waste gas.According to statistics, burn oily sludge per ton and need oil consumption 18.5kg, its processing cost is high, causes energy dissipation simultaneously therefore is also implemented.Chemical method is suitable for the oily sludge of oleaginousness more than 5 ~ 10%, because its treating processes needs to add chemical agent usually, need special treatment facility, complex disposal process, crude oil recovery is thorough, there is the problem such as waste residue and secondary waste water pollutent and does not obtain popularity use.Bioremediation Technology also becomes the focus of China's research at present.Great majority are all devoted to the high-effective microorganism studying labile organic compound, make great efforts the high efficient petroleum degrading bacteria kind that exploitation is more new.Because biological treating accelerates natural biology degradation rate by the optimization to environmental factors, be a kind of economy, the cleaning technique that can bear of efficient and ecology.Therefore, the research field of bioremediation Technology has expanded to the improvement to underground water, soil, pollution of the sea such as solid waste, agricultural chemicals, oil at present.
Microorganism grows for carbon source with the crude oil in oily sludge, and the tensio-active agent simultaneously produced can cut oil, makes microorganism more effectively degraded oil, thus reach oil in greasy filth, water, silt is separated and the object of contaminant degradation.The factor affecting oily sludge biodegrading is many and complicated, and in the kind of oil degradation microorganism, oily sludge, the kind of oil, component, physico-chemical property and residing state etc. are most important to the degraded of oily sludge.Therefore according to the greasy filth bioremediation of the oil degradation bacterial classification of certain specific place screening, foundation sometimes and be not suitable for the separating treatment of a ground greasy filth.
Summary of the invention
The object of this invention is to provide a kind of method improving greasy filth separation treatment efficiency.
Another object of the present invention is to provide the bacterial strain used in the method.
Object of the present invention realizes by following technical scheme:
Improve a method for greasy filth separation treatment efficiency, comprise following steps:
(1) to process greasy filth sampling analysis;
(2) determine according to nitrogen element and phosphoric composition and content in process greasy filth the micro-nutrients added;
(3) add by the dosage of 0.01%-1wt% in pending greasy filth described in the mixed culture of deposit number to be the Candida lipolytica Y-57 of CGMCC No.5790 and deposit number the be pseudomonas putida P-101 of CGMCC No.5801 and micro-nutrients and/or tensio-active agent, at PH=6-10, temperature 8-45 DEG C, 50-150rpm stirs, ventilates, cultivate more than 8 hours, the oil in greasy filth is separated with mud.
Wherein, described carries out sampling analysis to process greasy filth, comprise and analysis of physical and chemical property and/or endogenous microbes analysis are carried out to greasy filth sample, determine oil, mud accounting in greasy filth, obtain nitrogen element and phosphoric composition and content in local water, and recover the oil and/or add in oil refining process some affect the chemical agent of microorganism growth, the quantity of aerobic-anaerobic microbe flora.
When total nitrogen concentration is less than 200mg/L in described oil-sludge treatment system, adds nitrogenous source and be at least 200mg/L to total nitrogen concentration; When total phosphorus concentration is less than 25mg/L in oil-sludge treatment system, adds phosphorus source and be at least 25mg/L to total phosphorus concentration.
Described nitrogenous source micro-nutrients is ammonium chloride or urea, described phosphorus source micro-nutrients is phosphoric acid salt, and described tensio-active agent is selected from one or more of tween, sapn, rhamnolipid, alkylaryl polyglycol ether, alkyl polyglycol ether or alkylaryl sulphonate.
In described Candida lipolytica Y-57 and the mixed culture of pseudomonas putida P-101, the quantity of effective microbe is greater than 10
5cfu/mL.
Described Candida lipolytica Y-57 and the mixed culture of pseudomonas putida P-101 are prepared by the following method: Candida lipolytica Y-57 and pseudomonas putida P-101 is inoculated in fermention medium, at 30 DEG C, stir 100-300rpm, air quantity 1:0.4-0.5, cultivates 16-48 hour under tank pressure 0.05-1.0mpa.
Described fermentative medium formula is: glucose 5-20 grams per liter, whiteruss 10-30 grams per liter, yeast extract paste 1-5.0 grams per liter, magnesium sulfate 0.1-0.5 grams per liter, Repone K 0.1-5.0 grams per liter, potassium primary phosphate 1-5.0 grams per liter, urea 0.5-5.0 grams per liter, ammonium nitrate 0.5-5.0 grams per liter, tween-80 0.1-0.5 milliliter/liter, Trace salts solution 1-3.0 milliliter/liter, pH nature; Preferred glucose 10 grams per liter, whiteruss 15 grams per liter, yeast extract paste 3.0 grams per liter, magnesium sulfate 0.2 grams per liter, Repone K 0.2 grams per liter, potassium primary phosphate 2.0 grams per liter, urea 1.0 grams per liter, ammonium nitrate 1.5 grams per liter, tween-80 0.5 milliliter/liter, Trace salts solution 1.0 milliliters/liter.
Wherein: often liter of Trace salts solution contains 11.0 grams, zinc sulfate, manganous sulfate 6.0 grams, 1.0 grams, ferrous sulfate, EDTA-Na
27.3 grams, rose vitriol 0.3 gram, 40 milligrams, copper sulfate, boric acid 60 milligrams, potassiumiodide 1 milligram.
Candida lipolytica Y-57(Candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
Pseudomonas putida P-101(Pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
The application of pseudomonas putida P-101 in greasy filth separating treatment of described deposit number to be the Candida lipolytica Y-57 of CGMCC No.5790 and/or described deposit number be CGMCC No.5801.
Beneficial effect:
The present invention adopts microbial treatment method, is separated by oil in greasy filth, reaches the effect of oily resource recovery, and do not add the chemical agent to environmental effects, do not produce secondary pollution with mud.Carrying out recycling treatment to sludge containing, can obtain certain economic benefit, is a kind of cost-effective oil-sludge treatment technology.
The present invention is directed to concrete greasy filth to detect, the micro-nutrients added is determined according to the nitrogen element of greasy filth self and phosphoric composition and content, promote microbial growth and the metabolism of the ability of the degraded heavy oil with uniqueness and the generation tensio-active agent added, have more specific aim.
The two high-efficiency degradation petroleum-type microorganisms obtained are screened in trench greasy filth near the Candida lipolytica Y-57 added in the present invention and pseudomonas putida P-101 Shi Cong restaurant, this two strains bacterium is applied to greasy filth to be separated, improve the separating effect of oil and mud in greasy filth, and the degradation effect of irreducible oil in mud and (or) water, oil-contg < 2%(residual in mud is in dry mud).
Biomaterial preservation information
P-101, Classification And Nomenclature is pseudomonas putida (Pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
Y-57, Classification And Nomenclature is Candida lipolytica (Candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
Embodiment
Embodiment 1
Choose trench greasy filth near Yixing City restaurant to screen by the following method:
1.1 bacterial classification enrichments
Add certain greasy filth (2%) in dextrose culture-medium in triangular flask and put into shaking table, cultivate 3 days, obtain bacterial classification pregnant solution for 30 DEG C.Dextrose culture-medium formula is: sodium-chlor 1g, calcium chloride 0.5g, dipotassium hydrogen phosphate 0.5g, potassium primary phosphate 0.5g, iron(ic) chloride 0.02g, ammonium nitrate 1g, magnesium sulfate heptahydrate, 1g yeast extract, 2g glucose, 1L water, PH 7.0-7.2; 110 DEG C of sterilizing 20min.
1.2 bacterial screening
By after the dilution of bacterial classification pregnant solution again dextrose solid medium flat board (adding 2% agar in dextrose culture-medium)) on to rule separation, cultivate 24 hours in 30 DEG C, picking in the good single bacterium colony streak inoculation of grow on plates on alkane flat board, cultivate 48 hours in 30 DEG C, choose maximum two single bacterium colonies called after Y-57, P-101 respectively.
Embodiment 2
Carry out biology and biochemical test to Y-57 bacterial strain, this yeast YPD Agar is incubated at flat board (30 DEG C) 3 days, and bacterium colony can grow to 1-2 millimeter, and white is to cream-colored.Initial stage bacterium colony is smooth shape, occurs gauffer through Extending culture bacterium colony.When solid or liquid nutrient medium grow, the rounded or oval of cellular form, cell also can elongate the false filamentous growth in class sometimes, and cell size is about 4-6 × 6-8 micron.
This yeast Biolog microbe auto-analysis instrument that Biolog company of the U.S. produces is identified, is accredited as Candida lipolytica.
Carry out biology and biochemical test to P-101 bacterial strain, the aerobic cultivation of this bacterium nutrient agar plate 24 hours, bacterial growth is vigorous, and bacterium colony is smooth, moistening, translucent faint yellow bacterium colony, produces strong smell, gramstaining is negative bacillus, and extremely raw many flagellums, most bacterial strain flagellum is more than three.This bacterium obligate aerobic, breathing pattern metabolism, decomposition glucose and wood sugar, utilize citritase, and Phenylalanine dehydrogenase reaction is feminine gender.Utilize inositol, oxydase and Terminal oxidase positive, arginine dihydrolase is positive, does not produce Polylevulosan, not gelatin hydrolysate, not hydrolyzed starch, hydrolysis polychrom, egg yellow reaction is negative, and Poly-β-hydroxybutyric Acid salt is negative, reduction nitrate, U.S. red negative, voges-Proskauer test is negative, and indole test is negative; 4 DEG C can grow, and 41 DEG C do not grow; Appropriate pH is 7-8.5.
This bacterium Biolog microbe auto-analysis instrument that Biolog company of the U.S. produces is identified, is accredited as pseudomonas putida.
Candida lipolytica Y-57 of the present invention and pseudomonas putida P-101 on February 24th, 2012 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, wherein, Candida lipolytica Y-57 deposit number is CGMCCNO.5790, and Classification And Nomenclature is: Candida lipolytica (Candida lipolytica); Pseudomonas putida P-101 deposit number is CGMCC NO.5801, and Classification And Nomenclature is: pseudomonas putida (Pseudomonas putida).
Embodiment 3
(1) the greasy filth sample of North China Oilfield slurry tank is got, to process greasy filth sampling analysis: measuring oleaginousness according to " agricultural sludge method for monitoring and analyzing " is 10%, adopt ion method heavy metal, every heavy metal index is all less than pollutant catabolic gene standard (GB4284-84) in agricultural sludge, adopting alkaline chitinase to measure total nitrogen in greasy filth is 35mg/L, with in Ammonium Molybdate Spectrophotometric Method for Determination greasy filth total phosphorus 12mg/L.
(2) Candida lipolytica Y-57(CGMCC NO.5790) and pseudomonas putida P-10(CGMCC NO.5801) use LB substratum respectively, at 30 DEG C, 200rpm shaking table carries out cultivation 24 hours, fermention medium is seeded to again according to the inoculum size of 5%, in 30 DEG C, 200rpm stirs, air quantity 1:0.4-0.5, cultivates 36 hours under tank pressure 0.05mpa.
Fermention medium (grams per liter): glucose 10, whiteruss 15, yeast extract paste 3.0, magnesium sulfate 0.2, Repone K 0.2, potassium primary phosphate 2.0, urea 1.0, ammonium nitrate 1.5, tween-80 0.5ml, Trace salts solution 1.0ml.PH nature.
Wherein: often liter of Trace salts solution contains 11.0 grams, zinc sulfate, manganous sulfate 6.0 grams, 1.0 grams, ferrous sulfate, EDTA-Na
27.3 grams, rose vitriol 0.3 gram, 40 milligrams, copper sulfate, boric acid 60 milligrams, potassiumiodide 1 milligram.
(3) in pending greasy filth, add 0.1wt%(by greasy filth weighing scale) described in the mixed culture of deposit number to be the Candida lipolytica Y-57 of CGMCC No.5790 and deposit number the be pseudomonas putida P-101 of CGMCC No.5801; Determine to add potassium primary phosphate 60mg/L, urea 360mg/L and tween-80 500mg/L according to nitrogen element and phosphoric composition and content in process greasy filth; Stir under PH=7.5, temperature 22 DEG C, 100rpm, ventilate, cultivate more than 8 hours, oil is separated with mud, and oil can float on upper strata, and measuring oil-contg residual in mud according to " agricultural sludge method for monitoring and analyzing " is that 1.7%(is in dry mud).
Claims (7)
1. improve a method for greasy filth separation treatment efficiency, it is characterized in that comprising following steps:
(1) to process greasy filth sampling analysis;
(2) micro-nutrients that adds and/or tensio-active agent is determined according to nitrogen element and phosphoric composition and content in process greasy filth;
(3) in pending greasy filth, by the dosage of 0.01%-1wt%, to add deposit number be the Candida lipolytica Y-57 of CGMCC No.5790 and deposit number is the mixed culture of the pseudomonas putida P-101 of CGMCC No.5801, and add micro-nutrients and/or tensio-active agent, at pH=6-10, temperature 8-45 DEG C, 50-150rpm stirs, ventilates, cultivate more than 8 hours, the oil in greasy filth is separated with mud.
2. the method for raising greasy filth separation treatment efficiency according to claim 1, when it is characterized in that in described process greasy filth, ammonia nitrogen concentration is less than 200mg/L, adds nitrogenous source micro-nutrients and is at least 200mg/L to total nitrogen concentration; When total phosphorus concentration is less than 25mg/L in oil-sludge treatment system, adds phosphorus source micro-nutrients and be at least 25mg/L to total phosphorus concentration.
3. the method for raising greasy filth separation treatment efficiency according to claim 2, it is characterized in that described nitrogenous source micro-nutrients is ammonium chloride or urea, described phosphorus source micro-nutrients is phosphoric acid salt, and described tensio-active agent is selected from one or more of tween, sapn, rhamnolipid, alkylaryl polyglycol ether, alkyl polyglycol ether or alkylaryl sulphonate.
4. the method for raising greasy filth separation treatment efficiency according to claim 1, is characterized in that the quantity of effective microbe in the mixed culture of described Candida lipolytica Y-57 and pseudomonas putida P-101 is greater than 10
5cfu/mL.
5. Candida lipolytica Y-57(
candida lipolytica), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5790.
6. pseudomonas putida P-101(
pseudomonas putida), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation day is on February 24th, 2012, and deposit number is CGMCC No.5801.
7. Candida lipolytica Y-57 according to claim 5 and the application of pseudomonas putida P-101 according to claim 6 in greasy filth separating treatment.
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