CN103444511B - A kind of breeding method of rice indica hybrid parent round-grained rice restorer - Google Patents
A kind of breeding method of rice indica hybrid parent round-grained rice restorer Download PDFInfo
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- CN103444511B CN103444511B CN201310353953.1A CN201310353953A CN103444511B CN 103444511 B CN103444511 B CN 103444511B CN 201310353953 A CN201310353953 A CN 201310353953A CN 103444511 B CN103444511 B CN 103444511B
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Abstract
The invention provides the breeding method of a kind of rice indica hybrid parent round-grained rice restorer, comprise the following steps: maternal structure: select high-combining ability restorer and Japonica restorer line (preferably carrying wide compatibility) to hybridize, obtain maternal; Male parent builds: select high-combining ability restorer and two-line hybrid rice to commonly use restorer and hybridize, obtain male parent; Crossbreeding: female parent and paternal hybrid are cultivated to obtain rice indica hybrid parent round-grained rice restorer.This breeding method utilizes indica-japonica hybrid intermediate materials as bridge, and adopt the mode that Xian round-grained rice Xian reestablishes diplomatic relations, overcome the reproductive disorder between Xian round-grained rice, progeny material is stablized, and reaches the object of fixing Xian round-grained rice advantage fast; Extensive 82 grouting in rice indica hybrid parent round-grained rice restorer town that the method obtains are quick, full seed, and high yield resists more, and have wide spectrum restoring force, general combining ability is high.
Description
Technical field
The invention belongs to field of crop genetic breeding, particularly a kind of breeding method of rice indica hybrid parent round-grained rice restorer.
Background technology
Output is high owing to having for hybrid rice, wide adaptability, easy advantage of cultivating, and cultivated area accounts for 55% of the national paddy rice gross area, and output accounts for more than 60% of paddy rice gross yield, for China's grain-production makes a great contribution.Although China's hybrid rice breeding achieves huge progress, but be also faced with the situation that cultivated area and unit yield fluctuate for many years, along with people subtracts with increasing, food problem remains the social concern that can not be ignored, therefore, cultivate that in rice matter, output, have breakthrough New Hybrid Rice Combination be become China's breeding of hybridized rice problem in the urgent need to address.
The seed selection of hybrid rice restoring line is the important step of rice heterosis utilization, and along with the performance of intervarietal hybrid use of advantage potentiality, breeding man starts that sight is invested the larger inter-subspecific heterosis of potentiality and utilizes.Now, adopt the modes such as indica-japonica hybrid, Xian round-grained rice Xian reestablish diplomatic relations, infiltrate a certain proportion of japonica rice blood relationship, the indica type close Japonica restorer line of seed selection in Indica Rice Restorer Lines, part utilizes inter-subspecific heterosis, has become the important research direction of Breeding for restoration lines.But owing to there is larger difference between indica rice genome, cause indica-japonica hybrid to there is reproductive disorder, offspring is difficult to stablize.
Summary of the invention
Goal of the invention: the breeding method that the object of this invention is to provide a kind of rice indica hybrid parent round-grained rice restorer.
Technical scheme: the breeding method of a kind of rice indica hybrid parent round-grained rice restorer provided by the invention, is characterized in that: comprise the following steps:
(1) maternal structure: select high-combining ability restorer and Japonica restorer line to hybridize, obtain maternal;
(2) male parent builds: select high-combining ability restorer and two-line hybrid rice to commonly use restorer and hybridize, obtain male parent;
(3) crossbreeding: female parent and paternal hybrid are cultivated to obtain rice indica hybrid parent round-grained rice restorer.
Preferably, specifically comprise the following steps:
(1) maternal structure: select high-combining ability restorer Zhenhui 084 and Japonica restorer line 351 to hybridize, obtain maternal (084/351) F
4;
(2) male parent builds: select high-combining ability restorer Zhenhui 084 and two-line hybrid rice to commonly use restorer 9311 and hybridize, obtain male parent (084/9311) F
6;
(3) crossbreeding: with (084/351) F
4for female parent, with (084/9311) F
6for male parent, rice indica hybrid parent round-grained rice restorer town extensive 82 is cultivated to obtain in hybridization.
Wherein, in step (1), maternal structure need through the selection in continuous 4 generations.
Wherein, in step (2), male parent builds need through the selection in continuous 6 generations.
Present invention also offers a kind of utilization above-mentionedly educates the hybridization of rice indica hybrid parent round-grained rice restorer obtains the breeding method of hybrid rice, comprises the following steps: recover to be male parent with rice indica hybrid parent round-grained rice, with sterile be maternal, be mixed with hybrid rice.
Wherein, described rice indica hybrid parent round-grained rice recovers to be rice indica hybrid parent round-grained rice restorer town extensive 82.
Wherein, described sterile be the temperature sensitive two-line sterile line of CMS line or light.
Wherein, described sterile be CMS line town Xian 1A, the temperature sensitive two-line sterile line of described light is the temperature sensitive two-line sterile line 1892S of light.
Beneficial effect: breeding method provided by the invention utilizes indica-japonica hybrid intermediate materials as bridge, adopt the mode that Xian round-grained rice Xian reestablishes diplomatic relations, overcome the reproductive disorder between Xian round-grained rice, progeny material is at F
5basicly stable, reach the object of fixing Xian round-grained rice advantage fast; Extensive 82 grouting in rice indica hybrid parent round-grained rice restorer town that the method obtains are quick, full seed, and high yield resists more, and have wide spectrum restoring force, general combining ability is high.
Specifically, the rice indica hybrid parent round-grained rice restorer town extensive 82 of acquisition has following outstanding advantage relative to prior art:
1, have wide spectrum restoring force, general combining ability is high
Town extensive 82 shaping before select representative two-line sterile line and CMS line and the continuous test cross of candidate's strain respectively, identify hybrid vigour, preferentially choose the high strain of coordinate force and obtain restorer of the present invention.The restorer town extensive 82 obtained can to CMS line have restoring force again can with two-line sterile line combo, have wide spectrum restoring force, general combining ability is high.
2, extensive 82 grouting in restorer town of the present invention fast, full seed
Because town extensive 82 has organically combined Xian round-grained rice advantage, within 28 days, be full maturity at Post flowering, 7-10 days faster than conventional restorer, and full seed, thousand kernel weight reaches 30.25 grams.
3, high yield resists more
Extensive 82 continuous 2 years (2011-2012) in restorer town of the present invention participate in our company's variety comparative test, amount to per mu yield average out to 625.50 kilograms, suitable with the yield level of hybrid rice.Extensive 82 high resistance to hoja blanca in town, the resistance of rice blast is reached in anti-.
Accompanying drawing explanation
Fig. 1 is the breeding pedigree schematic diagram of the breeding of rice indica hybrid of the present invention parent round-grained rice restorer.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The breeding of rice indica hybrid parent round-grained rice restorer, specific as follows:
1, the structure of parent
During 2002-2005, utilize high-combining ability restorer Zhenhui 084 (084) and Japonica restorer line 351 to hybridize, through the selection in continuous 4 generations, obtain intermediate materials 9902, i.e. (084/351) F
4; Utilize 084 to commonly use restorer 9311 with two-line hybrid rice to hybridize, the selection through continuous 6 generations is stablized, and obtains intermediate materials 9987, i.e. (084/9311) F simultaneously
6.
2, crossbreeding:
2005, in Hainan, with indica-japonica hybrid intermediate materials (084/351) F
4for female parent, with excellent intermediate materials (084/9311) F
6for male parent, indica type close round-grained rice restorer town extensive 82 is cultivated in hybridization.
F
2start to select individual plant from generation to generation; Comfortable [(084/351) F
4// (084/9311) F
6] F
2rise, according to seed holding, grain filling rate in conjunction with the defect individual of the indica type close round-grained rice of comprehensive agronomy proterties M8003 line in colony.
F
3play strain roguing from generation to generation to stablize, simultaneously with reference to Jiangsu Province's variety certification standard inoculation bacterial leaf spot, rice blast pathogen, make Resistance Identification.
F
5rise from generation to generation and select elite plant strain and CMS line Zhenshan 97a, two-line sterile line Guangzhan 63S test cross, within continuous 2 years, investigate hybridization F
1hybrid vigour (containing proterties such as output, breeding time, ripening rate, quality, resistances), select optimum strain.
To F
8obtain stable restorer town extensive 82, its biological property is as follows:
1, Characters of Plant Type: extensive 82 tillering abilities in town are medium, panicle number per plant is at about 12, and plant height about 115cm, Leaves on main stem 18-19 opens, and sword-like leave is short very, and plant type is collecting and distributing moderate.
2, yield traits: extensive 82 Spike-grain structure in town are reasonable, and number of grain per ear 150.5, ripening rate is up to 95%, and thousand kernel weight high (30.25 grams), average yield per mu reaches 625.50 kilograms, suitable with hybrid rice.
3, prominent features: the extensive 82 later stage filling speeds in town are fast, spending latter 28 days is full maturity, 7-10 days more Zao than general restorer, and full seed.Basal internode is sturdy, and lodging resistance is given prominence to.
The present invention is not limited to the parent of above embodiment when implementing, and can be existing any kind, all by following methods breeding:
(1) maternal structure: select high-combining ability restorer and Wide compability Japonica restorer line to hybridize, obtain maternal;
(2) male parent builds: select high-combining ability restorer and two-line hybrid rice to commonly use restorer and hybridize, obtain male parent;
(3) crossbreeding: female parent and paternal hybrid are cultivated to obtain rice indica hybrid parent round-grained rice restorer.
Wherein, high-combining ability restorer can select town extensive 42, in extensive 9308 etc., Japonica restorer line can select C418, Lemont etc., and two-line hybrid rice is commonly used restorer and raised rice No. 6, another name for Sichuan Province extensive 527.
Embodiment 2
The breeding of hybrid rice, specific as follows:
With high-quality CMS line town Xian 1A for female parent, with town extensive 82 for male parent, preparation combination Xian 1A/ town, town extensive 82(town Xian excellent 82), the variety comparative test average yield per mu of continuous 2 years reaches 680.5 kilograms.
With the temperature sensitive two-line sterile line 1892S of light for female parent, with town extensive 82 for male parent, the preparing hybrid combination extensive 82(9 in 1892S/ town excellent 82), within continuous 2 years, participate in our company's variety comparative test, per mu yield reaches 705.1 kilograms.
As can be seen here, extensive 82 general combining abilities in town are higher, restoring force wide spectrum, is identified by the Hybrid Rice Combination advantage prepared with dissimilar two-line sterile line and CMS line and finds that namely this restorer can be used as the male parent of CMS line, can obtain influential point set again with two-line sterile line combo.
Wherein, town Xian 1A, 1892S are commercially available.
Claims (2)
1. a breeding method for rice indica hybrid parent round-grained rice restorer, is characterized in that: specifically comprise the following steps:
(1) maternal structure: select high-combining ability restorer Zhenhui 084 and Japonica restorer line 351 to hybridize, obtain maternal (084/351) F
4;
(2) male parent builds: select high-combining ability restorer Zhenhui 084 and two-line hybrid rice to commonly use restorer 9311 and hybridize, obtain male parent (084/9311) F
6;
(3) crossbreeding:
With indica-japonica hybrid intermediate materials (084/351) F
4for female parent, with excellent intermediate materials (084/9311) F
6for male parent, indica type close round-grained rice restorer town extensive 82 is cultivated in hybridization;
F
2start to select individual plant from generation to generation; Comfortable [(084/351) F
4// (084/9311) F
6] F
2rise, according to seed holding, grain filling rate in conjunction with the defect individual of the indica type close round-grained rice of comprehensive agronomy proterties M8003 line in colony;
F
3play strain roguing from generation to generation to stablize, simultaneously with reference to Jiangsu Province's variety certification standard inoculation bacterial leaf spot, rice blast pathogen, make Resistance Identification;
F
5rise from generation to generation and select elite plant strain and CMS line Zhenshan 97a, two-line sterile line Guangzhan 63S test cross, within continuous 2 years, investigate hybridization F
1hybrid vigour, comprise output, breeding time, ripening rate, quality and resistance trait, select optimum strain;
To F
8obtain stable restorer town extensive 82.
2. a breeding method for hybrid rice, is characterized in that: comprise the following steps: recover to be male parent with rice indica hybrid parent round-grained rice, with sterile be maternal, be mixed with hybrid rice; Described rice indica hybrid parent round-grained rice recovers to be the town extensive 82 that method described in claim 1 obtains; Described sterile be the temperature sensitive two-line sterile line of CMS line or light, described CMS line is town Xian 1A, and the temperature sensitive two-line sterile line of described light is 1892S.
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| CN105309300B (en) * | 2014-07-29 | 2017-10-24 | 安徽省农业科学院水稻研究所 | A kind of method that seed selection is difficult the High-Yielding Hybrid Rice of shattering |
| CN106342679A (en) * | 2016-08-24 | 2017-01-25 | 云南大学 | Breeding method of perennial rice and application |
| CN106561443A (en) * | 2016-09-29 | 2017-04-19 | 湖北大学 | Polyploidy rice two-line restoring line breeding technology |
| CN113243293B (en) * | 2021-06-23 | 2022-12-09 | 武汉亘谷源生态农业科技有限公司 | Breeding method of super hybrid rice between subspecies of indica rice and japonica rice |
| CN113661918B (en) * | 2021-06-29 | 2022-08-19 | 高邮周邶墩农业发展有限公司 | Breeding method of intermediate wide-affinity restoring line with high alkali value elimination and indica-japonica |
| CN115644049A (en) * | 2022-10-13 | 2023-01-31 | 湖南省棉花科学研究所 | Method for improving rice indica-japonica hybrid seed setting rate |
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| JPH0322927A (en) * | 1989-06-19 | 1991-01-31 | Mitsui Toatsu Chem Inc | Rice plant having novel cytoplasm and its cultivation |
| CN1028471C (en) * | 1990-07-17 | 1995-05-24 | 邓祚荣 | Two system method crossbreeding technology for long-grained nonglutinous rice subspecised |
| CN1559189A (en) * | 2004-02-27 | 2005-01-05 | 华南农业大学 | Breeding method for rice of japonica type affined to indica rice restoring series |
| CN1817096A (en) * | 2005-12-27 | 2006-08-16 | 辽宁省稻作研究所 | Japonica rice restoring system breeding method by indica and japonica bridge technology |
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