CN103431202B - Anti-stress reinforcing agent for portunus trituberculatus and application thereof - Google Patents
Anti-stress reinforcing agent for portunus trituberculatus and application thereof Download PDFInfo
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- CN103431202B CN103431202B CN201310299644.0A CN201310299644A CN103431202B CN 103431202 B CN103431202 B CN 103431202B CN 201310299644 A CN201310299644 A CN 201310299644A CN 103431202 B CN103431202 B CN 103431202B
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Abstract
The invention discloses an anti-stress reinforcing agent for portunus trituberculatus and an application thereof, and is characterized in that the anti-stress reinforcing agent is prepared by evenly mixing the following raw materials in parts by weight: 3-5 parts of glutamine dipeptide, 15-20 parts of vitamin C-2-single-polyphosphate, 10-15 parts of vitamin E acetate, 10-20 parts of taurine, 10-15 parts of citric acid, 1-3 parts of soybean flavonoids, and 30-50 parts of glucose. The mass percentage content of the anti-stress reinforcing agent in a feeding feed is 0.06-0.08%. The anti-stress reinforcing agent has the advantages that: the adding amount of the anti-stress reinforcing agent in the feed is only 0.06-0.08%, so that the resistance of portunus trituberculatus on pathogenic microorganisms and the ability of portunus trituberculatus to resist the environmental stress can be significantly improved, thus the survival rate of portunus trituberculatus in full cycle aquaculture is improved, the survival rate can be increased by 16.5-19%, the average body weight can be increased by more than 10%, and the aquaculture yield is increased by 26.9-31.3%.
Description
Technical field
The present invention relates to the feed addictive of Portunus trituberculatus Miers, especially relating to that a kind of Portunus trituberculatus Miers is anti-stress reinforcing agent and application thereof.
Background technology
Under the intensive cultivating condition of crab class, the crab class of cultivation is subject to environmental factor as temperature, salinity and pH etc., chemical factor is as Heavy Metals in Waters, ammonia nitrogen etc., the physiological stress reaction that biological factor causes as acute variation such as microorganisms, affect breeding, growth, the disease-resistant and survival ability of crab class, thereby cause cultivated crabs class survival rate lower, the speed of growth is slower, even cause degradation under the quality of crab meat.Therefore need exploitation crab class anti-stress feed addictive, to improve the survival rate of crab class, promote the growth of crab class.Chinese Patent Application No. is CN201010595413.0, name is called the anti-of large yellow croaker stress reinforcing agent, disclose by vitamin A, the mono-polyphosphate of vitamin C-2-, Vitwas E, Astragalus Root P.E, sodium selenite, copper methionine, zinc methionine and glucose are that the even mixed preparing of raw material forms; This is anti-stress reinforcing agent addition in feed be only 0.1～0.2%, just significantly improves the ability of large yellow croaker to the resistance of pathogenic microorganism and anti-environment-stress.But, also not disclose at present both at home and abroad any about Portunus trituberculatus Miers resist stress reinforcing agent relevant report and application.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of survival rate can improve 16.5-19%, and body weight can improve that the Portunus trituberculatus Miers of 26.9-31.3% is anti-stress reinforcing agent and application thereof.
The present invention solves the problems of the technologies described above adopted technical scheme: the anti-of a kind of Portunus trituberculatus Miers stress reinforcing agent; the even mixed preparing of raw material by following portions by weight forms: glutamine dipeptide 3-5; the mono-polyphosphate 15-20 of vitamin C-2-; Vitwas E 10-15; taurine 10-20; citric acid 10-15; soybean flavonoids 1-3; glucose 30～50; soybean flavonoids by soybean by orientation extract, concentrate drying forms; active ingredient is flavones, and valid density is 10-15%.
The anti-of Portunus trituberculatus Miers stress a reinforcing agent, is formed: glutamine dipeptide 4, the mono-polyphosphate 18 of vitamin C-2-by the even mixed preparing of raw material of following portions by weight; Vitwas E 12, taurine 15, citric acid 12; soybean flavonoids 2, glucose 39.
An anti-application that stress reinforcing agent that Portunus trituberculatus Miers becomes that crab uses, this is anti-stress the mass percentage content of reinforcing agent in the feed of throwing something and feeding be 0.06-0.08%.
Selected raw material in the present invention: glutamine dipeptide has the Portunus trituberculatus Miers non-specific immunity of raising and anti-stress ability; Vitamin C and vitamin E are biological anti-oxidant, remove the peroxylradicals in Portunus trituberculatus Miers body, and have the crab of raising class anti-stress effect; Soybean flavonoids has removes free radical and antioxidation, to Portunus trituberculatus Miers and have and protect the liver, protect liver function effect; Taurine promotes the lymphocytic growth and breeding of Portunus trituberculatus Miers immune system, and has heat-clearing, analgesic activity, and then strengthens the resistance of Portunus trituberculatus Miers; Citric acid becomes soluble complexes with calcium binding, can alleviate the effect that calcium ion impels blood clotting, to Portunus trituberculatus Miers, can play blood coagulation resisting function; Glucose can slow down because the physiology that stress cause is uncomfortable, improves Portunus trituberculatus Miers anti-stress ability.This compound anti-stress reinforcing agent for the Portunus trituberculatus Miers shell phase factors such as vulnerable and environment and stress waiting of causing has remarkable promotion Growth of Portunus Trituberculatus, improve Portunus trituberculatus Miers anti-stress ability.
Compared with prior art, the invention has the advantages that: the anti-of a kind of Portunus trituberculatus Miers of the present invention stress reinforcing agent, by glutamine dipeptide, the mono-polyphosphate of vitamin C-2-, Vitwas E, taurine, citric acid, soybean flavonoids, glucose is that the even mixed preparing of raw material forms; This is anti-stress reinforcing agent addition in feed be only 0.06～0.08%, just significantly improve the ability of Portunus trituberculatus Miers to the resistance of pathogenic microorganism and anti-environment-stress, thereby improve the survival rate of Portunus trituberculatus Miers complete period cultivation, survival rate can improve 16.5-19%, average weight can improve 26.9-31.3%, and cultured output improves 16.5-19%.Therefore the present invention is that a kind of survival rate can improve 16.5-19%, and average weight can improve 26.9-31.3%, and the anti-of the good Portunus trituberculatus Miers of crab meat quality stress reinforcing agent.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The anti-of a kind of Portunus trituberculatus Miers stress reinforcing agent; by following parts by weight, take: glutamine dipeptide 4; the mono-polyphosphate 18 of vitamin C-2-; Vitwas E 12, taurine 15, citric acid 12; soybean flavonoids 2; glucose 39, evenly mixes above-mentioned each raw material, be prepared into anti-stress reinforcing agent.
Embodiment 2
The anti-of Portunus trituberculatus Miers stress reinforcing agent; substantially the same manner as Example 1; difference is in composition of raw materials: glutamine dipeptide is 3, and the mono-polyphosphate of vitamin C-2-is 20, and Vitwas E is 10; taurine is 20; citric acid 10, soybean flavonoids 1, glucose 30; above-mentioned each raw material is evenly mixed, be prepared into anti-stress reinforcing agent.
Embodiment 3
The anti-of Portunus trituberculatus Miers stress reinforcing agent; substantially the same manner as Example 1; difference is in composition of raw materials: glutamine dipeptide is 5, and the mono-polyphosphate of vitamin C-2-is 15, and Vitwas E is 15; taurine is 10; citric acid is 15, and soybean flavonoids is 3, and glucose is 50; above-mentioned each raw material is evenly mixed, be prepared into anti-stress reinforcing agent.
Test example
Breed variety: 2.5 grams of Portunus trituberculatus Miers juvenile crab 4000 tails, Random assignment is 4 groups, every group of 5 repetitions, each cement pit (specification is 3m * 1.5m * 1m, long * wide * dark) cultivation Portunus trituberculatus Miers 200 tails, it is identical that initial body weight is respectively organized in experiment; Cultivating condition is as basic identical in environmental factor, chemical factor and biological factor.
Aquaculture management: other condition is basic identical, be added with 0.06%(weight in the different just cement pit feed of throwing something and feeding for 1 every day) the anti-of the Portunus trituberculatus Miers of embodiment 1 stress reinforcing agent; In the feed that cement pit is thrown something and fed for 2 every days, being added with 0.06%(weight) the anti-of the Portunus trituberculatus Miers of embodiment 2 stress reinforcing agent; In the feed that cement pit is thrown something and fed for 3 every days, being added with 0.06%(weight) the anti-of the Portunus trituberculatus Miers of embodiment 3 stress reinforcing agent; In the feed that cement pit is thrown something and fed for 4 every days, not adding the anti-of Portunus trituberculatus Miers stress reinforcing agent.
Result comparison: cultivate after 8 weeks, calculate the Portunus trituberculatus Miers mantissa of each cement pit, be calculated to be motility rate; The all crabs of each cement pit are weighed simultaneously, calculate rate of body weight gain.Experimental result is as shown in table 1.As can be seen from Table 1, cement pit 1,2,3 survival rates improve 16.5-19% than not interpolation group, add and resist and stress reinforcing agent group average weight improve 26.9-31.3% than not interpolation group.Illustrate that the anti-of Portunus trituberculatus Miers of the present invention stress have advantages of survival rate and the quickening Growth of Portunus Trituberculatus speed that improves Portunus trituberculatus Miers by reinforcing agent.
The Portunus trituberculatus Miers survival rate of each cement pit of table 1 and average weight table
| ? | Cement pit 1 | Cement pit 2 | Cement pit 3 | Cement pit 4 |
| Total mantissa | 164 | 161 | 166 | 128 |
| Survival rate % | 82.0 | 80.5 | 83.0 | 64.0 |
| Average weight | 27.3 | 26.9 | 27.8 | 21.2 |
Above-described embodiment is to further illustrate of the present invention, but protection scope of the present invention is as the criterion with claims.
Claims (3)
1. the anti-of a Portunus trituberculatus Miers stress reinforcing agent; it is characterized in that being formed by the even mixed preparing of raw material of following portions by weight: glutamine dipeptide 3-5; the mono-polyphosphate 15-20 of vitamin C-2-, Vitwas E 10-15, taurine 10-20; citric acid 10-15; soybean flavonoids 1-3, glucose 30～50, soybean flavonoids by soybean by orientation extract, concentrate drying forms; active ingredient is flavones, and valid density is 10-15%.
2. the anti-of a kind of Portunus trituberculatus Miers according to claim 1 stress reinforcing agent; it is characterized in that being formed by the even mixed preparing of raw material of following portions by weight: glutamine dipeptide 4; the mono-polyphosphate 18 of vitamin C-2-; Vitwas E 12; taurine 15; citric acid 12, soybean flavonoids 2, glucose 39.
3. an anti-application that stress reinforcing agent for Portunus trituberculatus Miers according to claim 1, is characterized in that: claimed in claim 1 anti-stress the mass percentage content of reinforcing agent in the feed of throwing something and feeding be 0.06-0.08%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310299644.0A CN103431202B (en) | 2013-07-17 | 2013-07-17 | Anti-stress reinforcing agent for portunus trituberculatus and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310299644.0A CN103431202B (en) | 2013-07-17 | 2013-07-17 | Anti-stress reinforcing agent for portunus trituberculatus and application thereof |
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| CN103431202A CN103431202A (en) | 2013-12-11 |
| CN103431202B true CN103431202B (en) | 2014-09-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103844139B (en) * | 2013-12-23 | 2015-08-26 | 浙江省海洋开发研究院 | A kind of mixed feed for cultivating soft shell Portunus trituberculatus Miers and preparation method thereof |
| CN105519816B (en) * | 2016-01-29 | 2019-08-16 | 济南和美华饲料有限公司 | A kind of additive improving laying hen anti-stress ability |
| CN105851523A (en) * | 2016-04-06 | 2016-08-17 | 宁波大学 | Stress resistance reinforcing agent for harvestfishes and application of stress resistance reinforcing agent |
| CN106616037B (en) * | 2016-12-20 | 2022-02-08 | 集美大学 | Taurine composite preparation for grouper and application thereof |
| CN108029901A (en) * | 2018-01-04 | 2018-05-15 | 广东海洋大学 | A kind of feed addictive of raising Duo Lin Xi intestinal beneficial bacterium quantity |
| CN108185220A (en) * | 2018-01-04 | 2018-06-22 | 广东海洋大学 | A kind of feed addictive for promoting red coat intestinal health |
| CN108094761A (en) * | 2018-01-04 | 2018-06-01 | 广东海洋大学 | A kind of feed addictive for promoting the field planting of egg-shaped pompano intestinal beneficial microbes |
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| CN100421575C (en) * | 2005-09-30 | 2008-10-01 | 苏州大学 | Anti-stress health-care agent for shrimp and crab |
| CN101357223B (en) * | 2008-08-30 | 2013-02-13 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of anti-stress agent for fishing |
| CN101720869A (en) * | 2009-12-07 | 2010-06-09 | 中国水产科学研究院淡水渔业研究中心 | Method for preparing immunostimulant for fish |
| CN102038109B (en) * | 2010-12-20 | 2012-06-13 | 宁波大学 | Antistress reinforcing agent for large yellow croaker |
| CN102133392B (en) * | 2011-03-01 | 2012-09-05 | 中国水产科学研究院淡水渔业研究中心 | Water splashing compound preparation for boosting exuviation of shrimps and crabs, hardening shells, preventing diseases and resisting stresses |
| CN102178080B (en) * | 2011-04-12 | 2012-11-21 | 吉林农业大学 | Feeding method capable of promoting growth and increasing immunity and stress resistance of Cyprinus carpio var. jian |
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