CN103421868A - Preparation method of tuna skin collagen micro-molecular peptides - Google Patents
Preparation method of tuna skin collagen micro-molecular peptides Download PDFInfo
- Publication number
- CN103421868A CN103421868A CN2012101730089A CN201210173008A CN103421868A CN 103421868 A CN103421868 A CN 103421868A CN 2012101730089 A CN2012101730089 A CN 2012101730089A CN 201210173008 A CN201210173008 A CN 201210173008A CN 103421868 A CN103421868 A CN 103421868A
- Authority
- CN
- China
- Prior art keywords
- tuna
- enzymatic hydrolysis
- preparation
- raw material
- molecular peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of tuna skin collagen micro-molecular peptides. The preparation method comprises the following steps: immersing and cleaning tuna skins, crushing the tuna skins by using a crusher, adding to an industrial grade colloid mill, carrying out ultramicro crushing to a particle size of 30-50mum, uniformly mixing with water having a volume 2-6 times the volume of obtained particles, adding to a centrifuge, continuously centrifuging at 8000-12000rpm for 30min, removing the obtained supernatant, adding the obtained precipitate and water having a volume 3-5 times the volume of the obtained precipitate to a biological enzymatic hydrolysis pot, carrying out enzymatic hydrolysis under conditions comprising a temperature of 37-50DEG C and a pH value of 7.5-8.0 for 2-4h, processing the obtained enzymatic hydrolysis product by a 3KD ultrafilter membrane, carrying out nanofiltration membrane concentration of the part having a molecular weight below 3KD in the product, and carrying out spray drying to obtain the finally-finished collagen micro-molecular peptides polypeptides, wherein the addition amount of a composite enzyme in the above enzymatic hydrolysis reaction is 0.5-2% of the amount of a raw material. The part having a molecular weight above 3KD in the enzymatic hydrolysis product returns to the biological enzymatic hydrolysis pot for enzymatic hydrolysis.
Description
Technical field
The invention belongs to a kind of method that sea-food extracts, specifically a kind of extraction preparation method of collagen of fish skin.
Technical background
At present, the tuna skin is as the tankage of the tuna course of processing, as waste or production low value feed, economic worth is lower, and tuna is as a kind of high-quality marine economy fish, and market demand is huge, the tuna skin quantity produced is large, wherein is rich in the high-quality active collagen.Collagen protein, as a kind of important bio-tissue structural protein, more and more causes people's concern and rise, and market potential is huge, economic worth is higher, particularly the collagen protein micromolecule active polypeptide, be widely used in medicine, beauty and health care and food service industry.The traditional technology that collagen protein extracts can be divided into five classes, i.e. acid system, alkaline process, enzyme process, salt method and hot water extraction etc.Its ultimate principle is all the characteristic according to collagen protein, changes the external environment of protein, and collagen protein is separated from other protein.Acid system and alkaline process are that collagen protein is extracted under acid and alkaline environment, and acid system is applied more extensive in the extraction process of collagen protein, and the alkaline process report seldom.The salt method is that raw material is always extracted at certain density salts solution, and the main salt used has sodium-chlor, Repone K and sodium acetate.Although above three kinds of method techniques are simple, cost is also low belongs to the high energy consumption industry owing in leaching process, can using a large amount of fresh water and acid-alkali salt, and the acid-alkali salt of discharge will produce certain ecological impact to surrounding environment.Do not meet green sustainability Economic Development Mode.The hot water extraction need to be used a large amount of fresh water and the energy, does not meet the main flow of country and development of world economy.
Biological enzyme is to utilize the gentle high efficiency of enzyme reaction collagen protein to be mentioned out from different raw materials under certain envrionment conditions, and the most frequently used enzyme has stomach en-, trypsinase, aspartic protease, Sumizyme MP, neutral protease and papoid at present.Along with the development of Enzymes Industry and constantly widening of Application Areas, zymin output increases rapidly, and market competition advantage constantly increases.The membrane separation concentration purification technique be the film that utilizes different pore size by target product according to its molecular size separated, the concentrated novel method of mentioning that is further purified, in production efficiency, energy consumption and aspect of performance have obvious advantage, in biological medicine, chemical industry and food service industry, be widely used, the film industrialization development is rapid at present in addition, auxiliary products are complete, and cost constantly reduces, and are the optimal selections of technique upgrading.
Summary of the invention
The present invention is directed to tuna skin material characteristic, by biological enzymolysis technology and scientific and reasonable being integrated of membrane technique, adopt industrial colloidal mill to carry out micronizing processing in early stage to raw material, by large flux high-speed and continuous centrifugal separation equipment, water-soluble noncollagen protein is separated, contain the precipitation of collagen protein after compound bio enzymolysis technology is hydrolyzed to the active small molecular polypeptide, after the film of special pore size distribution is separated concentrated the purification, obtain certain density active small molecular polypeptide slurries, obtain the high purity molecular weight and be less than 3KD collagen protein active small molecular polypeptide white powder finally by crossing the ultra micro drying of dusting.
The technical solution adopted for the present invention to solve the technical problems is: after raw material tuna skin is carried out to soaking and washing, at first use pulverizer to its carry out pulverization process for the fluid soup compound after, enter technical grade colloidal mill micronizing to 30～50 microns left and right of particle diameter, with enter 8000～12000 rev/mins of continuously centrifugeds of supercentrifuge after 2～6 times of volume water mix and separate the water-soluble substances of removing noncollagen protein in 30 minutes, enter the biological enzymolysis tank containing the water of the throw out of collagen protein and 3～5 times of volumes and carry out the complex enzyme hydrolysis processing.The prozyme chief component is trypsinase, papoid and Collagenase, addition is 0.5～2% of raw material, reaction conditions is 37～50 ℃ of temperature, PH7.5～8.0, in 2～4 hours reaction times, enzymatic vessel outlet is connected with membrane separation apparatus, ultra-filtration membrane through molecular weight, be 3KD, molecular weight be less than 3KD pass through after ultra-filtration membrane directly enter nanofiltration membrane and carry out after the desalination and concentration processing being dusted drying, the partial reflux that molecular weight is greater than 3KD continues enzymolysis to the biological enzymolysis tank.
The invention has the beneficial effects as follows: utilize to greatest extent enzyme and raw material to be worth, further reduce production costs, this technique invention has improved collagen protein extraction yield and purity, and wherein a large amount of water resources recycle, reduce production energy consumption.
Specific embodiments
Example 1: after raw material tuna skin is carried out to soaking and washing, at first use pulverizer to its carry out pulverization process for the fluid soup compound after, enter technical grade colloidal mill micronizing to 30 microns left and right of particle diameter, with enter 12000 rev/mins of continuously centrifugeds of supercentrifuge after 6 times of volume water mix and separate the water-soluble substances of removing noncollagen protein in 30 minutes, enter the biological enzymolysis tank containing the water of the throw out of collagen protein and 3 times of volumes and carry out the complex enzyme hydrolysis processing.The prozyme chief component is trypsinase, papoid and Collagenase, and addition is raw material 0.5%, and reaction conditions is 37 ℃ of temperature, PH8.0,2 hours reaction times.Enzymolysis product is processed with ultrafiltration apparatus, and the molecular weight that sees through of ultra-filtration membrane is 3KD, molecular weight be less than 3KD pass through after ultra-filtration membrane directly enter nanofiltration membrane and carry out after desalination and concentration being dusted drying, be finished product.The part that molecular weight is greater than 3KD turns back to biological enzymolysis tank continuation enzymolysis.
Example 2: after raw material tuna skin is carried out to soaking and washing, at first use pulverizer to its carry out pulverization process for the fluid soup compound after, enter technical grade colloidal mill micronizing to 50 microns left and right of particle diameter, with enter 8000 rev/mins of continuously centrifugeds of supercentrifuge after 2 times of volume water mix and separate the water-soluble substances of removing noncollagen protein in 30 minutes, enter the biological enzymolysis tank containing the water of the throw out of collagen protein and 5 times of volumes and carry out the complex enzyme hydrolysis processing.The prozyme chief component is trypsinase, papoid and Collagenase, and addition is raw material 2%, and reaction conditions is temperature 50 C, PH7.5,4 hours reaction times.Enzymolysis product is processed with ultrafiltration apparatus, and the molecular weight that sees through of ultra-filtration membrane is 3KD, molecular weight be less than 3KD pass through after ultra-filtration membrane directly enter nanofiltration membrane and carry out after desalination and concentration being dusted drying, be finished product.The part that molecular weight is greater than 3KD turns back to biological enzymolysis tank continuation enzymolysis.
Claims (4)
1. the preparation method of a tuna collagen small-molecular peptides, it is characterized in that the method includes the steps of: after raw material tuna skin is carried out to soaking and washing, at first after using pulverizer to carry out pulverization process to it, enter technical grade colloidal mill micronizing to 30～50 microns of particle diameters, with enter 8000～12000 rev/mins of continuously centrifugeds of whizzer after 2～6 times of volume water mix and separate and within 30 minutes, remove supernatant, the water of throw out and 3～5 times of volumes enters the biological enzymolysis tank and carries out enzymolysis processing, the prozyme addition is 0.5～2% of raw material, reaction conditions is 37～50 ℃ of temperature, PH7.5～8.0, 2～4 hours reaction times, enzymolysis product 3KD ultrafiltration membrane treatment, permeation parts is concentrated through nanofiltration membrane, spraying drying is former protein micromolecular peptide finished product.
2. a kind of preparation method of tuna collagen small-molecular peptides as claimed in claim 1, it is characterized in that the method includes the steps of: after raw material tuna skin is carried out to soaking and washing, at first after using pulverizer to carry out pulverization process to it, enter technical grade colloidal mill micronizing to 35～45 microns of particle diameters, with enter 9000～10000 rev/mins of continuously centrifugeds of whizzer after 3～5 times of volume water mix and separate and within 30 minutes, remove supernatant, the water of throw out and 3.5～4.5 times of volumes enters the biological enzymolysis tank and carries out enzymolysis processing, the prozyme addition is 1～1.5% of raw material, reaction conditions is 40～50 ℃ of temperature, PH7.5～8.0, 2.5～4 hours reaction times, enzymolysis product 3KD ultrafiltration membrane treatment, permeation parts is concentrated through nanofiltration membrane, spraying drying is former protein micromolecular peptide finished product.
3. a kind of preparation method of tuna collagen small-molecular peptides as claimed in claim 1, is characterized in that described prozyme chief component is trypsinase, papoid and Collagenase.
4. a kind of preparation method of tuna collagen small-molecular peptides as claimed in claim 1, it is characterized in that described nanofiltration membrane to see through molecular weight be 200D.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101730089A CN103421868A (en) | 2012-05-14 | 2012-05-14 | Preparation method of tuna skin collagen micro-molecular peptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101730089A CN103421868A (en) | 2012-05-14 | 2012-05-14 | Preparation method of tuna skin collagen micro-molecular peptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103421868A true CN103421868A (en) | 2013-12-04 |
Family
ID=49647259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101730089A Pending CN103421868A (en) | 2012-05-14 | 2012-05-14 | Preparation method of tuna skin collagen micro-molecular peptides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103421868A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105558257A (en) * | 2015-12-22 | 2016-05-11 | 潘爱国 | Method for extracting tuna small molecule peptides from tuna bones and application of tuna small molecule peptides |
| CN106191189A (en) * | 2016-09-20 | 2016-12-07 | 武汉百思凯瑞纳米科技有限公司 | A kind of preparation method and applications of tuna skin height moisturizing bioactive peptide |
| CN106319014A (en) * | 2016-10-14 | 2017-01-11 | 浙江双屿生物股份有限公司 | Extracting method for micro-molecule deep-sea fish polypeptides |
| CN107043800A (en) * | 2017-05-13 | 2017-08-15 | 广州固仑生物科技有限公司 | A kind of preparation method of collagen |
| CN112641084A (en) * | 2021-01-15 | 2021-04-13 | 平顶山天晶植物蛋白有限责任公司 | Preparation method of antifreeze soybean protein isolate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061827A (en) * | 2006-04-30 | 2007-10-31 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
-
2012
- 2012-05-14 CN CN2012101730089A patent/CN103421868A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061827A (en) * | 2006-04-30 | 2007-10-31 | 中国食品发酵工业研究院 | Industry method of producing fish collagen peptide from fish skin and bone by an enzyme method |
Non-Patent Citations (4)
| Title |
|---|
| 孙丹等: "骨胶生产工艺的改进-热水脱脂法", 《抚州石油学院学报》 * |
| 方健民等: "金枪鱼的营养价值和加工利用", 《安徽农业科学》 * |
| 郭恒斌等: "鱼皮胶原蛋白及胶原活性多肽的研究进展", 《食品与药品》 * |
| 郭玉华等: "鱼类胶原蛋白及胶原活性多肽的研究进展", 《中国食品添加剂》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105558257A (en) * | 2015-12-22 | 2016-05-11 | 潘爱国 | Method for extracting tuna small molecule peptides from tuna bones and application of tuna small molecule peptides |
| CN106191189A (en) * | 2016-09-20 | 2016-12-07 | 武汉百思凯瑞纳米科技有限公司 | A kind of preparation method and applications of tuna skin height moisturizing bioactive peptide |
| CN106319014A (en) * | 2016-10-14 | 2017-01-11 | 浙江双屿生物股份有限公司 | Extracting method for micro-molecule deep-sea fish polypeptides |
| CN107043800A (en) * | 2017-05-13 | 2017-08-15 | 广州固仑生物科技有限公司 | A kind of preparation method of collagen |
| CN112641084A (en) * | 2021-01-15 | 2021-04-13 | 平顶山天晶植物蛋白有限责任公司 | Preparation method of antifreeze soybean protein isolate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105063153B (en) | A kind of preparation method of food-grade low salt ocean fish oligopeptide powder | |
| CN101096696B (en) | Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process | |
| CN102174627B (en) | Method for preparing rapeseed bioactive peptide | |
| CN102010460B (en) | Tea seed polypeptide and preparation method thereof | |
| CN102308903B (en) | Industrialized production method of corn polypeptide | |
| CN103421868A (en) | Preparation method of tuna skin collagen micro-molecular peptides | |
| CN101664210B (en) | Processing method of sea cucumber egg nutrition | |
| CN101096697B (en) | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process | |
| CN105385737A (en) | Preparation technology of yak bone collagen oligopeptide | |
| CN101869169B (en) | Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology | |
| CN106319014A (en) | Extracting method for micro-molecule deep-sea fish polypeptides | |
| CN111154824A (en) | Industrial production method for obtaining spirulina antioxidant oligopeptide through high-concentration two-step enzymolysis | |
| CN103421871A (en) | Preparation method of tuna bone collagen peptide | |
| CN102887732B (en) | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias | |
| CN103421867A (en) | Preparation method of sea cucumber collagens | |
| CN101643766B (en) | Method for preparing hydrolyzed collagen protein by fresh animal skin | |
| CN103626836A (en) | Extraction process for polypeptide from waste liquid obtained in production of crude heparin sodium product | |
| CN103739663A (en) | Method for rapidly preparing small peptide amino acids by microwave-assisted protein acid hydrolysis | |
| CN105495658A (en) | Fish meal production system based on membrane treatment technology | |
| CN105441520A (en) | Method adopting rice residues as raw material for enzyme-membrane combined preparation of rice polypeptides | |
| CN105154506B (en) | A kind of food-grade low salt ocean fish oligopeptide powder and its application | |
| CN103421869A (en) | Preparation method of sea anemone low molecular polypeptides | |
| CN105154505B (en) | A kind of preparation method of feed grade ocean fish oligopeptide powder | |
| CN103421870A (en) | Preparation method of sea squirt low molecular polypeptides | |
| CN108101980B (en) | Preparation method of high-purity phycocyanin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131204 |
|
| RJ01 | Rejection of invention patent application after publication |