CN103421702A - Bacteria Lysinibacillus sp. for adsorbing gold and antimony - Google Patents
Bacteria Lysinibacillus sp. for adsorbing gold and antimony Download PDFInfo
- Publication number
- CN103421702A CN103421702A CN2012102178688A CN201210217868A CN103421702A CN 103421702 A CN103421702 A CN 103421702A CN 2012102178688 A CN2012102178688 A CN 2012102178688A CN 201210217868 A CN201210217868 A CN 201210217868A CN 103421702 A CN103421702 A CN 103421702A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- manganese
- antimony
- gold
- adsorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of environmental microorganism and relates to bacteria M14 having manganese oxidation capability and a use of the bacteria M14 in adsorption and recovery of gold. The bacterium M14 strain having soluble bivalent manganese oxidation capability is separated from manganese-polluted soil. The bacterium M14 strain can oxidize soluble bivalent manganese ions into water-insoluble manganese oxides and the water-insoluble manganese oxides can adsorb trivalent gold ions and trivalent antimony ions in water so that precious metal adsorption and recovery and heavy metal pollution treatment are realized. The bacterium M14 strain is names as Lysinibacillus sp. M14, belongs to a bacterial strain for producing manganese oxides, is preserved in the China center for type culture collection and has a preservation number of CCTCC NO: M2012084. A preliminary research proves that the bacterium M14 strain has a good application prospect in adsorption of heavy metal gold and toxic metal antimony in water.
Description
Technical field
The invention belongs to the environmental microorganism technical field, be specifically related to a strain and divalent manganesetion there is to the Mn oxide generation bacterium of oxygenizement, be the screening of Mn oxide generation bacterium (Lysinibacillus sp.) M14, the ability that its metabolism produces the biological manganese oxide compound, the present invention also comprises the purposes of this bacterium aspect absorption recovery heavy metal gold and oxidation-adsorption antimony.
Background technology
Heavy metal generally extensively is present in occurring in nature with natural concentration, but because the mankind are increasing to exploitation, smelting, processing and the business manufacturing activities of heavy metal, cause many waste liquids containing heavy metal as gold and silver, platinum, ruthenium, antimony etc., a large amount of waste liquids containing heavy metal are discharged, and have not only caused the waste of heavy metal resources also to pollute environment.Gold is a kind of very valuable metal, its mining deposits is very rare, and the content in the earth's crust approximately only accounts for 5/10000000000ths, and disperses very much, the price of gold is more and more expensive at present, so ADSORPTION OF GOLD becomes a kind of important channel of recycling noble metal from gold-containing solution.Antimony is a kind of toxic metal of extensive existence, in environment, mainly with positive trivalent and positive pentavalent form, exists, and antimony has chronic toxicity to biological and human body, serious also can carcinogenic (He Mengchang, Wan Hongyan, 2004), so the removing toxic substances of antimony is become and becomes more and more important.
Known at present, reclaiming golden main method from gold leachate has 1. method of cementation: the cementation technology mainly contains zinc dust precipitation and two kinds of methods of aluminium powder displacement.Although this method is very high to the golden rate of recovery, its cost is also very high simultaneously.2. active carbon adsorption: the charcoal adsorption technology adds gac referring in leaching vat or adsorption tank, with the adsorption of gac from ore pulp, adsorb reclaim golden.This method can be directly used in and reclaim gold from ore pulp, but the propagation and employment of this method is more difficult.3. resin adsorption method: gold can effectively be adsorbed and desorb on highly basic, highly basic and weak base hybrid resin, although this method has, the gold of carrying amount is high, the consumption advantage such as less, and its severe corrosive and toxicity, very easily cause secondary pollution.4. solvent extration: refer to that organic reagents such as utilizing alcohols, ethers, ketone, amine is as golden extraction agent, but its damage to operator's health is very big.5. electrodeposition metallurgy method: the leaching process of metal is in fact a redox reaction between metal and oxygenant, is that this leaching process is designed to a galvanic cell, but this method have cost high, easily cause the shortcomings such as secondary pollution, narrow application range.
And underground water at present commonly used has chemical precipitation method, electrochemical method, ion exchange method, chemosynthesis Mn oxide removal method etc. except the antimony method.Chemical precipitation method refers to that by additional chemical reagent etc., making antimony ion in water form mixture precipitates, to reach the purpose of removal.Electrochemical method refers to and allows acid waste water by being full of the cylindrical reactor of coke and iron filings, makes metal ion form simple substance and be detained as antimony, at negative electrode (coke), reduction reaction occurs, and then water outlet adds the alkali neutralization.Ion exchange method is to utilize the strong adsorptive power of ion exchange resin and activated alumina to carry out Adsorption of Heavy Metal Ions, reached more than 90 percent, but shortcoming is also fairly obvious, and as narrow application range, cost is high, and treatment capacity is little, easily causes secondary pollution etc.
In the previous work of the state Key Laboratory of Agricultural Microbiology at applicant place, the applicant has been found that a few strain manganese oxidizing bacterias have the effect (Wang Wenming such as very strong absorption, precipitation to various heavy (as As, Ni, Co, Cd, Zn etc.), 2008), and the biological manganese oxide compound can also be oxidized to the As of strong toxicity (III) then Adsorption of the weak As (V) of toxicity, therefore, the biological manganese oxide compound has very high application potential in heavy metal adsorption recovery and Heavy Metal Pollution Control.As the patent No. is the 2008101979019(denomination of invention: the brood cell bacillus MK 3-1 of purification of heavy metal pollution and purposes) patent documentation in relate to a strain manganese had to the adsorbing generation of oxidation bacterium MK3-1, this bacterium has certain effect to bivalent manganese oxidation and absorption, be mainly used in the oxidation-adsorption of divalent manganesetion, do not relate to the absorption to gold and antimony ion.Utilize at present the biological manganese oxide compound to the research of gold and the oxidation-adsorption of antimony seldom, the bacterial strain of the present invention's separation utilizes the biological manganese oxide compound of its generation to come ADSORPTION OF GOLD and antimony ion.
Summary of the invention
The object of the invention is to overcome the defect of prior art, separate and obtain the Mn oxide generation bacterium M14 that heavy metal gold and antimony are reclaimed in a strain absorption, this bacterial strain can be oxidized to insoluble Mn oxide by soluble divalent manganesetion, utilize this biological manganese oxide compound to carry out gold and the oxidation-adsorption antimony in adsorbent solution, can be applicable to the absorption of noble metal in solution or the water body of purification of heavy metal pollution.
The present invention is achieved through the following technical solutions:
The inventor separates, screens the Mn oxide generation bacterium M14 that heavy metal gold and antimony and manganese are reclaimed in a strain absorption from the topsoil sample of Tianjin City, China Xiqing District Malleable cast Iron plant manganese raw material silo, its Classification And Nomenclature Lysinibacillus sp, the applicant delivers this bacterial strain to China on March 15th, 2012. Hubei. and Chinese Typical Representative culture collection center (CCTCC) preservation in Wuhan, its preserving number is CCTCC NO:M2012084.
Mn oxide of the present invention produces the screening scheme techniqueflow shown in Figure 1 of bacterium M14.The isolate of described M14 bacterial strain, from the topsoil sample of Tianjin City, China Xiqing District Malleable cast Iron plant manganese raw material silo, adds finite concentration (detailed description seen below, lower same) MnCl
2Carry out enrichment culture, again the soil sample of enrichment culture is diluted and be coated with containing 20mmol/L hydroxyethyl croak piperazine second thiosulfonic acid (N '-a-hydroxythylpiperazine-N '-ethanesulfanic acid, be called for short HEPES, adjust pH to 7.0) and 0.3mmol/L MnCl
2K solid medium dull and stereotyped (reference literature: van Waasbergen, 1993), 28 ℃ of incubators are cultivated one week.Picking surface is the tan bacterium colony mono-clonal of ruling to obtain, use again LBB method (Krumbein et al., 1973) detect take and determine whether as manganese oxidation bacterium, again detected manganese oxidation bacterium is cooked the relevant evaluation work such as 16S ribosomal RNA gene (16S rDNA) mensuration, the Mn oxide that finally obtains a strain absorption and recovery heavy metal gold and antimony produces bacterium M14, and the applicant is by its called after Lysinibacillus sp.M14.
Positively effect of the present invention is:
At present, because the mankind are increasing to exploitation, smelting, processing and the business manufacturing activities of heavy metal, cause the waste liquid of the heavy metals such as many picture gold and antimony, not only caused the waste of resource also to cause serious environmental pollution.The biological manganese oxide compound that Lysinibacillus sp.M14 that separation screening of the present invention arrives produces can ADSORPTION OF GOLD ion and oxidation-adsorption antimony ion, thereby reaches the removal purpose of heavy metal.Therefore, the biological manganese oxidation has very high application potential in the improvement of the absorption recovery of noble metal and heavy metal contamination with in repairing.
The comparison of the present invention and the applicant's previous work, its contrast effect is as described in Table 1.
Table 1 the present invention is that 2008101979019 documents are compared the positively effect had with the patent No.
More detailed technological step is shown in " embodiment " described (Liu Yanjun, Zhou Jingxiao, Wang Gejiao, 2009).
The accompanying drawing explanation
Sequence table SEQ ID NO:1 is the nucleotide sequence that the present invention separates (Lysinibacillus sp.) M14 genome 16S rDNA gene.
Fig. 1: be separation, the triage techniques schema that Mn oxide of the present invention produces bacterium (Lysinibacillus sp.) M14.
Fig. 2: be the LBB detected result that Mn oxide of the present invention produces bacterium (Lysinibacillus sp.) M14 bacterium colony.In figure: bacterial strain M14 is containing MnCl
2The K substratum on cultivate the colonial morphology after 4d: brown is the visual inspection colony colour; Sapphirine is the colony colour that the LBB finger-length measurement detects.
Fig. 3: be the scanning electron microscope picture that Mn oxide of the present invention produces bacterium (Lysinibacillus sp.) M14 somatic cells.
Fig. 4: be the design sketch that Mn oxide of the present invention produces bacterium (Lysinibacillus sp.) M14 oxidation removal manganese.In figure: manganese oxidation, the removal curve mark of M14 bacterium to Mn (II): ◆, Mn (II) removes curve; █, Mn (II) adsorption curve; ▲, Mn (II) oxidation curve; Zero, blank.
Fig. 5: be that Mn oxide of the present invention produces the design sketch that bacterium (Lysinibacillus sp.) M14 produces thing Mn oxide ADSORPTION OF GOLD.Mn oxide and the thalline adsorption curve mark to Au (III): ■ in figure, add after biological manganese the change curve of Au (III) concentration in supernatant liquor; ●, blank, do not add any Mn oxide, and the starting point concentration of Au (III) is 200 μ M.
Fig. 6: be that Mn oxide of the present invention produces the design sketch that bacterium (Lysinibacillus sp.) M14 produces thing Mn oxide oxidation-adsorption antimony.The biological manganese oxide compound is to mark: ■ in the oxidation-adsorption curve of Sb (III), blank, and initial Sb (III) concentration is 30 μ M, without any Mn oxide, solvent is deionized water; ▲, add after biological manganese the change curve of Sb (III) concentration in supernatant liquor; ◆, add after biological manganese the change curve of Sb (V) concentration in supernatant liquor.
Embodiment
Embodiment 1: separate from soil polluted by manganese and identify Lysinibacillus sp.M14
(1) sample is taked: in late June, 2007 collects the wad sample of this test from the topsoil of Tianjin City, China Xiqing District Malleable cast Iron plant manganese raw material silo.
(2) example enrichment: get the soil sample of 100g, in soil sample, add aseptic MnCl
2It is 989.55mg/Kg that solution makes its final concentration, stirs evenly gently to put in 28 ℃ of incubators to cultivate one week, notes adding sterilized water, guarantees Sample moisture.
(3) manganese oxidation bacterium separates: accurately take through MnCl
2The soil sample 10g of enrichment, in the triangular flask that the 90ml stroke-physiological saline solution is housed, puts in 28 ℃ of shaking tables and vibrates half an hour, then gets successively 1ml and join in the 9ml stroke-physiological saline solution and progressively be diluted to 10
-2, 10
-3, 10
-4, get respectively 0.1ml coating containing 20mmol/L hydroxyethyl croak piperazine second thiosulfonic acid (N '-a-hydroxythylpiperazine-N '-ethanesulfanic acid, be called for short HEPES, adjust pH to 7.0) and 0.3mmol/L MnCl
2K solid medium dull and stereotyped (van Waasbergen et al., 1993), 3 flat boards of each extent of dilution coating, put in 28 ℃ of incubators and cultivate one week, by surface, is that tan bacterium colony is decided to be doubtful manganese oxidation bacterium, by flat board, puts in 4 ℃ of refrigerators stand-by.Above-mentioned K solid culture based formulas is as follows: yeast extract paste 0.5g, peptone 2g, agar 15g, 1L artificial seawater (1L artificial seawater: NaCl 13.14g, KCl 0.56g, MgSO
47H
2O 9.24g, CaCl
20.83g).
(4) line separates: the bacterium colonies that the doubtful manganese oxidation bacterium picking obtained in step (3) is different are rule, guarantee to obtain mono-clonal, the K culture medium flat plate use in line, treats that bacterium grows be placed on stand-by in 4 ℃ of refrigerators and preserve portion in-80 ℃ of refrigerators with the Freezing Glycerine pipe.
(5) bacterial strain manganese oxidizing property is identified: the mono-clonal obtained in step (4) is transferred to containing 20mmol/L HEPES (pH 7.0) and 0.3mmol/LMnCl
2In K solid medium flat board, put in 28 ℃ of incubators and cultivate, when dull and stereotyped upper generation brown bacterium colony, by Berbelin blue-I (LBB, N.N'-Dimethylamino-p, p'-triphenyhnethane-o " sulphonicacid) drop on the brown bacterium colony and detect its oxidisability.If solution colour does not change and does not illustrate that this bacterium does not have the manganese oxidisability, if color is become dark sapphirine and illustrated the manganese oxidisability is arranged this bacterium (see Fig. 3, LBB is the staining agent of redox agent, Mn by light blue
3+/4++ LBB (reduced)>Mn
2++ LBB (oxidized), oxidized LBB is dark sapphirine).The compound method of LBB is as follows: with 4 ℃ of dark places of 40mmol/L aqueous acetic acid of 100ml, spend the night and dissolve the LBB of 40mg.
(6) classification of manganese oxidation bacterium is identified: use colony polymerase chain reaction (PCR) method amplification 16S rDNA: mix in 50 μ L aseptic double-distilled waters with the monoclonal bacterial strain of toothpick picking; 100 ℃ of thermal shock 5min,-20 ℃ of 5min, cold and hot hit repetition 2 ~ 3 times after, the centrifugal 3min of 12000r/min, the supernatant liquor obtained directly is used as the pcr amplification template, amplification system is 50 μ L systems, universal primer: 27 (5'AGAGTTTGATCMTGGCTCAG3 ') and 1492R (5'GGYTACCTTGTTACGACTT3 ').Amplification condition is: denaturation, 95 ℃, 5min; Sex change, 94 ℃, 45sec; Annealing, 49 ℃, 45sec; Extend, 72 ℃, 1.5min sex change-extension, 35 circulations; Extend: 72 ℃, 10min.Obtain the nucleotide sequence as shown in sequence table SEQ ID NO:1, sequence length is 1395bp.The agarose gel electrophoresis that is 1% by concentration detects the PCR product.The pillar DNA produced with the Shanghai Cyberway gene engineering company limited bought reclaims test kit to the PCR product purification, operation to specifications, and the three rich polygala root companies that deliver to are checked order.By existing sequence in the 16S rDNA gene order (SEQ ID NO:1 is shown in sequence table) that obtains and GenBank library compare (comparison result is shown in Fig. 1).
The Mn oxide that the present invention separates produces the mycology feature of bacterium (Lysinibacillus sp.M14):
The thalline rod-short, gram-positive microorganism, suitable growth temperature 28-30 ℃, appropriate pH 7.0-7.5, do not containing MnCl
2The K solid medium on, bacterium colony is circular, oyster white, projection, surface wettability, edge is irregular.At the MnCl containing 0.3mmol/L
2On the K substratum, the bacterium colony surface is the brown (see figure 2); This bacterium scanning electron microscope (SEM) photograph is shown in Fig. 3.
The method for preserving of Lysinibacillus sp.M14 is as follows:
Lysinibacillus sp.M14 can, in the upper 28 ℃ of cultivations of K liquid or solid substratum (filling a prescription the same), can make short term storage after cultivation under 4 ℃.If long-term preservation, can be used Freezing Glycerine pipe or lyophilize pipe (Zhao Bin, He Shaojiang, 2002) this bacterial strain of preservation proper.
Embodiment 2: Mn oxide produces the manganese oxidation removal curve of bacterium (Lysinibacillus sp.) M14
Prepare 3 bottles of sterilized liquid K substratum (filling a prescription the same), every bottle of 100mL.Add the 20mmol/L hydroxyethyl croak piperazine second thiosulfonic acid (HEPES) of filtration sterilization, add the aseptic MnCl of 1mol/L simultaneously
2It is 0.3mmol/L that mother liquor makes final concentration.Then inoculate (the OD of logarithmic phase
600Value is about 0.7) M14 bacterium liquid, 1% inoculum size inoculation by volume.Sample immediately 10mL after mixing, this sample, as sampling first, is placed in clean centrifuge tube.Each triangular flask is placed in to 28 ℃, and in the 160r/min shaking table, concussion is cultivated.After this sampling interval is 24h, and this experiment arranges three repetitions.After sampling finishes, sample is done following the processing:
1) the 10mL sample of at every turn obtaining, with 8000r/min high speed centrifugation 6min, retain supernatant liquor, the water system membrane filtration that is 0.22 μ m with aperture, and filtrate is placed in-20 ℃ of preservations.The applicant is referred to as " remaining Mn (II) " by the Mn (II) existed in this part solution.
2) the centrifugal precipitation obtained is processed by the following method: after centrifugal with 3 washings of deionized water repetition, add the CuSO that 4mL concentration is 0.1mol/L
4Solution, break up thalline with pipettor, with the velocity fluctuation 12h of 160r/min.Then take out, the bacteria suspension in pipe is settled to 10mL with deionized water, and 8000r/min high speed centrifugation 6min retains supernatant liquor, the water system membrane filtration that is 0.22 μ m with aperture, and filtrate is placed in-20 ℃ of preservations.The applicant is referred to as " Mn of absorption (II) " by the Mn (II) existed in this part solution.
3) remaining precipitation is done following processing: after again with deionized water, repeating 3 washings, add 4mL oxammonium hydrochloride mother liquor (0.1mol/L), with pipettor, thalline is broken up, with the velocity fluctuation 12h of 160r/min.Then take out, the bacteria suspension in pipe is settled to 10mL with deionized water, after 8000r/min high speed centrifugation 5min, retains supernatant liquor, the water system membrane filtration that is 0.22 μ m with aperture, and filtrate is placed in-20 ℃ of preservations.The applicant is referred to as " Mn of oxidation (II) " by the Mn (II) existed in this part solution.
By the concentration of Mn contained in the flame atomic absorption spectrophotometer working sample (II), Mn (II) concentration of the sample that records of take is ordinate zou, take the time as the X-coordinate mapping.
In oxidation removal experiment the M14 bacterium to Mn (II), because manganese oxidation bacterium can produce stress reaction to the Mn (II) to concentration, secretory protein class material, think the trend of understanding better this bacterial strain oxidation removal Mn (II), the Mn of selection (II) starting point concentration is 30mM.The graphic representation that Fig. 4 is M14 bacterium oxidation removal Mn (II), as we can see from the figure, As time goes on the M14 bacterial strain that the present invention separates continues to increase the clearance of manganese, especially when 3 ~ 7d, removing speed the fastest, may be because bacterial strain during this starts the Mn (II) in oxidizing solution, produce Mn oxide, when 10d, " remaining Mn (II) " concentration reaches minimum, and clearance reaches 92.62%.While starting from reaction, (II) is attracted to thalline or Mn oxide surface to start that Mn is arranged, and is continuous increase tendency before 5d, but decrease after 5d, may be to have the Mn (II) partly be adsorbed oxidized, final, " Mn of absorption (II) " concentration be about 0.04mM.The applicant finds, manganese oxidation curve is always in continuous ascendant trend, when 3 ~ 7d, in solution, remaining Mn (II) constantly reduces, but " Mn of oxidation (II) " constantly increase, and the Mn (II) adsorbed does not increase considerably, illustrate that most Mn (II) is oxidized, when 10d, the M14 bacterium oxidation efficiency that the present invention separates reaches the highest, is about 82.66% (seeing Fig. 4).
Embodiment 3:Lysinibacillus sp.M14 produces the mensuration of thing Mn oxide ADSORPTION OF GOLD ability in gold-containing solution
Lysinibacillus sp.M14 thalline of the present invention is inoculated in to the Mn containing 5mM
2+K substratum (filling a prescription the same) in, be placed in 28 ℃, cultivate 10d in the shaking table of 150r/min, the substratum total amount is 3000mL, after having the brownish black precipitation to generate in substratum, with 8000r/min high speed centrifugation culture, remove supernatant, will be deposited in-56 ℃ of lower lyophilizes, grinding, cross 100 mesh sieves.The biological manganese oxide compound of 1.87g is joined in the triangular flask that the 100mL distilled water is housed, triangular flask needs to wrap black plastic and sees phototropic to prevent Au (III) before experiment, the hydrochloro-auric acid that adds 200 μ L 100mmol/L after shaking up, mix rear sampling 3mL as sampling first.Each triangular flask is placed in to 28 ℃, and in the shaking table of 150r/min, front 1h, get sample one time every 0.5h, gets a sample centrifuging every 1h thereafter.Sampling 6h, each 3mL suspension taken out, at 8000r/min high speed centrifugation 5min, retains supernatant liquor, the water system membrane filtration that is 0.22 μ m with aperture, filtrate is placed in clean 5ml centrifuge tube, and-20 ℃ are frozen to be detected.After sampling finishes, with flame atomic absorption spectrophotometer, measure residue Au (III) concentration.
Take the time as X-coordinate, and Au (III) concentration is the ordinate zou mapping, draws the change curve (see figure 5) of remaining Au (III) in biological manganese oxide compound ADSORPTION OF GOLD system.
The present embodiment is established two reaction systems, be respectively blank and biological manganese oxide compound absorption system, each reaction system is tested by the described method of embodiment 3, after the Mn oxide collection that the M14 bacterium generates, pre-treatment, the adsorptive power of Au (III) is shown in to Fig. 5.
In blank assay, do not add any Mn oxide in gold-containing solution, the starting point concentration of Au (III) is 200 μ M, in 6h, the concentration of As in solution (III) is all the time in than steady state, but reduced approximately 21.53 μ M Au (III) than starting point concentration, analysis may be to be adsorbed by other means, as the triangular flask bottle.
The biological manganese oxide compound produces the thing Mn oxide to Au (III) absorption system by 100ml distilled water, 200 μ M Au (III) and 1.87g M14 bacterium and forms.In incipient 0.5h, Au (III) adsorptive capacity fast rise, after 1h, the biological manganese oxide compound reaches balance to the absorption of Au (III), and adsorption efficiency approaches 100%.
Embodiment 4:Lysinibacillus sp.M14 produces the mensuration of thing Mn oxide oxidation-adsorption antimony ability in antimony solution
Utilize the method described in embodiment 3 cultivate the biological manganese oxide compound and it is processed, the biological manganese oxide compound of 1.87g is joined in the triangular flask that the 100mL distilled water is housed, the antimonypotassium tartrate that adds 30 μ L 100mmol/L after shaking up, mix rear sampling 1mL as sampling first, experiment arranges three repetitions.Triangular flask is placed in to 28 ℃, in the shaking table of 150r/min, 0.5h the time get sample one time, every 2h get once thereafter, sampling 12h, each 1mL suspension taken out is at 8000r/min high speed centrifugation 5min, retain supernatant liquor, the water system membrane filtration that is 0.22 μ m with aperture, filtrate is placed in clean 2ml centrifuge tube, and-20 ℃ are frozen to be detected.After sampling finishes, with high performance liquid chromatography-hydride Generation-Atomic Fluorescence Spectrometry HPLC-HG-AFS (High performance liquid chromatography hydride generation atomic fluorescence spectrometry), measure residue Sb (III) and Sb (V) concentration.
After the Mn oxide collection that the M14 bacterium generates, pre-treatment, the oxidation-adsorption ability of Sb (III) is shown in to Fig. 6.In biological manganese oxide compound oxidation-adsorption Sb (III) system, Sb in supernatant liquor (III) concentration descends rapidly in 30min, be reduced to 6.5 μ M from initial 30 μ M, thereafter in 6h in supernatant liquor Sb (III) concentration be reduced to 0 μ M, Sb (III) is completely removed.The minimizing of Sb (III) is accompanied by the generation of Sb (V), but the generation of Sb (V) is slow in the minimizing of Sb (III), Sb (III) concentration has decline process sharply in 0 ~ 6h, substantially Sb (V) do not detected in supernatant liquor in this time period, after 6h, Sb in supernatant liquor (V) concentration just rises to some extent, but concentration is also very low, illustrate that most Sb has been adsorbed by the biological manganese oxide compound, comprise Sb (III) and Sb (V).
In blank assay, do not add any Mn oxide containing in antimony solution, in 12h, the concentration of Sb in solution (III) remains stable, but reduced approximately 7 μ M Sb (III) than starting point concentration, analysis may be to be adsorbed by other means, as the absorption of triangular flask bottle.
Produce in the oxidation-adsorption experiment of thing Mn oxide to antimony and find at M14, the biological manganese oxide compound can effectively be removed the Sb (III) of 30 μ M in 6h, has reached the detoxification to antimony.
Reference
1. He Mengchang, Wan Hongyan. the distribution of antimony in environment, there are form and toxicity and biological effectiveness. chemical progress, 2004,16 (1): 131-135
2. Liu Yan army, Zhou Jingxiao, Wang Gejiao. Mn (II) oxidation characteristic and the demanganization capability study of manganese oxidation bacterium Bacillus sp.MK3-1. microbiology circular, 2009,36 (4): 1-6
3. king's civilization. deep-sea manganese oxidizing bacteria Brachybacterium sp.Mn32 is the absorption research .[master thesis to Zn (II), Ni (II) to the oxidation of Mn (II) and biological manganese oxide compound]. Wuhan: Hua Zhong Agriculture University Library, http://www.cnki.net/kcms/detail/detail.aspx dbcode=CMFD& QueryID=0& CurRec=18& Dbname=CMFD0911& Filename=2008203105.nh& Uid=WEEvREcwSlJHSldTTGJhYlN2V1pHa3VQdHhQTXNLZ0dJcCtidUhx Y1hJUHdRS0N1M0x1NTdZN3NaMVpvWmRROA==, 2008
4. Zhao Bin, He Shaojiang. Microbiology Experiment. first version. the .2002:202-205 of Science Press
5.Krumbein?W?E?et?al.A?new?method?for?the?detection?and?enumeration?of?manganese?oxidizing?and?reducing?microorganisms.Helgoland?Marine?Research,1973,25:347-356
6.van?Waasbergen?L?G,Hildebrand?M,Tebo?B?M.Identification?and?characterization?of?a?gene?cluster?involved?in?manganese?oxidation?by?spores?of?the?marine?Bacillus?sp.strain?SG-1.Journal?of?Bacteriology,1996,178(12):3517-3530
Claims (3)
1. a Mn oxide that adsorbs and reclaim heavy metal gold and antimony produces bacterium Lysinibacillus sp.M14, is deposited in Chinese Typical Representative culture collection center (CCTCC), and its preserving number is CCTCC NO:M2012084.
2. the application of bacterial strain claimed in claim 1 in absorption and recovery heavy metal gold and antimony.
3. application claimed in claim 1, also be included in absorption and reclaim the application in the heavy metal manganese oxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210217868.8A CN103421702B (en) | 2012-06-28 | 2012-06-28 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210217868.8A CN103421702B (en) | 2012-06-28 | 2012-06-28 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103421702A true CN103421702A (en) | 2013-12-04 |
| CN103421702B CN103421702B (en) | 2015-03-11 |
Family
ID=49647097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210217868.8A Expired - Fee Related CN103421702B (en) | 2012-06-28 | 2012-06-28 | Bacteria Lysinibacillus sp. for adsorbing gold and antimony |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103421702B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244501A (en) * | 2016-09-23 | 2016-12-21 | 北京林业大学 | One strain anti-antimony antibacterial NXH1 and application thereof |
| CN106434446A (en) * | 2016-09-23 | 2017-02-22 | 北京林业大学 | Anti-antimony bacteria NXH3 and application thereof |
| CN109440479A (en) * | 2018-12-04 | 2019-03-08 | 浪莎针织有限公司 | A kind of method that bioleaching removes heavy metal antimony in polyester fiber fabric |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101397544A (en) * | 2007-09-30 | 2009-04-01 | 中国科学院生态环境研究中心 | Manganese oxidizing bacteria bacillus strain WH4 and use thereof |
| CN101429486A (en) * | 2008-11-27 | 2009-05-13 | 华中农业大学 | Brood cell bacillus MK3-1 for purification of heavy metal pollution and uses thereof |
| CN102827786A (en) * | 2012-04-18 | 2012-12-19 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater |
-
2012
- 2012-06-28 CN CN201210217868.8A patent/CN103421702B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101397544A (en) * | 2007-09-30 | 2009-04-01 | 中国科学院生态环境研究中心 | Manganese oxidizing bacteria bacillus strain WH4 and use thereof |
| CN101429486A (en) * | 2008-11-27 | 2009-05-13 | 华中农业大学 | Brood cell bacillus MK3-1 for purification of heavy metal pollution and uses thereof |
| CN102827786A (en) * | 2012-04-18 | 2012-12-19 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. for degrading S<2-> in petrochemical wastewater |
Non-Patent Citations (4)
| Title |
|---|
| CORINNE R.LEHR等: "New Insights into Microbial Oxidation of Antimony and Arsenic", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》, vol. 73, no. 7, 31 December 2007 (2007-12-31), pages 2386 - 2389 * |
| MINYAN HE等: "Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1", 《JOURNAL OF HAZARDOUS MATERIALS》, vol. 185, 29 September 2010 (2010-09-29), pages 682 - 688 * |
| ULRIKE JANKOWSKI等: "S-Layer protein from Lysinibacillus sphaericus JG-A12 as matrix for AuIII sorption and Au-nanoparticle formation", 《SPECTROSCOPY》, vol. 24, 31 December 2010 (2010-12-31), pages 177 - 181 * |
| 王惠 等: "利用赖氨酸芽孢杆菌产生的生物锰氧化物吸附和回收金离子", 《华中农业大学学报》, vol. 32, no. 5, 30 September 2013 (2013-09-30), pages 29 - 33 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244501A (en) * | 2016-09-23 | 2016-12-21 | 北京林业大学 | One strain anti-antimony antibacterial NXH1 and application thereof |
| CN106434446A (en) * | 2016-09-23 | 2017-02-22 | 北京林业大学 | Anti-antimony bacteria NXH3 and application thereof |
| CN106434446B (en) * | 2016-09-23 | 2019-09-17 | 北京林业大学 | One plant of anti-antimony bacterium NXH3 and its application |
| CN109440479A (en) * | 2018-12-04 | 2019-03-08 | 浪莎针织有限公司 | A kind of method that bioleaching removes heavy metal antimony in polyester fiber fabric |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103421702B (en) | 2015-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Construction of fungi-microalgae symbiotic system and adsorption study of heavy metal ions | |
| Gerber et al. | Metabolism-dependent bioaccumulation of uranium by Rhodosporidium toruloides isolated from the flooding water of a former uranium mine | |
| CN108865908B (en) | High-antimony-resistance rhodotorula mucilaginosa DJHN070401, and separation and screening method and application thereof | |
| CN109967519B (en) | Microbial remediation method for heavy metal pollution of tailing pond | |
| CN104531585A (en) | Delftia tsuruhatensis and application thereof | |
| Arakawa et al. | Growth stimulation of iodide-oxidizing α-Proteobacteria in iodide-rich environments | |
| Lin et al. | Screening and identification of Lactic acid bacteria from Ya’an pickle water to effectively remove Pb 2+ | |
| CN109759436A (en) | The method of this category bacterium bacterial strain of the Providian of one plant of resistance to manganese and restoration of soil polluted by heavy metal | |
| CN103421702B (en) | Bacteria Lysinibacillus sp. for adsorbing gold and antimony | |
| CN103740623A (en) | Pseudoalteromonas sp. capable of efficiently removing cadmium and phosphorus in wastewater and application thereof | |
| Fosso-Kankeu et al. | A comprehensive study of physical and physiological parameters that affect bio-sorption of metal pollutants from aqueous solutions | |
| Miyashita et al. | Recovery of Au from dilute aqua regia solutions via adsorption on the lyophilized cells of a unicellular red alga Galdieria sulphuraria: A mechanism study | |
| CN109504625B (en) | Bacillus cereus DIF1 and microbial agent produced by same and application of bacillus cereus DIF1 | |
| CN103833144B (en) | A kind of method utilizing flcos producing bacteria fermented liquid to remove heavy metal ion in water | |
| CN109574407B (en) | Method for efficiently removing butyl xanthate and heavy metals Cr (VI) in flotation wastewater by biological method | |
| Abu-Mejdad | Response of some fungal species to the effect of copper, magnesium and zinc under the laboratory condition | |
| CN103740593B (en) | A kind of anabena and application thereof of energy degradation of polychlorinated biphenyl | |
| CN104332645A (en) | Microbiological fuel cell for processing lead-containing sewage | |
| CN107164239B (en) | Purple lilac spore bacterium and method for repairing heavy metal in polluted water body by using purple lilac spore bacterium and synergistic biomass | |
| Wang et al. | Arsenic binding to iron (II) minerals produced by An iron (III)‐Reducing Aeromonas strain isolated from paddy soil | |
| Ghazvini et al. | Effect of salinity on vanadate biosorption by Halomonas sp. GT-83: Preliminary investigation on biosorption by micro-PIXE technique | |
| Zhang et al. | Biosorption of copper and nickel ions using Pseudomonas sp. in single and binary metal systems | |
| Ghazvini et al. | Screening of bacterial cells for biosorption of oxyanions: Application of micro-PIXE for measurement of biosorption | |
| CN102229896A (en) | Application of radiation resistant bacteria in biological treatment of heavy metal ions | |
| CN104560812A (en) | Facultative anaerobic paracoccus arsenic oxydans and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150311 Termination date: 20200628 |