CN103418027A - Preparation method of composite porous scaffold material - Google Patents
Preparation method of composite porous scaffold material Download PDFInfo
- Publication number
- CN103418027A CN103418027A CN2013103275393A CN201310327539A CN103418027A CN 103418027 A CN103418027 A CN 103418027A CN 2013103275393 A CN2013103275393 A CN 2013103275393A CN 201310327539 A CN201310327539 A CN 201310327539A CN 103418027 A CN103418027 A CN 103418027A
- Authority
- CN
- China
- Prior art keywords
- composite porous
- preparation
- porous support
- solution
- support material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a preparation method of a composite porous scaffold material, which comprises the following steps: treating natural silk by using a weak base solution at the temperature of 75-85 DEG C for 30-40 minutes, cleaning, and then drying so as to obtain fibroin fiber; dissolving the fibroin fiber, adjusting the pH value of the solution to reach 8.0-8.5, standing, then adding nano-hydroxyapatite, uniformly stirring, and performing aftertreatment so as to obtain the composite porous scaffold material. Through the mode, the porosity of the composite porous scaffold material prepared through the preparation method of the composite porous scaffold material, disclosed by the invention, reaches 78-85%, the material has the same pore diameter as that of the material of the bone tissue engineering, and the interface bonding of the material is excellent; the preparation method has the advantages that the operation is simple, the used raw material is low in cost, in the preparation process, the effects of environmental protection and no-pollution are realized, and the large-scale application of the obtained material can be realized.
Description
Technical field
The present invention relates to field of compound material, particularly relate to a kind of preparation method of composite porous support material.
Background technology
Bone tissue engineer refers to autologous high concentration osteoblast, bone marrow stroma stem cell or the chondrocyte that will separate, after cultured and amplified in vitro, plant in a kind of natural or synthetic, have good biocompatibility, can by human body progressively degraded and absorbed cytoskeleton or claim on extracellular matrix.This biomaterial scaffolds can be the three dimensions that cell provides existence, be conducive to cell and obtain enough nutrient substance, carry out gas exchange, get rid of waste material, cell is grown on the three-dimensional rack of prefabricated form, then by this cell hybrid material implantable bone defect, when biomaterial is progressively degraded, the osteocyte of plantation is constantly bred, thereby reach, repairs the damaged purpose of osseous tissue.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of preparation method of composite porous support material, the composite porous support material function admirable that the method makes.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of preparation method of composite porous support material is provided, comprises that step is:
(1) silkworm silk is processed to 30-40 minute with weak caustic solution under 75-85 ℃, clean post-drying obtains fibroin fiber;
(2) described fibroin fiber is dissolved and the regulator solution pH value is 8.0-8.5, add nanometer hydroxyapatite and stir after standing, post processing obtains composite porous support material.
In a preferred embodiment of the present invention, described in step (1), weak caustic solution is solution of potassium carbonate, sodium bicarbonate solution or sodium carbonate liquor.
In a preferred embodiment of the present invention, the solution of regulating pH value described in step (2) is potassium carbonate or sodium carbonate.
In a preferred embodiment of the present invention, described in step (2), time of repose is 20-22 hour.
The invention has the beneficial effects as follows: the preparation method of composite porous support material of the present invention, the porous compound support frame material porosity made reaches 78-85%, there is the aperture identical with bone tissue engineer, the interface of material is in conjunction with good, this preparation method is simple to operate, the cost of material cheapness of using, preparation process is environment friendly and pollution-free, the material energy large-scale application obtained.
The specific embodiment
Below will the technical scheme in the embodiment of the present invention be clearly and completely described, obviously, described embodiment is only a part of embodiment of the present invention, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making all other embodiment that obtain under the creative work prerequisite, belong to the scope of protection of the invention.
Embodiment mono-:
A kind of preparation method of composite porous support material is provided, comprises that step is:
(1) silkworm silk is processed 30 minutes under 75 ℃ with sodium bicarbonate solution, clean post-drying obtains fibroin fiber;
(2) described fibroin fiber being dissolved and use the sodium carbonate regulating solution pH value is 8.5, after standing 21 hours, adds nanometer hydroxyapatite and stirs, and post processing obtains composite porous support material.
Embodiment bis-:
A kind of preparation method of composite porous support material is provided, comprises that step is:
(1) silkworm silk is processed 40 minutes under 85 ℃ with sodium carbonate liquor, clean post-drying obtains fibroin fiber;
(2) described fibroin fiber being dissolved and use potassium carbonate regulator solution pH value is 8.3, after standing 20 hours, adds nanometer hydroxyapatite and stirs, and post processing obtains composite porous support material.
Embodiment tri-:
A kind of preparation method of composite porous support material is provided, comprises that step is:
(1) silkworm silk is processed 35 minutes under 80 ℃ with solution of potassium carbonate, clean post-drying obtains fibroin fiber;
(2) described fibroin fiber being dissolved and use the sodium carbonate regulating solution pH value is 8.0, after standing 22 hours, adds nanometer hydroxyapatite and stirs, and post processing obtains composite porous support material.
The beneficial effect of the preparation method of composite porous support material of the present invention is: the porous compound support frame material porosity made reaches 78-85%, there is the aperture identical with bone tissue engineer, the interface of material is in conjunction with good, this preparation method is simple to operate, the cost of material cheapness of using, preparation process is environment friendly and pollution-free, the material energy large-scale application obtained.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical field, all in like manner be included in scope of patent protection of the present invention.
Claims (4)
1. the preparation method of a composite porous support material, is characterized in that, comprises that step is:
(1) silkworm silk is processed to 30-40 minute with weak caustic solution under 75-85 ℃, clean post-drying obtains fibroin fiber;
(2) described fibroin fiber is dissolved and the regulator solution pH value is 8.0-8.5, add nanometer hydroxyapatite and stir after standing, post processing obtains composite porous support material.
2. the preparation method of composite porous support material according to claim 1, is characterized in that, described in step (1), weak caustic solution is solution of potassium carbonate, sodium bicarbonate solution or sodium carbonate liquor.
3. the preparation method of composite porous support material according to claim 1, is characterized in that, the solution of regulating pH value described in step (2) is potassium carbonate or sodium carbonate.
4. the preparation method of composite porous support material according to claim 1, is characterized in that, described in step (2), time of repose is 20-22 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103275393A CN103418027A (en) | 2013-07-31 | 2013-07-31 | Preparation method of composite porous scaffold material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103275393A CN103418027A (en) | 2013-07-31 | 2013-07-31 | Preparation method of composite porous scaffold material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103418027A true CN103418027A (en) | 2013-12-04 |
Family
ID=49643761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103275393A Pending CN103418027A (en) | 2013-07-31 | 2013-07-31 | Preparation method of composite porous scaffold material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103418027A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105688283A (en) * | 2016-02-01 | 2016-06-22 | 苏州大学 | Modified silk fibroin-calcium phosphate composite bone cement and preparation method thereof |
| CN108404208A (en) * | 2018-05-25 | 2018-08-17 | 姜香 | A kind of preparation method of wear-resistant type artificial joint material |
| CN108914241A (en) * | 2018-07-20 | 2018-11-30 | 广西科技大学 | A kind of preparation method of quickly controllable silk fibroin staple fiber |
| CN110180029A (en) * | 2019-05-16 | 2019-08-30 | 暨南大学 | A kind of preparation method and application with induced osteogenesis differentiation and the degradation material of bone regeneration function |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008103017A1 (en) * | 2007-02-23 | 2008-08-28 | Pusan National University Industry-University Cooperation Foundation | Biodegradable porous composite and hybrid composite of biopolymers and bioceramics |
| CN101703795A (en) * | 2009-11-11 | 2010-05-12 | 复旦大学附属中山医院 | Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material |
| CN101773684A (en) * | 2010-03-19 | 2010-07-14 | 西南大学 | Preparation method of fibroin/hydroxyapatite porous scaffold |
| KR20110040388A (en) * | 2009-10-14 | 2011-04-20 | 서울대학교산학협력단 | Silk/hydroxyapatite hybrid nanofiber scaffold for bone regeneration |
-
2013
- 2013-07-31 CN CN2013103275393A patent/CN103418027A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008103017A1 (en) * | 2007-02-23 | 2008-08-28 | Pusan National University Industry-University Cooperation Foundation | Biodegradable porous composite and hybrid composite of biopolymers and bioceramics |
| KR20110040388A (en) * | 2009-10-14 | 2011-04-20 | 서울대학교산학협력단 | Silk/hydroxyapatite hybrid nanofiber scaffold for bone regeneration |
| CN101703795A (en) * | 2009-11-11 | 2010-05-12 | 复旦大学附属中山医院 | Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material |
| CN101773684A (en) * | 2010-03-19 | 2010-07-14 | 西南大学 | Preparation method of fibroin/hydroxyapatite porous scaffold |
Non-Patent Citations (3)
| Title |
|---|
| LI WANG ETAL.: ""Effects of alkali pretreatment of silk fibroin on microstructure and properties of hydroxyapatite-silk fibroin nanocomposite"", 《JOURNAL OF MATERIALS SCIENCE： MATERIALS IN MEDICINE》, vol. 15, no. 3, 31 March 2004 (2004-03-31), pages 261 - 265 * |
| LI WANG ETAL.: ""Microstructure and gelation behavior of hydroxyapatite-based nanocomposite sol containing chemically modified silk fibroin"", 《COLLOIDS AND SURFACES A： PHYSICOCHEMICAL AND ENGINEERING ASPECTS》, vol. 254, no. 13, 8 January 2005 (2005-01-08), pages 159 - 164 * |
| 刘琳: ""类骨多孔羟基磷灰石/丝素蛋白复合支架的制备及生物相容性研究"", 《浙江理工大学硕士学位论文》, 31 December 2008 (2008-12-31), pages 18 - 19 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105688283A (en) * | 2016-02-01 | 2016-06-22 | 苏州大学 | Modified silk fibroin-calcium phosphate composite bone cement and preparation method thereof |
| CN105688283B (en) * | 2016-02-01 | 2019-01-25 | 苏州大学 | A kind of modified fibroin albumen-compound bone cement of calcium phosphate and preparation method thereof |
| CN108404208A (en) * | 2018-05-25 | 2018-08-17 | 姜香 | A kind of preparation method of wear-resistant type artificial joint material |
| CN108914241A (en) * | 2018-07-20 | 2018-11-30 | 广西科技大学 | A kind of preparation method of quickly controllable silk fibroin staple fiber |
| CN108914241B (en) * | 2018-07-20 | 2020-08-18 | 广西科技大学 | Preparation method of fast controllable cocoon silk protein short fiber |
| CN110180029A (en) * | 2019-05-16 | 2019-08-30 | 暨南大学 | A kind of preparation method and application with induced osteogenesis differentiation and the degradation material of bone regeneration function |
| CN110180029B (en) * | 2019-05-16 | 2022-02-25 | 暨南大学 | Preparation method and application of degradable material with osteogenic differentiation and bone regeneration induction functions |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL273159B1 (en) | Culture medium composition, and method for culturing cell or tissue using said composition | |
| CN103143059B (en) | Nano-composite bone defect repair support with multistage pore diameter structure | |
| CN101474429B (en) | Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method | |
| CN103418027A (en) | Preparation method of composite porous scaffold material | |
| CN102430155B (en) | Cellular silk fibroin porous scaffold, and preparation method thereof | |
| CN101897994A (en) | Biological composite scaffold for repairing bone defect and preparation method thereof | |
| CN103690991A (en) | Preparation method of cartilage repair scaffold material | |
| CN105816914A (en) | Method for preparing fibroin hydroxyapatite composite material through electrochemical deposition | |
| CN103990180A (en) | Preparation method and application of deproteinated decalcification bone matrix implantable microcarrier | |
| CN101327341A (en) | Method for preparing nano-scale fibroin-hydroxylapatite mineralized complex fiber | |
| CN105797217A (en) | Porous micro-sphere bone repair material and preparing method thereof | |
| CN104289197B (en) | A kind of amination strengthens modified straw based composites and preparation method thereof | |
| CN103691002A (en) | Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof | |
| CN102580162A (en) | Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent | |
| CN102973980A (en) | Inorganic/organic diphase nano composite bone tissue engineering scaffold and preparation method thereof | |
| CN103272281B (en) | Carbon fiber-based hydroxyapatite/chitosan composite porous scaffold material and preparation method thereof | |
| CN102580145B (en) | Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent | |
| CN113846045A (en) | Preparation method of fish scale tissue scaffold for cell culture | |
| CN102106765B (en) | Three-dimensional bone-like structure artificial bone with high bioactivity and high strength and preparation method thereof | |
| CN106512099B (en) | Chitosan-based biotic bone tissue engineering stent and preparation method thereof | |
| RU2021126356A (en) | A method of creating tissue-engineered constructions by the method of bioprinting with bio-ink for the regeneration of tissues and organs in the conditions of an organism | |
| CN103554543B (en) | A kind of preparation method of konjac glucomannan fiber scaffold material | |
| CN103114071B (en) | Method for rapidly and concentratedly preparing porphyra haitanensis protoplast | |
| CN106693046A (en) | Gel type bone repair material and preparation method thereof | |
| CN102585277A (en) | Process for preparing keratin porous membrane by refrigerating |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131204 |