CN103418018A - Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof - Google Patents
Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103418018A CN103418018A CN2013103204203A CN201310320420A CN103418018A CN 103418018 A CN103418018 A CN 103418018A CN 2013103204203 A CN2013103204203 A CN 2013103204203A CN 201310320420 A CN201310320420 A CN 201310320420A CN 103418018 A CN103418018 A CN 103418018A
- Authority
- CN
- China
- Prior art keywords
- tea fungus
- synthetic
- bacterial cellulose
- dressing
- red tea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a tea fungus synthesized bacterial cellulose pressure sore dressing as well as a preparation method and the application thereof. The tea fungus synthesized bacterial cellulose pressure sore dressing is a tea fungus synthesized bacterial cellulose membrane, and the cellulose component is mainly formed by secretion and synthesis of tea fungus strains in the fermentation culturing process in a sugar tea culture medium containing carboxymethyl chitosan; the water absorption rate of the dressing is 89-94.3%, the porosity is 85-91.2%, and the water vapor transmission rate is between 1,383.5 g.m<-2>.d<-1> and 1,486.5 g.m<-2>.d<-1>. According to the invention, since the nano bacterial cellulose pressure sore dressing is prepared with a sugar tea fermentation method by adopting the tea fungus, the method for synthesis of bacterial cellulose has the advantages of low cost, low environment requirement for synthesis sites and simple technical requirements; the nano bacterial cellulose material has favorable biosecurity and histocompatibility, which cannot cause stimulation and sensitization to a wound surface, and is conducive to the promotion of wound healing.
Description
Technical field
The present invention relates to synthetic Bacterial cellulose pressure ulcer dressing of a kind of red tea fungus and its production and use.
Background technology
Pressure ulcer is skin, muscle and the damage of hypodermic limitation that skin or subcutaneous tissue cause due to pressure, shearing force or frictional force, often occurs in bone knuckle place.The incidence rate of pressure ulcer is high and recover slowly, if treatment and nurse improperly can cause the necrosis of muscle and skeleton even to cause systemic infection, when serious, can threaten patient's life.Mainly utilize clinically various dressing to cover and protect wound surface and provide and be beneficial to the microenvironment that wound surface heals as early as possible and recovers.Although it is a variety of that current pressure ulcer dressing type has, and its corresponding limitation is arranged.As common gauze dressing, although having, it absorbs sepage, protection wound surface, cheap characteristics, be easy to and wound exudate forms dry crusts pain and the easy shortcoming such as pollution while changing dressings.Therefore develop a kind of good water absorption, be difficult for and wound exudate formation dry crusts, wound healing, pressure ulcer dressing with low cost, for the relevant disease patient provide a kind of selection be current market in the urgent need to.
Red tea fungus is as a kind of acidic beverages of folk tradition, and that its working condition requires is low, production technology simply reaches with low costly, and long fermenting and producing and applicating history are at home and abroad arranged.Strain for the fermentation culture red tea fungus is mainly acetic acid bacteria and yeast or contains part lactobacillus [being mainly Lactobacillus bulgaricus (Lactobacterium bulagricum)] simultaneously.Mainly comprise that gluconate pyracetobacillus (also claims acetobacter xylinum in the at present known acetic acid Pseudomonas be separated to from various red tea funguss, Acetobacter xylinum), intend acetobacter xylinum (Acetobacter xylinoides), Bacterium gluconicum (Bacterium gluconicum), acetobacter aceti (Acetobacter aceti) and Acetobacter pasteurianus (Acetobacter pasteurianus) etc., wherein acetobacter xylinum most importantly; Mainly comprise schizosaccharomyces pombe (Schizosaccharomyces pombe) in Saccharomycodes, rood class yeast (Saccharomycodes ludwigii), saccharomyces cerevisiae (Saccharomyces cerevisiae), etc. multiple [C. Dufresne, E. Farnworth. Tea, Kombucha, and health:a review, 2000,33 (6), 409-421].In the process of red tea fungus fermentation, have the translucent membranaceous floating thing of one deck and be positioned at gas-liquid interface.This membranaceous material is that acetic acid bacteria is secreted synthetic Bacterial cellulose composition, according to relevant research, show, its architectural feature be typical I α fiber type element architectural feature [
Zhu C, et al., Kombucha-synthesized bacterial cellulose:Preparation, characterization and biocompatibility evaluation. Journal of Biomedical Materials Research Part A, 2013, DOI:10.1002/jbm.a.34796.].This Bacterial cellulose has unique physicochemical characteristics (as high-crystallinity, high retentiveness, superfine nano network of fibers, high-tensile etc.) and the good interior biocompatibility of body.Have no at present the Bacterial cellulose synthetic by the red tea fungus relevant report for medical pressure ulcer dressing.
Summary of the invention
The object of the present invention is to provide synthetic Bacterial cellulose pressure ulcer dressing of a kind of red tea fungus easy to prepare, that be beneficial to wound healing and its production and use.
Technical solution of the present invention is:
The Bacterial cellulose pressure ulcer dressing that a kind of red tea fungus is synthetic is characterized in that: be the synthetic bacteria cellulose film of red tea fungus, cellulose components is mainly secreted synthetic by the red tea fungus bacterial strain in the glucose-tea broth culture medium that contains carboxymethyl chitosan in the fermentation culture process; The water absorption rate of dressing is 89 ~ 94.3%, and porosity is 85 ~ 91.2%, and moisture-vapor transmission is 1435 ± 51.5 g.m
-2.d
-1.
The preparation method of the Bacterial cellulose pressure ulcer dressing that a kind of red tea fungus is synthetic, is characterized in that: comprise the following steps:
(1) according to weight percent meter, will add in the beaker containing the 500mL distilled water containing 0.5 ~ 2% black tea or green tea, 10 ~ 20% sucrose or glucose, boil after 5 ~ 15 minutes and be cooled to room temperature, obtain the solution I;
(2) after solution I filtration above-mentioned steps (1) made, removal tea leaf residual, add respectively the yeast, ethanol, carboxymethyl chitosan and the peptone that are respectively 0.5 ~ 1wt%, 0.5 ~ 1%v/v, 0.5 ~ 1wt% and 0.5 ~ 1wt% with respect to whole content percentage by weight or percent by volume, stirring and dissolving, obtain the solution II;
(3) the solution II in above-mentioned steps (2) is adjusted to respectively to 1L and 4.5 ~ 5.5 by cumulative volume and pH value, pasteurization sterilizing 20 ~ 30 minutes by distilled water, 1 ~ 5% acetum;
(4) freshly prepared basal medium in above-mentioned steps (3) is poured into to one and covered, in the clean container of ventilation, add the red tea fungus maternal plant containing 3 ~ 5 layers of clean gauze, under 30 ° of C constant temperature, clean environment, standing cultivation is 7 ~ 10 days;
(5) during the incubation time in reaching above-mentioned steps (4), to in culture medium, carefully take out by translucent, the gelatinous membrane of solution-air interface, this film is the synthetic bacteria cellulose film of red tea fungus, the bacteria cellulose film that red tea fungus is synthetic is with distilled water rinsing 3 times, each 10 ~ 15 minutes, except residual culture medium and the impurity component on striping;
(6) the synthetic bacteria cellulose film of gel red tea fungus in above-mentioned steps (5) is soaked in the NaOH solution that molar concentration is 0.05 ~ 0.1M, boils 15 ~ 20 minutes, further remove thalline residual on film and other impurity component;
(7) the synthetic bacteria cellulose film of the red tea fungus that will process through above-mentioned steps (6) is with two steaming rinsings, identical with distilled water used with the pH value of measuring film till;
(8) the synthetic bacteria cellulose film lyophilization of red tea fungus that will process through step (7), cut into needed specification, and packing is by applying after the Co-60 irradiation sterilization.
The application of Bacterial cellulose pressure ulcer dressing in the dressing of preparation treatment pressure ulcer that a kind of red tea fungus is synthetic.
The present invention prepares nanometer bacteria cellulose pressure ulcer dressing with red tea fungus by the glucose-tea broth fermentation method, the low simple advantage of specification requirement that reaches of environmental requirement with low cost, that synthesize place that the mode of this kind of synthetic Bacterial cellulose has; Because this nanofiber cellulosic material has good biological safety and histocompatibility, can not cause stimulation and sensitization to wound surface, be conducive to promote wound healing; This nanofiber have good retentiveness, and discord organizes sepage to form dry crusts, when changing, very easily takes off, and does not cause the misery of patient when change dressings more.Use carboxymethyl chitosan in culture medium, increased the nanofiber gel film in performance characteristics such as dried secondary water absorption and breathability.
The accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 is the scanning electron microscope (SEM) photograph of the synthetic Bacterial cellulose pressure ulcer dressing structure of red tea fungus of the present invention.
The specific embodiment
Embodiment 1:
The preparation of KBC: adopt the preparation of tea fermentation method.
(1) according to weight percent meter, in the culture medium system that to suppose at cumulative volume be 1L, will add in the beaker containing the 500mL distilled water containing 0.5 ~ 2% black tea, 10 ~ 20% sucrose, boil after 5 ~ 15 minutes and be cooled to room temperature;
(2) after the solution in above-mentioned steps (1) fully being filtered, removing tea leaf residual, add respectively with respect to whole content percentage by weight (or percent by volume, v/v) be respectively 0.5 ~ 1%, 0.5 ~ 1%(v/v), 0.5 ~ 1% and 0.5 ~ 1% yeast, ethanol, carboxymethyl chitosan and peptone, fully stirring and dissolving;
(3) solution in above-mentioned steps (2) is adjusted to respectively to 1L and 4.5 ~ 5.5 by cumulative volume and pH value, pasteurization sterilizing 20 ~ 30 minutes by distilled water, 1 ~ 5% acetum;
(4) freshly prepared basal medium in above-mentioned steps (3) is poured into one with 3 ~ 5 layers of clean gauze cover, the clean tray of ventilation (or other suitable vessel as flask etc.), add the red tea fungus maternal plant, under 30 ° of C constant temperature, clean environment, standing cultivation is 7 ~ 10 days;
(5) during the incubation time in reaching above-mentioned steps (4), to in culture medium, carefully take out by translucent, the gelatinous membrane of solution-air interface, this film is the KBC film, by the KBC film with the abundant rinsing of distilled water 3 times, each 10 ~ 15 minutes, except residual culture medium and the impurity component on striping;
The purification of KBC
(6) the gel KBC film in above-mentioned steps (5) is soaked in the NaOH solution that molar concentration is 0.05 ~ 0.1M, boils 15 ~ 20 minutes, further remove thalline residual on film and other impurity component;
(7) by the KBC film in above-mentioned steps (6) with the abundant rinsing of two steamings, identical with distilled water used with the pH value of measuring film till.
(8) by the KBC film lyophilization of step (7), cut into needed specification, packing is by applying after the Co-60 irradiation sterilization.
The Bacterial cellulose pressure ulcer dressing that the red tea fungus obtained is synthetic, cellulose components is mainly secreted synthetic by the red tea fungus bacterial strain in the glucose-tea broth culture medium that contains carboxymethyl chitosan in the fermentation culture process; The water absorption rate of dressing is 89 ~ 94.3%, and porosity is 85 ~ 91.2%, and moisture-vapor transmission is 1435 ± 51.5 g.m
-2.d
-1.
Embodiment 2:
0.5 ~ 2% black tea in step (1), 10 ~ 20% sucrose replace with respectively 0.5 ~ 2% green tea, 10 ~ 20% glucose, and all the other are with embodiment 1.
Embodiment 3:
0.5 ~ 2% black tea in step (1), 10 ~ 20% sucrose replace with respectively sucrose, the glucose mixing additive that black tea, green tea mixing additive and the total content of total content 0.5 ~ 2% are 10 ~ 20%, and all the other are with embodiment 1.
Claims (3)
1. the Bacterial cellulose pressure ulcer dressing that red tea fungus is synthetic, it is characterized in that: be the synthetic bacteria cellulose film of red tea fungus, cellulose components is mainly secreted synthetic by the red tea fungus bacterial strain in the glucose-tea broth culture medium that contains carboxymethyl chitosan in the fermentation culture process; The water absorption rate of dressing is 89 ~ 94.3%, and porosity is 85 ~ 91.2%, and moisture-vapor transmission is 1435 ± 51.5 g.m
-2.d
-1.
2. the preparation method of the synthetic Bacterial cellulose pressure ulcer dressing of a red tea fungus, is characterized in that: comprise the following steps:
(1) according to weight percent meter, will add in the beaker containing the 500mL distilled water containing 0.5 ~ 2% black tea or green tea, 10 ~ 20% sucrose or glucose, boil after 5 ~ 15 minutes and be cooled to room temperature, obtain the solution I;
(2) after solution I filtration above-mentioned steps (1) made, removal tea leaf residual, add respectively the yeast, ethanol, carboxymethyl chitosan and the peptone that are respectively 0.5 ~ 1wt%, 0.5 ~ 1%v/v, 0.5 ~ 1wt% and 0.5 ~ 1wt% with respect to whole content percentage by weight or percent by volume, stirring and dissolving, obtain the solution II;
(3) the solution II in above-mentioned steps (2) is adjusted to respectively to 1L and 4.5 ~ 5.5 by cumulative volume and pH value, pasteurization sterilizing 20 ~ 30 minutes by distilled water, 1 ~ 5% acetum;
(4) freshly prepared basal medium in above-mentioned steps (3) is poured into to one and covered, in the clean container of ventilation, add the red tea fungus maternal plant containing 3 ~ 5 layers of clean gauze, under 30 ° of C constant temperature, clean environment, standing cultivation is 7 ~ 10 days;
(5) during the incubation time in reaching above-mentioned steps (4), to in culture medium, carefully take out by translucent, the gelatinous membrane of solution-air interface, this film is the synthetic bacteria cellulose film of red tea fungus, the bacteria cellulose film that red tea fungus is synthetic is with distilled water rinsing 3 times, each 10 ~ 15 minutes, except residual culture medium and the impurity component on striping;
(6) the synthetic bacteria cellulose film of gel red tea fungus in above-mentioned steps (5) is soaked in the NaOH solution that molar concentration is 0.05 ~ 0.1M, boils 15 ~ 20 minutes, further remove thalline residual on film and other impurity component;
(7) the synthetic bacteria cellulose film of the red tea fungus that will process through above-mentioned steps (6) is with two steaming rinsings, identical with distilled water used with the pH value of measuring film till;
(8) the synthetic bacteria cellulose film lyophilization of red tea fungus that will process through step (7), cut into needed specification, and packing is by applying after the Co-60 irradiation sterilization.
3. the application of Bacterial cellulose pressure ulcer dressing in the dressing of preparation treatment pressure ulcer that red tea fungus is synthetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103204203A CN103418018A (en) | 2013-07-29 | 2013-07-29 | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103204203A CN103418018A (en) | 2013-07-29 | 2013-07-29 | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103418018A true CN103418018A (en) | 2013-12-04 |
Family
ID=49643753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103204203A Pending CN103418018A (en) | 2013-07-29 | 2013-07-29 | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103418018A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966700A (en) * | 2014-05-27 | 2014-08-06 | 哈尔滨工业大学 | Method for preparing carbon nanofiber aerogel oil absorption material from bacterial cellulose |
| CN104630311A (en) * | 2015-02-09 | 2015-05-20 | 江苏联海生物科技有限公司 | Method for synchronously producing straw nano-cellulose and bacterial cellulose by using sweet sorghum |
| CN105624041A (en) * | 2016-01-22 | 2016-06-01 | 李支群 | Culture medium of tea fungus bacterial liquid, preparation method of tea fungus bacterial liquid and application of tea fungus bacterial liquid |
| WO2017132671A1 (en) * | 2016-01-29 | 2017-08-03 | Genadyne Biotechnologies, Inc. | System and method for treating a wound |
| CN107881116A (en) * | 2017-09-25 | 2018-04-06 | 贵州棒棒食用菌产业有限公司 | A kind of method that pork tripe bacteria liquid strain is prepared using glucose-tea broth as primary raw material |
| CN108888799A (en) * | 2018-06-25 | 2018-11-27 | 东莞市联洲知识产权运营管理有限公司 | A kind of enhanced compound cellulose medical dressing and preparation method thereof with disinsectization performance |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090209897A1 (en) * | 2008-02-20 | 2009-08-20 | Lotec, Inc. Dba Vesta Sciences, Inc. | Photoactivated Antimicrobial Wound Dressing and Method Relating Thereto |
| CN101700408A (en) * | 2009-11-05 | 2010-05-05 | 绍兴振德医用敷料有限公司 | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof |
| TW201121588A (en) * | 2009-12-23 | 2011-07-01 | Taiwan Textile Res Inst | Composition film, the process for the production thereof and use thereof |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
| CN103007335A (en) * | 2012-12-28 | 2013-04-03 | 海南大学 | Method for preparing chitosan oligosaccharide/bacterial cellulose sponge |
-
2013
- 2013-07-29 CN CN2013103204203A patent/CN103418018A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090209897A1 (en) * | 2008-02-20 | 2009-08-20 | Lotec, Inc. Dba Vesta Sciences, Inc. | Photoactivated Antimicrobial Wound Dressing and Method Relating Thereto |
| CN101700408A (en) * | 2009-11-05 | 2010-05-05 | 绍兴振德医用敷料有限公司 | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof |
| TW201121588A (en) * | 2009-12-23 | 2011-07-01 | Taiwan Textile Res Inst | Composition film, the process for the production thereof and use thereof |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
| CN103007335A (en) * | 2012-12-28 | 2013-04-03 | 海南大学 | Method for preparing chitosan oligosaccharide/bacterial cellulose sponge |
Non-Patent Citations (1)
| Title |
|---|
| 唐水佳: "红茶菌制备细菌纤维素的研究", 《纤维素科学与技术》, vol. 20, no. 2, 30 June 2012 (2012-06-30), pages 40 - 45 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966700A (en) * | 2014-05-27 | 2014-08-06 | 哈尔滨工业大学 | Method for preparing carbon nanofiber aerogel oil absorption material from bacterial cellulose |
| CN103966700B (en) * | 2014-05-27 | 2016-03-30 | 哈尔滨工业大学 | Bacteria cellulose is utilized to prepare the method for carbon nano-fiber aeroge oil absorption material |
| CN104630311A (en) * | 2015-02-09 | 2015-05-20 | 江苏联海生物科技有限公司 | Method for synchronously producing straw nano-cellulose and bacterial cellulose by using sweet sorghum |
| CN105624041A (en) * | 2016-01-22 | 2016-06-01 | 李支群 | Culture medium of tea fungus bacterial liquid, preparation method of tea fungus bacterial liquid and application of tea fungus bacterial liquid |
| WO2017132671A1 (en) * | 2016-01-29 | 2017-08-03 | Genadyne Biotechnologies, Inc. | System and method for treating a wound |
| US11491256B2 (en) | 2016-01-29 | 2022-11-08 | Genadyne Biotechnologies, Inc. | System and method for treating a wound |
| CN107881116A (en) * | 2017-09-25 | 2018-04-06 | 贵州棒棒食用菌产业有限公司 | A kind of method that pork tripe bacteria liquid strain is prepared using glucose-tea broth as primary raw material |
| CN108888799A (en) * | 2018-06-25 | 2018-11-27 | 东莞市联洲知识产权运营管理有限公司 | A kind of enhanced compound cellulose medical dressing and preparation method thereof with disinsectization performance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103418018A (en) | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof | |
| CN102727926B (en) | The preparation method of the composite wound dressing of a kind of polysaccharide and nanometer bacteria cellulose | |
| CN102552965B (en) | Method for preparing nano-cellulose antibacterial composite material through on-line culture | |
| CN101921700B (en) | Device and method for preparing hollow heteromorphic bacteria cellulose material | |
| CN101905031B (en) | Method for preparing flamazine/bacterial cellulose composite wound dressing | |
| CN103340722B (en) | Sanitary towel good in water-absorbing performance | |
| CN101914434B (en) | Device and method for dynamically preparing heterocavity bacterium cellulose materials | |
| CN103550817B (en) | A kind of Bacterial cellulose/shitosan composite sponge dressing and preparation method thereof | |
| CN106215232A (en) | A kind of wound antibacterial promoting healing dressing and preparation method thereof | |
| CN103480027B (en) | Preparation method of bacterial cellulose composite chitosan fiber moist dressing | |
| KR101702590B1 (en) | Mask Pack and Method for Fabricating Thereof | |
| CN103480042B (en) | Artificial dura mater spinalis, and preparation method and use method thereof | |
| WO2017126929A1 (en) | Method for preparing dry bio cellulose and dry bio cellulose prepared thereby | |
| CN104587519B (en) | A kind of dressing paste containing Chinese medicine | |
| CN103083136B (en) | Bacterial cellulose liquid absorbing material of sandwich structure and preparation method of bacterial cellulose liquid absorbing material | |
| CN104436291B (en) | A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer | |
| CN113101405A (en) | Method for preparing protective wound dressing | |
| CN201809342U (en) | Device for dynamically preparing profiled cavity bacterial cellulose material | |
| CN102274540A (en) | Compound sponge material and preparation method thereof | |
| CN104130424B (en) | A kind of preparation method of hyaluronic acid/bacteria cellulose composite hydrogel | |
| CN110403858A (en) | Bacteria cellulose/Pu'er tea composite functional material and preparation method thereof | |
| CN103349784B (en) | Injectable microsphere particle hydrogel medicine carrying body and preparation method thereof and application | |
| CN204158771U (en) | A kind of Bacterial cellulose/chitosan composite sponge dressing | |
| KR101788791B1 (en) | Manufacturing method of bio cellulous sheet having curcumin | |
| CN103394113B (en) | Adhesive bandage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131204 |