CN103416592A - Industrial production method of rice protein hydrolysate for feed - Google Patents
Industrial production method of rice protein hydrolysate for feed Download PDFInfo
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Abstract
The invention discloses an industrial production method of rice protein hydrolysate for feed, and belongs to the technical field of feed additive production. The industrial production method of the rice protein hydrolysate for the feed comprises the steps that (1) the method of synergism of various enzymes and multi-step joint processing is adopted, the solid/liquid weight ratio between rice protein powder and water is 1:2-10, and alkaline proteinase is added to carry out dissolution; (2) a complex enzyme preparation which is formed by the alkaline proteinase, neutral proteinase, papain and flavourzyme in a compound mode is added to carry out hydrolysis, and the working procedures such as enzyme deactivation, filtration, decolorization and spray drying are carried out to obtain a rice protein hydrolysate product for the feed. Compared with the traditional method, the hydrolysis conditions are moderate, hygiene and safety are achieved, the situation that original amino-acid composition nutrients are not damaged is ensured, the hydrolysate is low in salinity and mellow in taste, and the yield is high and can reach 98%. According to application tests of adding the rice protein hydrolysate to feed such as feed of suckling pigs, feed of chicks, feed of calves and feed of aquaculture, the rice protein hydrolysate for the feed has the advantages of increasing the daily food-intake amount, reducing the diarrhea rate, improving immunity, reducing weaning stress, and improving growth performance.
Description
Technical field
The industrialized preparing process of method of rice protein hydrolysate for a kind of feed, relate to comprehensive exploitation application and the technical field of feed additive production of vegetable protein.
Background technology
Nearly 200,000,000,000 kilograms of China's paddy annual production, account for 35% left and right of Gross World Product, occupies first place in the world.Wherein the output of early rice accounts for 1/4 left and right of paddy total output.Current, national grain supply and demand is in a basic balance, and the rice total amount is abundant.Poor rice morning, the old rice of quality is cold-shouldered, and the stock is full, can not sell, and causes the serious waste of grain resource and huge economic loss.The byproduct of exploitation high added value, strengthen raw material is fully utilized, and is the effective way that reduces loss.
Rice protein accounts for 8% left and right of rice gross weight, have high nutrition, hypoallergenic, local flavor gentle, can not cause the peculiar property such as ventosity.Its application is more and more extensive, as be used as, produces rice protein powder, hydrolysed rice albumen, rice modified protein, high added value peptide, biologically active peptide, resistance protein; Can be used as the cub feed addictive, improve the absorption of cub to nutrition, improve premunition.
Method of rice protein hydrolysate as a kind of high-quality without antigen vegetable protein source, the countries such as Europe be widely used in newborn baby pig feedstuff, high-grade aquatic products material, calf for milk powder and feed for pet in.Be characterized in having very high glutamine content (up to 30%), documents and materials show: stress or limit and raise (during weaned piglet) under condition, glutamate is main restrictive factor, the weanling pig artificial synthetic glutamate of feeding, can improve small intestine alimentary canal shape (intestinal villi structure), improve immune response.Although domestic, have producer using this product, this product there is no suitability for industrialized production at home.
Method of rice protein hydrolysate is to take natural vegetable protein through modification, to form as raw material, it is the green safety plant protein products, can overcome the bio-safety problem of plasma protein homology, can avoid again soybean protein to meet with genetically modified trade barrier, not have the restriction in application.Latest research shows, with hydrolysis, wait the biochemical treatment Gluten to make the protein hydrolysate feed piglet is had to very high digestibility, without ANFs, can promote the growth of intestine of young pigs fine hair, obtain higher feed efficiency, the spray-drying plasma protein (2.5%～8%) of alternative costliness.At present, the research of domestic relevant hydrolysis defatted soybean, cotton dregs is more, less about the research report of enzyme modification rice protein.(the Faoming properties of enzymatially hydrolysed wheat gletun such as Drago, Cereal Sci, 2000,32:114-135) adopt the heat treated Gluten of fungal proteinase hydrolyze to find, Gluten solubility increases and increases along with degree of hydrolysis (DH), but foaming characteristic when exceeding certain DH scope by destroyed.Qi Junru etc. (Zhengzhou Engineering College's journal, 04 phase of calendar year 2001,48-51 page) adopt the neutral proteinase hydrolysis rice protein, and have studied the controlled hydrolysis rice protein, and show: controlled hydrolysis has improved the functional characteristic of rice protein greatly.(the grain and feed industries such as Zhao Dongyan, 08 phase in 2003,39-41 pages) improve the functional of rice protein with alkali protease (Alcalase), studied the impact on the rice protein emulsibility of rice protein concentration, enzyme concentration, pH value, heat time heating time and temperature, and, on this basis by orthogonal experiment, explore the optimum condition that alkali protease improves rice protein function.Recording the front rice protein solubility of modification is 7.67%, and under the optimum condition of modification, its solubility is 20.76%, and as can be seen here, the solubility of rice protein is greatly improved after the Alcalase hydrolysis; When the rice protein modification obtains the optimum emulsification effect, its degree of hydrolysis is 3.2%.(the food and fermentation industries such as Huang Jihong, 2004,30 (1): 92-94) to adopting the enzymatic hydrolysis rice protein to produce biological activity protein, carried out pilot study, think that enzymatic hydrolysis is the effective way that improves the rice protein food function, can be by protein-modified to desired functional characteristic.
From above-mentioned report, the correlative study of enzymatic hydrolysis rice protein at present all rests on laboratory stage, does not carry out further scale-up research, also could not realize suitability for industrialized production, and enzyme preparation adopts single composition more.Carrying out the feeding method of rice protein hydrolysate product of industrial-scale production by enzyme modification have not been reported.It is the ideal substitute of animal protein that rice protein generates the rice functional protein through enzyme modification, aspect animal-breeding, has broad application prospects and huge market potential.
Summary of the invention
For meeting the need of market, the object of the invention is to provide a kind of new method that adopts the feeding rice protein of composite protease hydrolysis, and realizes suitability for industrialized production.
For realizing the object of the invention, technical scheme is as follows:
According to the characteristic of rice Gluten and the characteristics of animal nutrition, utilize chemistry and enzyme process to carry out modification to rice protein, start with and inquired into the impact of each factor on the rice protein enzymatic hydrolysis from five factors (solid-to-liquid ratio, pH value, enzyme concentration, temperature and time) of restriction rice protein hydrolysis, obtain process conditions on the orthogonal experiment basis, through pilot scale and suitability for industrialized production test, obtain feeding method of rice protein hydrolysate product.Product is rich in glutamine, and the form mainly with little peptide exists greatly, and molecular weight is less than 2000 daltonian little peptides and accounts for more than 60%, wherein is less than 1000 daltonian little peptides and accounts for more than 50%.
The industrialized preparing process of this feeding method of rice protein hydrolysate product specifically prepares by following steps:
(1) dissolve: take rice protein powder, mix with 10-50 ℃ of warm water in reactor, rice protein powder and water solid-liquid weight ratio 1 ︰ 2-10; Add alkali protease to be dissolved, its addition is the 20-50U/g rice protein powder;
(2) hydrolysis: step (1) gained solution is regulated pH value 5.0-10.0, adds complex enzyme formulation, and making its mass percentage concentration in solution is 0.2%-1.4%, at temperature 30-70 ℃, and hydrolysis 1-13 h;
Complex enzyme formulation is that alkali protease, neutral proteinase, papain, flavor protease are with composite the forming of 1 ︰ 1-2 ︰ 0.1-0.5 ︰ 0.1-0.2 weight ratio;
(3) then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment: the decolorizer used that decolours is active carbon, and the 1%-4% that its addition is rice protein powder weight processes 10-130min at 50-90 ℃; EAT 130-160 ℃ for Bian, leaving air temp 60-85 ℃, it is thousand dry that homogenization pressure 10-30MPa is sprayed, and obtains the feeding method of rice protein hydrolysate of powdery.
Regulate pH value acid or alkali used and be respectively hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, NaOH or potassium hydroxide.The decolorizer used that decolours is active carbon, the 1%-4% that its addition is rice protein powder weight.
Optimum process condition is: in dissolving step, the weight ratio of rice protein powder and water is 1 ︰ 5; Add complex enzyme formulation in hydrolysing step, making its mass percentage concentration in solution is 0.6%, water transfer solution pH value 6.5,40 ℃ of hydrolysis temperatures, hydrolysis time 4h.
Beneficial effect of the present invention:
The method hydrolysed rice albumen of plurality of enzymes synergy, multi-step Combined Treatment for Bian of the present invention.The product of this explained hereafter is to take the deep processing conversion product that agricultural byproducts carry out as raw material, and added value is large, produces environment-protecting clean in production process without the three wastes.The configuration of this apparatus for production line is advanced, production technology is scientific and reasonable, and integrated production capacity is strong, and output, yield are high, reach 98%.Produce and stablize, product nitrogen solubilized Exponential Stability is more than 90%, and emulsibility and emulsion stability are all over 80%, and property indices is significantly improved than Gluten and improves.With conventional method, compare: biological enzyme is due to the hydrolysising condition gentleness, and safe and sanitary, kept original amino acid to form nutrition and do not lost, and the hydrolysate salinity is low, good palatability; And traditional diamond-making technique adopts acid and alkali hydrolysis usually, easily cause the nutritional labeling loss, be hydrolyzed without specificity side reaction many (producing venomous injurant trichloropropanols etc.), the problems such as safe and sanitary.Experimental result is as table 1 and table 2.
The method of rice protein hydrolysate that table 1 the present invention produces contrasts with rice protein physico-chemical analysis result.
Method of rice protein hydrolysate and rice protein partial peptide molecular weight distribution result that table 2 the present invention produces.
Annotate: above data are that the sample soluble part detects gained molecular weight result.
Innovative point of the present invention is: in conjunction with characteristic and the animal nutrition characteristics of rice protein, by plurality of enzymes act synergistically, the method for multi-step Combined Treatment is hydrolyzed the rice protein test, has developed novel method of rice protein hydrolysate product.Through continuous pilot plant test and large-scale production checking, can realize producing per year 10000 tons of production capacity.Product adds application after DIFFERENT FEED production unit preparation in the feeds such as sucking pig, chick, calf cultivation, has advantage and the positive effects such as daily ingestion amount increases (good palatability), reduces diarrhea rate, strengthens immunity, slows down ablactation stress, reduces feedstuff-meat ratio, raising growth performance.
The feeding method of rice protein hydrolysate product that the present invention produces is compared and had following features with Gluten: (1) is after modification, protein by water insoluble become solvable, improved digestive utilization ratio, digestibility reaches more than 96%, can directly drink with milk powder or other allied substances compatibility; (2) high molecular weight protein in protein is hydrolyzed to small molecular protein, and the form mainly with little peptide exists greatly, and molecular weight is less than 2000 daltonian little peptides and accounts for more than 60%, wherein is less than 1000 daltonian little peptides and accounts for more than 50%, is more conducive to young animal and absorbs; (3) amino acid forms complete, wherein glutamine content is up to more than 28%, and exist mainly with the oligopeptides form, in enteron aisle, be easy to absorb, glutamine energy repairing damage intestinal mucosa also maintains the complete of enteron aisle 26S Proteasome Structure and Function, synthesizing of the hyperplasia of Promote immunity cell and immunologic active material, the enhanced machine body immunity function, improve resistance against diseases; (4) there is special local flavor and good food calling effect; (5) protein content is high, and emulsibility and emulsion stability are good, is convenient to use with other material collocation, plays synergistic function.
The feeding method of rice protein hydrolysate that the present invention produces is compared the glutamine with following advantage: a, rich content with SDPP significant period at the special physiological of weaned piglet, to the maintenance of sucking pig Intestinal Morphology, is that SDPP is not available; B, as a kind of functional plants protein sources, do not have any biological safety problem; C, otherness and source of goods instability problem that SDPP exists have been overcome.The feeding method of rice protein hydrolysate raw material abundance that the present invention produces, stable processing technique, can guarantee uniformity and the stability of product quality.D, overcome blood plasma and contain higher animal tallow, easily oxidation deterioration affects the palatability problem of feed.
The specific embodiment
The present invention is further illustrated for the following examples.
Embodiment 1
Take rice protein powder 1000kg, mix with 40 ℃ of warm water of 6000kg in reactor, add alkali protease to be dissolved, its addition is the 20U/g rice protein powder; Then regulate pH value 7.5 with sodium hydroxide solution, add complex enzyme formulation, making its mass percentage concentration in solution is 0.3%, at 40 ℃ of hydrolysis 3h of temperature, complex enzyme formulation used is: alkali protease, neutral proteinase, papain, flavor protease form so that the 1:1:0.2:0.2 weight portion is composite.Then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment, filter decolouring and adopt active carbon, 2 % that its addition is rice protein powder weight, process 10-130min 50-90 ℃ of decolouring.Adopt 160 ℃ of EATs, 85 ℃ of leaving air temps, homogenization pressure 30MPa carries out spray-drying, makes the feeding method of rice protein hydrolysate of product.
Embodiment 2
Take rice protein powder 1000kg, mix with 35 ℃ of warm water of 7000kg in reactor, add alkali protease to be dissolved, its addition is the 40U/g rice protein powder; Then regulate pH value 7.0 with potassium hydroxide solution, add complex enzyme formulation, making its mass percentage concentration in solution is 0.2%, at temperature 45 C hydrolysis 6h, complex enzyme formulation used is: alkali protease, neutral proteinase, papain, flavor protease are with composite the forming of 1 ︰ 1.5 ︰ 0.3 ︰ 0.1 weight portion.Then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment, filter decolouring and adopt active carbon, its addition is 3% of rice protein powder weight, 50 ~ 90 ℃ of decolourings, processes 10-130min.Adopt 150 ℃ of EATs, 75 ℃ of leaving air temps, homogenization pressure 25MPa carries out spray-drying, makes the feeding method of rice protein hydrolysate of product.
Embodiment 3
Take rice protein powder 1000kg, mix with 40 ℃ of warm water of 5000kg in reactor, add alkali protease to be dissolved, its addition is the 30U/g rice protein powder; Then use phosphorus acid for adjusting pH value 6.5, add complex enzyme formulation, making its mass percentage concentration in solution is 0.6%, at 40 ℃ of hydrolysis 4h of temperature; Complex enzyme formulation used is: alkali protease, neutral proteinase, papain, flavor protease are with composite the forming of 1 ︰ 1.3 ︰ 0.5:0.1 weight portion.Then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment, filter decolouring Bian active carbon, its addition is 4% of rice protein powder weight, 50-90 ℃ of decolouring, processes 10-130min.Adopt 160 ℃ of EATs, 80 ℃ of leaving air temps, homogenization pressure 20MPa carries out spray-drying, makes the feeding method of rice protein hydrolysate of product.
Embodiment 4
Take rice protein powder 1000kg, mix with 35 ℃ of warm water of 6500kg in reactor, add alkali protease to be dissolved, its addition is the 30U/g rice protein powder; Then regulate pH value 7.0 with sodium hydroxide solution, add complex enzyme formulation, making its mass percentage concentration in solution is 0.8%, at temperature 45 C hydrolysis 6.5h, complex enzyme formulation used is: alkali protease, neutral proteinase, papain, flavor protease are with composite the forming of 1 ︰ 1.8 ︰ 0.4 ︰ 0.2 weight portion.Then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment, filter decolouring Bian active carbon, its addition is 3% of rice protein powder weight, 50-90 ℃ of decolouring, processes 10-130min.Adopt 155 ℃ of EATs, 83 ℃ of leaving air temps, homogenization pressure 25 MPa carry out spray-drying, make the feeding method of rice protein hydrolysate of product.
The above raw materials used commercially available product that is, various enzymes are purchased from Novozyme company.
Claims (2)
1. the industrialized preparing process of a feeding method of rice protein hydrolysate, is characterized in that, by following steps, made:
(1) dissolve: take rice protein powder, mix with 10-50 ℃ of warm water in reactor, rice protein powder and water solid-liquid weight ratio 1 ︰ 2-10; Add alkali protease to be dissolved, its addition is the 20-50U/g rice protein powder;
(2) hydrolysis: step (1) gained solution is regulated pH value 5.0-10.0, adds complex enzyme formulation, and making its mass percentage concentration in solution is 0.2%-1.4%, at temperature 30-70 ℃, and hydrolysis 1-13h;
Complex enzyme formulation is that alkali protease, neutral proteinase, papain, flavor protease are with composite the forming of 1 ︰ 1-2 ︰ 0.1-0.5 ︰ 0.1-0.2 weight ratio;
(3) then carry out enzyme-deactivating and filtration, decolouring, spray drying treatment: the decolorizer used that decolours is active carbon, and the 1%-4% that its addition is rice protein powder weight processes 10-130min at 50-90 ℃; Adopt EAT 130-160 ℃, leaving air temp 60-85 ℃, homogenization pressure 10-30MPa carries out spray-drying, obtains the feeding method of rice protein hydrolysate of powdery.
2. the industrialized preparing process of feeding method of rice protein hydrolysate according to claim 1, is characterized in that, Optimizing Technical is: in dissolving step, the weight ratio of rice protein powder and water is 1 ︰ 5; Add complex enzyme formulation in hydrolysing step, making its mass percentage concentration in solution is 0.6%, water transfer solution pH value 6.5,40 ℃ of hydrolysis temperatures, hydrolysis time 4h.
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Cited By (3)
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| CN103766609A (en) * | 2014-02-13 | 2014-05-07 | 哈尔滨伟平科技开发有限公司 | Preparation method of polypeptide feed additive |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN107794289A (en) * | 2017-11-02 | 2018-03-13 | 林峰 | A kind of rice active peptide and preparation method |
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| CN101974590A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Method for extracting rice peptides |
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Cited By (4)
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|---|---|---|---|---|
| CN103766609A (en) * | 2014-02-13 | 2014-05-07 | 哈尔滨伟平科技开发有限公司 | Preparation method of polypeptide feed additive |
| CN103766609B (en) * | 2014-02-13 | 2015-04-22 | 王树海 | Preparation method of polypeptide feed additive |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN107794289A (en) * | 2017-11-02 | 2018-03-13 | 林峰 | A kind of rice active peptide and preparation method |
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Application publication date: 20131204 |