CN103416352A - Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet - Google Patents
Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet Download PDFInfo
- Publication number
- CN103416352A CN103416352A CN2012101640792A CN201210164079A CN103416352A CN 103416352 A CN103416352 A CN 103416352A CN 2012101640792 A CN2012101640792 A CN 2012101640792A CN 201210164079 A CN201210164079 A CN 201210164079A CN 103416352 A CN103416352 A CN 103416352A
- Authority
- CN
- China
- Prior art keywords
- salt
- sugar
- fat diet
- animal model
- hypertension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Non-Alcoholic Beverages (AREA)
Abstract
The invention belongs to the field of medical technology and particularly relates to an animal model of hypertension comprehensively caused by a high-salt, high-sugar and high-fat diet. The animal model is applicable to screening medicines, pharmacological researches and efficacy evaluations during hypertension treatment. On the basis of normal SD rats, the rats on the model group are fed with high-sugar and high-fat feed and meanwhile fed with salt water or high-salt feed every day for 6-17 weeks to obtain the animal models of living hypertension. Model screening index mainly relies on systolic blood pressure assisted by indexes in serum and urine, and influence of the high-salt, high-sugar and high-fat diet on functions of organs such as liver and kidney is monitored.
Description
Technical field
The invention belongs to medical technical field, the hypertension animal model that concrete a kind of high salt, high sugar and high fat diet comprehensively cause, this model can be used for treating screening, pharmacological research and the evaluating drug effect of hypertension Chinese medicine.
Background technology
Hypertension and high salt diet are closely related.China's resident living mode main manifestations is high salt and low potassium, and in diet, salt content is high, and in body, salt load is high.Salt can increase blood volume, causes that capacity raises, and mortality of hypertension increases.And salt can directly destroy vascular wall, accelerate arteriosclerosis, further increase hypertensive hazards.In addition, often the edible food containing high glucose and high fat, can make blood fat, cholesterol raise, and easily forms thrombus, easily causes the body obesity simultaneously.The body obesity, increasing of adipose tissue improved the demand of human body to blood, increased the burden of heart and blood vessel, and the human body blood pressure that must raise could meet the blood supply demand of body.
Still the hypertension animal model that does not have at present standard ground to cause as high glucose and high fat, high salt diet by the simulating human dietary factor.The present invention is according in daily life, human body hypertension complication inducement, and as the salt drink, to increase sugared high lipid food edible etc., set up a kind of animal model that can be used for treating screening, pharmacology and the evaluating drug effect research of hypertension Chinese medicine.
Summary of the invention
The objective of the invention is to set up a kind of preparation method who is used for the treatment of the hypertension animal model of hypertension Chinese medicine screening, pharmacological research and evaluating drug effect.In order to solve the problems of the technologies described above, the present invention is achieved through the following technical solutions: on the basis of normal SD rats, the model group rat is raised the high glucose and high fat feed every day, gives that salt solution is drunk or high salt feed mixed edible simultaneously, 6～17 weeks, can cause hypertension animal model.
The model discrimination index is mainly systolic pressure (SBP), diastolic pressure (DBP), mean pressure (MBP), take systolic pressure (SBP) as main; Survey alanine aminotransferase in serum (ALT), aspartic transaminase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), glucose (GLU), T-CHOL (TC), triglycerides (TG), uric acid (UA), creatinine (Cr) simultaneously; The ion concentration of potassium in serum (K), sodium (Na), chlorine (Cl), total calcium (T-Ca); Survey creatinine (Cr), uric acid (UA) in urine.Be designated as auxiliaryly with serum and urine middle finger, monitor long-term high salt, high sugar and the high fat diet impact on organ functions such as rat blood pressure and liver, kidneys.
Embodiment
Embodiment 1
1 experiment material and instrument
1.1 animal SD rat, male and female half and half, body weight 180～220g, 20.Credit number SCXK (Zhejiang) 20080033, provided by Zhejiang Province's Experimental Animal Center.
1.2 instrument ALC-NIBP non-invasive blood pressure measuring and analysis system (Shanghai Alcott bio tech ltd); Holland's prestige figure SELECTRA-E full automatic biochemical apparatus (Dutch Rittal GmbH); Powerwave340 microplate reader (U.S. Bio-TEK company); DH-500K/Na/Cl/Ca/pH blomelicalbloodgasandelectrolrteanalyzers (Shenzhen Bai Kang founds Science and Technology Ltd.).
1.3 reagent alanine aminotransferase (ALT), aspartic transaminase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), T-CHOL (TC), triglycerides (TG), glucose (GLU), uric acid (UA), creatinine (Cr) (Shanghai Foxing Changzheng medical science Co., Ltd); A calibration/flushing liquor, B slope calibration solution, deproteinized cleaning fluid, reach biosensor technique Co., Ltd and manufacture in Wuhan.
2 experimental techniques
2.1 modeling SD rat, male and female half and half, body weight 180～220g, 20.10 as Normal group, and 10 is model modeling group.Model modeling group is freely drunk 1% salt solution, and high glucose and high fat forage feed (sucrose 5%～15%, lard 5%～15%, cholesterol 0.5%～5%, cholate 0.2%～2%, remain as normal diet) is fed simultaneously, 6～17 weeks.Normal group gives normal diet.Experimental session, normal group is freely drunk water.
2.2 testing index is surveyed respectively rat systolic pressure (SBP), diastolic pressure (DBP), mean pressure (MBP) in 0 week, 6 weeks, 17 weeks in modeling; In 5 weeks, 8 weeks, 10 weeks, 12 weeks of modeling, take a blood sample after fasting 10h respectively, adopt biochemical process to measure the content of alanine aminotransferase (ALT), aspartic transaminase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), T-CHOL (TC), triglycerides (TG), glucose (GLU), uric acid (UA), creatinine (Cr); Survey the ion concentration of potassium in serum (K), sodium (Na), chlorine (Cl), total calcium (T-Ca) simultaneously.In 7 weeks, 9 weeks, 11 weeks, 16 weeks of modeling, rat is put into to metabolic cage, freely drink water, detect the content of creatinine (Cr), uric acid (UA) in urine.
2.3 statistical analysis measurement data data with
Mean, relatively adopt the t-test check between group; Categorical data adopts X
2Check.
3 experimental results
3.1 the impact of modeling factor on the SD rat blood pressure
As shown in Table 1, with Normal group, compare, modeling starts on the 6th week, and model modeling group rat systolic pressure (SBP) is rising (P<0.05) obviously.
Table 1 systolic pressure statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
As shown in Table 2, with Normal group, compare, in the time of 17 weeks, there were significant differences for model modeling group rat diastolic pressure (DBP) (P<0.01).
Table 2 diastolic pressure statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
As shown in Table 3, with Normal group, compare, in the time of 17 weeks, there were significant differences for model modeling group rat average pulse pressure (MBP) (P<0.05).
Table 3 is the pulse pressure statistical form all
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.2 the impact of modeling factor on SD rat creatinine (Cr)
As shown in Table 4, with Normal group, compare, in the time of 12 weeks, model modeling group rat creatinine (Cr) obviously raises (P<0.05), points out 12 weeks beginning modeling factors to make rat Renal tissues damage occur.
Table 4 creatinine (Cr) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.3 the impact of modeling factor on SD rat alanine aminotransferase (ALT)
As shown in Table 5, with Normal group, compare obviously rising (P<0.01) of model modeling group rat alanine aminotransferase (ALT) in the time of 12 weeks.
Point out 12 weeks beginning modeling factors to make rat liver tissue injury occur.
Table 5 alanine aminotransferase (ALT) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.4 the impact of modeling factor on SD rat aspartic transaminase (AST)
As shown in Table 6, with Normal group, compare obviously rising (P<0.01) of model modeling group rat aspartic transaminase (AST) in the time of 12 weeks.
Point out 12 weeks beginning modeling factors to make rat liver tissue injury occur.
Table 6 aspartic transaminase (AST) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.5 the impact of modeling factor on SD rat gamma glutamyl transpeptidase (GGT)
As shown in Table 7, with Normal group, compare obviously rising (P<0.05) of model modeling group rat gamma glutamyl transpeptidase (GGT) in the time of 12 weeks.
Point out 12 weeks beginning modeling factors to make rat liver tissue injury occur.
Table 7 gamma glutamyl transpeptidase (GGT) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.6 the impact of modeling factor on SD rat alkaline phosphatase (ALP)
As shown in Table 8, with Normal group, compare obviously rising (P<0.05) of model modeling group rat alkaline phosphatase (ALP) in the time of 10 weeks.Point out 10 weeks beginning modeling factors to make rat liver tissue injury occur.
Table 8 alkaline phosphatase (ALP) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05, P
*<0.01
3.7 the impact of modeling factor on SD rat glucose (GLU)
As shown in Table 9, with Normal group, compare, in the time of 12 weeks, model modeling group rat glucose (GLU) is rising (P<0.01) obviously.While pointing out 12 weeks, the modeling factor raises blood sugar in rat blood serum.
Table 9 glucose (GLU) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.8 the impact of modeling factor on SD rat triglycerides (TG)
As shown in Table 10, with Normal group, compare, in the time of 10 weeks, model modeling group rat triglycerides (TG) is rising (P<0.05) obviously.While pointing out 10 weeks, it is abnormal that lipid metabolism appears in the modeling factor.
Table 10 triglycerides (TG) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.9 the impact of modeling factor on SD rat cholesterol (TC)
As shown in Table 11, with Normal group, compare, in the time of 10 weeks, model modeling group rat cholesterol (TC) is rising (P<0.01) obviously.While pointing out 10 weeks, it is abnormal that lipid metabolism appears in the modeling factor.
Table 11 cholesterol (TC) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.10 the impact of modeling factor on SD rat uric acid (UA)
As shown in Table 12, with Normal group, compare obviously rising (P<0.01) of model modeling group rat uric acid (UA) in the time of 10 weeks.Point out 10 weeks beginning modeling factors to make renal tissues of rats damage occur.
Table 12 uric acid (UA) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.11 the impact of modeling factor on potassium ion (K) in the SD rat blood serum
As shown in Table 13, with Normal group, compare, in the time of 12 weeks, model modeling group rat potassium ion (K) obviously reduces (P<0.05).Point out 12 weeks beginning modeling factors that rat blood serum K ion is occurred extremely.
Table 13 potassium ion (K) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.12 the impact of modeling factor on sodium ion (Na) in the SD rat blood serum
As shown in Table 14, with Normal group, compare obviously rising (P<0.05) of model modeling group rat sodium ion (Na) in the time of 12 weeks.Point out 12 weeks beginning modeling factors that rat blood serum Na ion is occurred extremely.
Table 14 sodium ion (Na) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.13 the impact of modeling factor on chlorion (Cl) in the SD rat blood serum
As shown in Table 15, with Normal group, compare obviously rising (P<0.05) of model modeling group rat chlorion (Cl) in the time of 12 weeks.Point out 12 weeks beginning modeling factors that rat blood serum Cl ion is occurred extremely.
Table 15 chlorion (Cl) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.14 the impact of modeling factor on total calcium ion (T-Ca) in the SD rat blood serum
As shown in Table 16, with Normal group, compare, in the time of 10 weeks, the total calcium ion of model modeling group rat (T-Ca) obviously reduces (P<0.05).Point out 10 weeks beginning modeling factors that rat blood serum T-Ca ion is occurred extremely.
Total calcium ion (T-Ca) statistical form of table 16
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.15 the impact of modeling factor on creatinine (Cr) in the SD rat urine
As shown in Table 17, with Normal group, compare, in the time of 16 weeks, model modeling group rat urine creatinine (Cr) obviously reduces (P<0.05).While pointing out 16 weeks, the renal tissues of rats internal organs have damage.
Table 17 urine creatinine (Cr) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
3.16 the impact of modeling factor on uric acid (UA) in the SD rat urine
As shown in Table 18, with Normal group, compare obviously reduction (P<0.01) of uric acid (UA) in model modeling group rat urine in the time of 9 weeks.While pointing out 9 weeks, the renal tissues of rats internal organs have damage.
Uric acid in table 18 urine (UA) statistical form
N=10)
Annotate: with Normal group, compare,
*P<0.05,
*P<0.01
Above-mentioned the results show, the present invention adopts salt solution to drink to increase sugared high lipid food to feed modeling, and after 8～17 weeks, rat model is compared with normal rat, can occur that systolic pressure obviously raises; Creatinine, alanine aminotransferase, aspartic transaminase, gamma glutamyl transpeptidase, alkaline phosphatase, glucose, triglycerides, cholesterol, uric acid, sodium ion, chlorion raise; In blood, potassium ion, total calcium ion reduce; In urine, creatinine, uric acid obviously reduce.The hypertension animal model that the present invention sets up meets the mankind's pathogenesis, for the carrying out of screening, pharmacological research and evaluating drug effect of next step treatment hypertension Chinese medicine laid a good foundation.
Claims (6)
1. the hypertension animal model that high salt, high sugar and high fat diet comprehensively cause, the model group rat is raised the high glucose and high fat feed every day, gives that salt solution is drunk or high salt feed simultaneously, 6～17 weeks, can cause the liveliness proof hypertension animal model.
2. the hypertension animal model comprehensively caused according to claim 1 high salt, high sugar and high fat diet is characterized in that: the common SD rat of take is laboratory animal.
3. the hypertension animal model comprehensively caused according to claim 1 high salt, high sugar and high fat diet, is characterized in that the formula composition of high glucose and high fat feed comprises normal diet, sucrose, lard, cholesterol, no. 3 bile salt.Concrete ratio: sucrose 5%～15%, lard 5%～15%, cholesterol 0.5%～5%, cholate 0.2%～2% remain as normal diet.
4. the hypertension animal model comprehensively caused according to claim 1 high salt, high sugar and high fat diet, it is characterized in that high salt can be by adding salt or freely drinking the salt solution realization in feed, it is 0.3～2.5% that the concentration of freely drinking salt solution is selected volumetric concentration.
5. the hypertension animal model comprehensively caused according to claim 1 high salt, high sugar and high fat diet, is characterized in that the time that high salt, high sugar and high fat diet are raised is 6～17 weeks.
6. the hypertension animal model comprehensively caused according to claim 1 high salt, high sugar and high fat diet, is characterized in that the model discrimination index is mainly systolic pressure, diastolic pressure, mean pressure, take systolic pressure as main; It is auxiliary surveying creatinine, uric acid content etc. in the content of alanine aminotransferase in serum, aspartic transaminase, gamma glutamyl transpeptidase, alkaline phosphatase, glucose, T-CHOL, triglycerides, uric acid, creatinine, potassium, sodium, chlorine, total calcium ion and urine simultaneously, monitors long-term high salt, high sugar and the high fat diet impact on organ functions such as rat blood pressure and liver, kidneys.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101640792A CN103416352A (en) | 2012-05-22 | 2012-05-22 | Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101640792A CN103416352A (en) | 2012-05-22 | 2012-05-22 | Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103416352A true CN103416352A (en) | 2013-12-04 |
Family
ID=49642161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101640792A Pending CN103416352A (en) | 2012-05-22 | 2012-05-22 | Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103416352A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947876A (en) * | 2014-04-18 | 2014-07-30 | 兰州大学 | Feed for laboratory rats and mice and preparation method thereof |
| CN113655134A (en) * | 2021-07-06 | 2021-11-16 | 杭州师范大学 | Metabonomics analysis method for treating hypertension caused by high-fat diet in rat serum |
| CN114831078A (en) * | 2022-06-09 | 2022-08-02 | 辽宁中医药大学 | Establishment of hypertension yin deficiency syndrome and/or hypertension non-yin deficiency syndrome animal model by fecal bacteria transplantation method, evaluation and application |
| CN115349491A (en) * | 2022-07-25 | 2022-11-18 | 山东中医药大学 | Method for establishing animal model of normal high blood pressure phlegm-dampness accumulation syndrome |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887303A (en) * | 2005-12-31 | 2007-01-03 | 安徽医科大学 | Application of alcohol extract of spanishneedles herb in preparing medicine for preventing and treating non-alcoholic fatty liver |
| CN101199504A (en) * | 2007-11-21 | 2008-06-18 | 深圳海王药业有限公司 | Function of stilbene oxygen acid or salt in preparing antiatheroscloresis medicament |
| CN101766149A (en) * | 2010-01-29 | 2010-07-07 | 东南大学 | Preparation method of 2-type diabetic nephropathy model |
| CN102334465A (en) * | 2010-12-28 | 2012-02-01 | 四川大学华西医院 | Method for establishing acute Stanford A-type aortic dissection MODS animal model |
| CN102858958A (en) * | 2010-02-03 | 2013-01-02 | 日本国立癌症研究中心 | Induced Hepatic Stem Cell And Process For Production Thereof, And Applications Of The Cell |
-
2012
- 2012-05-22 CN CN2012101640792A patent/CN103416352A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887303A (en) * | 2005-12-31 | 2007-01-03 | 安徽医科大学 | Application of alcohol extract of spanishneedles herb in preparing medicine for preventing and treating non-alcoholic fatty liver |
| CN101199504A (en) * | 2007-11-21 | 2008-06-18 | 深圳海王药业有限公司 | Function of stilbene oxygen acid or salt in preparing antiatheroscloresis medicament |
| CN101766149A (en) * | 2010-01-29 | 2010-07-07 | 东南大学 | Preparation method of 2-type diabetic nephropathy model |
| CN102858958A (en) * | 2010-02-03 | 2013-01-02 | 日本国立癌症研究中心 | Induced Hepatic Stem Cell And Process For Production Thereof, And Applications Of The Cell |
| CN102334465A (en) * | 2010-12-28 | 2012-02-01 | 四川大学华西医院 | Method for establishing acute Stanford A-type aortic dissection MODS animal model |
Non-Patent Citations (2)
| Title |
|---|
| 刘晓宇等: "长期高脂、高糖、高盐饮食对大鼠热和机械痛阈的影响", 《中国临床药理学与治疗学》 * |
| 夏燕萍等: "高糖高脂饮食诱导建立SD大鼠代谢综合征模型", 《中国现代医学杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947876A (en) * | 2014-04-18 | 2014-07-30 | 兰州大学 | Feed for laboratory rats and mice and preparation method thereof |
| CN113655134A (en) * | 2021-07-06 | 2021-11-16 | 杭州师范大学 | Metabonomics analysis method for treating hypertension caused by high-fat diet in rat serum |
| CN114831078A (en) * | 2022-06-09 | 2022-08-02 | 辽宁中医药大学 | Establishment of hypertension yin deficiency syndrome and/or hypertension non-yin deficiency syndrome animal model by fecal bacteria transplantation method, evaluation and application |
| CN114831078B (en) * | 2022-06-09 | 2023-08-11 | 辽宁中医药大学 | Fecal bacteria transplanting method for establishing animal model, evaluation and application of hypertension yin deficiency syndrome and/or hypertension non-yin deficiency syndrome |
| CN115349491A (en) * | 2022-07-25 | 2022-11-18 | 山东中医药大学 | Method for establishing animal model of normal high blood pressure phlegm-dampness accumulation syndrome |
| CN115349491B (en) * | 2022-07-25 | 2023-09-15 | 山东中医药大学 | Method for establishing normal high-value blood pressure phlegm-dampness accumulation animal model |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sarica et al. | Role of overweight status on stone-forming risk factors in children: a prospective study | |
| Cantor et al. | A comparative serial echocardiographic analysis of cardiac structure and function in rats subjected to pressure or volume overload | |
| CN103385202A (en) | Preparation method for living hypertension animal model caused by high-sugar and high-fat feed feeding | |
| Sarıca et al. | Effect of being overweight on stone-forming risk factors | |
| CN103416352A (en) | Animal model of hypertension comprehensively caused by high-salt, high-sugar and high-fat diet | |
| CN103125439A (en) | Method for building animal model with hyperuricemia-combined diabetes | |
| Smith et al. | Pathophysiological and physicochemical basis of ammonium urate stone formation in dolphins | |
| CN102640725A (en) | Preparation method of rat model with hypertension complicated by hyperlipidemia | |
| CN103416347A (en) | Lifestyle hypertension animal model caused by drinking and high-sugar high-fat diet | |
| Seo et al. | Which metabolic syndrome criteria best predict the presence of non-alcoholic fatty liver disease? | |
| Gursoy et al. | The effects of apricot on serum proteins and liver enzymes in rats | |
| CN104698160B (en) | A kind of test kit for assessing male's twenty-four-hour urine sodium value | |
| CN102511447B (en) | Blood stasis-YANG hyperactivity-Phlegm hypertensive animal model and preparation method thereof | |
| CN104569437B (en) | For assessing the test kit of women twenty-four-hour urine sodium value | |
| Chen et al. | Association of P-Wave dispersion with overall and cardiovascular mortality in hemodialysis patients | |
| CN103815128B (en) | High-calorie feed for establishing atherosclerotic animal model and application thereof | |
| CN103735550B (en) | A kind of pharmaceutical composition and application thereof for the treatment of chronic kidney disease myocardial damage | |
| WO2011004009A1 (en) | Use of creatinine as dilution agent to determine the bodily composition of a mammal | |
| CN103380760A (en) | Preparation method of model for animals with living hypertension due to drinking | |
| Soares et al. | Thiobarbituric acid reactive substances in dogs with spontaneous hypercortisolism | |
| CN104569438B (en) | A kind of test kit for assessing women twenty-four-hour urine sodium value | |
| CN103385215A (en) | Preparation method for living hypertension animal model caused by high-purine feed feeding | |
| CN102920991B (en) | Application of composition of Chinese yarn polysaccharide and Chinese yarn protein | |
| CN105136780B (en) | Application of secretion factor GREM2 in preparation of obesity screening reagent | |
| Sahin et al. | Is there a correlation between subjective global assessment and food intake, anthropometric measurement and biochemical parameters in nutritional assessment of haemodialysis patients |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131204 |