CN103409328B - Rhodotorula mucilaginosa and application thereof in degradation and decoloring of dyes and production of carotenoid - Google Patents
Rhodotorula mucilaginosa and application thereof in degradation and decoloring of dyes and production of carotenoid Download PDFInfo
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Abstract
The invention discloses a Rhodotorula mucilaginosa and the application thereof in the degradation and decoloring of dyes and the production of carotenoid. The Preservation No. of the Rhodotorula mucilaginosa JM401 is CCTCC NO: M 2013088; the saccharomycete can be used for carrying out degradation and decoloring on a triphenlmethane dye: and centrifugally collecting activated seed solution bacteria and vaccinating the bacteria to a decoloring medium containing the dye, and carrying out shake cultivation on the obtained product for 2-12 h at a temperature of 25-37 DEG C and a pH value of 4.0-7.5 so as to obtain a decoloring culture solution; and the saccharomycete can be used for producing carotenoids by fermentation: taking an activated seed solution and vaccinating the activated seed solution to a fermentation medium, and carrying out fermentation cultivation on the obtained product for 36-48 h at a temperature of 25-32 DEG C and a pH value of 5.0-7.0 so as to obtain fermentation liquor containing the carotenoids. The Rhodotorula mucilaginosa disclosed by the invention simultaneously has the abilities of carrying out efficient decoloring on triphenlmethane dyes and carrying out high-yield production on carotenoids, therefore, the Rhodotorula mucilaginosa has an extremely broad application prospect in a field to which the Rhodotorula mucilaginosa belongs.
Description
Technical field
The present invention relates to a strain rhodotorula mucilaginosa and the application in dye degrades decolouring and fermentative carotenoid are produced thereof, belong to microorganism and fermentation technical field.
Background technology
Dyestuff is a kind of important pollutent.The existing 100,000 kinds of dyestuffs in the whole world, annual output is about 0.75 ~ 1 × 106 tons, wherein has the dyestuff of 10 ~ 15% with discharge of wastewater in environment.Dyestuff has hormesis to eye and Digestive tract, can cause skin sensitivity pain, even causes canceration to occur; In addition, the colourity of water can be affected after dyestuff drains into water body, the transmission of interference sunlight, cause the ecosystem disorderly; Some dyes also produces cytotoxicity to hydrocoles such as fishes.Therefore, residual dyestuff in the environment must be removed.
Triphenylmethane dye is the third-largest dyestuff that usage quantity is only second to azoic dyestuff, anthraquinone dye, is widely used in the industry such as textile printing and dyeing, papermaking, process hides, but studies less.The triphenylmethane dye that discovered in recent years represents with Viola crystallina has mammalian cell poisons and carcinogenic, teratogenesis and mutagenic effect, is environmental pollutant human health to potential hazard.Utilize microbiological treatment dyestuff to have power consumption is low, cost is low, sustainability is good etc. advantage, therefore screens microorganism triphenylmethane dye to degradation and decolorization effect, for triphenylmethane dye Pollution abatement and human health significant.
Rhodotorula mucilaginosa is that a kind of fermentation produces the unicellular eukaryote of carotenoid, and its lag phase is short, and growth rapidly, is that the fungi of the other types such as the mould of other types is incomparable.At present, existing investigator utilizes rhodotorula mucilaginosa to produce the report of carotenoid, but then there is not been reported for the application of triphenylmethane dye to utilize rhodotorula mucilaginosa efficient degradation to decolour.
Summary of the invention
In view of the foregoing defects the prior art has, an object of the present invention is to provide a strain rhodotorula mucilaginosa (Rhodotorula mucilaginosa) JM401, and deposit number is CCTCC NO:M 2013088.
Another object of the present invention is to provide the application of above-mentioned rhodotorula mucilaginosa in triphenylmethane dye degradation and decolorization, and is realized by following steps: described rhodotorula mucilaginosa is carried out slant culture and seed culture, obtains activated seed liquid; Collected by centrifugation thalline, and be transferred to isopyknic containing in the decolouring substratum of triphenylmethane dye with described seed liquor, in 25 ~ 37 DEG C, shaking culture 2 ~ 12h under the condition of pH4.0 ~ 7.5, shaking speed 150 ~ 200r/min, obtain decolouring nutrient solution; The initial concentration of described triphenylmethane dye is 10 ~ 200mg/L, and described decolouring medium component is counted with g/L: peptone 8 ~ 12, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, and all the other compositions are water.
Another object of the present invention is to provide the application of above-mentioned rhodotorula mucilaginosa in fermentative production carotenoid, and is realized by following steps: described rhodotorula mucilaginosa is carried out slant culture and seed culture, obtains activated seed liquid; Described seed liquor is seeded to fermention medium by the inoculum size of 2% by volume, and in 25 ~ 32 DEG C, the condition bottom fermentation of pH5.0 ~ 7.0 cultivates 36 ~ 48h, obtains the fermented liquid containing carotenoid; Described fermentation medium components is counted with g/L: glucose 10 ~ 12, peptone 10 ~ 12, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, and all the other compositions are water.
Its further technical scheme is:
In above-mentioned application, described slant culture temperature is 32 DEG C, incubation time 40h, and slant culture based component counts peptone 10 ~ 12 with g/L, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, agar 12 ~ 14, all the other compositions are water, pH5.5 ~ 6.0,121 DEG C of sterilizing 15min; Described seed culture temperature 32 DEG C, incubation time 20h, seed culture based component counts peptone 10 ~ 12 with g/L, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, magnesium sulfate heptahydrate 5 ~ 7, and all the other compositions are water, pH5.5 ~ 6.0,121 DEG C of sterilizing 15min.
Rhodotorula mucilaginosa provided by the present invention (Rhodotorula mucilaginosa) JM401, be preserved in China typical culture collection center (CCTCC), deposit number on March 18th, 2013: CCTCC NO:M2013088, address: China, Wuhan, Wuhan University.This bacterial strain is hereinafter referred to as rhodotorula mucilaginosa JM401CCTCC M 2013088.
Beneficial effect of the present invention is as follows:
The invention provides a kind of rhodotorula mucilaginosa JM401 CCTCC M 2013088, under certain processing condition, this rhodotorula mucilaginosa has efficient significant degradation and decolorization effect to triphenylmethane dye (taking Viola crystallina as typical case), has the ability of High Yield of Carotenoid concurrently simultaneously; After testing, when Viola crystallina initial concentration is 50mg/L, cultivate dye degrades percent of decolourization in 37 DEG C through 2h decolouring and can reach 95.21%, cultivating dye degrades percent of decolourization in 30 DEG C through 6h decolouring can reach 95.54%, cultivates 48h carotenoid output reach 7.12mg/L in 28 DEG C of condition bottom fermentations; Prove that rhodotorula mucilaginosa of the present invention all has very wide application prospect in triphenylmethane dye Pollution abatement field and carotenoid industrial fermentation production field.
Embodiment
By the following examples the present invention is specifically described.
If no special instructions, each raw material and reagent involved by following examples are the domestic or pure commodity of Import Analysis, relate to experimental technique and are this area ordinary method.
Slant culture described in following examples and seed culture technique as follows:
(1) slant culture: rhodotorula mucilaginosa JM401 CCTCC M 2013088 is inoculated on slant medium, cultivate 40h in 32 DEG C, slant culture based component is counted with g/L: peptone 11, yeast extract 5, sodium-chlor 5, agar 12, all the other compositions are water, pH5.5,121 DEG C of sterilizing 15min.
(2) seed culture: the bacterial classification transfering loop that slant medium grows is got a ring and is seeded in seed culture medium, in 32 DEG C of shaking culture 20h, obtain activated seed liquid, seed culture based component is counted with g/L: peptone 11, yeast extract 6, sodium-chlor 5, magnesium sulfate heptahydrate 6, all the other compositions are water, pH5.5, the above-mentioned seed culture medium of the bottled 100ml of each 250ml triangle, 121 DEG C of sterilizing 15min.
The screening of rhodotorula mucilaginosa JM401 CCTCC M 2013088, Isolation and ldentification
The screening and separating of embodiment 1 rhodotorula mucilaginosa JM401 CCTCC M 2013088
Rhodotorula mucilaginosa JM401 CCTCC M 2013088 is separated in October, 2011 and produces offal from Luzhou, Sichuan; Get 5g offal and be placed in 250ml sterilizing triangular flask containing 10 little granulated glass spherees, add 100ml stroke-physiological saline solution, with 150r/min shaking culture 20min on 30 DEG C of shaking tables, leave standstill 2h; Get above-mentioned bacteria suspension, diluted 10
5, 10
6, 10
7doubly, get 100 μ l to be respectively coated on water-tough treatment flat board (water-tough treatment flat board composition is in g/L: peptone 10, yeast extract 5, sodium-chlor 5, aniline blue 2, agar 15, all the other compositions are water, pH5.5,121 DEG C of sterilizing 15min), each concentration is in triplicate, 40h is cultivated in 32 DEG C, select the single bacterium colony repeatedly purifying that transparent circle is larger, by the inoculation after purifying on slant medium, be put in 4 DEG C of Refrigerator stores after cultivating 48h in 30 DEG C stand-by.
By inoculation stand-by for 4 DEG C of Refrigerator stores in seed culture medium, after cultivating 20h in 32 DEG C, get 40ml activated seed liquid centrifugal, collect whole thalline and transferred in 40ml decolouring substratum, in mensuration culturing process, bacterial strain is to the degradation and decolorization rate of dyestuff, therefrom filter out the bacterial strain that a strain has efficient degradation decoloring ability, name as JM401.
The qualification of embodiment 2 rhodotorula mucilaginosa JM401 CCTCC M 2013088
(1) morphological specificity
After slant medium cultivates 40h, microscopic examination: colony diameter is about 2mm, ovalize projection, neat in edge, smooth surface, in viscous, produces carotenoid pigment; In seed culture medium, strain growth 20h reaches logarithmic phase; At 32 DEG C, growing state is best.
(2) ITS-5.8S rDNA sequential analysis
Object bacterial strain is cultivated 24h on slant medium, round pcr is utilized to increase the ITS-5.8S rDNA sequence of this bacterium, its Oligonucleolide primers is: TCCGTAGGTGAACCTGCGG (ITS1) and TCCTCCGCTTATTGATATGC (ITS4), amplified production transfers to Shanghai Sangon Biological Engineering Technology And Service Co., Ltd to check order, and its sequence is as shown in SEQ ID NO:1.ITS-5.8S rDNA sequence known in sequencing result and GeneBank is carried out tetraploid rice, and finding with the homology of Rhodotorula mucilaginosa strain ATCC66034 (EU853846.1) the highest, is 100%.
(3) physiological and biochemical property
According to " saccharomycetic feature and identification handbook " (Barney top grade work, Hu Ruiqing translates, press of Qingdao Marine University, 1991) and " yeast production and application manual " (Yu Jingzhi etc., China Light Industry Press, 2005) carry out Physiology and biochemistry qualification to bacterial strain JM401, its major physiological biochemical character is see table 1.Its physiological and biochemical property and rhodotorula mucilaginosa match.
Table 1 rhodotorula mucilaginosa JM401 CCTCC M 2013088 Physiology and biochemistry is identified
| Feature | JM401 | Feature | JM401 |
| Cell: diameter 3 ~ 5 μm | + | Utilization of carbon source: D-semi-lactosi | - |
| Circle or oval | + | Sucrose | + |
| Budding | + | Maltose | + |
| Pseudohypha | - | Lactose | - |
| Ballistospore | - | Raffinose | + |
| Bacterium colony: oval | + | D-wood sugar | + |
| Neat in edge, smooth surface | + | Melizitose | + |
| Cement shape, slightly epirelief | + | Starch | - |
| Produce carotenoid pigment | + | Erythritol | - |
| Growth: do not add vitamin H | + | D-glyconic acid-1,5-lactone | + |
| 30℃ | + | D-glucuronic acid | - |
| Add 0.1% actidione | - | Methyl alcohol | - |
| Only nitrogen source: nitrate | - | Other: Starch formation | - |
| Cadaverine | - | Hydrolysis of urea | + |
Note: "-" represents negative; "+" represents positive.
In conjunction with above-mentioned morphological specificity, genetics qualification and Physiology and biochemistry qualification, determine that this bacterial strain is rhodotorula mucilaginosa (Rhodotorula mucilaginosa).
Rhodotorula mucilaginosa JM401 CCTCC M 2013088 degradation and decolorization Viola crystallina
The medium component that decolours described in following examples is counted with g/L: peptone 11, yeast extract 6, sodium-chlor 6, and all the other compositions are water, the decolouring substratum that the bottled 40ml of each 100ml triangle is fresh, 121 DEG C of sterilizing 15min.The initial concentration of the Viola crystallina when single batch of decolouring in degradation and decolorization system is 10 ~ 200mg/L, and when cultured continuously decolouring application, in each degradation and decolorization culture system, the initial concentration of Viola crystallina is 50mg/L.
The measuring method of described Viola crystallina degradation and decolorization rate is: the ratio of getting decolouring nutrient solution 1:2 by volume adds dehydrated alcohol, mixing, and the centrifugal 10min of 8000r/min, gets supernatant liquor; Measure the concentration of excess dye in supernatant liquor with spectrophotometer at the maximum absorption band place (589nm) of dyestuff, and measure the degradation and decolorization rate of dyestuff with the decolouring substratum not inoculating described rhodotorula mucilaginosa as a control group; Often organize experiment three parallel, degradation and decolorization rate calculates as follows:
Embodiment 3 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH6.0, in 37 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 92.35%.
Embodiment 4 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH5.5, in 37 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 95.21%.
Embodiment 5 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH6.0, in 30 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 86.13%.
Embodiment 6 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH7.0, in 25 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 81.38%.
Embodiment 7 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH7.5, in 25 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 74.53%.
Embodiment 8 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH4.0, in 25 DEG C of shaking culture 2h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 85.09%.
Embodiment 9 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH5.5, in 30 DEG C of shaking culture 6h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 95.54%.
Embodiment 10 single batches of degradation and decolorization are cultivated
Get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH6.0, in 30 DEG C of shaking culture 12h, shaking speed 150r/min, obtains decolouring nutrient solution.The degradation and decolorization rate recording Viola crystallina reaches 91.26%.
Embodiment 11 continuous batches of degradation and decolorization are cultivated
First batch: get cultured activated seed liquid 40ml, load in the sterile centrifugation tube of two 50ml, each centrifuge tube dress 20ml, in the centrifugal 10min of 8000r/min, abandons supernatant, collect whole thalline and be all forwarded in 100ml triangular flask, the decolouring substratum that 40ml initial concentration is 50mg/L Viola crystallina is wherein housed, pH5.0, in 30 DEG C of shaking culture 2h, shaking speed 150r/min, obtains first batch of decolouring nutrient solution.
Second batch: in first batch of decolouring nutrient solution, add the Viola crystallina that final concentration is 50mg/L (after first batch of decolouring, remain Viola crystallina content in decolouring system and be defaulted as zero), in 30 DEG C, shake shaking culture 2h is continued under the condition of pH5.0, shaking speed 150r/min, obtains second batch of decolouring nutrient solution.
3rd batch: repeat the second batch operation.
By that analogy, the continuous stripping carrying out the 4th to the tenth batch is cultivated, and each batch of Viola crystallina decolorizing effect that continuous stripping is cultivated is as shown in table 3.
Table 2 Viola crystallina continuous batch of degradation and decolorization effect
| Batch | Degradation and decolorization rate (%) | Batch | Degradation and decolorization rate (%) |
| 1 | 94.20 | 6 | 88.72 |
| 2 | 93.69 | 7 | 84.49 |
| 3 | 91.94 | 8 | 81.87 |
| 4 | 91.55 | 9 | 79.16 |
| 5 | 90.11 | 10 | 75.32 |
Rhodotorula mucilaginosa JM401CCTCC M 2013088 fermentative production carotenoid
Described in following examples, fermentation medium components is counted with g/L: glucose 11, peptone 11, yeast extract 5, sodium-chlor 5, and all the other compositions are water, 121 DEG C of sterilizing 15min.
The measuring method of described carotenoid content is: get 5ml fermented liquid, leaves heart 10min, abandon supernatant in 3500, and repeated centrifugation after thalline washing, gets and be deposited in 55 DEG C and dry to constant weight; Precise 0.1g dry mycelium also adds 6mL3mol/L hydrochloric acid, 1h is extracted in room temperature concussion, cool rapidly after boiling water bath 4min, heart 10min is left in 3500, the mixed solution (volume ratio 1:1) getting precipitation sherwood oil and acetone is colourless to thalline in room temperature concussion extraction 2 ~ 3 times, collect centrifugate and be Extraction of carotenoid pigment liquid, measure the absorbancy of 453nm place extracting solution, and be calculated as follows the output of carotenoid:
Carotenoid output (mg/L)=biomass (g/L) × carotenoid content (mg/g)
Carotenoid content (mg/g)=(OD
453× D × V)/0.16W
Wherein OD
453for extracting solution is in the absorbancy at 453nm place, D is extension rate, and V extracts the volume (mL) of solvent for use, and 0.16 is the molar extinction coefficient (mol of carotenoid
-1cm
-1), W is yeast dry cell weight (g).
Embodiment 12
Get cultured activated seed liquid 2ml, be transferred in the 250ml triangular flask containing 100ml fermention medium, Medium's PH Value is 6.0, cultivates 48h in 25 DEG C of condition bottom fermentations, obtains the fermented liquid containing carotenoid.Recording carotenoid content is 5.46mg/L.
Embodiment 13
Get cultured activated seed liquid 2ml, be transferred in the 250ml triangular flask containing 100ml fermention medium, Medium's PH Value is 6.5, cultivates 48h in 28 DEG C of condition bottom fermentations, obtains the fermented liquid containing carotenoid.Recording carotenoid content is 7.12mg/L.
Embodiment 14
Get cultured activated seed liquid 2ml, be transferred in the 250ml triangular flask containing 100ml fermention medium, Medium's PH Value is 7.0, cultivates 36h in 32 DEG C of condition bottom fermentations, obtains the fermented liquid containing carotenoid.Recording carotenoid content is 5.86mg/L.
Embodiment 15
Get cultured activated seed liquid 2ml, be transferred in the 250ml triangular flask containing 100ml fermention medium, Medium's PH Value is 5.0, cultivates 48h in 32 DEG C of condition bottom fermentations, obtains the fermented liquid containing carotenoid.Recording carotenoid content is 7.01mg/L.
In sum, the ability of rhodotorula mucilaginosa JM401 CCTCC M 2013088 combining efficient decolouring triphenylmethane dye (Viola crystallina) provided by the invention and High Yield of Carotenoid.
Described in upper is only the preferred embodiment of the present invention, the invention is not restricted to above embodiment.Be appreciated that other improvement that those skilled in the art directly derive without departing from the spirit and concept in the present invention or associate and change, all should think and be included within protection scope of the present invention.
Claims (8)
1. a strain rhodotorula mucilaginosa (Rhodotorula mucilaginosa) JM401, deposit number is CCTCCNO:M 2013088.
2. the application of rhodotorula mucilaginosa described in claim 1 in Viola crystallina degradation and decolorization.
3. the application of rhodotorula mucilaginosa in Viola crystallina degradation and decolorization according to claim 2, be is characterized in that being realized by following steps: described rhodotorula mucilaginosa is carried out slant culture and seed culture, obtains activated seed liquid; Collected by centrifugation thalline, and be transferred to isopyknic containing in the decolouring substratum of Viola crystallina with described seed liquor, in 25 ~ 37 DEG C, shaking culture 2 ~ 12h under the condition of pH4.0 ~ 7.5, shaking speed 150 ~ 200r/min, obtain decolouring nutrient solution; The initial concentration of described Viola crystallina is 10 ~ 200mg/L, and described decolouring medium component is counted with g/L: peptone 8 ~ 12, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, and all the other compositions are water.
4. the application of rhodotorula mucilaginosa described in claim 1 in fermentative production carotenoid.
5. the application of rhodotorula mucilaginosa in fermentative production carotenoid according to claim 4, be is characterized in that being realized by following steps: described rhodotorula mucilaginosa is carried out slant culture and seed culture, obtains activated seed liquid; Described seed liquor is seeded to fermention medium by the inoculum size of 2% by volume, and in 25 ~ 32 DEG C, the condition bottom fermentation of pH5.0 ~ 7.0 cultivates 36 ~ 48h, obtains the fermented liquid containing carotenoid; Described fermentation medium components is counted with g/L: glucose 10 ~ 12, peptone 10 ~ 12, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, and all the other compositions are water.
6. the application of rhodotorula mucilaginosa in Viola crystallina degradation and decolorization according to claim 3, it is characterized in that: described slant culture temperature is 32 DEG C, incubation time 40h, slant culture based component counts peptone 10 ~ 12 with g/L, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, agar 12 ~ 14, all the other compositions are water, pH5.5 ~ 6.0.
7. the application of rhodotorula mucilaginosa in fermentative production carotenoid according to claim 5, it is characterized in that: described slant culture temperature is 32 DEG C, incubation time 40h, slant culture based component counts peptone 10 ~ 12 with g/L, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, agar 12 ~ 14, all the other compositions are water, pH5.5 ~ 6.0.
8. the application of rhodotorula mucilaginosa in fermentative production carotenoid according to claim 5, it is characterized in that: described seed culture temperature 32 DEG C, incubation time 20h, shaking speed 150r/min, seed culture based component counts peptone 10 ~ 12 with g/L, yeast extract 5 ~ 7, sodium-chlor 5 ~ 7, magnesium sulfate heptahydrate 5 ~ 7, all the other compositions are water, pH5.5 ~ 6.0.
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| CN103993062B (en) * | 2014-04-03 | 2016-08-31 | 河南师范大学 | Rhodotorula mucilaginosa is utilized to process the method that antibiotic fermentation waste water produces carotenoid |
| CN104178432B (en) * | 2014-08-08 | 2017-01-11 | 成都医学院 | Rhodotorula mucilaginosa and application thereof |
| CN105420128B (en) * | 2015-12-02 | 2018-09-25 | 湖北工业大学 | A kind of selenium-rich rhodotorula mucilaginosa bacterial strain FXY-7 and its cultural method and the application in shrimp feed additive |
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| CN103409328A (en) | 2013-11-27 |
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