CN103397069A - Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal - Google Patents
Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal Download PDFInfo
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Abstract
The invention discloses a method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal, which is characterized by comprising the following steps: (1) performing cartilage hydrolysis pretreatment; (2) performing enzymolysis; (3) performing centrifugal separation to obtain the bone oil and the bone meal; (4) concentrating; (5) adsorbing; (6) preparing the protein peptides; and (7) preparing the chondroitin sulfate. The invention adopts a composite enzyme hydrolysis technology and realizes synchronous hydrolysis of the chondroitin sulfate and the protein peptides. The chondroitin sulfate is released, and meanwhile, the protein peptides in the cartilage are hydrolyzed. A large amount of proteins and other pollutants in the traditional chondroitin sulfate production are recycled, thus realizing synchronous production of the chondroitin sulfate, the protein peptides, the bone oil and the bone meal, realizing comprehensive utilization of the cartilage, and simultaneously realizing energy saving and emission reduction and achieving remarkable economic benefits and social benefits.
Description
Technical field
The invention belongs to chemical technology field, be specifically related to a kind of chondroitin sulfate and clean the method for coproduction protein peptide, animal oil and bone meal.
Background technology
Chondroitin sulfate (Chondroitin Sulfate is called for short CS) is to be extracted a kind of important acidic mucopolysaccharide of preparation by cartilaginous tissues such as bone, tracheaes in animal larynx bone, nose.Chondroitin sulfate is distributed widely in the tissues such as people and some animals such as ox, sheep, pig, fish, and its main component is the mixture of the isomer such as chondroitin-4-suleate (CSA) and 6-chondroitin sulfate (CSC).Chondroitin sulfate is mainly used in treating cardiovascular and cerebrovascular disease, joint disease, and has reducing blood-fat, antithrombotic, the effect such as antitumor.China's chondroitin sulfate exports to the many countries of US and European in a large number, occupies the 3rd in the ten large class Chinese patent medicine raw materials that world market is badly in need of.In recent years, chondroitin sulfate is being prevented and treated coronary heart disease, sacroiliitis and the aspect such as antitumor is just day by day in widespread attention.
The method of present domestic chondroitin sulfate production mainly contains three kinds: salt solution, based on alkaline hydrolysis and enzymolysis process, the more method of domestic use is that the method that adopts diluted alkaline or concentrated base to combine with enzymolysis is extracted chondroitin sulfate, adopt this alkaline process enzymolysis production process to exist complex steps, cycle longer, generally need 3-5 days, quality product and yield are unstable, in high temperature steaming, alkaline extraction, enzymolysis and decolorization, chondroitin sulfate is subject to havoc, product yield is on the low side, solution colour is deepened, alcohol consumption quantity is large, and production cost is high.
And a large amount of animal proteinums that produce in present chondroitin sulfate production process and animal oil and bone slag and degraded product thereof all fail utilize and discharge, and the organic waste water that is untreated is pitch-black smelly, and eutrophication, and water surrounding is brought severe contamination.Only have at present the relatively large chondroitin sulfate of small part manufacturing enterprise to administer sewage, generally take simple physical chemistry to be treated to main end treatment mode more, be about to a large amount of protein pollutants and all concentrate on the afterbody processing, disposable input is larger, running expense is high, protein is not utilized, and waste discharge is difficult to realize up to standard.
China's chondroitin sulfate output accounts for more than 80% of Gross World Product, and the technique that the research chondroitin sulfate cleans coproduction protein peptide, animal oil and bone meal will have prior economy, society and Significance for Environment.
Summary of the invention
For solving problems of the prior art, the purpose of this invention is to provide a kind of method that chondroitin sulfate cleans coproduction protein peptide, animal oil and bone meal, the method prepares chondroitin sulfate by enzyme process, and production protein peptide simultaneously, animal oil and bone meal have been realized zero pollution, zero release.The technical solution adopted in the present invention is: a kind of chondroitin sulfate cleans the method for coproduction protein peptide, animal oil and bone meal, it is characterized in that, said method comprising the steps of:
(1) cartilage hydrolysis pre-treatment: animal cartilage is crushed to the following fragment of 1cm, puts into vacuum tank, add distilled water, control vacuum tightness 0.01-0.03MPa in tank, temperature 65-80 ℃, heating 2-2.5h;
(2) enzymolysis: preprocessing solution is got in enzymatic vessel and regulated pH to 7.5-8.0; Add the trypsinase of cartilage quality 0.3-0.5%, the flavor protease of 0.1-0.3% and the Sumizyme MP of 0.4-0.7%, enzymolysis;
(3) centrifugation obtains animal oil and bone meal: enzymolysis utilizes the dish piece type three-phase centrifuge centrifugation after finishing, and obtains respectively animal oil, filtrate and bone slag, obtains bone meal after the bone ground-slag is broken;
(4) concentrated: the filtrate after centrifugal concentrate by Three-effect concentration device, in concentration process, controls one and imitate temperature at 90-95 ℃, two imitate at 80-85 ℃, and triple effect is at 65-70 ℃, when being concentrated to volume and being the 50-60% of original filtrate, stop concentrating;
(5) absorption: concentrated solution is squeezed into resin container absorption, and in adsorption process, feed temperature is controlled at 65-70 ℃, pH 7.0-7.5, absorption 2-2.5h;
(6) protein peptide preparation: after adsorb, all filtrate is squeezed into concentration tank and is concentrated, and when solid substance, during in the 40-50% left and right, stops concentrating, and concentrated solution is squeezed into spray-drying tower carry out drying and obtain protein peptide;
(7) chondroitin sulfate preparation: be 1.12-1.13 with proportion, pH squeezes into resin container at the salt solution of 7.0-7.5, and salt solution is as the criterion higher than resin 3-5 centimetre, opening pressurized air stirs, after resolving 2-2.5h, finish to resolve, desorbed solution is squeezed into desorbed solution storage tank concentrate drying obtain chondroitin sulfate.
In above-mentioned steps (1), the amount of adding distil water is the 80-95% of cartilage quality.
In above-mentioned steps (2) in enzymolysis process constantly regulator solution pH remain on 7.5-8.0, maintenance temperature 35-40 ℃, enzymolysis 4-6h.
In above-mentioned steps (6), molecular weight accounts for more than 85% in the protein peptide below 5KD.
Beneficial effect of the present invention:
1, adopt the combinative enzyme hydrolysis technology, realize the same one-step hydrolysis of chondroitin sulfate and protein peptide.When chondroitin sulfate is discharged, the protein peptide in cartilage is hydrolyzed.
2, a large amount of protein and other pollutents traditional chondroitin sulfate produced are recycled, realized the synchronous production of chondroitin sulfate, protein peptide, animal oil and bone meal, realized the comprehensive utilization of cartilage, energy-saving and emission-reduction simultaneously, economic benefit and social benefit are remarkable.
Embodiment
The invention will be further described below in conjunction with specific embodiment, to help understanding content of the present invention.
Embodiment 1
Selected 1000 kilograms of chicken leg bones, be crushed to the following fragment of 1cm, puts into vacuum tank, adds 800 kilograms of distilled water, utilizes steam heating, and in the control tank, temperature is 65 ℃, vacuum tightness 0.01MPa, heating 2h.Feed liquid is got in enzymatic vessel and regulated pH to 7.5; Add 3 kilograms of trypsinase, flavor protease and 4 kilograms of Sumizyme MPs of 1 kilogram, keep 35 ℃ of temperature, enzymolysis 4h.Enzymolysis utilizes the dish piece type three-phase centrifuge centrifugation after finishing, and the upper strata separation obtains 50 kilograms of animal oil; Obtain 760 kilograms of bone meal after the bone ground-slag is broken.Filtrate after centrifugal is concentrated by Three-effect concentration device, in concentration process, control an effect temperature at 90 ℃, two effects are at 80 ℃, and triple effect is at 65 ℃, be concentrated to volume and be original filtrate 50% the time, stop concentrating.Concentrated solution is squeezed into resin container absorption, and in adsorption process, feed temperature is controlled at 65 ℃, and pH 7.0, absorption 2h.After absorption finishes, all filtrate after absorption is squeezed into concentration tank concentrated, when solid substance, 40% the time, stop concentrating, concentrated solution is squeezed into spray-drying tower carry out drying and obtain 160 kilograms of protein peptide, 140 kilograms of the protein peptide of molecular weight below 5KD.Be that 1.12, pH squeezes into resin container at 7.0 salt solution with proportion, salt solution, higher than 3 centimetres of resins, is opened pressurized air and is stirred, and after resolving 2h, finishes to resolve, and desorbed solution is squeezed into desorbed solution storage tank concentrate drying obtain 10.5 kilograms of chondroitin sulfates.
Embodiment 2
Selected 1000 kilograms of chicken ball bones, be crushed to the following fragment of 1cm, puts into vacuum tank, adds 900 kilograms of distilled water, utilizes steam heating, controls temperature 70 C in tank, vacuum tightness 0.02MPa, heating 2.3h.Feed liquid is got in enzymatic vessel and regulated pH to 8.0; Add 4 kilograms of trypsinase, flavor protease and 5 kilograms of Sumizyme MPs of 2 kilograms, keep 38 ℃ of temperature, enzymolysis 5h.Enzymolysis utilizes the dish piece type three-phase centrifuge centrifugation after finishing, and the upper strata separation obtains 55 kilograms of animal oil; Obtain 746 kilograms of bone meal after the bone ground-slag is broken.Filtrate after centrifugal is concentrated by Three-effect concentration device, in concentration process, control an effect temperature at 93 ℃, two effects are at 83 ℃, and triple effect is at 68 ℃, be concentrated to volume and be original filtrate 55% the time, stop concentrating.Concentrated solution is squeezed into resin container absorption, and in adsorption process, feed temperature is controlled at 68 ℃, and pH 7.5, absorption 2.3h.After absorption finishes, all filtrate after absorption is squeezed into concentration tank concentrated, when solid substance, 45% the time, stop concentrating, concentrated solution is squeezed into spray-drying tower carry out drying and obtain 155 kilograms of protein peptide, 138 kilograms of the protein peptide of molecular weight below 5KD.Be that 1.13, pH squeezes into resin container at 7.5 salt solution with proportion, salt solution, higher than 4 centimetres of resins, is opened pressurized air and is stirred, and after resolving 2.3h, finishes to resolve, and desorbed solution is squeezed into desorbed solution storage tank concentrate drying obtain 16.5 kilograms of chondroitin sulfates.
Embodiment 3
Selected 1000 kilograms of duck breastbones, be crushed to the following fragment of 1cm, puts into vacuum tank, adds 950 kilograms of distilled water, utilizes steam heating, and in the control tank, temperature is 80 ℃, vacuum tightness 0.03MPa, heating 2.5h.Feed liquid is got in enzymatic vessel and regulated pH to 8.0; Add 5 kilograms of trypsinase, flavor protease and 7 kilograms of Sumizyme MPs of 3 kilograms, keep 40 ℃ of temperature, enzymolysis 6h.Enzymolysis utilizes the dish piece type three-phase centrifuge centrifugation after finishing, and the upper strata separation obtains 72 kilograms of animal oil; Obtain 735 kilograms of bone meal after the bone ground-slag is broken.Filtrate after centrifugal is concentrated by Three-effect concentration device, in concentration process, control an effect temperature at 95 ℃, two effects are at 85 ℃, and triple effect is at 70 ℃, be concentrated to volume and be original filtrate 50% the time, stop concentrating.Concentrated solution is squeezed into resin container absorption, and in adsorption process, feed temperature is controlled at 70 ℃, and pH 7.5, absorption 2.5h.After absorption finishes, all filtrate after absorption is squeezed into concentration tank concentrated, when solid substance, 50% the time, stop concentrating, concentrated solution is squeezed into spray-drying tower carry out drying and obtain 175 kilograms of protein peptide, 162 kilograms of the protein peptide of molecular weight below 5KD.Be that 1.13, pH squeezes into resin container at 7.5 salt solution with proportion, salt solution, higher than 5 centimetres of resins, is opened pressurized air and is stirred, and after resolving 2.5h, finishes to resolve, and desorbed solution is squeezed into desorbed solution storage tank concentrate drying obtain 13.8 kilograms of chondroitin sulfates.
Can find out by above-described embodiment, the present invention has following characteristics:
1, adopt the combinative enzyme hydrolysis technology, realize the same one-step hydrolysis of chondroitin sulfate and protein peptide.When chondroitin sulfate is discharged, the protein peptide in cartilage is hydrolyzed.
2, a large amount of protein and other pollutents traditional chondroitin sulfate produced are recycled, realized the synchronous production of chondroitin sulfate, protein peptide, animal oil and bone meal, realized the comprehensive utilization of cartilage, energy-saving and emission-reduction simultaneously, economic benefit and social benefit are remarkable.
The above, be only the specific embodiment of the present invention, is not limited to this, anyly is familiar with those skilled in the art in the technical scope that this patent discloses, and can expect easily changing or replacing, within all should being encompassed in protection scope of the present invention.
Claims (4)
1. a chondroitin sulfate cleans the method for coproduction protein peptide, animal oil and bone meal, it is characterized in that, said method comprising the steps of:
(1) cartilage hydrolysis pre-treatment: animal cartilage is crushed to the following fragment of 1cm, puts into vacuum tank, add distilled water, control vacuum tightness 0.01-0.03MPa in tank, temperature 65-80 ℃, heating 2-2.5h;
(2) enzymolysis: preprocessing solution is got in enzymatic vessel and regulated pH to 7.5-8.0; Add the trypsinase of cartilage quality 0.3-0.5%, the flavor protease of 0.1-0.3% and the Sumizyme MP of 0.4-0.7%, enzymolysis;
(3) centrifugation obtains animal oil and bone meal: enzymolysis utilizes the dish piece type three-phase centrifuge centrifugation after finishing, and obtains respectively animal oil, filtrate and bone slag, obtains bone meal after the bone ground-slag is broken;
(4) concentrated: the filtrate after centrifugal concentrate by Three-effect concentration device, in concentration process, controls one and imitate temperature at 90-95 ℃, two imitate at 80-85 ℃, and triple effect is at 65-70 ℃, when being concentrated to volume and being the 50-60% of original filtrate, stop concentrating;
(5) absorption: concentrated solution is squeezed into resin container absorption, and in adsorption process, feed temperature is controlled at 65-70 ℃, pH 7.0-7.5, absorption 2-2.5h;
(6) protein peptide preparation: after adsorb, all filtrate is squeezed into concentration tank and is concentrated, and when solid substance, during in the 40-50% left and right, stops concentrating, and concentrated solution is squeezed into spray-drying tower carry out drying and obtain protein peptide;
(7) chondroitin sulfate preparation: be 1.12-1.13 with proportion, pH squeezes into resin container at the salt solution of 7.0-7.5, and salt solution is as the criterion higher than resin 3-5 centimetre, opening pressurized air stirs, after resolving 2-2.5h, finish to resolve, desorbed solution is squeezed into desorbed solution storage tank concentrate drying obtain chondroitin sulfate.
2. a kind of chondroitin sulfate according to claim 1 cleans the method for coproduction protein peptide, animal oil and bone meal, it is characterized in that, in described step (1), the amount of adding distil water is the 80-95% of cartilage quality.
3. a kind of chondroitin sulfate according to claim 1 cleans the method for coproduction protein peptide, animal oil and bone meal, it is characterized in that, in described step (2) in enzymolysis process constantly regulator solution pH remain on 7.5-8.0, maintenance temperature 35-40 ℃, enzymolysis 4-6h.
4. a kind of chondroitin sulfate according to claim 1 cleans the method for coproduction protein peptide, animal oil and bone meal, it is characterized in that, in described step (6), molecular weight accounts for more than 85% in the protein peptide below 5KD.
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Cited By (6)
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| CN104480168A (en) * | 2014-11-25 | 2015-04-01 | 荣成宏业海洋科技有限公司 | Method for preparing chondroitin sulfate and co-producing collagen peptide and biological calcium by using fishbone |
| CN104757260A (en) * | 2015-04-22 | 2015-07-08 | 如皋市永兴肠衣有限公司 | Process for preparing DPS (dried porcine solubles) from sausage casing waste residues |
| CN104757259A (en) * | 2015-04-22 | 2015-07-08 | 如皋市永兴肠衣有限公司 | Process for hydrolysis of intestinal contents by trypsin |
| CN104798988A (en) * | 2015-04-22 | 2015-07-29 | 如皋市永兴肠衣有限公司 | Process for preparing feed protein from heparin residue |
| CN111533826A (en) * | 2019-12-31 | 2020-08-14 | 临沂新程金锣肉制品集团有限公司 | Method for simultaneously extracting chondroitin sulfate and collagen peptide from pig bones |
| CN114246335A (en) * | 2022-01-19 | 2022-03-29 | 福建省深蓝生物科技有限公司 | Nutritional food rich in chondroitin sulfate and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104480168A (en) * | 2014-11-25 | 2015-04-01 | 荣成宏业海洋科技有限公司 | Method for preparing chondroitin sulfate and co-producing collagen peptide and biological calcium by using fishbone |
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| CN114246335A (en) * | 2022-01-19 | 2022-03-29 | 福建省深蓝生物科技有限公司 | Nutritional food rich in chondroitin sulfate and preparation method thereof |
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