CN103387969A - Complex plant hydrolytic enzyme used for extracting aromatic compound from aromatic raw materials - Google Patents
Complex plant hydrolytic enzyme used for extracting aromatic compound from aromatic raw materials Download PDFInfo
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Abstract
The invention discloses a complex plant hydrolytic enzyme used for extracting an aromatic compound from aromatic raw materials. The complex plant hydrolytic enzyme disclosed by the invention is composed of exo-beta-glucanase, endo-beta-glucanase, beta-glucosaccharase and rhizomucor mieheilipase by certain proportions. According to the complex plant hydrolytic enzyme, the aromatic raw materials can be degraded more completely, and the extraction separation efficiency and aromatic efficiency are obviously improved; therefore, the complex plant hydrolytic enzyme has a high market application value.
Description
Technical field
The present invention relates to extract the enzyme field in the course of processing, be specifically related to a kind of composite plant lytic enzyme used while causing aroma compounds that extracts from cause perfume material.
Background technology
Tradition enzyme engineering composite plant lytic enzyme used mostly is circumscribed beta-glucanase, Endo-β-glucanase, beta-glucosidase, the composite plant lytic enzyme that these three kinds of enzymes form can the degrading plant cell walls the chief component Mierocrystalline cellulose, the degrading plant cell walls discharges aroma component fast.But have some greases plant itself, can hinder the release of plant part aroma component, simultaneous grease produces the inconsistent material of some and the local flavor of plant own through peroxidation in storage for a long time, affects the fragrance of extracting substance.
Summary of the invention
The object of the present invention is to provide a kind of composite plant lytic enzyme used while causing aroma compounds that extracts from cause perfume material, this enzyme can extract by the faster and better aroma component that will cause in perfume material more completely.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A kind ofly extract composite plant lytic enzyme used while causing aroma compounds from cause perfume material, by circumscribed beta-glucanase, Endo-β-glucanase, beta-glucosidase and rhizomucor miehei lipase, formed; Wherein, the volume of the interpolation of described composite plant lytic enzyme is to cause 1.5 ‰ of perfume material quality.
The perfume material that causes of the present invention comprises that all are with fragrance or special odor, can be used for extracting aroma component, particularly the material of spices, essence etc., be particularly preferably offal, discarded tobacco leaf, agalloch eaglewood, red date, Salvia japonica Thunb., pale flag, sweet basil, sandalwood, Persea cubeba, lavandula angustifolia or Hyssopus officinalis.
Of the present invention to extract the method cause aroma compounds from cause perfume material can be traditional enzyme engineering method, can be also the improved all kinds of aroma compounds extracting method that causes, and particularly preferred method comprises the steps:
(1) get 4kg and cause perfume material, dry 10～14h in 50～80 ℃ of baking ovens, pulverize and pass through 60～80 mesh sieves and filter, add pure water, making solid-liquid ratio is 1:3～6, is placed in the ultrasonication tank, at ultrasound intensity, is 2.5～4.5W/cm
2Under, broken 1.5h;
(2) get citric acid 5～30g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 5.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, stir, add retort reaction 4～8h, temperature of reaction is 45～55 ℃, and the pH value is 4～8;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 45～50 ℃, and rotating speed is 50～80rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds;
Wherein, described in step (3), the composite plant lytic enzyme is comprised of circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 2.5ml and rhizomucor miehei lipase 0.5ml.
Compared with prior art, the present invention has following beneficial effect:
The present invention adds rhizomucor miehei lipase on the circumscribed beta-glucanase of original plant extract lytic enzyme, Endo-β-glucanase, beta-glucosidase, adding like this is to have utilized rhizomucor miehei lipase to degrade to be unfavorable for that the plant aroma component discharges and the characteristic of the grease of typical flavor; So both can the fast degradation plant cell wall, can also degrade and be unfavorable for that the plant aroma component discharges the grease with typical flavor; And,, by the mixing ratio of the present invention for various enzymes, can make that the resulting fragrance that causes fragrant extract is more pure, to extract the aroma component that obtains more.
Embodiment
Further explain the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.
In following examples, the reagent of employing and material unless stated otherwise, otherwise are all commercial general reagent and material; The experimental technique that adopts, unless stated otherwise, otherwise be all the ordinary method of the art.
Embodiment 1 offal
Method one: (1) gets the 4kg offal, dries 12h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is
3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.8, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL of the present invention after mixing, stir, add retort reaction 4h, temperature of reaction is 48 ℃, and the pH value is 7;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take offal as raw material cause aroma compounds GC-MS analytical results:
Table 1: that take offal as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g offal after converting)
Embodiment 2 discarded tobacco leafs
Method one: (1) gets the 4kg discarded tobacco leaf, dries 12h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 48 ℃, and the pH value is 7.2;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take discarded tobacco leaf as raw material cause aroma compounds GC-MS analytical results:
Table 2: that take discarded tobacco leaf as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g discarded tobacco leaf after converting)
Embodiment 3 agalloch eaglewood
Method one: (1) gets the 4kg agalloch eaglewood, dries 4h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 50 ℃, and the pH value is 6.5;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take agalloch eaglewood as raw material cause aroma compounds GC-MS analytical results:
Table 3: that take agalloch eaglewood as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g agalloch eaglewood of converting)
Embodiment 4 red dates
Method one: (1) gets the 4kg red date, dries 24h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 7.6;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take red date as raw material cause aroma compounds GC-MS analytical results:
Table 4: that take red date as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g red date that converts)
Embodiment 5 Salvia japonica Thunb.s
Method one: (1) gets the 4kg Salvia japonica Thunb., dries 18h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 6.2;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take Salvia japonica Thunb. as raw material cause aroma compounds GC-MS analytical results:
Table 5: that take Salvia japonica Thunb. as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g Salvia japonica Thunb. of converting)
Embodiment 6 pale flags
Method one: (1) gets the 4kg pale flag, dries 18h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 6.1;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take pale flag as raw material cause aroma compounds GC-MS analytical results:
Table 6: that take pale flag as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g pale flag that converts)
Embodiment 7 sweet basils
Method one: (1) gets the 4kg sweet basil, dries 18h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 6.5;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take sweet basil as raw material cause aroma compounds GC-MS analytical results:
Table 7: that take sweet basil as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g sweet basil that converts)
Embodiment 8 sandalwoods
Method one: (1) gets the 4kg sandalwood, dries 4h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 7.3;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take sandalwood as raw material cause aroma compounds GC-MS analytical results:
Table 8: that take sandalwood as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g sandalwood that converts)
Embodiment 9 Persea cubeba
Method one: (1) gets the 4kg Persea cubeba, dries 12h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 5.5;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take Persea cubeba as raw material cause aroma compounds GC-MS analytical results:
Table 9: that take Persea cubeba as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g Persea cubeba of converting)
Embodiment 10 lavandula angustifolias
Method one: (1) gets the 4kg lavandula angustifolia, dries 18h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) get the 200mL primary extract and mix with the 2L pure water, add composite plant lytic enzyme 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 6.5;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take lavandula angustifolia as raw material cause aroma compounds GC-MS analytical results:
Table 10: that take lavandula angustifolia as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g lavandula angustifolia of converting)
Embodiment 11 Hyssopus officinalis
Method one: (1) gets the 4kg Hyssopus officinalis, dries 18h in 60 ℃ of baking ovens, pulverizes and passes through 60 mesh sieves and filter, and adds pure water 16L, and making solid-liquid ratio is 1:4, is placed in the ultrasonication tank, at ultrasound intensity, is 3W/cm
2Under, broken 1.5h;
(2) get citric acid 20g, add 100mL water, after dissolving, constant volume, to 200mL, obtains citric acid solution; Get citric acid solution 100mL, add after water 400mL stirs and be added to retort, survey the pH value after stirring 2min, regulating the pH value is 4.5, obtains primary extract;
(3) getting the 200mL primary extract mixes with the 2L pure water, add composite plant lytic enzyme (circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 2.5ml, rhizomucor miehei lipase 0.5ml) 6mL after mixing, add retort reaction 4h, temperature of reaction is 45 ℃, and the pH value is 6.2;
(4) after temperature reaction tank temperature to 80 ℃, keep 10min, open the condensation water for cooling;
(5) liquid in retort is taken out rear the filtration, first with whizzer, separate, then use the yarn bag filtration,, by Plate Filtration, obtain filtrate finally;
(6) filtrate is weighed, it is concentrated that equivalent joins the rotary evaporation bottle, and temperature is 50 ℃, and rotating speed is 60rpm, and evacuated pressure is less than 0.1Mpa; When residual solution is 2kg, stop evaporation, obtain causing aroma compounds.
Method two: step, with method one, only changes the composite plant lytic enzyme into: circumscribed beta-glucanase 1.5ml, Endo-β-glucanase 1.5ml, beta-glucosidase 3ml.
Be below extract take Hyssopus officinalis as raw material cause aroma compounds GC-MS analytical results:
Table 11: that take Hyssopus officinalis as raw material, extracts causes aroma compounds GC-MS analytical results
(μ g/g: contain the aroma component amount in every g Hyssopus officinalis that converts)
Claims (1)
1. one kind is extracted composite plant lytic enzyme used while causing aroma compounds from cause perfume material, it is characterized in that described composite plant lytic enzyme is comprised of circumscribed beta-glucanase, Endo-β-glucanase, beta-glucosidase and rhizomucor miehei lipase;
Wherein, the volume of the interpolation of described composite plant lytic enzyme is to cause 1.5 ‰ of perfume material quality.
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| CN103642595A (en) * | 2013-12-02 | 2014-03-19 | 无锡合众信息科技有限公司 | Device for extracting aroma compound from lavender |
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| CN102638999A (en) * | 2010-03-05 | 2012-08-15 | 长谷川香料株式会社 | Method for producing tea extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103642595A (en) * | 2013-12-02 | 2014-03-19 | 无锡合众信息科技有限公司 | Device for extracting aroma compound from lavender |
| CN103602522A (en) * | 2013-12-04 | 2014-02-26 | 中山鼎晟生物科技有限公司 | Extraction method for plant aromatic stock solution and preparation method for solidified essence from aromatic stock solution |
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Application publication date: 20131113 |