CN103381208B - The application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament - Google Patents
The application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament Download PDFInfo
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- CN103381208B CN103381208B CN201310321337.8A CN201310321337A CN103381208B CN 103381208 B CN103381208 B CN 103381208B CN 201310321337 A CN201310321337 A CN 201310321337A CN 103381208 B CN103381208 B CN 103381208B
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Abstract
The present invention proposes the application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament.The Sorbus sibirica fruit extract that the present invention obtains, shown by the research of extract to stomach cancer cell BGC823 suppression ratio: Sorbus sibirica fruit extract has the ability of very strong suppression Growth of Gastric, when extract concentrations is 200 μ g/mL, when cultivating 48h, suppression ratio just reaches 50%.Therefore, the anti-stomach cancer cell ability of Sorbus sibirica fruit extract is strong, is a good selection of the efficient natural anti-gastric cancer medicament of development of new or health product.
Description
Technical field
The invention belongs to the purposes field of medicine, relate to Sorbus sibirica fruit extract particularly and preparing the application in the medicine or health product with anti-stomach cancer cell effect.
Background technology
Cancer is the disease that developed country's mortality rate is the highest, also becomes to be only second to the second largest fatal disease of cardiovascular disease in developing country gradually.Compared with other cancer, gastric cancer is a class complexity, multifactorial disease, and the gastric cancer of more than 90% all belongs to adenocarcinoma of stomach, and other to be lymphoma or stroma be main sarcoma, still has very high mortality rate in the world.Natural product is the important sources of drug development, and how natural product being applied to cancer drug exploitation and clinical treatment aspect, is the problem that those skilled in the art constantly explores.
Sorbus sibirica is Rosaceae (Rosaceae) Fructus Sorbi Pohuashanensis, and belong to dungarunga, be only distributed in upstream, Burqin river in China, under liking being grown on PiceameyeriRehd. Et Wils. and fir mixed forest, its tree-like grace, figure hold directly, and leaf is large-scale winglike compound leaf, is rich in ornamental value.Spring, snow-white flower completely set fragrance, and the scarlet wish of mellow fruit in autumn is dripped.
Current Sorbus sibirica fruit extract does not also appear in the newspapers in the application aspect of anticancer.
Summary of the invention
The present invention is for the purpose of the exploitation seeking new and effective suppression stomach cancer cell medicine or health product, proposes Sorbus sibirica fruit extract and is preparing the application in the medicine or health product with anti-stomach cancer cell effect.
The Sorbus sibirica fruit extract that the present invention obtains, shown by the research of extract to stomach cancer cell BGC823 suppression ratio: Sorbus sibirica fruit extract has the ability of very strong suppression Growth of Gastric, when extract concentrations is 200 μ g/mL, when cultivating 48h, suppression ratio just reaches 50%.Therefore, the anti-stomach cancer cell ability of Sorbus sibirica fruit extract is strong, is a good selection of the efficient natural anti-gastric cancer medicament of development of new or health product.
Accompanying drawing explanation
Fig. 1 Sorbus sibirica fruit extract is to the inhibitory action of stomach cancer cell BGC823.
Detailed description of the invention
Embodiment 1
(1) preparation of Sorbus sibirica fruit extract: take Sorbus sibirica fruit 5 g, ethanol with 60% soaks 1 h at 60-70 DEG C, put into the ultrasonic 30min of ultrasonic cleaner, continuous 2 times, use Rotary Evaporators concentrated extracting solution again, obtain Sorbus sibirica fruit extract dry powder through vacuum lyophilization.
(2) effect test
1, sample preparation
The preparation of 1640 complete mediums: hyclone puts into 55 DEG C of water-bath 30min deactivation, by volume percentage ratio, get the hyclone of 10% and the RPMI-1640 mix homogeneously of 90%, then add dual anti-(penicillin and streptomycin are by weight 1:1 mixing), addition is 100u/ml, mix homogeneously, makes 1640 complete mediums.
The Sorbus sibirica fruit extract dry powder 0.01g obtained is dissolved in the alcoholic solution 1ml of 20%, filtration sterilization, being mixed with concentration with 1640 complete mediums is mother solution containing Sorbus sibirica fruit extract 1mg/mL, is stored in-4 DEG C of Refrigerator stores.
Get the mother solution containing Sorbus sibirica fruit extract 1mg/mL, with 1640 complete mediums be mixed with respectively concentration be 60 μ g/mL, 80 μ g/mL, 100 μ g/mL, 150 μ g/mL, 200 μ g/mL, 250 μ g/mL extract solution for subsequent use.For following effect experimental.
2, test
The stomach cancer cell BGC823(cell density of trophophase of taking the logarithm is about 3 × 10
7 ) being inoculated in 96 orifice plates, 1000 cells are inoculated in every hole; Be divided into following seven groups:
Matched group: 1640 complete mediums of 100 μ L;
Experimental group: extract solution for subsequent use (60 μ g/mL, 80 μ g/mL, 100 μ g/mL, 150 μ g/mL, 200 μ g/mL, 250 μ g/mL) adds 100 μ L respectively, often organizes 6 parallel holes;
Cultivating in 37 DEG C of incubators, respectively at adding 5mg/mLMTT solution 10 μ L after 24h, 48h, 72h in each hole, continuing to cultivate after 4h, every hole add 100 μ LDMSO dissolving crystallized after, spend the night in 37 DEG C of incubators; Measure the absorbance of each hole at 490nm place with enzyme-linked immunosorbent assay instrument, be calculated as follows cell inhibitory rate, result as shown in Figure 1.
Suppression ratio (%)=(1 one A
experiment/ A
contrast) X100%
As shown in Figure 1, Sorbus sibirica fruit extract has the ability of very strong suppression Growth of Gastric, and when extract concentrations is 100 μ g/mL, when cultivating 48h, suppression ratio just reaches 42%; When extract concentrations is 200 μ g/mL, when cultivating 48h, suppression ratio just reaches 50%.Therefore, the anti-stomach cancer cell ability of Sorbus sibirica fruit extract is strong, is a good selection of the efficient natural anti-gastric cancer medicament of development of new or health product.
Claims (1)
1. the application of Sorbus sibirica fruit extract in preparation treatment stomach cancer cell BGC823 medicine, the preparation method of described Sorbus sibirica fruit extract: get Sorbus sibirica fruit, ethanol with 60% soaks 1 h at 60-70 DEG C, put into the ultrasonic 30min of ultrasonic cleaner, then use Rotary Evaporators concentrated extracting solution; Sorbus sibirica fruit extract concentration is 200 μ g/mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310321337.8A CN103381208B (en) | 2013-07-29 | 2013-07-29 | The application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament |
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| CN201310321337.8A CN103381208B (en) | 2013-07-29 | 2013-07-29 | The application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament |
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| Publication Number | Publication Date |
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| CN103381208A CN103381208A (en) | 2013-11-06 |
| CN103381208B true CN103381208B (en) | 2015-08-12 |
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| CN201310321337.8A Active CN103381208B (en) | 2013-07-29 | 2013-07-29 | The application of Sorbus sibirica fruit extract in preparation treatment gastric cancer medicament |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2578457C1 (en) * | 2015-02-04 | 2016-03-27 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт фармакологии и регенеративной медицины имени Е.Д. Гольдберга" | Agent with antitumor and antimetastatic activity, increasing antimetastatic effect of cyclophosphan |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481643A (en) * | 2008-12-25 | 2009-07-15 | 辽宁省干旱地区造林研究所 | Aroniamelanocarpa fruit wine and brewing method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481643A (en) * | 2008-12-25 | 2009-07-15 | 辽宁省干旱地区造林研究所 | Aroniamelanocarpa fruit wine and brewing method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 5 种野生浆果的抗氧化和抗细胞增殖活性;樊梓鸾等;《食品科学》;20101231;第31卷(第17期);第148-152页 * |
| Aikaterini Termentzi,etal..Antioxidant activities of various extracts and fractions of Sorbus domestica fruits at different maturity stages.《Food Chemistry》.2006,(第98期),599–608. * |
| ANNE T. HUKKANEN,etal..Antioxidant Capacity and Phenolic Content of Sweet Rowanberries.《J. Agric. Food Chem》.2006,112-119. * |
| 超声波辅助提取技术研究进展;廖维良等;《广东药学院学报》;20120630;第28卷(第3期);第347-350页 * |
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