CN103344757A - Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof - Google Patents
Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof Download PDFInfo
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- CN103344757A CN103344757A CN2013103177155A CN201310317715A CN103344757A CN 103344757 A CN103344757 A CN 103344757A CN 2013103177155 A CN2013103177155 A CN 2013103177155A CN 201310317715 A CN201310317715 A CN 201310317715A CN 103344757 A CN103344757 A CN 103344757A
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Abstract
The invention discloses a piece of horizontal test paper for foot-and-mouth disease antibodies, which comprises an oscillator, a test box and horizontal test paper for foot-and-mouth disease antibodies, wherein the horizontal test paper for foot-and-mouth disease antibodies is arranged in the test box, the side wall of the bottom of the test box is provided with a test paper inlet, and the shape of the test paper inlet fits with that of the horizontal test paper for foot-and-mouth disease antibodies, so that the horizontal test paper for foot-and-mouth disease antibodies is inserted in the test box from the test paper inlet; the horizontal test paper for foot-and-mouth disease antibodies is provided with a test area; the top of the oscillator is provided with a test fluid inlet, the bottom of the oscillator is provided with a test fluid outlet, and the test fluid outlet is provided with an openable-closeable valve; the test fluid outlet of the oscillator is communicated with an opening at the top of the test box, and the position of the opening at the top of the test box fits with the position of the test area of the horizontal test paper for foot-and-mouth disease antibodies; the test area of the horizontal test paper for foot-and-mouth disease antibodies comprises an absorption pad arranged on a baseplate, and the absorption pad is made of porous fibers; one end of the absorption pad is sequentially provided with an immune-gold localization layer, a nitrocellulose membrane and an absorption layer.
Description
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Technical field
The present invention relates to field of medical article technology, especially relate to a kind of foot-and-mouth disease antibody level detection test paper and preparation method.
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Background technology
Aftosa (Foot-and-Mouth, FMD) be by foot and mouth disease virus cause a kind of acute, hot, the height contagiousness animal epidemic, it mainly infects liking artiodactyls such as pig, ox, sheep, in case take place to be difficult to control, can cause significant impact to social economy.
In view of tremendous economic loss and social influence that aftosa can cause, national governments all pay much attention to the study on prevention of aftosa.Now the antibody detection method of Cai Yonging mainly contains CFT, VNT, agglutination test, immunity diffusion and ELISA etc., but these methods all need detect in the laboratory, and sense cycle is long, and row detection fast and accurately can't the counterpart hoof sprints.
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Summary of the invention
Detect the problem that need finish at current aftosa in the laboratory, embodiment of the invention proposition is a kind of can on-the-spotly to detect to improve foot-and-mouth disease antibody level detection test paper and the preparation method who detects real-time to the aftosa antibody horizontal.
In order to realize this purpose, the invention provides a kind of foot-and-mouth disease antibody level detection test paper, comprise oscillator, detect box and be arranged at the interior foot-and-mouth disease antibody level detection test paper of described detection box; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively; Described detection box bottom sidewall is provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper inserts in the described detection box from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box top and the detection zone of described foot-and-mouth disease antibody level detection test paper is suitable.
Wherein, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
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Simultaneously, the embodiment of the invention has also proposed the preparation method of aforementioned any one foot-and-mouth disease antibody level detection test paper, comprising:
The colloidal gold solution of step 1,15～50 nanometers
The invention has the beneficial effects as follows: the present invention proposes a kind of many foot-and-mouth disease antibody level detection test papers and preparation method, can detect the aftosa antibody horizontal fast.Sample to be detected can be put into oscillator in use, rock this oscillator with abundant mixing after closing oscillator behind the adding dilution, the valve of opening the oscillator bottom then is instilled into mixed liquid on the detection zone of test paper accurately by this detection box.This structure can be used easily in the open air, improves the efficient that detects.
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Description of drawings
By the description of a preferred embodiment of the present invention being carried out below in conjunction with accompanying drawing, it is clearer that technical scheme of the present invention and technique effect thereof will become, and easy to understand more.Wherein:
Fig. 1 is the sectional structure synoptic diagram of the foot-and-mouth disease antibody level detection test paper of the embodiment of the invention.
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Embodiment
Below with reference to appended accompanying drawing a preferred embodiment of the present invention is described.
The invention provides a kind of foot-and-mouth disease antibody level detection test paper, its structure as shown in Figure 1, comprise oscillator 1, detect box 2 and be arranged at foot-and-mouth disease antibody level detection test paper 3 in the described detection box 2, described detection box bottom 2 sidewalls are provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper 3 of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper 3 inserts in the described detection box 2 from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve 11 of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box 2 tops and the detection zone of described foot-and-mouth disease antibody level detection test paper 3 is suitable; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively.
Wherein, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
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Simultaneously, the embodiment of the invention has also proposed the preparation method of aforementioned any one foot-and-mouth disease antibody level detection test paper, comprising:
The colloidal gold solution of step 1,15～50 nanometers
For the person of ordinary skill in the field, along with the development of technology, the present invention's design can realize by different way.Embodiments of the present invention are not limited in embodiment described above, and can change within the scope of the claims.
Claims (3)
1. a foot-and-mouth disease antibody level detection test paper is characterized in that, comprises oscillator, detects box and is arranged at the interior foot-and-mouth disease antibody level detection test paper of described detection box; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively; Described detection box bottom sidewall is provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper inserts in the described detection box from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box top and the detection zone of described foot-and-mouth disease antibody level detection test paper is suitable.
2. foot-and-mouth disease antibody level detection test paper according to claim 1, it is characterized in that, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
3. the preparation method of a foot-and-mouth disease antibody level detection test paper as claimed in claim 1 or 2 comprises:
The colloidal gold solution of step 1,15～50 nanometers
Step 2, pH value with colloidal gold solution transfers to 8.0～8.4 earlier, add 0.1～0.4mg foot and mouth disease virus by every 100ml colloidal gold solution therein again, after stirring, in solution, add animal blood serum albumen by 0.2～1g/100ml again, 4 ℃ left standstill 2～4 hours, again with the centrifugal removal sediment of above-mentioned colloidal gold solution, get supernatant, supernatant is got sediment through centrifugal treating again, the gained sediment is dissolved in the viral solution that obtains colloid gold label in the damping fluid of 0.02M pH7.4 Tris-Hcl of the animal blood serum albumen that contains weight ratio 0.2～0.6% and 0.01～0.06% Sodium azide by 4～10ml/100ml, till colloidal gold solution immersed glass fibre to liquid and begin to ooze out, form golden labeling antibody layer, golden labeling antibody layer is carried out drying handle;
Step 3, at nitrocellulose filter stage casing spray detection line, getting the anti-or mouse resisting O-type foot and mouth disease IgG of rabbit again, to transfer concentration be 1.5～2.5mg/ml, in the cellulose membrane stage casing, apart from detection line 0.5～1cm place, spray nature controlling line with Membrane jetter, spray film amount arranges by 10～15ul/1cm, the dry processing then, the phosphate buffer that contains calf serum with 0.01m PH7.0 carries out the drying processing again and obtains nitrocellulose filter 37 ℃ of sealings 30 minutes down after the PBS rinsing of 0.01ml pH7.0 again;
Step 3, golden labeling antibody layer, detection segment and absorbent pad material and absorber portion are fixed on adopt on the substrate that impermeable material makes successively.
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| CN2013103177155A CN103344757A (en) | 2013-07-25 | 2013-07-25 | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof |
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| CN2013103177155A CN103344757A (en) | 2013-07-25 | 2013-07-25 | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645682A (en) * | 2016-11-30 | 2017-05-10 | 中国农业科学院兰州兽医研究所 | Colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and preparation method of colloidal gold test paper |
| CN106770228A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | One kind is used for organophosphorus pesticide detection kit |
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| CN106770228A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | One kind is used for organophosphorus pesticide detection kit |
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Application publication date: 20131009 |