CN103344734A - Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography - Google Patents
Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography Download PDFInfo
- Publication number
- CN103344734A CN103344734A CN2013102871943A CN201310287194A CN103344734A CN 103344734 A CN103344734 A CN 103344734A CN 2013102871943 A CN2013102871943 A CN 2013102871943A CN 201310287194 A CN201310287194 A CN 201310287194A CN 103344734 A CN103344734 A CN 103344734A
- Authority
- CN
- China
- Prior art keywords
- eugenol
- solution
- sample
- standard
- gas chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography. The method comprises the following steps of: preparing a standard working solution and a sample solution, calculating the analysis and measurement result of gas chromatography-mass spectrography and the like. An internal standard method is used for quantifying, the optimized detection method is simple and accurate in operation, errors brought by sample pretreatment, instrument precision and the like are effectively reduced, the chromatographic peaks of a target object and an internal standard substance are well separated due to the adopted chromatographic conditions, the standard curve has high linear dependence in a concentration range of 0.5565-55.65mu g/mL, the correlation coefficient R2 is 0.9994, the detection limit is 1.15mg/kg, the average relative standard deviation is 3.15 percent, and the adding standard recovery is between 81.29 percent and 86.95 percent. Therefore, the method is high in sensitivity, high in repeatability and high in recovery rate and is suitable for qualitative determination and quantitative analysis of eugenol in edible flavor and fragrance.
Description
Technical field
The invention belongs to food additives physical and chemical index detection technique field, be specifically related to a kind of method of measuring eugenol in the flavoring essence spices based on gas chromatography-mass spectrography.
Background technology
The eugenol formal name used at school is 4-allyl-2-metoxyphenol, and English Eugenol by name claims eugenol again, Eugenol, clovenic acid etc.Molecular formula is C
10H
12O
2, molecular weight be 164.20, CAS number be 97-53-0.Pure eugenol is colourless liquid, is generally little yellow to yellow liquid, turns brown in air.Strong cloves fragrance and gentle hot fragrance are arranged, and substandard products then have the smell of burning.Boiling point is 253～254 ℃, and relative density is 1.064～1.068, and refractive index is 1.5400～1.5420(20 ℃).Atomic water-soluble, be dissolved in ethanol, ether, chloroform and essential oil.Natural eugenol mainly uses the caryophyllus oil, cinnamon leaves oil, cassia oil, camphor oil, the nutmeg wet goods that are rich in eugenol single from getting by alkali treatment; The eugenol of synthetic method preparation is raw material with guaiacol, adopts direct allylation such as allyl chloride, allyl alcohol and gets.
Eugenol has strong China pink muskiness, is that health is that Qing is the blending basis of essence, in the blending of essence such as making up, soap is used, eat use is arranged all.Be widely used in odor types such as fragrant common vetch, can be used as dressing agent and fixastive.Can be used for many floral perfumes such as rose etc.Also can be used in Xin Xiang, the banksia rose and east type, the perfume type, in essence such as the spicy that also can be used for eating, peppermint, nut, various fruital, Chinese date perfume (or spice) and the tobacco essence; In addition, eugenol has very strong sterilizing power, can be used for carious tooth as Bangesic, and has local antisepsis concurrently.Eugenol is the intermediate of some other spices, and derivant has isoeugenol, methyl eugenol, methylisoeugenol, aceteugenol, benzyl isoeugenol etc.
Use hygienic standard " GB2760-2011 " to stipulate according to food additives, eugenol is the flavorant that China's regulation permission is used, and record belongs to the food that allows use natural equivalent spices list catalogue, is encoded to I1101, and FEMA number is 2467.Mainly be used to prepare peppermint, nut, spicy food flavor and flavouring essence for tobacco, consumption needs by ordinary production, according to the requirement of limiting the quantity of that FEMA provides, use amount is 40～2000mg/kg in meat, 500mg/kg in the chewing gum, 9.6～100mg/kg in the flavouring, 33mg/kg in the baked goods, 32mg/kg in the candy, 3.1mg/kg in the cold drink, 1.4mg/kg in the soft drink, 0.6mg/kg in the cloth class D.
1985, eugenol was classified as doubtful carcinogenic substance by the IARC of IARC.According to the MSDS data information of eugenol, its LD50 is 1930～2680mg/kg (rat, per os), and ADI is 0～2.5 (FAO/WHO, 1994).This material toxicity on inhalation is 250 times that eat, and toxicity grading is for poisoning.This material is under a cloud to be caused skin or sensory system toxicity and causes immunotoxicity, and the source is legislation and the research document of comprehensive China, Canada, mechanism of the United States Federal, California, USA, Japan and European Union.Being classified as restriction by international fragrance essence association criterion uses.Literature research shows, material is even more serious to suck that eugenol is rarer than α-golden hop to the injury of lung tissue, ethyl eugenol and β-China pink are rare etc., and excessive suction easily causes pulmonary emphysema (selecting from document Food and Chemical Toxicology 45 (2007) 1948-1953).
Flavoring essence spices is important component part indispensable in the food production, and its security is the very important aspect of food overall security, and the source of foodsafety control is raw materials for production at all.Literature survey shows that the method for existing detection eugenol mainly contains gas chromatography and liquid chromatography, and the object of analysis mainly concentrates on the medicine aspect, and its matrix is different from edible flavoring essence spices.In view of eugenol often is used in essence and flavoring agent, therefore explore a kind of detection method of eugenol fast and accurately, the eugenol in the flavoring essence spices is control effectively, ensure the safety in utilization of edible additive, be very urgent and necessary.
Summary of the invention
Goal of the invention: at the deficiencies in the prior art, the purpose of this invention is to provide a kind of method of measuring eugenol in the flavoring essence spices based on gas chromatography-mass spectrography, fast, accurately detecting in the flavoring essence spices content of eugenol, measurement result accurately, measure disturb few.
Technical scheme: in order to realize the foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of method based on eugenol in the gas chromatography-mass spectrography mensuration flavoring essence spices comprises the steps:
(1) is internal standard compound with the anethole, makes solvent with ethanol, the preparation inner mark solution;
(2) be reference material with the eugenol, make solvent with ethanol, add inner mark solution respectively, be mixed with series standard solution through stepwise dilution;
(3) accurately take by weighing quantitative flavoring essence spices sample in centrifuge tube, add water and ethanol mixed solvent, vortex mixed scatter sample substrate, adds ethanol again, carries out ultrasonic extraction then; Then, add anhydrous CaCl
2, leave standstill 0.5h, place on the hydro-extractor centrifugally, get supernatant liquor and cross the organic phase filter membrane, get sample solution; Do not add sample, handle according to the method described above, get blank solution;
(4) with gas chromatograph-mass spectrometer (GCMS) standard operation solution, sample solution and blank solution are detected analysis respectively; Chromatographiccondition is: adopt DB-5MS(30m * 0.25mm * 0.25 μ m) capillary chromatographic column, carrier gas is helium, sample size is 1 μ L, injector temperature is 260 ℃, adopt the split sampling mode, split ratio is 10:1, and heating schedule is that initial temperature is 60 ℃, speed with 2 ℃/min is increased to 200 ℃, keeps 10min; Its mass spectrophotometry condition is 280 ℃ for the transmission line temperature, the ionization mode is EI, ion source temperature is 230 ℃, the quadrupole rod temperature is 150 ℃, scan mode is selectivity ion scan pattern (SIM), wherein to select ion be 164 to eugenol, and it is 148 that interior mark is selected ion, is 50ms to the monitoring time of each ion;
(5) being horizontal ordinate with eugenol in the standard operation solution and interior target concentration ratio, is ordinate with eugenol in the chromatogram and interior target peak area, carries out regretional analysis, obtains typical curve; With eugenol in the sample solution that records under the same terms and interior target chromatographic peak area, the substitution working curve converts according to following computing formula and to try to achieve the content of eugenol in the sample;
Computing formula is:
In the formula: X is the content of eugenol in the sample, and unit is mg/kg; C is the concentration by eugenol in the sample solution that reads on the standard working curve, and unit is mg/L; C0 is the concentration by eugenol in the blank solution that reads on the standard working curve, and unit is mg/L; V is the volume of extraction system, and unit is mL; M is the quality of sample, and unit is g.
In the step (1), the concentration of inner mark solution is 1000～2000mg/L.
In the step (2), accurately take by weighing 0.1～0.2g reference material eugenol, disperse, dissolve with 2mL methyl alcohol, be settled in the volumetric flask of 100mL with ethanol again, obtain the standard reserving solution that concentration is 1000～2000mg/L; Accurately pipette 50 μ L, 100 μ L, 500 μ L, 1mL and 5mL standard reserving solution respectively, add 100 μ L inner mark solutions more respectively, be settled to as solvent in the volumetric flask of 100mL with ethanol, obtain series standard solution.
In the step (5), the regression equation of the eugenol that standard working curve is corresponding is Y=0.901X-0.641, and related coefficient is 0.9994, and the range of linearity is 0.5565～55.65 μ g/mL, detects to be limited to 1.15mg/kg.
Beneficial effect: compared with prior art, employing of the present invention has following remarkable advantage based on the method for eugenol in the gas chromatography-mass spectrography mensuration flavoring essence spices:
(1) for the mensuration of eugenol, prior art is many with the external standard standard measure, and for the such complicated background system of flavoring essence spices, the quantitative measurement of object has interference unavoidably.And the inventive method is utilized inner mark method ration, can be without constant volume, and can reduce the error of being brought by pre-treating method reappearance and instrument precision problem;
(2) to have selected peak shape and the best DB-5MS post of separating effect be separating column to the inventive method, and adopt the GC/MS-SIM that can provide qualitative and quantitative information simultaneously as detecting pattern, improved the sensitivity of response signal, guaranteed the accuracy that detects;
(3) chromatographic condition that adopts of the inventive method makes the chromatographic peak of object and internal standard compound all separate better, and has the better linearity correlativity, detects and is limited to 1.15mg/kg;
(4) the average relative standard deviation of the inventive method is 1.97%, and recovery of standard addition illustrates the good reproducibility of this method between 81.29%～86.95%, recovery height.
Description of drawings
Fig. 1 is the chromatogram of standard operation solution;
Fig. 2 is the chromatogram of typical sample;
Fig. 3 is the chromatogram of blank solution.
Embodiment
Below in conjunction with specific embodiment the present invention is done further explanation.
Embodiment 1
(1) configuration of inner mark solution
Accurately take by weighing 0.1123g internal standard compound anethole, be settled in the volumetric flask of 100mL as solvent with ethanol, obtain the inner mark solution that concentration is 1123mg/L.Internal standard solution seals in 4 ℃ refrigerator and deposits, and is valid for three months;
(2) configuration of working stamndard solution
Accurately take by weighing 0.1113g reference material eugenol, be settled in the volumetric flask of 100mL as solvent with ethanol, obtain the standard reserving solution that concentration is 1113mg/L.Internal standard solution seals in 4 ℃ refrigerator and deposits, and is valid for three months;
Accurately pipette 50 μ L, 100 μ L, 500 μ L, 1mL and 5mL standard reserving solution respectively, add 100 μ L inner mark solutions more respectively, be settled in the volumetric flask of 100mL as solvent with ethanol, this series standard working solution concentration is respectively 0.5565mg/L, 1.113mg/L, 5.565mg/L, 11.13mg/L, 55.65mg/L, and interior mark concentration is 1.123mg/L.
(3) preparation of sample solution
Take by weighing 0.5g sample (being accurate to 0.1mg), place 50mL tool plug centrifuge tube, accurately pipette the mixed solvent of 1mL ethanol and 1mL water, vortex mixed scatter sample substrate, the ethanol that adds 8mL again, ultrasonic extraction 20min adds the anhydrous CaCl2 of 1g, leaves standstill 0.5h, extract is centrifugal 5min on hydro-extractor, get supernatant liquor, behind 0.45 μ m water membrane filtration, obtain sample solution.
Do not add sample, handle according to the method described above, get blank solution.
(4) gas chromatography-mass spectrography analysis
Standard operation solution, sample solution and the blank solution of getting above-mentioned 5 variable concentrations respectively carry out the gas chromatography-mass spectrography analysis respectively.Its chromatographiccondition is: adopt DB-5MS(30m * 0.25mm * 0.25 μ m) capillary chromatographic column, carrier gas is helium, sample size is 1 μ L, injector temperature is 260 ℃, adopt the split sampling mode, split ratio is 10:1, and heating schedule is that initial temperature is 60 ℃, speed with 2 ℃/min is increased to 200 ℃, keeps 10min; Its mass spectrophotometry condition is 280 ℃ for the transmission line temperature, the ionization mode is EI, ion source temperature is 230 ℃, the quadrupole rod temperature is 150 ℃, scan mode is selectivity ion scan pattern (SIM), wherein to select ion be 164 to eugenol, and it is 148 that interior mark is selected ion, is 50ms to the monitoring time of each ion.Gained chromatogram such as Fig. 1, Fig. 2 and shown in Figure 3, wherein, Fig. 1 is the chromatogram of standard operation solution, and Fig. 2 is the chromatogram of typical sample, and Fig. 3 is the chromatogram of blank solution.
(5) typical curve is drawn and result's calculating
At first the ratio with eugenol in the standard operation solution and interior target amount is horizontal ordinate, ratio with eugenol in the chromatogram and interior target peak area is ordinate, carry out regretional analysis, obtain typical curve, get the standard operation solution of least concentration, doing 10 parallel detection analyses, calculate its standard deviation, is detection limit with the concentration of 3 times standard deviation correspondences.The regression equation of the eugenol corresponding with standard working curve is Y=0.901X-0.641, and related coefficient is 0.9994, and the range of linearity is 0.5565～55.65 μ g/mL, detects to be limited to 1.15 mg/kg.
Then with eugenol and interior target chromatographic peak area ratio in the sample solution that records under the same terms, the blank solution, the substitution working curve is tried to achieve the content of eugenol in the sample, and computing formula is as follows:
In the formula: X is the content of eugenol in the sample, and unit is every kilogram (mg/kg) of milligram; C is the concentration by eugenol in the sample solution that reads on the standard working curve, and unit is every liter (mg/L) of milligram; C
0Be the concentration by eugenol in the blank solution that reads on the standard working curve, unit is every liter (mg/L) of milligram; V is the volume of extraction system, and unit is milliliter (mL); M is the quality of sample, and unit is gram (g).
20 flavoring essence spices samples choosing in the present embodiment (sample type comprises table perfume, essential oil, sugar material and medicinal extract etc.), except 1 sample detection goes out to contain eugenol (content is 165.02 mg/kg), all the other samples all do not detect and contain eugenol.
Embodiment 2
Present embodiment is as follows to the detection method of precision of the present invention and recovery of standard addition:
Detecting the sample that contains eugenol with embodiment 1 is object, method with reference to embodiment 1, the content of eugenol in 6 replicate determination mark-on samples, calculate relative standard deviation, the precision of investigation method, the result is as shown in table 1, and relative standard deviation RSD has illustrated the good reproducibility of this method less than 2%.
The precision of table 1 method (n=6)
Be object with the above-mentioned sample that contains eugenol that detects, made mark-on samples low, high 3 concentration levels of neutralization respectively, testing result is as shown in table 2.As known from Table 2, the recovery of standard addition of sample has illustrated that the recovery of this method is better between 81.29%～86.95%.
The recovery of standard addition of table 2 method
Standard solution that present embodiment is used is that example describes with one of them concentration only, and the standard solution that other concentration value disposes is same as the previously described embodiments through typical curve and regression equation that chromatograph mass spectrum analysis obtains, is not enumerating one by one at this.Illustrated embodiment is method for a better understanding of the present invention just, does not have any restriction, and namely said method or the method that is equal to above-mentioned situation all are included in the protection domain of technical scheme of the present invention.
Claims (4)
1. the method based on eugenol in the gas chromatography-mass spectrography mensuration flavoring essence spices is characterized in that, comprises the steps:
(1) is internal standard compound with the anethole, makes solvent with ethanol, the preparation inner mark solution;
(2) be reference material with the eugenol, make solvent with ethanol, add inner mark solution respectively, be mixed with series standard solution through stepwise dilution;
(3) accurately take by weighing quantitative flavoring essence spices sample in centrifuge tube, add water and ethanol mixed solvent, vortex mixed scatter sample substrate, adds ethanol again, carries out ultrasonic extraction then; Then, add anhydrous CaCl
2, leave standstill 0.5h, centrifugal on hydro-extractor, get supernatant liquor and cross the organic phase filter membrane, get sample solution; Do not add sample, handle according to the method described above, get blank solution;
(4) with gas chromatograph-mass spectrometer (GCMS) standard operation solution, sample solution and blank solution are detected analysis respectively; Chromatographiccondition is: adopt DB-5MS(30m * 0.25mm * 0.25 μ m) capillary chromatographic column, carrier gas is helium, sample size is 1 μ L, injector temperature is 260 ℃, adopt the split sampling mode, split ratio is 10:1, and heating schedule is that initial temperature is 60 ℃, speed with 2 ℃/min is increased to 200 ℃, keeps 10min; Its mass spectrophotometry condition is 280 ℃ for the transmission line temperature, the ionization mode is EI, ion source temperature is 230 ℃, the quadrupole rod temperature is 150 ℃, scan mode is selectivity ion scan pattern (SIM), wherein to select ion be 164 to eugenol, and it is 148 that interior mark is selected ion, is 50ms to the monitoring time of each ion;
(5) being horizontal ordinate with eugenol in the standard operation solution and interior target concentration ratio, is ordinate with eugenol in the chromatogram and interior target peak area, carries out regretional analysis, obtains typical curve; With eugenol in the sample solution that records under the same terms and interior target chromatographic peak area, the substitution working curve converts according to following computing formula and to try to achieve the content of eugenol in the sample;
Computing formula is:
In the formula: X is the content of eugenol in the sample, and unit is mg/kg; C is the concentration by eugenol in the sample solution that reads on the standard working curve, and unit is mg/L; C0 is the concentration by eugenol in the blank solution that reads on the standard working curve, and unit is mg/L; V is the volume of extraction system, and unit is mL; M is the quality of sample, and unit is g.
2. the method based on eugenol in the gas chromatography-mass spectrography mensuration flavoring essence spices according to claim 1 is characterized in that in the step (1), the concentration of inner mark solution is 1000～2000mg/L.
3. the method for measuring eugenol in the flavoring essence spices based on gas chromatography-mass spectrography according to claim 1 and 2, it is characterized in that, in the step (2), accurately take by weighing 0.1～0.2g reference material eugenol, disperse, dissolve with 2mL methyl alcohol, be settled in the volumetric flask of 100mL with ethanol again, obtain the standard reserving solution that concentration is 1000～2000mg/L; Accurately pipette 50 μ L, 100 μ L, 500 μ L, 1mL and 5mL standard reserving solution respectively, add 100 μ L inner mark solutions more respectively, be settled to as solvent in the volumetric flask of 100mL with ethanol, obtain series standard solution.
4. the method for measuring eugenol in the flavoring essence spices based on gas chromatography-mass spectrography according to claim 1 and 2, it is characterized in that, in the step (5), the regression equation of the eugenol that standard working curve is corresponding is Y=0.901X-0.641, related coefficient is 0.9994, the range of linearity is 0.5565～55.65 μ g/mL, detects to be limited to 1.15 mg/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310287194.3A CN103344734B (en) | 2013-07-10 | 2013-07-10 | Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310287194.3A CN103344734B (en) | 2013-07-10 | 2013-07-10 | Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103344734A true CN103344734A (en) | 2013-10-09 |
| CN103344734B CN103344734B (en) | 2015-01-21 |
Family
ID=49279548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310287194.3A Active CN103344734B (en) | 2013-07-10 | 2013-07-10 | Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103344734B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107192767A (en) * | 2016-03-15 | 2017-09-22 | 中国水产科学研究院 | The method that isotopic dilution gaschromatographic mass spectrometry determines eugenol in aquatic products |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5321005A (en) * | 1992-12-09 | 1994-06-14 | International Flavors & Fragrances Inc. | Flavor and fragrance compositions produced using process for quantitatively and qualitatively substantially continuously analyzing the aroma emitted from a living fruit |
| CN101226178A (en) * | 2008-01-21 | 2008-07-23 | 中国烟草总公司郑州烟草研究院 | Method for analyzing liquid phase fingerprint pattern of cigar flavour and perfume |
| CN102253146A (en) * | 2011-07-14 | 2011-11-23 | 黄石卫生材料药业有限公司 | Determination method of eugenol content in stomach-warming and pain-relieving paste |
| CN102495162A (en) * | 2011-11-16 | 2012-06-13 | 中国烟草总公司郑州烟草研究院 | Method for determining content of eugenol in formulated cut tobacco |
| CN102809621A (en) * | 2012-08-30 | 2012-12-05 | 江苏中烟工业有限责任公司 | Method for detecting content of eugenol in cigarette mainstream smoke |
| CN103105438A (en) * | 2011-11-10 | 2013-05-15 | 中国检验检疫科学研究院 | Method for simultaneously detecting residual amounts of 13 phenolic alcohol sensitization aromatics in toy |
-
2013
- 2013-07-10 CN CN201310287194.3A patent/CN103344734B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5321005A (en) * | 1992-12-09 | 1994-06-14 | International Flavors & Fragrances Inc. | Flavor and fragrance compositions produced using process for quantitatively and qualitatively substantially continuously analyzing the aroma emitted from a living fruit |
| CN101226178A (en) * | 2008-01-21 | 2008-07-23 | 中国烟草总公司郑州烟草研究院 | Method for analyzing liquid phase fingerprint pattern of cigar flavour and perfume |
| CN102253146A (en) * | 2011-07-14 | 2011-11-23 | 黄石卫生材料药业有限公司 | Determination method of eugenol content in stomach-warming and pain-relieving paste |
| CN103105438A (en) * | 2011-11-10 | 2013-05-15 | 中国检验检疫科学研究院 | Method for simultaneously detecting residual amounts of 13 phenolic alcohol sensitization aromatics in toy |
| CN102495162A (en) * | 2011-11-16 | 2012-06-13 | 中国烟草总公司郑州烟草研究院 | Method for determining content of eugenol in formulated cut tobacco |
| CN102809621A (en) * | 2012-08-30 | 2012-12-05 | 江苏中烟工业有限责任公司 | Method for detecting content of eugenol in cigarette mainstream smoke |
Non-Patent Citations (7)
| Title |
|---|
| MARI´A J. JORDA´N ET AL: "Aromatic Profile of Aqueous Banana Essence and Banana Fruit by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Olfactometry (GC-O)", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| S.C. RASTOGI ET AL: "Contents of fragrance allergens in children’s cosmetics and cosmetic-toys", 《CONTACT DERMATITIS》 * |
| 廖惠云 等: "涡旋混合辅助超声提取-气质联用法分析食品添加剂中的咪唑", 《食品科学》 * |
| 曲国福 等: "GC/MS法建立烟用香精指纹图谱研究", 《香料香精化妆品》 * |
| 曲国福 等: "色谱指纹图谱在烟用香精质量控制中的应用", 《烟草科技》 * |
| 王超 等: "气相色谱-质谱法分析化妆品中16种香精香料", 《分析试验室》 * |
| 綦艳梅 等: "美拉德反应制备酱牛肉香精的挥发性成分分析", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107192767A (en) * | 2016-03-15 | 2017-09-22 | 中国水产科学研究院 | The method that isotopic dilution gaschromatographic mass spectrometry determines eugenol in aquatic products |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103344734B (en) | 2015-01-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102809621B (en) | Method for detecting content of eugenol in cigarette mainstream smoke | |
| Farag et al. | The characterization of flavored hookahs aroma profile and in response to heating as analyzed via headspace solid-phase microextraction (SPME) and chemometrics | |
| CN109444284B (en) | Method for simultaneously determining flavor substances in pepper and/or rattan pepper and oil products thereof | |
| CN103869039B (en) | A kind of method simultaneously detecting seven kinds of forbidding compounds in essence and flavoring agent | |
| CN102353736A (en) | Method for determining menthol in cigarette filter rod | |
| Da et al. | Characterization of specific volatile components in braised pork with different tastes by SPME‐GC/MS and electronic nose | |
| CN106353419A (en) | Method for measuring aroma components in main stream smoke of cigarettes | |
| CN106749143A (en) | A kind of method that scopolactone compound is extracted from tobacco | |
| CN102879509A (en) | Method for measuring content of phthalate in cigarette filter rod or finished cigarette filter tip | |
| CN103558319B (en) | Method for measuring methyl eugenol in mainstream smoke of cigarettes | |
| CN103399089B (en) | Method for measuring eugenol in edible flavour and fragrance based on high performance liquid chromatography | |
| CN103344734B (en) | Method for measuring eugenol in edible flavor and fragrance based on gas chromatography-mass spectrography | |
| CN102495162B (en) | Method for determining content of eugenol in formulated cut tobacco | |
| CN104090040A (en) | HPLC method for rapidly detecting main active ingredients of lithocarpus polysachyus rehd | |
| CN105954434B (en) | A kind of detection method of phenols fragrance | |
| CN104090043B (en) | A kind of method measuring methyl eugenol in cigarette mainstream flue gas | |
| CN104777256B (en) | Analysis method for simultaneously determining six polyphenol contents in flue-cured tobacco | |
| Piovetti et al. | Determination of sterols and diterpenoids from brown algae (Cystoseiraceae) | |
| CN103336085B (en) | High performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke | |
| CN104090042A (en) | Method for measuring safrole in mainstream smoke of cigarettes | |
| CN109613141A (en) | The detection method of gamma lactone and delta-lactone class compound in a kind of quick-fried pearl liquid of cigarette filter tip | |
| CN108414666A (en) | The assay method of gingerol content in a kind of ginger medicinal material extractive of volatile oil | |
| CN104237428B (en) | The assay method of menthol in a kind of smoke-free tobacco product | |
| CN101592638B (en) | Method for separating and detecting harmful phenols in main stream smoke of cigarettes | |
| CN108088922A (en) | Fragrance component database in essence rapid screening database and tealeaves in essence high flux examination detection method, tealeaves in tealeaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |