CN103344618B - Fluorescence excitation testing device - Google Patents
Fluorescence excitation testing device Download PDFInfo
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- CN103344618B CN103344618B CN201310260290.9A CN201310260290A CN103344618B CN 103344618 B CN103344618 B CN 103344618B CN 201310260290 A CN201310260290 A CN 201310260290A CN 103344618 B CN103344618 B CN 103344618B
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- camera bellows
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- fluorescence excitation
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- 230000005284 excitation Effects 0.000 title claims abstract description 99
- 238000012360 testing method Methods 0.000 title claims abstract description 80
- 230000003287 optical effect Effects 0.000 claims abstract description 29
- 239000000523 sample Substances 0.000 claims description 54
- 239000000463 material Substances 0.000 claims description 26
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims description 4
- 230000007246 mechanism Effects 0.000 claims description 4
- 238000010183 spectrum analysis Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 abstract description 4
- 238000001228 spectrum Methods 0.000 description 12
- 239000000843 powder Substances 0.000 description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 5
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- 230000005540 biological transmission Effects 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
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- 230000003247 decreasing effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a fluorescence excitation testing device. The fluorescence excitation testing device comprises a camera obscura used for light shielding, a sample tray which is arranged at the top of the camera obscura and used for placing a tested sample, a plurality of excitation light sources arranged at the bottom of the camera obscura, as well as an optical receiver which is arranged between the sample tray and the excitation light source and positioned in the middle of the camera obscura, wherein the bottom of the sample tray is transparent, the plurality of excitation light sources are symmetrically distributed at the bottom of the camera obscura and emit excitation light which irradiates onto the tested sample in the sample tray through the transparent bottom of the sample tray and excites the tested sample to emit excited light, and the optical receiver is used for collecting mixed light of the excitation light and the excited light. According to the fluorescence excitation testing device disclosed by the invention, measurement results are accurate and have very high reference values.
Description
Technical field
The present invention relates to photoelectric detection technology field, refer to a kind of fluorescence excitation testing arrangement especially.
Background technology
Fluorescence excitation testing arrangement, is luminous by excitation test substance, and tests the spectrum of its light that is excited (being generally fluorescence), to record the testing arrangement of the optical parameter of this test substance.
With reference to accompanying drawing 1, it is the structural representation of the fluorescence excitation testing arrangement of prior art.
The fluorescence excitation testing arrangement of prior art, comprise excitation source 101, for the camera bellows 102 exposed and measure, in camera bellows 102, adjustable diaphragm 103 is set below excitation source 101, below excitation source 101, be provided with the powder dish 104 placing fluorescent powder samples, camera bellows 102 is provided with the utilizing emitted light receiving system 105 that measuring instrument is connected.
This testing arrangement is luminous by excitation source 101, the fluorescent powder samples in powder dish 104 is irradiated to through adjustable diaphragm 103, this fluorescent powder samples is subject to light source activation and launches fluorescence, received the spectrum of the mixed light of light source and described fluorescence by utilizing emitted light receiving system 105, and finally obtain every optical parameter of fluorescent powder samples.
The shortcoming of this testing arrangement is: before test, need fluorescent material to be added in powder dish 104, by fluorescent material compacting, and utilize aid to be struck off by the upper surface of fluorescent material, after arriving fluorescent material upper surface to make light source irradiation, the fluorescence be excited can uniform emission; But there is a lot of defect in this method:
1) because this dress quadrat method must fluorescent material compacting, otherwise phosphor surface can out-of-flatness; But because the strength of each manual compaction is different, phosphor surface density can be caused different, in the test of not homogeneous like this, when exciting light is irradiated to phosphor surface, then can there is error in the fluorescent material utilizing emitted light produced that is stimulated.
2) be difficult to the flatness ensureing phosphor surface, often there will be cut or pit, there is deviation in the utilizing emitted light direction after this just causes fluorescent material to be excited.Meanwhile, because utilizing emitted light receiving system 105 and light source axis are that miter angle is arranged, the deviation of the light causing utilizing emitted light receiving system 105 to receive increases.Further, excitation source only has one, and it is uneven that this also can cause being irradiated to phosphor surface irradiance.
3) due to each to the difference of fluorescent material compaction force, can cause the position of phosphor surface have height point, therefore, when testing, the fluorescent material radiative transmission range produced that is stimulated is then different at every turn; According to langbobier law, light loss square distance is inversely proportional to, and therefore, between each test, the difference of light loss then can cause the error of test result.
In sum, adopt prior art to carry out fluorescence excitation test, its final measuring result error is comparatively large, can referential greatly reduce.
Summary of the invention
In view of this, the object of the invention is to propose a kind of fluorescence excitation testing arrangement, its measurement result is accurate, has very high reference value.
Based on above-mentioned purpose fluorescence excitation testing arrangement provided by the invention, comprise for light shield camera bellows, be arranged at described camera bellows top for place tested sample platter, be arranged at multiple excitation sources bottom described camera bellows, and to be arranged between described platter and excitation source and the optical receiver be positioned in the middle part of described camera bellows; Wherein, be transparent bottom described platter, described multiple excitation source is symmetrically distributed in bottom described camera bellows; Described multiple excitation source sends exciting light, is irradiated to tested sample wherein and excites it to send the light that is excited through described platter clear bottom, and described optical receiver gathers described exciting light and the mixed light of the light that is excited.
In some embodiments, described multiple excitation source forms an annular light source, and the center of this annular light source is just to described platter.
In some embodiments, the general power of described annular light source is 15W.
In some embodiments, radiator is provided with below described camera bellows.
In some embodiments, described optical receiver is spectrum analysis instrument probe.
In some embodiments, described camera bellows open top, described platter is open-topped hollow cylinder, and described hollow cylinder outer surface and described camera bellows open top inner surface closely cooperate; Be provided with around described platter top and protrude from described cylindrical for stopping the blocking mechanism that described platter falls, make described platter steadily can be positioned over described camera bellows top.
In some embodiments, be made up of quartz glass bottom described platter.
In some embodiments, described fluorescence excitation testing arrangement also comprises calibrating plate; The contour structures of described calibrating plate is identical with described platter; Described calibrating plate also comprises the fluorescent material of the known parameters be placed therein.
In some embodiments, described calibrating plate also comprises the cover plate for being blocked in by the fluorescent material of described known parameters within described calibrating plate, makes described fluorescent material keep certain thickness and can not spread out.
As can be seen from above, fluorescence excitation testing arrangement provided by the invention, only needs tested sample to be tested to be placed in described platter, opens excitation source and irradiates it, excite it to produce to be excited light, and adopt described optical receiver to carry out spectra collection can to complete test.
Its advantage is, when placing tested sample, without the need to tested sample surface aid is struck off, because only irradiate from the bottom up during test, owing to being transparent and smooth bottom platter, the lower surface of tested sample then maintains smooth, when can ensure that exciting light is irradiated to the lower surface of tested sample, can not because of having pit or cut and producing deviation, its measurement result is more accurate; Meanwhile, owing to decreasing the step struck off by tested sample upper surface with aid, then improve testing efficiency.
Further, adopt fluorescence excitation testing arrangement provided by the present invention, directly tested sample being put into described platter can test, and without the need to pressing tested sample, tested sample is very even; When therefore filling sample, its density is substantially identical at every turn, there will not be dress sample error between each test, improves dress sample precision.
Have again, adopt fluorescence excitation testing arrangement provided by the present invention, tested sample present position, surface immobilizes because the position of platter is certain, therefore, its light loss at every turn tested is all substantially identical, further reduces the error not between homogeneous test result.
Further, by adopting annular light source to carry out uniform irradiation to tested sample, compared to the single light source of prior art, its light source general power is less, improves illumination efficiency.
Preferably, bottom the platter of fluorescence excitation testing arrangement provided by the present invention, adopt quartz glass, its uniform in material degree and transmitance higher, be conducive to tested sample excite and the later stage to the process of test result, computation and analysis.
Accompanying drawing explanation
Fig. 1 is the structural representation of the fluorescence excitation testing arrangement of prior art;
The cross-sectional view of the fluorescence excitation testing arrangement that Fig. 2 provides for the embodiment of the present invention;
The annular light source top view of the fluorescence excitation testing arrangement that Fig. 3 provides for the embodiment of the present invention;
The calibrating plate generalized section of the fluorescence excitation testing arrangement that Fig. 4 provides for the embodiment of the present invention.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in more detail.
One embodiment of the invention provides a kind of fluorescence excitation testing arrangement, comprise for light shield camera bellows, be arranged at described camera bellows top for place tested sample platter, be arranged at multiple excitation sources bottom described camera bellows, and to be arranged between described platter and excitation source and the optical receiver be positioned in the middle part of described camera bellows; Wherein, be transparent bottom described platter, described multiple excitation source is symmetrically distributed in bottom described camera bellows; Described multiple excitation source sends exciting light, is irradiated to tested sample wherein and excites it to send the light that is excited through described platter clear bottom, and described optical receiver gathers described exciting light and the mixed light of the light that is excited.
With reference to accompanying drawing 2 and accompanying drawing 3, be respectively the cross-sectional view of the fluorescence excitation testing arrangement that the embodiment of the present invention provides and the annular light source top view of fluorescence excitation testing arrangement.
Described fluorescence excitation testing arrangement, comprise for light shield camera bellows 202, be arranged at described camera bellows 202 top for place tested sample 211 platter 204, be arranged at multiple excitation sources 201 bottom described camera bellows 202, and to be arranged between described platter 204 and excitation source 201 and the optical receiver 205 be positioned in the middle part of described camera bellows 202; Wherein, bottom described platter, 206 is transparent, and described multiple excitation source 201 is symmetrically distributed in bottom described camera bellows 202; Be provided with the first through hole 214 bottom described camera bellows 202, enter in described camera bellows 202 through this first through hole 214 for described optical receiver 205 and carry out light collection.
Described multiple excitation source 201 sends exciting light, is irradiated to tested sample 211 wherein and excites it to send the light that is excited through described platter clear bottom 206, and described optical receiver 205 gathers described exciting light and the mixed light of the light that is excited.Described optical receiver 205 is spectrum analysis instrument probe, and the spectral information of described mixed light is by this probe acquires in described spectroanalysis instrument 207, and the curve of spectrum obtaining corresponding mixed light obtains every optical parameter of tested sample for subsequent optical analysis of spectrum.
Further, described multiple excitation source 201 forms a circle to form an annular light source (with reference to accompanying drawing 3), and the center of this annular light source is just to described platter 204 bottom centre.Described annular light source is arranged on light source base 203, described light source base 203 to be arranged at bottom camera bellows 202 and to be provided with the second through hole 208 bottom it, its position is corresponding with described first through hole 214, enters in described camera bellows 202 carry out light collection for described optical receiver 205 through this first through hole 214 and the second through hole 208.Be provided with hollow base 210 below described camera bellows 202, coincide bottom a part for described hollow base 210 upper surface and described camera bellows 202 thus form as one; Radiator 209 is provided with, for carrying out radiating treatment to excitation source 201 in described hollow base 210.
Preferably, the general power of described annular light source is 15W.
The open top of described camera bellows 202, described platter 204 is open-topped hollow cylinder 212, and described hollow cylinder 212 outer surface and described camera bellows 202 open top inner surface closely cooperate; Be provided with around described platter 204 top protrude from described hollow cylinder 212 for stopping the blocking mechanism 213 that described platter 204 falls; Make the hollow cylinder 212 of described platter 204 to pass the open top of described camera bellows 202 and to stretch within camera bellows 202, and described platter 204 steadily can be positioned over described camera bellows 202 top.
Preferably, 206 to be made up of the transparent material of high surface smoothness bottom described platter; Preferably, be made up of the transparent material of known refraction parameter and transmission parameters.Preferably, quartz glass is adopted to make bottom described platter 206.
Optionally, described optical receiver 205 is fiber optic receiver.
Further, described fluorescence excitation testing arrangement also comprises calibrating plate 301.With reference to accompanying drawing 4, it is the calibrating plate generalized section of the fluorescence excitation testing arrangement that the embodiment of the present invention provides.The contour structures of described calibrating plate 301 is identical with described platter 204; Comprising, bottom open-topped calibrating plate hollow cylinder 305, calibrating plate 302, and protrude from described calibrating plate hollow cylinder 305 for stopping the calibrating plate blocking mechanism 306 that described calibrating plate 301 falls; Described calibrating plate 301 also comprises fluorescent material 304 and the cover plate 303 of the known parameters be placed therein, and described fluorescent material 304 is blocked within described calibrating plate 301 by described cover plate 303, makes described fluorescent material 304 keep certain thickness and can not spread out.
Before the test begins, first described calibrating plate 301 is positioned over camera bellows 202 top, it is made to launch exciting light to excitation source 201 energising, described exciting light upwards irradiates, 302 lower surfaces being irradiated to the fluorescent material 304 of known parameters bottom described calibrating plate, described known parameters fluorescent material 304 is subject to exciting of exciting light, launch the light that is excited (i.e. fluorescence), 302 to be irradiated to camera bellows 202 inner bottom described calibrating plate for this light that is excited, the described optical receiver 205 be positioned in the middle part of camera bellows gathers described exciting light and the mixed light of the light that is excited, and the spectral information of described mixed light is collected in described spectroanalysis instrument 207, obtain the curve of spectrum of corresponding mixed light.After spectrum analysis, obtain every optical parameter of described known parameters fluorescent material 304, the light stable parameter recorded is contrasted, thus learn whether described fluorescence excitation testing arrangement is calibrated before this optical parameter and its; If parameter is inconsistent, then finely tune to calibrate to this fluorescence excitation testing arrangement.
After described fluorescence excitation testing arrangement calibration, the test to tested sample 211 can be started.
Tested sample 211 is put into described platter 204, described platter 204 is positioned over camera bellows 202 top, it is made to launch exciting light to excitation source 201 energising, described exciting light upwards irradiates, 206 lower surfaces being irradiated to tested sample 211 bottom described platter, described tested sample 211 is subject to exciting of exciting light, launch the light that is excited (i.e. fluorescence), 206 to be irradiated to camera bellows 202 inner bottom described platter for this light that is excited, the described optical receiver 205 be positioned in the middle part of camera bellows gathers described exciting light and the mixed light of the light that is excited, and the spectral information of described mixed light is collected in described spectroanalysis instrument 207, obtain the curve of spectrum of corresponding mixed light.
By analyzing the curve of spectrum obtained, remove exciting light spectrum, and according to bottom described platter 206 the known refraction parameter of making material and transmission parameters, remove 206 impacts produced bottom described platter, the curve of spectrum that is excited of described tested sample 211 can be obtained, thus obtain corresponding optical parameter according to this curve of spectrum.
Preferably, the excitation source 201 of described fluorescence excitation testing arrangement is monochromatic LED light source, and described tested sample 211 is for being different from the one-color fluorescence powder of excitation source color; Preferably, the excitation source 201 of described fluorescence excitation testing arrangement is blue led, and described tested sample 211 is the yellow fluorescent powder of unknown parameter.
As can be seen from above-described embodiment, fluorescence excitation testing arrangement provided by the invention is adopted to carry out fluorescence excitation test, tested sample to be tested is only needed to be placed in described platter, open excitation source to irradiate it, excite it to produce to be excited light, and adopt described optical receiver to carry out spectra collection can to complete test.Its advantage is, when placing tested sample, without the need to tested sample surface aid is struck off, because only irradiate from the bottom up during test, owing to being transparent and smooth bottom platter, the lower surface of tested sample then maintains smooth, when can ensure that exciting light is irradiated to the lower surface of tested sample, can not because of having pit or cut and producing deviation, its measurement result is more accurate; Meanwhile, owing to decreasing the step struck off by tested sample upper surface with aid, then improve testing efficiency.
Further, adopt fluorescence excitation testing arrangement provided by the present invention, directly tested sample being put into described platter can test, and without the need to pressing tested sample, tested sample is very even; When therefore filling sample, its density is substantially identical at every turn, there will not be dress sample error between each test, improves dress sample precision.
Have again, adopt fluorescence excitation testing arrangement provided by the present invention, tested sample present position, surface immobilizes because the position of platter is certain, therefore, its light loss at every turn tested is all substantially identical, further reduces the error not between homogeneous test result.
Further, be a circle by excitation source arrangement, form annular light source, exciting light uniform irradiation can be made to tested sample lower surface, make it be subject to uniform fluorescence excitation, and annular light source also makes excitating light strength stronger compared with the intensity of single point light source.Preferably, by excitation source is set on light source base, the light source base with different capacity excitation source can be designed as required, the excitation source of band base is replaced by entirety, can realize adopting different capacity excitation source to irradiate tested sample, make the use of described fluorescence excitation testing arrangement easier.Meanwhile, by adopting annular light source to carry out uniform irradiation to tested sample, compared to the single light source of prior art, its light source general power is less, improves illumination efficiency.
Further, arrange described radiator below described camera bellows, the heat that described excitation source can be sent is discharged, to ensure excitation source steady operation.
Preferably, bottom described camera bellows, through hole is set, make described optical receiver can free in and out this camera bellows, and its collection position is also therefore adjustable, be more conducive to testing experiment and device replacing.
Preferably, be provided with described calibrating plate, for the calibration before testing, improve measuring accuracy.
Preferably, bottom the platter of fluorescence excitation testing arrangement provided by the present invention, adopt quartz glass, its uniform in material degree and transmitance higher, be conducive to tested sample excite and the later stage to the process of test result, computation and analysis.
It should be noted that the tested sample of described fluorescence excitation testing arrangement can be fluorescent material, or other similar fluorescent materials, only have and can carry out fluorescence excitation test with fluorescence excitation testing arrangement provided by the invention, be tested sample of the present invention.
Each additional technical feature described in above-described embodiment, as profile or the structure of annular light source, light source base, bottom through-hole, radiator, calibrating plate etc., the technique effect reached is additional technical effect of the present invention, therefore, they should not limited protection scope of the present invention as essential features of the present invention; Certainly the arbitrary arrangement combination of these additional technical features also should belong to scope; do not repeat them here the embodiment that these are possible, those skilled in the art should predict other detailed description of the invention that these permutation and combination obtain from the content described in above-described embodiment.
Those of ordinary skill in the field are to be understood that: the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. a fluorescence excitation testing arrangement, comprising camera bellows, excitation source, optical receiver, for placing the platter of tested sample, it is characterized in that,
Described camera bellows is used for light shield, and described platter is arranged at described camera bellows top, and multiple excitation source is arranged at bottom described camera bellows, and described optical receiver to be arranged between described platter and excitation source and to be positioned in the middle part of described camera bellows;
Wherein, be transparent bottom described platter, described multiple excitation source is symmetrically distributed in bottom described camera bellows; Described multiple excitation source sends exciting light, is irradiated to tested sample wherein and excites it to send the light that is excited through described platter clear bottom, and described optical receiver gathers described exciting light and the mixed light of the light that is excited.
2. fluorescence excitation testing arrangement according to claim 1, is characterized in that, described multiple excitation source forms an annular light source, and the center of this annular light source is just to described platter.
3. fluorescence excitation testing arrangement according to claim 1, is characterized in that, the general power of described multiple excitation source is 15W.
4. fluorescence excitation testing arrangement according to claim 1, is characterized in that, is provided with radiator below described camera bellows.
5. fluorescence excitation testing arrangement according to claim 1, is characterized in that, described optical receiver is spectrum analysis instrument probe.
6. the fluorescence excitation testing arrangement according to claim 1-5 any one, it is characterized in that, described camera bellows open top, described platter is open-topped hollow cylinder, and described hollow cylinder outer surface and described camera bellows open top inner surface closely cooperate; Be provided with around described platter top and protrude from described cylindrical for stopping the blocking mechanism that described platter falls, make described platter steadily can be positioned over described camera bellows top.
7. fluorescence excitation testing arrangement according to claim 6, is characterized in that, is made up bottom described platter of quartz glass.
8. fluorescence excitation testing arrangement according to claim 7, is characterized in that, described fluorescence excitation testing arrangement also comprises calibrating plate; The contour structures of described calibrating plate is identical with described platter; Described calibrating plate also comprises the fluorescent material of the known parameters be placed therein.
9. fluorescence excitation testing arrangement according to claim 8, is characterized in that, described calibrating plate also comprises the cover plate for being blocked in by the fluorescent material of described known parameters within described calibrating plate, makes described fluorescent material keep certain thickness and can not spread out.
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| CN108535226A (en) * | 2018-03-19 | 2018-09-14 | 厦门大学 | A kind of flourescent sheet transmitting optical property test device and method based on multichannel probe |
| CN111413307A (en) * | 2020-04-09 | 2020-07-14 | 安徽环球基因科技有限公司 | Up-conversion fluorescence detection device and detection method |
| CN113418897B (en) * | 2021-06-17 | 2023-10-20 | 安徽建筑大学 | Fluorescent material excitation measuring device |
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| US6867851B2 (en) * | 1999-11-04 | 2005-03-15 | Regents Of The University Of Minnesota | Scanning of biological samples |
| CN2537010Y (en) * | 2001-11-21 | 2003-02-19 | 浙江大学 | Instrument for measuring long persistence fluorescent materials |
| CN100594371C (en) * | 2003-01-16 | 2010-03-17 | 北京师范大学 | Automatic testing device and its testing method for luminuoas characteristics of long-afterglow fluorescent powder |
| FI20030867A (en) * | 2003-06-10 | 2004-12-11 | Wallac Oy | Optical measuring method and laboratory measuring apparatus |
| CN200968935Y (en) * | 2006-11-21 | 2007-10-31 | 杭州远方光电信息有限公司 | Exciting and receiving apparatus for luminous diode fluorescent powder test |
| CN201034939Y (en) * | 2007-04-13 | 2008-03-12 | 杭州远方光电信息有限公司 | Fluorescent powder excited measurement mechanism |
| CN201043952Y (en) * | 2007-04-13 | 2008-04-02 | 杭州远方光电信息有限公司 | Measuring device for fluorescent powder excitation spectrum |
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