CN103340090B - Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer - Google Patents
Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer Download PDFInfo
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- CN103340090B CN103340090B CN201310235162.9A CN201310235162A CN103340090B CN 103340090 B CN103340090 B CN 103340090B CN 201310235162 A CN201310235162 A CN 201310235162A CN 103340090 B CN103340090 B CN 103340090B
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Abstract
Disclosed is a method for culturing a mushroom kernel microbial strain, namely, a kernel cultivated specie, by using white soil as a filter layer. The method for culturing the kernel cultivated specie is characterized by including the processing steps that materials are prepared, wherein the step includes the sub-steps of processing and culturing kernel culture media for standby application and taking enough natural white soil for standby application; the water content of the white soil is adjusted to 24%-27%, and the pH value of the white soil is adjusted to 7.3-7.7; the kernel culture media are filled into a culture bottle in a conventional method, and then the white soil is filled into the culture bottle, wherein the white soil is located above the kernel culture media, the distance between the white soil and a bottleneck is 4-5cm, and the thickness of the white soil is 1.5-2cm; inoculation is carried out after sterilization treatment; then the culture bottle is placed in a culture room, and the cultivated specie is cultured in a conventional method. The method has the effect of filtering contaminating microorganisms and can avoid contaminating microorganism contamination in the culturing process to the maximum, the rate of finished products can reach 99%-100%, besides, materials for manufacturing the filer layer can be easily obtained, and cost of the filter layer is low.
Description
The present invention is the divisional application for " utilizing white loam to make the method for filter course making mushroom and Ji Songrong kernel culture " by denomination of invention, and its applying date is on 01 09th, 2012, and application number is 201210013247.8.
Technical field
The present invention relates to a kind of edible fungus species manufacturing technology, particularly a kind of method of utilizing white loam to make filter course making mushroom kernel cultivated species.
Background technology
As everyone knows, the successful crucial prerequisites of edible mushroom such as artificial cultivation Ji matsutake are bacterial classifications, only have good bacterial classification just can reach good quality and high output, the edible fungus species such as mushroom and Ji Songrong manufacturing process comprises: first stage is to produce female kind, and namely from fruit body, purification and rejuvenation obtain female kind (being called again one-level kind); Second stage is to produce original seed, refers to mother is planted and expands numerous original seed that arrives, and namely utilizes in female mycelium access pedigree seed culture medium of planting, and expanding propagation is cultivated into original seed (secondary kind); Three phases is to produce cultivated species, there is original seed not enough, next step is also it will be expanded to cultivated species (i.e. three grades of kinds) in the phase III, and cultivated species is exactly the mycelium bacterial classification (become the bacterial classification that can directly put into production) of expanding propagation after once again that utilizes original seed.Due in the bacterial classification such as mushroom and Ji Songrong (referring to original seed or cultivated species) manufacturing process, be difficult to the sterile working state that reaches real, living contaminants rate reaches 20%-30%, even up to more than 50%, in order to reduce bacterial classification pollution rate, improve bacterial classification yield rate, several measure methods that reduce at present the edible fungus species such as mushroom and Ji Songrong pollution rate are:
One of measure, the general method that adopts larger inoculum concentration.Allow and need the bacterial classification of cultivating to have the mycelia of q.s, in the time getting along with the miscellaneous bacteria that may infiltrate, utilize and be total to the growth that growth vigor suppresses miscellaneous bacteria, reach the effect that improves bacterial classification yield rate.
Two of measure, bottleneck dress fermentation material (1cm left and right thickness).Be exactly above medium from about bottleneck 4cm, pack (making through special fermentation) fermentation material of 1-2cm thickness into, then sterilizing.Because fermentation material itself is aseptic, after access bacterial classification, mycelia is covered with very soon the top of whole bottle in material feeding, easily forms growth vigor altogether and suppresses miscellaneous bacteria, and this method can largely reduce pollution rate, and shortcoming is complicated operation, time-consuming taking a lot of work.
Though use the above-mentioned measure method that these reduce bacterial classification pollution rate to there is in the ordinary course of things the good effect that improves bacterial classification yield rate, but due in traditional original seed (secondary kind) such as making mushroom and Ji Songrong and cultivated species (three grades of kinds) process, to adopt straw or cotton seed hull or the cow dung etc. fermented easily to keep the fermentation material material of moisture content to sprout the rear cultural hypha matrix that forms as the inoculation piece of mother's kind or original seed, these cultural hypha matrix are as guiding material (note: these inoculation piece materials that form cultural hypha matrix are often called guiding material by people), by reboot mycelia from original inoculation piece to new cultivation matrix direction growth using favourable formation growth vigor (note: be all generally that some are nutritious and have fermentation material that the raw material of good moisture-retaining capacity makes through special fermentation with favourable formation growth vigor altogether as the material of guiding material) altogether, can be found out by the above, due in large-scale bacterial classification manufacturing process, especially in seeded process, be difficult to the sterile working state that reaches real, this has just produced great negative effect to the practices well of utilizing the common growth vigor of bacterial classification mycelia to suppress varied bacteria growing, not only reduce bacterial classification pollution rate limited, and complex operation.
Separately, along with the reach of science, the traditional grass of the excrement for the edible mushroom such as mushroom and Ji Songrong bacterial classification (claiming again excrement grass cultivated species) is in superseded edge, then and emerging be to make the kernel cultures such as mushroom and Ji Songrong as original seed and produce to arise at the historic moment with cultivated species, the edible mushroom such as mushroom and Ji Songrong kernel culture has with short production cycle, simple process, cell age is consistent, strong stress resistance, sowing is convenient, be difficult for old and feeble, compared with advantages such as storage tolerances, so adopt the above-mentioned measure method that these reduce pollution rates make when kernel culture more aobvious particularly important, but manufacturing process is also difficult to accomplish real sterile working equally, also tend to enrich because of guiding material nutrient, also the growth of material feeding is simultaneously very fast for miscellaneous bacteria mycelia, thereby greatly weaken the originally altogether bacterial classification mycelia of growth vigor and play the effect that suppresses varied bacteria growing, cause the original seed of manufacturing out, the yield rate of cultivated species is low, seriously restrict and affected productivity effect.
Summary of the invention
In view of kernel culture has inoculation conveniently, sowing quantity is little, mycelium germination is fast, growing way is prosperous, can within very short time, form biocenose advantage at charge level, reduce living contaminants, be suitable for the advantages such as large-scale production, task of the present invention is to provide a kind of method of utilizing white loam to make filter course making mushroom kernel cultivated species, make to play the effect of filtering miscellaneous bacteria, can at utmost overcome the living contaminants phenomenon (miscellaneous bacteria is difficult for contaminating medium) in the process of manufacturing, also make the cell age of bacterial classification consistent, and this filter course material used is drawn materials easily, cost is light, bacterial classification pollution rate reduces effective.
The present invention realizes with following technical scheme, the white loam of this utilization of the present invention is made the method for filter course making mushroom kernel cultivated species, the method still comprises following processing step: the one, produce female kind (claiming again one-level kind), the 2nd, produce (wheat) original seed (claiming again secondary kind), the 3rd, produce (wheat) cultivated species and (claim again three grades of kinds, for the bacterial classification that can directly put into production), it is characterized in that: the processing step of wherein said production (wheat) cultivated species (claim not only three grades of kinds) but also comprise: get the raw materials ready, comprise that processing and fabricating wheat medium is for subsequent use and (from mountain) to get the natural white loam of q.s for subsequent use, white loam water content is transferred to 24-27%, and pH value is transferred to 7.3-7.7 (as 7.5 left and right), according to conventional method, by wheat medium bottling (packing in blake bottle), (from bottle mouth position) reinstalls white loam afterwards, and the white loam that makes to pack into is positioned at the top of wheat medium and apart from bottleneck 4-5cm, white loam thickness is 1.5-2cm, after sterilization treatment, inoculate, access original seed (claiming again secondary kind), afterwards the blake bottle that connects original seed is positioned over to the cultivation of carrying out cultivated species in culturing room according to conventional method, after wheat cultivated species is carried out, just can becomes the bacterial classification directly putting into production and be used for having sent out bacterium fruiting.
In the present invention, the processing and fabricating of described wheat medium can, according to conventional method step, comprise: the steps such as immersion, pre-steaming, airing, spice bottling (wheat kind is made container can use 750 milliliters of glass seed bottle), sterilizing (becoming wheat medium after sterilizing) are made.
Principle of the present invention and beneficial effect are: the filter course while utilizing white loam on mountain to make as mushroom kernel cultivated species, make guiding material originally change into filter course, can at utmost overcome living contaminants phenomenon in manufacturing process (polluting hardly), yield rate can reach 99%-100%, its principle is exactly in white loam, not contain organic matter, make miscellaneous bacteria silk in this soil, be difficult to breeding growth, loam but has good moisture-retaining capacity in vain, so in mushroom kernel cultivated species manufacturing process, utilize white loam as the filter course above wheat, can play the effect of filtering miscellaneous bacteria, but little on the normal growth impact of bacterial classification mycelia, because containing enough moisture in white loam, the mycelia that inoculation piece is sprouted can reach wheat medium by (passing) filter course smoothly, even if be also difficult to grow in this filter course being formed by white loam and there is miscellaneous bacteria spore etc. to enter into, and this filter course material used is drawn materials easily, cost is light, effective.
Embodiment
Below to utilize technical solution of the present invention for making a kind of specific embodiment of mushroom kernel cultivated species.
The white loam of this utilization of the present invention is made the method for filter course making mushroom kernel cultivated species, the method still comprises following processing step: the one, produce female kind (claiming again one-level kind), the 2nd, and produce (wheat) original seed (secondary kind), the 3rd, produce (wheat) cultivated species (three grades of kinds, for the bacterial classification that can directly put into production), it is characterized in that: the processing step of wherein said production (wheat) cultivated species (claiming again three grades of kinds) is: get the raw materials ready, comprise processing and fabricating wheat medium and get the natural white loam of q.s from mountain for subsequent use; White loam water content is transferred to 24-27%, and pH value is transferred to 7.3-7.7 (as being transferred to 7.5 left and right); According to conventional method, wheat medium is bottled (packing in blake bottle), (from bottle mouth position) reinstalls white loam afterwards, the white loam that makes to pack into is positioned at the top of wheat medium and apart from bottleneck 4-5cm left and right, white loam thickness is 1.5-2cm; After sterilization treatment, inoculate, access original seed (claiming again secondary kind); Afterwards the blake bottle that connects original seed is positioned over to the cultivation of carrying out cultivated species in culturing room according to conventional method, wheat cultivated species just can become the bacterial classification directly putting into production and be used for having sent out bacterium fruiting after carrying out.
Claims (2)
1. a method of utilizing white loam to make filter course making mushroom kernel cultivated species, the method comprises following processing step: the one, produce female kind, the 2nd, and produce wheat original seed, the 3rd, produce wheat cultivated species, it is characterized in that: the processing step of wherein said production wheat cultivated species comprises again: get the raw materials ready, comprise that processing and fabricating wheat medium is for subsequent use and get the natural white loam of q.s from mountain for subsequent use; White loam water content is transferred to 24-27%, and pH value is transferred to 7.3-7.7; By wheat medium bottling, reinstall white loam according to conventional method afterwards from bottle mouth position, the white loam that makes to pack into is positioned at the top of wheat medium and apart from bottleneck 4-5cm, white loam thickness is 1.5-2cm; After sterilization treatment, inoculate, access original seed; Afterwards the blake bottle that connects original seed is positioned over to the cultivation of carrying out cultivated species in culturing room according to conventional method.
2. the white loam of utilization according to claim 1 is made the method for filter course making mushroom kernel cultivated species, it is characterized in that: the processing and fabricating of described wheat medium is according to conventional method step, comprising: after immersion, pre-steaming, airing, spice bottling, sterilizing, become wheat medium.
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| CN201310235162.9A CN103340090B (en) | 2012-01-09 | 2012-01-09 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
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| CN201310235162.9A CN103340090B (en) | 2012-01-09 | 2012-01-09 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
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| CN201210013247.8A Division CN102523926B (en) | 2012-01-09 | 2012-01-09 | Method for culturing strain of Agaricus campestris and Agaricus blazei using white loam soil as filter layer |
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| CN103947453A (en) * | 2014-04-24 | 2014-07-30 | 江苏农林职业技术学院 | Agaricus bisporus high-quality kernel cultivated species manufacturing method |
| CN106676018B (en) * | 2016-12-30 | 2020-07-21 | 刘天库 | Agaricus bisporus strain breeding method suitable for standardized factory |
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| 3种不同覆土材料对双孢蘑菇栽培的影响;赵凤良等;《安徽农业科学》;20061231;第34卷(第18期);第4556页左栏第22行、右栏第9-13行和表4 * |
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| 王永良.蘑菇覆土及覆土后管理技术.《农技服务》.2008,第25卷(第10期),第45页. |
| 草炭土对双孢蘑菇影响研究;王尚堃;《北方园艺》;20051231(第6期);第96-97页 * |
| 蘑菇覆土及覆土后管理技术;王永良;《农技服务》;20081231;第25卷(第10期);第45页 * |
| 蘑菇麦粒菌种制作的技术关键;陈银回等;《中国食用菌》;19931231;第12卷(第1期);第42-43页 * |
| 赵凤良等.3种不同覆土材料对双孢蘑菇栽培的影响.《安徽农业科学》.2006,第34卷(第18期),第4556页左栏第22行、右栏第9-13行和表4. |
| 陈银回等.蘑菇麦粒菌种制作的技术关键.《中国食用菌》.1993,第12卷(第1期),第42-43页. |
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Granted publication date: 20141022 Termination date: 20180109 |