CN103305448B - Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method - Google Patents
Mixed culture bacterial solution for quickly aging new pit of strong aromatic Chinese spirits and maintenance method Download PDFInfo
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- CN103305448B CN103305448B CN201310278151.9A CN201310278151A CN103305448B CN 103305448 B CN103305448 B CN 103305448B CN 201310278151 A CN201310278151 A CN 201310278151A CN 103305448 B CN103305448 B CN 103305448B
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Abstract
The invention discloses a mixed culture bacterial solution for quickly aging a new pit of strong aromatic Chinese spirits and a maintenance method, and belongs to the field of microorganisms. The mixed culture bacterial solution mainly contains 5-20 parts by weight of methanobacterium culture solution, 1-7 parts by weight of caproic acid bacterium culture solution and 15-30 parts by weight of liquid old pit mud; and the mixed culture bacterial solution is prepared through the following steps of: putting maternal draff, yellow water, medium-high temperature yeast for making hard liquor, yeast extract, sodium acetate and dipotassium phosphate into a fermentation container, performing heat treatment by using boiling water and closing the fermentation container; when the temperature in the fermentation container drops to 40 DEG C, adding the methanobacterium culture solution, the caproic acid bacterium culture solution and the liquid old pit mud as well as an alcohol to the container, full filling the fermentation container with cool boiled water and regulating the pH value to 6.5 to 7.0, and then cultivating at 33 to 37 DEG C. The mixed culture bacterial solution provided by the invention is capable of aging the pit mud more quickly and more efficiently; and therefore, a new method for improving the quality of Chinese spirits is provided.
Description
Technical field
The invention belongs to microorganism field, be specifically related to a kind of aromatic Chinese spirit that makes and newly store mixed culture bacterium liquid aging fast and maintenance process.
Background technology
Aromatic Chinese spirit is one of three large typical aromatic white spirits, and the mud cellar for storing things zymotechnique of its uniqueness creates distinctive local flavor.One of its flavor characteristic of giving off a strong fragrance white wine is that cellar for storing things is aromatic strongly fragrant, and cellar for storing things mud microorganism stores the main source of Studies of The Aromatic Substances just in brewing process.Constantly carrying out along with fermenting process in giving off a strong fragrance liquor production, cellar for storing things mud microorganism species kind and quantity constantly tend to balance with stable, finally reach its specific microbial bacteria group structure, and cellar for storing things mud reaches aging, thus use wine quality and tend towards stability, make the wine aromatic strongly fragrant sense in rich cellar for storing things more.
Luzhou-flavor liquor pit mud quality good or not decides and produces wine quality, therefore the aging particularly important of wine quality acceleration cellar for storing things mud for improving.Because traditional giving off a strong fragrance cellar for storing things mud natural aging is the time needing many decades, but cellar for storing things mud microbe species is various and metabolism is particularly complicated, therefore aging mechanism still cannot be illustrated at present completely.
Giving off a strong fragrance white spirit pit mud aging, basic reason is the continuous training of functional microorganism, Bacterial community is coordinated mutually, finally sets up the process of flora advantage.Cellar for storing things mud, in long-term domestication process, is enriched the various functional microorganisms that a large amount of flavouring is relevant, the microorganisms such as such as caproic acid bacteria, methagen, butyric bacteria, actinomycetes gradually.
The present invention is by Manual propagation cellar for storing things mud functional microorganism, improves new cellar for storing things mud microbial bacteria group structure, achieves aging fast.For mixed culture bacterium liquid of the present invention and foster cellar for storing things method, have not yet to see research report related to this.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of aromatic Chinese spirit that makes and newly stores mixed culture bacterium liquid aging fast and maintenance process.
The technical solution adopted for the present invention to solve the technical problems is: make aromatic Chinese spirit newly store mixed culture bacterium liquid aging fast, comprising: the liquid state of the methagen nutrient solution of 5 ~ 20 weight parts, the caproic acid bacteria nutrient solution of 1 ~ 7 weight part and 15 ~ 30 weight parts stores mud always.
Further, above-mentioned mixed culture bacterium liquid comprises: the liquid state of the methagen nutrient solution of 10 ~ 14 weight parts, the caproic acid bacteria nutrient solution of 3 ~ 5 weight parts and 18 ~ 25 weight parts stores mud always.
Wherein, above-mentioned mixed culture bacterium liquid is obtained by following methods: the dipotassium hydrogen phosphate of the high temperature Daqu of the yellow water of the mother of 3 ~ 5 weight parts grain, 3 ~ 5 weight parts, 2 ~ 4 weight parts, the yeast extract paste of 0.05 ~ 0.2 weight part, the sodium-acetate of 0.1 ~ 0.3 weight part and 0.02 ~ 0.03 weight part is put into fermenting container, then the boiling water adding 30 ~ 50 weight parts carries out sterilising treatment and closes fermenting container; When the temperature in fermenting container is down to 40 DEG C, the alcohol of methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old cellar for storing things mud and 1 ~ 3 weight part is added in container, fill up fermenting container with cold boiling water again and adjust ph to 6.5 ~ 7.0, at the temperature of 33 ~ 37 DEG C cultivate 20 ~ 30 days.
Wherein, in above-mentioned mixed culture bacterium liquid, described methagen nutrient solution adds by cultivating to artificial eye-liquid in the methagen liquid that filters out the bacterium liquid that yeast extract paste cultivates more than 30 days at 33 ~ 37 DEG C of temperature.
Further, the weight of the yeast extract paste of interpolation is 0.5 ~ 1.5 ‰ of methagen liquid weight.
Wherein, in above-mentioned mixed culture bacterium liquid, described caproic acid bacteria nutrient solution adds yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating to artificial eye-liquid in the caproic acid bacterial classification that filters out, then adjust ph is to 6.5 ~ 7.0, the liquid Anaerobic culturel bacterium liquid of 7 ~ 10 days under 35 DEG C of constant temperatures.
Further, with the weight of caproic acid bacterial classification for benchmark, the weight of adding yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of alcohol is 1 ~ 3%, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
Wherein, in above-mentioned mixed culture bacterium liquid, it is after being mixed by the weight ratio of 1 ︰ 0.8 ~ 1 with the cold boiling water of 38 ~ 42 DEG C by the old cellar for storing things mud of maturation that described liquid state stores mud always, add yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing again, load fermenting container and with alcohol to vessel port and liquid level sterilization, at the temperature of 33 ~ 37 DEG C, cultivate the bacterium liquid of 3 ~ 5 days.
Further, always to store the gross weight of mud and cold boiling water for benchmark, the weight of adding yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
The present invention also provides and makes aromatic Chinese spirit newly store maintenance process aging fast, at the bottom of the cellar for storing things of degenerate not aging cellar for storing things mud or appearance in pond, cellar for storing things, cellar for storing things mud digs loose, to dig loose cellar for storing things mud weight for benchmark, the above-mentioned mixed culture bacterium liquid adding 5 ~ 15% loose store mud and mix with digging, then puts down deposited for Jiao Chi with the cellar for storing things mud after mixing.
Wherein, every a bite Jiao Chi processes 2 ~ 3 times as stated above.
The invention has the beneficial effects as follows: the inventive method is the beneficial microorganism adopting Manual propagation methagen, caproic acid bacteria, actinomycetes etc. relevant to producing wine, adopt the maintenance of polynary bacterium liquid, Bacterial community is made to reach balance and optimize, thus improve cellar for storing things mud microbial bacteria group structure, realize a kind of technology that cellar for storing things mud is aging fast.The present invention makes aging more quick, the high-level efficiency of cellar for storing things mud, and the raising for white wine quality provides a kind of new method.
Embodiment
Below by embodiment, the present invention is further detailed.
The present invention makes aromatic Chinese spirit newly store mixed culture bacterium liquid aging fast, comprising: the liquid state of the methagen nutrient solution of 5 ~ 20 weight parts, the caproic acid bacteria nutrient solution of 1 ~ 7 weight part and 15 ~ 30 weight parts stores mud always.
Preferably, above-mentioned mixed culture bacterium liquid comprises: the liquid state of the methagen nutrient solution of 10 ~ 14 weight parts, the caproic acid bacteria nutrient solution of 3 ~ 5 weight parts and 18 ~ 25 weight parts stores mud always.
Preferably, in order to make maintenance effect better, above-mentioned mixed culture bacterium liquid is obtained by following methods: the dipotassium hydrogen phosphate of the high temperature Daqu of the yellow water of the mother of 3 ~ 5 weight parts grain, 3 ~ 5 weight parts, 2 ~ 4 weight parts, the yeast extract paste of 0.05 ~ 0.2 weight part, the sodium-acetate of 0.1 ~ 0.3 weight part and 0.02 ~ 0.03 weight part is put into fermenting container, then the boiling water adding 30 ~ 50 weight parts carries out sterilising treatment and closes fermenting container; When the temperature in fermenting container is down to 40 DEG C, the alcohol of methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old cellar for storing things mud and 1 ~ 3 weight part is added in container, fill up fermenting container with cold boiling water again and adjust ph to 6.5 ~ 7.0, at the temperature of 33 ~ 37 DEG C cultivate 20 ~ 30 days.
In the culturing process of above-mentioned mixed bacteria liquid, the effect of each main substance: 1) effect of female grain is for microorganism provides solid-liquid interface, is conducive to the apposition growth of wherein microorganism, simultaneously for cellar for storing things mud microorganism provides certain nutritive substance; 2) Main Function of yellow water is for the high organic acid of mud microorganism growth and the environment of alcoholic strength are stored in simulation, turns out the beneficial microorganism being suitable for storing mud sour environment, also provide certain nutritive substance simultaneously in culturing process; 3) high temperature Daqu and yeast extract paste, its Main Function is exactly for the cultivation of mixed bacteria liquid provides sufficient nutrient material, i.e. carbon source, nitrogenous source, inorganic salt etc.; 4) Main Function of sodium-acetate is, is suppressed the breeding of the miscellaneous bacterias such as acetic bacteria by free acetate ion; 5) dipotassium hydrogen phosphate main shock absorption playing potential of hydrogen in culturing process, namely ensures that mixed bacteria liquid acidity in culturing process keeps relative stability.Most importantly the mixing of these materials above-mentioned, be not only cellar for storing things mud microbial growth and provide the sufficient nutritive substance such as carbon source, nitrogenous source, inorganic salt, nutritive substance is balanced, and under above-mentioned substance comprehensive action, make the mixing microorganisms of turning out well can be applicable to cellar for storing things mud height organic acid environment.
PH value to 6.5 ~ 7.0 act as balance mixing microorganisms cultivation acidity environment, because the optimal pH value of caproic acid bacteria is that about 6.0 (when pH is too high, its pathways metabolism changes, when pH is too low, its growth and breeding is suppressed even dead), the optimal pH of methagen is then neutral meta-alkalescence, comprehensively above-mentioned two kinds of major microorganisms Dual culture thus pH value is adjusted to 6.5 ~ 7.0.Cultivate 20 ~ 30 days at the temperature of 33 ~ 37 DEG C, its effect is that microbe population is fully bred, and microbial bacteria group structure is continued to optimize, and reaches or always store close to maturation the Bacterial community of mud microorganism; Fully to ferment the different assorted smell removed in nutrient solution through the long period simultaneously, make it ensure to produce the pure of wine aroma for during foster cellar for storing things operation.
Preferably, in order to make maintenance effect better, in above-mentioned mixed culture bacterium liquid, described methagen nutrient solution adds by cultivating to artificial eye-liquid in the methagen liquid that filters out the bacterium liquid that yeast extract paste cultivates more than 30 days at 33 ~ 37 DEG C of temperature.
Preferably, the weight of the yeast extract paste of interpolation is 0.5 ~ 1.5 ‰ of methagen liquid weight.
Preferably, in order to make maintenance effect better, in above-mentioned mixed culture bacterium liquid, described caproic acid bacteria nutrient solution adds yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating to artificial eye-liquid in the caproic acid bacterial classification that filters out, adjust ph is to 6.5 ~ 7.0 again, the liquid Anaerobic culturel bacterium liquid of 7 ~ 10 days under 35 DEG C of constant temperatures.
Preferably, with the weight of caproic acid bacterial classification for benchmark, the weight of adding yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of alcohol is 1 ~ 3%, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
Preferably, in order to make maintenance effect better, in above-mentioned mixed culture bacterium liquid, it is after being mixed by the weight ratio of 1 ︰ 0.8 ~ 1 with the cold boiling water of 38 ~ 42 DEG C by the old cellar for storing things mud of maturation that described liquid state stores mud always, add yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing again, load fermenting container and with alcohol to vessel port and liquid level sterilization, at the temperature of 33 ~ 37 DEG C, cultivate the bacterium liquid of 3 ~ 5 days.
Preferably, always to store the gross weight of mud and cold boiling water for benchmark, the weight of adding yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
The present invention also provides and makes aromatic Chinese spirit newly store maintenance process aging fast, at the bottom of the cellar for storing things of degenerate not aging cellar for storing things mud or appearance in pond, cellar for storing things, cellar for storing things mud digs loose, to dig loose cellar for storing things mud weight for benchmark, the above-mentioned mixed culture bacterium liquid adding 5 ~ 15% loose store mud and mix with digging, then puts down deposited for Jiao Chi with the cellar for storing things mud after mixing.
Preferably, every a bite Jiao Chi processes 2 ~ 3 times as stated above.
Below by embodiment, the specific embodiment of the present invention is further described, but not therefore by protection scope of the present invention restriction in one embodiment.
Embodiment one
Select product Quality of Liquors identical and carry out contrast experiment with batch two mouthfuls of Xin Jiao ponds of building, wherein No. 1 Jiao Chi is according to carrying out step process as follows:
1, the preparation of polynary foster cellar for storing things liquid and treatment process:
(1) artificial eye-liquid cultivates the methagen mixed solution filtered out, and adds yeast extract paste 1 ‰ and cultivates the cultivation bacterium liquid namely for methane bacillus nutrient solution of 30 days at 35 DEG C.
(2) caproic acid caproic acid bacterial classification of good performance is produced in artificial eye-liquid cultivation screening, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, the liquid Anaerobic culturel bacterium liquid of 7 days and caproic acid bacteria nutrient solution under 35 DEG C of constant temperatures.
(3) the cold boiling water of ripe quality aged pit mud and 40 DEG C is chosen, according to 1:1 ratio and add the yeast extract paste 1 ‰ of old cellar for storing things mud and cold boiling water gross weight, sodium-acetate 5 ‰, dipotassium hydrogen phosphate 0.25 ‰ stir loose mixing, load fermenting container and sterilize to vessel port and liquid level with alcohol, at 35 DEG C, the cultivation bacterium liquid of 4 days and liquid state of the present invention store mud always.
(4) select high-quality female poor 4 weight parts, high-quality yellow water 4 weight part, high temperature Daqu powder 3 weight part, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, the boiling water above-mentioned article having been added rear use 40 weight part is heat-treated and closed container; Treat that in fermenting container, temperature is down to 40 DEG C, add methagen nutrient solution 12 weight part, caproic acid bacteria nutrient solution 3 weight part, liquid old cellar for storing things mud 20 weight part, 95Vol% alcohol 2 weight part, all the other fill up culture vessel with cold boiling water, adjust pH to cultivate 25 days at 35 DEG C to 6.8, namely obtain mixed culture bacterium liquid.
(5) at the bottom of the cellar for storing things of not aging cellar for storing things mud or appearance in pond, No. 1 cellar for storing things being degenerated, cellar for storing things mud rake digs loose, add ripe mixed culture bacterium liquid 200 kilograms (digging 10% of loose cellar for storing things mud weight) mixing, wait to process cellar for storing things mud is applied smooth for normal production, continuous above-mentioned process 2 rounds.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation to produce, the end, cellar for storing things grain unstrained spirits produces wine quality in table 1 and table 2:
The main physical and chemical index unit of table 1 wine body: mg/100g
The sensory evaluation of table 2 wine body
| Contrast Jiao Chi | Sensory evaluation |
| No. 1 Jiao Chi | Cellar for storing things perfume (or spice) stronger, sweet mellow, fragrance is comparatively coordinated, aftertaste is clean |
| Blank | Cellar for storing things perfume is not obvious, more pure and sweet, wine body is full not, aftertaste bitter taste is more obvious |
Embodiment two
The two Kou Jiao ponds of producing Quality of Liquors close with degree of degeneration are selected to carry out contrast experiment.
1, the preparation of polynary foster cellar for storing things liquid and treatment process:
(1) artificial eye-liquid cultivates the methagen mixed solution filtered out, and adds yeast extract paste 1 ‰ and cultivates the cultivation bacterium liquid of 30 days at 35 DEG C, namely for methane bacillus nutrient solution.
(2) caproic acid caproic acid bacterial classification of good performance is produced in artificial eye-liquid cultivation screening, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, the liquid Anaerobic culturel bacterium liquid of 10 days under 35 DEG C of constant temperatures, i.e. caproic acid bacteria nutrient solution.
(3) the cold boiling water of ripe quality aged pit mud and 40 DEG C is chosen, according to 1:0.8 ratio and add yeast extract paste 1 ‰, sodium-acetate 5 ‰, dipotassium hydrogen phosphate 0.25 ‰ stir loose mixing, load fermenting container and with alcohol, vessel port and liquid level sterilized, the cultivation bacterium liquid of 4 days at 35 DEG C, namely liquid state of the present invention stores mud always.
(4) select high-quality female poor 4 weight parts, high-quality yellow water 4 weight part, high temperature Daqu powder 3 weight part, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, the boiling water above-mentioned article having been added rear use 40 weight part is heat-treated and closed container; Treat that in fermenting container, temperature is down to 40 DEG C, add methagen nutrient solution 12 weight part, caproic acid bacteria nutrient solution 5 weight part, liquid old cellar for storing things mud 20 weight part, 95Vol% alcohol 2 weight part, all the other fill up culture vessel with cold boiling water, adjust pH to cultivate 30 days at 35 DEG C to 6.8, namely obtain mixed culture bacterium liquid.
(5) at the bottom of the cellar for storing things of not aging cellar for storing things mud or occur being degenerated, cellar for storing things mud rake digs loose, adds ripe mixed culture bacterium liquid 250 kilograms (digging 10% of loose cellar for storing things mud weight) mixing, wait to process cellar for storing things mud is applied smooth for normal production, continuous above-mentioned process 3 rounds.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation to produce, the end, cellar for storing things grain unstrained spirits produces wine quality as table 3 and table 4:
The main physical and chemical index unit of table 3 wine body: mg/100g
The sensory evaluation of table 4 wine body
| Contrast Jiao Chi | Sensory evaluation |
| Experiment Jiao Chi | Cellar for storing things perfume (or spice) comparatively obviously, more mellow, the smell coordination of wine body, aftertaste be clean |
| Contrast Jiao Chi | Cellar for storing things perfume is not obvious, deficient pure and sweet, wine body is thin, aftertaste bitter taste is obvious |
Embodiment three
Select Xin Jiao pond and ripe Lao Jiao pond respectively to carry out contrast experiment flatly, Xin Jiao pond adopts the inventive method process.
1, the preparation of polynary foster cellar for storing things liquid and treatment process:
(1) artificial eye-liquid cultivates the methagen mixed solution filtered out, and adds yeast extract paste 1 ‰ and cultivates the cultivation bacterium liquid of 30 days at 35 DEG C, namely for methane bacillus nutrient solution.
(2) caproic acid caproic acid bacterial classification of good performance is produced in artificial eye-liquid cultivation screening, add yeast extract paste 1 ‰, sodium-acetate 5 ‰, 95Vol% alcohol 2%, dipotassium hydrogen phosphate 0.25 ‰, pH is adjusted to 6.8, the liquid Anaerobic culturel bacterium liquid of 10 days under 35 DEG C of constant temperatures, i.e. caproic acid bacteria nutrient solution.
(3) the cold boiling water of ripe quality aged pit mud and 40 DEG C is chosen, according to 1:1 ratio and add yeast extract paste 1 ‰, sodium-acetate 5 ‰, dipotassium hydrogen phosphate 0.25 ‰ stir loose mixing, load fermenting container and with alcohol, vessel port and liquid level sterilized, the cultivation bacterium liquid of 5 days at 35 DEG C, namely liquid state of the present invention stores mud always.
(4) select high-quality female poor 4 weight parts, high-quality yellow water 4 weight part, high temperature Daqu powder 3 weight part, yeast extract paste 0.1 weight part, sodium-acetate 0.2 weight part, dipotassium hydrogen phosphate 0.025 weight part, the boiling water above-mentioned article having been added rear use 40 weight part carries out heat place and closed container reason; Treat that in fermenting container, temperature is down to 40 DEG C, add methagen nutrient solution 12 weight part, caproic acid bacteria nutrient solution 5 weight part, liquid old cellar for storing things mud 20 weight part, 95Vol% alcohol 2 weight part, all the other fill up culture vessel with cold boiling water, adjust pH to cultivate 30 days at 35 DEG C to 6.8, namely obtain mixed culture bacterium liquid.
(5) at the bottom of the cellar for storing things of not aging cellar for storing things mud or occur being degenerated, cellar for storing things mud rake digs loose, adds ripe mixed culture bacterium liquid 250 kilograms (digging 10% of loose cellar for storing things mud weight) mixing, wait to process cellar for storing things mud is applied smooth for normal production, continuous above-mentioned process 2 rounds.
2, all carry out former cellar for storing things according to identical manufacturing parameter and technological operation to produce, the end, cellar for storing things grain unstrained spirits produces wine quality as table 5 and table 6:
The main physical and chemical index unit of table 5 wine body: mg/100g
The sensory evaluation of table 6 wine body
Analysis through above comparison example is known: example one comparing result shows, for the new cellar for storing things mud of same batch, present method agingly has obvious promoter action to it, is mainly reflected in out wine quality and significantly improves; Example two comparing result shows, utilizes the maintenance that this mixed bacteria liquid carries out, and to occur hardening the phenomenon of degenerating to cellar for storing things mud, tool improves significantly, and also has obvious raising to going out wine quality simultaneously, and main manifestations is take ethyl hexanoate as the increase of main body fragrant content; Example three comparing result shows, utilizes the Xin Jiao pond of this mixed bacteria liquid maintenance process, and it goes out the old of wine quality access expansion maturation and stores out wine quality, therefore verifies that mud is aging has obvious promoter action to the method to storing further.
As known from the above examples: by the maintenance process of this mixed bacteria liquid, the microbial bacteria group structure of cellar for storing things mud is optimized, and cellar for storing things mud Oranoleptic indicator, physical and chemical index and microbiological indicator all improve significantly; Go out wine quality to significantly improve, liquor body style shows as simultaneously: store aromatic strongly fragrant, wine body is coordinated more, mellow.By the application of present method, new cellar for storing things mud can in the effect that the short period reaches or access expansion is aging, and pond, the cellar for storing things tool of simultaneously degenerating for appearance improves significantly.
Claims (7)
1. make aromatic Chinese spirit newly store mixed culture bacterium liquid aging fast, it is characterized in that comprising: the liquid state of the methagen nutrient solution of 5 ~ 20 weight parts, the caproic acid bacteria nutrient solution of 1 ~ 7 weight part and 15 ~ 30 weight parts stores mud always; Described methagen nutrient solution adds by cultivating to artificial eye-liquid in the methagen liquid that filters out the bacterium liquid that yeast extract paste cultivates more than 30 days at 33 ~ 37 DEG C of temperature; Described caproic acid bacteria nutrient solution adds yeast extract paste, sodium-acetate, alcohol and dipotassium hydrogen phosphate by cultivating to artificial eye-liquid in the caproic acid bacterial classification that filters out, then adjust ph is to 6.5 ~ 7.0, the liquid Anaerobic culturel bacterium liquid of 7 ~ 10 days under 35 DEG C of constant temperatures;
Mixed culture bacterium liquid is obtained by following methods: the dipotassium hydrogen phosphate of the high temperature Daqu of the yellow water of the mother of 3 ~ 5 weight parts grain, 3 ~ 5 weight parts, 2 ~ 4 weight parts, the yeast extract paste of 0.05 ~ 0.2 weight part, the sodium-acetate of 0.1 ~ 0.3 weight part and 0.02 ~ 0.03 weight part is put into fermenting container, then the boiling water adding 30 ~ 50 weight parts carries out sterilising treatment and closes fermenting container; When the temperature in fermenting container is down to 40 DEG C, the alcohol of methagen nutrient solution, caproic acid bacteria nutrient solution, liquid old cellar for storing things mud and 1 ~ 3 weight part is added in container, fill up fermenting container with cold boiling water again and adjust ph to 6.5 ~ 7.0, at the temperature of 33 ~ 37 DEG C cultivate 20 ~ 30 days.
2. the aromatic Chinese spirit that makes according to claim 1 newly stores mixed culture bacterium liquid aging fast, it is characterized in that comprising: the liquid state of the methagen nutrient solution of 10 ~ 14 weight parts, the caproic acid bacteria nutrient solution of 3 ~ 5 weight parts and 18 ~ 25 weight parts stores mud always.
3. the aromatic Chinese spirit that makes according to claim 1 and 2 newly stores mixed culture bacterium liquid aging fast, it is characterized in that: the weight of the yeast extract paste of interpolation is 0.5 ~ 1.5 ‰ of methagen liquid weight.
4. the aromatic Chinese spirit that makes according to claim 1 and 2 newly stores mixed culture bacterium liquid aging fast, it is characterized in that: with the weight of caproic acid bacterial classification for benchmark, add that the weight of yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of alcohol is 1 ~ 3%, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
5. the aromatic Chinese spirit that makes according to claim 1 and 2 newly stores mixed culture bacterium liquid aging fast, it is characterized in that: it is after being mixed by the weight ratio of 1 ︰ 0.8 ~ 1 with the cold boiling water of 38 ~ 42 DEG C by the old cellar for storing things mud of maturation that described liquid state stores mud always, add yeast extract paste, sodium-acetate and dipotassium hydrogen phosphate stirring and evenly mixing again, load fermenting container and with alcohol to vessel port and liquid level sterilization, at the temperature of 33 ~ 37 DEG C, cultivate the bacterium liquid of 3 ~ 5 days.
6. the aromatic Chinese spirit that makes according to claim 5 newly stores mixed culture bacterium liquid aging fast, it is characterized in that: always to store the gross weight of mud and cold boiling water for benchmark, add that the weight of yeast extract paste is 0.5 ~ 1.5 ‰, the weight of sodium-acetate is 4 ~ 6 ‰, the weight of dipotassium hydrogen phosphate is 0.2 ~ 0.3 ‰.
7. make aromatic Chinese spirit newly store maintenance process aging fast, it is characterized in that: at the bottom of the cellar for storing things of degenerate not aging cellar for storing things mud or appearance in pond, cellar for storing things, cellar for storing things mud digs loose, to dig loose cellar for storing things mud weight for benchmark, the mixed culture bacterium liquid according to any one of claim 1 ~ 6 adding 5 ~ 15% loose store mud and mix with digging, then puts down deposited for Jiao Chi with the cellar for storing things mud after mixing.
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