CN103305443A - Preparation and method for restoring ammonia nitrogen-containing industrial sewage - Google Patents
Preparation and method for restoring ammonia nitrogen-containing industrial sewage Download PDFInfo
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- CN103305443A CN103305443A CN2013102413682A CN201310241368A CN103305443A CN 103305443 A CN103305443 A CN 103305443A CN 2013102413682 A CN2013102413682 A CN 2013102413682A CN 201310241368 A CN201310241368 A CN 201310241368A CN 103305443 A CN103305443 A CN 103305443A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 239000010865 sewage Substances 0.000 title claims abstract description 25
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims description 30
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 12
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 12
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 12
- 241000186074 Arthrobacter globiformis Species 0.000 claims abstract description 11
- 241001509286 Thiobacillus denitrificans Species 0.000 claims abstract description 11
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 3
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- WWUZIQQURGPMPG-UHFFFAOYSA-N (-)-D-erythro-Sphingosine Natural products CCCCCCCCCCCCCC=CC(O)C(N)CO WWUZIQQURGPMPG-UHFFFAOYSA-N 0.000 claims description 9
- WWUZIQQURGPMPG-KRWOKUGFSA-N sphingosine Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)CO WWUZIQQURGPMPG-KRWOKUGFSA-N 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 6
- 241001103617 Pseudomonas aeruginosa ATCC 15442 Species 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 241001135759 Sphingomonas sp. Species 0.000 claims description 2
- 241000736131 Sphingomonas Species 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 9
- 239000002351 wastewater Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 230000031018 biological processes and functions Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 238000005660 chlorination reaction Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 239000008176 lyophilized powder Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000009388 chemical precipitation Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- -1 olefines Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical class [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
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- 239000002671 adjuvant Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical group 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000007233 catalytic pyrolysis Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
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- 229930195733 hydrocarbon Natural products 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
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- 239000010802 sludge Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a preparation for restoring ammonia nitrogen-containing industrial sewage. The preparation is prepared from the following components: 100 parts of thiobacillus denitrificans, 80 parts of bacillus licheniformis, 60 parts of sphingomonas, 50 parts of phanerochaete chrysosporium, 40 parts of arthrobacter globiformis, 30 parts of alcaligenes faecalis, 10 parts of pseudomonas aeruginosa and 10 parts of bacillus cereus. The invention also discloses a preparation method of the preparation. The preparation disclosed by the invention is suitable for the treatment of high-ammonia nitrogen industrial sewage, is energy-saving and environment-friendly, and has better application prospects.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to preparation and method that a kind of reparation contains the ammonia nitrogen industrial sewage.
Background technology
The industrial pollution water body refers to the waste water, sewage and the waste liquid that produce in the Industrial processes, wherein contains the pollutent that produces in the industrial production materials, intermediate product and the product that outflow with water and the production process.Along with developing rapidly of industry, kind and the quantity of waste water rapidly increase, and is also increasingly extensive and serious to the pollution of water body, threatens human health and safety.Therefore, for protection of the environment, the processing of trade effluent is more even more important than the processing of municipal effluent.
The composition of industrial sewage is more complicated, a large amount of artificial-synthetic compound's entered environment particularly, this class material mainly is alkanes, the ammonia nitrogen class, olefines, alicyclic hydrocarbon type, arene, phosphide etc., because the complicacy of these these body structures of material and biological strange property, can not be decomposed by microorganism at short notice and utilize, traditional method of wastewater treatment can not be removed these pollutents effectively with the microorganism of activated sludge culture, these materials accumulate in environment for a long time, the ecotope of depending on for existence to us causes very large pollution, brings very large harm for human physical and mental health.Quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer waste water, even there is the waste disposal plant operation also abnormal.Therefore, develop that a kind of construction investment is few, running cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
According to the difference of ammonia nitrogen in sewage concentration, waste water can be divided into 3 classes: high-concentration ammonia nitrogenous wastewater (NH3-N＞500mg/l), intermediate concentration ammonia nitrogen waste water (NH3-N:50-500mg/l), Low Concentration Ammonia Containing Wastewater (NH3-N＜50mg/l).Yet the ammonia nitrogen waste water of high density has restraining effect to microbic activity, has restricted processing application and the effect of biochemical process to it, can reduce biochemical system to the degradation efficiency of organic pollutant simultaneously, is difficult to reach requirement thereby cause processing water outlet.In the prior art there be the main method of removal ammonia nitrogen commonly used: Physical, chemical method, biological process.Physical contains the treatment technologies such as reverse osmosis, distillation, soil irrigation; Chemical method contains the treatment technologies such as ion-exchange, ammonia stripping, break point chlorination, burning, chemical precipitation, catalytic pyrolysis, electrodialysis, electrochemistry; Biological process contains the treatment technologies such as algal culture, biological nitration, immobilized biotechnology.Practical method has at present: break point chlorination method, selective ion exchange method, ammonia blow-off method, biological process and chemical precipitation method.Wherein, break point chlorination method, selective ion exchange method, ammonia blow-off method are not suitable for large-scale industrialization and use, and chemical process easily causes other pollutions, and cost is higher, and the method for at present the most economic environmental protection is biological process.
Summary of the invention
The purpose of this invention is to provide a kind of reparation contains the preparation of ammonia nitrogen industrial sewage and utilizes the dispose of sewage method of ammonia nitrogen of said preparation.
Sewage disposal composite fungus agent of the present invention, reasonable compatibility between each bacterial classification, symbiosis is coordinated, mutual antagonism not, its preparation method is easy, and is easy to implement the method, and it is easy and simple to handle, is beneficial to suitability for industrialized production.
The present invention adopts following technical scheme to realize: a kind of reparation contains the preparation of ammonia nitrogen industrial sewage, and the activeconstituents of said preparation comprises the raw material bacterium of following weight part:
100 parts of thiobacillus denitrificans, 80 parts of Bacillus licheniformis, 60 parts of sphingosine sporangiums, 50 parts of Phanerochaete chrysosporiums, 40 parts of Arthrobacter globiformis, 30 parts of Bacillus foecalis alkaligeness, 10 parts of pseudomonas aeruginosas, 10 parts of bacillus cereuses.
1) described thiobacillus denitrificans (Thiobacillus denitrificans) specifically can be preferred ATCC25259 (reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY for example, May2007, p.3265-3271)
2) described Bacillus licheniformis (Bacillus licheniformis) specifically can be preferably CCTCC NO.M206082 (CN101037659A)
3) described sphingosine sporangium (Sphingomonas sp.) is preferably CGMCC NO.4589 (CN102168054A);
4) the preferred ATCC24725 of described Phanerochaete chrysosporium (Phanerochaete chrysosporium) (for example APPLIEDAND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714);
5) described Arthrobacter globiformis (Arthrobacter globiformi s) is preferably CCTCC NO:M209015 (CN101492649A)
6) described Bacillus foecalis alkaligenes (Alcaligenes faecalis) is preferably Bacillus foecalis alkaligenes ATCC 31555 (for example referring to document Structural studies of an extracellular polysaccharide (S-130) elaborated by AlcaligenesATCC31555, Carbohydrate Research, 1986);
7) described pseudomonas aeruginosa (Pseudomonas aeruginosa) specifically can be pseudomonas aeruginosa ATCC 15442 (for example referring to document Adaptation of Pseudomonas aeruginosa ATCC 15442 todidecyldimethylammonium bromide induces changes in membrane fatty acid composition and in resistance of cells, Journal of Applied Microbiology, 2001);
8) described bacillus cereus (Bacillus cereus) specifically can be ATCC10876 (New J.Chem. for example, 2007,31,748-755).
Bacterial classification of the present invention all can obtain from CGMCC, CCTCC and US mode culture collection warehousing (ATCC) purchase.
What the existence form of composite fungus agent of the present invention was better is the mixture of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material.When the existence form of described microbial inoculum was lyophilized powder, the preparation method comprised above-mentioned bacterium is prepared respectively fermented liquid, after mixing according to mass ratio, makes lyophilized powder by this area ordinary method and gets final product.
Wherein each strain fermentating liquid preparation process is preferably in the following manner:
The independent enlarged culturing of various bacterial classifications:
The training method of thiobacillus denitrificans: be inoculated into (Na on the substratum
2S
2O
35H
2O 5g, KNO
32g, KH
2PO
42g, NaHCO
31g, MgCl
26H2O0.5g, FeSO47H2O0.01g NH
4Cl0.5g, water 1000ml), 28-30 ℃, make primary inclined plane and cultivate, then secondary seed is cultivated, mixing fermentation culture viable count to the product reaches 1 * 10
8Individual/gram.
The cultivation of Bacillus licheniformis or bacillus cereus: at first be seeded in withered Bacillus licheniformis or bacillus cereus test tube kind on the beef-protein medium, 28-30 ℃, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, then change liquid fermentation tank over to and do three grades of liquid culture, be inoculated at last and make level Four on the solid medium and cultivate, viable count reaches 1 * 10 to the product
8Individual/gram.
The training method of pseudomonas aeruginosa or Bacillus foecalis alkaligenes: pseudomonas aeruginosa or Bacillus foecalis alkaligenes at first on substratum, 28-30 ℃, make primary inclined plane and cultivate, then secondary seed is cultivated, mixing fermentation culture viable count to the product reaches 1.0 * 10
8Individual/gram, described medium component is: NH
4Cl 1.0g, CH
3COONa 3.5g, MgCl
20.1g, CaCl
20.1g, KH
2PO
40.6g, K
2HPO
40.4g, yeast extract paste 0.1g, water 1000ml, pH7.2.
The training method of sphingosine sporangium: the sphingosine sporangium at first is seeded on the ox yeast extract paste protein culture medium substratum, 28-30 ℃, do then that level liquid seed culture, secondary liquid seeds are cultivated and fermentor cultivation viable count to the product reaches 1.0 * 10
8Individual/gram.
The training method of Phanerochaete chrysosporium or Arthrobacter globiformis: Phanerochaete chrysosporium at first is inoculated on the potato dextrose agar, 28-30 ℃, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, then change liquid fermentation tank over to and do three grades of liquid culture, be inoculated at last and make level Four on the solid medium and cultivate, viable count reaches 1 * 10 to the product
8Individual/gram.
The bacterium of above-mentioned cultivation is mixed by described weight part, then carry out cryodrying, drying temperature is 20-30 ℃, and dry rear water content is 20%; Pack at last and get final product.
Annotate, strain expanded culture and the method for preparing solid fungicide are not unique in the above-mentioned steps, and those skilled in the art can select suitable medium and enlarged culturing method according to general knowledge, make viable count reach 10
8Individual/gram, and the method preparation for preparing solid fungicide according to routine.
Composite fungus agent of the present invention is with the various bacterial classifications that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be added in the Waste Water Treatment that is rich in ammonia nitrogen by a certain amount of, accelerate the degraded of microbe, to improve the biological treatment efficiency of system, guarantee system stable operation.Its pollutent that contains ammonia nitrogen has the microorganism of good degradation capability, reasonable compatibility between each bacterial classification, and symbiosis is coordinated, mutual antagonism not, active high, biomass is large, and breeding is fast.Composite fungus agent of the present invention is suitable for industrial sewage to be processed, and can improve and process the water yield and water quality treatment, reduces working cost, promotes qualified discharge.
Embodiment
The present invention is further elaborated below will to adopt the mode of specific embodiment, but it should not be construed the restriction to core initiative spirit of the present invention.
Embodiment 1
A kind of reparation contains the preparation of ammonia nitrogen industrial sewage, and said preparation is prepared from by the raw material bacterium of following weight part:
100 parts of thiobacillus denitrificans, 80 parts of Bacillus licheniformis, 60 parts of sphingosine sporangiums, 50 parts of Phanerochaete chrysosporiums, 40 parts of Arthrobacter globiformis, 30 parts of Bacillus foecalis alkaligeness, 10 parts of pseudomonas aeruginosas, 10 parts of bacillus cereuses.
The preparation process of preparation: the raw material bacterium of cultivating is mixed by weight, then carry out cryodrying, drying temperature is 20-30 ℃, and dry rear water content is 20%; Pack at last and get final product.
Industrial application: (NH3-N＞500mg/L), at every turn add preparation 10 grams by every cubic metre of sewage adds 1 every day, adds continuously 10 days for the treatment of the ammonia nitrogen in high density industrial sewage with said preparation; After one month, the clearance of the ammonia nitrogen of sewage can reach more than 99%.
Embodiment 2
A kind of reparation contains the preparation of ammonia nitrogen industrial sewage, and the activeconstituents of said preparation comprises the raw material bacterium of following weight part:
100 parts of thiobacillus denitrificans, 80 parts of Bacillus licheniformis, 60 parts of sphingosine sporangiums, 50 parts of Phanerochaete chrysosporiums, 40 parts of Arthrobacter globiformis, 30 parts of Bacillus foecalis alkaligeness, 10 parts of pseudomonas aeruginosas, 10 parts of bacillus cereuses;
Wherein, thiobacillus denitrificans is ATCC25259, Bacillus licheniformis is CCTCC NO.M206082, the sphingosine sporangium is CGMCC NO.4589, Phanerochaete chrysosporium is ATCC 24725, and Arthrobacter globiformis is CCTCC NO:M209015, and Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes ATCC 31555, pseudomonas aeruginosa is ATCC 15442, and bacillus cereus is ATCC 10876.
The preparation process of preparation: the raw material bacterium of cultivating is mixed by weight, then carry out cryodrying, drying temperature is 20-30 ℃, and dry rear water content is 20%; Pack at last and get final product.
Industrial application: (NH3-N＞500mg/L), at every turn add preparation 10 grams by every cubic metre of sewage adds 1 every day, adds continuously 10 days for the treatment of the ammonia nitrogen in high density industrial sewage of Linyi City Linshu County fertilizer plant with said preparation; After one month, said preparation possesses the effect of better removal ammonia nitrogen, treatment effect such as following table 1:
Table 1
Although, above with general explanation and embodiment detailed explanation having been done in this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the modification of doing without departing from theon the basis of the spirit of the present invention or improvement all belong to the scope of protection of present invention.
Claims (4)
1. a reparation contains the preparation of ammonia nitrogen industrial sewage, and said preparation is prepared from by the raw material bacterium of following weight part:
100 parts of thiobacillus denitrificans, 80 parts of Bacillus licheniformis, 60 parts of sphingosine sporangiums, 50 parts of Phanerochaete chrysosporiums, 40 parts of Arthrobacter globiformis, 30 parts of Bacillus foecalis alkaligeness, 10 parts of pseudomonas aeruginosas, 10 parts of bacillus cereuses.
2. preparation as claimed in claim 1, it is characterized in that, described thiobacillus denitrificans (Thiobacillus denitrificans) can be ATCC25259, described Bacillus licheniformis (Bacillus licheniformis) can be CCTCC NO.M206082, described sphingosine sporangium (Sphingomonas sp.) can be CGMCC NO.4589, described Phanerochaete chrysosporium (Phanerochaete chrysosporium) but be ATCC 24725, described Arthrobacter globiformis (Arthrobacter globiformis) can be CCTCC NO:M209015, described Bacillus foecalis alkaligenes (Alcaligenes faecalis) can be Bacillus foecalis alkaligenes ATCC 31555, described pseudomonas aeruginosa (Pseudomonas aeruginosa) can be pseudomonas aeruginosa ATCC 15442, and described bacillus cereus (Bacillus cereus) can be ATCC 10876.
3. a reparation contains the method for ammonia nitrogen industrial sewage, it is characterized in that, described method has been used claim 1 or 2 described preparations.
4. method as claimed in claim 3 is characterized in that, the preparation of claim 1 or 2 is processed the ammonia nitrogen in high density industrial sewage, and every cubic metre of sewage adds about preparation 10 grams at every turn, adds every day 1 time, adds continuously 10 days.
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| CN108034624A (en) * | 2018-02-05 | 2018-05-15 | 厦门理工学院 | It is a kind of to be used to handle biological agent of high-concentration ammonia nitrogenous wastewater and preparation method thereof |
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| CN104059864A (en) * | 2014-05-26 | 2014-09-24 | 绍兴柯桥江滨水处理有限公司 | Bacilluscereus as well as preparation method and application thereof |
| CN107099483A (en) * | 2017-06-26 | 2017-08-29 | 金文忠 | A kind of composite biological agent and its application in processing mercury-containing waste water |
| CN107099483B (en) * | 2017-06-26 | 2020-09-18 | 杭州富阳佳畅机械有限公司 | Composite biological agent and application thereof in treatment of mercury-containing wastewater |
| CN108034624A (en) * | 2018-02-05 | 2018-05-15 | 厦门理工学院 | It is a kind of to be used to handle biological agent of high-concentration ammonia nitrogenous wastewater and preparation method thereof |
| CN110386676A (en) * | 2019-08-12 | 2019-10-29 | 东莞市台腾环保材料科技有限公司 | A kind of double tank up flow type biological filter tank systems and its method for handling organic matter |
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