CN103305335B - Low-temperature countercurrent extraction method of euphausia superba sauce - Google Patents
Low-temperature countercurrent extraction method of euphausia superba sauce Download PDFInfo
- Publication number
- CN103305335B CN103305335B CN201310262319.7A CN201310262319A CN103305335B CN 103305335 B CN103305335 B CN 103305335B CN 201310262319 A CN201310262319 A CN 201310262319A CN 103305335 B CN103305335 B CN 103305335B
- Authority
- CN
- China
- Prior art keywords
- extraction
- euphausia superba
- countercurrent extraction
- sauce
- countercurrent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Meat, Egg Or Seafood Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a low-temperature countercurrent extraction method of euphausia superba sauce. The method comprises the steps of I. drying and grinding; II. countercurrent extraction; and III. solvent evaporation. The prepared euphausia superba sauce comprises the following main components by weight percent: 35-40% of phospholipid, 13-18% of EPA (eicosapentaenoic acid) and 8-12% of DHA (docosahexaenoic acid). The method has the beneficial effects that during countercurrent extraction, solvents are utilized to extract the raw materials reversely, form concentration difference in the flowing process, can come into full contact with the raw materials and are beneficial to leaching of active ingredients, and the extraction efficiency is improved; the whole operational process is simple, the quality is stable and the extraction process does not have adverse effects on the environment and is safe and practical; and compared with the prior art, the method has the characteristics of easier raw material treatment, solvent saving, high extraction efficiency, low cost and the like and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of extracting method of shrimp sauce, particularly relate to a kind of method of low temperature countercurrent extraction euphausia superba sauce.
Background technology
Krill (Euphausia superba), has another name called large krill or Antarctic krill, is the krill of one way of life in waters, the Antarctica.Krill lives in marine site, less than the 0 DEG C free of contamination South Pole for a long time, with plant plankton (algae) for food.Research finds, krill is one of biology that current global protein content is the highest, and weight in wet base reaches 17% containing albumen, and dry weight reaches 70%, is called as in " the animal proteinum storehouse of human future "; Also be rich in shrimp sauce, astaxanthin, cold-adapted enzyme isoreactivity material in its body, there is wide application prospect, comprehensive exploitation Huge value in fields such as medicine, chemical industry, agricultural, aquatic products.
The lipid content of krill accounts for the 1-6% of weight in wet base, main types of fats comprises: Yelkin TTS (PC) (33-36%), phosphatidylethanolamine (PE) (5-6%), triglyceride level (TG) (33-40%), free fatty acids (FFA) (8-16%), astaxanthin etc.Wherein, krill contains the phosphatide that abundant polyunsaturated fatty acid (DHA and EPA) forms, the phosphatide of polyunsaturated fatty acid composition plays an important role in human body and animal body, there is raising memory, prevention senile dementia, effect such as prevention cardiovascular and cerebrovascular diseases and atherosclerosis etc.So it is increasingly important to extract its phosphatide technology be rich in from the grease of krill.
Method mainly organic solvent extraction and the super critical extraction of current extraction euphausia superba sauce.Organic solvent extraction many employings normal hexane, acetone, ethyl acetate etc. are extracted, and extraction yield is higher, but residual solvent is difficult to remove completely, and also there is environment and safety problem in production process.And super critical extraction extraction yield is lower, the phospholipids content extracted is also lower, and adopt supercritical carbon dioxide extraction euphausia superba sauce, can improve extraction yield, but it is high to there is working pressure, equipment manufacturing cost is high, is difficult to shortcomings such as applying.
Summary of the invention
The present invention, in order to overcome the above-mentioned shortcoming existed in prior art, provides a kind of method of low temperature countercurrent extraction euphausia superba sauce.
For achieving the above object, the technology used in the present invention solution is: a kind of method of low temperature countercurrent extraction euphausia superba sauce, and its step comprises:
One, drying and crushing: krill is carried out lyophilize or heat dry dry after, then through pulverize, grinding after cross 60 mesh sieves, obtain the euphausia superba powder of water content≤10%;
Two, countercurrent extraction: add krill meal in countercurrent extractor, adds material quantity 3 ~ 5 times, concentration is the ethanol of 95%, and temperature controls at 30-50 DEG C of countercurrent extraction 60min, extracts three times, united extraction liquid;
Three, solvent evaporates: by the extracting solution of merging temperature 35 ~ 55 DEG C, evaporate under vacuum tightness-0.06 ~-0.085MPa, remove the organic solvent in extracting solution, obtain the euphausia superba sauce that phospholipids content is 35 ~ 40%; Then removing residual organic solvent in shrimp sauce by leading to nitrogen under euphausia superba sauce normal temperature continuously, making the residual quantity of organic solvent meet national standard, obtaining euphausia superba sauce.
Further, the euphausia superba sauce main component prepared in described step 3 and content (by weight) thereof: phosphatide: 35 ~ 40%, EPA: 13 ~ 18%, DHA: 8 ~ 12%.
The invention has the beneficial effects as follows: countercurrent extraction uses that solvent is reverse extracts raw material, and solvent forms concentration difference in flow process, and fully can contact with raw material, is conducive to the leaching of effective constituent, improves extraction efficiency.Whole operating process is simple, and steady quality, leaching process has no adverse effects to environment, safe and practical.There is Feedstock treating compared with art methods be more prone to, save the features such as solvent, extraction efficiency is high, cost is low, be suitable for suitability for industrialized production.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
embodiment 1
A method for low temperature countercurrent extraction antarctic krill oil, the steps include:
(1) drying and crushing: krill is carried out lyophilize or heat dry dry after, then through pulverize, grinding after cross 60 mesh sieves, obtain the euphausia superba powder that water content is 8%;
(2) countercurrent extraction: add krill meal 400g in countercurrent extractor, adds 2000ml, concentration is the ethanol of 95%, and temperature controls at 30 DEG C of countercurrent extraction 60min, extracts three times, united extraction liquid;
(3) solvent evaporates: by the extracting solution of merging temperature 35 DEG C, evaporate, remove the organic solvent in extracting solution under vacuum tightness-0.06MPa, obtains red euphausia superba sauce; Remove residual organic solvent in shrimp sauce by leading to nitrogen under antarctic krill oil normal temperature continuously, obtain antarctic krill oil 63.8g, yield is 15.9%.
Antarctic krill oil main component and content as follows: phosphatide: 37.8 %, EPA: 16.9%, DHA: 11.4%.
embodiment 2
A method for low temperature countercurrent extraction antarctic krill oil, the steps include:
(1) drying and crushing: krill is carried out lyophilize or heat dry dry after, then through pulverize, grinding after cross 60 mesh sieves, obtain the euphausia superba powder of water content 10%;
(2) countercurrent extraction: add krill meal 400g in countercurrent extractor, adds 2400ml, concentration is the ethanol of 95%, and temperature controls at 40 DEG C of countercurrent extraction 60min, extracts three times, united extraction liquid;
(3) solvent evaporates: by the extracting solution of merging temperature 40 DEG C, evaporate, remove the organic solvent in extracting solution under vacuum tightness-0.07MPa, obtains red antarctic krill oil; Fall residual organic solvent in shrimp sauce by leading to nitrogen under antarctic krill oil normal temperature continuously, obtain antarctic krill oil 62.3g, yield is 15.6%.
Antarctic krill oil main component and content as follows: phosphatide: 37.5 %, EPA: 17.6%, DHA: 11.8%.
embodiment 3
A method for low temperature countercurrent extraction antarctic krill oil, the steps include:
(1) drying and crushing: krill is carried out lyophilize or heat dry dry after, then through pulverize, grinding after cross 60 mesh sieves, obtain the euphausia superba powder of water content 9%;
(2) countercurrent extraction: add krill meal 400g in countercurrent extractor, adds 2800ml, concentration is the ethanol of 95%, and temperature controls at 50 DEG C of countercurrent extraction 60min, extracts three times, united extraction liquid;
(3) solvent evaporates: by the extracting solution of merging at temperature 50 C, evaporate, remove the organic solvent in extracting solution under vacuum tightness-0.08MPa, obtains red euphausia superba sauce; Remove residual organic solvent in shrimp sauce by leading to nitrogen under euphausia superba sauce normal temperature continuously, obtain antarctic krill oil 65.4g, yield is 16.4%.
Antarctic krill oil main component and content as follows: phosphatide: 38.8%, EPA: 18.1%, DHA: 12.2%.
As can be seen from above-described embodiment, the present invention has following characteristics:
1, use ethanol to carry out the extraction of krill shrimp sauce as extraction solvent, the degree of krill nutritive ingredient sex change can be reduced, be conducive to the later use of krill meal.
2, countercurrent extraction uses that solvent is reverse extracts raw material, solvent forms concentration difference in flow process, and fully can contact with raw material, be conducive to the leaching of effective constituent, well-beaten effect is played in countercurrent flow simultaneously in leaching process, further raising extraction efficiency, can obtain the antarctic krill oil of high yield.
3, low temperature adverse current prepares antarctic krill oil, and extract and shorten extraction time, decrease solvent usage quantity, simple to operate, labour intensity is low, and extraction yield is high, significantly can reduce production cost.
4, the method flow process is simple, steady quality, leaching process on environment without impact, safe and practical.
Countercurrent extraction uses that solvent is reverse extracts raw material, and solvent forms concentration difference in flow process, and fully can contact with raw material, is conducive to the leaching of effective constituent, improves extraction efficiency.Whole operating process is simple, and steady quality, leaching process has no adverse effects to environment, safe and practical.There is Feedstock treating compared with art methods be more prone to, save the features such as solvent, extraction efficiency is high, cost is low, be suitable for suitability for industrialized production.
The above, be only the specific embodiment of the present invention, is not limited thereto, and is anyly familiar with those skilled in the art in the technical scope that this patent discloses, and can expect change easily or replace, all should be encompassed within protection scope of the present invention.
Claims (1)
1. a method for low temperature countercurrent extraction antarctic krill oil, the steps include:
(1) drying and crushing: krill is carried out lyophilize or heat dry dry after, then through pulverize, grinding after cross 60 mesh sieves, obtain the euphausia superba powder of water content 9%;
(2) countercurrent extraction: add krill meal 400g in countercurrent extractor, adds 2800ml, concentration is the ethanol of 95%, and temperature controls at 50 DEG C of countercurrent extraction 60min, extracts three times, united extraction liquid;
(3) solvent evaporates: by the extracting solution of merging at temperature 50 C, evaporate, remove the organic solvent in extracting solution under vacuum tightness-0.08MPa, obtains red euphausia superba sauce; Remove residual organic solvent in shrimp sauce by leading to nitrogen under euphausia superba sauce normal temperature continuously, obtain antarctic krill oil 65.4g, yield is 16.4%;
Antarctic krill oil main component and content as follows: phosphatide: 38.8%, EPA:18.1%, DHA:12.2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310262319.7A CN103305335B (en) | 2013-06-27 | 2013-06-27 | Low-temperature countercurrent extraction method of euphausia superba sauce |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310262319.7A CN103305335B (en) | 2013-06-27 | 2013-06-27 | Low-temperature countercurrent extraction method of euphausia superba sauce |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103305335A CN103305335A (en) | 2013-09-18 |
| CN103305335B true CN103305335B (en) | 2015-04-29 |
Family
ID=49131059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310262319.7A Expired - Fee Related CN103305335B (en) | 2013-06-27 | 2013-06-27 | Low-temperature countercurrent extraction method of euphausia superba sauce |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103305335B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021194360A1 (en) * | 2020-03-27 | 2021-09-30 | Pharmazen Limited | Extraction method for bio-active fractions |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694244B (en) * | 2013-12-06 | 2017-12-08 | 青岛康境海洋生物科技有限公司 | A kind of krill oil preparation method of the high esterification of astaxanthin high phospholipid of duo-sol extraction |
| CN103981021A (en) * | 2014-05-31 | 2014-08-13 | 山东乾清翔泰生物制品有限公司 | Method for refining krill oil from Antarctic krill powder |
| CN104830525B (en) * | 2015-05-12 | 2018-06-05 | 大连工业大学 | A kind of multi-stage countercurrent of antarctic krill oil continuously leaches and process for purification |
| CN108004016A (en) * | 2017-12-18 | 2018-05-08 | 青岛康境海洋生物科技有限公司 | A kind of preparation method of the antarctic krill oil of high lecithin content |
| CN108130193B (en) * | 2017-12-21 | 2021-04-02 | 山东师范大学 | Method for improving extraction rate of oil from euphausia superba |
| CN109439430B (en) * | 2018-10-22 | 2021-10-08 | 辽渔南极磷虾科技发展有限公司 | Euphausia superba oil refining method |
| CN109198042B (en) * | 2018-10-22 | 2021-10-08 | 辽渔南极磷虾科技发展有限公司 | high-EPA/DHA type antarctic krill oil phospholipid oral liquid and preparation method thereof |
| CN110669581A (en) * | 2019-11-04 | 2020-01-10 | 中粮工科(西安)国际工程有限公司 | Extraction method of microalgae total lipid and microalgae protein meal |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101045258B1 (en) * | 2011-02-11 | 2011-06-30 | 대덕에프알디(주) | Krill oil and method for manufacturing the same |
| CN102041166B (en) * | 2011-01-20 | 2012-05-23 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
| CN102492537A (en) * | 2011-11-14 | 2012-06-13 | 辽宁省大连海洋渔业集团公司 | Method for extracting krill oil from antarctic krill |
| CN102492539A (en) * | 2011-12-22 | 2012-06-13 | 济宁金百特生物机械有限公司 | Method for extracting Euphausia superba oil with continuous countercurrent ultrasonic extracting machine |
| CN102766530A (en) * | 2012-08-08 | 2012-11-07 | 辽宁省大连海洋渔业集团公司 | Method for extracting phospholipid-rich krill oil from Antarctic krill |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100038029A (en) * | 2008-10-02 | 2010-04-12 | 인성실업(주) | Method of producing krill oil using supercritical extraction and krill oil composition with high contents of omega-3 fatty acids |
| CN102071101B (en) * | 2011-01-21 | 2012-10-10 | 山东科芮尔生物制品有限公司 | Method for extracting phospholipid-enriched krill oil from Antarctic krill |
| CN102358865A (en) * | 2011-09-14 | 2012-02-22 | 山东科芮尔生物制品有限公司 | Method of extracting Euphausia superba oil by using supercritical carbon dioxide |
-
2013
- 2013-06-27 CN CN201310262319.7A patent/CN103305335B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102041166B (en) * | 2011-01-20 | 2012-05-23 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
| KR101045258B1 (en) * | 2011-02-11 | 2011-06-30 | 대덕에프알디(주) | Krill oil and method for manufacturing the same |
| CN102492537A (en) * | 2011-11-14 | 2012-06-13 | 辽宁省大连海洋渔业集团公司 | Method for extracting krill oil from antarctic krill |
| CN102492539A (en) * | 2011-12-22 | 2012-06-13 | 济宁金百特生物机械有限公司 | Method for extracting Euphausia superba oil with continuous countercurrent ultrasonic extracting machine |
| CN102766530A (en) * | 2012-08-08 | 2012-11-07 | 辽宁省大连海洋渔业集团公司 | Method for extracting phospholipid-rich krill oil from Antarctic krill |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021194360A1 (en) * | 2020-03-27 | 2021-09-30 | Pharmazen Limited | Extraction method for bio-active fractions |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103305335A (en) | 2013-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103305335B (en) | Low-temperature countercurrent extraction method of euphausia superba sauce | |
| CN102041166B (en) | Method for extracting krill oil with high phosphatide content from Antarctic krills | |
| CN103320217A (en) | Method for extracting krill oil rich in phospholipid from euphausia superba | |
| CN102766530B (en) | Method for extracting phospholipid-rich krill oil from Antarctic krill | |
| CN102358865A (en) | Method of extracting Euphausia superba oil by using supercritical carbon dioxide | |
| Ara et al. | Application of response surface methodology for the optimization of supercritical carbon dioxide extraction and ultrasound-assisted extraction of Capparis spinosa seed oil | |
| CN104479850B (en) | The method of high phospholipid content krill oil is extracted from fresh krill | |
| CN102277230B (en) | Method of optimum rapid solvent extracting treatment for raising extraction amount of microalgae grease | |
| CN103865632B (en) | A kind of delicate fragrance type seed of Flos Moutan mixed oil and preparation method | |
| CN103981021A (en) | Method for refining krill oil from Antarctic krill powder | |
| Miyashita et al. | Bioactive significance of fucoxanthin and its effective extraction | |
| CN106674185B (en) | The method of procyanidine is extracted from Black Box Tracing fruit or pomace | |
| CN104212629A (en) | Method for extracting linseed oil by cold pressing with salt-water wetting | |
| CN105601666B (en) | Method of phosphatide and products thereof is extracted in a kind of fish head from silver carp | |
| WO2015142999A1 (en) | Composition and method to improve blood lipid profiles and reduce low density lipoprotein (ldl) per-oxidation in humans using algae based oils and astaxanthin | |
| CN108840811A (en) | The method that vegetable oil extracts calciferol in mushroom powder | |
| Gong et al. | Process analysis and modeling of a single-step lutein extraction method for wet microalgae | |
| US20170281564A1 (en) | Therapeutic astaxanthin and phospholipid composition and associated method | |
| CN102816638A (en) | Method for extracting phospholipid-rich krill oil from antarctic krills | |
| CN104694244A (en) | Preparation method of krill oil with high esterified astaxanthin and high phospholipid content by double-solvent extraction | |
| Lin et al. | Optimization of enzymatic cell disruption for improving lipid extraction from Schizochytrium sp. through response surface methodology | |
| Tonato et al. | Enhancement of fatty acids in the oil extracted from the fungus Nigrospora sp. by supercritical CO2 with ethanol as a cosolvent | |
| CN109097190B (en) | Method for enriching phospholipid from krill oil | |
| CN103864841A (en) | Method for extracting and refining phospholipid from egg of aquatic animal | |
| US20210214711A1 (en) | Method for obtaining fat-soluble and water-soluble compounds from microalgae by modulating the polarity of vegetable or animal oils |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Shaofang Inventor after: Yu Zhipeng Inventor after: Yang Qingli Inventor after: Cao Rongjun Inventor after: Ren Xianjun Inventor before: Liu Shaofang Inventor before: Yu Zhipeng Inventor before: Xue Changhu Inventor before: Yang Qingli Inventor before: Cao Rongjun Inventor before: Wu Xin Inventor before: Ren Xianjun |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LIU SHAOFANG YU ZHIPENG XUE CHANGHU YANG QINGLI CAO RONGJUN WU XIN REN XIANJUN TO: LIU SHAOFANG YU ZHIPENG YANG QINGLI CAO RONGJUN REN XIANJUN |
|
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Shaofang Inventor after: Yu Zhipeng Inventor after: Yang Qingli Inventor after: Cao Rongjun Inventor after: Wu Xin Inventor after: Ren Xianjun Inventor before: Liu Shaofang Inventor before: Yu Zhipeng Inventor before: Xue Changhu Inventor before: Yang Qingli Inventor before: Cao Rongjun Inventor before: Wu Xin Inventor before: Ren Xianjun |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LIU SHAOFANG YU ZHIPENG XUE CHANGHU YANG QINGLI CAO RONGJUN WU XIN REN XIANJUN TO: LIU SHAOFANG YU ZHIPENG YANG QINGLI CAO RONGJUN WU XIN REN XIANJUN |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150429 Termination date: 20190627 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |