CN103293119A - Method for rapidly detecting contents of amaranthus red and brilliant blue in foods - Google Patents
Method for rapidly detecting contents of amaranthus red and brilliant blue in foods Download PDFInfo
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- CN103293119A CN103293119A CN2013101955087A CN201310195508A CN103293119A CN 103293119 A CN103293119 A CN 103293119A CN 2013101955087 A CN2013101955087 A CN 2013101955087A CN 201310195508 A CN201310195508 A CN 201310195508A CN 103293119 A CN103293119 A CN 103293119A
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Abstract
The invention provides a method for rapidly detecting the contents of amaranthus red and brilliant blue in foods. The method comprises the following steps of: respectively taking an amaranthus red standard solution, a brilliant blue pigment standard solution and 0.3mL of ion liquid into a colorimetric tube; diluting and making up to volume being 10mL; determining the absorbance of the amaranthus red and brilliant blue pigment standard solutions on a 522nm place and a 630nm place by utilizing an ultraviolet and visible spectrophotometer, and making working curves to obtain equations of linear regression; filtering a liquid food sample or solid food sample dissolving solution and extracting by an ion liquid double-water-phase system formed by 1-butyl-3-methylimidazole bromide and K2PHO4; diluting an ion liquid phase to a constant volume and determining the absorbance of the system; respectively calculating to obtain the contents of the amaranthus red and the brilliant blue in the foods according to the equations of linear regression. The method disclosed by the invention is easy to operate and does not need to use a plurality of volatile solvents; the separation time is short and an instrument is common and easy to obtain; the method disclosed by the invention can be used for performing rapid, convenient, environment-friendly and economical qualitative and quantitative determination for quality inspection departments and manufacturing enterprises.
Description
Technical field
The present invention relates to the detection method of pigment content in a kind of food, particularly the amaranth in a kind of fast detecting food and the method for light blue content belong to food security adjuvant detection technique field.
Background technology
Amaranth and light blue are to use two kinds of higher synthetic food colors of frequency, are widely used in the production of varieties of food items.Adding in the time of these two kinds of pigments to make food present purple.But have the researcher to find that there is certain insecurity in such food coloring or produces illeffects human body in recent years, though the harmfulness of edible synthesized coloring matter final conclusion not still, they are without any nutritive value, to health also without any help.At this kind situation, various countries hygiene department is all to use the behavior of edible synthesized coloring matter in the formal Specification food production of laws and regulations.China has not only formulated " food additives use hygienic standard ", has stipulated that clearly edible synthesized coloring matter allows kind and the use amount of using, and has also formulated the national standard method of food coloring analyzing and testing.Therefore, the mensuration for synthetic coloring matter in the food is very important.
The subject matter that influences at present the pigment adjuvant mensuration in the beverage is the interference that has multiple pigment and other related substanceses in the sample.Therefore the preenrichment technology usually is used for the compartment analysis of synthetic food color, as liquid-liquid extraction (LLE) and Solid-Phase Extraction (SPE) etc.LLE often needs to use poisonous organic solvent, and liquid-liquid extraction is more time-consuming loaded down with trivial details.The SPE technology is a kind of purification technique preferably, but desorb need use and have volatile organic solvent, and consuming time relatively longer.And double-aqueous phase system (ATPS) is to be formed by the specific salt of certain class that high concentration is mixed or added in a kind of superpolymer aqueous solution to two kinds of different superpolymer aqueous solution of structure mutually, and this technology can reduce organic solvent to the harm of environment.Being extracted the distribution of material in two-phase can become the composition of phase temperature and salt to come system is optimized by change pH value of solution, system.By double-aqueous phase system, sample can purify simultaneously, extraction and concentrated.Yet the superpolymer that generates in traditional superpolymer aqueous two phase extraction technique is mostly mutually to be the opaque solution of high viscosity, brings difficulty for the follow-up mensuration that is extracted material.
Summary of the invention
Technical matters to be solved by this invention is at the deficiencies in the prior art, provide a kind of fast, accurately, the amaranth in the qualitative and quantitative fast detecting food and the method for light blue content intuitively.
Technical matters to be solved by this invention is to realize by following technical scheme.The present invention is amaranth in a kind of fast detecting food and the method for light blue content, and be characterized in: its step is as follows:
(1) making of working curve: getting amaranth and the 0.015～1.50mL concentration that 0.04～4.0mL concentration is 100 μ g/mL respectively is 100 μ g/mL light blue pigment standard solution, put into color comparison tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt more separately respectively, to 10mL, measure the absorbance of pigment standard solution with ultraviolet-visible pectrophotometer: the detection wavelength of amaranth and light blue is respectively 522nm and 630nm with distilled water diluting; Make working curve, obtain equation of linear regression;
(2) sample preparation: food samples is fluid sample or solid sample, adopts following method to handle respectively:
Fluid sample: get sample that the 2.0mL suction filtration crosses in the 10mL centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be settled to 4mL, add 3.6g K
2HPO
4, vibration is to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
Solid sample: take by weighing the 2.0-5.0g solid food, use the 10-20mL dissolved in distilled water, get this solution of 3mL to 10mL tool plug centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be diluted to 4mL, add 3.6g K
2HPO
4, vibration is to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
(3) according to the equation of linear regression of step (1), calculate the content of amaranth and light blue in fluid sample or the solid sample respectively.
Compared with prior art, the advantage of the inventive method is mainly reflected in:
1, the inventive method uses ionic liquid/salt aqueous two-phase extraction as the pre-treating method of example enrichment, makes that the interference of other non-pigmented adjuvants reduces greatly in the food, pigment light blue and amaranth in can more accurate detection food.
2, because material has selectivity for the absorption of light, so different food coloring is had different different absorption spectrums.Utilize this principle, use ultra-violet and visible spectrophotometer light blue and amaranth pigment are carried out absorption spectrum scanning, and with the contrast of standard spectrogram, just can be intuitively, qualitative fast.Because the maximum absorption wavelength of pigment light blue and amaranth differs 108nm, to measure noiselessly substantially, sensitivity and accuracy are all higher.
3, method of operating of the present invention is simple, need not use a large amount of volatile solvents, disengaging time is short, instrument is common to be easy to get, and calculates easyly, and the result is obvious, used instrument low price, detect with low costly, be fit to the production line and use, can provide quick, easy, environmental protection, economic qualitative and quantitative measurement for quality testing department and manufacturing enterprise.
Embodiment
Below by example the present invention is further described, but protection scope of the present invention is not limited to this.
Embodiment 1, the amaranth in a kind of fast detecting food and the method for light blue content, and its step is as follows:
(1) making of working curve: getting amaranth and the 0.015～1.50mL concentration that 0.04～4.0mL concentration is 100 μ g/mL respectively is 100 μ g/mL light blue pigment standard solution, put into color comparison tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt more separately respectively, to 10mL, measure the absorbance of pigment standard solution with ultraviolet-visible pectrophotometer: the detection wavelength of amaranth and light blue is respectively 522nm and 630nm with distilled water diluting; Make working curve, obtain equation of linear regression;
(2) sample preparation: food samples is fluid sample or solid sample, adopts following method to handle respectively:
Fluid sample: get sample that the 2.0mL suction filtration crosses in the 10mL centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be settled to 4mL, add 3.6g K
2HPO
4, vibration is to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
Solid sample: take by weighing the 2.000-5.000g solid food, use the 10-20mL dissolved in distilled water, get this solution of 3mL to 10mL tool plug centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be diluted to 4mL, add 3.6g K
2HPO
4, vibration is to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
(3) according to the equation of linear regression of step (1), calculate the content of amaranth and light blue in fluid sample or the solid sample respectively.
Embodiment 2, the amaranth in the fast detecting grape flavor soda and the method experiment of light blue content, and concrete steps are as follows:
1, working curve is made: method is with method in example 1 step 1.
Table 1: the equation of linear regression of pigment and correlated results
| Pigment | Working curve | The range of linearity (μ g/mL) | Molar absorptivity ε (L/molcm) | Detection limit (μ g/mL) | RSD(%) |
| Amaranth | Y=0.0231x+0.0031 | 0.40-40.0 | 1.40×10 5 | 0.034 | 0.026 |
| Light blue | Y=0.0952x-0.0034 | 0.15-15.0 | 7.55×10 5 | 0.011 | 0.035 |
2, sample preparation
Get 3.00mL board grape flavor beverage in 10mL tool plug centrifuge tube, ultrasonic heating 10min removes CO
2, add 0.3mL1-butyl-3-methylimidazole bromine salt ion liquid, to the 4mL scale, add 3.5g K with distilled water diluting
2HPO
4Vibration is to dissolving fully, the centrifugal 2-3min of 3000r/min, form aqueous two-phase,, with syringe upper strata ionic liquid phase shift is taken in the 10mL color comparison tube, be diluted to scale, under 522nm and 630nm wavelength, measure absorbance respectively, according to the working curve that table 1 obtains, calculate that the content of light blue and amaranth is respectively 0.38 μ g/mL and 15.27 μ g/mL in certain board grape flavor beverage.
Embodiment 3, the method for light blue content experiment in the fast detecting blue berry flavor sports drink, and concrete steps are as follows:
1, working curve is made: get 0.015,0.05,0.10,0.20,0.40,0.60,0.80,1.00,1.50ml 100 μ g/mL light blue standard solution, put into the 10mL color comparison tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt more separately respectively, be diluted to scale with the distillation art, measure absorbance at 630nm wavelength place; Make working curve, obtain equation of linear regression, molar absorptivity, detection limit and relative standard deviation and see Table 1.
2, sample preparation
With the ultrasonic 5min of beverage, fully remove carbon dioxide, get the 3.00mL beverage in 10mL tool plug centrifuge tube, add 0.3mL1-butyl-3-methylimidazole bromine salt, to the 4mL scale, add 3.5g K with distilled water diluting
2HPO
4Vibration is to dissolving fully, the centrifugal 2-3min of 3000r/min, form aqueous two-phase, with syringe upper strata ionic liquid phase shift is taken in the 10mL color comparison tube, distilled water diluting is measured absorbance to scale under the 630nm wavelength, according to the equation of linear regression that step 1 obtains, the content that calculates light blue in the blue berry flavor sports drink is 0.32 μ g/mL.
Embodiment 4, the method for light blue content experiment in the fast detecting peppermint candy, and concrete steps are as follows:
1, working curve is made: method is with method in example 1 step 1.
2, sample preparation
Get the 5.000g peppermint candy, use the 10ml dissolved in distilled water, extract wherein pigment at twice, merge extract, pipette 3mL in 10mL tool plug centrifuge tube, add 0.3mL1-butyl-3-methylimidazole bromine salt, to scale, add 3.5g K with distilled water diluting
2HPO
4Vibration is to dissolving fully, the centrifugal 2-3min of 3000r/min, form aqueous two-phase, with syringe upper strata ionic liquid phase shift is taken in the 10mL color comparison tube, distilled water diluting is measured absorbance to scale under the 630nm wavelength, according to the working curve that obtains in the step 1, calculate that the content of light blue is respectively 0.05 μ g/g in the peppermint candy.
Claims (1)
1. the amaranth in the fast detecting food and the method for light blue content, it is characterized in that: its step is as follows:
(1) making of working curve: getting amaranth and the 0.015～1.50mL concentration that 0.04～4.0mL concentration is 100 μ g/mL respectively is 100 μ g/mL light blue pigment standard solution, put into color comparison tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt more separately respectively, to 10mL, measure the absorbance of pigment standard solution with ultraviolet-visible pectrophotometer: the detection wavelength of amaranth and light blue is respectively 522nm and 630nm with distilled water diluting; Make working curve, obtain equation of linear regression;
(2) sample preparation: food samples is fluid sample or solid sample, adopts following method to handle respectively:
Fluid sample: get sample that the 2.0mL suction filtration crosses in the 10mL centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be settled to 4mL, add 3.6g K
2HPO
4, vibration is to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
Solid sample: take by weighing the 2.0-5.0g solid food, use the 10-20mL dissolved in distilled water, get this solution of 3mL to 10mL tool plug centrifuge tube, add 0.3mL ionic liquid 1-butyl-3-methylimidazole bromine salt, be diluted to 4mL, add 3.6g K
2HPO
4, vibration shakes up to dissolving fully, and the centrifugal 2-3min of 3000r/min forms aqueous two-phase, gets to color comparison tube and is diluted to 10mL, the mensuration absorbance;
(3) according to the equation of linear regression of step (1), calculate the content of amaranth and light blue in fluid sample or the solid sample respectively.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198414A (en) * | 2014-09-05 | 2014-12-10 | 北京智云达科技有限公司 | Method for detecting artificially synthesized edible pigments |
| CN107091897A (en) * | 2017-06-02 | 2017-08-25 | 杨大伟 | A kind of method of tonyred in ionic liquid aqueous two-phase extraction HPLC methods detection capsicum |
| CN108287092A (en) * | 2017-12-29 | 2018-07-17 | 东莞理工学院 | For detecting synthetic dyestuff standard sample and preparation method thereof in hard candy |
| CN110915765A (en) * | 2019-12-19 | 2020-03-27 | 四川农业大学 | Drosophila feed intake detection method |
| CN112285041A (en) * | 2020-09-30 | 2021-01-29 | 江苏大学 | Rapid detection method for ionic liquid content |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102645415A (en) * | 2012-04-13 | 2012-08-22 | 昆明理工大学 | Method for quickly detecting content of amaranth and sunset yellow in foods |
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- 2013-05-24 CN CN2013101955087A patent/CN103293119A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102645415A (en) * | 2012-04-13 | 2012-08-22 | 昆明理工大学 | Method for quickly detecting content of amaranth and sunset yellow in foods |
Non-Patent Citations (2)
| Title |
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| 刘培元等: "离子液体/盐双水相萃取技术的研究进展", 《化学工程与装备》 * |
| 邓凡政等: "离子液体双水相萃取分离苋菜红的研究", 《分析试验室》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198414A (en) * | 2014-09-05 | 2014-12-10 | 北京智云达科技有限公司 | Method for detecting artificially synthesized edible pigments |
| CN107091897A (en) * | 2017-06-02 | 2017-08-25 | 杨大伟 | A kind of method of tonyred in ionic liquid aqueous two-phase extraction HPLC methods detection capsicum |
| CN107091897B (en) * | 2017-06-02 | 2019-09-13 | 上海博焱检测技术服务有限公司 | A kind of method that ionic liquid aqueous two-phase extraction-HPLC method detects tonyred in capsicum |
| CN108287092A (en) * | 2017-12-29 | 2018-07-17 | 东莞理工学院 | For detecting synthetic dyestuff standard sample and preparation method thereof in hard candy |
| CN110915765A (en) * | 2019-12-19 | 2020-03-27 | 四川农业大学 | Drosophila feed intake detection method |
| CN110915765B (en) * | 2019-12-19 | 2021-06-25 | 四川农业大学 | Drosophila feed intake detection method |
| CN112285041A (en) * | 2020-09-30 | 2021-01-29 | 江苏大学 | Rapid detection method for ionic liquid content |
| CN112285041B (en) * | 2020-09-30 | 2023-04-11 | 江苏大学 | Rapid detection method for ionic liquid content |
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Application publication date: 20130911 |