CN103284728B - Application of perylene derivative - Google Patents
Application of perylene derivative Download PDFInfo
- Publication number
- CN103284728B CN103284728B CN201310161017.0A CN201310161017A CN103284728B CN 103284728 B CN103284728 B CN 103284728B CN 201310161017 A CN201310161017 A CN 201310161017A CN 103284728 B CN103284728 B CN 103284728B
- Authority
- CN
- China
- Prior art keywords
- laten fingerprints
- transfer film
- perylene
- developing agent
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides an application of a perylene derivative, belongs to the field of detection techniques, and solves the technical problems that the conventional developing method of latent fingerprints is cumbersome and time-consuming, pollutes the environment, needs complex instruments for assistance, has more requirements on the property and surface state of a to-be-developed object, and cannot clearly develop a knotty object. The perylene derivative provided by the invention can serve as a developing agent and is prepared into a developing solution used for the development of the latent fingerprints. As the developing agent, the perylene derivative provided by the invention can quickly and simply develop the latent fingerprints, is economical and practical, has high sensitivity, does not need the complex instruments for assistance, is harmless to the environment and operating personnel and has a wide application range, and the developed veins of the latent fingerprints can be preserved for a long time.
Description
Technical field
The present invention relates to a kind of application of perylene derivant, this perylene derivant can manifest laten fingerprints as developing agent, belongs to detection technique field.
Background technology
Fingerprint is that finger tips refers to abdomen upper epidermis fold and the concave convex texture formed or its impression stayed, and has people variant, and the feature of constant and tactile thing trace, was once called " first of evidence " by judicial circuit throughout one's life.Fingerprint can be divided three classes: obviously stricture of vagina (hands is stained with the article transfer printings such as paint, blood, ink and is formed), molding stricture of vagina (finger contact is stamped on the soft material such as wax candle, clay and forms) and visual sightless laten fingerprints (the secreted thing of finger and the invisible contaminant transfer printing of carrying thereof form).
Need certain process for show could observe its fingerprint lines for visual sightless laten fingerprints, in prior art, the process for show of laten fingerprints mainly contains optical method, physics appearance method, chemical appearance method and physical chemistry appearance method.Optics appearance method generally needs complicated instrument to assist, and be unsuitable for field assay, as luminescence method needs chemiluminescence instrument, infrared spectrometry and mass spectrography need spectrogrph, and bounce technique needs laser lamp etc.; Physics appearance method requires higher for the development character of object and apparent condition, and as powder method is difficult to manifest at moist surface, water logging object and rough surface, vacuum metal deposition method is difficult to manifest at porous surface; Chemistry appearance method, as ninhydrin method and DFO method, due to 1,2,3-indantrione monohydrate and DFO at room temperature sluggish, and can and fingerprint in chemical constituent react, cause developing time to be grown, affect the further analysis of fingerprint; Physical chemistry appearance method, as many precipitated metals method, complex steps, is unfavorable for operation.In addition, be mostly difficult to realize the clear of laten fingerprints on the difficult object such as fabric and skin in said method manifest.
Perylene derivant is owing to having fluorescent characteristic, in recent years, be widely used in dyestuff, battery, the fields such as Small-molecule probe, fluorescent small molecule probe detecting enzymatic activity as Chinese patent is a kind of and preparation method thereof and application (publication number 102796515A, publication date 2012.11.28), prepare a kind of perylene derivant, this derivant has obvious green fluorescence in aqueous, can realize enzymatic activity and quick, the sensitive detection of inhibitor activity as Small-molecule probe.But in prior art, the not report applied as developing agent of this perylene derivant.
Summary of the invention
The process for show that the object of the invention is to solve existing laten fingerprints is loaded down with trivial details, length consuming time, contaminated environment, complicated instrument is needed to assist, to character, the apparent condition requirement technical problem that is higher and that cannot clearly manifest difficult object of development object, provide a kind of application of perylene derivant (PTTAC).
The invention provides the application of a kind of perylene derivant as developing agent, the structural formula of described perylene derivant is:
In formula, n is the integer of 2 ~ 8.
Preferably, described perylene derivant, as the application of developing agent, comprises the following steps:
(1) perylene derivant being dissolved in pH value is be mixed with the perylene derivant developer solution that perylene derivatives concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) object being loaded with laten fingerprints is immersed in 5s ~ 4h in perylene derivant developer solution, obtains the object manifesting laten fingerprints;
(3) under uviol lamp, irradiate the object manifesting laten fingerprints and namely obtain fingerprint lines.
Preferably, also comprise, the object manifesting laten fingerprints described in step (2) is rinsed, it is further preferred that by dry for the object manifesting laten fingerprints after rinsing.
Preferably, described perylene derivant, as the application of developing agent, comprises the following steps:
(1) perylene derivant being dissolved in pH value is be mixed with the perylene derivant developer solution that perylene derivatives concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) transfer film is placed in the object surface being loaded with laten fingerprints, laten fingerprints extracts on transfer film by pressing transfer film;
(3) transfer film being loaded with laten fingerprints is immersed in 5s ~ 10s in perylene derivant developer solution, obtains the transfer film manifesting laten fingerprints;
(4) under uviol lamp, irradiate the transfer film manifesting laten fingerprints and namely obtain fingerprint lines.
Preferably, described transfer film is celluloid transfer film or Kynoar (PVDF) transfer film.
Preferably, also comprise, the transfer film manifesting laten fingerprints described in step (3) is rinsed, it is further preferred that by dry for the transfer film manifesting laten fingerprints after rinsing.
Preferably, described buffer is Tris-HCl buffer or phosphate (PBS) buffer, and concentration is 5mM ~ 100mM.
Preferably, be the NaCl of 0 ~ 200mM and concentration containing concentration in described buffer be the MgCl of 0 ~ 15mM
2.
The invention has the beneficial effects as follows:
(1) perylene derivant of the present invention manifests laten fingerprints as developing agent, by the PTTAC molecule containing positive charge and fingerprint residues thing generation Electrostatic Absorption and hydrophobic interaction, excimer is become in fingerprint residues thing set of surfaces combinate form, under the irradiation of exciting light, send fluorescence thus show fingerprint stricture of vagina road clearly, when using transfer film to manifest laten fingerprints, utilize the contact of transfer film and laten fingerprints, the residue of laten fingerprints is transferred on transfer film, obtain with non-transfer before fingerprint lines be mutually the fingerprint stricture of vagina road of mirror image, and then manifesting of laten fingerprints is carried out to transfer film,
(2) perylene derivant of the present invention manifests laten fingerprints simple and quick (using transfer film to manifest laten fingerprints to realize in 5s ~ 10s) as developing agent, the developer solution of preparation can reuse, economical and practical, operating process does not need complicated instrument and equipment, can be used for Site Detection, adopt fluorescence as signal, background is low, highly sensitive, and do not pollute object surface, to environment and operator's nonhazardous;
(3) perylene derivant of the present invention manifests laten fingerprints as developing agent and can be applicable to impermeability object and partial permeability object, dry object surface and water-disposed object surface all can use, and can manifest outmoded aging laten fingerprints, when using transfer film to manifest laten fingerprints, the laten fingerprints on all smooth surface objects and the laten fingerprints on a part of rough surface object can be manifested, as the laten fingerprints on the difficult object such as permeable fiber fabric, skin, dust of larger aperture can be manifested;
(4) perylene derivant of the present invention manifests laten fingerprints as developing agent, and the fingerprint lines manifested is obviously clear, without significant change in four months, can preserve for a long time.
Accompanying drawing explanation
Fig. 1 is the developing latent finger prints of the embodiment of the present invention 1 on glass fingerprint lines figure out;
Fig. 2 is the developing latent finger prints of the embodiment of the present invention 2 on PVDF transfer film fingerprint lines figure out;
Fig. 3 is the developing latent finger prints of the embodiment of the present invention 3 on aluminium foil fingerprint lines figure out;
Fig. 4 is the embodiment of the present invention 4 aging developing latent finger prints fingerprint lines figure out of month on glass;
Fig. 5 is the developing latent finger prints of the embodiment of the present invention 5 on porcelain fingerprint lines figure out;
Fig. 6 is the embodiment of the present invention 6 developing latent finger prints on stainless steel fingerprint lines figure out;
Fig. 7 is the developing latent finger prints of the embodiment of the present invention 7 in technicolo insulating tape adhesive faces fingerprint lines figure out;
Fig. 8 is the developing latent finger prints of the embodiment of the present invention 8 on celluloid transfer film fingerprint lines figure out;
Fig. 9 is the developing latent finger prints of the embodiment of the present invention 9 on plastics fingerprint lines figure out;
Figure 10 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 10 transfer film extracts on computer screen;
Figure 11 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 11 transfer film extracts on glass;
Figure 12 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 12 transfer film extracts on coin;
Figure 13 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 13 transfer film extracts on A4 paper;
Figure 14 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 14 transfer film extracts on mouse pad;
Figure 15 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 15 transfer film extracts on skin.
Detailed description of the invention
Perylene derivant is as the application of developing agent, and the structural formula of described perylene derivant is:
In formula, n is the integer of 2 ~ 8, and ⊕ represents positive charge.
Perylene derivant, as the application of developing agent, comprises the following steps:
(1) PTTAC being dissolved in pH value is be mixed with the PTTAC developer solution that PTTAC concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) immersed in PTTAC developer solution by the object being loaded with laten fingerprints, the color according to fingerprint on object changes, and takes out object, obtain the object manifesting laten fingerprints after 5s ~ 4h;
(3) object manifesting laten fingerprints is irradiated under uviol lamp namely obtain fingerprint lines, if think, fingerprint lines is more obvious, the object manifesting laten fingerprints can be rinsed, after removing unnecessary developer solution, use ultra violet lamp again, if think, fingerprint lines is more clear, can by after rinsing manifest the object drying of laten fingerprints after, then use ultra violet lamp.
Perylene derivant, as the application of developing agent, comprises the following steps:
(1) PTTAC being dissolved in pH value is be mixed with the PTTAC developer solution that PTTAC concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) transfer film is placed in the object surface being loaded with laten fingerprints, laten fingerprints extracts on transfer film by pressing transfer film;
(3) transfer film being loaded with laten fingerprints is immersed 5s ~ 10s in PTTAC developer solution, obtain the transfer film manifesting laten fingerprints;
(4) transfer film manifesting laten fingerprints is irradiated under uviol lamp namely obtain fingerprint lines, if think, fingerprint lines is more obvious, the transfer film manifesting laten fingerprints can be rinsed, after removing unnecessary developer solution, use ultra violet lamp again, if think, fingerprint lines is more clear, can by after rinsing manifest the drying of laten fingerprints after, then use ultra violet lamp; The fingerprint lines that the fingerprint lines that step (4) obtains and object are loaded with is mutually mirror image, is those skilled in the art's known technologies by the fingerprint lines that the fingerprint lines on transfer film converts on object.
In present embodiment, the concentration of PTTAC is preferably 100 μMs ~ 500 μMs.
In present embodiment, the concentration of buffer is preferably 5mM ~ 100mM, pH value is preferably 7.0 ~ 9.0, and usually use Tris-HCl buffer or PBS buffer, Tris-HCl buffer is the mixed liquor of Tris (Tris) solution and hydrochloric acid (HCl); PBS buffer solution is sodium dihydrogen phosphate (NaH
2pO
4) and sodium hydrogen phosphate (Na
2hPO
412H
2o).
In present embodiment, preferably containing NaCl and MgCl in buffer
2in one or both, in buffer, the concentration of NaCl is 0 ~ 200mM, MgCl
2concentration be 0 ~ 15mM.
In present embodiment, transfer film is known to the skilled person technology, for for the transfer of protein and nucleic acid and the transfer film of testing process, is preferably celluloid transfer film or PVDF transfer film.
In present embodiment, when rinsing the object manifesting laten fingerprints or the transfer film manifesting laten fingerprints, particular/special requirement is not had to flushing liquor, generally adopts tap water, pure water or distilled water.
In present embodiment, dry only otherwise fingerprint texture affect on object, adopt any method can, drying can be adopted in laboratory or blot with filter paper, more usually naturally dry or dry up, drying up the drying baker that can use within hair-dryer or 80 DEG C.
In present embodiment, the preferred excitation wavelength of uviol lamp is at the uviol lamp of 365nm.
In present embodiment, perylene derivant (PTTAC) is prior art, see a kind of fluorescent small molecule probe detecting enzymatic activity of Chinese patent and preparation method thereof and application (publication number 102796515A, publication date 2012.11.28), present embodiment provides a kind of preparation method of perylene derivant, but the present invention is not limited thereto, detailed process is:
(1) be dissolved in aqueous slkali by perylene dicarboxylic anhydride, filter, filtrate adjust ph to 8 ~ 9, add four n-octyl ammonium bromide and two bromoalkanes, at 100 ~ 120 DEG C of back flow reaction 1 ~ 3h, through extraction, distillation and purification, obtain compound, described perylene dicarboxylic anhydride, the mol ratio of four n-octyl ammonium bromide and two bromoalkanes is 1:0.1:10, and two described bromoalkanes are glycol dibromide, 1,3-dibromopropane, Isosorbide-5-Nitrae-dibromobutane, pentamethylene bromide, 1,6-dibromo-hexane, 1,7-dibromo-heptane or 1,8-bis-bromooctane, described compound is 3,4,9,10-tetra--(2-bromoethyl-ester base) perylene, 3,4,9,10-tetra--(3-bromopropyl-ester base) perylene, 3,4,9,10-tetra--(4-brombutyl-ester base) perylene, 3,4,9,10-tetra--(5-bromine amyl group-ester base) perylene, 3,4,9,10-tetra--(6-bromine hexyl-ester base) perylene, 3,4,9,10-, tetra--(7-bromine heptyl-ester base) perylene or 3,4,9,10-tetra--(8-bromine octyl group-ester base) perylene,
(2) compound obtained in step (1) is dissolved in the mixed liquor of oxolane and water, add trimethylamine, 60 ~ 70 DEG C of backflow 48 ~ 96h, after distillation, extraction and drying, obtain PTTAC, described compound and the mol ratio of trimethylamine are 1:20.
In present embodiment, mentioned M all represents mol/L, and object represents any object that can carry fingerprint, is all art's common art-recognized meanings.
Embodiment 1
Composition graphs 1 illustrates embodiment 1
Perylene derivant manifests the application of laten fingerprints as developing agent:
(1) preparation of PTTAC (n=2) developer solution of 1mM: the PTTAC (n=2) taking 256mg, is dissolved in the Tris-HCl buffer of the 100mM of 200mL, fully dissolve mixing and be placed on lucifuge place, buffer contains the MgCl of 15mM
2, the pH value of buffer is 8.5;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare glass immersion step (1) being loaded with laten fingerprints, take out after 60min, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the glass of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 1 is the developing latent finger prints of the embodiment of the present invention 1 on glass fingerprint lines figure out, and as can be seen from Figure 1, it is obviously clear that PTTAC (n=2) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 2
Composition graphs 2 illustrates embodiment 2
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=6) developer solution of (1) 10 μM: the PTTAC (n=6) taking 3.01mg, be dissolved in the PBS buffer of the 5mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the NaCl containing 200mM in buffer, and the pH value of buffer is 8.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare PVDF transfer film immersion step (1) being loaded with laten fingerprints, take out after 30s, wash away unnecessary PTTAC developer solution with clear water, dry up;
(3) be loaded with the PVDF transfer film of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 2 is the developing latent finger prints of the embodiment of the present invention 2 on PVDF transfer film fingerprint lines figure out, and as can be seen from Figure 2, it is obviously clear that PTTAC (n=6) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 3
Composition graphs 3 illustrates embodiment 3
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=5) developer solution of (1) 500 μM: the PTTAC (n=5) taking 145mg, be dissolved in the Tris-HCl buffer of the 20mM of 200mL, abundant dissolving mixing is placed on lucifuge place, NaCl containing 100mM in buffer, the pH value of buffer is 7.5;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare aluminium foil immersion step (1) being loaded with laten fingerprints, take out after 10min, wash away unnecessary PTTAC developer solution with clear water, dry up;
(3) be loaded with the aluminium foil of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 3 is the developing latent finger prints of the embodiment of the present invention 3 on aluminium foil fingerprint lines figure out, and as can be seen from Figure 3, it is obviously clear that PTTAC (n=5) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 4
Composition graphs 4 illustrates embodiment 4
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=5) developer solution of (1) 200 μM: the PTTAC (n=5) taking 57.9mg, be dissolved in the Tris-HCl buffer of the 60mM of 200mL, abundant dissolving mixing is placed on lucifuge place, NaCl containing 20mM in buffer, the pH value of buffer is 7.0;
(2) laten fingerprints is manifested: the sheet glass of laten fingerprints of month immerses in step (1) the PTTAC developer solution prepared by being loaded with aging, takes out, wash away unnecessary PTTAC developer solution, dry up with clear water after 2h;
(3) be loaded with the sheet glass of laten fingerprints of month aging with the ultra violet lamp of 365nm, obtained fingerprint lines clearly.
Fig. 4 is the embodiment of the present invention 4 aging developing latent finger prints fingerprint lines figure out of month on glass, as can be seen from Figure 4, it is obviously clear that PTTAC (n=5) manifests laten fingerprints fingerprint lines as developing agent, and can be used for manifesting of outmoded aging laten fingerprints.
Embodiment 5
Composition graphs 5 illustrates embodiment 5
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=7) developer solution of (1) 500 μM: the PTTAC (n=7) taking 156mg, be dissolved in the PBS buffer of the 20mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 15mM containing 50mM in buffer
2, the pH value of buffer is 9.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare porcelain immersion step (1) being loaded with laten fingerprints, take out after 30min, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the porcelain of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 5 is the developing latent finger prints of the embodiment of the present invention 5 on porcelain fingerprint lines figure out, and as can be seen from Figure 5, it is obviously clear that PTTAC (n=7) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 6
Composition graphs 6 illustrates embodiment 6
Perylene derivant manifests the application of laten fingerprints as developing agent:
(1) preparation of PTTAC (n=4) developer solution of 10mM: the PTTAC (n=4) taking 2.79g, is dissolved in the Tris-HCl buffer of the 100mM of 200mL, fully dissolve mixing and be placed on lucifuge place, the pH value of buffer is 7.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare rustless steel immersion step (1) being loaded with laten fingerprints, take out after 20min, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the rustless steel object of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 6 is the embodiment of the present invention 6 developing latent finger prints on stainless steel fingerprint lines figure out, and as can be seen from Figure 6, it is obviously clear that PTTAC (n=4) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 7
Composition graphs 7 illustrates embodiment 7
Perylene derivant manifests the application of laten fingerprints as developing agent:
(1) preparation of PTTAC (n=3) developer solution of 2mM: the PTTAC (n=3) taking 27.8mg, is dissolved in the PBS buffer of the 100mM of 200mL, fully dissolves mixing and is placed on lucifuge place, the MgCl containing 15mM in buffer
2, the pH value of buffer is 8.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution that bottle green electrical insulating tape immersion step (1) adhesive faces being loaded with laten fingerprints is prepared, take out after 4h, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the bottle green electrical insulating tape of laten fingerprints by the ultra violet lamp adhesive faces of 365nm, obtain fingerprint lines clearly.
Fig. 7 is the developing latent finger prints of the embodiment of the present invention 7 in technicolo insulating tape adhesive faces fingerprint lines figure out, and as can be seen from Figure 7, it is obviously clear that PTTAC (n=3) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 8
Composition graphs 8 illustrates embodiment 8
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=4) developer solution of (1) 50 μM: the PTTAC (n=4) taking 13.9mg, be dissolved in the Tris-HCl buffer of the 40mM of 200mL, abundant dissolving mixing is placed on lucifuge place, NaCl containing 50mM in buffer, the pH value of buffer is 8.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare nitrocellulose filter immersion step (1) being loaded with laten fingerprints, take out after 15s, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the nitrocellulose filter of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 8 is the developing latent finger prints of the embodiment of the present invention 8 on nitrocellulose filter fingerprint lines figure out, and as can be seen from Figure 8, it is obviously clear that PTTAC (n=4) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 9
Composition graphs 9 illustrates embodiment 9
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=3) developer solution of (1) 300 μM: the PTTAC (n=3) taking 80.2mg, be dissolved in the Tris-HCl buffer of the 80mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 15mM containing 20mM in buffer
2, the pH value of buffer is 7.0;
(2) laten fingerprints is manifested: in the PTTAC developer solution prepare plastics immersion step (1) being loaded with laten fingerprints, take out after 2h, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(3) be loaded with the plastics of laten fingerprints with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Fig. 9 is the developing latent finger prints of the embodiment of the present invention 9 on plastics fingerprint lines figure out, and as can be seen from Figure 9, it is obviously clear that PTTAC (n=3) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 10
In conjunction with Figure 10, embodiment 10 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=4) developer solution of (1) 1 μM: the PTTAC (n=4) taking 2.78mg, be dissolved in the PBS buffer of the 5mM of 2L, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 5mM containing 100mM in buffer
2, the pH value of buffer is 6.5;
(2) extraction of laten fingerprints: PVDF transfer film is close to the laten fingerprints place on computer screen, firmly presses transfer film, makes fingerprint fully be transferred on pvdf membrane;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare PVDF transfer film immersion step (1) being extracted laten fingerprints on computer screen, take out after 1min, wash away unnecessary PTTAC developer solution with clear water, dry up;
(4) be extracted the PVDF transfer film of laten fingerprints on computer screen with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 10 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 10 transfer film extracts on computer screen, and as can be seen from Figure 10, it is obviously clear that PTTAC (n=4) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 11
In conjunction with Figure 11, embodiment 11 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=3) developer solution of (1) 50 μM: the PTTAC (n=3) taking 13.4mg, be dissolved in the PBS buffer of the 10mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the NaCl containing 100mM in buffer, and the pH value of buffer is 7.5;
(2) extraction of laten fingerprints: PVDF transfer film is close to the laten fingerprints place on glass, firmly presses transfer film, makes fingerprint fully be transferred on pvdf membrane;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare pvdf membrane immersion step (1) being extracted laten fingerprints on glass, take out after 10s, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(4) be extracted the pvdf membrane of laten fingerprints on glass with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 11 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 11 transfer film extracts on glass, and as can be seen from Figure 11, it is obviously clear that PTTAC (n=3) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 12
In conjunction with Figure 12, embodiment 12 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=4) developer solution of (1) 100 μM: the PTTAC (n=4) taking 27.8mg, be dissolved in the Tris-HCl buffer of the 20mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 5mM containing 60mM in buffer
2, buffer pH be 7.5;
(2) extraction of laten fingerprints: PVDF transfer film is close to the laten fingerprints place on coin, firmly presses transfer film, makes fingerprint fully be transferred on pvdf membrane;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare pvdf membrane immersion step (1) being extracted laten fingerprints on coin, take out after 5s, wash away unnecessary PTTAC developer solution with clear water, dry up;
(4) be extracted the pvdf membrane of laten fingerprints on coin with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 12 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 12 transfer film extracts on coin, and as can be seen from Figure 12, it is obviously clear that PTTAC (n=4) manifests laten fingerprints fingerprint lines as developing agent.
Embodiment 13
In conjunction with Figure 13, embodiment 13 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=5) developer solution of (1) 200 μM: the PTTAC (n=5) taking 57.9mg, be dissolved in the Tris-HCl buffer of the 40mM of 200mL, abundant dissolving mixing is placed on lucifuge place, containing 60mMNaCl in buffer, the pH value of buffer is 8.5;
(2) extraction of laten fingerprints: nitrocellulose filter is close to the laten fingerprints place on A4 paper, firmly presses transfer film, makes fingerprint fully be transferred on nitrocellulose filter;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare nitrocellulose filter immersion step (1) being extracted laten fingerprints on A4 paper, take out after 5s, wash away unnecessary PTTAC developer solution with clear water, naturally dry;
(4) be extracted the nitrocellulose filter of laten fingerprints on A4 paper with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 13 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 13 transfer film extracts on A4 paper, as can be seen from Figure 13, it is obviously clear that PTTAC (n=5) manifests laten fingerprints fingerprint lines as developing agent, and can in permeability object the manifesting of laten fingerprints.
Embodiment 14
In conjunction with Figure 14, embodiment 14 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=6) developer solution of (1) 300 μM: the PTTAC (n=6) taking 90.3mg, be dissolved in the Tris-HCl buffer of the 60mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 15mM containing 20mM in buffer
2, buffer pH value be 7.5;
(2) extraction of laten fingerprints: PVDF transfer film is close to mouse pad, and (mouse pad surface is fabric, fiber grain spacing 1mm, fiber aperture is 0.5mm) on laten fingerprints place, firmly press transfer film, fingerprint be fully transferred on pvdf membrane;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare PVDF transfer film immersion step (1) being extracted laten fingerprints on mouse pad, take out after 5s, wash away unnecessary PTTAC developer solution with clear water, dry up;
(4) be extracted the PVDF transfer film of laten fingerprints on mouse pad with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 14 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 14 transfer film extracts on mouse pad, as can be seen from Figure 14, it is obviously clear that PTTAC (n=6) manifests laten fingerprints fingerprint lines as developing agent, and can on the permeable fiber fabric of larger aperture the manifesting of laten fingerprints.
Embodiment 15
In conjunction with Figure 15, embodiment 15 is described
Perylene derivant manifests the application of laten fingerprints as developing agent:
The preparation of PTTAC (n=8) developer solution of (1) 400 μM: the PTTAC (n=8) taking 129.4mg, be dissolved in the Tris-HCl buffer of the 80mM of 200mL, abundant dissolving mixing is placed on lucifuge place, the MgCl of NaCl and 15mM containing 20mM in buffer
2, buffer pH value be 9.5;
(2) extraction of laten fingerprints: PVDF transfer film is close to the laten fingerprints place on the little skin of arm of human body, pressing transfer film, makes fingerprint fully be transferred on pvdf membrane;
(3) laten fingerprints is manifested: in the PTTAC developer solution prepare PVDF transfer film immersion step (1) being extracted laten fingerprints on skin, take out after 5s, wash away unnecessary PTTAC developer solution with clear water, dry up;
(4) be extracted the PVDF transfer film of laten fingerprints on skin with the ultra violet lamp of 365nm, obtain fingerprint lines clearly.
Figure 15 is the developing latent finger prints fingerprint lines figure out that the embodiment of the present invention 15 transfer film extracts on skin, as can be seen from Figure 15, it is obviously clear that PTTAC (n=8) manifests laten fingerprints fingerprint lines as developing agent, and can on human body skin the manifesting of laten fingerprints.
Claims (11)
1. perylene derivant is as an application for developing agent, it is characterized in that, comprises the following steps:
(1) perylene derivant being dissolved in pH value is be mixed with the perylene derivant developer solution that perylene derivatives concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) object being loaded with laten fingerprints is immersed in 5s ~ 4h in perylene derivant developer solution, obtains the object manifesting laten fingerprints;
(3) under uviol lamp, irradiate the object manifesting laten fingerprints and namely obtain fingerprint stricture of vagina road;
The structural formula of described perylene derivant is:
In formula, n is the integer of 2 ~ 8.
2. a kind of perylene derivant according to claim 1 is as the application of developing agent, it is characterized in that, also comprises, and is rinsed by the object manifesting laten fingerprints described in step (2).
3. a kind of perylene derivant according to claim 2 is as the application of developing agent, it is characterized in that, also comprises, by dry for the object manifesting laten fingerprints after rinsing.
4. a kind of perylene derivant according to claim 1 is as the application of developing agent, it is characterized in that, described buffer is Tris-HCl buffer or phosphate buffer, and concentration is 5mM ~ 100mM.
5. a kind of perylene derivant according to claim 1 is as the application of developing agent, it is characterized in that, be the NaCl of 0 ~ 200mM and concentration is the MgCl of 0 ~ 15mM in described buffer containing concentration
2.
6. perylene derivant is as an application for developing agent, it is characterized in that, comprises the following steps:
(1) perylene derivant being dissolved in pH value is be mixed with the perylene derivant developer solution that perylene derivatives concentration is 1 μM ~ 10mM in the buffer of 6.5 ~ 9.5;
(2) transfer film is placed in the object surface being loaded with laten fingerprints, laten fingerprints extracts on transfer film by pressing transfer film;
(3) transfer film being loaded with laten fingerprints is immersed in 5s ~ 10s in perylene derivant developer solution, obtains the transfer film manifesting laten fingerprints;
(4) under uviol lamp, irradiate the transfer film manifesting laten fingerprints and namely obtain fingerprint stricture of vagina road;
The structural formula of described perylene derivant is:
In formula, n is the integer of 2 ~ 8.
7. a kind of perylene derivant according to claim 6 is as the application of developing agent, it is characterized in that, described transfer film is celluloid transfer film or polyvinylidene fluoride transfer film.
8. a kind of perylene derivant according to claim 6 is as the application of developing agent, it is characterized in that, also comprises, and is rinsed by the transfer film manifesting laten fingerprints described in step (3).
9. a kind of perylene derivant according to claim 8 is as the application of developing agent, it is characterized in that, also comprises, by dry for the transfer film manifesting laten fingerprints after rinsing.
10. a kind of perylene derivant according to claim 6 is as the application of developing agent, it is characterized in that, described buffer is Tris-HCl buffer or phosphate buffer, and concentration is 5mM ~ 100mM.
11. a kind of perylene derivants according to claim 6, as the application of developing agent, is characterized in that, be the NaCl of 0 ~ 200mM and concentration are the MgCl2 of 0 ~ 15mM in described buffer containing concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310161017.0A CN103284728B (en) | 2013-05-04 | 2013-05-04 | Application of perylene derivative |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310161017.0A CN103284728B (en) | 2013-05-04 | 2013-05-04 | Application of perylene derivative |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103284728A CN103284728A (en) | 2013-09-11 |
| CN103284728B true CN103284728B (en) | 2015-07-22 |
Family
ID=49086663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310161017.0A Active CN103284728B (en) | 2013-05-04 | 2013-05-04 | Application of perylene derivative |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103284728B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103800015B (en) * | 2014-02-21 | 2015-08-12 | 北京科技大学 | The method of aobvious laten fingerprints smoked by a kind of wax candle flue dust |
| CN104939839B (en) * | 2015-05-18 | 2017-12-08 | 北京科技大学 | A kind of method for showing latent fingerprint using nitrocellulose filter and water |
| CN105411599B (en) * | 2016-01-13 | 2019-03-26 | 北京科技大学 | A method of showing latent fingerprint using cellulose membrane and aqueous dye solutions |
| CN105975962B (en) * | 2016-06-29 | 2018-11-23 | 西南大学 | A kind of integral method for realizing latent print development and transfer based on the separation of poly-dopamine film interface |
| CN106248647B (en) * | 2016-09-13 | 2019-09-17 | 苏州大学 | Application of the polyparaphenylene Asia ethylene conjugated polymer fluorescent nano material in developing latent finger printss |
| CN106655876B (en) * | 2016-12-06 | 2019-04-02 | 北京科技大学 | Self-initiating alarm and fingerprint location obtain system |
| CN107049331B (en) * | 2017-04-11 | 2019-11-15 | 周婧 | A kind of flexible composite of sandwich structure and its preparation method and application |
| CN110655918A (en) * | 2019-09-27 | 2020-01-07 | 西安交通大学 | Biphase luminous organic fluorescent material and preparation method thereof |
| CN112754477B (en) * | 2019-11-05 | 2021-12-24 | 南京大学 | Latent fingerprint display method based on cesium halide lead perovskite nano material |
| CN110907651B (en) * | 2019-12-12 | 2021-08-20 | 中国科学院长春应用化学研究所 | Fluorescent sensor array for protein distinguishing detection based on perylene derivative probe self-assembly |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100551328C (en) * | 2008-04-30 | 2009-10-21 | 东北师范大学 | Surface-functionalized nanogold particle is used for the latent fingerprint method for visualizing |
| CN102796515B (en) * | 2012-08-09 | 2014-06-18 | 中国科学院长春应用化学研究所 | Fluorescent small-molecule probe for detecting enzyme activity, its preparation method and application |
-
2013
- 2013-05-04 CN CN201310161017.0A patent/CN103284728B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103284728A (en) | 2013-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103284728B (en) | Application of perylene derivative | |
| Ansari et al. | Recent progress of fluorescent materials for fingermarks detection in forensic science and anti-counterfeiting | |
| Xia et al. | Fabrication techniques for microfluidic paper-based analytical devices and their applications for biological testing: A review | |
| Lee et al. | Electrochemical deposition of bismuth on activated graphene-nafion composite for anodic stripping voltammetric determination of trace heavy metals | |
| Jasuja et al. | Fixing latent fingermarks developed by iodine fuming: a new method | |
| AR030650A1 (en) | TEST STRIPS TO DETECT THE PRESENCE OF A REDUCED COFACTOR IN A SAMPLE AND METHODS TO USE THEM | |
| ATE543092T1 (en) | BIOSENSOR | |
| WO2002038724A3 (en) | A method for the detection of viable microorganisms | |
| WO2019242221A1 (en) | Fluorescent sensing film for ph planar optode, preparation method and application | |
| CN103431867A (en) | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein | |
| Zhang et al. | Fingerprint imaging by scanning electrochemical microscopy | |
| Lennard | Fingerprint detection: current capabilities | |
| CN108132214A (en) | A kind of method of heavy metal in quick detection water quality based on super infiltration microchip | |
| CN101881749B (en) | All-solid state dissolved oxygen sensor and preparation method thereof | |
| CN104267013A (en) | Method for detecting potassium dichromate and ascorbic acid by using graphene quantum dot probe | |
| CN109626322A (en) | The simple preparation method and SERS of Nano pointed cone shaped polymer array are applied | |
| CN104034711A (en) | Method for detecting potassium dichromate by utilizing graphene quantum dot probe | |
| KR20190024686A (en) | Hybrid nano probe based on polyhedral oligomeric silsesquioxane and sensor comprising the probe | |
| WO2019015448A1 (en) | Detection membrane for detecting cadmium ions in body of water, preparation method therefor, and application thereof | |
| Prabowo et al. | Environmentally friendly and biodegradable components for biosensors | |
| CN109517672A (en) | A kind of Roche Electrogenerated chemiluminescent immunoassay instrument cleaning solution and preparation method thereof | |
| CN105286877B (en) | It is a kind of in the positive and negative method for showing latent fingerprint of different hydrophilic and hydrophobic material surfaces | |
| CN111100184B (en) | Fluorescent probe for detecting superoxide anion free radicals in peroxisome and application thereof | |
| MY118924A (en) | A process for measuring concentration of nonionic surfactants in an aqueous alkaline solutions | |
| Mayer et al. | The Use of Ortho-phthalaldehyde for Superior Fluorescent Visualization of Latent Fingerprints |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |