Summary of the invention
The present invention is directed to above weak point, provide a planting fruit-trees, forest trunk disease therapeutic agent, solve that existing preparation residual hazard is comparatively large, the drug effect phase is short, healing slow, the easy shortcoming such as recurrence, be a kind of there is protection, new medicament that treatment, growth-promoting are integrated.
The technical solution adopted for the present invention to solve the technical problems is: a planting fruit-trees, forest trunk disease therapeutic agent, and its composition is by mass percentage:
Tebuconazole 0.5% ~ 5%,
Thiophanate-methyl 1% ~ 50%,
Dispersant 1% ~ 3%,
Wetting agent 1% ~ 3%,
Antifreezing agent 1% ~ 4%,
Film forming agent polyvinyl alcohol 3% ~ 20%,
Colouring agent gentian violet 0.1% ~ 2.0%,
Bleeding agent 0.5% ~ 2%,
Plant growth regulating hormone methyl α-naphthyl acetate 0.002% ~ 0.008%, gibberellin 0.0002% ~ 0.002%,
Water 40% ~ 90%.
Described dispersant selects alkylphenol polyoxyethylene phosphate.
Alkylphenol polyoxyethylene selected by described wetting agent.
Glycerine selected by described antifreezing agent.
Peregal selected by described bleeding agent.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
The invention has the beneficial effects as follows:
In the present invention, Tebuconazole belongs to Novel triazole bactericide, has the advantages that bactericidal activity is high, fungicidal spectrum is wide; Thiophanate-methyl inhales low toxicity bactericide in a kind of broad spectrum activity, has preventive and therapeutic action to various crop disease; The solution of gentian violet is commonly called as gentian violet, belongs to basic-dyeable fibre class Cidex-7 thing, has the feature of surface disinfection sterilization, convergence wound; Methyl α-naphthyl acetate, gibberellin are high activity auxins plant growth regulator, the feature of the scab healing that has activating cell, is beneficial to Callus formation, promotes; Polyvinyl alcohol is a kind of conventional safety film forming agent, has the feature of medicament sustained-release synergistic, ventilative printing opacity, isolation diseases prevention, closed water conservation.
The various technology such as infiltration sterilization, surface sterilization, cell activation growth, sealing water conservation combine together by the present invention, have the double effects of physical isolation diseases prevention and infiltration antimicrobial therapy.It is strong that rear film forming smeared by this therapeutic agent, interior sorptivety thoroughly, low toxic and environment-friendly, resistance of rainwater washing against, the drug effect phase is long, and both cause of disease invasion capable of blocking, killed and suppress cause of disease growth, again can Quick seal water conservation, activating cell, promotion wound healing; Several technology are combined together, can reach safe and efficient, radical rate is high, the effect that recurrence rate is low, there is good result for fruit tree forest trunk diseases such as control apple tree, cherry tree, pear tree, peach, Chinese chestnut, walnut, jujube tree, Malus spectabilis, willow, willows, also can be used as the sealing compound of fruit tree forest grafting beta pruning.
Embodiment
Describe the present invention below in conjunction with specific embodiment:
Embodiment 1
Its composition of described therapeutic agent is by mass percentage:
Tebuconazole 2.3978%
Thiophanate-methyl 1%
Dispersant (alkylphenol polyoxyethylene phosphate) 1%,
Wetting agent (alkylphenol polyoxyethylene) 1%,
Antifreezing agent (glycerine) 1%,
Film forming agent (polyvinyl alcohol) 3%,
Colouring agent (gentian violet) 0.1%,
Bleeding agent (peregal) 0.5%,
Plant growth regulating hormone (methyl α-naphthyl acetate 0.002%, gibberellin 0.0002%),
Water 90%.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
Embodiment 2
Its composition of described therapeutic agent is by mass percentage:
Tebuconazole 5%,
Thiophanate-methyl 30%,
Dispersant (alkylphenol polyoxyethylene phosphate) 3%,
Wetting agent (alkylphenol polyoxyethylene) 3%,
Antifreezing agent (glycerine) 4%,
Film forming agent (polyvinyl alcohol) 10.99%,
Colouring agent (gentian violet) 2.0%,
Bleeding agent (peregal) 2%,
Plant growth regulating hormone (methyl α-naphthyl acetate 0.008%, gibberellin 0.002%),
Water 40%.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
Embodiment 3
Its composition of described therapeutic agent is by mass percentage:
Tebuconazole 0.5%,
Thiophanate-methyl 15%,
Dispersant (alkylphenol polyoxyethylene phosphate) 2%,
Wetting agent (alkylphenol polyoxyethylene) 2%,
Antifreezing agent (glycerine) 2.5%,
Film forming agent (polyvinyl alcohol) 20%,
Colouring agent (gentian violet) 1.5%,
Bleeding agent (peregal) 1.494%,
Plant growth regulating hormone (methyl α-naphthyl acetate 0.005%, gibberellin 0.001%),
Water 55%.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
Embodiment 4
Its composition of described therapeutic agent is by mass percentage:
Tebuconazole 2.5%,
Thiophanate-methyl 13.5%,
Dispersant (alkylphenol polyoxyethylene phosphate) 1.5%,
Wetting agent (alkylphenol polyoxyethylene) 1.5%,
Antifreezing agent (glycerine) 2%,
Film forming agent (polyvinyl alcohol) 11.5%,
Colouring agent (gentian violet) 1.2%,
Bleeding agent (peregal) 1.295%,
Plant growth regulating hormone (methyl α-naphthyl acetate 0.004%, gibberellin 0.001%),
Water 65%.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
Embodiment 5
Its composition of described therapeutic agent is by mass percentage:
Tebuconazole 5%,
Thiophanate-methyl 1%,
Dispersant (alkylphenol polyoxyethylene phosphate) 3%,
Wetting agent (alkylphenol polyoxyethylene) 1%,
Antifreezing agent (glycerine) 4%,
Film forming agent (polyvinyl alcohol) 3%,
Colouring agent (gentian violet) 1.5%,
Bleeding agent (peregal) 1.493%,
Plant growth regulating hormone (methyl α-naphthyl acetate 0.006%, gibberellin 0.001%),
Water 80%.
The compound method of this therapeutic agent comprises following steps:
A () adds a small amount of water and mixes with polyvinyl alcohol, glycerine, added by remaining water again, be heated to 100 DEG C, naturally cool to 50 ~ 70 DEG C after being heated to dissolve;
B () is by Tebuconazole, thiophanate-methyl, gentian violet, gibberellin, methyl α-naphthyl acetate and alkylphenol polyoxyethylene phosphate, alkylphenol polyoxyethylene, peregal, add suitable quantity of water, stirring and evenly mixing, more than 4 hours are ground by grinder, then add in the solution of step (a) the agglutination paste that stirs, fruit tree of the present invention, forest trunk disease therapeutic agent can be obtained.
Application example one
1. Toxicity Determination
Mycelial growth rate method is adopted to measure 5 kinds of bactericide to the inhibitory action of rotten pathogenic bacteria.By rotten pathogenic bacteria 26 DEG C of preculture 5d on PDA medium, bacterium cake is got same circumferentially the beating near colony edge with the card punch of diameter 7mm, be inoculated on the culture medium flat plate containing different agents concentration, every ware a slice, be placed in 26 DEG C of insulating boxs and cultivate 4d, measure the colony growth diameter of each process by right-angled intersection method, take clear water as contrast, every concentration process repeats 4 times.Measure the radial linear amount of growth of bacterium colony, determine the inhibiting rate of medicament to colony growth.Suppress the linear regression analysis between probable value and drug concentration logarithm value by mycelial growth, obtain each medicament to concentration (EC in effective suppression of bacterial strain
50value).
Test data carries out statistical analysis by Microsoft Excel 2003, DPS data processing work platform.
Developing restraint rate (%)=[(contrast colony diameter-process colony diameter)/(contrast colony diameter-bacterium cake diameter)] × 100
Medicament |
Virulence regression equation |
r |
EC
50(mg/Kg)
|
Trunk disease therapeutic agent |
y=10.1764+2.8449x |
0.9986 |
0.02617 |
43% tebuconazole suspension concentrates |
y=15.4176+6.9593x |
0.9951 |
0.03215 |
70% thiophanate-methyl wetting powder |
y=0.6248+0.8274x |
0.9980 |
20.1304 |
2.12% copper humate aqua |
y=3.7333+1.1415x |
0.9584 |
12.8700 |
45% lime sulfur wetting powder |
y=3.2957+1.0058x |
0.9876 |
49.4900 |
Toxicity test result sees the above table, trunk disease therapeutic agent EC
50minimum, Indoor antibacterial activity is the highest, apparently higher than bactericide such as 2.12% copper humate aqua, 45% lime sulfur wetting powders; And trunk disease therapeutic agent bacteriostatic activity is also higher than the effect that 43% tebuconazole suspension concentrates, 70% thiophanate-methyl wetting powder are used alone, show that this formula has synergistic effect.
2. field rot therapeutic test
Test is carried out in Zhang An village, An Jiazhuan town, Feicheng, Shandong, and test tree is that 16 years raw gold are handsome, and spacing in the rows × line-spacing=3 × 4m, rich water quality management level is general, extensive management, and tree vigo(u)r is weak, and the trunk disease such as rot, ring spot occurs heavier.Design 6 test process: (1) trunk disease therapeutic agent; (2) 43% tebuconazole suspension concentrates 200 times; (3) 3% thiophanate-methyl pastes; (4) 2.12% copper humate aquas; (5) 40% asomate wetting powders 50 times; (6) blank, only removes disease scar without chemicals treatment.Every 20 strains trees are a community, repeat 4 times, and selection close, the sick scar position of tree vigo(u)r, every community and close 10 the sick scars of sick scar size mark.Therefore often process and mark 40 pieces of sick scars altogether.
Test and carry out on March 10th, 2011, medication after scab cleaning, adopt to produce upper conventional to scrape therapy, namely first with scraper, rot disease portion scraping is also exceeded disease portion slough 0.5-1cm in clear limit, sick scar otch requires vertical stubble, fusiformis, and then with hairbrush, liquid medicine painting disease portion exceeded otch 2-3cm.
When early winter on December 3rd, 2011 and the early spring on the 15th March in 2012, respectively investigate a scab recurrence, the sick scar quantity of record recurrence, record the maximum healing width (mm) of disease scar with vernier caliper measurement simultaneously.
Drug effect statistical analysis technique: by following formulae discovery pharmacy control efficacy.
Recur disease scar number
A. the incidence of disease (%)=------------× 100 (is answered)
Investigate total sick scar number
The multiple incidence of disease of the multiple incidence of disease-process of contrast
B. control efficiency (%)=------------------× 100
The multiple incidence of disease of contrast
Field rot therapeutic test result sees the above table.From table, trunk disease therapeutic agent field control effect is best, and cure rate, more than 90.0%, is significantly better than other each conventional treatment, and wound healing is fast, smooth.
Application example two
Field apple tree Trunk canker therapeutic test
Test Wu Cun garden, Qufu City Wu villages and small towns, Jining City in Shandong Province is level land, sandy loam, and cultivation management condition is general, around many apple-plantings garden.Test tree is 18 years raw golden marshall apples, and tree vigo(u)r is weak, spacing in the rows × line-spacing=3 × 4m, and between individual plant, tree vigo(u)r is close, and the sick knurl of limb is more.Design 5 test process: (1) trunk disease therapeutic agent; (2) 43% tebuconazole suspension concentrates 200 times; (3) 3% thiophanate-methyl pastes; (4) 5 Baume degrees lime sulfurs; (5) blank, only removes disease scar without chemicals treatment.Every 20 strains trees are a community, repeat 4 times, and selection close, the sick scar position of tree vigo(u)r, every community and close 10 the sick scars of sick scar size mark.
Test and carry out on November 30th, 2011, with hairbrush uniform application liquid on 2 years above branches of life, until trunk base portion, average every strain is coated with liquid about about 500 milliliters, before coating, set every orientation, orientation in by all directions in two strains of every community and select the raw branches of 5 3-4, paint the branch section having certain sick knurl of demarcation 60-100 square centimeters thereon respectively.
Respectively investigate once with on April 30th, 2012 before dispenser, investigate twice altogether.Investigate each community and respectively mark sick knurl quantity in area, with knurl number sick before coating for radix, add up disease knurl growth rate, calculate control efficiency.
Total knurl number before total knurl number-medicine after medicine
Sick knurl growth rate (%)=------------------------------------× 100
Total knurl number before medicine
The sick knurl growth rate of the sick knurl growth rate-treatment region in check plot
Control efficiency (%)=-------------------------------------× 100
The sick knurl growth rate in check plot
Trunk disease therapeutic agent control eyespot in apple limb test initial data statistical form (2011)
Trunk disease therapeutic agent control eyespot in apple limb result of the test sees the above table.
From table, trunk disease therapeutic agent field control effect is best, cure rate is 85.18%, and be significantly better than other process, the mode of smearing with limb on producing uses trunk disease therapeutic agent, raw material is easy to get, moderate cost, preparation is convenient, and bactericidal effect is good, environmental pollution is little, is the preferred agents of control eyespot in apple limb.
Application example three
Field cherry tree gummosis therapeutic test
Test is carried out in cherry planting base, the Tai'an summer Zhang Zhenzhu family village, and test tree is 10 years raw red lights, chooses tree vigo(u)r is suitable, the state of an illness is close, control measures are consistent plant as trying material.Design 5 test process: (1) trunk disease therapeutic agent; (2) 43% tebuconazole suspension concentrates 200 times; (3) 3% thiophanate-methyl pastes; (4) 5 Baume degrees lime sulfurs; (5) blank, only removes colloid without chemicals treatment.Every 4 strain trees are a community, repeat 3 times, randomized arrangement.
Remove disease portion dead skin, breach and blob of viscose on April 25th, 2009 (rainy season start before), with scraper at sick cortex place cut in length and breadth, then smear trunk disease therapeutic agent with scrub-brush, scope exceeds disease portion 3 ~ 5cm.Interval is coated with once for 7 days again.
Every community is coated with 20 gummosis scabs, and often process amounts to 60.Be coated with latter 60 days and observe and cure (no longer gummosis) number and gummosis place healing state (stop gummosis, dead tissue is replaced by cambium) around, using cure rate as indicator-specific statistics control efficiency.
Field gummosis therapeutic test result
Process |
Process gummosis is counted |
Cure number |
Cure rate (%) |
Trunk disease therapeutic agent |
60 |
54 |
90.0 |
43% tebuconazole suspension concentrates 200 times |
60 |
52 |
86.7 |
3% thiophanate-methyl paste |
60 |
45 |
75.0 |
5 Baume degrees lime sulfurs |
60 |
42 |
70.0 |
Contrast |
60 |
6 |
10.0 |
Field cherry tree gummosis therapeutic test result sees the above table.
Trunk disease therapeutic agent field control effect is best, and cure rate, 90.0%, is significantly better than other each conventional treatment, and wound healing is fast, smooth.
Application example four
Field Valsa sordida therapeutic test
Test is carried out in Daiyue District summer Zhang Zhenxi peach garden village, Tai'an, Shandong Province, and test tree is 8 years raw fast-growing 107 willows, planting density 760 strains/hm
2, soil property is loam, and water and fertilizer management is medium, selects the willow that lesion area is close to try plant as supplying.
Design 5 test process: (1) trunk disease therapeutic agent; (2) 43% tebuconazole suspension concentrates 200 times; (3) 3% thiophanate-methyl pastes; (4) 5 Baume degrees lime sulfurs; (5) blank, equivalent clear water.Every 20 strains trees are a community, repeat 4 times, and every community selects close, the sick scar position of tree vigo(u)r and close 10 the sick scars of sick scar size to mark, and often processes mark 40 pieces of sick scars altogether.
Test on April 15th, 2010, scrape when controlling and diseased tissues is all scraped totally, and clear limit exceeds scab border 1cm, and then with hairbrush, liquid medicine painting disease portion is exceeded otch 2-3cm.
A scab recurrence is respectively investigated, the sick scar quantity of record recurrence, statistics recurrence rate when early winter on December 5th, 2010 and the early spring on the 18th March in 2011.
Drug effect statistical analysis technique: by following formulae discovery pharmacy control efficacy.
Recur disease scar number
A. the incidence of disease (%)=------------× 100 (is answered)
Investigate total sick scar number
The multiple incidence of disease of the multiple incidence of disease-process of contrast
B. control efficiency (%)=------------------× 100
The multiple incidence of disease of contrast
field Valsa sordida result of the test
Field Valsa sordida therapeutic test result sees the above table.As can be seen from table, each medicament all has better result for the treatment of to Valsa sordida, and wherein trunk disease therapeutic agent field control effect is best, first time on December 5th, 2010 investigation cure rate is more than 87.7%, significantly be better than other each conventional treatment, and wound healing is fast, smooth.
The trunk disease of fruit tree, forest has a variety of, compares and common are rot, ring spot, gummosis etc., and the using method of the present invention on other trunk diseases is with 3 kinds of above citing, through test, respond well.