CN103278497B - Copper ion colorimetry detecting kit and detecting method thereof - Google Patents
Copper ion colorimetry detecting kit and detecting method thereof Download PDFInfo
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- CN103278497B CN103278497B CN201310178095.1A CN201310178095A CN103278497B CN 103278497 B CN103278497 B CN 103278497B CN 201310178095 A CN201310178095 A CN 201310178095A CN 103278497 B CN103278497 B CN 103278497B
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 229910001431 copper ion Inorganic materials 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000004737 colorimetric analysis Methods 0.000 title abstract description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 52
- BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 239000000243 solution Substances 0.000 claims description 71
- XQSBLCWFZRTIEO-UHFFFAOYSA-N hexadecan-1-amine;hydrobromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[NH3+] XQSBLCWFZRTIEO-UHFFFAOYSA-N 0.000 claims description 60
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 18
- 229910052802 copper Inorganic materials 0.000 claims description 18
- 239000010949 copper Substances 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000010790 dilution Methods 0.000 claims description 9
- 239000012895 dilution Substances 0.000 claims description 9
- 238000001556 precipitation Methods 0.000 claims description 8
- 239000011435 rock Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 abstract 5
- 230000007613 environmental effect Effects 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 1
- 230000009897 systematic effect Effects 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 description 8
- 238000005530 etching Methods 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 238000000862 absorption spectrum Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 4
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- VMQMZMRVKUZKQL-UHFFFAOYSA-N Cu+ Chemical compound [Cu+] VMQMZMRVKUZKQL-UHFFFAOYSA-N 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 2
- 229910001961 silver nitrate Inorganic materials 0.000 description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 description 2
- 239000012279 sodium borohydride Substances 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- KZNMRPQBBZBTSW-UHFFFAOYSA-N [Au]=O Chemical compound [Au]=O KZNMRPQBBZBTSW-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000012455 bioassay technique Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 229910001922 gold oxide Inorganic materials 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
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- 238000000918 plasma mass spectrometry Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention relates to the technical field of environmental and biological analysis, and in particular relates to a copper ion colorimetry detecting kit and a detecting method thereof. The kit comprises a hydrobromic acid solution, a gold nanorod solution containing cetyl trimethyl ammonium bromide (CTAB), a standard colorimetric card C and a colorimetric tube, wherein the gold nanorod solution containing the CTAB is formed by dissolving gold nanorods in a CTAB solution under the condition that the concentration of the gold nanorods is within 0.5 to 10nM and the concentration of the CTAB is with 0.01 to 100mM; and the concentration of hydrobromic acid in the hydrobromic acid solution is within 0.05 to 5.0M. The kit for detecting copper ions has the advantages of being high in sensitivity, good in selectivity, easy to operate, easy in naked-eye observation and the like. Under the debugging of a systematic experiment condition, the detection on the copper ions in environmental and biological samples can be realized.
Description
Technical field
The present invention relates to environment and bioassay technique field, specifically a kind of copper ion colorimetric determination kit and detection method thereof.
Background technology
Copper ion is one of indispensable element of wanting of body weight for humans, and he plays an important role in the function of many cellular enzymes and protein.But will cause copper metabolic disorder when the accumulation of copper ion in human body exceedes certain limit, thus bring very large harm to human body, such as, copper metabolic disorder can increase the probability of suffering from senile dementia and Parkinson's disease.In water body environment, because copper ion can not decompose, thus have biological concentration and biological amplification in vivo, thus make for the higher biology of food chain, in its body, content of copper ion also increase at double, also larger to its harm caused.As the mankind on food chain top, when edible these contain the heavy metal ion aqueous bio of high concentration, the harm be subject to also is maximum.Thus, the detection of the copper ion in environment and biological sample is necessary.
At present, Atomic absorption is mainly contained to the detection means of copper ion, atomic emissions, inductivity coupled plasma mass spectrometry coupling technique etc.But these technology all need large-scale instrument, expensive, on-the-spot in situ detection and Technique Popularizing can not be realized! Relatively above detection technique, colorimetric analysis advantage is that its naked eyes are easy to observation, is very suitable for on-the-spot detecting in real time, simple to operate and low price.Golden nanometer particle has very large molar extinction coefficient because of its surface plasmon absorption, and thus it applies to have obvious advantage in colorimetric analysis.The present invention utilizes copper ions catalyze to etch gold nanorods colorimetric determination copper ion, and have highly sensitive, selectivity is good, simple to operate, and speed is fast, and naked eyes are easily observed, and is applicable to the advantages such as on-the-spot detection in real time.And the domestic kit based on copper ions catalyze etching gold nanorods colorimetric determination copper ion have not been reported at present.
Summary of the invention
The object of the invention is to provide a kind of copper ion colorimetric determination kit and detection method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of copper ion colorimetric determination kit, is characterized in that: kit comprises hydrobromic acid solution, gold nanorods solution, standard color comparison card C and color comparison tube containing cetyl ammonium bromide (CTAB);
Described is that gold nanorods is dissolved in CTAB solution containing cetyl ammonium bromide (CTAB) gold nanorods solution, and wherein the concentration of nanometer rods is 0.5-10nM, CTAB concentration is 0.01-100mM;
In described hydrobromic acid solution, hydrobromic acid concentration is 0.05-5.0M.
Further, preparation containing CTAB gold nanorods solution is that gold nanorods is turned the/centrifugal 5-30 minute of min at 7000-10000, collecting precipitation dilute with water, / centrifugal the 5-30 minute of min is turned with 6000-9000 again after dilution, precipitation uses cetyl ammonium bromide (CTAB) solution dilution of 0.01-100mM mM again, stand-by.
800 μ L hydrobromic acid solutions are added in color comparison tube by the method for the detection copper ion of copper ion colorimetric determination kit, add 5-50 μ L testing sample and rock mixing, add the gold nanorods 200 μ L containing CTAB again, shake up after mixing; Then at 40-90 DEG C of temperature, hatch 5-30 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card C and determine content of copper ion.
The preparation of described standard color comparison card C is, gets 0-1mM variable concentrations copper standard color range solution and is positioned over respectively in color comparison tube, add hydrobromic acid solution and rock mixing, add the gold nanorods containing CTAB again, shake up after mixing; Then at 50-90 DEG C of temperature, hatch 5-40 minute, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form copper standard color comparison card C with the picture by color gradient.
Described 0-1mM variable concentrations copper standard color range solution concentration is respectively 0,10,50,100,500,1000,10
4, 10
5, 10
6nM.
In described hydrobromic acid solution, hydrobromic acid concentration is 0.5M.
Described is that gold nanorods is dissolved in cetyl ammonium bromide (CTAB) solution containing cetyl ammonium bromide (CTAB) gold nanorods solution, and wherein the concentration of nanometer rods is 2.3nM, CTAB concentration is 1.0mM.
Principle (see figure 1) of the present invention is in an acidic solution, copper ion gold oxide nanometer rods and generate cuprous ion, cuprous ion can be copper ion by the dissolved oxygen DO rapid oxidation in solution again, thus form circulation catalysis etching gold nanorods (see figure 2), make its absorption spectrum change (see figure 3) changing along with obvious color, this change and the linear (see figure 5) of copper ion concentration simultaneously.
The advantage that the present invention has: the present invention utilizes copper ions catalyze to etch gold nanorods colorimetric determination copper ion, have highly sensitive (detect and be limited to 0.5nM), selectivity good (see Fig. 4), simple to operate, speed is fast, naked eyes are easily observed, and are applicable to the advantages such as on-the-spot detection in real time.
Accompanying drawing explanation
Fig. 1 is the principle of copper ions catalyze provided by the invention etching gold nanorods.
Images of transmissive electron microscope before and after the copper ions catalyze etching gold nanorods that Fig. 2 provides for the embodiment of the present invention; Wherein, gold nanorods (left side), afterwards (centre is low concentration, and the right side is high concentration) before copper ions catalyze etching.
Abosrption spectrogram after copper ion (concentration from low to high) the catalysis etching gold nanorods that Fig. 3 provides for the embodiment of the present invention.
The selectivity experimental result of the detection copper ion that Fig. 4 provides for the embodiment of the present invention and corresponding photo.
Fig. 5 for the embodiment of the present invention provide copper ion standard color comparison card C.
The detection copper ion process flow diagram that Fig. 6 provides for the embodiment of the present invention.
Fig. 7 is that the present invention detects copper ion typical curve
Embodiment
Embodiment 1: copper in drinking water ion detection
Kit comprises hydrobromic acid solution, gold nanorods solution, standard color comparison card C and color comparison tube containing cetyl ammonium bromide (CTAB);
Described hydrobromic acid preparation: the pure hydrobromic acid of the analysis by 38% dilutes and obtains, and concentration is 0.5M hydrobromic acid solution.
Preparation containing cetyl ammonium bromide (CTAB) gold nanorods solution:
1) gold nanorods obtains as follows:
Synthesis gold seeds liquid: get 50 μ L gold chloride (50mM) solution and shake up a few second to 7.7mL containing in cetyl ammonium bromide (CTAB) solution of 0.1M, add 600 μ L ice sodium borohydride (0.01M) accelerate rock a few minutes, color is stopped to during purplish grey by glassy yellow, under 26 degrees Celsius of environment, leave standstill after 2 hours and use.
Nanometer rods is synthesized, gold chloride (50mM) solution is joined in cetyl ammonium bromide (CTAB) solution of 0.1M and stir and evenly mix, then add silver nitrate (0.01M) solution and the mixing of ascorbic acid (0.1M) solution again, the gold seeds solution liquid adding above-mentioned synthesis when solution is become colorless by yellowish-brown under stirring has the five colors to gradually become blue-green to solution colour, stop stirring, leave standstill 20 hours, namely obtain gold nanorods.From transmission electron microscope wherein gold nanorods length-diameter ratio be about 2:1(and see Fig. 2 (left side)), the radial absorption peak of uv-visible absorption spectra figure is shown in figure tri-abosrption spectrogram 0 at 670nm(), according to langbobier law, the concentration estimating nanometer rods is 2.3nM.
2) containing cetyl ammonium bromide (CTAB) gold nanorods solution: by gold nanorods (its maximum absorption wavelength is at 670nM) at 8000 turns/min centrifugal 15 minutes, collecting precipitation dilute with water, after dilution with 7000 turns/min centrifugal 15 minutes again, precipitation uses cetyl ammonium bromide (CTAB) solution dilution of 1mM again, to obtain final product.
The preparation of standard color comparison card C is, getting concentration is 0,10,50,100,500,1000,10
4, 10
5, 10
6the copper standard color range solution 10 μ L of nM joins 800 μ L hydrobromic acid solutions respectively and rocks mixing, adds the gold nanorods containing CTAB again, shake up after mixing; Then at 75 DEG C of temperature, hatch 20 minutes, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form copper standard color comparison card C(with the picture by color gradient and see Fig. 5), corresponding uv-visible absorption spectra figure can be gathered simultaneously, and according to peak shift production standard curve (as Fig. 7).
I.e. kit:
Hydrobromic acid solution, concentration is 0.5M;
Color comparison tube, 1.5mL;
Glass scales suction pipe;
Copper standard color range solution;
Copper standard color comparison card.
Copper in drinking water ion detection:
(1) pipette 800 μ L hydrobromic acid solutions in 1.5mL color comparison tube, add water body example 10 μ L to be measured, after rocking mixing, add containing CTAB gold nanorods 200 μ L, shake up;
(2) at 75 DEG C of temperature, 20 minutes are hatched.
(3) color change is observed, color comparison tube and standard color comparison card C are compared, determine the concentration range of copper ion, respective absorption spectrogram can be measured simultaneously, determined the concrete content of copper ion by typical curve (Fig. 7), experimental result shows this method testing result and ICP-MS measurement result basically identical (table 1).
Table 1 this method and ICP-MS are to actual water sample copper ion testing result
Embodiment 2: in biological sample, copper ion detects
Kit comprises hydrobromic acid solution, gold nanorods solution, standard color comparison card C and color comparison tube containing cetyl ammonium bromide (CTAB);
Described hydrobromic acid preparation: the pure hydrobromic acid of the analysis by 38% dilutes and obtains, and concentration is 0.5M hydrobromic acid solution.
Preparation containing cetyl ammonium bromide (CTAB) gold nanorods solution:
1) gold nanorods obtains as follows:
Synthesis gold seeds liquid: get 50 μ L gold chloride (50mM) solution and shake up a few second to 7.7mL containing in cetyl ammonium bromide (CTAB) solution of 0.1M, add 600 μ L ice sodium borohydride (0.01M) accelerate rock a few minutes, color is stopped to during purplish grey by glassy yellow, under 26 degrees Celsius of environment, leave standstill after 2 hours and use
Nanometer rods is synthesized, gold chloride (50mM) solution is joined in cetyl ammonium bromide (CTAB) solution of 0.1M and stir and evenly mix, then add silver nitrate (0.01M) solution and the mixing of ascorbic acid (0.1M) solution again, the gold seeds solution liquid adding above-mentioned synthesis when solution is become colorless by yellowish-brown under stirring has the five colors to gradually become blue-green to solution colour, stop stirring, leave standstill 20 hours, namely obtain gold nanorods.From transmission electron microscope wherein gold nanorods length-diameter ratio be about 2:1(and see Fig. 2 (left side)), the radial absorption peak of uv-visible absorption spectra figure is shown in figure tri-abosrption spectrogram 0 at 670nM(), according to langbobier law, the concentration estimating nanometer rods is 2.3nM.
2) containing cetyl ammonium bromide (CTAB) gold nanorods solution: by gold nanorods (its maximum absorption wavelength is at 670nM) at 8000 turns/min centrifugal 15 minutes, collecting precipitation dilute with water, after dilution with 7000 turns/min centrifugal 15 minutes again, precipitation uses cetyl ammonium bromide (CTAB) solution dilution of 1mM again, to obtain final product.
The preparation of standard color comparison card C is, getting concentration is 0,10,50,100,500,1000,10
4, 10
5, 10
6the copper standard color range solution 10 μ L of nM joins 800 μ L hydrobromic acid solutions respectively and rocks mixing, adds the gold nanorods containing CTAB again, shake up after mixing; Then at 75 DEG C of temperature, hatch 20 minutes, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form copper standard color comparison card C(with the picture by color gradient and see Fig. 5).
I.e. kit:
Hydrobromic acid solution, concentration is 0.5M;
Color comparison tube, 1.5mL;
Glass scales suction pipe;
Copper standard color range solution;
Copper standard color comparison card.
In biological sample, copper ion detects (for sea cowry):
(1) sample pretreatment: shell is removed in the biological sample fetched cleaning, obtains the active component that need detect stand-by, then clean three times with intermediate water, then refrigerator freezing Cheng Bing is put into, and then put into freeze-dryer removal moisture, after drying, then in mortar, pulverize is stand-by.
(2) Specimen eliminating: get 0.3 gram of above-mentioned sample, join in the red fuming nitric acid (RFNA) of 10mL, clear up 6 hours at 150 DEG C, finally by solution dilution to 50mL.
(3) get 800 microlitre hydrobromic acid solutions in 1.5mL centrifuge tube, add and above-mentionedly clear up sample 10 μ L, after rocking mixing, to add in reagent bottle B containing CTAB gold nanorods 200 μ L, shake up.
(4) at 75 DEG C of temperature, 20 minutes are hatched.
(5) by naked eyes, color comparison tube and standard color comparison card C are compared, determine the concentration range of copper ion, respective absorption spectrogram can be measured simultaneously, determined the concrete content of copper ion by typical curve (Fig. 7), experimental result shows this method testing result and ICP-MS measurement result basically identical (table 2).
Table 2 this method testing result and ICP-MS measurement result
Claims (6)
1. a copper ion colorimetric determination kit, is characterized in that: kit comprises hydrobromic acid solution, gold nanorods solution, standard color comparison card C and color comparison tube containing cetyl ammonium bromide (CTAB);
Described is that gold nanorods is dissolved in CTAB solution containing cetyl ammonium bromide (CTAB) gold nanorods solution, and wherein the concentration of nanometer rods is 0.5-10nM, CTAB concentration is 0.01-100mM;
In described hydrobromic acid solution, hydrobromic acid concentration is 0.05-5.0M;
Preparation containing CTAB gold nanorods solution is that gold nanorods is turned the/centrifugal 5-30 minute of min at 7000-10000, collecting precipitation dilute with water, / centrifugal the 5-30 minute of min is turned with 6000-9000 again after dilution, precipitation uses cetyl ammonium bromide (CTAB) solution dilution of 0.01-100mM again, stand-by.
2. the method for the detection copper ion of a copper ion colorimetric determination kit according to claim 1, it is characterized in that: 800 μ L hydrobromic acid solutions are added in color comparison tube, add 5-50 μ L testing sample and rock mixing, add the gold nanorods 200 μ L containing CTAB after mixing again, shake up; Then at 40-90 DEG C of temperature, hatch 5-30 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card C and determine content of copper ion.
3. by the method for the detection copper ion of copper ion colorimetric determination kit according to claim 2, it is characterized in that: the preparation of described standard color comparison card C is, get 0-1mM variable concentrations copper standard color range solution to be positioned over respectively in color comparison tube, add hydrobromic acid solution and rock mixing, add the gold nanorods containing CTAB after mixing again, shake up; Then at 50-90 DEG C of temperature, hatch 5-40 minute, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form copper standard color comparison card C with the picture by color gradient.
4., by the method for the detection copper ion of copper ion colorimetric determination kit according to claim 3, it is characterized in that: described 0-1mM variable concentrations copper standard color range solution concentration is respectively 0,10,50,100,500,1000,10
4, 10
5, 10
6nM.
5., by the method for the detection copper ion of the copper ion colorimetric determination kit described in Claims 2 or 3, it is characterized in that: in described hydrobromic acid solution, hydrobromic acid concentration is 0.5M.
6. by the method for the detection copper ion of the copper ion colorimetric determination kit described in Claims 2 or 3, it is characterized in that: described is that gold nanorods is dissolved in cetyl ammonium bromide (CTAB) solution containing cetyl ammonium bromide (CTAB) gold nanorods solution, wherein the concentration of nanometer rods is 2.3nM, CTAB concentration is 1.0mM.
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| CN105277540B (en) * | 2015-02-05 | 2018-03-23 | 温州医科大学 | Copper ion field fast detection method in a kind of water for eliminating reagent blank influence |
| CN105466920A (en) * | 2015-11-20 | 2016-04-06 | 鲁翌 | A rapid antithrombin III detecting kit based on interaction between thrombin and a chromogenic substrate and a detecting method thereof |
| CN106198522B (en) * | 2016-07-05 | 2019-02-22 | 河南城建学院 | A kind of fast detection kit for copper ions and its detection method |
| CN106568773B (en) * | 2016-11-22 | 2020-02-21 | 中国科学院烟台海岸带研究所 | Manganese ion colorimetric detection kit and detection method thereof |
| CN107064134A (en) * | 2017-06-14 | 2017-08-18 | 大连理工大学 | A kind of analysis method for organophosphorus pesticide Glassless half-quantitative detection |
| US11371978B1 (en) | 2021-06-23 | 2022-06-28 | Mks Vision, Llc | System and method for detecting lead in water |
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