CN103274835B - A kind of complex microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof - Google Patents
A kind of complex microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof Download PDFInfo
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- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 title claims abstract description 42
- 235000010591 Appio Nutrition 0.000 title claims abstract description 42
- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 239000003337 fertilizer Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 244000101724 Apium graveolens Dulce Group Species 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 67
- 230000004151 fermentation Effects 0.000 claims abstract description 67
- 240000007087 Apium graveolens Species 0.000 claims abstract description 41
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 40
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 24
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 24
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 24
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 17
- 210000003608 fece Anatomy 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000002893 slag Substances 0.000 claims abstract description 11
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 10
- 240000008042 Zea mays Species 0.000 claims abstract description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 10
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- 239000010871 livestock manure Substances 0.000 claims abstract description 10
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 10
- 239000010902 straw Substances 0.000 claims abstract description 10
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000195940 Bryophyta Species 0.000 claims abstract description 9
- FIPWRIJSWJWJAI-UHFFFAOYSA-N Butyl carbitol 6-propylpiperonyl ether Chemical compound C1=C(CCC)C(COCCOCCOCCCC)=CC2=C1OCO2 FIPWRIJSWJWJAI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000005696 Diammonium phosphate Substances 0.000 claims abstract description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 9
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- 239000004202 carbamide Substances 0.000 claims abstract description 9
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims abstract description 9
- 235000019838 diammonium phosphate Nutrition 0.000 claims abstract description 9
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- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 9
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- 229960005235 piperonyl butoxide Drugs 0.000 claims abstract description 9
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 9
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- 239000000463 material Substances 0.000 claims description 24
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 9
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- 235000019319 peptone Nutrition 0.000 claims description 9
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
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- 239000000284 extract Substances 0.000 claims description 6
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- 229920001592 potato starch Polymers 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000003895 organic fertilizer Substances 0.000 claims description 5
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
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- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
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- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of complex microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof, wherein prevent and treat the complex microorganism special fertilizer of celery soft rot, comprise the composition of following weight ratio: bacillus amyloliquefaciens 0.8-1 part, Bacillus thuringiensis 0.6-0.8 part, subtilis 0.5-0.7 part, milk-acid bacteria 0.4-0.8 part, substrate fermentation agent 1-3 part, maize straw 30-35 part, peat composed of rotten mosses 20-30 part, flyash 30-36 part, mushroom slag 10-20 part, expanded pig manure 30-40 part, methyl Synergistic Phosphorus pulvis 2-4 part, Piperonyl Butoxide pulvis 1-3 part, ethrel 0.4-0.6 part, urea 12-16 part, diammonium phosphate 10-12 part, potassium sulfate 6-8 part.The complex microorganism special fertilizer of celery soft rot that prepared by the present invention prevent and treat can make the soft rot sickness rate of celery greatly reduce, plant strain growth is more vigorous, and the output of celery is greatly enhanced, and quality also improves, probiotic bacterium in soil also increases, and the situation of soil is greatly improved.
Description
Technical field
The present invention relates to a kind of composite microbiological fertilizer, be specifically related to a kind of complex microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof, belong to technical field of fertilizers.
Background technology
Celery is high fiber food, and it produces the material of a kind of xylogen or intestines lactones through the digested effect of intestines, and this kind of material is a kind of antioxidant, often eat celery, especially leaf of Herba Apii graveolentis is eaten, all very useful to preventing hypertension, arteriosclerosis etc., and have auxiliary therapeutic action.Containing a large amount of compositions such as fiber, potassium, Lin Suanna Vitamin B2 Sodium Phosphate, vitamin PP in celery, these compositions can help us to relax bowel by human body, and regulate sodium-potassium balance, celery has a very important role to people in a word.
Celery soft rot, also known as " rotten pimple ", belongs to bacterial disease, mainly betides on petiole base or stem.
This disease mostly occurs the early growth period after celery transplants seedling-slowing stage or slow seedling.General elder generation falls ill from the petiole base of tender succulence.Their early stage, scab is filbert, water stain shape, fusiform or irregular shape, slightly cave in, after rapid expansion, interior tissue is that chocolate rots, foul smelling, finally only remains epidermis, whole celery lodging when falling ill serious, have a strong impact on the Quality and yield of celery, it is very important therefore to prevent and treat celery soft rot.
Feces of livestock and poultry in addition after crop material on-site incineration, the large-scale cultivation such as paddy rice, wheat, corn, rape such as to throw aside everywhere at the not only severe contamination environment, and also greatly wasting can as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrients resources (C, N, P, K, S and trace element) runs off outside soil-plant system, weakens the ability of China's agricultural sustainable development significantly.Therefore make full use of these resources also tool be of great significance.
At present mainly agricultural chemicals is utilized to the control of celery disease, this method employs a large amount of agricultural bactericides, a part is by celery bulk absorption, can damage human body, and vegetable grower is general in fertilising process adopts conventional chemical fertilizer, life-time service also has plant all to have a certain impact to soil, if so the solid organic castoff such as stalk, ight soil synthesizes high-grade organic compost through thermophilic fermentation, be used as the carrier of function yeast again, made microorganism organic fertilizer definite functions, will have good application prospect.At present the complex microorganism special fertilizer earned for preventing and treating celery soft rot be have not been reported.
Summary of the invention
The present invention is directed to present situation, a kind of complex microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof is provided, effectively can prevent the generation of celery soft rot, and improve its yield and quality, can also edaphic condition be improved.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
Prevent and treat a complex microorganism special fertilizer for celery soft rot, comprise the composition of following weight ratio: bacillus amyloliquefaciens 0.8-1 part, Bacillus thuringiensis 0.6-0.8 part, subtilis 0.5-0.7 part, milk-acid bacteria 0.4-0.8 part, substrate fermentation agent 1-3 part, maize straw 30-35 part, peat composed of rotten mosses 20-30 part, flyash 30-36 part, mushroom slag 10-20 part, expanded pig manure 30-40 part, methyl Synergistic Phosphorus pulvis 2-4 part, Piperonyl Butoxide pulvis 1-3 part, ethrel 0.4-0.6 part, urea 12-16 part, diammonium phosphate 10-12 part, potassium sulfate 6-8 part.
The above-mentioned preparation method preventing and treating the complex microorganism special fertilizer of celery soft rot, comprises the following steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on substratum, controls condition, finally respective bacteria containing amount or gemma amount>=6 × 10 of respective fermentation well
9individual/mL, makes bacterium liquid;
(2) bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and pig manure are carried out solid fermentation, turning every day 1 time in solid fermentation process, solid fermentation temperature is made to be no more than 50 DEG C, ferment through 6-8 days, bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, milk-acid bacteria continuation is simultaneously expanded numerous, obtain bacillus amyloliquefaciens microbial inoculum, Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculum reaches 5 × 10
9individual/more than g;
(3) substrate fermentation agent is joined according to described ratio in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, solid fermentation temperature is no more than 60 DEG C, when discovery material loosens, there is no the stink that material is original, and slightly ammonia taste, ferment when producing white hypha in heap complete, generally obtained through 8-10 days the fermented product that moisture content is 35-40%, it is numerous that the microorganism simultaneously in substrate fermentation agent obtains expansion;
(4) material that step (2) and step (3) obtain is mixed, and after-ripening 2-3 days, in After-ripening, turning every day is once, and turning 2-4 time, is evaporated to less than 30% by the water content of microbial organic fertilizer be finally no more than the condition of 60 DEG C in temperature under altogether;
(5) urea, diammonium phosphate, potassium sulfate, methyl Synergistic Phosphorus pulvis, Piperonyl Butoxide pulvis, ethrel are mixed according to described ratio, add water dissolution; And then the material obtained with step (4) mixes, and stirs;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 90 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 80 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
The fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2pO
4.Fermentation condition is: the fermentation flask of 60mL, charging capacity 60%, and temperature is 35-38 DEG C, ventilating ratio 1:1.3, and shaking speed is 7-7.2 at 180r/min, PH, fermentation period 24-26 hour.
The fermention medium of described solution Bacillus thuringiensis is LB liquid nutrient medium, and its proportioning is peptone 0.6g, yeast extract 0.27g, sodium-chlor 0.6g, and add water 50mL.Fermentation condition is: the fermentation flask of 60mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.4, and shaking speed is 7.5-8.5 at 220r/min, PH, fermentation period 28-30 hour.
The fermention medium Crossbred Cattle meat extract nutrient solution of described subtilis, its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, and potato starch 4g, is settled to 800mL, and pH value is adjusted to 7.2-7.4,121 DEG C of sterilizing 20min.Fermentation condition is: the fermentation flask of 1000mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.3, and shaking speed is 7.2-7.4 at 240r/min, PH, fermentation period 22-24 hour.
The fermention medium of described milk-acid bacteria is MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast leaching powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% magnesium sulfate, 0.02% manganous sulfate, 2% agar.Culture condition is: the fermentation flask of 500mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.2, and shaking speed is 6.2-6.4 at 240r/min, PH, fermentation period 26-28 hour.
Described bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains lead to growth Science and Technology Ltd. by Shanghai and produce; Substrate fermentation agent is produced by Beijing Zheng Nong agricultural science and technology company limited;
The invention has the beneficial effects as follows:
(1) soft rot of bacillus amyloliquefaciens to celery has certain antagonistic action, effectively can suppress the generation of soft rot.Can form advantage group after the microorganism species such as the Bacillus thuringiensis contained in fertilizer, subtilis, milk-acid bacteria are manured into soil, and continuation of surviving in soil is expanded numerous.
(2) the substrate fermentation agent selected by includes the ten multiple beneficial microorganisms such as fungi, yeast, actinomycetes and bacterium, adopt modern advanced biotechnology make each flora of its microorganism can harmonious coexistence, mutually promote, reach the effect of mutual supplement with each other's advantages.And contained probiotics Fast-propagation produce macrometabolic element in substrate fermentation process, can play and prevent and treat soil-borne disease, promotes the effect of plant growth.
(3) test-results shows, after the soil of plantation celery uses this product, significantly can fall the sickness rate of soft rot, biological control rate reaches more than 75%.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
embodiment 1
Prevent and treat a complex microorganism special fertilizer for celery soft rot, comprise the composition of following weight ratio: bacillus amyloliquefaciens 0.8 part, Bacillus thuringiensis 0.6 part, subtilis 0.5 part, milk-acid bacteria 0.4 part, substrate fermentation agent 1 part, maize straw 30 parts, the peat composed of rotten mosses 20 parts, 30 parts, flyash, mushroom slag 10 parts, expanded pig manure 30 parts, 2 parts, methyl Synergistic Phosphorus pulvis, 1 part, Piperonyl Butoxide pulvis, ethrel 0.4 part, 12 parts, urea, diammonium phosphate 10 parts, potassium sulfate 6 parts.
The above-mentioned preparation method preventing and treating the complex microorganism special fertilizer of celery soft rot, comprises the following steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on substratum, controls condition, finally respective bacteria containing amount or gemma amount>=6 × 10 of respective fermentation well
9individual/mL, makes bacterium liquid;
(2) bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and pig manure are carried out solid fermentation, turning every day 1 time in solid fermentation process, solid fermentation temperature is made to be no more than 50 DEG C, ferment through 6-8 days, bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, milk-acid bacteria continuation is simultaneously expanded numerous, obtain bacillus amyloliquefaciens microbial inoculum, Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculum reaches 5 × 10
9individual/more than g;
(3) substrate fermentation agent is joined according to described ratio in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, solid fermentation temperature is no more than 60 DEG C, when discovery material loosens, there is no the stink that material is original, and slightly ammonia taste, ferment when producing white hypha in heap complete, generally obtained through 8-10 days the fermented product that moisture content is 35-40%, it is numerous that the microorganism simultaneously in substrate fermentation agent obtains expansion;
(4) material that step (2) and step (3) obtain is mixed, and after-ripening 2-3 days, in After-ripening, turning every day is once, and turning 2-4 time, is evaporated to less than 30% by the water content of microbial organic fertilizer be finally no more than the condition of 60 DEG C in temperature under altogether;
(5) urea, diammonium phosphate, potassium sulfate, methyl Synergistic Phosphorus pulvis, Piperonyl Butoxide pulvis, ethrel are mixed according to described ratio, add water dissolution; And then the material obtained with step (4) mixes, and stirs;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 90 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 80 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
The fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2pO
4.Fermentation condition is: the fermentation flask of 60mL, charging capacity 60%, and temperature is 35-38 DEG C, ventilating ratio 1:1.3, and shaking speed is 7-7.2 at 180r/min, PH, fermentation period 24-26 hour.
The fermention medium of described solution Bacillus thuringiensis is LB liquid nutrient medium, and its proportioning is peptone 0.6g, yeast extract 0.27g, sodium-chlor 0.6g, and add water 50mL.Fermentation condition is: the fermentation flask of 60mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.4, and shaking speed is 7.5-8.5 at 220r/min, PH, fermentation period 28-30 hour.
The fermention medium Crossbred Cattle meat extract nutrient solution of described subtilis, its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, and potato starch 4g, is settled to 800mL, and pH value is adjusted to 7.2-7.4,121 DEG C of sterilizing 20min.Fermentation condition is: the fermentation flask of 1000mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.3, and shaking speed is 7.2-7.4 at 240r/min, PH, fermentation period 22-24 hour.
The fermention medium of described milk-acid bacteria is MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast leaching powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% magnesium sulfate, 0.02% manganous sulfate, 2% agar.Culture condition is: the fermentation flask of 500mL, and temperature is 30-32 DEG C, ventilating ratio 1:1.2, and shaking speed is 6.2-6.4 at 240r/min, PH, fermentation period 26-28 hour.
Described substrate fermentation agent is by Beijing Zheng Nong agricultural science and technology company limited specialty development & production, living bacteria count >=200,000,000/g, is specifically designed to and processes by the composite organic substrate of the various waste materials such as feces of livestock and poultry, agricultural crop straw, slag, river sand, flyash, mushroom slag.
embodiment 2
Prevent and treat a complex microorganism special fertilizer for celery soft rot, comprise the composition of following weight ratio: bacillus amyloliquefaciens 0.9 part, Bacillus thuringiensis 0.7 part, subtilis 0.6 part, milk-acid bacteria 0.6 part, substrate fermentation agent 2 parts, maize straw 32 parts, the peat composed of rotten mosses 25 parts, 33 parts, flyash, mushroom slag 15 parts, expanded pig manure 35 parts, 3 parts, methyl Synergistic Phosphorus pulvis, 2 parts, Piperonyl Butoxide pulvis, ethrel 0.5 part, 14 parts, urea, diammonium phosphate 11 parts, potassium sulfate 7 parts.
The fermentation proliferated culture medium of preparation method and various bacterium and culture condition thereof are with embodiment 1.
embodiment 3
Prevent and treat a complex microorganism special fertilizer for celery soft rot, comprise the composition of following weight ratio: bacillus amyloliquefaciens 1 part, Bacillus thuringiensis 0.8 part, subtilis 0.7 part, milk-acid bacteria 0.8 part, substrate fermentation agent 3 parts, maize straw 35 parts, the peat composed of rotten mosses 30 parts, 36 parts, flyash, mushroom slag 20 parts, expanded pig manure 40 parts, 4 parts, methyl Synergistic Phosphorus pulvis, 3 parts, Piperonyl Butoxide pulvis, ethrel 0.6 part, 16 parts, urea, diammonium phosphate 12 parts, potassium sulfate 8 parts.
The fermentation proliferated culture medium of preparation method and various bacterium and culture condition thereof are with embodiment 1.
In Ma Jiagou celery planting base, Qingdao, two process are set, each process three repetition, each community 80m
2, celery is unified plantation, outdoor cropping, unified management; Compound manure (the contrast 1 of complex microorganism special fertilizer of the present invention and the production of Qufu City Jin Shili science and technology Fei Ye company limited is used respectively with 50kg/ mu usage quantity, its total nutrient>=45%, N-P-K is 28-6-6), in vegetative period, field investigation is carried out to it, the situations such as the growth of investigation celery and soft rot generation, and survey its output after results, comparative result is in table 1:
Composite microbiological fertilizer prepared by table 1 the present invention and contrast 1 are compared
Result shows, the complex microorganism special fertilizer of celery soft rot that prepared by the present invention prevent and treat can make the soft rot sickness rate of celery greatly reduce, plant strain growth is more vigorous, the output of celery is greatly enhanced, quality also improves, probiotic bacterium in soil also increases, and the situation of soil is greatly improved.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (5)
1. prevent and treat the complex microorganism special fertilizer of celery soft rot for one kind, it is characterized in that, comprise the composition of following weight ratio: bacillus amyloliquefaciens 0.8-1 part, Bacillus thuringiensis 0.6-0.8 part, subtilis 0.5-0.7 part, milk-acid bacteria 0.4-0.8 part, substrate fermentation agent 1-3 part, maize straw 30-35 part, peat composed of rotten mosses 20-30 part, flyash 30-36 part, mushroom slag 10-20 part, expanded pig manure 30-40 part, methyl Synergistic Phosphorus pulvis 2-4 part, Piperonyl Butoxide pulvis 1-3 part, ethrel 0.4-0.6 part, urea 12-16 part, diammonium phosphate 10-12 part, potassium sulfate 6-8 part; Described a kind of preparation method preventing and treating the complex microorganism special fertilizer of celery soft rot, comprises the following steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on substratum, controls condition, finally respective bacteria containing amount or gemma amount>=6 × 10 of respective fermentation well
9individual/mL, makes bacterium liquid;
(2) bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and expanded pig manure are carried out solid fermentation, turning every day 1 time in solid fermentation process, solid fermentation temperature is made to be no more than 50 DEG C, ferment through 6-8 days, bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, milk-acid bacteria continuation is simultaneously expanded numerous, obtain bacillus amyloliquefaciens microbial inoculum, Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculum reaches 5 × 10
9individual/more than g;
(3) substrate fermentation agent is joined according to described ratio in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, solid fermentation temperature is no more than 60 DEG C, when discovery material loosens, there is no the stink that material is original, and slightly ammonia taste, ferment when producing white hypha in heap complete, generally obtained through 8-10 days the fermented product that moisture content is 35-40%, it is numerous that the microorganism simultaneously in substrate fermentation agent obtains expansion;
(4) material that step (2) and step (3) obtain is mixed, and after-ripening 2-3 days, in After-ripening, turning every day is once, and turning 2-4 time, is evaporated to less than 30% by the water content of microbial organic fertilizer be finally no more than the condition of 60 DEG C in temperature under altogether;
(5) urea, diammonium phosphate, potassium sulfate, methyl Synergistic Phosphorus pulvis, Piperonyl Butoxide pulvis, ethrel are mixed according to described ratio, add water dissolution; And then the material obtained with step (4) mixes, and stirs;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 90 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 80 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product; In step (1), the fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2pO
4.
2. the complex microorganism special fertilizer preventing and treating celery soft rot according to claim 1, it is characterized in that, the fermentation condition of described bacillus amyloliquefaciens is: the fermentation flask of 60mL, charging capacity 60%, temperature is 35-38 DEG C, ventilating ratio 1:1.3, and shaking speed is at 180r/min, pH is 7-7.2, fermentation period 24-26 hour; In step (1), the fermention medium of described Bacillus thuringiensis is LB liquid nutrient medium, and its proportioning is peptone 0.6g, yeast extract 0.27g, sodium-chlor 0.6g, and add water 50mL.
3. the complex microorganism special fertilizer preventing and treating celery soft rot according to claim 1, it is characterized in that, the fermentation condition of described Bacillus thuringiensis is: the fermentation flask of 60mL, temperature is 30-32 DEG C, ventilating ratio 1:1.4, shaking speed is 7.5-8.5 at 220r/min, pH, fermentation period 28-30 hour; In step (1), the fermention medium of described subtilis is Crossbred Cattle meat extract nutrient solution, and its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, and potato starch 4g, is settled to 800mL, and pH value is adjusted to 7.2-7.4,121 DEG C of sterilizing 20min.
4. the complex microorganism special fertilizer preventing and treating celery soft rot according to claim 1, it is characterized in that, the fermentation condition of described subtilis is: the fermentation flask of 1000mL, temperature is 30-32 DEG C, ventilating ratio 1:1.3, shaking speed is 7.2-7.4 at 240r/min, pH, fermentation period 22-24 hour; In step (1), the fermention medium of described milk-acid bacteria is MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast leaching powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% magnesium sulfate, 0.02% manganous sulfate, 2% agar.
5. the complex microorganism special fertilizer preventing and treating celery soft rot according to claim 1, it is characterized in that, the culture condition of described milk-acid bacteria is: the fermentation flask of 500mL, temperature is 30-32 DEG C, ventilating ratio 1:1.2, shaking speed is 6.2-6.4 at 240r/min, pH, fermentation period 26-28 hour.
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| CN104291948A (en) * | 2014-09-24 | 2015-01-21 | 林公传 | Celery planting controlled-release fertilizer and preparation method thereof |
| CN106146141A (en) * | 2016-06-24 | 2016-11-23 | 广德明凯蔬果种植家庭农场 | A kind of Herba Apii graveolentis special fertilizer |
| CN106748152A (en) * | 2016-12-23 | 2017-05-31 | 新昌县奥而特农业科技有限公司 | Composite microbiological fertilizer and preparation method thereof |
| CN106804406A (en) * | 2016-12-28 | 2017-06-09 | 安徽金禾肥业有限公司 | A kind of small sections of a vegetable plot organic substrate for preventing and treating cauliflower insect and preparation method thereof |
| CN107182515A (en) * | 2017-06-20 | 2017-09-22 | 合肥卓畅农业科技有限公司 | A kind of cultural method of celery |
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| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN101928186A (en) * | 2010-03-02 | 2010-12-29 | 万光存 | Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof |
| CN102126900A (en) * | 2010-01-20 | 2011-07-20 | 昆明榕风生物技术有限公司 | Controlled-release BB (Bulk Blending) fertilizer special for celery and production method thereof |
| CN102399106A (en) * | 2011-11-24 | 2012-04-04 | 吴昊宝 | Formula of compound fertilizer special for celery |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN102126900A (en) * | 2010-01-20 | 2011-07-20 | 昆明榕风生物技术有限公司 | Controlled-release BB (Bulk Blending) fertilizer special for celery and production method thereof |
| CN101928186A (en) * | 2010-03-02 | 2010-12-29 | 万光存 | Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof |
| CN102399106A (en) * | 2011-11-24 | 2012-04-04 | 吴昊宝 | Formula of compound fertilizer special for celery |
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