CN103267733A - Novel method for detecting scented tea antioxidant activity - Google Patents
Novel method for detecting scented tea antioxidant activity Download PDFInfo
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- CN103267733A CN103267733A CN2013100611640A CN201310061164A CN103267733A CN 103267733 A CN103267733 A CN 103267733A CN 2013100611640 A CN2013100611640 A CN 2013100611640A CN 201310061164 A CN201310061164 A CN 201310061164A CN 103267733 A CN103267733 A CN 103267733A
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Abstract
The present invention discloses a novel method for detecting scented tea antioxidant activity by using spectral changes during the growth of gold nanoparticles and achieving quantitative detection. Gold nanoparticles grow in gallic acid standard solutions of different concentrations. A spectrophotometer is used for measuring absorbance at a fixed wavelength. The relationship between the absorbance and the corresponding concentrations of antioxidants is drawn. In scented tea sample determination, the gallic acid solution is replaced by a scented tea extract, absorbance value is measured by a spectrophotometer at a specific wavelength, corresponding gallic acid equivalent in the sample is calculated by a standard curve, and the antioxidant activity is obtained. The invention is based on the change of spectral nature in the process of gold nanoparticle growth. Both the horizontal comparison for actual sample antioxidant activity and quantitative detection of sample antioxidant activity can be performed. The operation is easy and the cost is low.
Description
Technical field
The invention belongs to the nano biological analysis technical field, particularly a kind of method that detects polyphenoils total amount in the jasmine tea based on the growth of gold nano grain.
Background technology
The fusion of nanometer technology and biotechnology makes nanometer biotechnology obtain a lot of important progress.The application of nanometer technology has related to more and more many fields, biotechnology, and food, the nanometer detection technology has become the research focus.Gold nano grain is as research a kind of nano material early, and the application in biological detection causes common people's attention gradually.Gold nano grain has special physicochemical character, is significant for the sensitivity and the stability that improve biological detection.In recent years, the application of gold nano grain aspect food inspection also caused people's attention.
Chemical analysis (CA), thin layer chromatography (TLC), gas chromatography (GC), high performance liquid chromatography (HPLC) are generally adopted in food inspection analysis at present, but need pre-treatment loaded down with trivial details, consuming time, and the sample consume is bigger.Compare to the lower CA of sensitivity and TLC method, the sensitivity of GC, HPLC is higher relatively, but the operative technique requirement is high, instrument and equipment is expensive, and be not suitable for fast-field evaluation and popularize, and be the shortcoming that the detection technique of immune marker has only remedied these technology with the gold nano-material, cater to the modern requirement that detects, the utilization in the modern food analyzing and testing is also more and more.
The method of existing mensuration antioxidation activity has FRAP method, dimethyl sulfoxide method, salicylic acid method, TRAP method, DPPH method, ORAC method, methylene blue spectrophotometric method.These detection methods have not holding any government official post's advantage but also exist deficiency, on the basis of these methods, adopt nanometer technology to detect the inoxidizability of jasmine tea, can carry out quantitative test to the total amount of polyphenoils contained in the jasmine tea sample.
So far, Shang Weiyou uses nano material to detect the technology of polyphenoils total amount in jasmine tea inoxidizability and the quantitative sample.
Summary of the invention
1, goal of the invention
The object of the invention foot provides a kind of growth based on gold nano grain easy and simple to handle and that detection sensitivity is high to detect the inoxidizability power of jasmine tea and realize the polyphenoils quantitative methods.
2, technical scheme
The method of mensuration jasmine tea oxidation resistance power of the present invention, the variation of spectrum is as the standard reference frame when namely utilizing gold nano grain to grow in the gallic acid of variable concentrations, according to the variation of spectrum in the growth course of gold nano grain in the jasmine tea extract, estimate and measure the oxidation resistance of jasmine tea.
The method of mensuration jasmine tea oxidation resistance of the present invention, that is:
At K
2CO
3-HAuCl
4Under the mixed solution condition, the growth of gallic acid mediation gold nano grain.Gold nano grain is grown in the presence of the antioxidant of variable concentrations.
The power of antioxidant oxidation resistance is calculated in utilization in the variation of the spectral quality of the gold nano grain of the gallic acid growth from solution of variable concentrations.The drawing standard curve.
With the growth that the jasmine tea extract is replaced gallic acid solution mediation gold nano grain, the oxidation resistance of observing the change calculations jasmine tea sample of its spectral quality, be converted into the gallic acid equivalant of the contained polyphenoils of jasmine tea.
The present invention is concrete to adopt following technical scheme to solve the problem that proposes:
A kind of antioxidant based on gold nano grain is to the assay method of the oxidation resistance of jasmine tea, and step is:
1.K
2CO
3-HAuCl
4The preparation of mixed solution: with the K of 100mg
2CO
3Be dissolved in the 200mL pure water, stir 10min, add 1% the HAuCl of 3ml again
4In reaction solution, continue to stir 30min, solution is by the faint yellow water white transparency that slowly becomes.Place 4 ℃ of refrigerators to preserve this solution.
2. the preparation of gold seeds (AuNPs): in 200mL4 ℃ precooling pure water, add 3mL1%HAuCl
4, stir, add 1mL K again
2CO
3(0.2molL-1), the last 9mL NaBH that adds fast
4(0.5mgmL-1), stir 5min.Place 4 ℃ of preservations standby the AuNPs solution of the claret that obtains.
3. get 6mL K
2CO
3-HAuCl
4Mixed solution places on the magnetic stirring apparatus in reaction bulb, and average rate stirs, and adds the AuNPs of 100 μ L, adds 600 μ L ultrapure waters rapidly, stirs after 10 minutes, detects position and the absorption intensity of its ultraviolet absorption peak, and record.Above as blank.Repeat above-mentioned steps, 600 μ L ultrapure waters are become the gallic acid solution that concentration is 0.2mg/mL, 0.1mg/mL, 0.08mg/mL, 0.06mg/mL, 0.04mg/mL, 0.02mg/mL, 0.008mg/mL, 0.006mg/mL, 0.004mg/mL respectively.And drawing standard curve.
4. during the jasmine tea sample detection, repeating step 3,600 μ L gallic acid solution are replaced as the sample extraction liquid of equivalent, numerical value after the detection is brought the typical curve linear relationship of step 3 gained into, draw the concentration of the contained polyphenoils of jasmine tea, calculate the gallic acid equivalant of polyphenoils in this sample by formula.
Compared with prior art, the present invention has following advantage:
1. the present invention can finish under the operation of ultraviolet-visible spectrophotometer, and is easy and simple to handle, do not need complicated instrument and equipment.
2. do not relate to enzyme reaction in the experimental implementation process, experimental principle is clear and definite, does not also relate to poisonous and harmful carcinogen etc., can not produce any damaging effect for environment and human body.
3. experimental cost is low, and required reagent is the common reagent in laboratory, nano material easy to prepare, and single reaction only needs youngster minute minute can finish to ten youngsters, and the running time is short.
4. the present invention compares with the oxidation resistance that general spectrophotometric method detects sample, has visual detection prospect, and along with the concentration of gold grain and the variation of particle diameter, the reaction solution color changes gradually, and this method can be used in qualitative, the quantitative visual detection.
Description of drawings
Fig. 1: the digital photograph of the gold grain solution of variable concentrations gallic acid mediating growth
Fig. 2: the gold grain ultraviolet-visible spectrogram of variable concentrations gallic acid mediating growth
Fig. 3: gallic acid-gold grain absorbance linear relationship.
Embodiment
A kind of variation based on gold nano grain growth course spectrum is to the assay method of the oxidation resistance of jasmine tea, and step is:
1. dispose K
2CO
3-HAuCl
4The gallic acid solution of mixed solution, gold seeds, 0.004-0.2mg/mL.
2. get 6mL K
2CO
3-HAuCl
4Mixed solution and places on the magnetic stirring apparatus in reaction bulb, and average rate stirs, and adds the AuNPS of 100 μ L, adds 600 μ L gallic acid solution rapidly, stirs after 10 minutes, detects it in the absorption intensity at 521nm place, and record.Be horizontal ordinate with the gallic acid solution concentration, absorbance is ordinate, the drawing standard curve.
3. the mensuration of rose tea oxidation resistance.Take by weighing 1g rose tea tea powder and place the ultrapure water that 25ml boils and continue boiling and handled 10 minutes, after boiling is handled, with tea liquid vacuum filtration.
4. get 6mL K
2CO
3-HAuCl
4Mixed solution and places on the magnetic stirring apparatus in reaction bulb, and average rate stirs, and adds the AuNPS of 100 μ L, adds the rose sample extraction liquid after the 600 μ L dilution rapidly, stirs after 20 minutes, detects it in the absorption intensity at 521nm place.Bring the linear relationship equation that step 2 obtains into, obtain corresponding gallic acid concentration, calculate polyphenoils displacement gallic acid equivalant in the sample, be the amount of the polyphenoils that contains in the rose tea.
Embodiment 2
1. with embodiment 1 step 1.
2. with embodiment 1 step 2.
3. the mensuration of lemon tablet oxidation resistance.Step is with embodiment 1 step 3.
4. with embodiment 1 step 4, sample extraction stoste detects, and recording absorbance is 0.542, bring the linear relationship equation that step 2 obtains into, obtain corresponding gallic acid concentration, calculate polyphenoils displacement gallic acid equivalant in the sample, be the amount of the polyphenoils that contains in the lemon tablet.
Embodiment 3
1. with embodiment 1 step 1.
2. with embodiment 1 step 2.
3. the mensuration of green bamboo snake oxidation resistance.Step is with embodiment 1 step 3.
4. with embodiment 1 step 4,10 times of sample extraction liquid dilutions, recording absorbance is 0.637, bring the linear relationship equation that step 2 obtains into, obtain corresponding gallic acid concentration, calculate polyphenoils displacement gallic acid equivalant in the sample, be the amount of the polyphenoils that contains in the green bamboo snake.
Claims (8)
1. the present invention is a kind of method based on spectrum change detection jasmine tea oxidation resistance in the gold nano grain growth course, it is characterized in that carrying out according to the following steps:
1) K
2CO
3-HAuCl
4The preparation of mixed solution: with the K of 100mg
2CO
3Be dissolved in the 200mL pure water, stir 10min, add 1% the HAuCl of 3ml again
4In reaction solution, continue to stir 30min, solution is by the faint yellow water white transparency that slowly becomes.Place 4 ℃ of refrigerators to preserve this solution.
2) preparation of gold seeds (AuNPs): in 200mL4 ℃ precooling pure water, add 3mL1%HAuCl
4, stir, add 1mLK again
2CO
3(0.2molL
-1), the last 9mL NaBH that adds fast
4(O.5mgmL
-1), stir 5min, place 4 ℃ of preservations standby the AuNPs solution of the claret that obtains.
3) get 6ml K
2CO
3-HAuCl
4Mixed solution is in reaction bulb, place on the magnetic stirring apparatus, average rate stirs, the AuNPs that adds 100 μ L, add 600 μ L ultrapure waters rapidly, stir after 10 minutes, detect the absorption intensity of its absorption peak, and record, above as blank, repeat above-mentioned steps, with 600 μ L ultrapure waters become respectively concentration be 0.2mg/ml, O.1mg/ml, the gallic acid solution of 0.08mg/ml, 0.06mg/ml, 0.04mg/ml, 0.02mg/ml, 0.008mg/ml, 0.006mg/ml, 0.004mg/ml, and drawing standard curve.
4) during the jasmine tea sample detection, repeating step 3,600 μ L gallic acid solution are replaced as the sample extraction liquid of equivalent, numerical value after the detection is brought the typical curve linear relationship of step 3 gained into, draw the concentration of the contained polyphenoils of jasmine tea, calculate the gallic acid equivalant of polyphenoils in this sample, unit is milligram.
2. according to right 1, can use other inoxidizability material such as VC, VE to replace gallic acid.
3. according to right 1, jasmine tea all is dry back sample ultramicro grinding.
4. according to right 2, it is characterized in that antioxidation activity thing extraction solvent is pure water in the jasmine tea, tea powder and water ratio are 1: 25 when mentioning.
5. according to right 2, when active matter extracts in the jasmine tea under fluidized state 10min.
6. according to right 1, temperature of reaction is 25 ℃.
7. according to right 1, detect the method for jasmine tea inoxidizability based on the spectrum change of nanogold particle growth course, it is characterized in that the uv-visible absorption spectra peak scope of gold nano grain to be measured is 510～580nm.
8. according to right 1, it is characterized in that realizing the mensuration of the polyphenoils total amount in the jasmine tea.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644997A (en) * | 2017-03-13 | 2017-05-10 | 江南大学 | Detecting method for detecting fruit and vegetable extract oxidation resistant capability by using gold nanometer material optical absorption characteristic |
| CN107884400A (en) * | 2017-11-09 | 2018-04-06 | 无限极（中国）有限公司 | The quantitative detecting method of total polyphenols in a kind of Purple Perilla Seed Oil |
| CN108918437A (en) * | 2018-05-16 | 2018-11-30 | 天津中医药大学 | A kind of the antioxidant activity detection method and application of blood-activating and stasis-removing preparation |
| CN112255202A (en) * | 2020-10-16 | 2021-01-22 | 北京师范大学 | Method for measuring antioxidant capacity |
| CN113617395A (en) * | 2021-09-02 | 2021-11-09 | 河北农业大学 | Nano enzyme for detecting antioxidant activity of food and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101762559A (en) * | 2010-02-02 | 2010-06-30 | 河南农业大学 | Method for detecting antioxidant capacity of plant and plant-derived liquid food based on nanogold system |
| CN102721653A (en) * | 2012-07-11 | 2012-10-10 | 蒋彩云 | Method for detecting heavy metal lead based on nanogold chain material |
-
2013
- 2013-02-27 CN CN2013100611640A patent/CN103267733A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101762559A (en) * | 2010-02-02 | 2010-06-30 | 河南农业大学 | Method for detecting antioxidant capacity of plant and plant-derived liquid food based on nanogold system |
| CN102721653A (en) * | 2012-07-11 | 2012-10-10 | 蒋彩云 | Method for detecting heavy metal lead based on nanogold chain material |
Non-Patent Citations (1)
| Title |
|---|
| MATTEO SCAMPICCHIO ETC.: "Nanoparticle-Based Assays of Antioxidant Activity", 《ANALYTICAL CHEMISTRY》, vol. 78, no. 6, 10 February 2006 (2006-02-10) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644997A (en) * | 2017-03-13 | 2017-05-10 | 江南大学 | Detecting method for detecting fruit and vegetable extract oxidation resistant capability by using gold nanometer material optical absorption characteristic |
| CN107884400A (en) * | 2017-11-09 | 2018-04-06 | 无限极（中国）有限公司 | The quantitative detecting method of total polyphenols in a kind of Purple Perilla Seed Oil |
| CN108918437A (en) * | 2018-05-16 | 2018-11-30 | 天津中医药大学 | A kind of the antioxidant activity detection method and application of blood-activating and stasis-removing preparation |
| CN112255202A (en) * | 2020-10-16 | 2021-01-22 | 北京师范大学 | Method for measuring antioxidant capacity |
| CN113617395A (en) * | 2021-09-02 | 2021-11-09 | 河北农业大学 | Nano enzyme for detecting antioxidant activity of food and preparation method thereof |
| CN113617395B (en) * | 2021-09-02 | 2022-05-17 | 河北农业大学 | Nano enzyme for detecting antioxidant activity of food and preparation method thereof |
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Application publication date: 20130828 |