CN103267732A - Full-range imaging method and system based on mobile optical grating spatial carrier frequency spectral domain OCT (optical coherence tomography) - Google Patents
Full-range imaging method and system based on mobile optical grating spatial carrier frequency spectral domain OCT (optical coherence tomography) Download PDFInfo
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Abstract
The invention discloses a full-range imaging method and system based on mobile optical grating spatial carrier frequency spectral domain OCT (optical coherence tomography). According to the method and the system, a mobile optical grating replaces a reference mirror in the conventional spectral domain OCT, and a spatial carrier frequency which does not need optical path modulation is introduced in the transverse scanning direction. A detected interference spectrum signal is subjected to Fourier transform along the transverse scanning direction at first, then bandpass filtering centering on the amount of the spatial carrier frequency is carried out, then the filtered signal is subjected to inverse Fourier transform, and finally, the signal is subjected to Fourier transform along the axial direction to obtain full-range OCT images of a sample after mirror images are eliminated. By adopting the method and system, the full-range spectral domain OCT images can be obtained on the premise that any additional optical path difference is not introduced, and the sensitivity drop caused by accumulated optical path differences in the conventional spatial carrier frequency method is avoided.
Description
Technical field
The present invention relates to optical coherent chromatographic imaging (OCT) technology, relate in particular to a kind of based on mobile grating space carrier frequency spectral coverage OCT gamut formation method and system.
Background technology
Optical coherent chromatographic imaging (Optical Coherence Tomography OCT) is a kind of non-intruding, non-contacting imaging technique, as a kind of brand-new, develop imaging technique rapidly, have series of advantages such as high-resolution, harmless, real time imagery.And spectral coverage OCT (Spectral domain OCT, SD-OCT) system is as second generation OCT technology, and (Time domain OCT TD-OCT), has clear superiority at aspects such as image taking speed, signal to noise ratio (S/N ratio) and sensitivity than first generation OCT technology.The greatest problem of SD-OCT system existence at present is the mirror image that is caused by the complex conjugate item that the real function Fourier transform produces.In actual applications, for fear of obscuring of mirror image and real image, generally testing sample is placed on a side of zero optical path difference position, and near the highest zone of sensitivity often zero light path, therefore eliminate mirror image, imaging depth is doubled, realize the gamut imaging, always be the focus of SD-OCT research.
People have proposed the whole bag of tricks and have made up the interference spectrum signal of plural form to eliminate the conjugation item.The method that obtains multiple interference spectrum signal the earliest is based on phase shift interference art (Phase Shifting Interferometry, PSI) theory.M.Wojtkowski realizes the transposition of five steps based on the PSI method in the SD-OCT system, by amplitude and the phase information that calculates interference spectrum, thereby obtain multiple interference spectrum.And the most frequently used method of elimination complex conjugate mirror image is the linear B-M method that is proposed by Y. Yasuno in the present SD-OCT system.Linear B-M method can be thought the expansion of traditional transposition method, has data processing speed more efficiently, can suppress the noise that is caused by chromatic dispersion transposition error simultaneously.But in linear B-M method, introduced transposition amount pi/2 between the adjacent A-scan, if the transversal scanning scope will add up a bigger optical path difference more greatly.Owing to the sensitivity of the SD-OCT system increase along with imaging depth reduces, thus optical path difference of this accumulative total can cause last image transversely the direction of scanning signal to noise ratio (S/N ratio) descend.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, proposed a kind of based on mobile grating space carrier frequency spectral coverage OCT gamut formation method and system.
The objective of the invention is to be achieved through the following technical solutions:
A kind of spectral coverage OCT gamut formation method based on mobile grating space carrier frequency of the present invention:
In spectral coverage OCT, based on the method for linear BM-mode, utilize the phase compensating factor in the grating, the OCT image behind the mirror image that successfully obtained to disappear, and do not introduce group velocity delay, its concrete steps are as follows:
Step 1: in the spectral coverage OCT system, reference light is radiated on the grating with Littrow condition, and namely the incident angle of chief ray equals angle of diffraction in the reference light, and grating, decomposes speed v along the translation of grating face with speed v, obtains
With
Direction, when grating with speed
When being moved to the left, be equivalent to collimating mirror with speed
Move horizontally to the right, elapsed time t, collimating mirror 1 move to position 2 from the position.One section light path has been introduced with respect to position more than 1 in position 2
Consider the phase compensating factor of grating, position 2 is with respect to position 1, and phase place does not change, so light path
Only introduced group velocity delay, do not introduced phase velocity and postpone.Consider
Direction, speed
Produced the motion of vertical direction, same elapsed time t, collimating mirror 1 moves to position 2 from the position so.One section light path has been introduced with respect to position 1 in position 2
This section light path can be introduced group velocity delay and phase velocity simultaneously and postpone, but group velocity delay just with light path
Only the group velocity delay of introducing is cancelled out each other, so reference light only can be introduced one compared to sample light is last
The phase velocity that produces postpones
This reference light and sample light interfere at last, and interference signal is received by high speed linear array CCD.
Step 2: in the spectral coverage OCT system, feeler arm receives interference signal at last, earlier interference signal is carried out horizontal Fourier transform, owing to introduced the phase velocity delay, in this space, the spatial frequency spectrum of real signal and complex conjugate signal is separated, leach the real signal part by the filtering of windowing, carry out inverse Fourier transform then, just obtained complex signal, at last this complex signal is carried out Fourier transform vertically, the OCT image behind the mirror image that can obtain to disappear.
A kind of spectral coverage OCT gamut imaging system based on mobile grating space carrier frequency of the present invention mainly comprises wideband light source, 2x2 broadband optical fiber coupler, 1x2 broadband optical fiber coupler, sample arm, reference arm, feeler arm.Sample arm comprises first collimation lens, X-Y scanning galvanometer, first convergent lens, sample; Reference arm comprises second collimation lens, first grating, second convergent lens, the 3rd convergent lens, second grating, the 3rd collimation lens, stepper motor; Feeler arm comprises the 4th collimation lens, the 3rd grating, the 4th convergent lens, high speed linear array CCD.
Wideband light source links to each other with an input end of 2x2 broadband optical fiber coupler, an output terminal of 2x2 broadband optical fiber coupler links to each other with first collimation lens, the X-Y scanning galvanometer is positioned on the emitting light path of first collimation lens, first convergent lens is positioned on the reflected light path of X-Y scanning galvanometer, the X-Y scanning galvanometer is positioned at the front focal plane of first convergent lens, and sample is positioned at the back focal plane of first convergent lens; Another output terminal of 2x2 broadband optical fiber coupler links to each other with second collimation lens; First grating is fixed on the stepper motor, be arranged in the emitting light path of second collimation lens, second convergent lens is positioned on the first grating reflection light path, the 3rd convergent lens is positioned at the emitting light path of second convergent lens, the back focal plane of the front focal plane of the 3rd convergent lens and second convergent lens overlaps, second grating is positioned on the emitting light path of the 3rd convergent lens, and the 3rd collimation lens is positioned on the second grating reflection light path; Input end of 1x2 broadband optical fiber coupler and another input end of 2x2 broadband optical fiber coupler link to each other, another input end of 1x2 broadband optical fiber coupler links to each other with the 3rd collimation lens, and the output terminal of 1x2 broadband optical fiber coupler links to each other with the 4th collimation lens.The 3rd grating is positioned on the emitting light path of the 4th collimation lens, and the 4th convergent lens is positioned on the emitting light path of the 3rd grating, and high speed linear array CCD is positioned on the back focal plane of the 4th convergent lens.
Compare with background technology, the beneficial effect that the present invention has is:
1, than general OCT system, this method can replace the level crossing in the reference arm along the grating of grating face translation with one, can introduce the required phase velocity of the mirror image that disappears very easily postpones, handle the gamut OCT image that just can obtain not having mirror image by simple filtering, imaging depth is doubled;
2, than general linear B-M method, this method disappears when the required phase velocity of mirror image postpones in introducing, does not introduce any group velocity delay.In linear B-M method, introduced transposition amount pi/2 between the adjacent A-Scan, if the transversal scanning scope will add up a bigger optical path difference more greatly.Owing to the sensitivity of spectral coverage OCT (SD-OCT) the system increase along with imaging depth reduces, thus optical path difference of this accumulative total can cause last image transversely the direction of scanning signal to noise ratio (S/N ratio) descend.This method can not cause signal to noise ratio (S/N ratio) to descend because of the relation of sensitivity, so image quality can not descend when eliminating mirror image.
Description of drawings
Fig. 1 is spectral domain optical coherence tomography system synoptic diagram of the present invention;
Fig. 2 is the spatial frequency spectrum distribution plan in the data handling procedure described in the present invention;
Fig. 3 is reference arm part principle schematic among the present invention.
Among the figure: 1, wideband light source, 2, the 2x2 broadband optical fiber coupler, 3, collimation lens, 4, the X-Y scanning galvanometer, 5, convergent lens, 6, sample, 7, collimation lens, 8, grating, 9, convergent lens, 10, convergent lens, 11, grating, 12, collimation lens, 13,1x2 broadband optical fiber coupler, 14, collimation lens, 15, grating, 16, convergent lens, 17, high speed linear array CCD, 18, sample arm, 19, reference arm, 20, feeler arm, 21, stepper motor.
Specific embodiments
A kind of spectral coverage OCT gamut formation method based on mobile grating space carrier frequency of the present invention, this method may further comprise the steps:
Step 1: in the spectral coverage OCT system, reference light is radiated on the grating with Littrow condition, and namely the incident angle of chief ray equals angle of diffraction in the reference light, and grating, decomposes speed v along the translation of grating face with speed v, obtains
With
Direction, when grating with speed
When being moved to the left, be equivalent to collimating mirror with speed
Move horizontally to the right, elapsed time t, collimating mirror 1 move to position 2 from the position.One section light path has been introduced with respect to position more than 1 in position 2
Consider the phase compensating factor of grating, position 2 is with respect to position 1, and phase place does not change, so light path
Only introduced group velocity delay, do not introduced phase velocity and postpone.Consider
Direction, speed
Produced the motion of vertical direction, same elapsed time t, collimating mirror 1 moves to position 2 from the position so.One section light path has been introduced with respect to position 1 in position 2
This section light path can be introduced group velocity delay and phase velocity simultaneously and postpone, but group velocity delay just with light path
Only the group velocity delay of introducing is cancelled out each other, so reference light only can be introduced one compared to sample light is last
The phase velocity that produces postpones
This reference light and sample light interfere at last, and interference signal is received by high speed linear array CCD.
Step 2: in the spectral coverage OCT system, feeler arm receives interference signal at last, earlier interference signal is carried out horizontal Fourier transform, owing to introduced the phase velocity delay, in this space, the spatial frequency spectrum of real signal and complex conjugate signal is separated, leach the real signal part by the filtering of windowing, carry out inverse Fourier transform then, just obtained complex signal, at last this complex signal is carried out Fourier transform vertically, the OCT image behind the mirror image that can obtain to disappear.
As shown in Figure 1, the present invention mainly comprises wideband light source 1,2x2 broadband optical fiber coupler 2,1x2 broadband optical fiber coupler 13, sample arm 18, reference arm 19, feeler arm 20.Sample arm comprises first collimation lens 3, X-Y scanning galvanometer 4, the first convergent lenses 5, sample 6; Reference arm comprises second collimation lens, 7, the first gratings, 8, the second convergent lenses, 9, the three convergent lenses, 10, the second gratings, 11, the three collimation lenses 12, stepper motor 21; Feeler arm comprises the 4th collimation lens 14, the three gratings 15, the four convergent lenses 16, high speed linear array CCD 17.
Wideband light source 1 links to each other with an input end of 2x2 broadband optical fiber coupler 2, an output terminal of 2x2 broadband optical fiber coupler links to each other with first collimation lens 3, X-Y scanning galvanometer 4 is positioned on the emitting light path of first collimation lens 3, first convergent lens 5 is positioned on the reflected light path of X-Y scanning galvanometer 4, X-Y scanning galvanometer 4 is positioned at the front focal plane of first convergent lens 5, and sample 6 is positioned at the back focal plane of first convergent lens 5; Another output terminal of 2x2 broadband optical fiber coupler links to each other with second collimation lens 7; First grating 8 is fixed on the stepper motor 21, be arranged in the emitting light path of second collimation lens 7, second convergent lens 9 is positioned on the reflected light path of first grating 8, the 3rd convergent lens 10 is positioned at the emitting light path of second convergent lens 9, the back focal plane of the front focal plane of the 3rd convergent lens 10 and second convergent lens 9 overlaps, second grating 11 is positioned on the emitting light path of the 3rd convergent lens 10, and the 3rd collimation lens 12 is positioned on the second grating reflection light path; Input end of 1x2 broadband optical fiber coupler 13 and another input end of 2x2 broadband optical fiber coupler 2 link to each other, another input end of 1x2 broadband optical fiber coupler 13 links to each other with the 3rd collimation lens 12, and the output terminal of 1x2 broadband optical fiber coupler 13 links to each other with the 4th collimation lens 14.The 3rd grating 15 is positioned on the emitting light path of the 4th collimation lens 14, and the 4th convergent lens 16 is positioned on the emitting light path of the 3rd grating 15, and high speed linear array CCD 17 is positioned on the back focal plane of the 4th convergent lens 16.
The low-coherent light that comes out from wideband light source 1, incide 2x2 broadband optical fiber coupler 2, after light splitting, one the tunnel enters sample arm 18, through first collimation lens 3, be radiated on the sample 6 behind X-Y scanning galvanometer 4, the first convergent lenses 5, flashlight through sample 6 reflected backs returns along former road, through 2x2 broadband optical fiber coupler 2 to 1x2 broadband optical fiber couplers 13 places;
As shown in Figure 3, enter reference arm 19 from another Shu Guang of 2x2 broadband optical fiber coupler 2 outgoing, through second collimation lens 7, be radiated on first grating 8, first grating 8 is fixed on the stepper motor 21, stepper motor 21 along the translation of grating face, is introduced modulated carriers with certain speed v by computer control
, reference light is radiated on second grating 11 after 8 light splitting of first grating are by second convergent lens, 9, the three convergent lenses 10, and diffraction light is converged to directional light again again and is entered second collimation lens 12, then to 1x2 broadband optical fiber coupler 13 places;
Sample light and reference light meet at 1x2 broadband optical fiber coupler 13 places, and interfere, and interference light enters feeler arm 20, through being radiated on the 3rd grating 15 behind the 4th collimation lens 14.Behind grating beam splitting, the coloured light of different wave length focuses on diverse location on the high speed linear array CCD 17 through the 4th convergent lens 16, and 17 pairs of interference spectrums of high speed linear array CCD are gathered, and the spectral signal of collection imports computing machine at last into.In computing machine, to the advanced laterally Fourier transform of the interference spectrum that collects, obtain space distribution as shown in Figure 2, because introduce modulated carriers
Cause, the spatial frequency spectrum of complex conjugate item and real image is separated, leach the real image part on the right by the filtering of windowing, carry out inverse Fourier transform then, just obtained the interference spectrum signal of plural form, this signal is carried out Fourier transform more vertically, the sample structure pattern behind the mirror image that just obtained disappearing.
Claims (2)
1. spectral coverage OCT gamut formation method based on mobile grating space carrier frequency, this method specifically may further comprise the steps:
Step 1: in the spectral coverage OCT system, reference light is radiated on the grating with Littrow condition, and namely the incident angle of chief ray equals angle of diffraction in the reference light, and grating, decomposes speed v along the translation of grating face with speed v, obtains
With
Direction, when grating with speed
When being moved to the left, be equivalent to collimating mirror with speed
Move horizontally to the right, elapsed time t, collimating mirror 1 move to position 2 from the position; One section light path has been introduced with respect to position more than 1 in position 2
Consider the phase compensating factor of grating, position 2 is with respect to position 1, and phase place does not change, so light path
Only introduced group velocity delay, do not introduced phase velocity and postpone; Consider
Direction, speed
Produced the motion of vertical direction, same elapsed time t, collimating mirror 1 moves to position 2 from the position so; One section light path has been introduced with respect to position 1 in position 2
This section light path can be introduced group velocity delay and phase velocity simultaneously and postpone, but group velocity delay just with light path
Only the group velocity delay of introducing is cancelled out each other, so reference light only can be introduced one compared to sample light is last
The phase velocity that produces postpones
This reference light and sample light interfere at last, and interference signal is received by high speed linear array CCD;
Step 2: in the spectral coverage OCT system, feeler arm receives interference signal at last, earlier interference signal is carried out horizontal Fourier transform, owing to introduced the phase velocity delay, in this space, the spatial frequency spectrum of real signal and complex conjugate signal is separated, leach the real signal part by the filtering of windowing, carry out inverse Fourier transform then, just obtained complex signal, at last this complex signal is carried out Fourier transform vertically, the OCT image behind the mirror image that can obtain to disappear.
2. the spectral coverage OCT gamut imaging system based on mobile grating space carrier frequency mainly comprises wideband light source, 2x2 broadband optical fiber coupler, 1x2 broadband optical fiber coupler, sample arm, reference arm, feeler arm; Sample arm comprises first collimation lens, X-Y scanning galvanometer, first convergent lens, sample; Reference arm comprises second collimation lens, first grating, second convergent lens, the 3rd convergent lens, second grating, the 3rd collimation lens, stepper motor; Feeler arm comprises the 4th collimation lens, the 3rd grating, the 4th convergent lens, high speed linear array CCD;
It is characterized in that: wideband light source links to each other with an input end of 2x2 broadband optical fiber coupler, an output terminal of 2x2 broadband optical fiber coupler links to each other with first collimation lens, the X-Y scanning galvanometer is positioned on the emitting light path of first collimation lens, first convergent lens is positioned on the reflected light path of X-Y scanning galvanometer, the X-Y scanning galvanometer is positioned at the front focal plane of first convergent lens, and sample is positioned at the back focal plane of first convergent lens; Another output terminal of 2x2 broadband optical fiber coupler links to each other with second collimation lens; First grating is fixed on the stepper motor, be arranged in the emitting light path of second collimation lens, second convergent lens is positioned on the first grating reflection light path, the 3rd convergent lens is positioned at the emitting light path of second convergent lens, the back focal plane of the front focal plane of the 3rd convergent lens and second convergent lens overlaps, second grating is positioned on the emitting light path of the 3rd convergent lens, and the 3rd collimation lens is positioned on the second grating reflection light path; Input end of 1x2 broadband optical fiber coupler and another input end of 2x2 broadband optical fiber coupler link to each other, another input end of 1x2 broadband optical fiber coupler links to each other with the 3rd collimation lens, and the output terminal of 1x2 broadband optical fiber coupler links to each other with the 4th collimation lens; The 3rd grating is positioned on the emitting light path of the 4th collimation lens, and the 4th convergent lens is positioned on the emitting light path of the 3rd grating, and high speed linear array CCD is positioned on the back focal plane of the 4th convergent lens.
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| CN105559756A (en) * | 2016-02-05 | 2016-05-11 | 浙江大学 | Microangiography method and system based on total space modulation spectrum segmentation angle combining |
| CN105811236A (en) * | 2016-05-09 | 2016-07-27 | 中国科学院上海光学精密机械研究所 | Laser frequency tuning method for laser |
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| CN110017794A (en) * | 2019-04-10 | 2019-07-16 | 南京理工大学 | A kind of dynamic phasing deformation interferometric measuring means and method |
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| CN105559756A (en) * | 2016-02-05 | 2016-05-11 | 浙江大学 | Microangiography method and system based on total space modulation spectrum segmentation angle combining |
| CN105559756B (en) * | 2016-02-05 | 2019-11-15 | 浙江大学 | Based on the compound microangiography method and system of total space modulation spectrum segmentation angle |
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| CN105811236B (en) * | 2016-05-09 | 2019-02-26 | 中国科学院上海光学精密机械研究所 | The tuning methods of laser device laser frequency |
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