CN103238849A - Production method and application of sericin microcapsules - Google Patents
Production method and application of sericin microcapsules Download PDFInfo
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- CN103238849A CN103238849A CN 201210031770 CN201210031770A CN103238849A CN 103238849 A CN103238849 A CN 103238849A CN 201210031770 CN201210031770 CN 201210031770 CN 201210031770 A CN201210031770 A CN 201210031770A CN 103238849 A CN103238849 A CN 103238849A
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Abstract
The invention relates to a production method of sericin microcapsules, which can be used for preparing sericin into sericin cyclodextrine (beta-CD) microcapsules, sericin sodium alginate microcapsules and sericin sodium alginate polylysine microcapsules for processing foods and drinks. According to the invention, the sericin microcapsules are used for the markets of reprocessing of sericin in foods, health-care foods or medicines and particularly the markets of processing of sericin in drinks, and are suitable for being mixed with cyclodextrine-embedded extracts of multiple plant sources to prepare transparent drinks or bead-suspended drinks like pearl milk tea. The production method provided by the invention expands the new sources of food additives, develops new ways of sericin in application, promotes the resource utilization of waste sericin, and has greater potential markets.
Description
Technical field
The present invention relates to a kind of silk gum microcapsules production method and application, specifically, is the method with the silk gum microencapsulation, it is characterized in that silk gum is made the microencapsulation product that can be used for processed food beverage or pharmaceutical oral liquid.
Background technology
Silk gum is to be coated on the outer field colloid protein of silk silk quality.Micro-yellow powder, soluble in water, ninhydrin reaction is positive.Serine and asparatate isopolarity side group amino acid are high in the silk gum.Be the profuse protein of a kind of hydrophilic radical.Because silk gum unique biological compatibility has been widely used in industries such as biology, medical science, weaving.The extraction of silk gum can make a large amount of silk gum in the silk textile industry waste water turn waste into wealth, and for the processing of enterprise's sewage, the improvement of economic benefit and environment plays crucial effects.
After silk gum and fibroin mixed in certain proportion, by regulating pH silk gum and fibroin albumen are condensed again, the food of making thus is softness, good to eat extremely.Sericin still is the good binder of freezing cake, as freezing cake binder, can prevent its cake shape damage with sericin.Silk-fibroin comprises that the hydrolysate of silk gum and fibroin also is used for the baste that some melon and fruit food is pickled, and sericin has the exploitation future very much aspect food.The good fortune of hillside plot English has also been developed a kind of diet that adds silk gum or silk gum hydrolysate and has been tasted with discrimination water, and the food of this diet water, meal, tea, beverage etc. can be kept health, suppress germ, delay senility, beautifying face and moistering lotion.Existing this series products is sold [1] on the Japanese market at present.
In beverage or food, can promote the absorption to mineral matter behind the adding silk gum hydrolysate.Silk gum and silk gum hydrolysate thereof can be used as food additives and the health nutrient agent of indigestibility food, can eliminate the absorption of constipation, promotion mineral matter nutritional, help improvement, the enhancing human body constitution [1] of intestinal physiology function.
Recently. nonessential amino acid one serine that there are some researches show the secretion of myelencephalon astrocyte is brain neuron existence and grows necessary.L one serine accounts for serine total amount 33.3% in the sericin, and glycine also contains 16.1%, and this two seed amino acid accounts for half of total amount.Therefore, absorb that sericin or silk gum hydrolysate can improve man memory power, prevent brain aging more, prevention dementia etc. [1].
Japan finds that silk gum and hydrolysate thereof all have inhibitory action to colorectal cancer, cutaneum carcinoma.Edible contain that 3% silk gum feed reached 115 days and the male mouse of 1,2 one Dimethylhydrazine (DMH) of 10 all jede Woches injections originally, incidence and the quantity of its intestinal cancer are suppressed.These results show that silk gum and hydrolysate thereof have potential value as a kind of prophylaxis of colon cancer medicament.Experimental mouse with the coating of 5.0mg silk gum is not observed the symptom [1] that any cutaneum carcinoma takes place.
Silk gum can be accelerated alcohol effectively and directly get rid of by urine and the interior alcohol oxidation efficient of reinforcement liver; simultaneously; silk gum can play certain protection by removing active oxidation species and raising activities of antioxidant enzymes and be used for suppressing lipid peroxidation; can effectively suppress polyphenol oxidase activity in the food; be the fabulous natural of fatty foods, the additive that exploitation is prolonged the freshness date of milk food has the value [1] of highly significant.
Silk gum and cocoon shell water decoction have hypoglycemic activity: diabetes, the traditional Chinese medical science is referred to as diabetes.Chinese traditional treatment is quenched one's thirst it from ancient times, and silk cocoon, stiff silkworm or the mulberry leaf of silkworm commonly used (Bombyx mori).Jin Dynasty well-known doctor Zhang Congzheng says: " go up with filature cocoon cooking soup, clarification, that pauses and obey then heals ", the composition of cocoon cooking soup is exactly silk gum [2].Modern times experimental results show that the domestic silkworm silk rubber powder has effect more significantly to treatment of diabetes, and blood sugar decreasing effect is inferior to glibenclamide [3].Silk gum can effectively reduce diabetes B rat BG and rising has certain prevention effect [4] to diabetes BG.Silk gum can be by the imbalance of rise kidney pedf protein, kidney blood vessel generation when downward modulation kidney vegf protein improves diabetic nephropathy, and performance is to protection and the prevention effect [5] of diabetic nephropathy rat kidney.Damage has protective effect to silk gum to the type ii diabetes pancreas islet; And effect and melbine be [5] quite.Silk gum can obviously improve the expression of diabetes B rat spinal cord anterior angle neuron NGF, and performance is to the protective effect [7] of diabetic neuropathy.Cocoon shell water decoction does not have hypoglycemic activity to normal mouse, and the alloxan diabetes model mice is had hypoglycemic activity, and presents certain dose-effect and time-effect relationship, has the effect characteristics [8] of two arc compounds.The blood sugar decreasing effect of silk cocoon extract is better than diabetes pill [9].Silk cocoon does not have obvious influence to the blood sugar of normal mouse, and paraffin section shows that pancreas islet and the pancreas islet mouth cells degree of necrosis of high dose silk cocoon group diabetes rat pancreas obviously reduce.The mechanism that the silk cocoon hypoglycemic activity is described may promote the reparation of diabetes rat pancreatic damage due to the alloxan with silk cocoon, and promotes insulin secretion and reduction insulin decomposition rate relevant [10,11].
Above data shows, silk gum illustrates also have the market potential demand aspect corresponding health food, health drink, the oral drugs having showed function the more aspect the prevention of internal disease and the treatment.Japan's developing trend has also confirmed the potential prospect of silk gum food.Solve the microencapsulation of silk gum, silk gum just had with multiple food food combination particularly some drinks combination may, the present invention designs in order to adapt to this demand exactly.
Silk gum is kind of a polypeptide, the microencapsulation of polypeptides matter microencapsulation and non-protein medicaments and active enzyme microencapsulation, and the selection of its wall material and encystation technology all is not quite similar.The present invention selects cyclodextrine, and (β-CD) and sodium alginate are that main wall material is made silk gum microcapsules [12,13,14].
Summary of the invention
The present invention relates to a kind of silk gum microcapsules production method, specifically utilize known method to realize the silk gum microencapsulation, it is characterized in that silk gum is made the microencapsulation product that can be used for food and drink processing.
The so-called glue of the present invention, from silkworm, rather than from the silk gum of tussah or other essay kind silkworms,
The so-called high concentration silk gum of the present invention liquid, the major function composition is sericin.
This high concentration silk gum liquid can be formulated with pure silk glue finished product, or from the degumming waste water of reeling mill, spun silk factory, silk mill, silk quilt factory through removal of impurities, except not Edible material, degerming, appropriateness concentrate and get, perhaps weaving silk from the silkworm kind of only secreting silk gum forms through modulation.
The present invention is towards the reprocessing market of silk gum at food, health food or medicine, refers in particular to the processing of beverage drink, considers current market-orientedly, and emphasis of the present invention relates to the processing technology demand of similar tea beverage or Pearled tea beverage market.Therefore, silk gum microcapsules involved in the present invention, the main sensing is that (β-CD) and sodium alginate are made miniature silk gum microcapsules or small ball shape silk gum microcapsules as the wall material for the cyclodextrine that agrees with contemporary tea processing technology easily.
With the microcapsules that cyclodextrine is made, bead diameter is less, and the bright beverage of full impregnated is made in the extract mixture preparation that is fit to originate with the various plants of cyclodextrine embedding;
With the microcapsules that sodium alginate is made, bead diameter is bigger, be suitable for to make beverage like the outstanding pearl sample pearl milk tea.
Be the core of claim of the present invention with the silk gum microencapsulation, with regard to this protein of silk gum being carried out microencapsulation, the present invention does not relate to the interests of any patented technology or prior art; The present invention utilizes ripe known Technology of Making Microcapsule to reach the purpose of silk gum microencapsulation, and these Technology of Making Microcapsule are not within this claim.
Advantage of the present invention is:
The present invention has expanded the new source of food additives.
The present invention has opened up silk gum and has used new way.
The present invention has developed discarded silk gum resource, meets the national environmental protection industrial policy.
The present invention has classic of TCM foundation.
The present invention has the modern science foundation.
The present invention has big potential market.
The present invention possesses novelty.
The present invention possesses creativeness.
The present invention possesses practicality.
The specific embodiment
Example one:
Allotment concentration is the silk gum aqueous solution of 1%-15%.
Allotment cyclodextrine β-CD is mixed with 10%～50% aqueous solution.
Silk gum liquid mixes with cyclodextrine, the mass ratio of silk gum and β-CD (0.4～1): 1.
With mixed liquor homogeneous, emulsification.
Spray-drying, 200 ℃ of inlet temperatures, 118 ℃ of flow velocity 22cm/min of outlet temperature.
Obtain silk gum β-CD microcapsules, divide packing, warehouse-in.
Example two:
Allotment concentration is the silk gum aqueous solution of 1%-15%.
Allotment concentration is the 1%-5% sodium alginate aqueous solution.
Allotment concentration is the 1%-10% calcium chloride CaCl2 aqueous solution.
Allotment concentration is the 0.1%-2% chitosan-acetic acid solution, and the pH value is 4.5-7.
Allotment concentration is the 0.1%-1% glutaraldehyde water solution.
Chitosan-acetic acid solution, calcium chloride solution mix.
Silk gum liquid mixes with sodium alginate soln.
Silk gum liquid and sodium alginate soln are slowly splashed in chitosan-acetic acid solution, the CaCl2 mixed solution with syringe needle, stir with rotating speed 200r/min.
Add the finite concentration glutaraldehyde, stir fast, the microcapsules bulb diameter of formation is 2～3mm.
Obtain the silk gum microcapsules, place 4 ℃ of environment immobilization 10h.
Being fixed silk gum sodium alginate micro gel capsule divides packing, warehouse-in.
Example three:
Allotment concentration is the silk gum aqueous solution of 1%-15%.
Allotment concentration is 1%-5% sodium alginate aqueous solution A.
Allotment concentration is the 100mmol/L calcium chloride CaCl2 aqueous solution.
Allotment concentration is the 0.05% poly-D-lysine aqueous solution.
Allotment concentration is 0.14% sodium alginate aqueous solution B.
Allotment concentration is the 55mmol/L aqueous citric acid solution.
Silk gum liquid mixes with sodium alginate aqueous solution A, makes 1%-15% concentration suspension.
By the gas generator for microcapsules, spray in the CaCl2 solution, form sodium alginate calcium pearl.
Wrap up with poly-D-lysine.
React with sodium alginate aqueous solution B.
Handle with natrium citricum.
Being fixed silk gum sodium alginate poly-D-lysine microcapsules divide packing, warehouse-in.
List of references
1. leaf is built the state, 2005 the 28th volumes of the biologically active of Bai Xingrong silk gum and hydrolysate thereof and Advance on Pharmacological Activities Yunnan University of Traditional Chinese Medicine journal the 1st phase 64-67
2. open from (1156--1228) " Confucian's Duties to Their Parents " just and quench one's thirst 35
3. Wang Zhuo, domestic silkworm silk rubber powders such as Zhang Zhiqiang are to 2011 the 24th volumes of research Shenyang army medicine the 3rd phase 153-155 page or leaf for the treatment of diabetes effect
4. pay beautifully, silk gum such as horse bezoar monarch influence Chengde Medical College's journal 2008 the 25th volume the 4th phase 351-353 page or leaf to the diabetes B rat blood sugar
5. Song establishes an army, and silk gum such as Li Jian were rolled up the 2nd phase 183-187 page or leaf in 2011 the 34th to the anatomy magazine that influences of diabetic nephropathy rat kidney VEGF and pigment epidermal derived factors expression
6. pay beautiful Ma Hongwei etc., silk gum is to 2010 the 33rd volumes of protective effect anatomy magazine the 2nd phase 161-164 page or leaf of type ii diabetes rat Islet cells
7. the beautiful Rong of clock, Fu Wenliang etc., silk gum influence 2010 the 27th volumes of Chengde Medical College's journal the 1st phase 5-8 page or leaf to what diabetes B rat spinal cord anterior angle neuron NGF expressed
8. Zhan is eternally happy, and cocoon shell water decoctions such as Huang Chunfang are to the hypoglycemic activity silkworm industry science 2003 of alloxan diabetes model mice; 29 (4) 446 pages
9. Li Jisheng, 98 pages of the pretty silk cocoon extract of horse bezoar blood sugar decreasing effect Primary Study Chengde Medical College journal 2010 the 27th the 1st phases of volume
10. the king is brave certainly, the hair fire hose etc., 2000 the 15th volumes of the experimental study Chinese Medicine journal of silk cocoon hypoglycemic activity the 4th phase 21-22 page or leaf
11. the king is from brave, mao fire hose etc., the mechanism research Zhejiang College Of Traditional Chinese Medicine journal of silk cocoon hypoglycemic activity 2003 2 were the 27th volume the 1st phase 53-54 pages or leaves
12. ten thousand one-tenth of grandsons, Jiang Duxiao etc., shitosan/sodium alginate micro gel capsule immobilized phospholipase A1 and structure thereof are investigated Food Science 2005, Vol.26, No.9:100-103
13. the research of Cao Xin will cyclic dextrin clathrate glutathione China's flavouring the 12nd phase: 10-11 in 1996
14. Wang Ya light Ma Yunsheng etc., release China's Tissue Engineering Study of sodium alginate-poly-D-lysine-sodium alginate micro-capsule inner cell concentration and insulin and C peptide and clinical rehabilitation the 15th volume the 3rd phase: 567-570.
Claims (5)
1. a silk gum microcapsules production method specifically, is a kind of preparation method of microencapsulation silk gum, it is characterized in that silk gum is made the microencapsulation product that can be used for processing beverage or oral liquid.
2. according to the method that proposes in the claim 1, it is characterized in that: the silk gum that adopts is from silkworm (silkworm Bombyx mori).
3. the method that proposes according to claim 1, it is characterized in that: used high concentration silk gum liquid derives from one of following technology:
A: pure silk glue finished product, it is formulated to add water;
B: only secrete the silk gum that the silkworm kind of silk gum spues, modulation forms;
C: no auxiliary agent mulberry silk glue, come unstuck when the vacuum infiltration method of adding assistant or cooking process do not make silkworm (Bombyx mori) silk cocoon (certified products silk cocoon, substandard products damaged cocoon, longly tell, recotti, husks, plane silk or clip cocoon) throwing or throwing cotton-wadded quilt or silk scouring obtains, through the silk gum aqueous solution of removal of impurities, degerming or concentrate glue, the cream attitude silk gum semi-finished product that form again;
D: auxiliary agent mulberry silk glue is arranged, go auxiliary agent to handle the back gained to the above-mentioned raw materials back of coming unstuck with conventional method, through the silk gum aqueous solution of removal of impurities, degerming or concentrate glue, the cream attitude silk gum semi-finished product that form again.
4. the method that proposes according to claim 1, with known microcapsules preparation method, make microencapsulation silk gum as cyclodextrine β-CD method, sodium alginate method, sodium alginate poly-D-lysine method and other food and the acceptable method of medicine trade, it is characterized in that: capsule heartwood material is silkworm silk gum.
5. the method that proposes according to claim 1 to 4, gained microencapsulation silk gum can be used for composite various tea beverage, Pearled tea beverage or other food or the acceptable goods of medicine trade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201210031770 CN103238849A (en) | 2012-02-13 | 2012-02-13 | Production method and application of sericin microcapsules |
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| CN 201210031770 CN103238849A (en) | 2012-02-13 | 2012-02-13 | Production method and application of sericin microcapsules |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104826563A (en) * | 2015-04-01 | 2015-08-12 | 浙江理工大学 | Preparation method of hydroxyapatite/sericin hybrid microcapsule |
| CN106085998A (en) * | 2016-06-25 | 2016-11-09 | 张静 | A kind of preparation method of the microorganism ball for preparing ε polylysine |
| CN106617103A (en) * | 2016-11-25 | 2017-05-10 | 江苏爱西施科技服务咨询股份有限公司 | Healthcare soft capsule containing silk copeptin and preparation method of healthcare soft capsule |
| CN113017129A (en) * | 2019-12-24 | 2021-06-25 | 深圳波顿香料有限公司 | High water content capsule and preparation method thereof |
-
2012
- 2012-02-13 CN CN 201210031770 patent/CN103238849A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104826563A (en) * | 2015-04-01 | 2015-08-12 | 浙江理工大学 | Preparation method of hydroxyapatite/sericin hybrid microcapsule |
| CN106085998A (en) * | 2016-06-25 | 2016-11-09 | 张静 | A kind of preparation method of the microorganism ball for preparing ε polylysine |
| CN106617103A (en) * | 2016-11-25 | 2017-05-10 | 江苏爱西施科技服务咨询股份有限公司 | Healthcare soft capsule containing silk copeptin and preparation method of healthcare soft capsule |
| CN113017129A (en) * | 2019-12-24 | 2021-06-25 | 深圳波顿香料有限公司 | High water content capsule and preparation method thereof |
| CN113017129B (en) * | 2019-12-24 | 2022-08-05 | 深圳波顿香料有限公司 | High water content capsule and preparation method thereof |
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Application publication date: 20130814 |