CN103234951B - A kind of noble metal nano particles coat photonic crystal coding microball preparation method - Google Patents
A kind of noble metal nano particles coat photonic crystal coding microball preparation method Download PDFInfo
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- CN103234951B CN103234951B CN201310115308.6A CN201310115308A CN103234951B CN 103234951 B CN103234951 B CN 103234951B CN 201310115308 A CN201310115308 A CN 201310115308A CN 103234951 B CN103234951 B CN 103234951B
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- 238000002360 preparation method Methods 0.000 title abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 14
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- 206010020751 Hypersensitivity Diseases 0.000 claims abstract description 7
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- 239000000377 silicon dioxide Substances 0.000 claims description 11
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- 238000006243 chemical reaction Methods 0.000 claims description 7
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- 125000003277 amino group Chemical group 0.000 claims description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 2
- 239000011805 ball Substances 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 2
- 230000001900 immune effect Effects 0.000 claims 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 claims 1
- VDGJOQCBCPGFFD-UHFFFAOYSA-N oxygen(2-) silicon(4+) titanium(4+) Chemical compound [Si+4].[O-2].[O-2].[Ti+4] VDGJOQCBCPGFFD-UHFFFAOYSA-N 0.000 claims 1
- 238000005253 cladding Methods 0.000 abstract description 11
- 238000002189 fluorescence spectrum Methods 0.000 abstract description 5
- 238000005286 illumination Methods 0.000 abstract description 3
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- 230000035945 sensitivity Effects 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 230000007547 defect Effects 0.000 abstract 1
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- 229920002223 polystyrene Polymers 0.000 description 7
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- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
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- 239000004005 microsphere Substances 0.000 description 4
- 230000003595 spectral effect Effects 0.000 description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 238000000985 reflectance spectrum Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 description 3
- 238000010189 synthetic method Methods 0.000 description 3
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000001354 calcination Methods 0.000 description 2
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- 239000003638 chemical reducing agent Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000013461 design Methods 0.000 description 2
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 239000002114 nanocomposite Substances 0.000 description 2
- 239000011022 opal Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 238000001338 self-assembly Methods 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000006557 surface reaction Methods 0.000 description 2
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- 229910004042 HAuCl4 Inorganic materials 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
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- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 1
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 1
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- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920000120 polyethyl acrylate Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
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- 229920002554 vinyl polymer Polymers 0.000 description 1
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Abstract
The present invention relates to a kind of noble metal nano particles cladding photonic crystal coding microball preparation method.The present invention on the surface of photonic crystal coding microball after carrying out chemical bond modification, noble metal nano particles are coated on photonic crystal coding microball surface using the method for fabricated in situ, noble metal nano particles are introduced on photonic crystal coding microball surface to realize strengthening Surface Raman Spectra effect, using the feature reflection peak of photonic crystal coding microball, various testing samples are detected simultaneously, the unmarked multi-element biologic immune detection of hypersensitivity is realized.Cause stability not high instant invention overcomes easily being limited by illumination, temperature, storage time etc. existing for fluorescence spectrum, the problems such as biocompatibility, and the defect such as solvent effect on environment.Sensitivity degree of the present invention, detection is simple and quick, and with low cost, property is sufficiently stable, and detection efficiency is improved.
Description
Technical field
The present invention relates to the technical field of biomedical research, environmental monitoring and clinical detection, more particularly to a kind of your gold
Category nano-particle cladding photonic crystal coding microball preparation method.
Background technology
With the fast development of progressively implementation and molecular biology etc. the related discipline that human genome (sequencing) is planned,
Increasing animals and plants, microbial genome sequence are determined, and gene sequence data is fast at an unprecedented rate
Speed increases.Meanwhile, all it is often to detect many index in a sample, particularly new disease in clinical immunology detection
Pathogenic microorganism is constantly found that the new amynologic index related to disease is also constantly being found, and clinical detection project also must
Can so be continuously increased.Therefore, setting up new hybridization and sequence measurement so as to realize carrying out substantial amounts of hereditary information, clinical sample
Efficiently, quickly detection, analysis just seem increasingly important.
At present, flowing code carrier due to its preparation it is simple, encoding amount is big, receives significant attention the features such as react quick.
Photonic crystal coding wherein based on spectral technique is easy to detect to be intuitively able to universal due to its coding, detection technique maturation
Using.Additionally, relative to the carrier of other solid phase forms, microballoon has more significant advantage：First, the specific surface area ratio of microballoon
It is larger, by increasing capacitance it is possible to increase the ratio between the surface area and volume of effecting reaction, therefore the chemical reaction on surface can be made smaller
Carried out in volume range；The carrier for secondly, being used as solid phase form using microballoon can utilize related supplementary means as stirred,
Ultrasound, liquid wash away etc. to realize the reaction system of multi-form, such that it is able to accelerate the reaction speed of system；Finally, with
The change of microsphere surface functionalization group, can extend the purposes of microballoon.
But, fluorescence spectrum is relied primarily on currently with the multivariate detection of photonic crystal coding microball.The suction of fluorescence spectrum
Peak is received, the chemical constitution of molecule, the i.e. chemical constitution by molecule in itself is typically from and is determined, therefore be highly prone to illumination,
The limitation of the factors such as temperature, storage time, therefore stability is not high, usual spectrum peak scope is relatively wide to be difficult to differentiate between, and
There are problems that, additionally, the detection of fluorescence spectrum is generally required being carried out in specific solvent, solvent may
Fluorescence to material produces important ambient influnence, can make fluorescent weakening, even disappears completely, therefore is typically made using fluorescence spectrum
Also to there is certain limitation in the foundation of detection.
The content of the invention
It is an object of the invention to provide drawbacks described above is overcome, develop a kind of noble metal nano particles cladding photonic crystal and compile
Code method for preparing microsphere.
The technical scheme is that：
A kind of noble metal nano particles coat photonic crystal coding microball preparation method, and it is mainly characterized by and passes through
After the surface of photonic crystal coding microball carries out chemical bond modification, using the method for fabricated in situ by noble metal nano particles bag
Photonic crystal coding microball surface is overlayed on, i.e., introduces noble metal nano particles on photonic crystal coding microball surface to realize enhancing
Surface Raman Spectra effect, using the feature reflection peak of photonic crystal coding microball, while detecting various testing samples, realizes super
Highly sensitive unmarked multi-element biologic immune detection.
Further technical scheme of the invention is：
(1) the photonic crystal coding microball of different coding is chosen, its surface is processed, with microsphere surface functionalization
The change of group, obtains the photonic crystal coding microball with amino group modification；
(2) using the method for fabricated in situ, deionized water and contain to being added in the container for filling photonic crystal coding microball
The precursor solution of precious metal ion, the reducing agent made configured in advance is added after slow intensification, that is, obtain noble metal nano grain
The photonic crystal coding microball that attached bag is covered；
(3) hot conditions are placed in after the photonic crystal coding microball washing of the noble metal nano particles cladding that will be prepared
Lower calcining, for removing the impurity introduced in experimentation, and further enhances the mechanical strength of photonic crystal coding microball.
In the middle of the technical program, the coding of described photon crystal micro-ball is the feature in photonic crystal reflectance spectrum
The wavelength values of reflection peak.The structure of described photon crystal micro-ball is the albumen of colloidal particle long-range order ground self assembly
The photonic crystal of stone structure；The photon crystal micro-ball of opal structural has the ability of the light of reflection specific wavelength, its reflected light
Spectrum has its feature reflection peak, and wave-length coverage covers ultraviolet, it is seen that with infrared region.
The present invention introduces noble metal nano particles by chemical method on photon crystal micro-ball surface, such that it is able to be had
The nano composite material of standby three-dimensional structure, as a kind of new and with the substrate of enhancing surface-enhanced raman scattering, has concurrently
The property of the optics of noble metal nano particles, electricity, catalysis and the aspect such as immune, has photon crystal micro-ball as substrate again
Flowing code carrier characteristic, can simultaneously detect various testing samples, realize the multi-element biologic immune detection of hypersensitivity.
The present invention has advantages below：
1) noble metal nano particles cladding photonic crystal coding microball, can be micro- by choosing the photonic crystal of different coding
Ball carries out efficient coding to testing sample, meets detect multiple indexs simultaneously the need for, additionally, in photonic crystal coding microball table
Face introduces noble metal nano particles to realize strengthening Surface Raman Spectra effect, can effectively be increased using SERS
The sensitivity of strong detection, realizes the multi-element biologic immune detection of hypersensitivity；
2) this noble metal nano particles cladding photonic crystal coding microball, both can effectively using photon crystal micro-ball
Feature reflection peak learns the species of testing sample, can be detected using the intensity of SERS again and treat test sample
The content of product.The detection process is simple, and quickly.
3) this noble metal nano particles cladding photonic crystal coding microball preparation method is simple, is compiled by photonic crystal
After the surface of code microballoon carries out chemical bond modification, noble metal nano particles are coated on photonic crystal using the method for fabricated in situ
Coding microball surface, the synthetic method is easily controlled, and cost of manufacture is cheap, simple efficient；
4) this code used good stability of photonic crystal coding microball, and after noble metal nano particles are coated with
The coding efficiency of photonic crystal coding microball is consistent.The photonic crystal coding microball that the noble metal nano particles are coated is put
In under normal temperature, the stable chemical nature of its own is difficult to be influenceed by external conditions such as temperature, illumination, humidity, and property is very steady
It is fixed.The coding of stabilization is remained during application；
5) detecting step is simply efficient, because the outer layer of photonic crystal coding microball is coated by noble metal nano particles, and
General biological sample contains amino again, therefore need not can be fixed on microballoon with by probe molecule by rhetorical function group again
Surface, the step of reduce chemical coupling, improves detection efficiency.
Other specific advantages of the invention and effect will go on to say below.
Specific embodiment
Technical thought of the invention is：
Noble metal nano particles are introduced on photonic crystal coding microball surface by chemical method, such that it is able to be possessed
The nano composite material of three-dimensional structure.Test result indicate that, the photonic crystal coding microball particle size range as obtained in this method
Can control at 50 μm between 3mm, the particle size range of noble metal nano particles can be controlled between 3nm to 70nm.Its system
Preparation Method is simply efficient, and method in photonic crystal coding microball Surface coating noble metal nano particles it is relatively easy and
Size, pattern, dispersiveness can be adjusted suitably.
Experimentation of the invention is described in detail below, it is intended to make design cycle of the invention, purpose of design
And its innovative point and advantage more understand.
A kind of noble metal nano particles coat the preparation method of photonic crystal coding microball, are the photons for choosing different coding
Crystal microballoon, after carrying out chemical bond modification to its surface, is coated noble metal nano particles by the method using fabricated in situ
On the surface of photon crystal micro-ball, using the various testing samples of surface Raman enhancement spectral detection, many of hypersensitivity are realized
First biological immune detection.
Preparation method is comprised the following steps：
The photon crystal micro-ball of different coding is chosen, its surface is processed, obtain the photon with amino group modification
Crystal coding microball.Wherein photonic crystal coding microball can be organic polyvinyls photon crystal micro-ball, i.e. polyphenyl
Ethene, polypropylene, polyacrylonitrile, polymethyl methacrylate, polyethylene or polyethyl acrylate, or for inorganic photon crystal is micro-
Ball is silica or titanium dioxide.
Using the method for fabricated in situ, to addition deionized water in the container for filling photonic crystal coding microball and containing your gold
Belong to the precursor solution of ion, the reducing agent made configured in advance is added after slow intensification, that is, obtain noble metal nano particles bag
The photonic crystal coding microball for covering.Noble metal nano particles can be golden nanometer particle, Nano silver grain or nano platinum particle.
It is placed under hot conditions after the photonic crystal coding microball washing of the noble metal nano particles cladding that will be prepared
Calcining, for removing the impurity introduced in experimentation, and further enhances the mechanical strength of photonic crystal coding microball.
In the middle of the technical program, the coding of described photon crystal micro-ball is the feature in photonic crystal reflectance spectrum
The wavelength values of reflection peak.The structure of described photon crystal micro-ball is the albumen of colloidal particle long-range order ground self assembly
The photonic crystal of stone structure；The photon crystal micro-ball of opal structural can reflect the light of specific wavelength, and its reflectance spectrum has
Its feature reflection peak, wave-length coverage covers ultraviolet, it is seen that with infrared region.Described Surface coating has the light of noble metal nano particles
Sub- crystal coding microball, is to realize strengthening surface Raman light by introducing noble metal on photonic crystal coding microball surface
Spectrum effect.
Here is specific embodiment.
Embodiment 1：
The preparation of the silica photonic crystal coding microball of golden nanometer particle cladding
1st, the silica photonic crystal coding microball of different coding is chosen respectively, is dipped in 70% (V/V0) concentrated sulfuric acid
With in 30% hydrogen peroxide solution overnight so that the silica photonic crystal coding microball surface of preparation carry oh group.
The silica photonic crystal coding microball that hydroxyl will have been modified is immersed in concentration for the ethoxy of 0.015mol/L3- aminopropyls three
In the aqueous isopropanol of base silane (APTES), shaken in 80 DEG C of water bath with thermostatic control 2 hours, fully use ultra-pure water after reaction
Flush three times, unnecessary APTES is removed, silica photonic crystal coding microball surface is carried amino group.
2nd, the silica photonic crystal coding microball that will modify amino is immersed in 1% gold chloride (HAuCl4) solution
In, and slowly heat up up to boiling, 1% sodium citrate aqueous solution made configured in advance is added, it is stirred vigorously until final
Solution becomes claret, and ultrapure water is used three times after being cooled to room temperature, finally gives the silica of golden nanometer particle cladding
Photonic crystal coding microball.
3rd, the photonic crystal coding microball that will be prepared by above-mentioned condition is calcined under being placed in hot conditions, for removing
The impurity introduced in experimentation, and further enhance the mechanical strength of photonic crystal coding microball.The simple synthetic method, it is micro-
Ball is stable in properties.It is many using surface Raman enhancement spectral detection using the feature reflection peak of photonic crystal coding microball as coding
Testing sample is planted, the multi-element biologic immune detection of hypersensitivity is realized.
Embodiment 2：
The preparation of the polystyrene photon crystal coding microball of Nano silver grain modification
1st, the polystyrene photon crystal coding microball of different coding is chosen respectively, because the polystyrene photon for preparing is brilliant
Body microsphere surface carries carboxyl, therefore is placed directly within concentration for 0.015mol/L3- aminopropyltriethoxywerene werenes
(APTES) in aqueous isopropanol, shaken in 80 DEG C of water bath with thermostatic control 2 hours, fully use ultrapure water three after reaction
It is secondary, unnecessary APTES is removed, polystyrene photon crystal coding microball surface is carried amino group.
2nd, will modify amino polystyrene photon crystal coding microball be immersed in 0.2mg mL silver nitrate (AgNO3)
In solution, and slowly heat up until boiling, add 1% sodium citrate aqueous solution made configured in advance, be stirred vigorously up to
Reaction is complete, and ultrapure water is used three times after being cooled to room temperature, and the polystyrene photon for finally giving Nano silver grain cladding is brilliant
Body coding microball.
3rd, the photonic crystal coding microball that will be prepared by above-mentioned condition is calcined under being placed in hot conditions, for removing
The impurity introduced in experimentation, and further enhance the mechanical strength of photonic crystal coding microball.The simple synthetic method, it is micro-
Ball is stable in properties.It is many using surface Raman enhancement spectral detection using the feature reflection peak of photonic crystal coding microball as coding
Testing sample is planted, the multi-element biologic immune detection of hypersensitivity is realized.
Claims (2)
1. unmarked multi-element biologic immunologic detection method, it is characterised in that by the surface of photonic crystal coding microball
After learning key modification, noble metal nano particles are coated on photonic crystal coding microball surface using the method for fabricated in situ, that is, existed
Photonic crystal coding microball surface introduces noble metal nano particles to realize strengthening Surface Raman Spectra effect, using photonic crystal
The feature reflection peak of coding microball, while detecting various testing samples, realizes that the unmarked multi-element biologic of hypersensitivity is immunized
Detection；Wherein concretely comprising the following steps for chemical bond modification is carried out on the surface of photonic crystal coding microball choose different coding respectively
Silica photonic crystal coding microball, is dipped in 70% (V/V0) in the concentrated sulfuric acid and 30% hydrogen peroxide solution overnight, make
The silica photonic crystal coding microball surface that must be prepared carries oh group, and the silica photon that will modify hydroxyl is brilliant
Body coding microball is immersed in the aqueous isopropanol that concentration is 0.015mol/L APTESs, at 80 DEG C
Water bath with thermostatic control in shake 2 hours, fully unnecessary APTES is removed with ultrapure water three times after reaction, make titanium dioxide
Silicon photonic crystal coding microball surface carries amino group.
2. unmarked multi-element biologic immunologic detection method according to claim 1, it is characterised in that photonic crystal coding is micro-
, at 50 μm between 3mm, the particle size range of noble metal nano particles is between 3nm to 70nm for ball particle size range.
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| CN105652347A (en) * | 2016-01-21 | 2016-06-08 | 扬州大学 | Preparation method of three-dimensional ordered titanium dioxide photonic crystal microballoons covered by silver nanoparticles |
| CN106950616B (en) * | 2017-05-22 | 2019-11-01 | 扬州大学 | A kind of preparation method of compound Raman photon crystal microballoon |
| CN108344713B (en) * | 2018-02-06 | 2021-04-13 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Photonic crystal sensing material and preparation method and application thereof |
| CN109342392B (en) * | 2018-11-09 | 2021-09-17 | 中国科学院烟台海岸带研究所 | Surface-enhanced Raman scattering nano probe taking polystyrene as coating shell material and preparation method thereof |
| US20210394440A1 (en) * | 2020-06-18 | 2021-12-23 | University Of Massachusetts | Fabrication of structure from lost base material |
| CN114354574A (en) * | 2022-01-11 | 2022-04-15 | 扬州大学 | Multielement SERS biological detection method based on analog enzyme signal amplification |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216429A (en) * | 2008-01-07 | 2008-07-09 | 首都师范大学 | SERS biological probe and method for making same |
| CN100565185C (en) * | 2008-01-08 | 2009-12-02 | 东南大学 | A kind of photon crystal composite encoding microsphere and preparation method |
-
2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216429A (en) * | 2008-01-07 | 2008-07-09 | 首都师范大学 | SERS biological probe and method for making same |
| CN100565185C (en) * | 2008-01-08 | 2009-12-02 | 东南大学 | A kind of photon crystal composite encoding microsphere and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| Direct Metallization of Gold Nanoparticles on a Polystyrene Bead Surface using Cationic Gold Ligands;Jun-Ho Lee et al.;《Macromolecular Rapid Communications》;20071231;第28卷;634-640 * |
| 聚合物微球/微囊负载金属纳米微粒的制备及其性能研究;刘巍;《南开大学硕士学位论文》;20101124;39 * |
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