CN103224897A - Bacillussubtilis for tobacco black shank prevention and control - Google Patents
Bacillussubtilis for tobacco black shank prevention and control Download PDFInfo
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Abstract
The invention relates to a strain of Bacillussubtilis TBSCQ057 providing biocontrol activity for tobacco black shank, and belongs to the field of agricultural plant disease biocontrol. The Bacillussubtilis TBSCQ057 is separated from a natural environment host plant, is identified as the Bacillussubtilis according to morphological characteristics and molecular biology, provides a strong inhibition effect for tobacco black shank bacterial, provides a good control effect for tobacco black shank, has broad spectrum antagonism activity, and provides good bacterial inhibition effects for tobacco botrytis cinerea, tobacco alternaria alternate, tobacco sclerotium rolfsii, tobacco anthracnose pathogen, and a lot of pathogenic fungi. Secondary metabolites of the strain contain cellulose, protease, siderophore and the like. The TBSCQ057 bacterial agent provides a good control effect for the tobacco black shank, environmentally friendly bio-pesticides can be produced through bacterial fermentation or a secondary metabolite extraction technology, and important commercial development and application values are provided.
Description
Technical field
The present invention relates to the biocontrol of plant disease technical field, particularly relate to the subtilis that a strain is used to prevent and treat black shank.
Background technology
Tobacco is a kind of important cash crop, and black shank is one of the important disease in each tobacco planting district, the world.All there is generation in China most of tobacco planting district, and it is serious in a plurality of provinces and cities harm to take place.In order to cater to industrial demand to the characteristic quality, the tobacco bred of plantation has many disease resistance degree not high in the production.At present to the control of balck shank still based on chemical agent, thereby cause consequences such as the enhancing of pathogenic bacteria resistance, environmental pollution and tobacco leaf heavy metal content exceed standard, influence prevention effect, ecological safety and human health.
Summary of the invention
The purpose of this invention is to provide the subtilis TBSCQ057 that a strain is used to prevent and treat black shank
,A kind of endogenetic bacteria that obtains that separates from healthy cigarette strain.From the plant microecology system perspective, utilize the comprehensive action of endogenetic bacteria, and control the black shank evil with this to aspects such as cigarette strain rhizosphere ecology, plant Physiology and biochemistry and pathogenic bacterias.Bacterial strain TBSCQ057 can promote soil mineralization and plant to nutrient absorbing, can significantly reduce fungi quantity in the soil, improves bacterial number, especially can improve fixed nitrogen, phosphorus decomposing and potassium decomposing 3 big function bacterial numbers, and the cigarette seedling is had good growth-promoting functions.Cultivate the antagonism test through the flat board face-off, this bacterial strain has very strong restraining effect to multiple pathogenic bacterias such as tobacco black shank bacteriums, through greenhouse pot culture and field plot trial, shows that bacterial strain TBSCQ057 has good prevention effect to black shank.
TBSCQ057 strains separation purifying and screening: gather healthy cigarette strain, sample is carried out surface sterilization handle, de-epithelization cuts interior tissue and smashs to pieces then, gets to leave standstill liquid and be applied to beef broth peptone flat board, chooses colonies typical, purifying.Again on oat medium with the tobacco black shank bacterium cultivation that stands facing each other, obtaining has stronger inhibiting bacterial strain to black shank bacterium.
The morphological specificity of TBSCQ057 bacterial strain is identified: bacterial strain TBSCQ057 colony edge on the NA substratum is irregular, flat, oyster white, drying, tarnish; Gram-positive, gemma oval, middle life, peritrichous.The microscopic examination thalline is unicellular, shaft-like, single give birth to or twin, and chaining sometimes, size is 0.8～1.2 μ m * 1.9～4.9 μ m; Litmus milk produces alkali, peptonizes, and it is positive that sugar-fermenting produces acid, Citrate trianion, catalase, V-P reaction, starch hydrolysis, nitrate reduction reaction, gelatine liquefication etc.; Egg yolk reaction, hippurate, tyrosine, propionic salt and phenylalanine deaminase reaction are all negative; Can on the dextrose culture-medium that contains 5%, 7% NaC1 and pH 5.7, grow.According to these characteristics, with the TBSCQ057 preliminary evaluation be subtilis (
Bacillus subtilis).
TBSCQ057 bacterial strain Molecular Identification: extract total DNA of bacterial strain TBSCQ057, the sample gene group dna solution dilution of being extracted is carried out pcr amplification as template, pcr amplification adopts bacterium universal primer fD1 and rP1.fD1：
5'-AGAGTTTGATCCTGGCTC?AG-3'，rP1：5'-ACGGTTACCTTGTTA?CGACTT-3'。Genomic dna with bacterial strain TBSCQ057 is a template, and PCR increases after electrophoresis detection obtains the band that the l bar is about 1.5 kb, determines that through sequencing analysis 16S rDNA fragment length is 1397kb.The combining form feature is defined as subtilis with the TBSCQ057 bacterial strain
Bacillus subtilis
The live body pure culture of TBSCQ057 bacterial classification has been preserved in ' China Committee for Culture Collection of Microorganisms common micro-organisms center ' (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on 06 15th, 2012, Institute of Microorganism, Academia Sinica, postcode 100101, phone: 010-64807355), preserving number: CGMCC No. 6223.
The TBSCQ057 bacterial strain is to the restraining effect of tobacco black shank bacterium: endogenetic bacteria TBSCQ057 has very strong restraining effect to black shank bacterium in flat board face-off culturing process, the expansion of bacterium colony ovalization.Microscopic examination finds that mycelia distortion, branch increase.Scanning electron microscopic observation is found the mycelia deformity, expand and branch short and thick, the little knob of the formation that has, majority shows short and thick tubercle.
The TBSCQ057 bacterial strain is to the prevention effect of black shank: black shank is that a kind of soil passes the tubers disease, and pathogenic bacteria is mainly invaded the host by root and basal part of stem.The present invention is directed to the route of entry of pathogenic bacteria, utilize the TBSCQ057 fermented liquid to carry out root irrigation, obtained good prevention effect.The TBSCQ057 fermented liquid is 69.28% to the greenhouse prevention effect of black shank; Sub-district, the field prevention effect in 2 years is respectively 61.25% and 72.49%, with the prevention effect of contrast chemical agent 58% first frost mancozeb wettable powder do not have significant difference (
P0.05).
The optimal conditions of fermentation of TBSCQ057 bacterial strain: the single factor of strain fermentation condition is measured and shown, bacterial strain TBSCQ057 growth and suppress the suitableeest single factor condition that the antagonistic substance of black shank bacterium produces and be respectively: leavening temperature is that 28 ℃, the initial pH value of nutrient solution are 7.0, fermentation time is 48 h, shakes bottle rotating speed 180 r/min and 25 mL/500mL triangular flask liquid amounts.Carrying out optimal conditions of fermentation behind the optimization of orthogonal test is combined as leavening temperature and is that 28 ℃, the initial pH value of nutrient solution are 7.0, fermentation time is 72 h, shakes bottle rotating speed 180 r/min and 25 mL/500 mL triangular flask liquid amounts.
The antimicrobial spectrum analysis of TBSCQ057 bacterial strain: this bacterial strain can suppress the various plants pathogenic fungi, as fusarium graminearum, tobacco southern blight bacterium, tomato early blight bacterium, tobacco ash arrhizus bacteria, corn sheath blight fungus, verticillium wilt of cotton bacterium, cercospora black spot of peanut bacterium, tobacco brown spot pathogen and Colletotricum destructivum bacterium, the TBSCQ057 bacterial strain all shows restraining effect, and 4 kinds of pathogenic fungies of tobacco (tobacco brown spot pathogen, Colletotricum destructivum bacterium, tobacco ash arrhizus bacteria and tobacco southern blight bacterium) are all had stronger restraining effect.
The antagonism meta-bolites of bacterial strain TBSCQ057 is analyzed: this bacterial strain of test shows can produce cellulase, and proteolytic enzyme is had a liking for the iron element, but does not produce chitinase.
Advantage of the present invention
From healthy cigarette strain, separate the endophytic Bacillus subtilis TBSCQ057 bacterial strain that obtains, it is a new bacterial strain of finding first and identifying, itself has very strong restraining effect to various plants pathogenic bacterias such as tobacco black shank bacteriums on flat board, its fermented liquid has good prevention and control effect to black shank.Because this bacterial strain is to obtain inner the separation from the cigarette strain, show that this bacterial strain can grow surely in cigarette strain inside, and this bacterial strain strain has growth-promoting functions to cigarette.Simultaneously, this bacterial strain and the little ecology of cigarette strain have natural harmonious blending, compare with chemical pesticide that soil ecology has no side effect, noresidue, therefore, have potential business development and using value in the biological control practice of disease.
Description of drawings
Fig. 1 is a bacterial strain TBSCQ057 colonial morphology;
Fig. 2 is shaft-like thalline of bacterial strain TBSCQ057 and peritrichous;
Fig. 3 is the PCR amplification of bacterial strain TBSCQ057 16S rDNA; M:100bp DNA gradient Marker; Swimming lane 1-6:Itb57; Swimming lane 7,8: positive control; Swimming lane 9: negative control; Swimming lane 10: blank
Fig. 4 is the 16S rDNA extension increasing sequence of bacterial strain TBSCQ057;
Fig. 5 is the inhibition effect (left side: suppressed bacterium colony, the right side: normal bacterium colony) of bacterial strain TBSCQ057 to tobacco black shank bacterium;
Fig. 6 is the greenhouse prevention effect (A: pathogenic bacteria handle, B: first frost MnZn handle C: bacterial strain TBSCQ057 handle, D: clear water handle) of bacterial strain TBSCQ057 to black shank;
Fig. 7 be bacterial strain TBSCQ057 to tobacco brown spot pathogen (
Alternaria alternate), tobacco southern blight bacterium (
Sclerotium rolfsii), the tobacco ash arrhizus bacteria (
Botrytis cinerea) and the Colletotricum destructivum bacterium (
Colletotrichum nigrum) the inhibition effect;
Fig. 8 is that the antagonism meta-bolites of bacterial strain TBSCQ057 detects the A. chitinase, the B. cellulase, and C. proteolytic enzyme, D. are had a liking for the iron element.
Embodiment
Below in conjunction with embodiment the present invention is further described
The acquisition of embodiment 1 bacterial strain TBSCQ057 and correlated character analysis
1.1 the separation and purification of bacterial strain TBSCQ057: gather healthy cigarette strain, sample is carried out conventional surface sterilization handle, de-epithelization cuts interior tissue and smashs to pieces then, gets to leave standstill liquid and be applied to beef broth peptone flat board, chooses colonies typical, purifying.Again on oat medium with the tobacco black shank bacterium cultivation that stands facing each other, obtaining has stronger inhibiting TBSCQ057 bacterial strain to black shank bacterium.
1.2 bacterial strain TBSCQ057 is to the influence of tobacco seedling growth: the bacterial strain TBSCQ057 colony inoculation that will be kept on the slant medium carries out fermentation culture in the NB nutrient solution, obtains bacteria suspension, again bacteria suspension is diluted to 3 * 10
8Cfu/mL.Get bacteria suspension and carry out following 4 kinds of processing: A(processing of soaking seed), B(seed soaking+root irrigation), the C(root irrigation of spraying), D(blank-handle with sterilized water seed soaking, vernalization).Every subsequently alms bowl by identical grain apart from 5 of sowings, wait to sprout neat after, keep every alms bowl 2 strain seedling, every processing repeats for 4 times.The cigarette seedling of treatments B was irritated root, every alms bowl consumption 10 mL with bacteria suspension on the 30th day after the thinning; The cigarette seedling of handling C is squirted the blade face earlier will remain the bacterium liquid irrigating root again, every alms bowl consumption 10 mL; The cigarette seedling of handling A, D is carried out root irrigation with sterilized water, every alms bowl consumption 10 mL.All handle same light according to and water and fertilizer condition under cultivate, random alignment, every day the turned position.Be cultured to the true leaf number of measuring every alms bowl tobacco seedling on the 45th day, the length and width of maximum leaf, overground part fresh weight and dry weight.Detect the influence of bacterial strain TBSCQ057 with this to tobacco seedling growth.
1.3 bacterial strain TBSCQ057 is to the influence of cigarette shoot root border flora: bacteria suspension is handled the rhizosphere soil sampling that cultivate the back, use dilution method, soil sample is made serial dilution degree to 10
-4, the amount with every ware 50,50,100 μ l inserts in PDA, NA and the Gause I substratum respectively, and in order to measure fungi, bacterium and actinomycetes total amount, every kind of substratum of each sample is established 3 repetitions.Respectively fungi is put 25 ℃, bacterium and put 28 ℃, actinomycetes and put 30 ℃ of incubators and cultivate 2-4d, observe also meter colony number.Functional flora separates counting and adopts each selective medium and corresponding cultural method.Analyze the influence of bacterial strain TBSCQ057 with this, detect simultaneously fixed nitrogen, phosphorus decomposing and potassium decomposing 3 big function bacterial number influences to fungi, bacterium and actinomycetes quantity in the soil.
1.4 bacterial strain TBSCQ057 is to the antagonistic action of black shank: at the dull and stereotyped center inoculation of PSA tobacco black shank bacterium mycelia piece, at the both sides of anomaly plate center 3.0 cm symmetry streak inoculation bacterial strain TBSCQ057 bacteria suspension, be not contrast if do not inoculate antagonistic bacterium TBSCQ057, place 28 ℃ of incubators to cultivate.Observe antagonistic effect after 4 days.Then with blade cut downtrod Yan Cao ?shin germ colony edge mycelia piece and contrast mycelia piece.After serial scanning electron microscope example preparation process, the sample that makes is observed under scanning electron microscope.Analyze the restraining effect of this bacterial strain with this to black shank bacterium.
1.5 the evaluation of bacterial strain TBSCQ057: (1) morphological observation and evaluation: bacterial strain TBSCQ057 is cultivated down 1-3d in 28 ℃ on the NA culture plate, observe its colonial morphology (shape, color gloss, surface be bending, edge shape etc. whether); (2) molecular biology identification: the genomic dna that extracts bacterial strain TBSCQ057, with bacterium universal primer fD1:5'-AGAGTTTGATCCTGGCTC AG-3', rP1:5'-ACGGTTACCTTGTTA CGACTT-3', genomic dna with bacterial strain TBSCQ057 is a template, PCR increases after electrophoresis detection checks order; Utilize Blast software and BioEdit software on the NCBI website to carry out sequence homology analysis, with generic
Bacillus cereusBe outer cohort, (Neighbor-Joining) constructing system is grown evolutionary tree to the contiguous method of employing Mega 4.0 softwares, proves conclusively the kind level taxonomic category title of bacterial strain TBSCQ057 thus.Determine its classification position.
Example 2. bacterial strain TBSCQ057 are to the prevention effect of balck shank
2.1 the sick test of greenhouse control; Scrape the black shank bacterium mycelia of on oat medium, cultivating 14d, add 0.1%KNO
3Solution impregnation adds sterilized water behind the product spore and smashs to pieces, and adjusting concentration is about 1 * 10
4Individual sporocyst/mL dilutes 10 times behind placement 20 min down in 8 ℃, and is standby behind the glucose of adding 1%.The TBSCQ057 fermented liquid is to obtain in 2 days with fermentation in the 10-100 L automatic fermenter.Fermented liquid concentration is adjusted into about 1 * 10
8Cfu/mL.Pot experiment is used sterilization vegetable plot soil, in 10 cm that pack into * potted plant alms bowl of 10 cm, executes TBSCQ057 fermented liquid, each every strain 10 mL with the filling in preceding 10 days of inoculation pathogenic bacteria during transplanting.Contrast medicament (58% first frost mancozeb wettable powder) is irritated root with 500 times of liquid, 24 h before the inoculation pathogenic bacteria, and every strain 10 mL establish clear water and are treated to blank, and each handles 15 strains, 3 repetitions.When growing to 6-7 sheet true leaf, the cigarette seedling irritates root inoculation tobacco black shank bacterium, every strain 10 mL, and inoculation is preceding with the moistening soil of a small amount of clear water.After the processing plant is placed 24 h under 20 ℃ of dark, place 28 ℃ of hot-house cultures again, Routine Management.7 days " Invest, Then Investigate " tobacco plant incidences, statistics sickness rate and disease index are analyzed the greenhouse control effect of TBSCQ057 bacterial strain fermentation liquor to black shank with this.
2.2 field control test: select the black shank plot of morbidity throughout the year, soak root with 1500 times of liquid of the mould missible oil of 20% Dao Ling Evil during the cigarette transplantation of seedlings and handle.Handle in the following manner from transplanting beginning: with 1 * 10
8The TBSCQ057 strain fermentation liquid irrigating root of cfu/mL, every strain 250 mL, irritating root with 500 times of liquid equivalent of 58% first frost mancozeb wettable powder is the medicament contrast, establishing clear water filling root is blank.Every sub-district area 50 m
2, plant cigarette 70 strains, the sub-district random alignment, the tobacco cultivation management of conventional land for growing field crops is adopted in 3 repetitions.Investigate incidence mid-term in the tobacco leaf results, statistics sickness rate and disease index calculate the TBSCQ057 fermented liquid to the relative prevention effect in the field of black shank with this.
Example 3 bacterial strain TBSCQ057 fermentation conditions
3.1 medium pH value: the pH value that the 30g/L peanut powder nutrient solution after will sterilizing with aseptic 1mol/L NaOH or HCl is a liter is adjusted to 10 kinds of processing such as 4.0,5.0,5.5,6.0,6.5,7.0,7.5,8.0,9.0 and 11.0 respectively, and every processing repeats for 3 times.Insert the TBSCQ057 bacteria suspension by 5% inoculum size,, under 180 r/min, after dark shaking culture 48 h, fermentation culture dilute with the gradient dilution method, use the blood counting chamber number concentration to determine best pH value at 28 ℃.
3.2 culture temperature: utilize 30g/L peanut powder nutrient solution respectively 22,25,28,31,34 and 37 ℃ of cultivations, insert the TBSCQ057 bacteria suspension by 5% inoculum size, at 28 ℃, under 180 r/min, after dark shaking culture 48 h, fermentation culture dilute with the gradient dilution method.Use the blood counting chamber number concentration, every processing repeats for 3 times, to determine optimum culturing temperature.
3.3 air flow: utilizing 30g/L peanut powder nutrient solution is that 25,50,75,100 and 150 mL/500 mL triangular flasks are cultivated at liquid amount respectively, insert the TBSCQ057 bacteria suspension by 5% inoculum size, at 28 ℃, under 180 r/min, after dark shaking culture 48 h, fermentation culture dilute with the gradient dilution method.Use the blood counting chamber number concentration, every processing repeats for 3 times.To determine best air flow.
3.4 incubation time: utilize 30g/L peanut powder nutrient solution shake flask fermentation, cultivate 24,36,48,60,72,96 and 120 h respectively, insert the TBSCQ057 bacteria suspension by 5% inoculum size, at 28 ℃, under 180 r/min, after dark shaking culture 48 h, fermentation culture dilute with the gradient dilution method.Use the blood counting chamber number concentration, every processing repeats for 3 times, to determine best incubation time.
3.5 shaking speed: utilize 30g/L peanut powder nutrient solution shake flask fermentation, rotating speed is made as 90,120,150,180 and 210 r/min respectively, insert the TBSCQ057 bacteria suspension by 5% inoculum size, at 28 ℃, under 180 r/min, after dark shaking culture 48 h, fermentation culture dilute with the gradient dilution method.Use the blood counting chamber number concentration, every processing repeats for 3 times, to determine best incubation time.
3.6 fermentation condition optimization: each the single factor top condition so that preceding method records, design 5 factors as following table, 4 levels are carried out orthogonal test and are measured increment.Analyze the optimization fermentation condition of TBSCQ057 bacterial strain with this.
Table 1 factor and level
| Level | A | B | | D | E | |
| 1 | 6 | 22 | 36 | 120 | 25 | |
| 2 | 6.5 | 25 | 48 | 150 | 50 | |
| 3 | 7 | 28 | 60 | 180 | 75 | |
| 4 | 8 | 31 | 72 | 210 | 100 |
Annotate: the A-initial pH value, the B-temperature (℃), C-time (h), D-rotating speed (r/min), E-liquid amount (mL/500mL bottle).
16S rDNA gene order:
1?tgcagtcgag?cggacagatg?ggagcttgct?ccctgatgtt?agcggcggac?gggtgagtaa
61?cacgtgggta?acctgcctgt?aagactggga?taactccggg?aaaccggggc?taataccgga
121?tggttgtttg?aaccgcatgg?ttcaaacata?aaaggtggct?tcggctacca?cttacagatg
181?gacccgcggc?gcattagcta?gttggtgagg?taacggctca?ccaaggcaac?gatgcgtagc
241?cgacctgaga?gggtgatcgg?ccacactggg?actgagacac?ggcccagact?cctacgggag
301?gcagcagtag?ggaatcttcc?gcaatggacg?aaagtctgac?ggagcaacgc?cgcgtgagtg
361?atgaaggttt?tcggatcgta?aagctctgtt?gttagggaag?aacaagtacc?gttcgaatag
421?ggcggtacct?tgacggtacc?taaccagaaa?gccacggcta?actacgtgcc?agcagccgcg
481?gtaatacgta?ggtggcaagc?gttgtccgga?attattgggc?gtaaagggct?cgcaggcggt
541?ttcttaagtc?tgatgtgaaa?gcccccggct?caaccgggga?gggtcattgg?aaactgggga
601?acttgagtgc?agaagaggag?agtggaattc?cacgtgtagc?ggtgaaatgc?gtagagatgt
661?ggaggaacac?cagtggcgaa?ggcgactctc?tggtctgtaa?ctgacgctga?ggagcgaaag
721?cgtggggagc?gaacaggatt?agataccctg?gtagtccacg?ccgtaaacga?tgagtgctaa
781?gtgttagggg?gtttccgccc?cttagtgctg?cagctaacgc?attaagcact?ccgcctgggg
841?agtacggtcg?caagactgaa?actcaaagga?attgacgggg?gcccgcacaa?gcggtggagc
901?atgtggttta?attcgaagca?acgcgaagaa?ccttaccagg?tcttgacatc?ctctgacaat
961?cctagagata?ggacgtcccc?ttcgggggca?gagtgacagg?tggtgcatgg?ttgtcgtcag
1021?ctcgtgtcgt?gagatgttgg?gttaagtccc?gcaacgagcg?caacccttga?tcttagttgc
1081?cagcattcag?ttgggcactt?taaggtgact?gccggtgaca?aaccggagga?aggtggggat
1141?gacgtcaaat?catcatgccc?cttatgacct?gggctacaca?cgtgctacaa?tggacagaac
1201?aaagggcagc?gaaaccgcga?ggttaagcca?atcccacaaa?tctgttctca?gttcggatcg
1261?cagtttgcaa?ctcgactgcg?tgaagctgga?atcgctagta?atcgcggatc?agcatgccgc
1321?ggtgaatacg?ttcccgggcc?ttgtacacac?cgcccgtcac?accacgagag?tttgtaacac
1381?ccgaagtcgg?tgaggta
Claims (1)
- One strain be used to prevent and treat black shank subtilis ( Bacillus subtilis) TBSCQ057, its preserving number is CGMCC NO.6223.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571777A (en) * | 2013-10-18 | 2014-02-12 | 浙江大学 | Biocontrol strain BS102 and application thereof in preventing and treating plant gray mold |
| CN103614319A (en) * | 2013-11-12 | 2014-03-05 | 云南省烟草公司曲靖市公司 | Bacillus aryabhattai and application thereof in preventing and treating tobacco black shank |
| CN105296381A (en) * | 2015-08-31 | 2016-02-03 | 哈尔滨师范大学 | Bacillus subtilis CYY-25 and application thereof |
| CN106190890A (en) * | 2016-07-08 | 2016-12-07 | 郑州大学 | A kind of complex microbial inoculum preventing and treating black shank and biological organic fertilizer |
| CN106754557A (en) * | 2017-01-25 | 2017-05-31 | 贵州省烟草公司贵阳市公司 | Bacillus subtilis YBM 4 and its application in preventing and treating tobacco black shank and growth promotion |
| CN112358993A (en) * | 2020-11-16 | 2021-02-12 | 云南省烟草公司昆明市公司 | Bacillus subtilis MC4-2 and application thereof |
| CN116496924A (en) * | 2022-12-08 | 2023-07-28 | 中国烟草总公司湖北省公司 | Bacillus rugosus YC25 and application thereof in prevention and control of tobacco diseases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154186A (en) * | 2011-04-14 | 2011-08-17 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
| CN102747013A (en) * | 2012-05-22 | 2012-10-24 | 中国农业科学院烟草研究所 | Tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3 |
-
2013
- 2013-04-11 CN CN2013101243237A patent/CN103224897A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154186A (en) * | 2011-04-14 | 2011-08-17 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
| CN102747013A (en) * | 2012-05-22 | 2012-10-24 | 中国农业科学院烟草研究所 | Tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3 |
Non-Patent Citations (1)
| Title |
|---|
| 张慧丽 等: "一株烟草黑胫病拮抗细菌的鉴定与防效测定", 《2005年华东植物病理学术研讨会文集》 * |
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| CN103571777A (en) * | 2013-10-18 | 2014-02-12 | 浙江大学 | Biocontrol strain BS102 and application thereof in preventing and treating plant gray mold |
| CN103571777B (en) * | 2013-10-18 | 2015-06-17 | 浙江大学 | Biocontrol strain BS102 and application thereof in preventing and treating plant gray mold |
| CN103614319A (en) * | 2013-11-12 | 2014-03-05 | 云南省烟草公司曲靖市公司 | Bacillus aryabhattai and application thereof in preventing and treating tobacco black shank |
| CN103614319B (en) * | 2013-11-12 | 2015-06-17 | 云南省烟草公司曲靖市公司 | Bacillus aryabhattai and application thereof in preventing and treating tobacco black shank |
| CN105296381A (en) * | 2015-08-31 | 2016-02-03 | 哈尔滨师范大学 | Bacillus subtilis CYY-25 and application thereof |
| CN105296381B (en) * | 2015-08-31 | 2018-11-30 | 哈尔滨师范大学 | One bacillus subtilis CYY-25 and its application |
| CN106190890A (en) * | 2016-07-08 | 2016-12-07 | 郑州大学 | A kind of complex microbial inoculum preventing and treating black shank and biological organic fertilizer |
| CN106190890B (en) * | 2016-07-08 | 2019-05-14 | 郑州大学 | A kind of complex microbial inoculum and biological organic fertilizer for preventing and treating tobacco black shank |
| CN106754557A (en) * | 2017-01-25 | 2017-05-31 | 贵州省烟草公司贵阳市公司 | Bacillus subtilis YBM 4 and its application in preventing and treating tobacco black shank and growth promotion |
| CN112358993A (en) * | 2020-11-16 | 2021-02-12 | 云南省烟草公司昆明市公司 | Bacillus subtilis MC4-2 and application thereof |
| CN116496924A (en) * | 2022-12-08 | 2023-07-28 | 中国烟草总公司湖北省公司 | Bacillus rugosus YC25 and application thereof in prevention and control of tobacco diseases |
| CN116496924B (en) * | 2022-12-08 | 2023-11-03 | 中国烟草总公司湖北省公司 | Bacillus rugosus YC25 and application thereof in prevention and control of tobacco diseases |
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