CN103222392A - Production process of ganoderma lucidum mycelia - Google Patents

Production process of ganoderma lucidum mycelia Download PDF

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Publication number
CN103222392A
CN103222392A CN2012100283603A CN201210028360A CN103222392A CN 103222392 A CN103222392 A CN 103222392A CN 2012100283603 A CN2012100283603 A CN 2012100283603A CN 201210028360 A CN201210028360 A CN 201210028360A CN 103222392 A CN103222392 A CN 103222392A
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ganoderma lucidum
sterilization
mycelia
lucidum mycelium
medium
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CN2012100283603A
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廖小霞
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Abstract

The invention relates to the technical field of production processes of edible and medicinal fungi, in particular to a production process of ganoderma lucidum mycelia. The production process of the ganoderma lucidum mycelia is characterized by comprising the steps as follows: bagging culture media, sterilizing culture bags, performing inoculation, cultivating the mycelia, baking the mycelia to be dry and cooked, smashing the mycelia and packaging the mycelia. The production process is pollution-free, simple in processing method, low in cost, safe, sanitary and suitable for industrialized production. The effective activity of obtained ganoderma lucidum mycelium powder is completely kept, and the ganoderma lucidum mycelium powder has the same effect with wild ganoderma lucidum, wherein the yield of the ganoderma lucidum mycelia is larger than or equal to 60%, the content of ganoderma lucidum polysaccharide in the mycelia is 14.64%, and the content of triterpene is 1.65%.

Description

The ganoderma lucidum mycelium production technology
Technical field
The present invention relates to the edible and medical fungi technical field of producing, especially relate to a kind of ganoderma lucidum mycelium production technology.
Background technology
((Ganoderma Iucidum) has another name called polyporus lucidus to glossy ganoderma, Basidiomycetes Polyporaceae Ganoderma.Since ancient times, just that it is edible as medicine or tonic the East Asia Region people; Now, glossy ganoderma food medicine dual-purpose, the Pharmacopoeia of the People's Republic of China (2000 editions, 1 one) have recorded glossy ganoderma (red sesame and purple sesame fruit body) as legal traditional Chinese medicine: obtain Ministry of Public Health's approval in October, 2003 as new resource food; " U.S.'s herbal medicine pharmacopeia and treatment summary " also recorded glossy ganoderma.
Glossy ganoderma (Ganoderma Iucidum) is the food and medicament dual-purpose fungi, has enhance immunity, improves microcirculation, Green Tea Extract, effect such as promote longevity.Scientific research confirms, kind of compound surplus being separated to 150 in the glossy ganoderma, comprise polysaccharose, triterpenes, ucleosides, furans, alkaloids, aminoacid protein class, grease class, organic germanium, inorganic ions, sterols etc., wherein polysaccharide and triterpene two big class active substances are considered to the main pharmacodynamics composition, and its content can be used as the standard of weighing the glossy ganoderma quality.Aspects such as modern medicine study finds that main active ingredient is GL-B in the glossy ganoderma, and its health care mainly concentrates on and regulate immune, antitumor, hypotensive, reducing blood lipid, protects liver, antiviral.When edible and medical value was extensively approved glossy ganoderma, glossy ganoderma or GL-B security of products, production cost were the bottlenecks that use in restriction glossy ganoderma market.
Because the Wild ganoderma resource-constrained by the artificial cultivation glossy ganoderma, obtains ganoderma lucidum fruitbody.But the glossy ganoderma artificial cultivation tends to bring pollution, and pesticide residue is difficult to thoroughly get rid of.Fermentation method can obtain a large amount of ganoderma lucidum myceliums at short notice by regulating medium composition, cultivation temperature and time etc., shows after tested, and these mycelial chemical composition or physiological functions, similar to the ganoderma lucidum fruitbody that artificial cultivation obtains.For having overcome pollution and residue of pesticide, mainly take zymotechnique both at home and abroad, obtain ganoderma lucidum mycelium.Fermentation has two kinds of methods: a kind of is to adopt liquid fermentation technology to obtain ganoderma lucidum mycelium; Another kind is to adopt solid-fermented technique to obtain ganoderma lucidum fruitbody.Preceding a kind of liquid fermentation technology equipment investment is big, floor space is big, and particularly water consumption is quite big, and mycelial earning rate is not high yet.A kind of method solid-fermented technique in back can directly obtain ganoderma lucidum fruitbody, but fermentation period is long.
Oat has another name called bromegrass, wild wheat.Oat generally is divided into band bran type and naked grain type two big classes.The oat of countries in the world cultivations often is called the skin oat based on band bran type.The oat of China cultivation often claims naked oat based on naked grain type.Oat be China important small coarse cereals and one of economic crops.Oat and other cereal maximums different are to contain in the oat bran rich soluble and insoluble diedairy fiber; The Soluble Fiber main component is a beta glucan.The existing beta glucan that studies show that has the physiological function to the human body beneficial, as hypoglycemic, reducing blood lipid and improve immunocompetence etc.
Summary of the invention
Provide a kind of ganoderma lucidum mycelium production technology in order to overcome the prior art deficiency, pollution-free in its production process, processing method is simple, with low cost, safety and sanitation, is suitable for batch production production.
For realizing the purpose of foregoing invention, technical scheme that the present invention adopts is: a kind of ganoderma lucidum mycelium production technology is characterized in that described processing step is as follows:
(1), pack: select for use the naked oat raw material to make medium, the twice water that adds medium weight, soaked 8~24 hours, clean, filter is done, and the naked oat medium of handling well is packed in 33cm * 17cm * 0.005cm polypropylene plastics pocket, when filling with substance compresses, lasso on the sack is filled in tampon, and each culture bag weight of loading is 1000g;
(2), sterilization: 16 culture bag are placed in the sterilization frame in the lump, and 50 sterilization frames are placed on the sterilization frame in the lump, and the frame of will sterilizing pushes in the autoclaving still, and the Sterilization Kettle internal pressure requires to reach 1.2kg/cm 2, temperature is 121 ℃, sterilization time is 2 hours;
(3), inoculation: the culture bag after will sterilizing is released Sterilization Kettle and is cooled off, temperature requirement is below 30 ℃, culture bag is put into desinfection chamber to be inoculated, inoculation method is that tampon in the sack is taken out, insert lucidum strain, then tampon is filled in sack, its inoculum concentration inserts the 10g lucidum strain for every bag;
(4), cultivate: will contain the lucidum strain culture bag and put into culturing room and carry out cultural hypha, temperature is controlled under 22~28 ℃, and the time is 40~70 days, and the white hypha body is covered with whole bag culture medium;
(5), oven dry baking is ripe: take off the polypropylene plastics pocket, the medium that will contain ganoderma lucidum mycelium is put into baking oven dries ripely, and temperature is controlled between 50 ℃~90 ℃, and the time, moisture was below 8% at 3~5 hours;
(6), pulverize: will dry ripe ganoderma lucidum mycelium and be crushed into the ganoderma lucidum mycelium pulvis, grain fineness requires less than 80 orders;
(7), packing: the ganoderma lucidum mycelium pulvis is incapsulated, and every capsule 0.5g ganoderma lucidum mycelium pulvis of packing into is whenever bottledly gone into 100, can be for for oral administration.
Beneficial effect of the present invention, the naked oat raw material that adopts is made medium, and ganoderma lucidum mycelium is cultivated under aseptic condition, its heavy metal, bacterial content all meet the state food standard, pollution-free in its production process, processing method is simple, with low cost, safety and sanitation, is suitable for batch production production; The ganoderma lucidum mycelium powder that obtains, complete its effective active that kept, consistent with the Wild ganoderma effect, ganoderma lucidum mycelium yield 〉=60% wherein, ganoderma polyoses content 14.64% in the mycelium, triterpene content 1.65%, being a kind of healthy food, also is the first-class raw material of making health products.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment:
Technical scheme that the present invention adopts: described a kind of ganoderma lucidum mycelium production technology is characterized in that described processing step is as follows:
(1), pack: select for use the naked oat raw material to make medium, the twice water that adds medium weight, soaked 14 hours, clean, filter is done, and the naked oat medium of handling well is packed in 33cm * 17cm * 0.005cm polypropylene plastics pocket, when filling with substance compresses, lasso on the sack is filled in tampon, and each culture bag weight of loading is 1000g;
(2), sterilization: 16 culture bag are placed in the sterilization frame in the lump, and 50 sterilization frames are placed on the sterilization frame in the lump, and the frame of will sterilizing pushes in the autoclaving still, and the Sterilization Kettle internal pressure requires to reach 1.2kg/cm 2, temperature is 121 ℃, sterilization time is 2 hours;
(3), inoculation: the culture bag after will sterilizing is released Sterilization Kettle and is cooled off, temperature requirement is below 30 ℃, culture bag is put into desinfection chamber to be inoculated, inoculation method is that tampon in the sack is taken out, insert lucidum strain, then tampon is filled in sack, its inoculum concentration inserts the 10g lucidum strain for every bag;
(4), cultivate: will contain the lucidum strain culture bag and put into culturing room and carry out cultural hypha, temperature is controlled under 25 ℃, and the time is 55 days, and the white hypha body is covered with whole bag culture medium;
(5), oven dry baking is ripe: take off the polypropylene plastics pocket, the medium that will contain ganoderma lucidum mycelium is put into baking oven dries ripely, and temperature is controlled at 70 ℃, and the time, moisture was below 8% at 4 hours;
(6), pulverize: will dry ripe ganoderma lucidum mycelium and be crushed into the ganoderma lucidum mycelium pulvis, grain fineness requires less than 80 orders;
(7), packing: the ganoderma lucidum mycelium pulvis is incapsulated, and every capsule 0.5g ganoderma lucidum mycelium pulvis of packing into is whenever bottledly gone into 100, can be for for oral administration.

Claims (1)

1. ganoderma lucidum mycelium production technology is characterized in that described processing step is as follows:
(1), pack: select for use the naked oat raw material to make medium, the twice water that adds medium weight, soaked 8~24 hours, clean, filter is done, and the naked oat medium of handling well is packed in 33cm * 17cm * 0.005cm polypropylene plastics pocket, when filling with substance compresses, lasso on the sack is filled in tampon, and each culture bag weight of loading is 1000g;
(2), sterilization: 16 culture bag are placed in the sterilization frame in the lump, and 50 sterilization frames are placed on the sterilization frame in the lump, and the frame of will sterilizing pushes in the autoclaving still, and the Sterilization Kettle internal pressure requires to reach 1.2kg/cm 2, temperature is 121 ℃, sterilization time is 2 hours;
(3), inoculation: the culture bag after will sterilizing is released Sterilization Kettle and is cooled off, temperature requirement is below 30 ℃, culture bag is put into desinfection chamber to be inoculated, inoculation method is that tampon in the sack is taken out, insert lucidum strain, then tampon is filled in sack, its inoculum concentration inserts the 10g lucidum strain for every bag;
(4), cultivate: will contain the lucidum strain culture bag and put into culturing room and carry out cultural hypha, temperature is controlled under 22~28 ℃, and the time is 40~70 days, and the white hypha body is covered with whole bag culture medium;
(5), oven dry baking is ripe: take off the polypropylene plastics pocket, the medium that will contain ganoderma lucidum mycelium is put into baking oven dries ripely, and temperature is controlled between 50 ℃~90 ℃, and the time, moisture was below 8% at 3~5 hours;
(6), pulverize: will dry ripe ganoderma lucidum mycelium and be crushed into the ganoderma lucidum mycelium pulvis, grain fineness requires less than 80 orders;
(7), packing: the ganoderma lucidum mycelium pulvis is incapsulated, and every capsule 0.5g ganoderma lucidum mycelium pulvis of packing into is whenever bottledly gone into 100, can be for for oral administration.
CN2012100283603A 2012-01-30 2012-01-30 Production process of ganoderma lucidum mycelia Pending CN103222392A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104106372A (en) * 2014-06-23 2014-10-22 黄艳芳 Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body
CN105018532A (en) * 2014-04-28 2015-11-04 大自然生机股份有限公司 Ganoderma lucidum liquid state culture medium and application thereof
CN106579376A (en) * 2016-11-30 2017-04-26 山东瑞芝生物科技有限公司 Formula of semisolid ganoderma lucidum zymogen powder, and secondary-fermentation fully-automatic production technology
CN106852496A (en) * 2015-12-09 2017-06-16 黄建钧 The method of the triterpenes synthesis contained by induction mushroom gill fungus
CN112385507A (en) * 2020-11-16 2021-02-23 南京农业大学 Seedling culture medium containing arbuscular mycorrhiza and preparation method and application thereof
CN113999777A (en) * 2021-12-13 2022-02-01 东莞市英芝堂生物工程有限公司 Ganoderma sinensis with high polysaccharide yield and fermentation production process thereof
CN115336652A (en) * 2022-08-26 2022-11-15 韶关学院 Cultivation material and preparation method thereof, ganoderma lucidum mycelium and mulberry leaf tea and preparation method thereof

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CN101449803A (en) * 2008-12-26 2009-06-10 山西瑞芝生物科技有限公司 Ganoderma lucidum powder production method

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CN1093217A (en) * 1993-04-03 1994-10-12 张树相 Medium and mycelium are produced the method for golden mushroom powder
CN1162409A (en) * 1996-04-12 1997-10-22 曹德宾 Edible fungus powder and preparation thereof
CN1365603A (en) * 2000-10-06 2002-08-28 宋在澫 Method of culturing mycellium by using grain, its product use for culturing product
CN101449803A (en) * 2008-12-26 2009-06-10 山西瑞芝生物科技有限公司 Ganoderma lucidum powder production method

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105018532A (en) * 2014-04-28 2015-11-04 大自然生机股份有限公司 Ganoderma lucidum liquid state culture medium and application thereof
CN104106372A (en) * 2014-06-23 2014-10-22 黄艳芳 Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body
CN106852496A (en) * 2015-12-09 2017-06-16 黄建钧 The method of the triterpenes synthesis contained by induction mushroom gill fungus
CN106579376A (en) * 2016-11-30 2017-04-26 山东瑞芝生物科技有限公司 Formula of semisolid ganoderma lucidum zymogen powder, and secondary-fermentation fully-automatic production technology
CN106579376B (en) * 2016-11-30 2020-09-08 山东瑞芝生物科技股份有限公司 Formula of semisolid ganoderma lucidum zymophyte powder and full-automatic secondary fermentation production process
CN112385507A (en) * 2020-11-16 2021-02-23 南京农业大学 Seedling culture medium containing arbuscular mycorrhiza and preparation method and application thereof
CN113999777A (en) * 2021-12-13 2022-02-01 东莞市英芝堂生物工程有限公司 Ganoderma sinensis with high polysaccharide yield and fermentation production process thereof
CN115336652A (en) * 2022-08-26 2022-11-15 韶关学院 Cultivation material and preparation method thereof, ganoderma lucidum mycelium and mulberry leaf tea and preparation method thereof

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Application publication date: 20130731