CN103214317B - Active amino acid water-soluble fertilizer as well as preparation method and application thereof - Google Patents
Active amino acid water-soluble fertilizer as well as preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 27
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
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- 238000000034 method Methods 0.000 claims description 60
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Landscapes
- Fertilizers (AREA)
Abstract
The invention provides an active amino acid water-soluble fertilizer. The fertilizer is composed of the following components in parts by weight: 480-836 parts of an active compound amino acid preparation, 210-410 parts of a compound amino acid microelement chelate, 8-16 parts of a plant nutrient element and 14-26 parts of a plant activator, wherein the effective components of the active compound amino acid preparation comprise L-amino acid with biological activity and bioactive peptides, and the compound amino acid microelement chelate is formed by chelating the active compound amino acid with the microelements in proportion. The invention also provides a preparation method and an application of the fertilizer. Agricultural resources are used as the main raw materials to prepare a new generation of the environmentally friendly amino acid fertilizer by biological fermentation technology, and the fertilizer is applied to the plants, with good plant absorption efficiency. The fertilizer takes effect quickly and has obvious effects of initiating roots, promoting seedlings, strengthening rods, increasing the resistance ability, preventing cracking and increasing the yield and the quality; and moreover the fertilizer is safely applied, and widely used in green organic agriculture and agricultural facilities.
Description
Technical field
The invention belongs to technical field of agricultural fertilizers, particularly a kind of active amino acid water-soluble fertilizer and its preparation method and application.
Background technology
Along with improving constantly of human living standard, people are also more and more higher to the specification of quality of farm crop and agricultural byproducts thereof, only can not meet the requirement of green organic food supply and production by traditional planting patterns and long-term application chemical fertilizer, so the exploitation of vegetable protein resource are the emphasis of producing the development of resources of the water-soluble fertilizer of amino acid.China is the native place of soybean, aboundresources, so soya products exploitation, soybean further process is by the most important thing that is the systems engineering of amino acid water-soluble fertilizer.
In agriculture production, there is the amino acids material of multiple biocatalysis in application at present.Nearest research has also proved that Lalgine, amino acid, silicic acid, Whitfield's ointment, propiconazole, trinexapac-ethyll, Bian Ji purine etc. are very promising.These all materials produce useful effect by hormone effect, raising antioxidant levels and raising resistance.
Amino acid is the basic comprising unit of protein and enzyme.Its synthetic and absorption is the key of cells survival.The amino acid overwhelming majority on agricultural materials market derives from the degradation production of protein: amino acid and peptide.The hydrolysis of protein has following approach: chemical process decomposition, enzyme decomposition, acetic acid anaerobic digestion, microwave decomposition etc.First the method for the animal source protein hydrolyzate obtaining by chemical treatment is applied in agricultural, and its product is also the agricultural amino acid main flow raw material on current global agricultural materials market.According to the experience of main flow producer (Italian SICIT company), chemical hydrolysis allows the accurate control to proteolysis degree and free aminoacid content.These ideas have been identified and have applied as controlling factor in the biostimulation character of protein hydrolyzate.
Enzyme is to have biologically active substance, can specifically act on peptide compound, namely forms on the chain between the amino acid of protein.Introduce according to obtain amino acid whose manufacturers (Hispanic Bioberica) by enzymolysis approach, the Main Differences that obtains a kind of amino acid whose method and acidolysis and alkaline hydrolysis method by enzymolysis protein matter is: (1) does not have amino acid degradation, some amino acid as tryptophane and Gelucystine be to be very easy to degrade under extreme acid-basicity or hot conditions.Under mild condition, do the used time when enzyme, all kinds amino acid that final product comprises different quantities; (2) can there is not the chemical transformation of L-type to D type in amino acid; (3) high de-agglomeration rate: any protein raw material all can obtain high total free aminoacids ratio by mode of action.
Nineteen sixty-eight, in Italy, first amino acid and peptide are introduced into and test in agricultural.At present, gondola first commercial prod is all being sold in locality or transnational company all over the world.Now, many based on amino acid whose product as a kind of " pure natural organic fertilizer " production and selling, but be all to do under the name of biocatalysis.Simultaneously in West Europe, North America, much different research is being carried out, but Eastern Europe is more more.Not same-action and the mechanism of action of these amino acid and peptide pointed out in these researchs.They are playing the part of an important role research discovery in Physiological Activities of Plants.Therefore, they stimulate affects mechanism and regulates nutrition to transform the activity influence of the enzyme of metabolic process to some plant-growth.
Scientific research shows crop to blade spraying or fills with the amino acid used of root have very high absorption, transport and utilize ability.On agricultural materials market, there are many amino acid products as biological catalyst, appropriate use, some is output, quality and the resistance that can improve agricultural-food, and some is can not.So being exactly the ratio based on amino acid kind, concentration and some specific amino acids and peptide and the mechanism that affects plant-growth, the present invention studies.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of active amino acid water-soluble fertilizer.Another object of the present invention is to provide the preparation method of above-mentioned active amino acid water-soluble fertilizer.
In order to realize the object of the invention, a kind of active amino acid water-soluble fertilizer provided by the invention, it is liquid or solid shaped dosage forms, water-soluble, the component that contains following weight part:
Preferably, the component that fertilizer of the present invention contains following weight part:
More preferably, the component that fertilizer of the present invention contains following weight part:
Wherein, described active mixed amino acid, its effective constituent is for having bioactive L-amino acid and bioactive peptide, by high-temperature denatured dregs of beans → pulverized 120 mesh sieve samples → by with add water adjusting → adjusting ph value 6.5-7.5 → add composite fungus agent → consider except residue filter → active amino acid supplement by pressure filter be hydrolyzed → enzymolysis solution → supernatant liquor of NaOH solution adjust pH 6.5-7.5 of 2mol/L of the weight ratio 1:8-9 of water.
Particularly, active mixed amino acid prepares by the following method:
1) agricultural byproducts are crossed 120 mesh sieves, by weight substrate: the ratio that water is 1:8-9 adds water, and described agricultural byproducts are high-temperature denatured dregs of beans or any proteinaceous agriculture organism and agricultural byproducts;
2) prepare composite fungus agent: aspergillus oryzae (Aspergillus oryzae), trichoderma harziarum (harzianum), subtilis (Bacillus subtilis) are mixed and made into composite fungus agent by the weight ratio of 1.5-2.5:0.5-1.5:0.5-1.5;
3) composite fungus agent is added in the agricultural byproducts that step 1) obtains, adopt enzyme hydrolysis method that agricultural byproducts enzymolysis is obtained to active mixed amino acid.
Preferably, above-mentioned three kinds of equal weight proportions are 2:1:1.
Step 2) in the viable count of aspergillus oryzae (Aspergillus oryzae) be 0.5 × 10
9-1.5 × 10
9cfu/g, the viable count of trichoderma harziarum (harzianum) is 0.2 × 10
9-0.8 × 10
9cfu/g, the viable count of subtilis (Bacillus subtilis) is 0.2 × 10
9-0.8 × 10
9cfu/g.
In described step 3), composite fungus agent is as the precursor of compound protease, after adding in the mixture of water and agricultural byproducts, the effect of performance compound protease, therefore composite fungus agent add after the specific activity of enzyme of its generation should be 16000-20000u/g.Enzyme activity unit definition: under 25 ℃ of conditions, reaction 1min discharges the needed enzyme amount of 1nmol product.U represents the unit of activity of enzyme.U/g is specific activity, every gram of enzyme activity that zymoprotein has, and unit is u/g, the higher enzyme of specific activity is purer.
The hydrolysis temperature of step 3) is 50-60 ℃, and enzymolysis time is 2-4h, and pH value is 6.5-7.5.
In step 3), in the time that being 31.8-41.7%, protein degree stops hydrolysis.
Described chelated trace element of compound amino acid is that above-mentioned active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the ratio chelating of weight ratio 2-3:0.5-1.5:0.5-1.5;
Preferably, described chelated trace element of compound amino acid is that above-mentioned active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the ratio chelating of weight ratio 2.5:1:1.
Described chelated trace element of compound amino acid is to measure respectively according to the above ratio after active aminoacids complex mixes with above-mentioned inorganic microelement to react, and whole anti-temperature is carried out at 55-65 ℃, and time 0.5-1.0 hour controls pH value 6.5-7.5;
Described plant nutrient is urea;
Described plant promoting agent is sodium nitrophenolate 0.1-0.3g/L, and a-Nafusaku 0.03-0.07g/L is formulated by the two volume ratio 3-5:0.5-1.5.
The preparation method of described active amino acid water-soluble fertilizer, stirs active amino acid supplement, chelated trace element of compound amino acid, plant nutrient and plant promoting agent fully to mix and be processed into by aforementioned ratio.Be about to according to active mixed amino acid 480-836 part of weight part meter; Chelated trace element of compound amino acid 210-410 part; Plant nutrient 8-16 part; Plant promoting agent 14-26 part mixing and stirring prepares.
Preferably, it is by according to active mixed amino acid 510-806 part of weight part meter; Chelated trace element of compound amino acid 260-360 part; Plant nutrient 9-15 part; Plant promoting agent 16-24 part mixing and stirring prepares.
More preferably, it is by according to 658 parts of the active mixed amino acids of weight part meter; 310 parts of chelated trace element of compound amino acid; 12 parts of plant nutrients; 20 parts of mixing and stirring of plant promoting agent prepare.
Described active amino acid water-soluble fertilizer is liquid or powdery or particulate state formulation.
Active mixed amino acid is to utilize agricultural byproducts resource for main raw material, the high-tech of new generation of preparing by biofermentation technique, multi-functional, environment-friendly type amino-acid liquid; The main raw material of described agricultural byproducts resource is high-temperature denatured dregs of beans or any proteinaceous agriculture organism and agricultural byproducts thereof.
Described biofermentation technique is that composite protease hydrolysis method is enzyme process.Enzyme process reaction temperature and, facility investment is few, production process is corrosion-free and pollute, and does not produce harmful side product, quality product is best, is the most advanced method of current protein hydrolysate.Zymyhydrolyzed protein matter is by considering degree of hydrolysis and production cost, to reach the requirement of low-cost high-efficiency.
The present invention also provides the application of above-mentioned active amino acid water-soluble fertilizer in agriculture production.Product of the present invention is according to absorption and the conversion capability of fertilizer requirement for crop growth rule and leaves and stems nutrient, carries out nutrient supply and transformation function conditioning and a kind of broad spectrum type amino acid water-soluble fertilizer of development.Adopt up-to-date biological enzymolysis technique, tens kinds of nutrition elite that biopolymerization crop is required, be rich in bioactive peptide, L-amino acid, also include nitre state, ammonium state, amidonitrogen simultaneously, rapid available phosphorus, potassium and chelated ferrous iron, manganese, zinc, copper, boron, xanthohumic acid, probiotics, plant regulation agent etc., between each nutrient, compatible property is good, and easy utilization absorbed by crops has antibacterial disease-resistant, the effect that improves plant materials immunologic function, regulating crop growth.The raw material that the present invention adopts is easy to get, and preparation and application is simple, operation is easily gone.
The advantage of product of the present invention is:
1, product utilization agricultural byproducts resource of the present invention is main raw material, the high-tech of new generation of producing by biofermentation technique, multi-functional, environment-friendly type active amino acid liquid fertilizer.It is rich in bioactive peptide, L-amino acid, calcium constituent, spontaneous growth active substance.There is the effects such as significant root of hair, short seedling, strong bar, degeneration-resistant, anticracking, volume increase, high-quality, can be widely used in industrialized agriculture and green agriculture.
2, product of the present invention is multiple agricultural-food fermentative degradation material, and active amino acid content is high, Crop excellent in efficiency, and instant effect, and use safety, be a kind of environmental friendly fertilizer.
3, product of the present invention there is hestening rooting, bloom, bear fruit, anti-fruit-cracking, the effect of hyperchromic pool, and have good resistance (freeze injury, drought injury, wet injury, disease etc.).Especially can make the plant being injured recover rapidly growth.
4, product of the present invention can promote melon, really, vegetables maturation, go on the market ahead of time about one week, and can extend about one month collection period, make crop yield 10%-0%.Obviously improve quality of agricultural product, be conducive to fresh-keeping, storage simultaneously.
5, product of the present invention adds in the soil body by adding composite fungus agent, make that Soil Microorganism fauna distributes, the environment of an activation is improved, the generation of increased activity and a large amount of derivatives, chelating or the direct harm of obstruct heavy metal to crop, have the function that rehabilitating soil pollutes.
6, product of the present invention is exerted into be combined with mineral colloid in the soil body by punching and forms organic-inorganic glue complex body, has the soil of improvement reason, voltinism energy, strengthens soil water-retaining, fertilizer-preserving ability.
7, bioactive peptide, the L-amino acid etc. in product of the present invention has stable crop cell potential of hydrogen and improves ability resistant to lodging.
8, active amino acid water-soluble fertilizer of the present invention is liquid or solid shaped dosage forms, and application process is simple and practical, can spray at crop over-ground part, can be used as again rushing spraying fertilizer.
9, the field effect of active amino acid water-soluble fertilizer of the present invention is good.
Three bacterial classification sources that the present invention relates to are as follows respectively:
1, aspergillus oryzae (Aspergillus oryzae) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC30155;
2, trichoderma harziarum (harzianum) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC30371;
3, subtilis (Bacillus subtilis) China Committee for Culture Collection of Microorganisms agricultural microorganism center SCTCC1132.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.Without departing from the spirit and substance of the case in the present invention, the modification that the inventive method, step or condition are done or replacement, all belong to protection scope of the present invention.
If do not specialize, the conventional means that in embodiment, technique means used is well known to those skilled in the art.If do not specialize, in embodiment, chemical reagent used is commercially available.
The preparation (1) of embodiment 1 active amino acid water-soluble fertilizer
1, composite fungus agent formula
The effective constituent of its precursor of compound protease is the composite fungus agent that aspergillus oryzae, trichoderma harziarum, subtilis are mixed by the part by weight of 2:1:1; In microbial inoculum, the viable count of aspergillus oryzae is 1.0 × 10
9cfu/g, the viable count of trichoderma harziarum is 0.5 × 10
9cfu/g, the viable count of subtilis is 0.5 × 10
9cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, PORT OF LANSHAN, Rizhao of Shandong Province), for main raw material, is that composite protease hydrolysis method is carried out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:9 with water, add composite fungus agent, requiring the addition of control enzyme (being the composite fungus agent of the present embodiment) is 18000u/g; Hydrolysis temperature is controlled at 55 ℃; Enzymolysis time is decided to be 4h; Regulation and control ph value is 7.0; In the time that protein degree reaches 36.8% in high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except residue clarification by pressure, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
The active mixed amino acid making in step 2, borax, Zinc Sulphate Heptahydrate are formed according to the part by weight chelating of 2.5:1:1; Its inner complex is to measure respectively by weight proportion active aminoacids complex to react with above-mentioned inorganic microelement, 60 ℃ of whole reaction constant temperature, and time 0.5h, controls pH value 7.0.
4, the preparation of active amino acid water-soluble fertilizer
By according to 658 parts of the active mixed amino acids of weight part meter; 310 parts of chelated trace element of compound amino acid; 12 parts, plant nutrient urea; 20 parts of (the two volume ratio is 3:0.5 for sodium nitrophenolate 0.2g/L, a-Nafusaku 0.04g/L) mixing and stirring of plant promoting agent prepare.
The preparation (2) of embodiment 2 active amino acid water-soluble fertilizers
1, composite fungus agent formula
The effective constituent of its precursor of compound protease is the composite fungus agent that aspergillus oryzae, trichoderma harziarum, subtilis are mixed by the part by weight of 2:1.5:1.5; In microbial inoculum, the viable count of aspergillus oryzae is 0.8 × 10
9cfu/g, the viable count of trichoderma harziarum is 0.6 × 10
9cfu/g, the viable count of subtilis is 0.6 × 10
9cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, PORT OF LANSHAN, Rizhao of Shandong Province), for main raw material, is that composite protease hydrolysis method is carried out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8 with water, add composite fungus agent, requiring the addition of control enzyme (being the composite fungus agent of the present embodiment) is 20000u/g; Hydrolysis temperature is controlled at 50 ℃; Enzymolysis time is decided to be 3h; Regulation and control ph value is 7.5; In the time that protein degree reaches 41.7% in high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except residue clarification by pressure, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 3:1.5:1.5; Its inner complex is to measure respectively by weight proportion active aminoacids complex to react with above-mentioned inorganic microelement, and whole temperature of reaction regulates and controls at 55 ℃, and time 1.0h controls ph value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
By according to 580 parts of the active mixed amino acids of weight part meter; 388 parts of chelated trace element of compound amino acid; 9 parts, plant nutrient urea; 23 parts of (the two volume ratio is 5:0.5 for sodium nitrophenolate 0.1g/L, a-Nafusaku 0.03g/L) mixing and stirring of plant promoting agent prepare.
The preparation (3) of embodiment 3 active amino acid water-soluble fertilizers
1, composite fungus agent formula
The effective constituent of its precursor of compound protease is the composite fungus agent that aspergillus oryzae, trichoderma harziarum, subtilis are mixed by the part by weight of 1.5:1.5:1.4; In microbial inoculum, the viable count of aspergillus oryzae is 0.5 × 10
9cfu/g, the viable count of trichoderma harziarum is 0.8 × 10
9cfu/g, the viable count of subtilis is 0.7 × 10
9cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, PORT OF LANSHAN, Rizhao of Shandong Province), for main raw material, is that composite protease hydrolysis method is carried out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8.6 with water, add composite fungus agent, require to control the addition 19000u/g of enzyme (being the composite fungus agent of the present embodiment); Hydrolysis temperature is controlled at 60 ℃; Enzymolysis time is decided to be 2h; Regulation and control ph value is 6.5; In the time that protein degree reaches 39.3% in high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except residue clarification by pressure, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 3:1:1; Its inner complex is to measure respectively by weight proportion active aminoacids complex to react with above-mentioned inorganic microelement, and whole temperature of reaction regulates and controls at 60 ℃, and time 1.0h controls pH value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
By according to 680 parts of the active mixed amino acids of weight part meter; 288 parts of chelated trace element of compound amino acid; 11 parts, plant nutrient urea; 21 parts of (sodium nitrophenolate 0.3g/L, a-Nafusaku 0.07g/L, the two volume ratio 4:1.5) mixing and stirring of plant promoting agent prepare.
The preparation (4) of embodiment 4 active amino acid water-soluble fertilizers
1, composite fungus agent formula
The effective constituent of its precursor of compound protease is the composite fungus agent that aspergillus oryzae, trichoderma harziarum, subtilis are mixed by the part by weight of 2.5:1:1; In microbial inoculum, the viable count of aspergillus oryzae is 1.5 × 10
9cfu/g, the viable count of trichoderma harziarum is 0.25 × 10
9cfu/g, the viable count of subtilis is 0.25 × 10
9cfu/g.
2, the preparation of active amino acid supplement
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, PORT OF LANSHAN, Rizhao of Shandong Province), for main raw material, is that composite protease hydrolysis method is carried out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8.9 with water, add composite fungus agent, require to control the addition 17000u/g of enzyme (being the composite fungus agent of the present embodiment); Hydrolysis temperature is controlled at 55 ℃; Enzymolysis time is decided to be 2.5h; Regulation and control ph value is 7.0; In the time that protein degree reaches 38.6% in high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except residue clarification by pressure, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 2:1.5:1.5; Its inner complex is to measure respectively by weight proportion active aminoacids complex to react with above-mentioned inorganic microelement, and whole temperature of reaction regulates and controls at 65 ℃, and time 0.5h controls ph value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
By according to 758 parts of the active mixed amino acids of weight part meter; 210 parts of chelated trace element of compound amino acid; 14 parts of plant nutrients; 18 parts of (sodium nitrophenolate 0.25g/L, a-Nafusaku 0.05g/L, the two volume ratio 4:1) mixing and stirring of plant promoting agent prepare.
The manure trial of embodiment 5 in romaine lettuce plantation
By regular field test, the active amino acid water soluble fertilizer that uses embodiment 1 to make, observes its fertilizer efficiency on romaine lettuce, to evaluate this fertile effect of increasing production and economic benefit, for demonstration, the popularization of this fertilizer provide scientific basis.
1 materials and methods
1.1 test site
Test (in booth) in the vegetable fields of Agro-ecology Technology Park, Gu Gang town, Liuyang City is carried out, and soil fertility is better-than-average, and irrigation and drainage are convenient.
1.2 test materials
For studying thing: balling romaine lettuce, purchased from means of agricultural production service department of academy of agricultural sciences of Hunan Province.
For trying fertilizer: the amino acid water soluble fertilizer that the embodiment of the present invention 1 makes, this fertilizer is liquid, free aminoacid content>=100g/L, micronutrient levels>=20g/L, the non-water solubles≤50g/L, pH value 3.0-7.0,6.5 yuan/kg of market price; Blue Chemical Mixed Fertilizer (the N: P: K of Norway
2o=16: 0: 14), 3500 yuan/t of market price; Yuntianhua is produced urea (N>=46%), 2500 yuan/t of market price.
1.3 tests are processed
Process 1: each per hectare is watered and executed reduced urea 20% in the fertilizer 150kg+ conventional fertilizer application that embodiment 1 makes; Process 2: each per hectare is watered and executed reduced urea 20% in embodiment 1 fertilizer 225kg+ conventional fertilizer application; Process 3: conventional fertilizer application; Process 4: water equivalent clear water (CK)+conventional fertilizer application decrement 20%.
1.4 test design
Test adopts random district group to arrange, and processes 3 times for 4 and repeats.Zone leader 8m, wide 1.5m, minizone is every 0.5m, community area 12m
2, every community plants 20 row, and every row is planted 4 strains, totally 80 strains, plantation specification is line-spacing 37.5cm × spacing in the rows 40cm, per hectare 66705 strains.On October 4th, 2010 transplants, and transplanted seedling is that the dish that floating seedlings is bred as is grown seedlings, and adopts the row bars such as individual plant to plant when seedling 2 leaf 1 heart.Fertilization time and quantity refer to table 1.000 times of Pentium 4, good field liquid spraying for each processing on October 14, October 29 is each processes with 500 times of liquid of phytokinin+must 500 times of liquid of good fortune+1500 times of liquid spraying insects of Qingling go out, sprayed the de-3000 times of liquid of enemy's power 1 time on October 24, sprayed the de-1500 times of liquid of 1500 times of liquid+agricultural streptomycins of 3000 times of liquid+triazolones of enemy's power 1 time on October 26, November 14 gathered in the crops.Fertilising quantity and time are in Table 1-1.
2 results and analysis
2.1 different treatment Analysis of agronomic characters
The amino acid water soluble fertilizer different treatment that embodiment 1 makes on the impact of romaine lettuce Other Main Agronomic Characters in Table 1-2.From table 1-2, to process 1,2 and compare with processing 3,4, it is strong that growing period shows as plant, and growing way is strong, and color is dark green, and color and luster is good, and commodity property is good.When results, through investigation, process 1,2 leaf-head than processing 3,4 difference high 0.6cm, 0.8cm.The horizontal stem processing 2 of leaf-head is the widest, respectively than processing 1, process 3, processing 4 wide 0.5cm, 0.3cm, 0.7cm.The single leaf-head net weight processing 2 of individual plant is the highest, is secondly to process 1; Process 2 than processing 1, processing 3, processing 4 high 0.01kg, 0.05kg, 0.07kg respectively; Clean vegetables rate is also that processing 2 is the highest.
2.2 different treatment volume analyses
Different treatment yield result is in Table 1-3.From table 1-3, process 2 output the highest, output per hectare 56667.0kg, than processing 1, processing 3, processing 4 high 833.4kg, 2750.1kg, 4750.1kg respectively, output occupies the 1st; Process 1 output and take second place, per hectare is produced 55833.6kg, and than processing 3, processing 4 difference high 1916.7kg, 3916.7kg, output occupies the 2nd.
2.3 variance analysis
The results of analysis of variance (table 1-4) shows that F=13.057 > F0.01=9.73 between processing exists utmost point significant difference between showing to process.Carry out multiple comparisons (table 1-5) with the poor SSR of new multipole, result shows, process 2 with process 3, process 4 and have utmost point significant difference, and process 1 indifference; Processing 1 has utmost point significant difference with processing 4, there is no significant difference with processing 3; Indifference between processing 3 and processing 4.
2.4 different treatment Economic and Efficiency Analysis
The amino acid water soluble fertilizer of using embodiment 1 on romaine lettuce has good economic benefit, the highest to process 2 economic benefits, per hectare is than processing 4(CK) net output is high 10420.05 yuan, than processing 3(routine) net output is high 5600.25 yuan, higher 1596.0 yuan than processing 1;
Table 1-1 amino acid water-soluble fertilizer fertilization time, quantity
The Other Main Agronomic Characters of table 1-2 different treatment
The volume analysis of table 1-3 different treatment
Table 1-4 variance analysis
| Source of variation | DF | SS | MS | F | F0.05 | F0.01 |
| Process | 3 | 57.667 | 19.222 | 13057 | 4.757 | 9.78 |
| District's group | 2 | 18.500 | 19.22 | 6.283 | 5.143 | 10.925 |
| Error | 6 | 8.833 | 1.472 | ? | ? | ? |
| Total variation | 11 | 85.000 | ? | ? | ? | ? |
The poor comparison of the table new multipole of 1-5
The Economic and Efficiency Analysis of table 1-6 different treatment
Note: romaine lettuce price is calculated by 2.5 yuan/kg of market price.
Next is to process 1, and per hectare net output is than processing 4(CK) high 8824.05 yuan, than processing 3(routine) high 4004.25 yuan.
3 discuss
It is extremely remarkable that the amino acid water soluble fertilizer that the embodiment of the present invention 1 makes is used effect of increasing production on Liuyang City romaine lettuce crop, and there is the advantages such as easy to use, safe, environmental protection, this fertilizer of long-term application can improve soil organic matter content, improve soil physical and chemical property, can on Liuyang City's romaine lettuce is produced, promote the use of.Application process: per hectare is watered at every turn and executed reduced urea 20% in amino acid water soluble fertilizer 225kg+ conventional fertilizer application of the present invention.
The manure trial of embodiment 6 on tomato planting
Foliage fertilizer can supplement crop alimentary, and regulating crop growth, for the practical application effect of checking amino acid water-soluble fertilizer of the present invention on tomato, is subject to the trust at clay fertilizer station, Hubei Province, has carried out application test on the tomato of Shashi.
1 materials and methods
1.1 test materials
Be the active amino acid water-soluble fertilizer that embodiment 2 makes for examination fertilizer, Tai Gu biotech inc, Hunan produces, amino acid >=100g/L, copper+zinc+manganese+iron >=20g/L, and product forms is aqua; Educate No. 3 tomatoes of space for studying thing for boat, provided by Anhui Lu'an City gold soil Agricultural Popular Science Demonstration Garden.This kind plant height 80-100cm, 6-7 sheet leaf raw 1 inflorescence, is medium variety morning, strong stress resistance, growth potential is prosperous, fruit is high circular, pink, taste sweet not sour, quality is good, pulp is thick, storage tolerance and transportation; General single fruit weight 200g left and right, maximum reached at 500g, fruit size is even, neat appearance, 667m
2output can reach 5000-7500kg.
1.2 test method
Test is located at Shashi agricultural experiment station, is silty loam for examination soil, and soil fertility is medium.Before test, soil sampling is measured, organic content 24.58g/kg, full nitrogen 1.35g/kg, available phosphorus 25.9g/kg, available potassium 96.5g/kg, pH value 7.7.
1.3 test design
3 processing are established in test, 1. process 1: base manure, topdressing uses by local conventional, does not use foliage fertilizer; 2. process 2: each all with the equivalent clear water with processing 3, at the same time between foliage-spray, other measures are with processing 1; 3. process 3: every 667m
2with being watered 80kg foliage-spray containing amino acid water-soluble fertilizer 150mL, respectively at spray in seedling stage (before bud differentiation) 1 time, 2 times (interval 8d) of fruit expanding period spray, other measures are with processing 1 at every turn.Test adopts randomized block design, repeats totally 9 communities, community area 20m 3 times
2.Tomato was sowed on November 21st, 2009, field planting on February 2 in 2010, every 667m
2plant 2600 strains, be 14 days-July 20 May in 2010 harvesting time.Every 667m
2execute well-rotted farmyard manure 3500kg, efficient compound fertilizer 50kg makes base manure; In the 1st fringe fruit stone peach when size, is in conjunction with watering every 667m
2impose urea 6.5kg, the 2nd, 3 fringe fruits expanding stage rapidly, each every 667m
2impose urea 7.5kg, topdress altogether 3 times.Adopt single stalk training, every fringe stays 3-4 of fruit.Except spraying foliage fertilizer, each rich water of processing is planned strategies for, the prevention and control of plant diseases, pest control, agronomic measures are all consistent.The Foliar Spraying time is January 27, March 15,23 days.
2 results and analysis
2.1 impacts on output
From table 2-1, amino acid water-soluble fertilizer (the processing 3) mean yield that embodiment 2 makes is 134.28kg, with the check plot of conventional fertilizer application (processing 1) and spray compared with the check plot (processing 2) of equivalent clear water, tomato yield increases respectively 9.62kg and 9.13kg, amounts to 667m
2output increases respectively 320.83kg and 304.49kg, and stimulation ratio is respectively 7.717% and 7.295%.For the accuracy of check test result, data have been carried out to variance analysis, between processing, volume variance reaches utmost point conspicuous level, and repeat between without significant difference (in Table 2-2).
LSD test result shows, in the situation that Routine Management is consistent, on tomato, sprays clear water and do not spray and there is no significant difference, but containing amino acid water-soluble fertilizer with spray, do not spray contrasting of clear water and all reached effect of increasing production (in Table 2-3) extremely significantly.
Table 2-1 tomato sprays the impact of foliage fertilizer on output
Table 2-2 tomato sprays the yield result analysis of variance table of foliage fertilizer test
| Source of variation | Degree of freedom | Sum of squares | Root mean square value | F value | Significant difference |
| Between repetition | 2 | 0.285 | 0.14 | 0.11 | ? |
| Between processing | 2 | 176.14 | 88.07 | 65.85 | ** |
| Testing error | 4 | 5.35 | 1.34 | ? | ? |
| Total variation | 8 | 181.78 | ? | ? | ? |
Table 2-3 tomato sprays the multiple comparisons of the yield result of foliage fertilizer test
2.2 impacts on tomato single fruit weight
From table 2-4, amino acid water-soluble fertilizer (processing 3) the tomato mean fruit weight of embodiment 2 is 235.80g, with conventional fertilizer application (process 1) and spray compared with equivalent clear water (processing 2), tomato single fruit weight has increased respectively 15.52g and 15.29g, and rate of body weight gain is respectively 7.05% and 6.93%.
Table 2-4 tomato sprays the mean fruit weight result table of foliage fertilizer test
For the accuracy of check test result, data have been carried out to variance analysis, between processing, volume variance reaches utmost point conspicuous level (F=57.04** between processing), and repeat between without significant difference (in Table 2-5).
Table 2-5 tomato sprays the yield result analysis of variance table of foliage fertilizer test
| Source of variation | Degree of freedom | Sum of squares root mean square value | F value | Significant difference |
| Between repetition | 2 | 0.880.44 | 0.11 | ? |
| Between processing | 2 | 474.91237.46 | 57.04 | ** |
| Testing error | 4 | 16.654.16 | ? | ? |
| Total variation | 8 | 492.45 | ? | ? |
LSD test result shows, in the situation that Routine Management is consistent, tomato single fruit weight is with spray clear water and do not spray clear water and there is no significant difference, but tomato spray and the contrast ratio that does not spray clear water after spraying containing amino acid water-soluble fertilizer, its single fruit gaining effect has all reached extremely significant level (in Table 2-6).
Table 2-6 tomato sprays the multiple comparisons of the yield result of foliage fertilizer test
2.3 impacts on economic benefit
By 2.5 yuan of calculating of the average every kg price of tomato, the producible economic benefit of different treatment is in Table 2-7.
Table 2-7 Economic and Efficiency Analysis table
From table 2-7, on the basis of conventional fertilizer application, tomato sprays containing amino acid water-soluble fertilizer obvious the effect of increasing income, process 3 with contrast 1,2 and compare, every 667m
2new output value is respectively 802.08 yuan, 761.23 yuan, 6 yuan of deduction foliage fertilizer costs, every 667m
2have a net increase of 796.08 yuan of benefits, 755.23 yuan, input-output ratio reaches 1: 133 and 1: 126, and economic benefit is considerable.
3 conclusions
Under this test conditions, at spray in the seedling stage of tomato (before bud differentiation) 1 time, 2 times (interval 8d) of fruit expanding period spray, the amino acid water-soluble fertilizer that embodiment 2 makes, each every 667m
2consumption 150mL is watered 80kg, can significantly increase single fruit weight and the output of tomato, and difference reaches utmost point conspicuous level.Contrast with routine and only spray equivalent clear water and compare, amount to 667m
2output has increased respectively 320.83kg and 304.49kg, and stimulation ratio is respectively 7.717% and 7.295%, every 667m
2new output value is respectively 802.08 yuan, 761.23 yuan, 6 yuan of deduction foliage fertilizer costs, every 667m
2have a net increase of 796.08 yuan of benefits, 755.23 yuan, input-output ratio reaches 1: 133 and 1: 126.Visible, the amino acid water-soluble fertilizer of using embodiment 2 on tomato has the effect of good increase output and increase benefit.
The manure trial of embodiment 7 on green vegetables
Foliage application is one of important means of regulating crop growth.Foliage application can supplement the leafy vegetable required nutrient of growing fast, promotes leaf vegetables growth.For verifying the actual effect of amino acid water-soluble fertilizer of the present invention large-scale demonstration application on green vegetables, be chosen in July, 2009 on green vegetables and carried out experiment and demonstration, now will test retribution road as follows.
1 materials and methods
1.1 are located in leaf vegetables base, Katyuan community, Changsha Kaifu District for examination ground experimental tests, 1.85 mu of testing ground areas.
1.2 is silty loam for examination soil testing field soil, soil organic matter content 26.6g/kg, alkali-hydrolyzable nitrogen 101mg/kg, rapid available phosphorus 19.5mg/kg, available potassium 99mg/kg, and soil fertility level is more even.
The 1.3 amino acid water-soluble fertilizers that make for examination fertilizer embodiment 3, amino acid >=100g/L, trace element >=20g/L, product forms is pulvis.
1.4 for studying the little green vegetables of thing, and kind is " green excellent No. 1 ", belongs to heat resistant variety, first-filial generation, and plant type is upright, and petiole is wide flat, and leaf is thick, and light green white is applicable to sowing in 5～September.
1.5 tests are processed and are established 3 processing with design experiment: the 1. conventional (CK that contrasts
1), base topdresses and is undertaken by the conventional service condition in locality, does not use foliage fertilizer and conditioning agent.2. clear water contrast (CK
2), every mu at every turn to carry out foliage-spray with processing the 3. equivalent clear water contemporaneously, and other measures of fertilizer are with processing 1..3. containing amino acid water-soluble fertilizer.Every mu at every turn be watered 800 times of liquid of 40kg(dilution containing amino acid water-soluble fertilizer 50g) carry out foliage-spray, after emerging, 15d sprays 1 time, sprays 1 time every 7d again, sprays altogether the time of infertility 2 times, and other measures of fertilizer is with processing 1..Every processing repeats 3 times, and random district group is arranged, and same district group is arranged in same furrow, community area 36m
2, long-width ratio is 2.5: 1, each minizone isolates with ditch.
Decomposed manure 150kg is executed in 1.6 cultivation management test field, experimental plots, spread fertilizer over the fields 45% compound fertilizer of high concentration (15: 15: 15) 20kg, be July 8 sowing time, process and 3. sprayed containing amino acid water-soluble fertilizer for the 1st time July 26, August 2 sprayed containing amino acid water-soluble fertilizer for the 2nd time, processed and 2. sprayed equivalent clear water simultaneously.
2 results and analysis
2.1 can find out by showing 3-1 the impact of plant trait, process and 3. spray containing after amino acid water-soluble fertilizer, can promote preferably little green vegetables growth, and plant height is than processing 2. (clear water contrast), processing 1. (conventional contrast) high by 2.6,3.2cm respectively; Hundred strain dry weights are 9.5g, 2., 1. weigh respectively 2.2,2.6g than processing, and illustrates to spray containing little green vegetables endotrophic material after amino acid water-soluble fertilizer manyly, have strengthened the ability that high temperature resistance, heat resistanceheat resistant are shone, and resistance enhancing, so growing way is very prosperous.
Table 3-1 plant trait is investigated result
2.2 singly pull out single receipts to the little green vegetables that affect 11Dui Mei in August community of yields of vegetables, each cell production (in Table 3-2), and from final real producing, 3. output is the highest to spray processing containing amino acid water-soluble fertilizer, average 667m
2output is 1216kg, compares CK
2(clear water), CK
1(routine) average 667m
2output increases respectively 130,136kg, and statistical analysis, sprays containing the output of amino acid water-soluble fertilizer and reach compared with the control utmost point significant difference.
Table 3-2 respectively processes yield result
2.3 Economic and Efficiency Analysis are from showing 3-3, though spray containing amino acid water-soluble fertilizer cost higher than contrast (CK
1) (spraying 50 yuan of recruitment meters, 5 yuan of fertilizer meters), but every 667m at every turn
2output has increased 136kg, every 667m
2all net return has increased by 406.8 yuan, and economic benefit is very obvious.
Table 3-3 Economic and Efficiency Analysis
3 brief summaries
3.1 promote growth to spray containing after amino acid water-soluble fertilizer, can promote little green vegetables growth, and chlorophyll is synthetic more, blade fertilizer, large, and stem is thick, and nutritious substances accumulation is many, and the abilities such as high temperature resistance, Resistant strengthen, and growing way is vigorous.
The amino acid water-soluble fertilizer effect of increasing production that 3.2 increase output spray embodiment 3 on little green vegetables is obvious, than clear water contrast, conventional contrast every 667m respectively
2volume increase 130,136kg, statistical analysis, effect of increasing production reaches utmost point conspicuous level.
3.3 increasing benefits spray after the amino acid water-soluble fertilizer of embodiment 3, every 667m
2though cost has increased by 110 yuan, contrast (routine) 667m
2output increases 136kg, every 667m
2income increases 406.8 yuan, produces and throws than reaching 4.1: 1.
The manure trial of embodiment 8 on green cucumber
1, materials and methods
1,1 material
Tested in July, 2010 and be arranged in the old virtuous contracted field of Liuyang City's ecological agricultural garden.Great soil group is cinnamon soil, and quality is middle earth, and fertility is better-than-average, and soil fertility is even.Soil plough horizon nutrient is: organic 13.8g/kg, alkali-hydrolyzable nitrogen 101.5mg/kg, rapid available phosphorus (P
2o
5) 14.2mg/kg, available potassium (K
2o) 103.8mg/kg.
For studying thing cucumber, kind is " excellent No. 1 of Tianjin ".
The water-soluble fertilizer of amino acid that embodiment 4 makes, amino acid >=100g/L, Mn+Zn+B >=20g/L.
1,2 methods
3 processing are established in this test, and random district group is arranged, and repeats community area 30m 3 times
2.Process 1: the fertilizer 30mL that conventional fertilizer application+embodiment 4 makes, to water 30kg, respectively sprays 1 time in cucumber at seedling stage, florescence, sprays altogether 2 times;
Process 2: conventional fertilizer application+spray equivalent clear water 1 same period with processing;
Process 3: conventional fertilizer application.Test is carried out on the basis of local conventional fertilizer application.Conventional fertilizer application is: every 667m
2bottom application high-quality fertilizer 4000kg, 14-15-16 compound potassium sulfate fertilizer 80kg, growing period topdresses 4 times, each every 667m
2impose urea 5kg+ composite fertilizer 8kg.
Experimental field cucumber is live April 20, and distance between rows and hills is 60cm × 35cm, every 667m
2retain plant 3170 strains.Respectively at spraying solution or clear water on May 10, May 26, started results in strict accordance with the requirement of testing program on June 2, July 28 harvest home.Results Shi Yi community is single title of unit list receipts to count and produce respectively, and output is the cumulative production of repeatedly gathering to July 28 June 2, carries out field investigation and species test when results simultaneously.Test is except by the spraying solution or clear water of scheme requirements, and other control measures are with general land for growing field crops.
2, results and analysis
2,1 amino acid fertilizer of the present invention becomes the impact of product factor on cucumber
Spray the one-tenth product factor that amino acid fertilizer of the present invention has improved cucumber.
Table 4-1 field investigation and species test cartogram
| Process density (strain/667m 2） | Per plant number (individual) | Single fruit weight (g) |
| 13170 | 10.9 | 186.8 |
| 23170 | 10.5 | 181.2 |
| 33170 | 10.4 | 181.0 |
Note: in table, data are 3 mean numbers of processing 3 repetitions.
From table, 4-1 can find out: process 1 compared with processing 2, processing 3, per plant number increases respectively 0.4,0.5, and single fruit weight increases respectively 5.6g, 5.8g.Illustrate on the basis of conventional fertilizer application, spraying amino acid fertilizer that the embodiment of the present invention 4 makes can increase per plant number and the single fruit weight of cucumber.
2,2 spray the impact of amino acid fertilizer of the present invention on cucumber yield
By table, 4-2 can find out: process 1 and process 2 average every 667m
2volume increase 300kg, stimulation ratio is 5.9%; Process 2 compared with processing 3, every 667m
2volume increase 57.8kg, stimulation ratio is 1.1%.Each processing yield result is carried out to variance analysis (in Table 4-3), and between processing, volume variance reaches conspicuous level.
Table 4-2 yield result cartogram
Table 4-3 analysis of variance table
| Source of variation | Sum of squares | Degree of freedom | Variance | F | F0.05 | F0.01 |
| Between processing | 448.22 | 2 | 224.11 | 11.54* | 6.94 | 18 |
| Between district's group | 7.05 | 2 | 3.52 | 0.18 | 6.94 | 18 |
| Chance error | 77.69 | 4 | 19.42 | ? | ? | ? |
| Total variation | 532.96 | 8 | ? | ? | ? | ? |
Table 4-4 multiple comparisons table
Employing PLSD method is carried out multiple comparisons (in Table 4-4), and between processing 1 and processing 2, processing 3, volume variance all reaches conspicuous level, and between processing 2 and processing 3, volume variance is not remarkable.
3, brief summary
Test-results shows: on the basis of local conventional fertilizer application, the amino acid fertilizer 30mL making with embodiment 4 is watered 30kg, respectively sprays 1 time in cucumber at seedling stage, florescence, spray altogether 2 times, compared with spraying equivalent clear water the same period, increase per plant number and the single fruit weight of cucumber, average every 667m
2volume increase 404.4kg, stimulation ratio is 6.7%, volume variance reaches utmost point conspicuous level.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (7)
1. an active amino acid water-soluble fertilizer, is made up of the component of following weight part:
Wherein, described its effective constituent of active mixed amino acid is for having bioactive L-amino acid, and prepares by following steps:
1) agricultural byproducts are crossed 120 mesh sieves, take agricultural byproducts as substrate, by substrate: the weight ratio that water is 1:8-9 is mixed;
2) prepare composite fungus agent: by aspergillus oryzae (Aspergillus oryzae), trichoderma harziarum (harzianum), subtilis (Bacillus subtilis) by 1.5-2.5:0.5-1.5:0.5-1.5, weight ratio be mixed and made into composite fungus agent;
3) composite fungus agent is added in the agricultural byproducts that step 1) obtains, adopt enzyme hydrolysis method that agricultural byproducts enzymolysis is obtained to active mixed amino acid;
Described chelated trace element of compound amino acid is that active mixed amino acid, borax, Zinc Sulphate Heptahydrate form by the weight ratio chelating of 2-3:0.5-1.5:0.5-1.5;
Described plant nutrient is urea;
Described plant promoting agent is sodium nitrophenolate 0.1-0.3g/L, and a-Nafusaku 0.03-0.07g/L is formulated by the two volume ratio 3-5:0.5-1.5.
2. fertilizer as claimed in claim 1, is characterized in that, is made up of the component of following weight part:
3. fertilizer as claimed in claim 1, is characterized in that, is made up of the component of following weight part:
4. fertilizer as claimed in claim 1, is characterized in that, in active mixed amino acid preparation process, step 2) in the viable count of aspergillus oryzae (Aspergillus oryzae) be 0.5 × 10
9-1.5 × 10
9cfu/g, the viable count of trichoderma harziarum (harzianum) is 0.2 × 10
9-0.8 × 10
9cfu/g, the viable count of subtilis (Bacillus subtilis) is 0.2 × 10
9-0.8 × 10
9cfu/g.
5. fertilizer as claimed in claim 1, is characterized in that, in active mixed amino acid preparation process, the hydrolysis temperature of step 3) is 50-60 ℃, and enzymolysis time is 2-4h, and pH value is 6.5-7.5.
6. fertilizer as claimed in claim 1, is characterized in that, chelatropic reaction temperature is 55-65 ℃, 0.5-1.0h, and pH value is 6.5-7.5.
7. the fertilizer described in claim 1-6 any one, is characterized in that, described fertilizer is liquid or powdery or particulate state formulation.
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| CN110683910A (en) * | 2019-11-11 | 2020-01-14 | 新疆美丽扩科达拉农业科技有限公司 | Biological pesticide fertilizer and preparation method and application thereof |
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