CN103211881A - Application of sorbus sibirica fruit in preparation of drug or health-care product with anti-oxidation effect - Google Patents
Application of sorbus sibirica fruit in preparation of drug or health-care product with anti-oxidation effect Download PDFInfo
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- CN103211881A CN103211881A CN2013101300846A CN201310130084A CN103211881A CN 103211881 A CN103211881 A CN 103211881A CN 2013101300846 A CN2013101300846 A CN 2013101300846A CN 201310130084 A CN201310130084 A CN 201310130084A CN 103211881 A CN103211881 A CN 103211881A
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Abstract
The invention relates to application of a sorbus sibirica fruit in preparation of a drug or a health-care product with anti-oxidation effect. The sorbus sibirica fruit extract is used as raw material which can be independently used or the sorbus sibirica fruit extract and other drugs with the anti-oxidation effect are prepared into the oral drug or health-care product of any dosage form according to the preparation method of the normal preparation. The sorbus sibirica fruit extract has strong anti-lipid peroxidation capacity, high reducing capacity and can be used for effectively removing 0(02). and.OH, and therefore, the sorbus sibirica fruit extract is a good choice for developing novel efficient natural anti-oxidants.
Description
Technical field
The invention belongs to the purposes field of medicine, relate to Siberia Fructus Sorbi Pohuashanensis fruit extract particularly and have the medicine of antioxidation or the application in the health product in preparation.
Background technology
Diet is unhealthy, and the pressure of live and work, environmental pollution etc. can make all that free radical spreads unchecked in the body, thereby cause oxidative phenomena, and oxidation is body health and old and feeble biggest threat, and body antioxidative ability is strong more, and just healthy more, life is also long more.Studies show that more and more antioxidation is pre-anti-aging important step, because free radical or oxidant can decompose cell and tissue, influences metabolic function, and can cause different health problems.If can eliminate too much oxyradical, relate to aging relevant disease and can both prevent for what many free radicals caused.The antioxidant of human body can self synthesize, also can be by food supply, and additional antioxidant helps the motion body to be reduced the generation of free radical or quickens its removing, with the side effect to free radical resisting.Therefore, absorb enough antioxidants, can delay the health catagen speed.
The Siberia Fructus Sorbi Pohuashanensis is Rosaceae (Rosaceae) Fructus Sorbi Pohuashanensis, belongs to dungarunga, only is distributed in upstream, Burqin river in China, likes growing under PiceameyeriRehd. Et Wils. and the fir mixed forest, and its tree-like grace, figure hold directly, and leaf is large-scale winglike compound leaf, is rich in ornamental value.Spring, snow-white flower was completely set fragrance, and the scarlet desire of mellow fruit in autumn is dripped.
The application of Siberia Fructus Sorbi Pohuashanensis fruit extract in medicine and health product at present also do not appear in the newspapers.
Summary of the invention
The present invention is for the development and use of seeking the new and effective Natural antioxidant are purpose, has proposed Siberia Fructus Sorbi Pohuashanensis fruit extract and has had the medicine of antioxidation or the application in the health product in preparation.
The present invention adopts Siberia Fructus Sorbi Pohuashanensis fruit extract as raw material, list is used or is had the drug regimen of antioxidation with other, the preparation method of preparation routinely, be prepared into the medicine with antioxidation or the health product that are fit to oral any dosage form, as oral liquid, tablet, capsule, granule etc.; Or other injection dosage form.
Siberia Fructus Sorbi Pohuashanensis fruit extract can adopt the extracting method of prior art, extracts solvent and can be water or ethanol etc.
The Siberia Fructus Sorbi Pohuashanensis fruit extract that the present invention obtains, by to its reducing power, anti peroxidation of lipid ability, remove ultra-oxygen anion free radical O
Study of indexes such as ability and removing hydroxy radical OH ability show: Siberia Fructus Sorbi Pohuashanensis fruit extract has very strong anti peroxidation of lipid ability, also has reducing power preferably simultaneously, and can effectively remove O
And OH.Therefore, Siberia Fructus Sorbi Pohuashanensis fruit extract oxidation resistance is strong, is good a selection of the development of new Natural antioxidant efficiently.
The specific embodiment
Embodiment 1
(1) preparation of Siberia Fructus Sorbi Pohuashanensis fruit extract: take by weighing Siberia Fructus Sorbi Pohuashanensis fruit 5g, ethanol with 60% soaks 1h at 60-70 ℃, puts into the ultrasonic 30min of ultrasonic cleaner, continuous 2 times, reuse Rotary Evaporators concentrated extracting solution gets Siberia Fructus Sorbi Pohuashanensis fruit extract.
With the Siberia Fructus Sorbi Pohuashanensis fruit extract that obtains with 30% ethanol with its dissolving standardize solution in the 100mL volumetric flask, standby.Be used for following effect experiment.
(2) effect experiment
1, the mensuration of anti peroxidation of lipid ability:
Respectively at the Siberia Fructus Sorbi Pohuashanensis fruit extract sample that adds 1ml10mg/ml lecithin soln (LLS), 1ml0.4mmol/L ferrous sulfate, 1ml variable concentrations in the sample cell successively, mixing, lucifuge is in 37 ℃ of water-bath 60min, add 2ml trichloroacetic acid (TCA)-thiobarbituricacid (TBA)-HCl mixed liquor, 90 ℃～100 ℃ water-bath 15min, cooling with the centrifugal 10min of 3000r/min, is got supernatant and is surveyed absorbance (As) at 535nm rapidly.Blank pipe replaces the 1ml sample with the 1ml distilled water, and the same sample cell of operational approach can record the absorbance (Ac) of blank pipe, replaces 1ml lecithin with the 1ml distilled water in the reference pipe.The positive contrast of ascorbic acid (VC) the results are shown in Table 1.
Suppression ratio (%)=[(Ac-As)/Ac] * 100
In the formula: Ac is the reference substance absorbance of (replacing sample with distilled water);
As is the absorbance when adding variable concentrations Siberia Fructus Sorbi Pohuashanensis fruit extract sample.
Table 1 Siberia Fructus Sorbi Pohuashanensis fruit extract anti peroxidation of lipid ability is measured
Siberia Fructus Sorbi Pohuashanensis fruit extract is to Fe
2+The lecithin liposome peroxidating that causes has the obvious suppression effect, and suppression ratio increases with the increase of sample quality concentration.When sample concentration was 200 μ g/ml, suppression ratio was 30.68%, and when sample concentration was 600 μ g/ml, suppression ratio reached 49.66%; And ascorbic acid is when concentration is 2000 μ g/ml, and suppression ratio reaches 44.22%.As can be seen from Table 1, Siberia Fructus Sorbi Pohuashanensis fruit extract anti peroxidation of lipid ability obviously is better than ascorbic acid.
2, the mensuration of reducing power
The Siberia Fructus Sorbi Pohuashanensis fruit extract solution 2.5ml that gets variable concentrations adds 2.5ml0.2mo1/L phosphate buffer (pH6.6) and 2.5ml l% Hexacyanoferrate potassium solution (K successively in test tube
3Fe (CN)
6), behind 50 ℃ of water bath heat preservation 20min, cooling fast, add the 2.5ml10% trichloroacetic acid again, with the centrifugal 10min of 3000r/min, get supernatant 2.5ml, add the 2.0ml distilled water successively, the liquor ferri trichloridi of 0.5ml0.1%, abundant mixing, leave standstill 10min after, at 700nm place survey light absorption value, the big more expression reducing power of light absorption value strong more (making reference with distilled water) the results are shown in Table 2.
Table 2 Siberia Fructus Sorbi Pohuashanensis fruit extract reducing power is measured
Generally speaking, the reducing power of sample has obviously relevant with antioxidant activity.In the finite concentration scope, Siberia Fructus Sorbi Pohuashanensis fruit extract reducing power increases with the increase of its concentration, though be lower than the reducing power with the concentration ascorbic acid, it also has stable reducing power preferably.
3, remove ultra-oxygen anion free radical
The mensuration of ability:
(1) the autoxidizable mensuration of pyrogallol: the 50mmol/L Tris-HC1 buffer of getting 4.5ml pH8.2,4.2ml distilled water, after in 25 ℃ of waters bath with thermostatic control, being incubated 20min behind the mixing, the 3mmol/L pyrogallol solution 0.3ml(that is added in 25 ℃ of preheatings after the taking-up immediately prepares with 10mmol/LHCl), blank effective 10mmol/L HCl.Measure solution absorbency at the 320nm place every 0.5min, calculate the value added of per minute absorbance in the range of linearity.
(2) sample determination of activity: before adding pyrogallol, add earlier the sample solution 0.9ml of variable concentrations respectively, distilled water 3.3ml, by the method operation of (1), calculate clearance rate then:
Clearance rate (%)=[(△ A
0-△ A)/△ A
0] * 100
In the formula: △ A
0Be pyrogallol autoxidation speed; △ A is the autoxidation speed difference of pyrogallol behind the Fructus Sorbi Pohuashanensis fruit extract sample of adding Siberia, and unit is the value added of absorbance per minute.
Table 3 Siberia Fructus Sorbi Pohuashanensis fruit extract is removed the ultra-oxygen anion free radical ability and is measured
As can be seen from Table 3, in the mass concentration scope of measuring, increase with mass concentration increases Siberia Fructus Sorbi Pohuashanensis fruit extract to the removing ability of ultra-oxygen anion free radical, when sample concentration is 300 μ g/ml, removing ability to ultra-oxygen anion free radical has reached 83.33%, Siberia Fructus Sorbi Pohuashanensis fruit extract has good removing ultra-oxygen anion free radical ability, but is lower than the removing ability of ascorbic acid to ultra-oxygen anion free radical.
4, remove the mensuration of hydroxy radical (OH) ability:
Utilize H
2O
2Produce OH with the Fe2+ reaction, add the salicylic acid seizure and produce coloring matter in system, this material has absorption maximum under 510nm.Contain 8.8mmol/L H in the reaction system
2O
21ml, 10mmol/L FeSO
41ml, 10mmol/L salicylic acid 1ml adds variable concentrations Siberia Fructus Sorbi Pohuashanensis fruit extract solution 1ml respectively.Add H at last
2O
2Start reaction, 37 ℃ of reaction 0.5h make reference with distilled water, survey absorbance under 510nm.Consider the light absorption value of sample itself, with 10mmol/LFeSO
41ml, 10mmol/L salicylic acid 1ml, the Siberia Fructus Sorbi Pohuashanensis fruit extract solution 1ml of variable concentrations and 1ml distilled water are as the background values of sample.The clearance rate computing formula:
Clearance rate (%)=[(A
0-(A
1-A
2))/A
0] * 100
In the formula: A
0Be the absorbance of contrast liquid, A
1Be the absorbance behind the adding sample solution, A
2For not adding developer H
2O
2The absorbance of sample solution.Ascorbic acid is made positive control.
Table 4 Siberia Fructus Sorbi Pohuashanensis fruit extract is removed the hydroxy radical ability and is measured
Adopt salicylic acid method, fixation response time is measured the scavenging action of Siberia Fructus Sorbi Pohuashanensis fruit extract sample to OH.The results are shown in Table 4, in the mass concentration scope of measuring, the increase with mass concentration increases sample to the removing ability of hydroxy radical.When mass concentration is 200 μ g/ml, the clearance rate of hydroxy radical is reached 60.49%; When mass concentration is 400 μ g/ml, the clearance rate of hydroxy radical is reached 99.3%, it is better to remove effect, but is lower than the removing ability of ascorbic acid to hydroxy radical.
In sum, the result shows that Siberia Fructus Sorbi Pohuashanensis fruit extract has very strong anti peroxidation of lipid ability, and (when the sample concentration of Siberia Fructus Sorbi Pohuashanensis fruit extract was 600 μ g/ml, suppression ratio reached 49.66% obviously to be better than ascorbic acid; And ascorbic acid is when concentration is 2000 μ g/ml, and suppression ratio reaches 44.22%); Also have reducing power preferably simultaneously, and can effectively remove
(when sample concentration is 300 μ g/ml, the removing ability of ultra-oxygen anion free radical having been reached 83.33%) and OH(reach 60.49% to the clearance rate of hydroxy radical when sample concentration is 200 μ g/ml).Conclusion: the oxidation resistance of Siberia Fructus Sorbi Pohuashanensis fruit extract is strong, is good a selection of the development of new Natural antioxidant efficiently.
It is ethanol that solvent is extracted in the preparation of present embodiment Siberia Fructus Sorbi Pohuashanensis fruit extract, also can adopt decocting method to obtain extract.
Claims (3)
1. Siberia Fructus Sorbi Pohuashanensis fruit extract has the medicine of antioxidation or the application in the health product in preparation.
2. according to the described application of claim 1, it is characterized in that Siberia Fructus Sorbi Pohuashanensis fruit extract list usefulness or have the antioxidation medicine to have the medicine of antioxidation or the application in the health product in preparation with other.
3. application as claimed in claim 1 or 2 is characterized in that described medicine or health product are peroral dosage form or injection dosage form.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104622998A (en) * | 2015-01-05 | 2015-05-20 | 辽宁大学 | Applications of fructus sorbus sibirica extract in preparation of lipid-lowering medicament |
| CN106177015A (en) * | 2016-08-01 | 2016-12-07 | 吉林大学 | Sorbus domestica application in preparing xanthine oxidase inhibitor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481643A (en) * | 2008-12-25 | 2009-07-15 | 辽宁省干旱地区造林研究所 | Aroniamelanocarpa fruit wine and brewing method thereof |
-
2013
- 2013-04-12 CN CN2013101300846A patent/CN103211881A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481643A (en) * | 2008-12-25 | 2009-07-15 | 辽宁省干旱地区造林研究所 | Aroniamelanocarpa fruit wine and brewing method thereof |
Non-Patent Citations (6)
| Title |
|---|
| AIKATERINI TERMENTZI，ETAL.: "Antioxidant activities of various extracts and fractions of Sorbus domestica fruits at different maturity stages", 《FOOD CHEMISTRY》 * |
| ANNE T. HUKKANEN，ETAL.: "Antioxidant Capacity and Phenolic Content of Sweet Rowanberries", 《J. AGRIC. FOOD CHEM》 * |
| 帕提古丽·马合木提等: "西伯利亚花楸枝条提取物抑菌作用的测定", 《食品科学》 * |
| 帕提古丽•马合木提等: "天山花楸果实的有效成分的初步研究", 《食品科学》 * |
| 樊梓鸾等: "5种野生浆果的抗氧化和抗细胞增殖活性", 《食品科学》 * |
| 白洁等: "天山花楸全株化学成分定性研究", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104622998A (en) * | 2015-01-05 | 2015-05-20 | 辽宁大学 | Applications of fructus sorbus sibirica extract in preparation of lipid-lowering medicament |
| CN106177015A (en) * | 2016-08-01 | 2016-12-07 | 吉林大学 | Sorbus domestica application in preparing xanthine oxidase inhibitor |
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Application publication date: 20130724 |