Background technology
Sucking pest is larger population in the Important Economic insects such as cotton, as cotten aphid, cotton thrips, leafhopper, cotton leaf bug, cotton spider mites, plant hopper, aleyrodid,
Its upper jaw and the lower jaw lancet alternately thrusts in the tissue of plant, sucks juice, causes pathologic or the injury of raw existing property, causes cotton seedling plant short and small, and blade diminishes, and chlorosis reddens, and blade spottiness, incises, and even whole strain is withered or dead; Most kinds are virus disease and phytoplasma disease vector insect, bring secondary disease to crop, all therefore cause every year the heavy loss of Cotton in China.
Cyhalothrin, English name cyhalothrin, molecular formula C
23h
19cIF
3nO
3, chemical name is 2,2-dimethyl-3-(2-chloro-3,3, the fluoro-1-acrylic of 3-tri-) cyclopropane-carboxylic acid-a-cyano group-3-phenoxy group benzyl ester, another name Cyhalothrin, gamma cyhalothrin.Cyhalothrin belongs to pyrethroid bionic Pesticide.Insect and mite class are had to strong tagging and stomach poison function, have permeability and without systemic action, can effectively prevent and treat Lepidoptera, coleoptera, Semiptera and mite pest.Its stable in properties, resistance of rainwater washing against.There is desinsection wide spectrum, the feature that efficient, speed is fast, the lasting period is long.The drug effect lasting period is long, dispenser 1 time, and after 20 days, preventive effect still can reach more than 90%.But this product is moderate toxicity to people and animals, to the high poison of the beneficial organisms such as aquatile, honeybee, silkworm, environment friendly is poor.
First nitro contracting aminoguanidine insecticides of penta Bi Chongguashi China Agricultural University initiative, agriculture chemical registration is checked and approved " penta pyrrole worm guanidine " by name, chemical name is the chloro-3`-picolyl of 1-nitro-3-(6`-)-4-valeraldehyde contracting aminoguanidine, mouse acute oral LD female, great and mighty or powerful
50> 5000mg/kg.BW, belong to micro-poison, be non-stimulated level to lagophthalmos, skin irritatin intensity, skin metamorphosis (sensitization) intensity is I level, belong to weak sensitizer, Salmonella reversion test result is negative, rat acute toxicity is only had to approximately 1/10 (Imidacloprid 450mg/kg.BW) of Imidacloprid.Imidacloprid belongs to high poison level, and penta pyrrole worm guanidine is low toxicity level, lower 10 times than Imidacloprid to the absorption toxicity of honeybee in addition.Result shows, penta pyrrole worm guanidine has efficiently, low toxicity and the good feature of safety, have the architectural feature of anabasine insecticide and sodium-ion channel inhibitor insecticides concurrently, there is desinsection pedigree widely, there is the feature of multiaction target, this product has good control efficiency to multiple suckings pest such as aphid, plant hopper, cotton bollworms, but this product quick-acting is poor, and drug action is slower.
The structural formula of penta pyrrole worm guanidine:
Summary of the invention
A kind of aqueous emulsion of the composition pesticide of preventing and treating cotton aphid and thrips, the described aqueous emulsion by weight percentage of constituent content is counted: penta pyrrole worm guanidine 5~20%, cyhalothrin 5~20%, solvent 10~15%, emulsifier 4~10%, defoamer 0.2~0.5%, antifreezing agent 5~10%, disintegrant 2~6%, surplus is water.
Described solvent is the one in methyl alcohol, ethanol, toluene, dimethylbenzene, acetone.
Prevent and treat an aqueous emulsion for the composition pesticide of cotton aphid and thrips, described emulsifier is vegetable oil, Chinese honey locust, sodium butylnaphthalenesulfonate, sodium methylene bis-naphthalene sulfonate, sodium alkyl benzene sulfonate, polycarboxylate, phosphate, sodium sulphate, pulls open the one in powder, aliphatic acid polyethenoxy base ether, alkylphenol-polyethenoxy base ether, polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol formaldehyde polymer polyoxyethylene ether.
Prevent and treat an aqueous emulsion for the composition pesticide of cotton aphid and thrips, described defoamer is the one in poly-alkyl silicon oxirane, tributyl phosphate.
Prevent and treat an aqueous emulsion for the composition pesticide of cotton aphid and thrips, described antifreezing agent is the one in ethylene glycol, propane diols, glycerine, glycerine-ether diglycol, isopropyl methyl diglycol.
Prevent and treat an aqueous emulsion for the composition pesticide of cotton aphid and thrips, described disintegrant is the one in bentonite, urea, ammonium sulfate, aluminium chloride, glucose.
A kind of aqueous emulsion of the composition pesticide of preventing and treating cotton aphid and thrips, the described aqueous emulsion by weight percentage of constituent content is counted: penta pyrrole worm guanidine 5%, cyhalothrin 15%, dimethylbenzene 15%, alkylphenol-polyethenoxy base ether 6%, tributyl phosphate 0.2%, ethylene glycol 8%, ammonium sulfate 2%, surplus is water.
Prevent and treat an aqueous emulsion for the composition pesticide of cotton aphid and thrips, for the preparation of the medicine of control agricultural pests cotton aphid and thrips.
Objects of the present invention:
After penta pyrrole worm guanidine and cyhalothrin are composite, can effectively prevent and treat resistance cotten aphid, control efficiency is better than contrast, and lasting period length, preventive effect reach more than 90%, and within the scope of experimental drug, target crop do not had to poisoning.Can effectively solve cyhalothrin environment friendly poor, penta pyrrole worm guanidine quick-acting is poor, the shortcoming that drug action is slower.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated, represents for describing each constituent content weight portion in embodiment.
Embodiment 1:15% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 10%, cyhalothrin 5%, ethanol 15%, sodium butylnaphthalenesulfonate 4%, poly-alkyl silicon oxirane 0.5%, glycerine 5%, ammonium sulfate 5%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 15% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 2:20% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 5%, cyhalothrin 15%, dimethylbenzene 15%, alkylphenol-polyethenoxy base ether 6%, tributyl phosphate 0.2%, ethylene glycol 8%, ammonium sulfate 2%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 20% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 3:25% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 5%, cyhalothrin 20%, methyl alcohol 10%, sodium methylene bis-naphthalene sulfonate 10%, tributyl phosphate 0.4%, propane diols 8%, aluminium chloride 2%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 25% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 4:30% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 5%, cyhalothrin 25%, acetone 15%, pull open powder 8%, tributyl phosphate 0.3%, ethylene glycol 8%, ammonium sulfate 4%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 30% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 5:40% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 20%, cyhalothrin 20%, dimethylbenzene 15%, alkylphenol polyoxyethylene 10%, poly-alkyl silicon oxirane 0.2%, glycerine 6%, urea 2%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 40% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 6:10% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 5%, cyhalothrin 5%, acetone 12%, alkyl phenol formaldehyde polymer polyoxyethylene ether 4%, poly-alkyl silicon oxirane 0.2%, glycerine-ether diglycol 10%, glucose 6%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 10% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 7:24% penta pyrrole worm guanidine cyhalothrin water emulsion
Penta pyrrole worm guanidine 19%, cyhalothrin 5%, methyl alcohol 13%, alkylphenol-polyethenoxy base ether 6%, poly-alkyl silicon oxirane 0.3%, isopropyl methyl diglycol 7%, bentonite 4%, surplus is water.Above raw material is prepared routinely to the method for aqueous emulsion and mixed 10% penta pyrrole worm guanidine cyhalothrin water emulsions of system.
Embodiment 1-5 and 20% penta pyrrole worm guanidine aqueous emulsions, 10% cyhalothrin water emulsion are done to contrast experiment.Taking resistance cotten aphid (claiming again cotton aphid) as experimental subjects, 7 days intervals of each dispenser, dispenser 3 times altogether, with after last dispenser 3 days, 7 days, investigates respectively the cotten aphid state of an illness for 21 days and calculates control efficiency.Control efficiency is calculated as follows:
Control efficiency (%)=(1-T
ac
b/ T
bc
a) × 100%
T in formula
afor the individual amount of survival before treatment region control; T
bfor the individual amount of survival after treatment region control; C
afor the individual amount of survival after the control of check plot; C
bfor the individual amount of survival before the control of check plot.
Field efficacy experimental result is in table 1.
Table 1 is prevented and treated the experimental result of resistance cotten aphid
As can be seen from Table 1, after penta pyrrole worm guanidine and cyhalothrin are composite, can effectively prevent and treat resistance cotten aphid, control efficiency is better than contrast, and lasting period length, preventive effect reach more than 90%, and within the scope of experimental drug, target crop do not had to poisoning.
Embodiment 1-5 and 20% penta pyrrole worm guanidine SC, 10% cyhalothrin water emulsion are done to contrast experiment.Taking thrips on cotton as experimental subjects, 7 days intervals of each dispenser, dispenser 3 times altogether, with after last dispenser 3 days, 7 days, investigates respectively the thrips state of an illness for 21 days and calculates control efficiency.Control efficiency is calculated as follows:
Control efficiency (%)=(1-T
ac
b/ T
bc
a) × 100%
T in formula
afor the individual amount of survival after treatment region control; T
bfor the individual amount of survival after treatment region control; C
afor the individual amount of survival after the control of check plot; C
bfor the individual amount of survival before the control of check plot.
Field efficacy experimental result is in table 2.
Table 2 is prevented and treated the experimental result of thrips on resistant cotton
As can be seen from Table 2, after penta pyrrole worm guanidine and cyhalothrin are composite, can effectively prevent and treat thrips on cotton, control efficiency is better than contrast, and lasting period length, preventive effect reach 90%, and within the scope of experimental drug, target crop do not had to poisoning.
The result of consolidated statement 1 and table 2 can find out, the present invention is in the situation that effective content is less than or equal to single dose, and its insecticidal activity medicine is higher than each active component single dose, illustrates that Pesticidal combination of the present invention and preparation thereof can effectively prevent and treat aphid and thrips on cotton.
Embodiment 6 penta pyrrole worm guanidine toxicity mixed with cyhalothrin surveyed
One, reagent agent: the 95% penta former medicines of pyrrole worm guanidine, the former medicine of 95% cyhalothrin, methyl-sulfoxide, Tween 80, agar powder.
Two, for examination insect: cotten aphid (Aphis gossypii Glover), pick up from academy of agricultural sciences of Henan Province, Yuanyang County, Henan Province the second Experimental Base, take back more than greenhouse bred for 2 generations with cotton seedling.
Three, medicament preparation: 9 medicament groups are set, and every group of 5 concentration are respectively
In penta pyrrole worm guanidine single dose group, each drug concentration is made as 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, 8 μ g/mL, 16 μ g/mL;
In cyhalothrin single dose group, each drug concentration is made as 5 μ g/mL, 10 μ g/mL, 20 μ g/mL, 40 μ g/mL, 80 μ g/mL;
Above methyl-sulfoxide (DMSO) dissolving for medicament, then adds 80mL0.1% Tween solution and is diluted to above concentration.
7 groups of penta pyrrole worm guanidine and cyhalothrin confections, the active component total concentration of each dose is made as in every group: the weight ratio of 2.5 μ g/mL, 5 μ g/mL, 10 μ g/mL, 20 μ g/mL, 40 μ g/mL each group penta pyrrole worm guanidine and cyhalothrin is followed successively by 2:1,1:1,1:2,1:3,1:4,1:5,1:6.
Four, test method:
Utilize potted plant cultivation cotton seedling access aphid, get the true leaf with 20 aphids, immerse liquid 3s, after taking-up, suck open fire with blotting paper, be placed in the culture dish with wet sponge, 4 repetitions of each processing, be put in insectary and cultivate, condition of culture is L/D=14: 10, T=(26 ± 2) DEG C, RH=(75 ± 5) %, 24h investigates remaining worm amount, calculates lethality, taking concentration logarithm value as dependent variable, lethality logarithm value is that independent variable carries out regression analysis, calculates virulence equation and LC
50value, and then utilize Sun Pei Yun method to calculate and spend coefficient (CTC) together.
Five, experimental result
Table 3 penta pyrrole worm guanidine is mixed with cyhalothrin to cotten aphid toxicity measurement result
As can be seen from Table 3, penta pyrrole worm guanidine has higher virulence to cotten aphid, its LC
50, LC
95and correlation coefficient is respectively 3.89 μ g/mL, 37.35 μ g/mL and 0.9959.LC50, LC95 and the correlation coefficient of cyhalothrin to cotten aphid is respectively 26.17 μ g/mL, 260.47 μ g/mL and 0.9853.Penta pyrrole worm guanidine proportionings different from cyhalothrin (2:1~1:6) are respectively 5.03 μ g/mL, 5.54 μ g/mL, 6.07 μ g/mL, 6.35 μ g/mL, 8.01 μ g/mL, 10.09 μ g/mL and 12.27 μ g/mL to the LC50 value of cotten aphid, LC95 value is respectively 39.66 μ g/mL, 51.47 μ g/mL, 49.47 μ g/mL, 47.56 μ g/mL, 60.02 μ g/mL, 97.32 μ g/mL and 140.19 μ g/mL, and correlation coefficient is respectively 0.9736,0.9917,0.9950,0.9828,0.9838,0.9729 and 0.9702.
Use the abundant method of Sun Yun to calculate the co-toxicity coefficient of different medicament proportionings, can be found out by result of the test, penta pyrrole worm guanidine and cyhalothrin have and are added or synergistic effect cotten aphid to test set ratio proportioning, different proportionings (2:1~1:6) are respectively 107.92,122.31,148.25,169.52,152.32,132.73 and 117.33 to the co-toxicity coefficient of cotten aphid, wherein, with the co-toxicity coefficient maximum of penta pyrrole worm guanidine: cyhalothrin=1:3, synergy is the most remarkable.