CN103196936B - A kind of based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment - Google Patents
A kind of based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment Download PDFInfo
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Abstract
A kind of based on<i>γ</i>-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, belongs to technical field of quick detection of pathogenic bacteria for food safety. The present invention depends on the magnetic resonance detection method that may be used for pathogenic bacterium in food liquid sample of foundation, utilizes 1 anti-specific binding object bacteria, utilizes<i>γ</i>-Fe2O3Au composite nano materials prepares the object bacteria of the 2 anti-marks of anti-specific immunity magnetic bead enrichment pointedly 1, utilizes<i>γ</i>-Fe2O3Whether the paramagnetic of Au composite nanoparticle and superparamagnetic characteristic, on the impact in nucleus magnetic resonance deamplification relaxation time, detect out in sample containing object bacteria.<i>γ</i>-Fe2O3Au composite nanoparticle is enrichment means is also detection probes. Different concrete corresponding relations is: paramagnetic immunomagnetic beads, demonstrate linear relationship under certain condition, namely immunomagnetic beads content is big, and spin-lattice relaxation time or the spin spin relaxation time value of sample are more little, can detection by quantitative object bacteria in certain limit. The method may be used for the quick detection of harmful pathogenic bacteria in food samples, such that it is able to as the rapid screening of large quantities of sample to be checked.
Description
Technical field
The present invention relates to the method for quick of a kind of pathogenic bacterium, particularly relate to a kind of based on γ-Fe2O3The method for quick of the food-borne pathogens of Au composite nanoparticle.
Background technology
Ultimate principle: monoclonal antibody or antigen molecule are combined by covalent linkage with enzyme molecule, this kind of combination can not change immunological characteristic and the biological activity of monoclonal antibody, antigen and enzyme, and the monoclonal antibody of specificity only can be combined with the antigen of specificity. Its main principle steps: 1. the 1st antibody adding object bacteria in sample to be checked, if there is object bacteria, then forms 1 anti-mixture with 1 anti-binding; 2. utilize seed mediated growth method to prepare magnetic γ-Fe2O3Au core-shell nano, realized by modified antibodies surface-functionalized, be coupled at magnetic bead surfaces by anti-for 1 anti-antibody that is 2, form specific immunity magnetic bead, and unnecessary avtive spot is closed. 2. adopt certain method to be fixed on enzyme plate surface the anti-monoclonal antibody of specificity 1 of another part object bacteria, and unnecessary avtive spot is closed. 3. the 2 anti-specific immunity magnetic beads the 2nd step prepared join in the sample to be checked of the 1st step, for capturing enrichment object bacteria, by externally-applied magnetic field by Beads enrichment out, now, the 1 anti-mixture combining object bacteria and there is no combining target bacterium excessive 1 anti-all can with 2 anti-bindings of magnetic bead surfaces, still mix.4. by the above-mentioned magnetic bead mixed, be added to the 2nd step enzyme plate surface, then captured object bacteria magnetic bead will with enzyme plate surface 1 anti-generation specific binding, formed double antibodies sandwich, with aseptic washed with de-ionized water can will not occur combine magnetic bead wash-out. 5. adopting eluent to wash by the specific nano immunomagnetic beads of the combination on immobilization carrier again, Magneto separate washes ion, solvent. This part magnetic bead is if it is present, be exactly the magnetic bead having captured object bacteria. Due to γ-Fe2O3Au composite nanoparticle has paramagnetic and superparamagnetic characteristic, very responsive for resonance instrument, for other molecules, and the γ-Fe of trace2O3Au composite nanoparticle can the spin lattice relaxation time of the aseptic deionized water of much slower and spin spin relaxation time, and aseptic deionized water is under certain even field intensity, spin-lattice relaxation time or spin spin relaxation time are fixing. Elutriant is placed in nuclear magnetic resonance analyser, compares with aseptic deionized water control group. The explanation significantly occurring spin-lattice relaxation time or spin spin relaxation time value to reduce has magnetic bead to exist, thus has pathogenic bacterium detection in side light food samples. Magnetic bead content and spin-lattice relaxation time or spin spin relaxation time decreasing value are direct ratio. By adding mark, it is possible to detection by quantitative object bacteria. γ-Fe in the method2O3Au composite nanoparticle magnetic bead is the means of separation and concentration, simultaneously γ-Fe2O3The paramagnetic that Au composite nanoparticle has and superparamagnetic characteristic, can be used as again the probe of detection by quantitative. The major advantage of the method is exactly quick, highly sensitive. Relative to microorganism culturing 2-3 days even time of several days of pathogenic bacterium, this method depends primarily on the pretreatment time of sample, and magnetic resonance detection only needs several minutes. Therefore, can doing the positive-selecting of extensive sample to be checked by the method, the positive sample of detection also needs to confirm by microorganism culturing. At present, also do not have document to report this kind of method both at home and abroad, but adopt immunomagnetic beads to carry out the enrichment of pathogenic bacterium, the report of the enrichment etc. of target compound or a lot, but all do not adopt nucleus magnetic resonance to do further detection.
Summary of the invention
It is an object of the invention to provide a kind of based on γ-Fe2O3The method for quick of the NMR food-borne pathogens of Au composite nanoparticle indirect enrichment, for various different food samples is evaluated, the method is the method for harmful pathogenic bacteria in a kind of objective effective detection food, thus greatly reduces the screening time of food samples harmful pathogenic bacteria to a certain extent.
A kind of based on γ-Fe2O3The nuclear magnetic resonance technique of Au composite nanoparticle indirect enrichment detects out the method for the harmful pathogenic bacteria in food fast, utilizes nuclear magnetic resonance analyser to the response sensibility of paramagnetic, superparamagnetic material, it is proposed to NMR relaxation Parameters variation and γ-Fe2O3The correlation metric of Au composite nanoparticle superparamagnetic immunomagnetic beads content. Different pathogenic bacterium detection lower limits is different.
What the method depended on foundation can be used for the immunomagnetic beads enrichment of harmful pathogenic bacteria characteristic, separation in food samples, from the angle of NMR relaxation signal Parameters variation, detects out the harmful pathogenic bacteria in sample; 1 anti-mark of object bacteria is adopted to form 1 anti-mixture; The superparamagnetic immunomagnetic beads adopting coupling 1 anti-antibody that is 2 anti-, it is possible to the specific pathogenetic bacterium in sample is carried out enrichment and is separated;Owing to nuclear magnetic resonance analyser SPIN-LATTICE RELAXATION efficiency and spin-spin relaxivity are to γ-Fe2O3Au composite nanoparticle is very responsive, namely in deionized water, there is the superparamagnetic γ-Fe of trace2O3Au composite nanoparticle, then the spin-lattice relaxation time of water or spin spin relaxation time will significantly decline. Under certain condition, the paramagnetic properties of superparamagnetic immunomagnetic beads makes the spin-lattice relaxation time of nucleus magnetic resonance or spin spin relaxation time produce linear reduction. Gone out the immunomagnetic beads content in sample by detection by quantitative, thus indirectly quantify the detrimental bacterial content in food samples. The pathogenic bacterium content detected out is linearly relevant to magnetic bead content, and degree of fitting is better. Final taking the corresponding relation between superparamagnetic immunomagnetic beads and pathogenic bacterium as tie, it is determined that the pathogenic bacterium colony number in food samples.
The present invention realizes like this, and step is as follows:
1) in sample to be checked, adding the 1st antibody of object bacteria, if there is object bacteria, then forming 1 anti-mixture with 1 anti-binding;
2) preparation of the 2 anti-i.e. antibody immune magnetic beads of the 1st antibody;
3) it is fixed on enzyme plate surface by anti-for object bacteria 1;
4) immunomagnetic beads enrichment object bacteria, and be separated: by the 2nd) the obtained 2 anti-specific immunity magnetic beads of step add the sample to be checked of the 1st step, fully mix concussion, by applying externally-applied magnetic field after capturing object bacteria, then magnetic bead is just pooled to magnetic field on one side, if siphoning away supernatant liquor then to isolate in magnetic bead sample to be checked and have object bacteria, then by the enrichment of magnetic bead institute, add the magnetic bead suspension liquid that deionized water aseptic on a small quantity then forms object bacteria; Now, the 1 anti-mixture combining object bacteria and there is no combining target bacterium excessive 1 anti-all can with 2 anti-bindings of magnetic bead surfaces, still mix.
4) the magnetic bead suspension liquid of enrichment being added on the enzyme plate securing 1 anti-monoclonal antibody of the 3rd step making, if there is object bacteria, forming double antibodies sandwich; By aseptic washed with de-ionized water, then the magnetic bead not grabbing object bacteria is just washed off, if there is not object bacteria, then all magnetic beads are all washed off;
5), after, wash with the magnetic bead of eluent by the double antibodies sandwich on enzyme plate, drop off son and solvent by aseptic washed with de-ionized water by the method for externally-applied magnetic field separation magnetic bead, if also there is magnetic bead is exactly the magnetic bead catching object bacteria;
6) the 5th) magnetic bead of step gained, add the suspension liquid that aseptic deionized water forms magnetic bead, the relaxation time carrying out nucleus magnetic resonance measures, taking aseptic deionized water as blank, the relaxation time of the suspension liquid recorded compares aseptic deionized water remarkable reduction, then illustrate containing magnetic bead, thus have object bacteria in indirect proof sample, the decline of the amount of magnetic bead and spin-lattice relaxation time or spin spin relaxation time is direct ratio, is indirectly quantified the amount of object bacteria by quantitative magnetic bead.
Described nano material is γ-Fe2O3Namely Au composite nanoparticle is the magnetic core-shell composite nanoparticle material of shell material taking gold, and nanometer particle size is less than 1000 nanometers.
The final detection evaluation method of described object bacteria is the change in the relaxation time based on nuclear magnetic resonance technique.
In the described relaxation time, refer to spin-lattice relaxation time and spin spin relaxation time.
In the described relaxation time, spin spin relaxation time adopts 180-τ-90 pulse methods to measure, and spin-lattice relaxation time adopts CPMG sequence method to measure.
The monoclonal antibody of the anti-finger object bacteria in described 1, the antibody of 2 anti-finger the 1st antibody.
The useful effect of the present invention: the present invention provides a kind of method objectively detecting out the harmful pathogenic bacteria in food fast, it is characterized in that depending on the magnetic resonance detection method that can be used for detection of superparamagnetic immunomagnetic beads of foundation. Harmful pathogenic bacteria in food can objective be detected by the method effectively, confirms compared to the biological culture of pathogenic bacterium, and the method has the advantage of detection fast, it is possible to for the rapid screening of extensive sample.
Embodiment
Example 1
Inspection food samples measures it whether containing harmful pathogenic bacteria listeria bacteria.
1.2 anti-specific immunity magnetic beads preparations: the rabbit anti-igg monoclonal antibody of 1 anti-employing listeria bacteria, 2 resist the goat anti-rabbit igg for listeria bacteria, it is possible to be monoclonal antibody can also be resist more. γ-Fe2O3Nanoparticle can adopt chemical coprecipitation to prepare, it is possible to adopts additive method preparation. As by the γ-Fe after 1mL dilution2O3Mix with same volume 0.1mol/L Trisodium Citrate, it is diluted to 20mL, under stirring, add excessive 80mmol/LNH2(weight ratio) HAuCl that adds 0.1% is dripped again after OHHCl solution4Solution 2mL, namely obtains γ-Fe through washing separation after reaction 1h2O3Au。
Immunity γ-Fe2O3Prepared by Au: γ-Fe2O3After adding excessive goat anti-rabbit igg antibody after Au is concentrated, hatch, washing, add excessive BSA and close surfactivity room, washing, resuspended floating, it is kept at 4 DEG C stand-by. Also following method can be adopted: adopt the 200 micro-2mmol of liter disulfide group-succinimide-propionic esters (DSP) nanometer gold to be modified (DMSO, dimethyl sulfoxide (DMSO) dilution DSP). Add listeria bacteria goat anti-rabbit igg antibody, by 100 μ L100 μ g/mL goat anti-rabbit igg antibodies by e be fixed on Au and 37 C hatch 45min. Add 1% bovine serum albumin (BSA), 22 C, 1 hour, remaining avtive spot is carried out close and dry.
2.1 anti-monoclonal antibodies are fixed: can adopt conventional enzyme plate fixing means, it is also possible to adopt following method. With clean cover glass 5 × 5mm2Square, coating equipment first sprays one layer of Cr (2 4nm) in order to help fixing gold. It is used in surface sputtering spray last layer nanometer gold again, then adopts the 200 micro-2mmol of liter disulfide group-succinimide-propionic esters (DSP) nanometer gold to be modified (DMSO, dimethyl sulfoxide (DMSO) dilution DSP). Add the rabbit anti-igg mono-clonal monoclonal antibody of listeria bacteria, fix on a glass also 37 C by 100 μ L100 μ g/mL monoclonal antibodies by e and hatch 45min. Add 1% bovine serum albumin (BSA), 22 C, 1 hour, avtive spot remaining on plate is carried out close and dry.
3. food samples is carried out pre-treatment, adopt FDA to increase bacterium method if desired, sample is filtered, increases the pre-treatment such as bacterium activation, obtain sample to be checked. Sample to be checked will add the 1st antibody, the rabbit anti-igg of listeria bacteria. If there is listeria bacteria in sample to be checked, will with the 1 anti-anti-mixture of formation 1. Fully shake after the goat anti-rabbit igg magnetic bead of the 2nd obtained for the 1st step antibody listeria bacteria is added. Upper magnetic force frame separation magnetic bead, adds the suspension liquid that deionized water aseptic on a small quantity obtains magnetic bead. Now, if sample to be checked has target listeria bacteria, then by the interaction of the 2nd antibody and the 1st antibody, thus catch this mixture, reach the object of object bacteria enrichment. Now, the 1 anti-mixture combining object bacteria and there is no combining target bacterium excessive 1 anti-all can with 2 anti-bindings of magnetic bead surfaces, still mix.The magnetic bead suspension liquid of this enrichment is added on 1 anti-enzyme plate prepared by the 2nd step, then magnetic bead emulsion combines listeria bacteria magnetic bead can further with the monoclonal antibody generation specific binding on enzyme plate, formed double antibodies sandwich structure. Now by aseptic washed with de-ionized water, so that it may so that not magnetic bead in conjunction with listeria bacteria is washed. The magnetic bead just only combining listeria bacteria remaining on enzyme plate.
4. with elutriant (methyl alcohol etc.), the magnetic bead combining listeria bacteria on enzyme plate is eluted. Upper magnetic force frame, separation magnetic bead also cleans 1-2 time, ion, solvent is washed. The solution obtained, measures spin-lattice relaxation time or the spin spin relaxation time of solution by nuclear magnetic resonance analyser (NMR20, Niu Mai company). Taking aseptic deionized water as blank, the spin-lattice relaxation time that solution records or spin spin relaxation time are compared with blank, there were significant differences, illustrate and solution has magnetic bead exist, thus illustrate and sample has listeria bacteria, the decline value of the amount of magnetic bead and spin-lattice relaxation time or spin spin relaxation time is direct ratio. The bright magnetic bead of more speaking more of decline is more many, thus side light listeria bacteria is more many, can the number of object bacteria in detection by quantitative sample by adding mark checking.
Embodiment
Example 2
Whether measure food samples containing harmful pathogenic bacteria Escherichia coli O 157: H7.
1.2 anti-specific immunity magnetic beads preparations: the rabbit anti-igg monoclonal antibody of 1 anti-employing O157:H7,2 resist the goat anti-rabbit igg for O157:H7, it is possible to be monoclonal antibody can also be resist more. γ-Fe2O3Nanoparticle can adopt chemical coprecipitation to prepare, it is possible to adopts additive method preparation. As by the γ-Fe after 1mL dilution2O3Mix with same volume 0.1mol/L Trisodium Citrate, it is diluted to 20mL, under stirring, add excessive 80mmol/LNH2(weight ratio) HAuCl that adds 0.1% is dripped again after OHHCl solution4Solution 2mL, namely obtains γ-Fe through washing separation after reaction 1h2O3Au。
Immunity γ-Fe2O3Prepared by Au: γ-Fe2O3After adding excessive O157:H7 goat anti-rabbit igg antibody after Au is concentrated, hatch, washing, add excessive BSA and close surfactivity room, washing, resuspended floating, it is kept at 4 DEG C stand-by. Also following method can be adopted: adopt the 200 micro-2mmol of liter disulfide group-succinimide-propionic esters (DSP) nanometer gold to be modified (DMSO, dimethyl sulfoxide (DMSO) dilution DSP). Add O157:H7 goat anti-rabbit igg antibody, by 100 μ L100 μ g/mL goat anti-rabbit igg antibodies by e be fixed on Au and 37 C hatch 45min. Add 1% bovine serum albumin (BSA), 22 C, 1 hour, avtive spot remaining on plate is carried out close and dry.
2.1 anti-monoclonal antibodies are fixed: can adopt conventional enzyme plate fixing means, it is also possible to adopt following method. With clean cover glass 5 × 5mm2Square, coating equipment first sprays one layer of Cr (2 4nm) in order to help fixing gold. It is used in surface sputtering spray last layer nanometer gold again, then adopts the 200 micro-2mmol of liter disulfide group-succinimide-propionic esters (DSP) nanometer gold to be modified (DMSO, dimethyl sulfoxide (DMSO) dilution DSP). Add O157:H7 rabbit anti-igg monoclonal antibody, fix on a glass also 37 C by 100 μ L100 μ g/mL monoclonal antibodies by e and hatch 45min. Add bovine serum albumin avtive spot remaining on plate is carried out close and dry.
3. food samples carried out pre-treatment, sample is filtered, increase the pre-treatment such as bacterium activation, obtain sample to be checked.Sample to be checked will add the 1st antibody, the rabbit anti-igg of O157:H7. If there is O157:H7 in sample to be checked, will with the 1 anti-anti-mixture of formation 1. Fully shake after the goat anti-rabbit igg antibody magnetic bead of the 2nd obtained for the 1st step antibody O157:H7 is added. Upper magnetic force frame separation magnetic bead, adds the emulsion that a small amount of water obtains magnetic bead. Now, if sample to be checked has target Escherichia coli O 157: H7, then by the interaction of the 2nd antibody and the 1st antibody, thus catch this mixture, reach the object of object bacteria enrichment. Now, the 1 anti-mixture combining object bacteria and there is no combining target bacterium excessive 1 anti-all can with 2 anti-bindings of magnetic bead surfaces, still mix. The magnetic bead suspension liquid of this enrichment is added on 1 anti-enzyme plate prepared by the 2nd step, then magnetic bead suspension liquid combines Escherichia coli O 157: the magnetic bead of H7 can further with the monoclonal antibody generation specific binding on enzyme plate, formed double antibodies sandwich structure. Now by aseptic washed with de-ionized water, so that it may with by conjunction with Escherichia coli O 157: the magnetic bead of H7 is washed. The magnetic bead just only combining Escherichia coli O 157: H7 remaining on enzyme plate.
4. enzyme plate will combine Escherichia coli O 157 with elutriant (methyl alcohol etc.): the magnetic bead of H7 elutes. Upper magnetic force frame, separation magnetic bead and by aseptic washed with de-ionized water 1-2 time, ion, solvent are washed. The solution obtained, with nuclear magnetic resonance analyser (NMR20, Niu Mai company) measure spin-lattice relaxation time or the spin spin relaxation time of solution, take deionized water as blank, the spin-lattice relaxation time that solution records or spin spin relaxation time compare with blank, and there were significant differences, illustrate and have magnetic bead to exist in solution, thus illustrate and sample has Escherichia coli O 157: H7, the decline value of the amount of magnetic bead and spin-lattice relaxation time or spin spin relaxation time is direct ratio. The bright magnetic bead of more speaking more of decline is more many, thus side light Escherichia coli O 157: H7 is more many, can the number of object bacteria in detection by quantitative sample by adding mark checking.
Claims (5)
1. one kind based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, its characterization step is as follows:
1) 1 anti-binding of object bacteria and object bacteria is formed 1 anti-mixture;
2) the anti-antibody of object bacteria 1, i.e. the preparation of 2 anti-specific immunity magnetic beads;
3) another part object bacteria 1 is resisted fixing on enzyme plate;
4) immunomagnetic beads enrichment object bacteria, and be separated: by the 2nd) the obtained 2 anti-specific immunity magnetic beads of step join the 1st) step combines 1 anti-sample to be checked, fully mix concussion, by applying externally-applied magnetic field after capturing object bacteria, then magnetic bead is just pooled to magnetic field on one side, siphons away supernatant liquor and then can isolate magnetic bead; If having object bacteria in sample to be checked, first object bacteria forms mixture with 1 anti-binding, after adding 2 diamagnetic pearls, by 2 anti-and 1 anti-interactions, thus catch 1 anti-mixture, by the enrichment of magnetic bead institute, add the magnetic bead suspension liquid that deionized water aseptic on a small quantity then forms object bacteria;
5) the magnetic bead suspension liquid of enrichment being added to the 3rd) step secures on 1 anti-enzyme plate, if there is object bacteria, forming double antibodies sandwich, by aseptic washed with de-ionized water, then the magnetic bead not grabbing object bacteria is just washed off, if there is not object bacteria, then all magnetic beads are all washed off;
6), after, wash with the magnetic bead of eluent by the double antibodies sandwich on enzyme plate, by the method for externally-applied magnetic field separation magnetic bead, drop off son and solvent by aseptic washed with de-ionized water, if also there is magnetic bead is exactly the magnetic bead catching object bacteria;
7) the 6th) magnetic bead of step gained, adds the suspension liquid that aseptic deionized water forms magnetic bead, and the relaxation time carrying out nucleus magnetic resonance measures;Under fixing field intensity, the spin-lattice relaxation time of aseptic deionized water or spin spin relaxation time are steady state values, taking aseptic deionized water as blank, the relaxation time of the suspension liquid recorded compares aseptic deionized water remarkable reduction, then illustrate containing magnetic bead, thus indirect proof sample has object bacteria, the decline value of the amount of magnetic bead and spin-lattice relaxation time or spin spin relaxation time is direct ratio, indirectly quantifies the amount of object bacteria by adding demarcation amount magnetic bead.
2. according to claim 1 based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, is characterized in that described magnetic bead is γ-Fe2O3Namely Au composite nanoparticle is the magnetic core-shell composite nanoparticle material of shell material taking gold, and nanometer particle size is less than 1000 nanometers.
3. according to claim 1 based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, is characterized in that described spin-lattice relaxation time adopts 180-τ-90 pulse methods to measure, and described spin spin relaxation time adopts CPMG sequence method to measure.
4. according to claim 1 based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, is characterized in that object bacteria first forms mixture with 1 anti-binding, and with 2 anti-specific immunity enrichment with magnetic bead, separation, 1 has resisted signal amplification.
5. according to claim 1 based on γ-Fe2O3The NMR food-borne pathogen rapid detection of Au composite nanoparticle indirect enrichment, 1 anti-finger object bacteria monoclonal antibody, the 2 anti-antibody of anti-finger 1.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726535A (en) * | 2008-10-24 | 2010-06-09 | 北京朔望科技有限公司 | Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial |
| WO2011150212A2 (en) * | 2010-05-26 | 2011-12-01 | The General Hospital Corporation | Magnetic nanoparticles |
| CN102323408A (en) * | 2011-05-31 | 2012-01-18 | 上海师范大学 | Method for rapid detection of enterobacter sakazakii |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726535A (en) * | 2008-10-24 | 2010-06-09 | 北京朔望科技有限公司 | Time-resolved remanence relaxation detector and application thereof in detection of superparamagnetic nanomaterial |
| WO2011150212A2 (en) * | 2010-05-26 | 2011-12-01 | The General Hospital Corporation | Magnetic nanoparticles |
| CN102323408A (en) * | 2011-05-31 | 2012-01-18 | 上海师范大学 | Method for rapid detection of enterobacter sakazakii |
Non-Patent Citations (2)
| Title |
|---|
| One-Step, Nanoparticle-Mediated Bacterial Detection with Magnetic Relaxation;Charalambos Kaittanis,et.al.;《NANO LETTERS》;20061229;第7卷(第2期);380-383 * |
| 超顺磁纳米颗粒弛豫时间传感技术在生化分析中的研究进展;陈翊平 等;《分析测试学报》;20111130;第30卷(第11期);1224-1229 * |
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