CN103190523A - Yangtao seed meal isolated protein and extraction method thereof - Google Patents
Yangtao seed meal isolated protein and extraction method thereof Download PDFInfo
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- CN103190523A CN103190523A CN2013101521618A CN201310152161A CN103190523A CN 103190523 A CN103190523 A CN 103190523A CN 2013101521618 A CN2013101521618 A CN 2013101521618A CN 201310152161 A CN201310152161 A CN 201310152161A CN 103190523 A CN103190523 A CN 103190523A
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- chinese gooseberry
- seed dregs
- protein isolate
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Abstract
The invention discloses a yangtao seed meal isolated protein. The yangtao seed meal isolated protein has a molecular weight of 20000 to 58000 Da, an emulsifying activity index of 92.38+/-1.27 m<2>/g and a foaming characteristic of 22.23+/-4.32% when the pH is 7.5, and an emulsifying activity index of 18.34+-0.69 and a foaming characteristic of 34.93+/-0.72% when the pH is 4.0. Cheap and easily obtained material is utilized in a simple and easily operated processing technology, and protein powder is extracted from yangtao seed meal as raw material; the obtained yangtao seed meal isolated protein can be used as a functional nutritional supplement and also can be added into food and medicine as a functional base material, and has wide application and promotion values; and the utilization value of yangtao seed meal is promoted.
Description
Technical field
The present invention relates to a kind of Chinese gooseberry seed dregs of rice protein isolate and extracting method thereof.
Background technology
Along with improving constantly of people's living standard, the daily amount of protein is increasing, excessive edible animal proteins and disease such as the obesity that causes, high fat of blood more and more is subjected to people's attention.In addition, for area or the vegetarianism that animal protein lacks, the main path that replenishes beyond doubt additional body nutrition of vegetable protein.Vegetable protein is through arranging in pairs or groups mutually, learning from other's strong points to offset one's weaknesses, and various amino acid mix ingests, and makes nutritive value improve greatly, extremely consumers in general's favor.Therefore, be planting property of development of raw materials albumen with the vegetable seeds, not only can realize the efficient utilization of resource, also provide a new way for the novel protein development of food.
Kiwi berry (
Actinidia ChinensisPlanch) be a kind of fallen leaves liana, belong to the Actinidiaceae actinidia in Plant Taxonomy.China is the country of origin of Kiwi berry, and variety source is abundant, and whole world Kiwi berry has kind more than 66, and China just has 62 kinds, mainly is distributed in ground such as China Shaanxi, Hunan, Henan, and wherein Shaanxi Province is the major production areas of Chinese Kiwi berry.According to incompletely statistics, Shaanxi Province's Kiwi berry cultivated area reached 63.81 ten thousand mu in 2010, and 51.08 ten thousand tons of output account for 1/3 of whole world total output, and cultivated area and output all are the first in the nation, and it is about 70% to account for national total output, has special resource advantage.
Kiwifruit fruit delicate fragrance deliciousness, sweet and sour taste, except being rich in vitamin C and potassium, magnesium, cellulose, also contain folic acid, carrotene, calcium, lutern, amino acid, natural inositol etc., have value such as very high nutrition, medical treatment, health care, become one of characteristic fruit that competitively develops in recent years countries in the world.Actinidia is in climacteric type fruit, and very easily deliquescing is rotten after gathering, so except part was used for eating raw, the overwhelming majority was processed to semi-finished product such as dried fruit, can, fruit juice.Along with the continuous expansion of Kiwi berry processing industry, the amount of the accessory substance that produces in the process---Chinese gooseberry seed is also increasing.Single fruit on average contains about 900 of seeds in the kiwifruit fruit, and the per kilogram fruit contains dry seed 49 ~ 150 g approximately.At present, except a spot of Chinese gooseberry seed was used as animal feed and oil expression, the overwhelming majority was dropped as discarded object, and this has not only caused the wasting of resources, also environment has been caused certain pollution.How to utilize living resources efficiently, the comprehensive utilization ratio of raising agricultural byproducts, the recycling and sustainable development of realization living resources (comprising agricultural resource) are the focuses of countries in the world research.Therefore, a large amount of discarded Chinese gooseberry seed dregs of rice of rational exploitation and utilization are problem demanding prompt solutions.
Summary of the invention
One of purpose of the present invention is to provide a kind of Chinese gooseberry seed dregs of rice protein isolate.
Another object of the present invention is to provide the extracting method of above-mentioned Chinese gooseberry seed dregs of rice protein isolate.
Implementation procedure of the present invention is as follows:
A kind of Chinese gooseberry seed dregs of rice protein isolate, its molecular weight is 20000 ~ 58000 Da; Emulsifying activity index 92.38 ± 1.27 m during pH 7.5
2/ g, foaming characteristic 22.23 ± 4.32%; Emulsifying activity index 18.34 ± 0.69 during pH 4.0, foaming characteristic 34.93 ± 0.72%, it extracts by the following method and obtains,
(1) the Chinese gooseberry seed dregs of rice is pulverized, pressed solid-liquid ratio 1:5 ~ 1:12 g/ml and add distilled water, with 0.5 ~ 1.5mol/L NaOH or KOH solution the pH value is transferred to 9.0 ~ 10.5, lixiviate 2 ~ 4 hours, centrifugal collection filtrate;
(2) with 0.5 ~ 1.5mol/L HCl, HNO
3Or H
2SO
4Solution transfers to 3.8 ~ 4.5 with filtrate pH value, leave standstill, centrifugal collecting precipitation, press solid-liquid ratio 1:10 ~ 1:30 g/ml and add distilled water, use 0.5 ~ 1.5 mol/L NaOH or KOH solution to return adjust pH to neutral again, further namely get Chinese gooseberry seed dregs of rice protein isolate after dialysis, the freeze drying, protein extracting ratio is 5.5%-8.5%.
For improving recovery rate, step (1) can be separated the filter residue that obtains and adopt the described method of step (1) to repeat again to extract 2 ~ 3 times.
In the above-mentioned steps (1), centrifugal rotational speed is 4000 ~ 5000 r/min, centrifugation time 15 ~ 20min; In the step (2), centrifugal rotational speed is 8000 ~ 10000 r/min.
Advantage of the present invention and good effect: the present invention adopts processing technology simple to operation with raw material cheap and easy to get, be that raw material extracts albumen powder with the Chinese gooseberry seed dregs of rice, the Chinese gooseberry seed dregs of rice protein isolate that obtains both can be used as the functional nutrient replenishers and had used, can be used as functional base-material again makes an addition in food, the medicine, have and use widely and promotional value, promoted the value of the Chinese gooseberry seed dregs of rice.
Description of drawings
Fig. 1 is the sodium dodecyl sulfate-polyacrylamide gel electrophoresis figure of Chinese gooseberry seed dregs of rice protein isolate, swimming lane M, standard molecular weight marker; Swimming lane 1, soybean protein isolate; Swimming lane 2, Chinese gooseberry seed dregs of rice protein isolate;
Fig. 2 is the dissolubility figure of the different pH of Chinese gooseberry seed dregs of rice protein isolate.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described in further detail.
Get the 100g Chinese gooseberry seed dregs of rice and grind powder, add 500ml distilled water under the room temperature, with the NaOH of 1.0mol/L pH value of solution is transferred to 9.0, magnetic agitation lixiviate 2 hours, the centrifugal 15min of 4800r/min, the removal residue gets supernatant; HCl with 1.0mol/L transfers to 4.5 with supernatant pH then, with the centrifugal 15min of centrifuge 8000r/min, outwells supernatant, collects albumen precipitation.Add 100 ml distilled water in precipitation, with the NaOH regulator solution pH to 7.0 of 1.0mol/L, make fully dissolving of precipitation, the centrifugal 15min of 8000r/min collects supernatant, freeze drying, and protein extracting ratio is 5.54%.
Get the 100g Chinese gooseberry seed dregs of rice and grind powder, add 700ml distilled water under the room temperature, KOH with 1.0mol/L transfers to 10.0 with pH value of solution, magnetic agitation lixiviate 4 hours, centrifugal 20 min of 5000r/min, collect supernatant, then residue is repeated to extract 2 times according to above step, merge the supernatant of 3 extractions; H with 0.5 mol/L
2SO
4Solution transfers to the centrifugal 20min of 3.8,10000r/min with supernatant pH, outwells supernatant, collects albumen precipitation.In precipitation, add 100 ml distilled water, use the pH to 7.0 of the KOH regulator solution of 1.5mol/L, make fully dissolving of precipitation, with the centrifugal 15min of centrifuge 10000r/min, collection supernatant, freeze drying, protein extracting ratio 7.62%.
Get the 100g Chinese gooseberry seed dregs of rice and grind powder, add 1000ml distilled water under the room temperature, with the NaOH of 0.5mol/L pH value of solution is transferred to 9.0, magnetic agitation lixiviate 2 hours, the centrifugal 15min of 4800r/min, the removal residue gets supernatant; Use the HNO of 1.5mol/L then
3PH transfers to 4.5 with supernatant, with the centrifugal 15min of centrifuge 8000r/min, outwells supernatant, collects albumen precipitation.Add 100 ml distilled water in precipitation, with the NaOH regulator solution pH to 7.0 of 0.5mol/L, make fully dissolving of precipitation, the centrifugal 15min of 8000r/min collects supernatant, freeze drying.
Get the 100g Chinese gooseberry seed dregs of rice and grind powder, add 800ml distilled water under the room temperature, with the NaOH of 1.5mol/L pH value of solution is transferred to 10.2, magnetic agitation lixiviate 2 hours, the centrifugal 15min of 4800r/min, the removal residue gets supernatant; Use the H of 0.5mol/L then
2SO
4PH transfers to 3.8 with supernatant, with the centrifugal 15min of centrifuge 8000r/min, outwells supernatant, collects albumen precipitation.Add 100 ml distilled water in precipitation, with the KOH regulator solution pH to 7.0 of 1.5mol/L, make fully dissolving of precipitation, the centrifugal 15min of 8000r/min collects supernatant, freeze drying.
Adopt sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) that the range of molecular weight distributions of embodiment 1 and the 2 Chinese gooseberry seed dregs of rice protein isolates that extract is measured, and compare with soybean protein isolate, found that, the molecular weight of Chinese gooseberry seed dregs of rice protein isolate mainly is distributed in 20000 ~ 25000Da, 35000 ~ 38000Da, three section (Fig. 1 of 48000 ~ 58000Da, swimming lane 1), obviously be different from soybean protein isolate (Fig. 1, swimming lane 2).
Functional characteristic to embodiment 1 and the 2 Chinese gooseberry seed dregs of rice protein isolates that extract comprises: dissolubility, emulsibility, foaming characteristic etc. are measured, and compare with the correlation properties of soybean protein isolate, found that, the dissolving pH that Chinese gooseberry seed is minimum is 3.0, and the minimum dissolving pH of soybean protein isolate is 4.0, when pH 9.0, the dissolubility of Chinese gooseberry seed protein isolate is 4 times (Fig. 2) of soybean protein isolate; Emulsibility comparison shows that under pH 4.0 and 7.5 two kinds of conditions of pH, the emulsifying activity index of Kiwi berry protein isolate and emulsion stability all are higher than soybean protein isolate; But the foaming characteristic difference of two kinds of protein isolates little (table 1), above example explanation Kiwi berry protein isolate has good processing characteristics, is worth further exploitation.
Claims (6)
1. Chinese gooseberry seed dregs of rice protein isolate, its molecular weight is 20000 ~ 58000 Da; Emulsifying activity index 92.38 ± 1.27 m during pH 7.5
2/ g, foaming characteristic 22.23 ± 4.32%; Emulsifying activity index 18.34 ± 0.69 during pH 4.0, foaming characteristic 34.93 ± 0.72%, it extracts by the following method and obtains,
(1) the Chinese gooseberry seed dregs of rice is pulverized, pressed solid-liquid ratio 1:5 ~ 1:12 g/ml and add distilled water, with 0.5 ~ 1.5mol/L NaOH or KOH solution the pH value is transferred to 9.0 ~ 10.5, lixiviate 2 ~ 4 hours, centrifugal collection filtrate;
(2) with 0.5 ~ 1.5mol/L HCl, HNO
3Or H
2SO
4Solution transfers to 3.8 ~ 4.5 with filtrate pH value, leave standstill, centrifugal collecting precipitation, press solid-liquid ratio 1:10 ~ 1:30 g/ml and add distilled water, use 0.5 ~ 1.5 mol/L NaOH or KOH solution to return adjust pH to neutral again, further namely get Chinese gooseberry seed dregs of rice protein isolate after dialysis, the freeze drying.
2. the extracting method of the described Chinese gooseberry seed dregs of rice of claim 1 protein isolate may further comprise the steps:
(1) the Chinese gooseberry seed dregs of rice is pulverized, pressed solid-liquid ratio 1:5 ~ 1:12 g/ml and add distilled water, with 0.5 ~ 1.5mol/L NaOH or KOH solution the pH value is transferred to 9.0 ~ 10.5, lixiviate 2 ~ 4 hours, centrifugal collection filtrate;
(2) with 0.5 ~ 1.5mol/L HCl, HNO
3Or H
2SO
4Solution transfers to 3.8 ~ 4.5 with filtrate pH value, leave standstill, centrifugal collecting precipitation, press solid-liquid ratio 1:10 ~ 1:30 g/ml and add distilled water, use 0.5 ~ 1.5 mol/L NaOH or KOH solution to return adjust pH to neutral again, further namely get Chinese gooseberry seed dregs of rice protein isolate after dialysis, the freeze drying.
3. the extracting method of Chinese gooseberry seed dregs of rice protein isolate according to claim 2, it is characterized in that: Chinese gooseberry seed dregs of rice protein isolate recovery rate is 5.5%-8.5%.
4. the extracting method of Chinese gooseberry seed dregs of rice protein isolate according to claim 2 is characterized in that: step (1) is separated the filter residue that obtains adopt the described method of step (1) to repeat to extract 2 ~ 3 times.
5. the extracting method of Chinese gooseberry seed dregs of rice protein isolate according to claim 2, it is characterized in that: in the step (1), centrifugal rotational speed is 4000 ~ 5000 r/min, centrifugation time 15 ~ 20min.
6. the extracting method of Chinese gooseberry seed dregs of rice protein isolate according to claim 2, it is characterized in that: in the step (2), centrifugal rotational speed is 8000 ~ 10000 r/min.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1635136A (en) * | 2003-12-30 | 2005-07-06 | 北京市食品研究所 | Method for preparing plant separated protein |
| CN1806565A (en) * | 2006-02-14 | 2006-07-26 | 湖南老爹农业科技开发股份有限公司 | Method for preparing kiwi fruit seeds biscuit |
| CN1976595A (en) * | 2004-02-17 | 2007-06-06 | 伯康营养科学(Mb)公司 | Preparation of canola protein isolate and use in aquaculture |
| CN101785507A (en) * | 2010-03-29 | 2010-07-28 | 陕西科技大学 | Method for extracting oil and protein of peach kernel |
-
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- 2013-04-27 CN CN2013101521618A patent/CN103190523A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1635136A (en) * | 2003-12-30 | 2005-07-06 | 北京市食品研究所 | Method for preparing plant separated protein |
| CN1976595A (en) * | 2004-02-17 | 2007-06-06 | 伯康营养科学(Mb)公司 | Preparation of canola protein isolate and use in aquaculture |
| CN1806565A (en) * | 2006-02-14 | 2006-07-26 | 湖南老爹农业科技开发股份有限公司 | Method for preparing kiwi fruit seeds biscuit |
| CN101785507A (en) * | 2010-03-29 | 2010-07-28 | 陕西科技大学 | Method for extracting oil and protein of peach kernel |
Non-Patent Citations (2)
| Title |
|---|
| 吴标等: "脱脂猕猴桃籽粕蛋白质提取工艺条件研究", 《四川食品与发酵》, no. 5, 25 October 2007 (2007-10-25), pages 35 - 38 * |
| 李加兴等: "猕猴桃籽粕蛋白提取工艺研究", 《中国食品学报》, vol. 6, no. 6, 30 December 2006 (2006-12-30) * |
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Application publication date: 20130710 |