CN103190445B - Acid washing sterilization disinfectant and use method thereof - Google Patents
Acid washing sterilization disinfectant and use method thereof Download PDFInfo
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- CN103190445B CN103190445B CN201310133017.XA CN201310133017A CN103190445B CN 103190445 B CN103190445 B CN 103190445B CN 201310133017 A CN201310133017 A CN 201310133017A CN 103190445 B CN103190445 B CN 103190445B
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Abstract
To solve the problem that the sterilization range of the existing disinfectant is not applicable to article disinfection, the invention provides an acid washing sterilization disinfectant and a use method thereof, and belong to the technical field of composite sterilization disinfectants, wherein the sterilization disinfectant comprises 0.2-1mol.L<-1> hydrochloric acid, potassium permanganate and sodium thiosulfate (the mole ratio of HCl in the hydrochloric acid to the potassium permanganate and the sodium thiosulfate is 1:(0.0106-0.137):(0.027-0.312), and the sterilization disinfectant can be used for killing bacterium, virus, rickettsia, chlamydia, mycoplasma, spirochete, actinomycetes and as well as fungus, mold, parasite and the like. The acid washing sterilization disinfectant integrates the functions of disinfection, sterilization and whitening, furthermore dirty in seams can be washed, enhanced composite sterilization effects are achieved, the sterilization range is increased, and pathogen can be sterilized, which is impossible for a single sanitizer; and the sterilization disinfectant can be used for washing public urinals in hospitals after being used, so that the sterilization disinfectant can be both used for sterilizing and washing urine dirty and the like.
Description
Technical field
The present invention relates to a kind of acid washing and sterilizing disinfectant and using method thereof, belong to the technical field of composite sterilization disinfectant.
Background technology
Because hospital's hand brush, all kinds of hairbrush gap residues inside are difficult, remove easy breed bacteria and mould, residue does not wash clean clearly not only not attractive in appearance, and disinfection thoroughly not have hidden danger be a current difficult problem.If have again, have in the middle of test tube for blood sample frame the utensils such as washbowl that residual bloodstain hairbrush is difficult to brush, lazaretto ward patient uses, Sputum cup (broad-mouthed receptacle for holding liquid), chamber pot, toilet have urine dirt and smell large, do not sterilize and easily propagate the infectious diseases such as skin disease, venereal disease, hepatitis, so need especially to remove gap dirt, again can wide spectrum kill bacteria (Gram-positive and Gram-negative bacteria), pathogene, the parasitic disinfectant such as fungi, virus, gonococcus, mould, trichomonad, microspironema pallidum, this invention disinfectant all can meet requirements.Virus, bacterium, fungi, parasite etc. are all the arch-criminals who causes infectious disease, have public object each hospital, hotel, hotel, receive family in one's power and all will use in a large number, according to statistics because floating population increases, the infectious disease incidences such as current venereal disease, skin disease, hepatitis have the tendency increasing, so resolve the sterilization problems of public place, the propagation of controlling infectious disease is the social task of top priority.
Summary of the invention
But; The sterilizing scope of existing each disinfectant has the limitation that is not suitable for sterilizing objects.In order to address this problem, the invention provides a kind of can kill bacteria, the acid washing and sterilizing disinfectant of virus, rickettsia, Chlamydia, mycoplasma, conveyor screw, actinomycetes and fungi, mould, parasite etc.There is wide spectrum, sterilized (can kill vegetative forms of bacteria, the various microorganisms such as fungi, virus and bacterial spore), little to environmental influence, economic environmental protection.
The present invention also provides the using method of this acidity washing and sterilizing disinfectant.
Technical scheme of the present invention:
An acid washing and sterilizing disinfectant, by concentration 0.2-1 molL
-1hydrochloric acid, potassium permanganate and sodium thiosulfate form; In hydrochloric acid, the mol ratio of HCl, potassium permanganate, sodium thiosulfate is 1:0.0106-0.137:0.027-0.312.
Above-mentioned acid washing and sterilizing disinfectant, the concentration of hydrochloric acid is preferably 0.6024mol/L.
Above-mentioned acid washing and sterilizing disinfectant, in hydrochloric acid, HCl, potassium permanganate, the preferred mol ratio of sodium thiosulfate are 1:0.0315:0.064.
A using method for above-mentioned acid washing and sterilizing disinfectant, comprises the steps:
First, need article to be processed are placed in to hydrochloric acid, soak 10-30min;
Then, add potassium permanganate and make it dissolve rear standing 5-45min completely;
Finally, then add sodium thiosulfate and make it dissolve rear standing 15-50min completely.
Described article are non-metal article, alloy and in the periodic table of elements, come the metal (as copper, platinum, gold) after hydrogen.
The using method of above-mentioned acid washing and sterilizing disinfectant,
The soak time of step (1) is preferably 15-20min, more preferably 15min;
The time of repose of step (2) is preferably 15-20min, is more preferably 20min;
The time of repose of step (3) is preferably 25-30min, is more preferably 30min.
The using method of above-mentioned acid washing and sterilizing disinfectant, preferred, step (1) (2) (3) is all carried out under closed environment; More preferably adopt plastic box with lid as container.
The using method of above-mentioned acid washing and sterilizing disinfectant, preferably adopts the method with oscillator concussion that potassium permanganate and sodium thiosulfate are dissolved.
The using method of above-mentioned acid washing and sterilizing disinfectant, for the more article of grease, preferred, in step (1) before, first use 0.1% acid degreasing agent (BG-CLEANER) article-cleaning.
The using method of above-mentioned acid washing and sterilizing disinfectant, preferred, after being completed, step (3) filters to obtain treatment fluid; And treatment fluid is used for cleaning incrustation scale, urine dirt and tea stain; Preferred, with processing the soaked dirt of immersion, urine dirt and tea stain 5-15min.
On sterilizing objects during microbial contamination especially severe, during article that sterilization is polluted by blood, fester etc., need proper extension action time.
Acid washing and sterilizing disinfectant of the present invention, watery hydrochloric acid can kill part bacterium and virus in the process of soaking article; And potassium permanganate chance organic matter emits the effect that nascent oxygen has kill bacteria, under hydrochloric acid effect, discharge chlorine, further the pathogene such as bacterial propagule, virus, fungi, tubercle bacillus and bacterial spore, conveyor screw are killed in sterilization, sterilizing bleaching; And then generate the sulphur effect of a step disinfection again with sodium thiosulfate effect, also produce sulphur dioxide and the sulphur (hairbrush easily produces mould and fungi, and sulphur dioxide and sulphur are to its special efficacy) that has bleaching and antisepsis simultaneously.The present invention, by limiting wherein consumption and the concentration of each component, makes each component when interacting, both can bring into play self sterilization, disinfection efficacy; The material that possesses sterilization, sterilization, bleaching effect of release new can interact again.
Acid washing and sterilizing disinfectant of the present invention, is usually used in the sterilization to the utensil of the article such as all kinds of hairbrush of hospital, plastic test tube frame, enamel chamber pot, enamel, ceramic toilet and contagious ward.Also can inner wrapping for family disinfection toothbrush and family, have epidemic victim's article, with after clean again toilet urinal.Can meet preparation 0.5L, 1L, 5L, 10L, the acid washing and sterilizing disinfectant supply of 50L market according to the amount of user's need to design a package finished product hydrochloric acid, potassium permanganate and sodium thiosulfate.
Beneficial effect of the present invention:
(1) integrate the effect of sterilization, sterilizing, bleaching; Can also clean the dirt in gap;
(2) possessing several bactericidal effects that repeat to close, sterilizing scope is increased, can kill pathogene, is that independent disinfectant is not available;
(3) use and can be used for cleaning the public urinal of hospital afterwards, can sterilize, can clean up again urine dirt etc.; Can be used as the lavatory agent of sterilizing high-efficient cleaning.
Embodiment
Embodiment 1
Experimental group: put in 500ml beaker and scribble respectively in advance staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, staphylococcus albus, bacillus subtilis black variety gemma, Mycobacterium chelonei abscess subspecies, Candida albicans and black-koji mould bacterial classification, bacteria containing amount is 5 * 10
5cfu/ sheet～5 * 10
68 10mm * 10mm slides of cfu/ sheet, and prior 3 10mm * 10mm slides that scribble respectively polyovirus-I type vaccine strain, hepatitis B, AIDS virus serum, add 100ml, 0.6024 molL
-1concentrated hydrochloric acid, then add 0.1012gKMnO
4stirring is dissolved it completely, adds 0.4030gNa after 15min
2s
2o
3, stir it dissolved completely.After 30min, take out after each slide water cleans and check.
Quantitative positive controls: to put under room temperature with bacterium sheet with batch test, to be sterilized or sterile test group reaches regulation after action time, immediately 2 of this bacterium sheets is put into respectively to the test tube containing 5.0ml PBS, respectively shakes and strikes 80 times.Get washing lotion and carry out viable bacteria cultivation counting by the < < disinfection technology standard > > viable bacteria method shown in counting technology of cultivating.
Qualitative positive controls: to put under room temperature with bacterium sheet with batch test, experimental group subject to sterilization reaches regulation after action time, by 2 of this bacterium sheets, is inoculated in respectively 5.0ml nutrient broth medium immediately, put into incubator and make Qualitative culture, observe and have bacterial growth situation.
Negative control group: with 2 of microbiological contamination prints not for batch test, be inoculated in respectively 5.0ml nutrient broth medium, the nutrient broth medium of not inoculating put into incubator simultaneously and make Qualitative culture, observe and have or not bacterial growth.
Above-mentioned sterile test all in triplicate.
All experimental group slides are all without bacterial growth, and the kill logarithm value scope 3.36-3.83 of 3 tests to each quasi-microorganism, can be judged to sterilizing qualified.Infection titer (TCID according to 3 each average viruses of organizing
50), calculate respectively it to viral deactivation logarithm value scope 4.21-4.63.This acidity washing and sterilizing disinfectant has reached the effect of the sterilizing of killing the virus.
Through 3 sterile tests, the quantitative positive controls in each test, detects recovery bacterium amount and all reaches 5 * 10
5～5 * 10
6cfu/ sheet; Qualitative positive controls, bacterial growth is good.Negative control asepsis growth.
Embodiment 2
Experimental group: put in 500ml beaker and scribble respectively in advance staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, staphylococcus albus, bacillus subtilis black variety gemma, Mycobacterium chelonei abscess subspecies, Candida albicans and black-koji mould bacterial classification, bacteria containing amount is 5 * 10
5cfu/ sheet～5 * 10
68 10mm * 10mm slides of cfu/ sheet, and prior 3 10mm * 10mm slides that scribble respectively polyovirus-I type vaccine strain, hepatitis B, AIDS virus serum, add 100ml, 0.6024 molL
-1concentrated hydrochloric acid, then add 0.2011gKMnO
4stirring is dissolved it completely, adds 0.7090gNa after 10min
2s
2o
3, stir it dissolved completely.After 25min, take out after each slide water cleans and check.
Quantitatively positive controls, puts under room temperature with bacterium sheet with same batch of test, and to be sterilized or sterile test group reaches regulation after action time, immediately 2 of this bacterium sheets is put into respectively to the test tube containing 5.0ml PBS, respectively shakes and strikes 80 times.Get washing lotion and carry out viable bacteria cultivation counting by the < < disinfection technology standard > > viable bacteria method shown in counting technology of cultivating.
Qualitative positive controls, to put under room temperature with bacterium sheet with batch test, experimental group subject to sterilization reaches regulation after action time, immediately by 2 of this bacterium sheets, be inoculated in respectively 5.0ml nutrient broth medium, put into incubator and make Qualitative culture, observe and have bacterial growth situation.
Negative control group, with 2 of microbiological contamination prints not for batch test, is inoculated in respectively 5.0ml nutrient broth medium, the nutrient broth medium of not inoculating is put into incubator simultaneously and makes Qualitative culture, observes and has or not bacterial growth.
Above-mentioned sterile test all in triplicate.
All experimental group slides are all without bacterial growth, and the kill logarithm value scope 3.62-4.27 of 3 tests to each quasi-microorganism, can be judged to sterilizing qualified.Infection titer (TCID according to 3 each average viruses of organizing
50), calculate respectively it to viral deactivation logarithm value scope 4.43-4.71.This acidity washing and sterilizing disinfectant has reached the effect of the sterilizing of killing the virus.
Quantitative positive controls in each test, detects recovery bacterium amount and all reaches 5 * 10
5～5 * 10
6cfu/ sheet; Qualitative positive controls, bacterial growth is good.Negative control asepsis growth.
Embodiment 3
Experimental group: put in 500ml beaker and scribble respectively in advance staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, staphylococcus albus, bacillus subtilis black variety gemma, Mycobacterium chelonei abscess subspecies, Candida albicans and black-koji mould bacterial classification, bacteria containing amount is 5 * 10
5cfu/ sheet～5 * 10
68 10mm * 10mm slides of cfu/ sheet, and prior 3 10mm * 10mm slides that scribble respectively polyovirus-I type vaccine strain, hepatitis B, AIDS virus serum, add 100ml, 0.6024 molL
-1concentrated hydrochloric acid, then add 0.3017gKMnO
4stirring is dissolved it completely, adds 0.9585gNa after 10min
2s
2o
3, stir it dissolved completely.After 20min, take out after each slide water cleans and check.
Quantitatively positive controls, puts under room temperature with bacterium sheet with same batch of test, and to be sterilized or sterile test group reaches regulation after action time, immediately 2 of this bacterium sheets is put into respectively to the test tube containing 5.0ml PBS, respectively shakes and strikes 80 times.Get washing lotion and carry out viable bacteria cultivation counting by the < < disinfection technology standard > > viable bacteria method shown in counting technology of cultivating.
Qualitative positive controls, to put under room temperature with bacterium sheet with batch test, experimental group subject to sterilization reaches regulation after action time, immediately by 2 of this bacterium sheets, be inoculated in respectively 5.0ml nutrient broth medium, put into incubator and make Qualitative culture, observe and have bacterial growth situation.
Negative control group, with 2 of microbiological contamination prints not for batch test, is inoculated in respectively 5.0ml nutrient broth medium, the nutrient broth medium of not inoculating is put into incubator simultaneously and makes Qualitative culture, observes and has or not bacterial growth.
Above-mentioned sterile test all in triplicate.
All experimental group slides are all without bacterial growth, and the kill logarithm value scope 3.95-4.52 of 3 tests to each quasi-microorganism, can be judged to sterilizing qualified.Infection titer (TCID according to 3 each average viruses of organizing
50), calculate respectively it to viral deactivation logarithm value scope 4.50-4.83.This acidity washing and sterilizing disinfectant has reached the effect of the sterilizing of killing the virus.
Quantitative positive controls in each test, detects recovery bacterium amount and all reaches 5 * 10
5～5 * 10
6cfu/ sheet; Qualitative positive controls, bacterial growth is good.Negative control asepsis growth.
Embodiment 4
Experimental group: put in 500ml beaker and scribble respectively in advance staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, staphylococcus albus, bacillus subtilis black variety gemma, Mycobacterium chelonei abscess subspecies, Candida albicans and black-koji mould bacterial classification, bacteria containing amount is 5 * 10
5cfu/ sheet～5 * 10
68 10mm * 10mm slides of cfu/ sheet, and prior 3 10mm * 10mm slides that scribble respectively polyovirus-I type vaccine strain, hepatitis B, AIDS virus serum, add 100ml, 0.2molL
-1concentrated hydrochloric acid, then add 0.4318gKMnO
4stirring is dissolved it completely, adds 1.5453gNa after 20min
2s
2o
3, stir it dissolved completely.After 30min, take out after each slide water cleans and check.
Quantitatively positive controls, puts under room temperature with bacterium sheet with same batch of test, and to be sterilized or sterile test group reaches regulation after action time, immediately 2 of this bacterium sheets is put into respectively to the test tube containing 5.0ml PBS, respectively shakes and strikes 80 times.Get washing lotion and carry out viable bacteria cultivation counting by the < < disinfection technology standard > > viable bacteria method shown in counting technology of cultivating.
Qualitative positive controls, to put under room temperature with bacterium sheet with batch test, experimental group subject to sterilization reaches regulation after action time, immediately by 2 of this bacterium sheets, be inoculated in respectively 5.0ml nutrient broth medium, put into incubator and make Qualitative culture, observe and have bacterial growth situation.
Negative control group, with 2 of microbiological contamination prints not for batch test, is inoculated in respectively 5.0ml nutrient broth medium, the nutrient broth medium of not inoculating is put into incubator simultaneously and makes Qualitative culture, observes and has or not bacterial growth.
Above-mentioned sterile test all in triplicate.
All experimental group slides are all without bacterial growth, and the kill logarithm value scope 3.16-3.43 of 3 tests to each quasi-microorganism, can be judged to sterilizing qualified.Infection titer (TCID according to 3 each average viruses of organizing
50), calculate respectively it to viral deactivation logarithm value scope 4.08-4.23.This acidity washing and sterilizing disinfectant has reached the effect of the sterilizing of killing the virus.
Quantitative positive controls in each test, detects recovery bacterium amount and all reaches 5 * 10
5～5 * 10
6cfu/ sheet; Qualitative positive controls, bacterial growth is good.Negative control asepsis growth.
Embodiment 5
Experimental group: put in 500ml beaker and scribble respectively in advance staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, staphylococcus albus, bacillus subtilis black variety gemma, Mycobacterium chelonei abscess subspecies, Candida albicans and black-koji mould bacterial classification, bacteria containing amount is 5 * 10
5cfu/ sheet～5 * 10
68 10mm * 10mm slides of cfu/ sheet, and prior 3 10mm * 10mm slides that scribble respectively polyovirus-I type vaccine strain, hepatitis B, AIDS virus serum, add 100ml, 1.0molL
-1concentrated hydrochloric acid, then add 0.4753gKMnO
4stirring is dissolved it completely, adds 1.655gNa after 20min
2s
2o
3, stir it dissolved completely.After 30min, take out after each slide water cleans and check.
Quantitative positive controls: to put under room temperature with bacterium sheet with batch test, to be sterilized or sterile test group reaches regulation after action time, immediately 2 of this bacterium sheets is put into respectively to the test tube containing 5.0ml PBS, respectively shakes and strikes 80 times.Get washing lotion and carry out viable bacteria cultivation counting by the < < disinfection technology standard > > viable bacteria method shown in counting technology of cultivating.
Qualitative positive controls: to put under room temperature with bacterium sheet with batch test, experimental group subject to sterilization reaches regulation after action time, by 2 of this bacterium sheets, is inoculated in respectively 5.0ml nutrient broth medium immediately, put into incubator and make Qualitative culture, observe and have bacterial growth situation.
Negative control group: with 2 of microbiological contamination prints not for batch test, be inoculated in respectively 5.0ml nutrient broth medium, the nutrient broth medium of not inoculating put into incubator simultaneously and make Qualitative culture, observe and have or not bacterial growth.
Above-mentioned sterile test all in triplicate.
All experimental group slides are all without bacterial growth, and the kill logarithm value scope 4.25-4.82 of 3 tests to each quasi-microorganism, can be judged to sterilizing qualified.Infection titer (TCID according to 3 each average viruses of organizing
50), calculate respectively it to viral deactivation logarithm value scope 4.67-4.98.This acidity washing and sterilizing disinfectant has reached the effect of the sterilizing of killing the virus.
Quantitative positive controls in each test, detects recovery bacterium amount and all reaches 5 * 10
5～5 * 10
6cfu/ sheet; Qualitative positive controls, bacterial growth is good.Negative control asepsis growth.
In the bactericidal assay process of embodiment 1-5, first the hydrochloric acid of antibacterial disinfectant makes PH < 1, can kill the virus and bacterium; Add potassium permanganate and be strong oxidizer and meet organic matter and release new ecological oxygen, can sterilization, sterilization, and have astriction; In course of reaction, all can produce chlorine, sulphur dioxide and sulphur, thereby play the effect that promotes sterilizing; By controlling the ratio of HCl, potassium permanganate, sodium thiosulfate, make the concentration of chlorine, sulphur dioxide and the sulphur of generation be not enough to environment to exert an influence simultaneously.
Embodiment 6
Prepare the plastics disinfect box with lid of a 5L; The non-metal articles such as the various hairbrush of needs sterilization, rack for test tube are placed in disinfect box.In disinfect box, add 3L watery hydrochloric acid (0.6 molL
-1), make hydrochloric acid not have the article in disinfect box completely; Cover lid soaks 15min; Then add 3gKMnO
4, stir and make KMnO
4dissolve completely, cover lid soaks 20min; Add again 12gNa
2s
2o
3, stir and make to stir Na
2s
2o
3dissolve completely, cover lid soaks 30min.Take out the article such as hairbrush, rack for test tube and detect, reach sterilization standard.Residue treatment fluid in disinfect box is poured into urinating in dirty toilet, soaked after 15min, use clear water flushing closestool, urine dirt disappears.
Embodiment 7
Prepare the plastics disinfect box with lid of a 10L; The various glass wares that needs are sterilized etc. are placed in disinfect box.In disinfect box, add 8L watery hydrochloric acid (0.6 molL
-1), make hydrochloric acid not have the article in disinfect box completely; Cover lid soaks 1min; Then add 20gKMnO
4, stir and make KMnO
4dissolve completely, cover lid soaks 5min; Add again 70gNa
2s
2o
3, stir and make to stir Na
2s
2o
3dissolve completely, cover lid soaks 15min.Take out the article such as hairbrush, rack for test tube and detect, reach sterilization standard.Residue treatment fluid in disinfect box is poured into urinating in dirty toilet, soaked after 5min, use clear water flushing closestool, urine dirt disappears.
Embodiment 8
Prepare the plastics disinfect box with lid of a 50L; In disinfect box, add 40L watery hydrochloric acid (0.6molL
-1), make hydrochloric acid not have hairbrush, rack for test tube etc. completely; Cover lid soaks 15min; Then add 80gKMnO
4, stir and make KMnO
4dissolve completely, cover lid soaks 20min; Add again 280gNa
2s
2o
3, stir and make Na
2s
2o
3dissolve completely, cover lid soaks 30min.Take out the article such as hairbrush, rack for test tube and detect, reach sterilization standard.Patient's the clothes, sheet, quilt cover etc. of need to sterilizing are placed on cover lid in disinfect box and soak 30min, also can reach sterilization standard.Residue treatment fluid in disinfect box is soaked to rag 30min, then put on mouth mask, rubber gloves, put on rubber boot, with rag wiping table top article, liquid is poured Plastic Drum into and is rinsed and wash mop, then drags toilet floor surface with wet mop.The liquid gradation being finally finished is poured into urinating in dirty toilet, soaks after 5min, uses clear water flushing closestool, and urine dirt disappears.
Claims (8)
1. a using method for acid washing and sterilizing disinfectant, is characterized in that, by concentration 0.2-1 molL
-1hydrochloric acid, potassium permanganate and sodium thiosulfate form; In hydrochloric acid, the mol ratio of HCl, potassium permanganate, sodium thiosulfate is 1:0.0106-0.137:0.027-0.312;
Its using method comprises the steps:
First, need article to be processed are placed in to hydrochloric acid, soak 10-30min;
Then, add potassium permanganate and make it dissolve rear standing 5-45min completely;
Finally, then add sodium thiosulfate and make it dissolve rear standing 15-50min completely.
2. according to right, want the using method of the acid washing and sterilizing disinfectant described in 1, it is characterized in that, the concentration of hydrochloric acid is 0.6024mol/L.
3. according to right, want the using method of the acid washing and sterilizing disinfectant described in 1 or 2, it is characterized in that, in hydrochloric acid, the mol ratio of HCl, potassium permanganate, sodium thiosulfate is 1:0.0315:0.064.
4. the using method of acid washing and sterilizing disinfectant according to claim 1, is characterized in that,
The soak time of step (1) is 15-20min;
The time of repose of step (2) is 15-20min;
The time of repose of step (3) is 25-30min.
5. the using method of acid washing and sterilizing disinfectant according to claim 1, is characterized in that,
The soak time of step (1) is 15min;
The time of repose of step (2) is 20min;
The time of repose of step (3) is 30min.
6. the using method of acid washing and sterilizing disinfectant according to claim 1, is characterized in that,
Step (1) (2) (3) is all carried out under closed environment.
7. the using method of acid washing and sterilizing disinfectant according to claim 1, is characterized in that, adopts the method with oscillator concussion that potassium permanganate and sodium thiosulfate are dissolved.
8. the using method of acid washing and sterilizing disinfectant according to claim 4, is characterized in that, for the more article of grease, in step (1) before, first uses 0.1% acid degreasing agent BG-CLEANER article-cleaning.
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| CN201310133017.XA CN103190445B (en) | 2013-04-17 | 2013-04-17 | Acid washing sterilization disinfectant and use method thereof |
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|---|---|---|---|
| CN201310133017.XA CN103190445B (en) | 2013-04-17 | 2013-04-17 | Acid washing sterilization disinfectant and use method thereof |
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| CN103190445B true CN103190445B (en) | 2014-10-08 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105053004A (en) * | 2015-08-11 | 2015-11-18 | 吕晨兴 | Potassium and sulfur mixture, preparation method therefor and application thereof |
| CN105340963A (en) * | 2015-11-30 | 2016-02-24 | 芜湖成德龙过滤设备有限公司 | Casing sterilizing solution material composition, and preparation method and application thereof |
| CN113699375A (en) * | 2021-09-01 | 2021-11-26 | 河北工程大学 | Additive solution for improving leaching rate of rare earth elements leached by microorganisms, preparation method and application |
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|---|---|---|---|---|
| CN1454504A (en) * | 2002-04-30 | 2003-11-12 | 宋述孝 | Freeze-dried vegetable preparing method |
| CN1771990A (en) * | 2005-11-11 | 2006-05-17 | 李柳川 | Specific recipe for killing skin fungus |
| CN101036447A (en) * | 2007-04-28 | 2007-09-19 | 大连水产学院 | Seawater domestication and indoor high-density culture method of moina mongolica daday |
| CN101353209A (en) * | 2007-07-25 | 2009-01-28 | 史梅约 | Sectional type water purification treatment agent |
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2013
- 2013-04-17 CN CN201310133017.XA patent/CN103190445B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1454504A (en) * | 2002-04-30 | 2003-11-12 | 宋述孝 | Freeze-dried vegetable preparing method |
| CN1771990A (en) * | 2005-11-11 | 2006-05-17 | 李柳川 | Specific recipe for killing skin fungus |
| CN101036447A (en) * | 2007-04-28 | 2007-09-19 | 大连水产学院 | Seawater domestication and indoor high-density culture method of moina mongolica daday |
| CN101353209A (en) * | 2007-07-25 | 2009-01-28 | 史梅约 | Sectional type water purification treatment agent |
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