CN103172437A - Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof - Google Patents
Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof Download PDFInfo
- Publication number
- CN103172437A CN103172437A CN2012105924469A CN201210592446A CN103172437A CN 103172437 A CN103172437 A CN 103172437A CN 2012105924469 A CN2012105924469 A CN 2012105924469A CN 201210592446 A CN201210592446 A CN 201210592446A CN 103172437 A CN103172437 A CN 103172437A
- Authority
- CN
- China
- Prior art keywords
- polyglutamic acid
- liquid
- parts
- fertilizer
- composite microbiological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003337 fertilizer Substances 0.000 title claims abstract description 55
- 239000007788 liquid Substances 0.000 title claims abstract description 49
- 229920002643 polyglutamic acid Polymers 0.000 title claims abstract description 41
- 108010020346 Polyglutamic Acid Proteins 0.000 title claims abstract description 27
- 150000001875 compounds Chemical class 0.000 title claims abstract description 26
- 230000000813 microbial effect Effects 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000015097 nutrients Nutrition 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 12
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 11
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 10
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 10
- 239000005416 organic matter Substances 0.000 claims abstract description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 4
- 239000011574 phosphorus Substances 0.000 claims abstract description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000002131 composite material Substances 0.000 claims description 28
- 239000002689 soil Substances 0.000 claims description 23
- 230000002906 microbiologic effect Effects 0.000 claims description 22
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 16
- 230000001580 bacterial effect Effects 0.000 claims description 15
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 14
- 229940024606 amino acid Drugs 0.000 claims description 11
- 235000001014 amino acid Nutrition 0.000 claims description 11
- 150000001413 amino acids Chemical class 0.000 claims description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 10
- 239000011591 potassium Substances 0.000 claims description 10
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 229960002989 glutamic acid Drugs 0.000 claims description 7
- 229930182817 methionine Natural products 0.000 claims description 7
- 235000007686 potassium Nutrition 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- 239000004475 Arginine Substances 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 208000006558 Dental Calculus Diseases 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 5
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 5
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 5
- 241001668545 Pascopyrum Species 0.000 claims description 5
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 5
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 5
- 235000003704 aspartic acid Nutrition 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000004021 humic acid Substances 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 235000013619 trace mineral Nutrition 0.000 claims description 5
- 239000011573 trace mineral Substances 0.000 claims description 5
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 239000005696 Diammonium phosphate Substances 0.000 claims description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 2
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 claims description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000004473 Threonine Substances 0.000 claims description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 2
- 239000006035 Tryptophane Substances 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 235000013877 carbamide Nutrition 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 2
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 2
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 2
- 230000005059 dormancy Effects 0.000 claims description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
- 239000006012 monoammonium phosphate Substances 0.000 claims description 2
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 2
- 229940023462 paste product Drugs 0.000 claims description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 2
- 235000015320 potassium carbonate Nutrition 0.000 claims description 2
- 239000004323 potassium nitrate Substances 0.000 claims description 2
- 235000010333 potassium nitrate Nutrition 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 229960004799 tryptophan Drugs 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims 2
- 241000238631 Hexapoda Species 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 230000000593 degrading effect Effects 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 15
- 108700022290 poly(gamma-glutamic acid) Proteins 0.000 description 12
- 210000003608 fece Anatomy 0.000 description 9
- 239000010871 livestock manure Substances 0.000 description 9
- 235000010469 Glycine max Nutrition 0.000 description 6
- 241000209140 Triticum Species 0.000 description 6
- 235000021307 Triticum Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000002791 soaking Methods 0.000 description 5
- 230000035558 fertility Effects 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 235000014304 histidine Nutrition 0.000 description 4
- 235000001055 magnesium Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000008729 phenylalanine Nutrition 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- -1 phosphorus compound Chemical class 0.000 description 4
- 235000004400 serine Nutrition 0.000 description 4
- 235000008521 threonine Nutrition 0.000 description 4
- 235000017103 tryptophane Nutrition 0.000 description 4
- 235000002374 tyrosine Nutrition 0.000 description 4
- 235000016804 zinc Nutrition 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 3
- 241001124569 Lycaenidae Species 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 241000219793 Trifolium Species 0.000 description 3
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Chemical class CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 150000001510 aspartic acids Chemical class 0.000 description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 235000014987 copper Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 3
- 150000002411 histidines Chemical class 0.000 description 3
- 235000000396 iron Nutrition 0.000 description 3
- 235000002908 manganese Nutrition 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 229940066779 peptones Drugs 0.000 description 3
- 150000002994 phenylalanines Chemical class 0.000 description 3
- 235000019353 potassium silicate Nutrition 0.000 description 3
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 3
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 150000003588 threonines Chemical class 0.000 description 3
- 150000003654 tryptophanes Chemical class 0.000 description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 3
- 229960001763 zinc sulfate Drugs 0.000 description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
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- 239000002253 acid Substances 0.000 description 2
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Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a liquid compound microbial fertilizer containing polyglutamic acid, and a preparation method and an application thereof, and belongs to the field of compound microbial fertilizers. Bacillus megaterium, bacillus thuringiensis and bacillus amyloliquefaciens are prepared into a bacteria liquid by liquid fermentation, and then the bacteria liquid is mixed with compound nutrients and a gamma-polyglutamic acid according to the mass ratio of 1:1:0.05-0.1 to be prepared into the efficient liquid compound microbial fertilizer. On the basis of good phosphorus dissolving capability of the bacillus megaterium, good insect resistance of the bacillus amyloliquefaciens and good organic matter degrading capability of the bacillus amyloliquefaciens, the purposes of more excellent fertilizer efficiency and lower water consumption are achieved under the characteristics of water retention, fertilizer preservation, and no environmental pollution of the gamma-polyglutamic acid, therefore, the scientific and reasonable matching of the compound fertilizers is formed.
Description
Technical field
The invention belongs to the composite microbic bacterial fertilizer field, be specifically related to a kind of liquid composite microbiological fertilizer that contains polyglutamic acid and its preparation method and application.
Background technology
Clearly advocate in ten large countermeasures of China's ecological environmental protection and will promote " ecological agriculture "; preserve the ecological environment and agricultural land soil; promote the production of pollution-free healthy food; ensure the sufficient amount organic matter and return to soil; to promote biological cycle in the ecosystem, reach maintenance and strengthen soil fertility and biological activity purpose.Microbiobacterial agent can play and prevent disease and nontoxic to people and animals, is the preferred production means of high-quality Agricultural product without pollution.Fast development along with China's modern agriculture, particularly the green organic agricultural cultivated area constantly enlarges, the specified microorganisms bacterium is dissolved into fertilizer, and the fertilizer product of producing and complete function useful to soil becomes the emphasis of current fertiliser production enterprise development.
After at present the field entered soil to the excessive organic/inorganic fertilizer of crop applying, its residue accumulated worked the mischief to ecological environment of soil to a certain extent the time.Be base manure and composite microbiological fertilizer is a kind of composite fertilizer, be rich in the novel non-pollution bio-feritlizer of several functions bacterium.Function yeast wherein can be regulated the balance of soil flora, has the improvement Soil structure, increases soil fertility and output level, improves the effects such as plant quality.Especially this product has added gamma-polyglutamic acid-, has better water conservation, fertilizer conservation effect.
Research and development for composite microbic bacterial fertilizer have at present become trend, and are also of common occurrence about the patent of composite microbic bacterial fertilizer in recent years, but the invention in conjunction with polyglutamic acid and composite microbic bacterial fertilizer not yet occurs.The present invention adds tall and erect effect composition gamma-polyglutamic acid-on the basis of composite biological fertilizer, its remarkable water conservation, province's fertilizer, biological degradability make better effect of microbial fertilizer performance, as increasing production, economize on water, economize fertilizer, non-environmental-pollution, adjusting soil pH etc.
(γ-PGA) is the extracellular anionic polyamino acid that the occurring in nature microbial fermentation produces to gamma-polyglutamic acid-, by the high molecular polymer that forms with the amido linkage form by α amino and γ-carboxyl between D type L-glutamic acid, L-type L-glutamic acid monomer.γ-PGA is biodegradable, good water absorption and have good biocompatibility.
Thereby γ-PGA has the plant growth of promotion on agricultural improves the potential function of crop yield.Find in the germination test of γ-PGA seed soaking, bud length, root length, germination factor, amylase activity and the activity of catalase etc. of Plantula Brassicae chinensis, wheat, corn, cotton etc. all have raising in various degree, illustrate that γ-PGA can improve seed vitality.Proved that also γ-PGA can improve the output of Plantula Brassicae chinensis, amplification can reach more than 30%, and the amplitude that improves output when fertilizer is poorer is larger, has significant premium synergism.γ-PGA tests the seed soaking of tobacco seed and also shows, γ-PGA can significantly improve the activity of root length, bud length and the various enzymes of tobacco.
Summary of the invention
The purpose of this invention is to provide a kind of liquid composite microbiological fertilizer that contains polyglutamic acid and preparation method thereof, composite microbiological fertilizer provided by the invention is take the organic and inorganic compound nutrients as base manure, add types of functionality microorganism and gamma-polyglutamic acid-, thereby effectively increase soil fertility, improve crop growth environment.
Another object of the present invention is that the liquid composite microbiological fertilizer that contains polyglutamic acid of the present invention is applied in soil application.
Technical solution of the present invention is as follows:
The described composition that contains the liquid composite microbiological fertilizer of polyglutamic acid comprises microbial fermentation bacterium liquid, compound nutrients, gamma-polyglutamic acid-;
Described microbial fermentation bacterium liquid: compound nutrients: the mass ratio of gamma-polyglutamic acid-is 1:1:0.05-0.1;
Consisting of of described microbial fermentation bacterium liquid: the fermented liquid of bacillus thuringiensis (Bacillus thuringiensis), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), three kinds of bacterial strains of bacillus megaterium (Bacillus megaterium) mixes with the mass ratio of 1:1:1;
Described compound nutrients comprises nitrogen, phosphorus, potassium element, trace element, organic matter, aminoacids complex, wheat grass meal;
Described nitrogen element is at least a in urea, ammonium sulfate, ammonium chloride;
Described phosphoric is at least a in monoammonium phosphate, diammonium phosphate, calcium superphosphate, potassium primary phosphate;
Described potassium element is at least a in vitriolate of tartar, Repone K, salt of wormwood, saltpetre;
Described trace element consists of: calcium, magnesium, silicon, zinc, manganese, boron, iron, copper, all add with the form of its soluble compound;
Described organic matter is humic acids or soluble proteins or both mixtures;
Consisting of of described aminoacids complex: L-Ala, arginine, aspartic acid, Gelucystine, L-glutamic acid, glycine, Histidine, Methionin, methionine(Met), phenylalanine, proline(Pro), Serine, Threonine, tryptophane, tyrosine, α-amino-isovaleric acid;
The preparation method of the described liquid composite microbiological fertilizer that contains polyglutamic acid is as follows:
(1) actication of culture
Bacillus thuringiensis, bacillus amyloliquefaciens, the activation of bacillus megaterium bacterial strain that 4 ℃ of inclined-planes are preserved, after with its respectively streak inoculation on LB solid plate substratum, 37 ℃, cultivate 24h;
(2) seed liquor preparation
The bacterium colony of picking activation is inoculated in respectively in the LB liquid nutrient medium, and 37 ℃, 180rpm are cultivated 20h;
(3) fermentation culture
Cultured seed liquor is seeded to fermention medium with 10% inoculum size respectively, 37 ℃, rotating speed 300rpm, ventilating ratio 1:1, collecting zymocyte liquid after fermentation 20h is liquid bacterial fertilizer;
Described fermention medium consists of: by percentage to the quality, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium primary phosphate, 0.15% urea, 0.5% yeast powder, 0.41 ‰ sal epsom, all the other are water, pH 7.0-7.2;
(4) diatomite is fixed
The diatomite that adds mass ratio 10% in the zymocyte liquid that step (3) is collected is with fixing thalline and promote the thalline dormancy;
(5) add compound nutrients and polyglutamic acid
Three kinds of bacterium liquid of above-mentioned steps (4) mass ratio with 1:1:1 is mixed, compound nutrients is proportionally added mixing, proportionally successively compound nutrients and gamma-polyglutamic acid-are added in the bacterium liquid that mixes, fully mixing;
(6) can
Above-mentioned mixing liquid is added in the Plastic Bottle of uv sterilisation, paste Product labelling, namely get composite microbiological fertilizer of the present invention.
The described composite microbiological fertilizer that contains polyglutamic acid can dilute after suitable multiple direct spraying in the kind of plant blade face or be directly used in seed soaking, also can be applicable in soil application.
Beneficial effect:
The present invention is take the organic and inorganic compound nutrients as base manure, and base manure offers the enough nutriment of thalli growth, and nonpollution environment for balance fertilizing, improves utilization rate of fertilizer, promotes the stable high yield of crop that very important effect is arranged.Adding bacillus thuringiensis in this base manure can produce specific killing action to insect, respectively the various insects such as lepidopteran, Diptera, Coleoptera, Hymenoptera, Homoptera, Orthoptera, Mallophaga is had activity; Add bacillus megaterium and not only can decompose the organophosphorus material, and can decompose the insoluble phosphorus compound.Owing to producing a large amount of nitrite reductases, make it have stronger nitrite degradation ability; The bacillus amyloliquefaciens degradable starch for the plants and soil microorganism provides nutrient and movable energy, and affects soil property directly or indirectly.The nutritive substance of Substance Transformation for being absorbed and used that the vital movement of utilizing microorganism can not directly be utilized some crops in soil, improve the crop production excitor substance, the vigor of Suppressing phytopathogens, thus increase soil fertility, improve the crop alimentary condition, improve output.And added gamma-polyglutamic acid-, have remarkable ability for water conservation, fertilizer conservation, short giving birth to.
γ-PGA as fertilizer sources synergistic agent has and promotes farm crop to the absorption of nutrition, saves fertile synergy, and increasing yield and improving quality strengthens the effects such as crop disease-resistant ability, and fertilizer is had good slow releasing function, can reduce excessive or use the chemical fertilizer environmental pollution that causes such as improper.γ-PGA also has many electronegativies, can effectively stop the SO in chemical fertilizer
4 2-, CO
3 2-Plasma and Ca
2+, Mg
2+Combine Deng trace element, avoid producing the precipitation effect of low-solubility salt, make crop can more effectively absorb nutritive element in soil, promote crop root to grow, enhance disease resistance.For Pb
2+, Cu
2+Etc. heavy metal ion, γ-PGA has good chelating effect, can be used as heavy metals absorbent; In addition, acid, alkali are had surge capability preferably, but the pH-value of active balance soil is avoided the soil property acidifying and the soil compaction that cause because of life-time service chemical fertilizer.γ-PGA is the biotechnological formulation of pure natural, but natural decomposition generates amino acid, can not cause the secondary pollution to environment.Gamma-polyglutamic acid-also has splendid water-retentivity, the infusion of gamma-polyglutamic acid-has certain moisture holding capacity and comparatively ideal releasing effect in soil, there is obvious drought resisting to urge the seedling effect, can obviously improves the percentage of germination of wheat and rye grass, and can reduce the water cost.In sum, add polyglutamic acid can greatly improve effect of fertilizer in fertilizer.
Test shows, used that plant height, the number of blade, leaf that composite microbiological fertilizer of the present invention can significantly improve rape are long, leaf is wide, stem is thick and dry weight; Use its seed soaking can improve seedling rate; And due to water conservation, the water saving ability of gamma-polyglutamic acid-, can make irrigation water use save 10%, reduce cost.
Embodiment
Below by specific embodiment narration the present invention.Unless stated otherwise, in the present invention, technique means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Embodiment 1
The seed liquor of bacillus megaterium, bacillus thuringiensis, bacillus amyloliquefaciens is accessed respectively in fermention medium, 37 ℃, rotating speed 300rpm, ventilating ratio 1:1, fermentation 20h gets zymocyte liquid, adds diatomite and fixes, and three kinds of bacterium liquid mix with mass ratio 1:1:1; Described fermention medium consists of: by percentage to the quality, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium primary phosphate, 0.15% urea, 0.5% yeast powder, 0.41 ‰ sal epsom, all the other are water, pH 7.0-7.2;
The parts by weight of compound nutrients each component are: 8 parts, urea, 4 parts of monoammonium phosphates, 4 parts of vitriolate of tartar, 15 parts of chelating calcium, 7.5 parts, sal epsom, 7.5 parts of chelated magnesiums, 3 parts of water glass, 4 parts, zinc sulfate, 4 parts of chelated zincs, 2 parts of manganous sulfates, 2 parts of Chelated Manganeses, 8 parts of boric acid, 8 parts of chelated irons, 4 parts of chelated coppers, 0.5 part of humic acids, 5 parts of soy peptones, 3 parts of aminoacids complexs, 5 parts of wheat grass meals;
The parts by weight of described each component of aminoacids complex are: 5 parts of L-Ala, 10 parts of arginine, 5 parts of aspartic acids, 3 parts of Gelucystines, 2 parts, L-glutamic acid, 2 parts of glycine, 3 parts of Histidines, 10 parts of Methionins, 3 parts of methionine(Met), 5 parts of phenylalanines, 2 parts of proline(Pro), 1 part of Serine, 10 parts of Threonines, 5 parts of tryptophanes, 15 parts, tyrosine, 10 parts of α-amino-isovaleric acids;
Take bacterium liquid: compound nutrients: the mass ratio of gamma-polyglutamic acid-namely gets product 1 as the 1:1:0.1 mixing.
Described bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium bacterial classification are bought in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, 100101; Deposit number is respectively: CGMCC 1.792, and CGMCC 1.1226, and CGMCC 1.234, the time buying: in July, 2012.
Embodiment 2
The fermentation preparation of microbial inoculum is substantially with embodiment 1;
The parts by weight of each component of compound nutrients are: 8 parts, urea, 4 parts of monoammonium phosphates, 4 parts of vitriolate of tartar, 80 parts of potassium primary phosphates, 15 parts of chelating calcium, sal epsom 7.5,7.5 parts of chelated magnesiums, 3 parts of water glass, 4 parts, zinc sulfate, 4 parts of chelated zincs, 2 parts of manganous sulfates, 2 parts of Chelated Manganeses, 8 parts of boric acid, 8 parts of chelated irons, 4 parts of chelated coppers, 0.5 part of humic acids, 5 parts of soy peptones, 3 parts of aminoacids complexs, 5 parts of wheat grass meals;
The parts by weight of described each component of aminoacids complex are: 5 parts of L-Ala, 10 parts of arginine, 5 parts of aspartic acids, 3 parts of Gelucystines, 2 parts, L-glutamic acid, 2 parts of glycine, 3 parts of Histidines, 10 parts of Methionins, 3 parts of methionine(Met), 5 parts of phenylalanines, 2 parts of proline(Pro), 1 part of Serine, 10 parts of Threonines, 5 parts of tryptophanes, 15 parts, tyrosine, 10 parts of α-amino-isovaleric acids;
Take bacterium liquid: compound nutrients: the mass ratio of gamma-polyglutamic acid-namely gets product 2 as the 1:1:0.1 mixing.
Embodiment 3
The fermentation preparation of microbial inoculum is substantially with embodiment 1;
The parts by weight of each component of compound nutrients are: 88 parts, urea, 4 parts of monoammonium phosphates, 4 parts of vitriolate of tartar, 15 parts of chelating calcium, 7.5 parts, sal epsom, 7.5 parts of chelated magnesiums, 3 parts of water glass, 4 parts, zinc sulfate, 4 parts of chelated zincs, 2 parts of manganous sulfates, 2 parts of Chelated Manganeses, 8 parts of boric acid, 8 parts of chelated irons, 4 parts of chelated coppers, 0.5 part of humic acids, 5 parts of soy peptones, 3 parts of aminoacids complexs, 10 parts of wheat grass meals;
The parts by weight of described each component of aminoacids complex are: 5 parts of L-Ala, 10 parts of arginine, 5 parts of aspartic acids, 3 parts of Gelucystines, 2 parts, L-glutamic acid, 2 parts of glycine, 3 parts of Histidines, 10 parts of Methionins, 3 parts of methionine(Met), 5 parts of phenylalanines, 2 parts of proline(Pro), 1 part of Serine, 10 parts of Threonines, 5 parts of tryptophanes, 15 parts, tyrosine, 10 parts of α-amino-isovaleric acids;
Take bacterium liquid: compound nutrients: the mass ratio of gamma-polyglutamic acid-namely gets product 3 as the 1:1:0.05 mixing.
Embodiment 4
200 times of product 3 dilutions are sprayed in the rape blade face, spray a deuterzooid product every 7d, measure afterwards the rape indices the 80 days time of infertility and all be better than the not clear water control group of products applied 3, growing way is healthy and strong, presents blackish green.
Embodiment 5
200 times of product 1 dilutions are used for the wheat seed soaking, after plantation, compare with the control group that does not add gamma-polyglutamic acid-(other compositions and proportioning are all constant), the seedling rate that use has added the bacterial manure of polyglutamic acid to soak seed is higher than control group, illustrates to add polyglutamic acid that the effect that promotes wheat germination is arranged in bacterial manure.
Plant Height in Wheat, the root of using the product 1 bacterial manure processing of having added polyglutamic acid are long all greater than contrast, and the seedling color is dark, sturdy, well developed root system.Illustrate that the bacterial manure that adds polyglutamic acid is processed rear wheat growing way healthy and strong.
Embodiment 6
Corn, soybean, three kinds of crop seeds of trifolium in the dry ground condition are after planting executed product 2 when the whole ground and are watered, and not add gamma-polyglutamic acid-(other compositions and proportioning are all constant) as contrast, measure height of seedling.Result shows, used the soybean of product 2 and trifolium height of seedling all greater than control group.
| Process | Corn | Soybean | Trifolium |
| Product 2 | 6.9 | 5.1 | 4.3 |
| Control group | 6.9 | 4.7 | 4.0 |
Claims (9)
1. contain the liquid composite microbiological fertilizer of polyglutamic acid, it is characterized in that, the described composition that contains the liquid composite microbiological fertilizer of polyglutamic acid comprises microbial fermentation bacterium liquid, compound nutrients, gamma-polyglutamic acid-; Described microbial fermentation bacterium liquid: compound nutrients: the mass ratio of gamma-polyglutamic acid-is 1:1:0.05-0.1;
Consisting of of described microbial fermentation bacterium liquid: the fermented liquid of bacillus thuringiensis (Bacillus thuringiensis), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), three kinds of bacterial strains of bacillus megaterium (Bacillus megaterium) mixes with the mass ratio of 1:1:1;
Described compound nutrients comprises nitrogen, phosphorus, potassium element, trace element, organic matter, aminoacids complex, wheat grass meal.
2. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, is characterized in that, described nitrogen element is at least a in urea, ammonium sulfate, ammonium chloride.
3. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, is characterized in that, described phosphoric is at least a in monoammonium phosphate, diammonium phosphate, calcium superphosphate, potassium primary phosphate.
4. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, is characterized in that, described potassium element is at least a in vitriolate of tartar, Repone K, salt of wormwood, saltpetre.
5. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, is characterized in that, described trace element consists of: calcium, magnesium, silicon, zinc, manganese, boron, iron, copper, all add with the form of its soluble compound.
6. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, is characterized in that, described organic matter is humic acids or soluble proteins or both mixtures.
7. the liquid composite microbiological fertilizer that contains polyglutamic acid according to claim 1, it is characterized in that the consisting of of described aminoacids complex: L-Ala, arginine, aspartic acid, Gelucystine, L-glutamic acid, glycine, Histidine, Methionin, methionine(Met), phenylalanine, proline(Pro), Serine, Threonine, tryptophane, tyrosine, α-amino-isovaleric acid.
8. according to claim 1-7 arbitrary described preparation methods that contain the liquid composite microbiological fertilizer of polyglutamic acid, is characterized in that, step is as follows:
(1) actication of culture
Bacillus thuringiensis, bacillus amyloliquefaciens, the activation of bacillus megaterium bacterial strain that 4 ℃ of inclined-planes are preserved, after with its streak inoculation on LB solid plate substratum, cultivate 24h;
(2) seed liquor preparation
The bacterium colony of picking activation is inoculated in the LB liquid nutrient medium, and 37 ℃, 180rpm are cultivated 20h;
(3) fermentation culture
Cultured seed liquor is seeded to fermention medium with 10% inoculum size, 37 ℃, rotating speed 300rpm, ventilating ratio 1:1, collecting zymocyte liquid after fermentation 20h is liquid bacterial fertilizer; Described fermention medium consists of: by percentage to the quality, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium primary phosphate, 0.15% urea, 0.5% yeast powder, 0.41 ‰ sal epsom, all the other are water, pH7.0-7.2;
(4) diatomite is fixed
Add 10% diatomite with fixing thalline in the zymocyte liquid that step (3) is collected and promote the thalline dormancy;
(5) add compound nutrients and polyglutamic acid
The mass ratio of above-mentioned three kinds of bacterium liquid with 1:1:1 mixed, compound nutrients is proportionally added mixing, proportionally successively compound nutrients and gamma-polyglutamic acid-are added in the bacterium liquid that mixes, fully mixing;
(6) can
Above-mentioned mixing liquid is added in the Plastic Bottle of uv sterilisation, paste Product labelling, namely get composite microbiological fertilizer of the present invention.
9. according to claim 1-7 arbitrary described application of liquid composite microbiological fertilizer in soil application that contain polyglutamic acid.
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