CN103168781B - Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent - Google Patents
Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent Download PDFInfo
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- CN103168781B CN103168781B CN201310098229.9A CN201310098229A CN103168781B CN 103168781 B CN103168781 B CN 103168781B CN 201310098229 A CN201310098229 A CN 201310098229A CN 103168781 B CN103168781 B CN 103168781B
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Abstract
The invention discloses a compound sterilizing agent and application thereof. The compound sterilizing agent consists of fluopicolide and fludioxonil with the mass ratio of (1-9): (1-9), the preferred mass ratio of (1-7): (1-5) or the more-preferred mass ratio of (1-5): (1-3). The compound sterilizing agent can be used for preventing and treating plant diseases such as vegetable downy mildew, late blight, gray mold, cucumber root rot and pepper phytophthora blight. The compound sterilizing agent disclosed by the invention is good in quick acting and persistence, has a unique action mechanism, more action sites and a wide bacteriocidal spectrum, is high in biosafety, simple in preparation process and low in cost and has obvious economic benefits.
Description
Technical field
The invention belongs to pesticide field, be specifically related to composite bactericide and the application thereof of a kind of containing fluopicolide and fludioxonil.
Background technology
Vegetables oomycetes disease is caused by Mastigomycotina oomycetes is infected, wherein, downy mildew mould with epidemic disease, the rotten oomycetes such as mould are important pathogen, the vegetable crops such as main harm Cruciferae, Solanaceae, Curcurbitaceae, cause damping off, pythium rot, epidemic disease, cotton disease, downy mildew and root rot etc., cause the significantly underproduction even to have no harvest
[1].Especially downy mildew and phytophthora short incubation period, then infect often, within a season of growth of plant, germ can develop rapidly and cause plant disease epidemic, causes the heavy losses of agricultural production.As cucumber downy mildew can cause cucumber plant all dead in several days
[2].
Botrytis cinerea (Botrytis cinerea) causes the gray mold of most crop, can infect the multiple fruit trees and vegetables such as tomato, eggplant, cucumber, custard squash, grass mould, grape, Kidney bean.Gray mold is a kind of global important disease, is a kind of gas borne disease, and the flower of crop, really, leaf and stem all can fall ill
[3].This disease generally occurs throughout the country, and in rising trend.Ash arrhizus bacteria host range is very extensive, not only can infect tomato and also can infect about 235 kinds of crops such as eggplant, cucumber, capsicum, strawberry, grape, garlic, romaine lettuce, oranges and tangerines
[4-6].
Cucumber root rot is often caused by multiple pathogen Combined Infection, as fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and Phytophthora Root Rot (Phytophthora drechsleri) etc., all use 30% to dislike mould Lingshui Spring agent 800 times of liquid, 3% and dislike mould first frost aqua 650 times of liquid irrigating roots.In addition, fusarium root rot and Phomopsis root rot also available 12.5% carbendazim soluble powder 250 times of liquid, 60% good fortune. first sulphur wetting powder 700 times of liquid, 50% chlorobromide isocyanuric acid soluble powder, 1000 times of liquid irrigating roots.Rotten mould root rot and phytophthora root rot also available 25% metalaxyl wetting powder 600 times of liquid, 50% fluorine mofetil aluminium wetting powder, 600 times of liquid etc. fill with root.Every strain consumption about 200 milliliters when filling with root, fills with if desired one twice again.Above-mentioned effect of chemical control is often very poor, urgently develops new and effective medicament in production, improves its control efficiency to cucumber root rot.
Using chemical agent is the most effective means of controlling plant diseases, but the applicable single bactericide of long-term high dose continuously, easily causes the problems such as the remaining of medicament, environmental pollution and drug resistant fungal development.Composite or the mixture of rational chemical bactericide has expansion fungicidal spectrum, improves control efficiency, extends dispenser interval, reduces dosage, reduces poisoning, reduces and remain, delay disease fungus drug resistance and the feature such as drug-fast generation and development.Bactericide compounded is one of effective ways solved the problem.
Benzamide series bactericidal agent; representative products is fluopicolide; to Plasmopara viticola, bacterium of downy mildew of cucumber, phytophthora infestans, Phytophthora infestans germ and downy mildew of lettuce bacterium etc., there is comparatively high bacteriostatic activity; its mechanism of action is the combination of interference class spectrin and other components; destroy the stability of cytoskeleton; suppress the release of zoospore, sporangial formation and sprouting and mycelial growth, there is protection and therapeutic action
[7,8].68.75% silver medal method profit SC is the complex preparation of fluopicolide and Propamocarb, has remarkable preventive effect to oomycetes diseases such as downy mildew.
Fludioxonil is a kind of pyroles bactericide of novel structure, mainly through suppressing the phosphorylation of MAPK/histidine kinase in disease fungus infiltration intracellular signaling, many alcohols such as the glycerine in thalline are caused to increase, thus suppress conidia germination, germ tube to extend and mycelial growth, finally cause germ dead
[9].Pyroles bactericide is the novel non-internal-absorting wide-spectrum bactericide of a class of the nitre pyroles antibiotic derivative exploitation produced by pseudomonas
[10,11], mainly comprise fludioxonil, fenpiclonil and fluorine acid imide three kinds, this series bactericidal agent, due to characteristics such as efficient, safety, low consumptions, is chosen as by EPA the product that is free from risk
[12].It is reported, fludioxonil is to penicillium expansum (Penicillium expansum), Botrytis cinerea (Botrytis cinerea)
[13], rhizoctonia (Rhizoctonia spp.)
[14], rod method (Alternaria spp.)
[15]good inhibitory action is all had Deng the mycelial growth of pathogen and spore germination.Fludioxonil is in China mainly through seed treatment or soil treatment controlling plant diseases, but fludioxonil is developed as wetting powder by Syngenta Co., Ltd over the past two years, is used for spraying control tomato or ornamental plant gray mold, and shows excellent control efficiency
[16].
So far there is not yet the report utilizing fluopicolide and the control of fludioxonil development mixture cucumber downy mildew, potato/tomato late blight, eliminating vegetable botrytis and the disease such as cucumber root rot and capsicum epidemic disease.
Bibliography:
[1] Si Nai state, Liu Junli. the chemical control present situation of oomycetes disease and control strategy [J]. agricultural chemicals, 2000,39 (2): 7-10.
[2] Dong Jingao chief editor. Agricultural Plant Pathology. the north this [M]. Chinese agriculture publishing house, 2001.5.
[3] Kang Lijuan, Zhang little Feng, Wang Wenqiao, etc. the pesticide resistance of botrytis cinerea and fitness test [J]. Pesticide Science journal, 2003,2:39-42.
[4] wangdan. the comparative studies of Different Crop ash arrhizus bacteria biological property and biological and ecological methods to prevent plant disease, pests, and erosion research [D] of graw mold of tomato. Sichuan: Sichuan Agricultural University, 2008.
[5] Li Baoju. the research of greenhouse tomato gray mold regularty of epidemic. see: the chief editors such as Feng Lanxiang. Tomato in China damage by disease and insect and anti-progress of making a study of subjects thereof. Beijing: Chinese agriculture publishing house, 1998,31-35.
[6] Zhang Congyu, Zhang Juquan, Hu Nengbing, etc. botrytis cinerea host range and Virulence research [J]. Anhui Technology Normal College's journal, 2003,17 (3): 239-242.
[7] Liu Changling. complete works of bactericide volume [M] of world pesticide. Chemical Industry Press, 2006:203-205.
[8]Takeshi?O，Terumasa?K，Shigeru?M.Development?of?a?novel?fungicide，cyazofamid[J].Pesticide?Science，2004，29：136-138.
[9]Hagiwara?D，AsanoY，Marui?J，etal.Transcriptional?profiling?for?Aspergillus?nidulans?HogA?MAPK?signaling?pathway?in?response?to?fludioxonil?and?osmotic?stress[J].Fungal?Genetics?and?Biology，2009，46(11)：868-878.
[10]Gehmann?K，Nyfeler，R，Leadbeater，A?J，etal.A?new?phenylpyrrole?fungicide?for?broad-spectrum?disease?control[A].Brighton?Crop?Prot.Conf.on?Pests?and?Dis[C].UK，1990：369-376.
[１１]Errampalli，D.Effect?of?fludioxonil?on?germination?and?growth?of?Penicillium?expansum?and?decay?in?apple?cVs.Empire?Gala[J].Crop?Protection，2004，23：811-817.
[12] Yan Xiaomin, Ning Binke, Wang Lieping, etc. new type bactericide fluorine fludioxonil and New Progresses of R & D [J] thereof. world pesticide, 2010,12 (3): 32-38.
[13]Leroux，P.Recent?developments?in?the?mode?of?action?of?fungicides[J].Pesticide?Management?Science，1996，47(2)：191-197.
[14]Iacomi-Vasilescu?B，Avenot?H，Bataiué-Simoneau?N，etal.In?vitro?fungicide?sensitivity?of?Alternaria?species?pathogenic?to?crucifers?and?identification?of?Alternaria?brassicicola?field?isolates?highly?resistant?to?both?dicarboximides?and?phenylpyrroles[J].Crop?Protection，2004，23：481-488.
[15]Hamada?M?S，Yin?Y?N，Ma?Z?H.Sensitivity?to?iprodione，difenoconazole?and?fludioxonil?of?Rhizoctonia?cerealis?isolates?collected?from?wheat?in?China[J].Crop?Protection，2011，30：1028-1033.
[16] Ji Junjian, Wang Wenqiao, Zhang little Feng, etc. dispenser and indoor fungicide tame are on the impact [J] of tomato Botrytis cinerea on fludioxonil susceptibility continuously. Pesticide Science journal, 2012,14 (1): 35-41.
Summary of the invention
For the weak point that existing composite bactericide exists, the invention provides a kind of composite bactericide preventing and treating vegetable downy mildew, late blight, gray mold and the disease such as cucumber root rot and capsicum epidemic disease of novel, efficient, safety, applied range.
Composite bactericide provided by the present invention is made up of fluopicolide and fludioxonil, and its mass ratio is 1 ~ 9: 1 ~ 9.
Preferably, the mass ratio of fluopicolide and fludioxonil is 1 ~ 7: 1 ~ 5.
More preferably, the mass ratio of fluopicolide and fludioxonil is 1 ~ 5: 1 ~ 3.
Most preferably, the mass ratio of fluopicolide and fludioxonil is 1: 1.
Present invention also offers a kind of compound sterilizing agent formulation.
Compound sterilizing agent formulation provided by the present invention, is made up of auxiliary agent acceptable on above-mentioned composite bactericide and pesticidal preparations.Wherein, described composite bactericide can account for 20% of the described composite bactericide quality of the pharmaceutical preparations.
Described compound sterilizing agent formulation can be the pesticidal preparations of any one formulation, one or more formulations in preferred following formulation: soluble powder (SP), soluble granula (SG), water dispersible granules (WG), aqua (AS), wetting powder (WP), soluble liquid (SL), missible oil (EC), aqueous emulsion (AE), microemulsion (ME) and suspending agent (SC).
Above-mentioned formulation all can adopt method well-known in the art to prepare, normally by active substance and auxiliary agent, filler, abundant mixed dissolution or grinding or granulation, make preparation to use, the auxiliary agent used in fungicide preparation of the present invention comprise dispersant, wetting agent, binding agent, thickener, anti-foaming agent, disintegrant, filler etc. and other to be of value to active substance stable and play the material of drug effect in the formulation, be not particularly limited, concrete component and consumption are determined by test.
Described compound sterilizing agent formulation, its composition specifically can comprise the component of following mass parts:
Fluopicolide 1 ~ 9 part
Fludioxonil 1 ~ 9 part
Filler is appropriate
Described compound sterilizing agent formulation also can comprise the component of following mass parts further:
Said components is 0 time different.
When described compound sterilizing agent formulation is wetting powder, its composition comprises the component of following mass parts:
Described wetting powder also can comprise the component of following mass parts further: dispersant 0 ~ 8 part, but does not comprise 0 part.
Its preparation process is as follows: according to said ratio, by various composition through mix and blend, enters one-level and pulverizes (grain fineness reaches 80 orders); After carrying out mix and blend again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.
When described compound sterilizing agent formulation is water dispersible granules, its composition comprises the component of following mass parts:
Described water dispersible granules also can comprise the component of following mass parts further: dispersant 0 ~ 8 part, but does not comprise 0 part.
Its preparation process is: according to said ratio by fluopicolide, fludioxonil, dispersant, wetting agent and filler through mix and blend; air-flow crushing obtains wetting powder; then wetting powder and water (or with binding agent) are added in kneader simultaneously mediate; make plastic material; finally send this material to Squeezinggranulator; carry out granulation, obtain water dispersible granules product by dry, screening.
Described dispersant is one or more the mixture in lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), 3-oleoyl amino-2-methoxy benzenesulfonic acid sodium (LS), sanlose (CMC), alkylphenol polyoxyethylene, ethylene glycol monobutyl ether, polyoxyethylene alkyl ether and neopelex.
Described lignin sulfonic acid sodium salt specifically can be: sodium lignin sulfonate (M-14), desugar sodium lignin sulfonate (M-9), lignosulfite.Described alkylphenol polyoxyethylene specifically can be: octyl phenol polyoxyethylene (10) ether (OP-10), polyoxyethylene nonylphenol ether.
Described wetting agent is at least one in sodium alkyl benzene sulfonate, alkyl benzene calcium sulfonate, Negel, fatty alcohol-polyoxyethylene ether (JFC), alkylphenol-polyethenoxy base formaldehyde condensate sulfonate (SOPA), wgwin D30, soapberry powder and bay alcohol radical sodium sulphate.
Described sodium alkyl benzene sulfonate specifically can be neopelex.Described Negel specifically can be nekal (Nekal BX).
Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite and bentonite.
Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic and polyvinyl alcohol.
Described thickener is one or more in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate and polyvinyl alcohol.
Described anti-foaming agent is silicone anti-foaming agent M10 or anti-foaming agent C.
Described disintegrant is one or more in bentonite, calcium chloride, anhydrous sodium sulfate, kaolin, diatomite, ammonium sulfate, urea, sodium phosphate trimer and sodium pyrophosphate.
Present invention also offers the application of above-mentioned bactericidal composition and compound sterilizing agent formulation.
The application of bactericidal composition provided by the present invention and compound sterilizing agent formulation is its application in controlling plant diseases.Described plant disease refers in particular to the disease of melon and fruit and/or vegetables.
Described plant disease comprises oomycetes disease, gray mold and root rot; Described oomycetes disease comprises downy mildew, late blight, capsicum epidemic disease etc.Specifically can be cucumber downy mildew, potato late blight, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease and cucumber root rot etc.
Beneficial effect of the present invention is:
20% fluopicolide fludioxonil WP180g a.i./hm of the present invention
2(1000 times of liquid), 200g a.i./hm
2(900 times of liquid), 250g a.i./hm
2(700 times of liquid) spraying prevents and treats cucumber downy mildew, potato/tomato late blight, reaches 80.1% ~ 92.6% to the effect of cucumber/graw mold of tomato and capsicum epidemic disease; The effect of filling with root control cucumber root rot reaches 49.1% ~ 63.7%.This composite bactericide quick-acting and lasting effect good, mechanism of action is unique, and action site is many, effectively can delay the drug-fast generation of pathogen and development.This complex preparation preparation technology is simple, and cost is low, remarkable in economical benefits, and diseases prevention spectrum is wide, and biological safety is high.
Embodiment
Below by specific embodiment, the present invention will be described, but the present invention is not limited thereto.
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all can obtain from commercial channels.
Medicine in embodiment and experimental example: the former medicine of 96% fludioxonil, 50% fludioxonil WP are all purchased from first just reaching (China) Investment Co., Ltd; The former medicine of 96% fluopicolide, 68.75% fluopicolide Propamocarb SC are all purchased from Bayer Cropscience (China) Co., Ltd; 50% dimethomorph WP, 50% Boscalid WG are all purchased from German BASF AG." % " in following embodiment is mass percentage.
The embodiment of compound sterilizing agent formulation
Embodiment 1: the wetting powder of composite bactericide
Preparation: 20% fluopicolide fludioxonil WP (1: 1, w/w): get the former medicine 10.42kg of 96% fluopicolide, the former medicine 10.42kg of 96% fludioxonil, add 2kgM-9,1.5kgCMC, 5kg neopelex, 70.67kg fine particle calcium carbonate, through mix and blend, enter one-level and pulverize (grain fineness reaches 80 orders); Again after row mix and blend, enter secondary and pulverize (grain fineness reaches 325 orders), be packaged as product.
Embodiment 2-6: wetting powder
Preparation method with embodiment 1, unlike the component that table 1 is listed.
Table 1 embodiment 2-6 component
Embodiment 1-6 composite bactericide fluopicolide fludioxonil WP quality testing
1) active constituent content: the content utilizing liquid chromatography for measuring fluopicolide and fludioxonil, result shows that the content of active ingredient in embodiment 1-6 fluopicolide fludioxonil WP is all greater than 20.00%.
2) wettability determination: carry out according to the GB/T5451-2001 agriculture wetting powder wettability determination method that wraps with straw.The MEBO ribbon gauze of embodiment 1-6 fluopicolide fludioxonil WP is 55s.
3) mensuration of suspensibility: carry out according to GB/T14825-2006 pesticide wettable suspensibility assay method.The suspensibility of embodiment 1-6 fluopicolide fludioxonil WP reaches more than 85%.
4) mensuration of fineness: carry out according to the wet screening in GB/T16150-1995 pesticide wettable fineness assay method.98.5% is reached by the embodiment 1-6 fluopicolide fludioxonil WP of 325 mesh standard sieves (i.e. particle diameter < 44 μm).
5) mensuration of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Moisture≤1.8% in embodiment 1-6 fluopicolide fludioxonil WP.
6) mensuration of mobility: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.Result shows that the flow index of embodiment 1-6 fluopicolide fludioxonil WP is 1.
7) mensuration of foaming characteristic: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The foaming volume of embodiment 1-6 fluopicolide fludioxonil WP is 5mL, meets national standard.
8) mensuration of thermodynamic stability: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.After the storage of embodiment 1-6 fluopicolide fludioxonil WP heat, resolution ratio≤5% of active ingredient, meets national standard.
The water dispersible granules of embodiment 7 composite bactericide
Preparation: 20% fluopicolide fludioxonil WP (1: 1; w/w): get the former medicine 10.42kg of 96% fluopicolide; the former medicine 10.42kg of 96% fludioxonil; add NNO2kg; CMC1.5kg; neopelex 5kg; white carbon 63.67kg; through mix and blend, air-flow crushing obtains can wet-milling, can add in kneader simultaneously mediate by wet-milling with 2kg cyclodextrin and 5kg water; make plastic material; finally send this material to Squeezinggranulator, carry out granulation, obtain water dispersible granules product by dry, screening.
Embodiment 8-12: the water dispersible granules of composite bactericide
Preparation method with embodiment 7, unlike component listed by table 2.
Table 2 embodiment 8-12 component
Embodiment 7-12 composite bactericide fluopicolide fludioxonil WG quality testing
1) active constituent content measuring: the content utilizing liquid chromatography for measuring fluopicolide and fludioxonil.Result shows, in embodiment 7-12, in fluopicolide fludioxonil WG, the content of active ingredient is all greater than 20.00%.
2) determination of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Result shows, moisture≤1.8% in embodiment 7-12 fluopicolide fludioxonil WG.
3) dispersed mensuration: the dispersiveness adopting long tube test method determination embodiment 7-12 fluopicolide fludioxonil WG, result is the sediments bottom pipe is 0.1mL.
4) wettability determination: adopt measuring graduates test method determination.Result shows, the wetability of embodiment 7-12 fluopicolide fludioxonil WG is good.
5) disintegrative measures: to 100mL tool plug graduated cylinder (the interior high 22.5cm containing 90mL distilled water, internal diameter 28cm) at 25 DEG C, add sample particle (0.5g, 250 ~ 1410 μm), clamp the middle part of graduated cylinder afterwards, clog bung, with the speed of 8r/min around central rotation, until sample complete disintegration in water.Result shows, the disintegration time of embodiment 7-12 fluopicolide fludioxonil WG is 2min.
6) suspension stability: measure according to GB/T14825-2006 method.Result is that the suspensibility of embodiment 7-12 fluopicolide fludioxonil WG reaches more than 90%.
7) mensuration of thermodynamic stability: enclosed by sample in ground wide-mouth bottle, after storing 4 weeks, analyzes embodiment 7-12 fluopicolide fludioxonil WG active constituent content in (50 ± 1) DEG C insulating box.Result shows that active ingredient resolution ratio is less than 5%.
Embodiment 13: the aqueous emulsion of composite bactericide
Preparation: 20% fluopicolide fludioxonil WP (1: 1, w/w): get the former medicine 10.42kg of 96% fluopicolide, the former medicine 10.42kg of 96% fludioxonil, adds xanthans 1.2kg, NNO0.8kg, CMC1.5kg, neopelex 5kg, through mix and blend, under high velocity agitation, water 70.66kg is joined in oil phase, forms the aqueous emulsion of favorable dispersibility.Quality testing meets relevant criterion.
Embodiment 14: the suspending agent of composite bactericide
20% fluopicolide fludioxonil WP (1: 1, w/w): get the former medicine 10.42kg of 96% fluopicolide, the former medicine 10.42kg of 96% fludioxonil, adds NNO2kg, CMC1.5kg, neopelex 5kg, water 70.66kg take water as medium, active component, auxiliary agent is added in sand milling still to mix with abrasive media and grinds, obtain homogeneous suspension liquid body, liquid is separated obtained suspending agent with abrasive media.Quality testing meets relevant criterion.
The bactericidal effect of compound sterilizing agent formulation
Experimental example 1: different ratio composite bactericide is to the virulence test of cucumber root rot bacterium
1.1 for examination material
1.1.1 for examination bacterial classification: cucumber root rot bacterium, picking up from Zhao Cun township, Dingzhou City in Hebei Province Xi Gandecun, having fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and Phytophthora Root Rot (Phytophthora drechsleri) through being separated pathogen identification.
1.1.2 for examination medium: potato dextrose agar (PDA) medium (potato 200g, glucose 20g, agar 20g, deionized water 1000mL).
1.1.3 supply reagent product: the wetting powder of embodiment 1-6, fluopicolide and fludioxonil quality proportioning are 1 ~ 4: 1 ~ 3, prepare the test medicine of fluopicolide and fludioxonil quality proportioning 9 ~ 1: 1 ~ 9 equally, carry out virulence test with former medicine.
1.2 test method
1.2.1 colony diameter mensuration bactericide and mixture are to the virulence of cucumber root rot bacterium
By the former medicine of 96% fluopicolide and the former medicinal acetone solution of 96% fludioxonil, be diluted to the liquid of series concentration again, in liquid: the ratio of medium (PDA) volume ratio 1: 9, add in the medium being cooled to about 60 DEG C after dissolving and mix, add the culture dish of diameter 9cm, make pastille PDA dull and stereotyped, blank adds the coordinative solvent of equivalent; Be that the card punch of 5mm is beaten along colony edge and got bacterium cake by the cucumber root rot bacterial strain diameter of preculture 4d, be inoculated into the PDA culture medium flat plate central authorities containing series concentration, every ware 1 ferfas cake, mycelia faces down, cover ware lid, often process repetition 3 times, be placed in after 4d cultivated by 25 DEG C of incubators and adopt right-angled intersection method to measure colony diameter.Calculate the inhibiting rate of each concentration liquid, utilize DPS Software of Data Statistics to obtain virulence regression equation (Y=a+bx), the EC of fluopicolide, fludioxonil and composition thereof suppression cucumber root rot mycelial growth
50value and correlation coefficient.
Inhibiting rate (%)=(contrast bacterium colony increases diameter-process bacterium colony and increases diameter) × 100/ (contrast bacterium colony increases diameter)
1.2.2 the virulence synergist of fungicide compounding
According to Wadley (1967) the formulae discovery not mixed coefficient of synergism of with medicament:
SR＝EC
50(th)/EC
50(ob)
In above formula, a, b represent fluopicolide (A), fludioxonil (B) two kinds of bactericide shared ratio in the mixture; EC
50and EC (A)
50(B) the actual observation EC of A and B is represented respectively
50value, EC
50(th) represent that a: b mixed theoretical EC pressed by A, B two kinds of bactericide
50value, EC
50(ob) for a: b mixed actual observation EC pressed by A, B two kinds of bactericide
50value.SR > 1.5, synergy; SR < 0.5, antagonism; SR represents addition between 0.5 and 1.5.
2. result of the test
As known from Table 3, fluopicolide and fludioxonil mixture suppress the synergy proportioning of cucumber root rot bacterium mycelial growth coefficient of synergism in 5 ~ 1: 1 ~ 3 scopes to be greater than 1.5, have good synergistic effect, when wherein fluopicolide and fludioxonil mass ratio are 1: 1, synergy is the strongest.
Table 3 fluopicolide and fludioxonil mixture are to the virulence synergist of cucumber root rot bacterium
Experimental example 2: embodiment 1 composite bactericide wetting powder (WP) prevents and treats the effect of cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato, cucumber root rot and capsicum epidemic disease
1. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the cucumber downy mildew test of pesticide effectiveness
Test according to " bactericidal agent for preventing and treating cucumber downy mildew test of pesticide effectiveness criterion " in medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Susceptible variety is cucumber variety " capital is green ".Experimental field is positioned at Dingzhou City Zhao Cun township of Hebei province Xi Gandecun, breed selection, field planting on January 25 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area is about 18m
2.
Start to spray medicine before downy mildew naturally-occurring, share medicine 3 times, interval is 7 days.Spraying time is respectively on March 5th, 2012, March 12, March 19.Jacto-HD400 type manual sprayer is adopted evenly to spray medicine.Be 900L/hm by amount of liquid medicine
2.
Investigation morbidity in 7 days (on March 26th, 2012) after last 1 dispenser.Random 4 sampling surveys in every community, often some investigation 2 strains, whole blade is investigated in every strain, and the percentage accounting for whole blade area by lesion area on each blade divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole leaf area; 3 grades: scab accounts for 6% ~ 10% of whole leaf area; 5 grades: scab accounts for 11% ~ 25% of whole leaf area; 7 grades: scab accounts for 26% ~ 50% of whole leaf area; 9 grades: scab accounts for more than 51% of whole leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 4,20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good control efficiency (80.8% ~ 91.2%) to cucumber downy mildew.Spray 20% fluopicolide fludioxonil WP250g a.i./hm
2preventive effect be 91.2%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm
2control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm
2control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm
2preventive effect be 86.2%, and spray 50% dimethomorph WP375g a.i./hm
2preventive effect suitable.Show to adopt 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm before cucumber downy mildew occurs or when just occurring
2foliage-spray has good control efficiency to cucumber downy mildew.
Table 4 20% fluopicolide fludioxonil WP is to the control efficiency of cucumber downy mildew
| Process | Sick leaf rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 8.35 | 2.49 | 80.8c |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 6.17 | 1.80 | 86.2b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 5.10 | 1.15 | 91.2a |
| 68.75% fluopicolide propamocarb SC1031g a.i./hm 2 | 4.07 | 0.98 | 92.5a |
| 50% dimethomorph WP375g a.i./hm 2 | 7.30 | 1.63 | 87.5b |
| Blank | 23.09 | 13.10 | - |
Note: with indifference between lowercase alphabet registration certificate identical after columns pick significantly (P=0.05).Lower same.
2. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats potato late blight test
Test according to " bactericidal agent for preventing and treating potato late blight test of pesticide effectiveness criterion " in medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Local susceptible variety is Potato Cultivars " Favorita ".Experimental field is positioned at Shizigou township, Chongli County, Hebei province seed farm, and on April 25th, 2012 plants, and line-spacing 0.6m, spacing in the rows 0.3m, potato late blight occurs over the years.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement.Plot area 20m
2if, protection row.
Start to spray medicine before field natural occurrence, share medicine 3 times, 7 ~ 10 days intervals.Spraying time is respectively June 20, June 27, July 4.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, spraying is evenly thoughtful.Be 900L/hm by amount of liquid medicine
2.Whole duration of test affects without harsh weather.
Investigation in 7 days (on July 11st, 2012) incidence after last dispenser.Every community diagonal 5 sampling surveys, often some investigation 2 strains, whole compound leaf is investigated in every strain, and the percentage accounting for whole compound leaf area by lesion area on each compound leaf divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole compound leaf area; 3 grades: scab accounts for 6% ~ 10% of whole compound leaf area; 5 grades: scab accounts for 11% ~ 25% of whole compound leaf area; 7 grades: scab accounts for 26% ~ 50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index and preventive effect.In data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100 as known from Table 5,20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good control efficiency (83.4% ~ 90.9%) to potato late blight.Spray 20% fluopicolide fludioxonil WP250g a.i./hm
2preventive effect be 90.9%, be significantly higher than Suo and execute 50% dimethomorph WP375g a.i./hm
2control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm
2control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm
2preventive effect be 87.5%, and spray 50% dimethomorph WP375g a.i./hm
2preventive effect suitable.Show before potato late blight occurs or when just occurring, adopt 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good preventive effect to potato late blight.
Table 5 20% fluopicolide fludioxonil WP is to the control efficiency of potato late blight
| Process | Sick leaf rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 9.82 | 2.06 | 83.4c |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 6.84 | 1.55 | 87.5b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 5.69 | 1.13 | 90.9a |
| 68.75% fluopicolide propamocarb SC1031g a.i./hm 2 | 4.31 | 1.02 | 91.7a |
| 50% dimethomorph WP375g a.i./hm 2 | 6.36 | 1.43 | 88.4b |
| Blank | 22.13 | 12.38 | - |
3. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the tomato late blight test of pesticide effectiveness
Experimental field is positioned at Xin Xing village of Dingzhou City Yang Jiazhuan township of Hebei province, is breed selection tomato, and tomato variety is susceptible variety " Borrow's tower ".On February 18th, 2012 plants, line-spacing 0.8m, spacing in the rows 0.3m.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on April 18th, 2012, April 25, May 2.Jacto-HD400 type manual sprayer is adopted evenly to spray medicine.Be 900L/hm by amount of liquid medicine
2.
Investigation in 7 days (on May 9th, 2012) incidence after last 1 dispenser.Random 5 sampling surveys in every community, often some investigation 2 strains, whole compound leaf is investigated in every strain, and the percentage accounting for whole compound leaf area by lesion area on each compound leaf divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole compound leaf area; 3 grades: scab accounts for 6% ~ 10% of whole compound leaf area; 5 grades: scab accounts for 11% ~ 25% of whole compound leaf area; 7 grades: scab accounts for 26% ~ 50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 6,20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good control efficiency (82.0% ~ 91.3%) to tomato late blight.Spray 20% fluopicolide fludioxonil WP250 g a.i./hm
2preventive effect be 91.3%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm
2control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm
2control efficiency suitable.Spray 20% fluopicolide fludioxonil WP200g a.i./hm
2preventive effect be 87.6%, and spray 50% dimethomorph WP375g a.i./hm
2preventive effect suitable.Show before tomato late blight occurs or when just occurring, adopt 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good preventive effect to tomato late blight.
Table 6 20% fluopicolide fludioxonil WP is to the control efficiency of tomato late blight
| Process | Sick leaf rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 8.69 | 1.72 | 82.0c |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 5.86 | 1.21 | 87.6b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 4.52 | 0.82 | 91.3a |
| 68.75% fluopicolide propamocarb SC1031g a.i./hm 2 | 3.72 | 0.69 | 92.7a |
| 50% dimethomorph WP375g a.i./hm 2 | 5.77 | 1.18 | 87.3b |
| Blank | 17.73 | 9.54 | - |
4. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the capsicum epidemic disease test of pesticide effectiveness
In medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ", " bactericidal agent for preventing and treating capsicum epidemic disease test of pesticide effectiveness criterion " carries out.Susceptible variety is capsicum variety " the ears of an ox or cow king ".Experimental field is positioned at the solid cities and towns Bei Zhuantoucun in Dingxing County of Hebei province, is breed selection capsicum, line-spacing 0.8m, spacing in the rows 0.3m.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 1031g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 24m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on May 4th, 2012, May 11, May 18.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on May 25th, 2012) is investigated.Random 5 sampling surveys in every community, often some investigation 10 strains.Stage division: 0 grade: anosis; 1 grade: rhizome portion is without scab, and indivedual branch or fruit are fallen ill; 3 grades: rhizome portion slightly fall ill the following branch of half or fruit morbidity; 5 grades: rhizome portion moderate morbidity branch more than half or fruit morbidity; 7 grades: seriously fall ill in rhizome portion, the large portion of plant is wilting; 9 grades: the whole wilting death of plant.Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
Diseased plant rate (%)=(diseased plant number/investigate total strain number) × 100
Disease index=∑ (diseased plant number × relative disease value of series at different levels)/(investigating total strain number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 7,20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good control efficiency (86.1% ~ 91.0%) to capsicum epidemic disease.Spray 20% fluopicolide fludioxonil WP250g a.i./hm
2preventive effect be 91.0%, be significantly higher than and spray 50% dimethomorph WP375g a.i./hm
2control efficiency, with 68.75% fluopicolide propamocarb SC1031g a.i./hm
2control efficiency suitable.Spray 20% fluopicolide fludioxonil WP180 ~ 200g a.i./hm
2preventive effect be 86.1% ~ 87.8%, and spray 50% dimethomorph WP375g a.i./hm
2preventive effect suitable.Show to adopt 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm before capsicum epidemic disease occurs or when just occurring
2foliage-spray has good control efficiency to capsicum epidemic disease.
Table 7 20% fluopicolide fludioxonil WP is to the control efficiency of capsicum epidemic disease
| Process | Diseased plant rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 13.50 | 2.06 | 86.1b |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 11.00 | 1.78 | 87.8b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 7.00 | 1.33 | 91.0a |
| 68.75% fluopicolide propamocarb SC1031g a.i./hm 2 | 7.50 | 1.39 | 90.5a |
| 50% dimethomorph WP375g a.i./hm 2 | 9.50 | 2.06 | 86.1b |
| Blank | 25.00 | 15.00 | - |
5. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the gray mold of cucumber test of pesticide effectiveness
Test according to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " GB/T17980.28-2000 " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ".Cucumber variety is conventional variety " De Ruite 787 ".Experimental field is positioned at Dingzhou City Zhao Cun township of Hebei province Xi Gandecun, field planting on February 14 in 2012.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 50% Boscalid WG, dispenser dosage is 300g a.i./hm
2; 50% fludioxonil WP, dispenser dosage is 100g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on March 6th, 2012, March 13, March 20.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on March 27th, 2012) is investigated.Investigation incidence, every community gets 5 points at random, whole fruits of often some investigation 2 strains, the sick fruit number that record gray mold causes.Gray mold of cucumber is investigated in units of fruit: 0 grade: without scab; 1 grade: residual flower morbidity; 3 grades: fall ill in areola portion; 5 grades: scab length accounts for less than 10% of fruit; 7 grades: scab length accounts for 11% ~ 25% of fruit; 9 grades: scab length accounts for more than 26% of fruit.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
Diseased fruit rate (%)=(sick fruit number/investigation total fruit number) × 100
Disease index=∑ (sick fruit number × relative disease value of series at different levels)/(investigation total fruit number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 8,20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliage-spray has good control efficiency (80.1% ~ 89.1%) to gray mold of cucumber.Spray 20% fluopicolide fludioxonil WP250g a.i./hm
2preventive effect be 89.1%, and spray 50% Boscalid WG300g a.i./hm
2control efficiency suitable, be significantly higher than and spray 50% fludioxonil WP100g a.i./hm
2control efficiency.Spray 20% fluopicolide fludioxonil WP200g a.i./hm
2preventive effect be 86.6%, and spray 50% fludioxonil WP100g a.i./hm
2control efficiency suitable.When showing that front or first generation occurs gray mold of cucumber, adopt 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2carry out foliage-spray, to gray mold of cucumber, there is good preventive effect.
Table 8 20% fluopicolide fludioxonil WP is to the control efficiency of gray mold of cucumber
| Process | Epilepsy fruit rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 36.71 | 7.04 | 80.1c |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 28.81 | 4.79 | 86.6b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 22.37 | 3.89 | 89.1a |
| 50% Boscalid WG300g a.i./hm 2 | 19.87 | 3.70 | 89.5a |
| 50% fludioxonil WP100g a.i./hm 2 | 26.79 | 4.79 | 86.4b |
| Blank | 68.75 | 35.55 | - |
6. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the graw mold of tomato test of pesticide effectiveness
According to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " GB/T17980.28-2000 " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ' ' test.Tomato variety is conventional variety " east victory hooligan ".Experimental field is positioned at Tian Cunpu village, Gao Lin villages and small towns, Xushui County, Hebei province, field planting on February 10 in 2012.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Contrast medicament: 50% Boscalid WG, dispenser dosage is 300g a.i./hm
2; 50% fludioxonil WP, dispenser dosage is 100g a.i./hm
2; Blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on March 4th, 2012, March 11, March 18.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on March 25th, 2012) is investigated.Investigation incidence, every community gets 5 points at random, whole fruits of often some investigation 2 strains, the sick fruit number that record gray mold causes.Gray mold fruit grade scale is: 0 grade: without scab; 1 grade: residual petal morbidity or column cap morbidity; 3 grades: sepal rots or column cap morbidity spreads to areola portion; 5 grades: areola portion has and infiltrates spot but without mould layer; 7 grades: there is mould layer in areola portion but does not expand to other position; 9 grades: the mould layer in areola portion expands to other position.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
Diseased fruit rate (%)=(sick fruit number/investigation total fruit number) × 100
Disease index=∑ (sick fruit number × relative disease value of series at different levels)/(investigation total fruit number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 9,20% fluopicolide fludioxonil WP150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (81.4% ~ 91.8%) to graw mold of tomato.Spray 20% fluopicolide fludioxonil WP300g a.i./hm
2preventive effect be 91.8%, and spray 50% Boscalid WG300g a.i./hm
2control efficiency suitable, be significantly higher than and spray 50% fludioxonil WP100g a.i./hm
2control efficiency.Spray 20% fluopicolide fludioxonil WP200g a.i./hm
2preventive effect be 88.0%, and spray 50% fludioxonil WP100g a.i./hm
2control efficiency suitable.20% fluopicolide fludioxonil WP150 ~ 300g a.i./hm is adopted when showing that front or just generation occur graw mold of tomato
2carry out foliage-spray, to graw mold of tomato, there is good control efficiency.
Table 9 20% fluopicolide fludioxonil WP is to the control efficiency of graw mold of tomato
| Process | Diseased fruit rate (%) | Disease index | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP180g a.i./hm 2 | 24.97 | 4.83 | 81.4c |
| 20% fluopicolide fludioxonil WP200g a.i./hm 2 | 18.92 | 3.15 | 88.0b |
| 20% fluopicolide fludioxonil WP250g a.i./hm 2 | 14.16 | 2.09 | 91.8a |
| 50% Boscalid WG300g a.i./hm 2 | 12.39 | 2.07 | 91.9a |
| 50% fludioxonil WP100g a.i./hm 2 | 17.54 | 3.00 | 88.2b |
| Blank | 50.98 | 26.44 | - |
7. 20% fluopicolide fludioxonil WP in embodiment 1 prevents and treats the cucumber root rot test of pesticide effectiveness
Experimental field is positioned at Dingzhou City Zhao Cun township of Hebei province Xi Gandecun, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Test medicine: 20% fluopicolide fludioxonil WP dispenser dosage is 1000 times of liquid, 900 times of liquid, 700 times of liquid.Contrast medicament: 68.75% fluopicolide propamocarb SC, dispenser dosage is 600 times of liquid; 50% fludioxonil WP, dispenser dosage is 4500 times of liquid, and blank fills with clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 24m
2.
Before cucumber root rot occurs, every strain perfusion liquid 250mL.A situation arises to investigate each process root rot every 15d after medicine, and record diseased plant number, calculates control efficiency according to diseased plant rate.In data acquisition SPSS13.0 software, Fisher's least significant difference (LSD) measures and carries out statistical analysis.
As known from Table 10,20% fluopicolide fludioxonil WP1000 ~ 700 times liquid irrigating root has good control efficiency (49.1% ~ 63.7%) to cucumber root rot, is significantly higher than the control efficiency of 68.75% fluopicolide propamocarb SC600 times of liquid and 50% fludioxonil WP4500 times liquid.Show to adopt 20% fluopicolide fludioxonil WP700 ~ 1000 times liquid irrigating root to have good control efficiency to cucumber root rot before cucumber root rot occurs.
Table 10 20% fluopicolide fludioxonil WP is to the control efficiency of cucumber root rot
| Process | Epilepsy strain rate (%) | Control efficiency (%) |
| 20% fluopicolide fludioxonil WP1000 times liquid | 15.50 | 49.1c |
| 20% fluopicolide fludioxonil WP900 times liquid | 12.50 | 59.1b |
| 20% fluopicolide fludioxonil WP700 times liquid | 11.00 | 63.7a |
| 68.75% fluopicolide propamocarb SC600 times liquid | 19.50 | 36.1d |
| 50% fludioxonil WP4500 times liquid | 20.50 | 32.8d |
| Blank | 30.50 | - |
Field trial shows: 20% fluopicolide fludioxonil WP180 ~ 250g a.i./hm
2foliar spray all has good preventive and therapeutic effect to cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato and capsicum epidemic disease, 20% fluopicolide fludioxonil WP700 ~ 1000 times liquid irrigating root has good control efficiency to cucumber root rot, can application in field produces.
Experimental example 3: the composite bactericide fluopicolide fludioxonil control cucumber downy mildew in embodiment 7,13,14
Experimental field is positioned at Dingzhou City Zhao Cun township of Hebei province Xi Gandecun, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Dispenser dosage is 180g a.i./hm
2, 200g a.i./hm
2, 250g a.i./hm
2.Result shows, the fluopicolide fludioxonil WP180 ~ 250g a.i./hm in embodiment 7,13,14
2foliage-spray has good control efficiency (81.4% ~ 92.6%) to cucumber downy mildew.
Fluopicolide fludioxonil in table 15 embodiment 7,13,14 is to the control efficiency of cucumber downy mildew
Claims (7)
1. a composite bactericide, is made up of fluopicolide and fludioxonil; Wherein, the mass ratio of described fluopicolide and fludioxonil is 1 ~ 5:1 ~ 3.
2. a compound sterilizing agent formulation, is made up of auxiliary agent acceptable on composite bactericide according to claim 1 and pesticidal preparations.
3. compound sterilizing agent formulation according to claim 2, is characterized in that: the formulation of described compound sterilizing agent formulation is selected from following at least one: wetting powder, water dispersible granules, soluble powder, soluble granula, aqua, soluble liquid, missible oil, aqueous emulsion, microemulsion and suspending agent.
4. compound sterilizing agent formulation according to claim 2, is characterized in that: described auxiliary agent comprises following at least one: dispersant, wetting agent, binding agent, thickener, anti-foaming agent, disintegrant and filler.
5. composite bactericide according to claim 1 or the application of compound sterilizing agent formulation in controlling plant diseases according to any one of claim 2-4.
6. application according to claim 5, is characterized in that: described plant is melon and fruit and/or vegetables; Described disease comprises: oomycetes disease and gray mold.
7. application according to claim 6, is characterized in that: described plant disease is selected from following at least one: cucumber downy mildew, potato late blight, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease and cucumber root rot.
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