CN103163099B - Antioxidant capacity measuring method based on triangle silver nanoparticle prism - Google Patents
Antioxidant capacity measuring method based on triangle silver nanoparticle prism Download PDFInfo
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- CN103163099B CN103163099B CN201310056995.9A CN201310056995A CN103163099B CN 103163099 B CN103163099 B CN 103163099B CN 201310056995 A CN201310056995 A CN 201310056995A CN 103163099 B CN103163099 B CN 103163099B
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- antioxidant
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- silver nanoparticle
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Abstract
The invention discloses an antioxidant capacity measuring method based on a triangle silver nanoparticle prism. The method comprises the steps of preparation of the triangle silver nanoparticle prism, respective action of adding H2O2 with a certain concentration and mixed liquid of the H2O2 with the concentration and antioxidant with a different concentration into triangle silver nanoparticle prism sol, and calculation of the antioxidant capacity of the antioxidant through variation of nanometer silver colloid spectral quality in the mixed liquid before and after the antioxidant is added. Compared with a traditional way in which absorbance is used as a detecting index, transferring of LSPR absorption peak wavelength of the silver nanoparticle prism is adopted as the detecting index in the method. The method is simple in operation, convenient to operate, low in cost and high in flexibility.
Description
Technical field
The present invention relates to a kind of determination oxidative method based on triangular nano silver prism.
Background technology
Active oxygen comprises hydrogen peroxide (H
2o
2), superoxide radical (O
2 -), hydroxy radical (OH) and downstream product superoxide and hydroxylate etc., active oxygen participates in growth and proliferation of cell, Development And Differentiation, aging and apoptosis and much physiology and pathologic process.Body is when suffering various destructive stimulus, and activity in vivo oxygen produces too much, and degree of oxidation exceeds the removing ability of body, oxidative system and antioxidant system are unbalance, to cause canceration, angiocardiopathy, inflammation, Alzheimer's disease, Parkinson's, diabetes and old and feeble key factor.Increasing evidence shows that the polyphenoils in diet can protect important biomolecule to avoid damage, reduces the generation of the chronic disease relevant with aging.Therefore, that method detects the oxidation resistance of polyphenoils in diet simply, reliably, is fast particularly important in development.
The determination oxidative of antioxidant comprises intracorporal method and in vitro method, and in vitro method is because application with low cost is more extensive.External test method comprises spectrophotometric method, fluorescence method, chemoluminescence method, chromatography, electron spin resonance etc.Wherein spectrophotometric method is to adopt that in visible region, to have the free radical of absorption or neutral molecule be probe, the oxidation resistance of measuring antioxidant according to the variation of absorption spectrum intensity after probe molecule and all kinds of oxygenant or antioxidant action is to apply one of the most general means of testing.Spectrophotometric method comprises ABTS method, DMPD
+method, DPPH method, beta carotene bleaching, crocin bleaching, FRAP method etc.These methods are all usingd the variation of absorption spectrum intensity as measured signal, and common shortcoming is can produce larger deviation when measuring coloured sample.
Summary of the invention
The object of the present invention is to provide a kind of migration of the LSPR absorption peak wavelength that adopts silver nanoparticle prism as the determination oxidative method based on triangle silver nanoparticle prism of measured signal.
Technical solution of the present invention is:
A determination oxidative method based on triangle silver nanoparticle prism, is characterized in that: comprise the following steps: successively
(1) preparation of triangle silver nanoparticle prism: silver nitrate is done with sodium citrate to weak reductant, sodium borohydride are made strong reductant, polyvinylpyrrolidone is made protective agent, prepares triangle silver nanoparticle prism colloidal sol;
(2) in above-mentioned triangle silver nanoparticle prism colloidal sol, add respectively certain density H
2o
2and this concentration H
2o
2each autoreaction of antioxidant mixed liquor with variable concentrations; Utilization adds the change calculations of nanometer elargol spectral quality in the mixed liquor of antioxidant front and back to obtain the oxidation resistance of antioxidant.
The concrete grammar of step (2) is: a) in triangle silver nanoparticle prism colloidal sol, add certain density H
2o
2react 2 ~ 6 minutes, utilize ultraviolet-visible pectrophotometer to measure absorption spectrum, record maximum absorption wavelength;
B) in triangle silver nanoparticle prism colloidal sol, add and the step H that a) concentration is identical
2o
2react with the mixed liquor of certain density antioxidant, the reaction time is identical with the reaction time of step a), measures absorption spectrum, records maximum absorption wavelength, and by formula Ip=(Δ W
0-Δ W)/Δ W
0, calculate the antioxidant of this concentration to H
2o
2clearance rate; Δ W wherein
0refer to triangle silver nanoparticle prism and adding H
2o
2the variation of front and back absorbing wavelength, Δ W is when antioxidant exists, and in silver nanoparticle prism, adds H
2o
2the variation of front and back absorbing wavelength, Ip is clearance rate;
C) antioxidant that calculates gained according to step b) is to H
2o
2clearance rate numerical values recited, by increasing and/or reduce the concentration of antioxidant, calculate under variable concentrations antioxidant to H
2o
2clearance rate; By ORIGIN, draw H
2o
2the relation curve of clearance rate and corresponding antioxidant concentration, and the concentration of reading clearance rate antioxidant while being 50% from figure, this concentration is the IC of selected antioxidant
50value, its unit is g/L; By the IC of ascorbic acid
50value is divided by the IC of each antioxidant
50be worth, can obtain the Vc equivalent of antioxidant, represent oxidation resistance size.
Described antioxidant is citric acid, caffeic acid, gallic acid, ascorbic acid or forulic acid etc.
Triangle silver nanoparticle prism can produce local plasmon resonance (LSPR), thereby causes strong light absorption and scattering.Due in face and face is outer exists when waiting from resonance mode, make silver nanoparticle prism in UV-vis absorption spectrum, conventionally show two LSPR and absorb, and absorbing wavelength and their size and dimension closely related.The present invention uses triangle silver nanoparticle prism to measure the related ultimate principle of oxidation resistance: silver nanoparticle prism can be by H
2o
2the change that is oxidized and produces size and dimension, thus cause the optical property of silver nanoparticle prism to change, and LSPR absorbing wavelength is moved, and antioxidant for clearing H
2o
2after can suppress the generation of this process.Its innovation is to adopt the migration of LSPR absorption peak wavelength of silver nanoparticle prism as measured signal, compares as detecting index with adopting absorbance in classic method, easy and simple to handle, with low cost and highly sensitive.
Compared with prior art, the present invention also tool have the following advantages:
(1) triangle silver nanoparticle prism preparation method is ripe, prepares easyly, and the reagent cost using is cheap.
(2) the absorption peak position 608nm of the triangle silver nanoparticle prism using in the present invention, adds 30 μ M H
2o
2react 3 minutes, absorb peak position and become 548nm, absorption peak wavelength has moved about 60nm, i.e. the H of every variation 1 μ M
2o
2concentration, the about 2nm of wavelength variations, detects sensitive.
(3) because human eye is the most responsive to the variation in above-mentioned peak wavelength coverage, therefore the method also can be used in visual detection qualitatively.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 is determination oxidative principle schematic.
Fig. 2. be oxidation resistance schematic diagram calculation.
Fig. 3, Fig. 4, Fig. 5 are respectively that several antioxidants are to H
2o
2clearance rate and concentration relationship curve map.
Fig. 6 adds the H of 30 μ M in triangle silver nanoparticle colloidal sol
2o
2rear spectrum is schematic diagram over time.
Fig. 7 is citric acid concentration while being 1g/L, adds the H of 30 μ M in triangle silver nanoparticle colloidal sol
2o
2rear spectrum temporal evolution schematic diagram.
Fig. 8 is H
2o
2concentration 30 μ M, add the abosrption spectrogram of the 3 minutes triangle silver nanoparticle colloidal sol of citric acid reactions of variable concentrations.
In Fig. 2, AgNP refers to silver nanoparticle prism (Ag nano-prism).
Embodiment
A determination oxidative method based on triangle silver nanoparticle prism, comprises the following steps: successively
(1) at room temperature put into 100mL, 0.2mM AgNO are housed
3the 250mL conical flask of aqueous solution.While stirring successively, in bottle, add the polyvinylpyrrolidone aqueous solution 6mL of sodium citrate aqueous solution 6mL, the 2mM of 30mM, 30% H
2o
20.24mL, now solution achromaticity and clarification.Then fast to the NaBH that injects 0.1mol/L in bottle
4aqueous solution 0.4mL, solution occurs light yellow at once, after being stirred and being uniformly dispersed, light yellow remaining unchanged.Approximately after 10～15min, solution starts to occur obvious change color, in tens seconds from pale yellow become continuously orange-orange red-purplish red-light blue-dark blue, a large amount of bubbles of simultaneously emerging in solution, obtain triangle silver nanoparticle prism colloidal sol, proceed in cold compartment of refrigerator and can use after standing 12h.
(2) in above-mentioned triangle silver nanoparticle prism colloidal sol, adding respectively reaction system final concentration is the H of 30 μ M
2o
2and this concentration H
2o
2with the citric acid of variable concentrations, under room temperature, react 4 minutes, record ultraviolet-visible absorption spectroscopy, according to maximum absorption wavelength separately, calculate variable concentrations citric acid to H
2o
2clearance rate Ip=(Δ W
0-Δ W)/Δ W
0, calculate the antioxidant citric acid of this concentration to H
2o
2clearance rate; Δ W wherein
0refer to triangle silver nanoparticle prism and adding H
2o
2the variation of front and back absorbing wavelength, Δ W is when antioxidant citric acid exists, and in triangle silver nanoparticle prism, adds H
2o
2the variation of front and back absorbing wavelength, Ip is clearance rate; By ORIGIN, draw H
2o
2the relation curve of clearance rate and corresponding citric acid concentration, and the concentration of reading clearance rate citric acid while being 50% from figure, this is citric acid IC
50=1.5g/L.Same method obtains the IC of ascorbic acid
50value, and by the IC of ascorbic acid
50value is divided by the IC of antioxidant citric acid
50be worth, can obtain the Vc equivalent of antioxidant citric acid.
The oxidation resistance of the several frequently seen antioxidant of table 1
Adopt same method can also obtain the Vc equivalent of the antioxidants such as caffeic acid, gallic acid, forulic acid.
Claims (2)
1. the determination oxidative method based on triangle silver nanoparticle prism, is characterized in that: comprise the following steps: successively
(1) preparation of triangle silver nanoparticle prism: silver nitrate is done with sodium citrate to weak reductant, sodium borohydride are made strong reductant, polyvinylpyrrolidone is made protective agent, prepares triangle silver nanoparticle prism colloidal sol;
(2) a) in triangle silver nanoparticle prism colloidal sol, add H
2o
2react 2～6 minutes, utilize ultraviolet-visible pectrophotometer to measure absorption spectrum, record maximum absorption wavelength;
B) in triangle silver nanoparticle prism colloidal sol, add and the step H that a) concentration is identical
2o
2react with the mixed liquor of antioxidant, the reaction time is identical with step reaction time a), measures absorption spectrum, records maximum absorption wavelength, and by formula Ip=(Δ W
0-Δ W)/Δ W
0, calculate the antioxidant of this concentration to H
2o
2clearance rate; Δ W wherein
0refer to triangle silver nanoparticle prism and adding H
2o
2the variation of front and back absorbing wavelength, Δ W is when antioxidant exists, and in silver nanoparticle prism, adds H
2o
2the variation of front and back absorbing wavelength, Ip is clearance rate;
C) according to step b) antioxidant that calculates gained is to H
2o
2clearance rate numerical values recited, by increasing/or reduce the concentration of antioxidant, calculate under variable concentrations antioxidant to H
2o
2clearance rate; By ORIGIN, draw H
2o
2the relation curve of clearance rate and corresponding antioxidant concentration, and the concentration of reading clearance rate antioxidant while being 50% from figure, this concentration is the IC of selected antioxidant
50value, its unit is g/L; By the IC of ascorbic acid
50value is divided by the IC of each antioxidant
50be worth, can obtain the Vc equivalent of antioxidant, represent oxidation resistance size.
2. the determination oxidative method based on triangle silver nanoparticle prism according to claim 1, is characterized in that: described antioxidant is citric acid, caffeic acid, gallic acid, ascorbic acid or forulic acid.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103817346A (en) * | 2014-03-11 | 2014-05-28 | 上海交通大学 | Shape-controlled triangle flaky nano silver powder preparation method |
| CN106270543B (en) * | 2015-06-12 | 2019-05-07 | 中国科学院大连化学物理研究所 | The method for continuously preparing the controllable Triangular nanoplates of arrangement mode |
| CN106290196A (en) * | 2016-08-31 | 2017-01-04 | 蒋彩云 | Non-oxidizability evaluation methodology based on gold nanorods oxidizing process spectrum change |
| CN108326281B (en) * | 2017-05-17 | 2019-11-12 | 宁波大学 | A kind of dendritic silver nanoparticle wince and its preparation method and application |
| CN110026550A (en) * | 2018-11-07 | 2019-07-19 | 扬州工业职业技术学院 | A kind of nano zero-valence copper and preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56142448A (en) * | 1980-04-09 | 1981-11-06 | Matsushita Electric Ind Co Ltd | Engyme electrode |
| CN102554258A (en) * | 2012-02-03 | 2012-07-11 | 济南大学 | Method for preparing metal silver nanostructure in water solution |
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| JP4750580B2 (en) * | 2006-02-27 | 2011-08-17 | 荏原実業株式会社 | Method and apparatus for measuring ozone concentration |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56142448A (en) * | 1980-04-09 | 1981-11-06 | Matsushita Electric Ind Co Ltd | Engyme electrode |
| CN102554258A (en) * | 2012-02-03 | 2012-07-11 | 济南大学 | Method for preparing metal silver nanostructure in water solution |
Non-Patent Citations (2)
| Title |
|---|
| "H2O2-aided seed-mediated synthesis of silver nanoplates with improved yield and efficiency";Na Li et al.;《Chemical Physics and Physical Chemistry》;20120716;第13卷(第10期);第2527页第二栏第18-33行到2528页第一栏第1-17行及附图2 * |
| "用双还原法制备三角银纳米片及其光学性能";赖文忠等;《物理化学学报》;20100310;第26卷(第4期);第1178页第1.2节 * |
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