CN103160565A - Research for influence on endocytosis of cell transferrins of UNC-51 kinase - Google Patents
Research for influence on endocytosis of cell transferrins of UNC-51 kinase Download PDFInfo
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- CN103160565A CN103160565A CN201110407723XA CN201110407723A CN103160565A CN 103160565 A CN103160565 A CN 103160565A CN 201110407723X A CN201110407723X A CN 201110407723XA CN 201110407723 A CN201110407723 A CN 201110407723A CN 103160565 A CN103160565 A CN 103160565A
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Abstract
The invention discloses research for the influence on the endocytosis of cell transferrins of UNC-51 kinase, wherein maintenance for the stable level of iron elements in cells and body is necessary to the health of life; cell absorption and iron transport are mainly performed via the endocytosis of cell transferrins; the molecular mechanism and the cell mechanism of the endocytosis of cell transferrins are not clear yet; and interpretation for the molecular mechanism and the cell mechanism of the endocytosis of cell transferrins has important significance for prevention and treatment for neurological diseases. The research shows that the COS-7 cells for over-expressing Myc-tagged UNC-51 kinase inhibit the endocytosis of cell transferrins due to that the over-expressed UNC-51 kinase in the COS-7 cells have the function of promoting the dynamic changes of cytoskeletons, thus blocking the recirculation of the complex particles of transferrins, and achieving the effect of preventing the endocytosis of cell transferrins. The research shows the first mode of participating in the endocytosis of cell transferrins of UNC-51 kinase.
Description
Technical field:
The invention belongs to biological chemistry, cytobiology, the fields such as neuroscience have proposed to affect the new mechanism of cell transferrin endocytosis.
Background technology:
Iron (Fe) is that the conformation function of cell and life is necessary, and the maintenance level of keeping ferro element in body is also that life and health is necessary.Cell uptake and transhipment iron are mainly undertaken by the endocytosis of transferrin (transferrin).Although it is relevant that endocytosis and cytoskeleton have, the molecule of the endocytosis of transferrin and celelular mechanism are also not fully aware of.It is dynamic that the UNC-51 kinases directly affects cytoskeleton, makes us think that the UNC-51 kinases also may affect the endocytosis of transferrin (transferrin) in cell.This research confirms that first the UNC-51 kinases has affected the endocytosis of transferrin (transferrin) in cell clearly.
Summary of the invention:
The present invention is intended to express in the kinase whose COS-7 cell of Myc-tagged UNC-51 impact on the transferrin endocytosis with fluorescence microscope.Content of the present invention is:
(1) the kinase whose COS-7 cell of overexpression UNC-51 has suppressed the endocytosis of transferrin
As shown in Figure 1, (A figure shows the cell of red fluorescence), compare with other cells on every side in the kinase whose COS-7 cell of overexpression Myc-tagged UNC-51, and the green fluorescence of transferrin almost cannot see (B figure arrow institute phalangeal cell).This demonstration, the kinase whose COS-7 cell of overexpression Myc-tagged UNC-51 has suppressed the endocytosis to transferrin.
(2) the kinase whose COS-7 cell of overexpression UNC-51 suppresses the pattern of transferrin endocytosis
As shown in Figure 2, the UNC-51 kinases of overexpression can promote the cytoskeleton dynamic to change in the COS-7 cell, thereby has hindered the recirculation of transferrin composite particle precursor, reaches the consequence that encumbers the transferrin endocytosis.This pattern proposes the endocytosis that the UNC-51 kinases has participated in the cell transferrin first.
Description of drawings:
Fig. 1 overexpression UNC-51 kinases and suppressed the COS-7 cell of the endocytosis of transferrin
The pattern of Fig. 2 overexpression UNC-51 kinase inhibition transferrin endocytosis in the COS-7 cell
Embodiment:
The observation of [1] laser scanning fluorescence confocal microscope
DAPI is used for nucleus dyeing, and TexasRed and FITC are respectively used to the dyeing of UNC-51 and tubulin.The ZeissLSM510 confocal microscope is used to cell observation and record.
Claims (3)
- In cell and body, keeping of ferro element maintenance level is the healthy necessary of life.Cell uptake and transhipment iron are mainly undertaken by the endocytosis of transferrin (transferrin).The solution of the molecular cell mechanism of the endocytosis of transferrin bright to nervous system disease prevention and treat significant.This research confirms that first the UNC-51 kinases has affected the endocytosis of transferrin in cell clearly.Its key is found to be:1. the kinase whose COS-7 cell of overexpression UNC-51 has suppressed the endocytosis of transferrin.
- 2. the UNC-51 kinases of overexpression can promote the cytoskeleton dynamic to change in the COS-7 cell, thereby hindered the recirculation of transferrin composite particle precursor, reach the consequence that encumbers the transferrin endocytosis, this pattern proposes the endocytosis that the UNC-51 kinases has participated in the cell transferrin first.
- 3. remember the know-why of binomial requirement in life science, brain science, tumour science, computer science, space science, traffic science, medical science, pharmacy and pharmacopedics, the Chinese medicine science, the therapeutics of various diseases, the application of medical skill and every other aspect and popularization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110407723XA CN103160565A (en) | 2011-12-09 | 2011-12-09 | Research for influence on endocytosis of cell transferrins of UNC-51 kinase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110407723XA CN103160565A (en) | 2011-12-09 | 2011-12-09 | Research for influence on endocytosis of cell transferrins of UNC-51 kinase |
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| CN103160565A true CN103160565A (en) | 2013-06-19 |
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| CN201110407723XA Pending CN103160565A (en) | 2011-12-09 | 2011-12-09 | Research for influence on endocytosis of cell transferrins of UNC-51 kinase |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1190438A (en) * | 1995-06-06 | 1998-08-12 | 转移染色体治疗公司 | Chimeric proteins for use in transport of selected substance into cells |
| WO2006017688A2 (en) * | 2004-08-03 | 2006-02-16 | Biorexis Pharmaceutical Corporation | Combination therapy using transferrin fusion proteins comprising glp-1 |
| CN101231292A (en) * | 2008-02-25 | 2008-07-30 | 中南大学 | Human milk transferrin and application of detection kit thereof in nasopharyngeal carcinoma diagnosis |
| CN101325965A (en) * | 2005-12-09 | 2008-12-17 | 微博有限公司 | Use of transferrins for treating destructive inflammation of mucous membrane |
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2011
- 2011-12-09 CN CN201110407723XA patent/CN103160565A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1190438A (en) * | 1995-06-06 | 1998-08-12 | 转移染色体治疗公司 | Chimeric proteins for use in transport of selected substance into cells |
| WO2006017688A2 (en) * | 2004-08-03 | 2006-02-16 | Biorexis Pharmaceutical Corporation | Combination therapy using transferrin fusion proteins comprising glp-1 |
| CN101325965A (en) * | 2005-12-09 | 2008-12-17 | 微博有限公司 | Use of transferrins for treating destructive inflammation of mucous membrane |
| CN101231292A (en) * | 2008-02-25 | 2008-07-30 | 中南大学 | Human milk transferrin and application of detection kit thereof in nasopharyngeal carcinoma diagnosis |
Non-Patent Citations (1)
| Title |
|---|
| HUAIZE TIAN: "Overexpression of Conserved Kinase UNC-51 Inhibits the Transferrins Endocytosis into the Mammalian Cells", 《ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS》, vol. 15, 31 December 2009 (2009-12-31), pages 347 - 349 * |
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Application publication date: 20130619 |