CN103141685A - Mycotoxin detoxication agent for swine feed - Google Patents
Mycotoxin detoxication agent for swine feed Download PDFInfo
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Abstract
The invention provides a compound mycotoxin detoxication agent for a swine feed, which is prepared by combining dandelion extract, clove extract, alpinia galangal extract, superfine ground purslane herb, superfine ground pepper, superfine ground cinnamon, fine ground smoked plum, superfine ground ligusticum wallichii, superfine ground rhizoma curcumae longae and organically modified montmorillonite. Raw materials of the compound mycotoxin detoxication agent are selected and obtained according to the plant species about homology of medicine and food of the National Ministry of Health, and thus the compound mycotoxin detoxication agent has the characteristics of wide source of the raw materials, high bioavailability and no toxicity and residue. The invention provides the compound mycotoxin detoxication agent which is capable of inhibiting propagation of mycetes and absorbing mycotoxin, and is also capable of effectively removing the damage of mycotoxin to the growth performance, the production performance and the health condition to animals.
Description
Technical field
The present invention relates to the detoxicating mycotoxin agent of a kind of pig feed.Additive of the present invention can effectively be removed mycotoxin to the harm of growth of animal performance, production performance and health status, belongs to the feed additive field that ensures feed safety.
Background technology
Mycotoxin is the poisonous secondary metabolite that some mould produces in the growth and breeding process on cereal or feed, has had up to now 300 kinds of mycotoxins of surpassing to be isolated and identified out.During herding is produced, common and mycotoxin that can directly affect growth of animal production performance and health status is mainly produced by four kinds of mould Pseudomonas: Eurotium (mainly secreting aflatoxin, ochratoxin etc.), Penicillium (mainly secreting the mould toxin of tangerine), Claviceps (mainly secreting ergotoxin), fusobacterium (mainly secreting vomitoxin, zearalenone, T-2 toxin, fumonism etc.).Mycotoxin can cause that organa parenchymatosum's damage (liver, kidney), immunosupress appear in pig, (sow false heat or oestrous cycle extend breeding difficulty, after ablactation, insemination of sows returns the rising of feelings rate, Repeat breeding or pseudopregnancy occurs, in-pig miscarriage or product stillborn foetus, milking sow lactation amount reduces or agalasisa, the boar sexual desire is poor, degradation under sperm motility), feed intake decline, the stagnation of growing, become thin, the illness such as feed conversion rate reduction.
Cereal (feedstuff) thus field growing, results, storage, process and the process of feeding in all might fungal infection cause the generation of mycotoxin.The harm of mycotoxins in feed has been serious problems of the global aquaculture of impact.According to FAO (Food and Agriculture Organization of the United Nation) investigation, the whole world is accounted for 25% left and right of its total amount every year by the cereal of mould contamination, oilseed and feed.2006～2007 years, Ao Teqi company found more than 800 sample detection that Asia gathers, and zearalenone, the deoxidation melon of raw food, feed etc. withers the positive rate of reaping hook alcohol, fumonism, aflatoxin all more than 90%.Ao Zhi has just waited 225 sample detection has been found; corn, complete feed, protein feed, corn byproducts (corn germ cake, corn protein powder, maize alcohol stillage etc.) milk cow mixed fodder; 6 kinds of mycotoxin recall rate average out tos 93.2% of ensilage; the highest vomitoxin reaches 99.1%; exceeding standard rate average out to 29.6%, the highest vomitoxin that exceeds standard reaches 53.5%.The Ministry of Agriculture was to coming from 32 corn samples in the whole nation in 2009,46 protein raw materials samples, 23 complete feed samples detect discovery, aspergillus flavus toxin pollutes the most serious, zearalenone the second, T-2 toxin, vomitoxin, FT, ochratoxin pollute ubiquity.All samples all detects aspergillus flavus toxin and vomitoxin, 89% corn sample is the zeranol positive reaction, and usually by mycotoxin contamination more than three kinds or three kinds, 80% dregs of beans contains aspergillus flavus toxin, T-2 toxin and three kinds of toxin of zearalenone simultaneously.2011, hundred Ao Ming companies are to having carried out the detection of 13854 samples time from all over the world 4327 samples, each mycotoxin positive rate is respectively: aflatoxin 27%, zearalenone 40%, vomitoxin 59%, FT 51%, ochratoxin 27%.
By rejecting mouldy or damaged seed, slough and contain the more outer covering of a kernel of toxin or plumule, clear water elutriation, the methods such as sunlight insolation, radiation, heating, can reduce the toxicity of mould quantity and reduction mycotoxin, but the method is difficult to thoroughly eliminate the toxin that mould produces.Multiple natural or synthetic mycotoxin scavenger had appearred on market in recent years, with removing mould and the mycotoxin that has polluted in feed and raw material, by its mode of action, can be divided into following a few class: physics detoxicity method, chemical detoxication method, biological detoxication method etc. are several.
(1) physics detoxification: its principle is can the absorbing mycotoxin material by adding in feed, and toxin is not absorbed by animals through animal intestinal the time, directly excretes.Mold toxin sorbent commonly used has, natural aluminosilicate (as zeolite, montmorillonite, diatomite, kaolin etc.), mannan-oligosaccharides etc.
Natural aluminosilicate to the adsorption capacity of mycotoxin not only with adsorbent relevant with the size of total charge dosage, charge distribution, hole and available surface area (specific area), and be decided by the physical features such as polarity, solubility, molecular size, molecular shape, CHARGE DISTRIBUTION of the lps molecule that is adsorbed.Natural aluminosilicate has advantages of larger specific area, but simultaneously also has certain defective: show as that absorption affinity is little and efficient is low; Must heavy dose of (1-3%) add ability effectively in use, and only for aflatoxin and ergotin, certain absorption affinity be arranged; To vomitoxin, the T-2 toxin, zearalenone, ochratoxin A, snakelike verticillium toxin, Festuca Arundinacea toxin etc. does not have suction-operated; Nutriment in feed also there is suction-operated, thereby reduces feed nutrition concentration; Use the feed of this kind adsorbent to exist by the risk of dioxin (dioxins) and heavy metal pollution.
Mannan-oligosaccharides prevent the animal mycotoxin poisoning aspect,, effect few with its addition is significantly and cause people's extensive attention in conjunction with characteristics such as the mycotoxin kind are wide.Present studies show that in a large number, mannan-oligosaccharides can with many mycotoxin combinations, eliminate mould to the impact of body.The simulation broiler chicken alimentary canal in vitro tests such as Raju show, mannan-oligosaccharides is respectively 82.5%, 51.6% and 26.4% to AFB1, FT toxin, ochratoxin in conjunction with rate.The in vitro test of Trenholm etc. shows, under the pH=4.0 condition, mannan-oligosaccharides reaches respectively 80%, 95%, 77%, 59% and 12% to the knot platform rate of zearalenone, aflatoxin, zearalenone, fumonisin and vomitoxin.Mannan-oligosaccharides is the oligosaccharide kind material that mainly extracts from the yeast cell wall skin, and therefore obtaining mannan-oligosaccharides needs tightly high fractionation technique accurately.Domestic shortage is with the yeast cell wall demixing technology, just simply yeast cell wall integral body is pulverized and used as immunostimulant and mould adsorbant function product, cause mannan-oligosaccharides active ingredient dosage on the low side and make the absorbing mycotoxin effect differ greatly with the result of document report, limited greatly promoting the use of in market.
(2) chemical detoxication.Some mycotoxin can be destroyed degraded by the chemicals of part, with the mouldy seed of soda lye wash, soaks moldy feed with ammoniacal liquor or copper-bath as common.But there is following drawback in the method: different chemical substances often only effectively can not be degraded to other toxin to certain toxin, and the chemical substance of use is residual and produce new poisonous and harmful effect in feed.(3) biological detoxication.Mainly contain enzymatic isolation method and microbe fermentation method, enzymatic isolation method is to make the functional group of mycotoxin/toxicity inactivation by enzyme digestion reaction.The limiting factor that the method is subject to has, and in essence, enzyme is also protein, and its vigor can be subjected to pH, temperature and be subjected to the impact of matter (mycotoxin).The defective of the method is: feed may not necessarily provide the environment that is fit to this kind enzyme; Enzyme is very high to the narrow spectrum degree of the selection that is subjected to matter, be critical sometimes, so these class methods equally only has certain effect to certain toxin; Secondary species after enzymolysis might be toxic; Enzyme is thermo-labile, at the easy inactivation of feed process.The effect of microbe fermentation method is slow, is not suitable for feed factory and plant.Therefore, use that biological detoxication method cost is higher and effect is unstable, thus in production practices still difficulty apply.
(4) normal flora and Chinese herbal medicine detoxicity method.In recent years, having and add lactic acid bacteria with the report that removes aflatoxin in feed, is mainly by keeping the gut flora balance, strengthening the immunity of body to alleviate the harm of toxin.The method contratoxin does not have specificity, only rests on the experimental stage, differs greatly from practical application.
Since 2007, detoxicating mycotoxin agent commodity all emerge in an endless stream every year, but annual feed mould Positive rate, the poisoning incidence of disease of livestock and poultry mycotoxin remain high, and from the angle of actual production practice, existing detoxicating mycotoxin agent effect on market is undesirable.
Drawback for single detoxicating mycotoxin agent appearance, " catalogue of integration of drinking and medicinal herbs " that the inventor announces according to the Ministry of Public Health, be food and medicine material, can be used for filtering out the strong natural plants of several antifungi effects in the material of health food, and then with the composite composite mold toxin detoxifying agent of making of organic modification montmorillonite, reach the purpose of removing Mycotoxins in Feed from the angle that mould fungus inhibition is bred and absorbing mycotoxin is worked along both lines.
Summary of the invention
The present invention is according to the floristic regulation of health ministry about integration of drinking and medicinal herbs, filter out the strong natural organic plants of antifungi effect such as Chinese cassia tree, cloves, Chinese prickly ash, galangal, dark plum, Ligusticum wallichii, turmeric, purslane, dandelion, by reasonable processing and prescription, and then with the montmorillonite reasonable formula after organic modification, final purpose be to provide a kind of can mould fungus inhibition breeding, composite mold toxin detoxifying agent that again can absorbing mycotoxin.
Technical scheme of the present invention be with dandelion extract, Flos Caryophylli extract, galangal rhizome extract, purslane herb Ultramicro-powder mince, the Chinese prickly ash Ultramicro-powder minces, the Chinese cassia tree Ultramicro-powder minces,, the dark plum micro mist minces, the Ligusticum wallichii Ultramicro-powder minces, the turmeric Ultramicro-powder minces into the Main Function thing, by suppressing or killing mould common in feed and play the effect of eradicating the mycotoxin source from the source; Take the montmorillonite of organic modification as the secondary role thing, adsorb the effect of playing mycotoxin in adsorption feed by physical property.This compound composite mold toxin detoxifying agent makes an addition in swine feed, can reach aflatoxin, FT, vomitoxin, zearalenone, the effective scavenging action of T-2 toxin.
The invention provides a kind of can mould fungus inhibition breeding, composite mold toxin detoxifying agent that again can absorbing mycotoxin, it is by various plants micro mist and plant extracts, a kind of organic modification montmorillonite proportioning forms.
The invention provides a kind of composite mold toxin detoxifying agent, it forms (each amounts of components is weight portion) by following component
The montmorillonite 35～45 of organic modification
Dandelion extract 5～10
Flos Caryophylli extract 5～10
Galangal rhizome extract 5～15
Purslane herb Ultramicro-powder minces 15～20
The Chinese prickly ash Ultramicro-powder minces 10～15
The Chinese cassia tree Ultramicro-powder minces 20～30
The Ligusticum wallichii Ultramicro-powder minces 15～25
The turmeric Ultramicro-powder minces 20～25
In a preferred embodiment of the invention, the invention provides a kind of consist of (each amounts of components is weight portion) of composite mold toxin detoxifying agent:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
Purslane herb Ultramicro-powder minces 20
The Chinese prickly ash Ultramicro-powder minces 10
The Chinese cassia tree Ultramicro-powder minces 20
The Ligusticum wallichii Ultramicro-powder minces 15
The turmeric Ultramicro-powder minces 20
Detoxification experimental results show that: organic composite type detoxicating mycotoxin of the present invention agent is added by 0.1～0.4 kilogram/100 kilograms batches, can reduce the poisoning symptom of degradation mycotoxin under swinery false heat, watery stools, expiratory dyspnea, food refusal or feed intake.
Therefore, the invention provides composite mold toxin detoxifying agent of the present invention improve organa parenchymatosum damage (liver, kidney), immunosupress, breeding difficulty, feed intake decline, the stagnation of growing, become thin, purposes aspect the illness such as feed conversion rate reduction.
In a preferred embodiment of the invention, described animal is the pig of each age level.Addition in feed is 0.15 kilogram/100 kilograms complete diet pellets.
On the other hand, the invention provides the preparation method of a kind of organic composite type detoxicating mycotoxin agent.The method comprises the following steps:
(1) extraction of montmorillonite and organic modification
Purifying accurately takes 100g days bentonites and 4g sodium carbonate, is placed in the 2000ml beaker, adds 900ml distilled water and 3～l0ml, 10% sodium hexametaphosphate solution dispersant, makes 10% suspension; Change in high speed dispersor, 3000r/min constantly stirs 1h; Be placed in the centrifugal l～10min of centrifuge 3000r/min, remove the thicker sediment in bottom; Be diluted with water l and doubly be placed in the high-speed homogenization machine and disperse 30～60min, centrifugal 1～5min removes top particulate and clear liquid, then centrifugal dehydration 30～60min, gets dry 24h under l05 ℃ of bottom precipitation thing, and grinding is sieved, and screenings is standby, namely makes na-montmorillonite.
Add a certain amount of na-montmorillonite, softex kw, appropriate distilled water in the 500ml flask, and dripping a small amount of dilute sulfuric acid, to adjust the pH value be 3, be put in heating stir about 2h in the 60X2 water bath with thermostatic control, it is fully exchanged, will be after the montmorillonite slip centrifugation at normal temperatures after intercalation with the distilled water washing more than 3 times, can't detect Br-to eluate; 40 ℃ of lower drying under reduced pressure, 105 ℃ of activation 30min are crushed to 200 orders with polishing, are placed in drier and preserve, and namely make organically modified montmorillonite clay.
(2) preparation of purslane micro mist: get the purslane herb, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
(3) the Chinese prickly ash Ultramicro-powder preparation of mincing: get the Chinese prickly ash dry mature fruit, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
(4) the Chinese cassia tree Ultramicro-powder preparation of mincing: get the dry bark of Chinese cassia tree, impurity elimination, grind into powder.Collect the powder by 100 mesh sieve sheets.
(5) the Ligusticum wallichii Ultramicro-powder preparation of mincing: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
(6) the turmeric Ultramicro-powder preparation of mincing: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
(7) dandelion herb extract: commercial, require general flavone content greater than 5%.
(8) Flos Caryophylli extract: commercial, require to contain volatile oil 16～19%.
(9) galangal rhizome extract: commercial, require extract powder, 10:1.
(10) material that above-mentioned steps is obtained ratio by weight mixes.
The montmorillonite 35～45 of organic modification
Dandelion extract 5～10
Flos Caryophylli extract 5～10
Galangal rhizome extract 5～15
Purslane herb Ultramicro-powder minces 15～20
The Chinese prickly ash Ultramicro-powder minces 10～15
The Chinese cassia tree Ultramicro-powder minces 20～30
The Ligusticum wallichii Ultramicro-powder minces 15～25
The turmeric Ultramicro-powder minces 20～25
In a preferred embodiment of the invention, wherein the ratio by weight described in step (10) is mixed into:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
Purslane herb Ultramicro-powder minces 20
The Chinese prickly ash Ultramicro-powder minces 10
The Chinese cassia tree Ultramicro-powder minces 20
The Ligusticum wallichii Ultramicro-powder minces 15
The turmeric Ultramicro-powder minces 20
Advantage of the present invention
Composite mold toxin detoxifying agent of the present invention is the floristics of selecting the integration of drinking and medicinal herbs that the Ministry of Public Health issues, form through Crushing of Ultrafine or extraction, the processes such as concentrated, have that raw material sources are extensive, bioavailability is high, the characteristics of nontoxic, noresidue, the organic modification of montmorillonite has improved adsorption capacity and has widened the absorption spectrum.These organic substances composite, provide a kind of can be from mould fungus inhibition breeding on the source, composite mold toxin detoxifying agent that again can absorbing mycotoxin has improved pig growth performance, production performance, health status.
Below in conjunction with embodiment, the specific embodiment of the present invention is described further.Following examples only are used for explanation the present invention, but are not used for limiting the scope of the invention.
Embodiment
Preparation and the organic modification of embodiment 1 montmorillonite
Purifying accurately takes 100g days bentonites and 4g sodium carbonate, is placed in the 2000ml beaker, adds 900ml distilled water and 3～l0ml, 10% sodium hexametaphosphate solution dispersant, makes 10% suspension; Change in high speed dispersor, 3000r/min constantly stirs 1h; Be placed in the centrifugal l～10min of centrifuge 3000r/min, remove the thicker sediment in bottom; Be diluted with water l and doubly be placed in the high-speed homogenization machine and disperse 30～60min, centrifugal 1～5min removes top particulate and clear liquid, then centrifugal dehydration 30～60min, gets dry 24h under l05 ℃ of bottom precipitation thing, and grinding is sieved, and screenings is standby, namely makes na-montmorillonite.
Add a certain amount of na-montmorillonite, softex kw, appropriate distilled water in the 500ml flask, and dripping a small amount of dilute sulfuric acid, to adjust the pH value be 3, be put in heating stir about 2h in the 60X2 water bath with thermostatic control, it is fully exchanged, will be after the montmorillonite slip centrifugation at normal temperatures after intercalation with the distilled water washing more than 3 times, can't detect Br-to eluate; 40 ℃ of lower drying under reduced pressure, 105 ℃ of activation 30min are crushed to 200 orders with polishing, are placed in drier and preserve, and namely make organically modified montmorillonite clay.
The preparation that embodiment 2 Plant Powders mince
The preparation of purslane micro mist: get the purslane herb, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
The preparation that the Chinese prickly ash Ultramicro-powder minces: get the Chinese prickly ash dry mature fruit, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
The preparation that the Chinese cassia tree Ultramicro-powder minces: get the dry bark of Chinese cassia tree, impurity elimination, grind into powder.Collect the powder by 100 mesh sieve sheets.
The preparation that the Ligusticum wallichii Ultramicro-powder minces: get the dry rhizome of Ligusticum wallichii, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
The preparation that the turmeric Ultramicro-powder minces: get the dry rhizome of turmeric, impurity elimination, grind into powder.Collect the micro mist by 100 mesh sieve sheets.
Acquisition and the standard of embodiment 3 plant extracts
The dandelion herb extract: be purchased from Xi'an grass roots bioengineering Co., Ltd, general flavone content is greater than 5%.
Flos Caryophylli extract: be purchased from the three-dimensional Bioisystech Co., Ltd in Xi'an, contain volatile oil 16～19%.
Galangal rhizome extract: be purchased from sharp source, Xi'an bio tech ltd, the 10:1 extract.
Embodiment 4 composite mold toxin detoxifying agent preparations
The montmorillonite of the organic modification of embodiment 1 preparation is pressed following weight portion proportioning with the plant plant extracts that embodiment 3 obtains:
The montmorillonite 40 of organic modification
Dandelion extract 10
Flos Caryophylli extract 10
Galangal rhizome extract 10
The abundant mixing of above-mentioned each component obtains mixture A.
The plant micro mist of embodiment 2 preparations is pressed following weight portion proportioning:
Purslane herb Ultramicro-powder minces 20
The Chinese prickly ash Ultramicro-powder minces 10
The Chinese cassia tree Ultramicro-powder minces 20
The Ligusticum wallichii Ultramicro-powder minces 15
The turmeric Ultramicro-powder minces 20
With the abundant mixing of each component, obtain mixture B
Mixture A and mixture B are fully mixed, namely obtain this composite mold toxin detoxifying agent
The embodiment 5 adsorbing extracorporeal simulating experiments of composite mold toxin detoxifying agent
1. experimentation
(1) get 3 sizes, internal diameter, highly consistent white transparent water tumbler, be filled with water to 4/5 place; Be numbered respectively 1,2,3
(2) drip three Parker inks (simulation mycotoxin) with suction pipe in 1 water tumbler to being numbered.
(3) get two vitamin B2s (human represents nutriment), fully put into after grinding and be numbered 2 cup, stir and make aqueous solution yellowly.
(4) No. 3 cup does not add anything cup in contrast.
(5) the composite mold toxin detoxifying agent that adds respectively 2g to detect in three cups.
(6) each beaker stirred 5 minutes with glass bar, saw result after standing 40 minutes.2. experimental result and conclusion
It is colourless that No. 1 water tumbler solvent presents, and a cup end solute is black, and No. 2 cup remains yellow, and No. 3 cup nondiscolourings illustrate that this composite mold toxin detoxifying agent does not adsorb nutrition in absorbing mycotoxin.
The inhibitory action of embodiment 6 composite mold toxin detoxifying agents to mould
For representing mould, observe composite mold toxin detoxifying agent of the present invention to the inhibitory action of mould with aspergillus flavus.
(1) add composite mold toxin detoxifying agent of the present invention on Czapek's medium (CA) basis.
Concrete steps are: take sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate (MgSO47H2O) 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, 2 gram composite mold toxin detoxifying agents, distilled water 1000mL, heating for dissolving is poured the mycotoxin liquid 2ml of new preparation into when being cooled to 60 ℃ of left and right, then pour mixed liquor into glass culture dish.
(2) in 25～28 ℃ of cultivations, observed result after 3-5 days.
Result and conclusion
Do not see that fungus growth is arranged, illustrate that organic composite type detoxicating mycotoxin of the present invention agent has good mould fungus inhibition effect.
Embodiment 7 organic composite type detoxicating mycotoxin agent are measured the mycotoxin adsorption ability
This experiment is gastrointestinal tract environment in analogue body, the absorption situation of this organic composite type detoxicating mycotoxin agent to AFB1, zearalenone, vomitoxin, FT and T-2 toxin that adopted the two-phase method system evaluation.
1. the preparation of mycotoxin titer and adsorption test
The preparation of (1) 0.1 mol/L PBS (pH 4.0 and pH 6.8)
Precision takes in the distilled water of 35.814 g disodium hydrogen phosphate dodecahydrate to 900 mL, then uses phosphorus acid for adjusting pH value to 4.0 and 6.8, and is standby after constant volume.
(2) standard mycotoxin working solution (pH 3.0)
Limit standard national according to Mycotoxins in Feed, and the pig amount of drinking water is 2-3 times of feed intake.The content of determining mycotoxin in working solution is as follows respectively:
AFB1 is 150ppb, and zearalenone is 50ppb, and vomitoxin is 5ppm, and the T-2 toxin is 0.5ppm, and FT is 50ppb.
(3) adsorption test in adsorption test 1(simulation stomach, PH=4.0)
Take 0.75mg mold toxin sorbent sample in test tube, add l L pH3.0 mycotoxin working solution, in system, the addition of mold toxin sorbent is 0.75 mg/L.Blank is not for adding the 1L pH3.0 mycotoxin working solution of any mold toxin sorbent.Get above-mentioned working solution 10ml, put into respectively a test tube, test tube is placed on 37 ℃ of constant temperature oscillators, concussion 1 h.After reaction finishes, in centrifugal 10 min of 3 500 r/m, the ELISA method is measured the mycotoxin content in supernatant.
(4) adsorption test in adsorption test 2(simulation small intestine, PH=6.8)
After above-mentioned experiment finished, supernatant in the centrifuge tube that inclines added 10 mL pH6.8 phosphate buffers in residue, 37 ℃ of isothermal vibration 1 h.After reaction finishes, in centrifugal 10 min of 3 500 r/m, the ELISA method is measured remaining mycotoxin content in supernatant.
(5) according to the content that detects toxin, calculate the adsorption efficiency of mold toxin sorbent by following formula.
Y=100×（A-B-C）/A
In following formula: Y represents that mold toxin sorbent is to the adsorption efficiency of mycotoxin; A represents blank group (being not add adsorbent in the mycotoxin working solution) content; B represents in adsorption test 1 content of mycotoxin in supernatant; C represents in desorption test 2 content of mycotoxin in supernatant.
2. result of the test and conclusion
The agent of this organic composite type detoxicating mycotoxin is to aflatoxin, zearalenone, vomitoxin, and the adsorption rate of T-2 toxin, FT is respectively 92.3%, 86.7%, 85.4%, 72.3%.78.4%, illustrate that composite mold toxin detoxifying agent of the present invention can effectively adsorb above-mentioned mycotoxin.
Embodiment 8 detoxification experiments
The preparation of test daily ration and experimental animal
(1) preparation corn-soybean meal complete feed, corn-soybean meal is without mildew phenomena, as daily ration 1
(2) preparation Corn-soybean-peanut meal type complete feed, corn, dregs of beans, peanut meal have obvious mildew phenomena, as daily ration 2.
(3) get part daily ration 2, add wherein composite mold toxin detoxifying agent of the present invention, dosage is 0.15 kilogram/100 kilograms complete feeds, as daily ration 3.
Every nutritive index of above-mentioned three daily rations is identical.
(4) get 60 sizes and the identical pig of genetic background, be equally divided into three groups, 20 every group, compiling respectively is three groups of A, B, C, and the weight average of A group is 60.5 kilograms, and the average weight of B group is 61.8 kilograms, and the average weight of C group is 61.5 kilograms.A organizes the daily ration 1 of feeding, and B organizes the daily ration 2 of feeding, and C organizes the daily ration 3 of feeding, feed period 20 days.
2. result of the test and conclusion
After A group feed period finishes, average weight is 78.8 kilograms, and any symptom does not appear in swinery; After the B group is fed and finished, average weight is 78.5 kilograms, has two pig the diarrhoea phenomenon to occur; After the C group is fed and finished, average weight is 70.3 kilograms, and the pig uniformity is poor, and the severe diarrhea phenomenon appears in 65% pig, and ight soil is water sample, spurting; Severe dyspnea appears in 45% pig, and the vulva redness appears in 35% pig, and all pig occurs by the in disorder phenomenon of hair, and feed intake obviously reduces.
Result of the test explanation detoxifying agent of the present invention is absorbing mycotoxin effectively, reduces immunosupress, false heat, feed intake that mycotoxin brings slow, the clinical symptoms such as become thin that descend, grow.
Claims (4)
1. pig feed detoxicating mycotoxin agent, its montmorillonite proportioning by various plants micro mist, plant extracts and organic modification forms.
2. detoxicating mycotoxin agent claimed in claim 1, it consists of:
Wherein the consumption of each component is weight portion.
3. detoxicating mycotoxin agent claimed in claim 2, it consists of:
Wherein the consumption of each component is weight portion.
4. in claim 1～3, the described pig feed of any one uses the detoxicating mycotoxin agent in the purposes of mould fungus inhibition breeding and absorbing mycotoxin.
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| CN104799017A (en) * | 2014-01-24 | 2015-07-29 | 内蒙古润隆化工有限责任公司 | Preparation method of modified montmorillonite powder for feeds |
| CN104855691A (en) * | 2015-06-15 | 2015-08-26 | 江苏益客食品有限公司 | Anti-mycotoxin feed additive for ducks as well as preparation method and application of anti-mycotoxin feed additive |
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| CN107927338A (en) * | 2017-11-27 | 2018-04-20 | 山东迅达康兽药有限公司 | A kind of medicine, its preparation method and the application of anti-mildew verticillium toxin |
| CN108014126A (en) * | 2017-12-15 | 2018-05-11 | 合肥中龙神力动物药业有限公司 | A kind of biology takes off mycin and its production method |
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| CN108740362A (en) * | 2018-06-25 | 2018-11-06 | 宿松县华图生物科技有限公司 | A kind of biological feedstuff improving disease resistance |
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