CN103141412B - Method for identifying wild population and cultured population of parent crabs of eriocheir sinensis based on morphological parameters - Google Patents
Method for identifying wild population and cultured population of parent crabs of eriocheir sinensis based on morphological parameters Download PDFInfo
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- CN103141412B CN103141412B CN201210364189.3A CN201210364189A CN103141412B CN 103141412 B CN103141412 B CN 103141412B CN 201210364189 A CN201210364189 A CN 201210364189A CN 103141412 B CN103141412 B CN 103141412B
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Abstract
The invention discloses a method for identifying a wild population and a cultured population of parent crabs of eriocheir sinensis based on morphological parameters. Measurement is made on the back face of carapace and an ambulatory leg. The method is characterized in that morphological measurement parameters of female crabs are L2, L4, L6, F15 and F44, and the morphological measurement parameters of male crabs are L2, L15, F11, F33 and F44; an identification formula, D=66.613L2+31.203L4-43.969L6-159.018F15+34.628F44+2.278, of the female crabs is created by using the L2, L4, L6, F15 and F44; an identification formula, D=83.669L2-70.977L15-26.518F11+72.540F33+44.416F44+26.146, of the male crabs is created by using the L2, L15, F11, F33 and F44; when D is greater than 0, the population is the wild population; and when D is less than 0, the population is the cultured population.
Description
Technical field
The invention belongs to technical field of aquaculture, the discriminating of especially a kind of ParentEriocheir sinensis wild population and cultured population.
Background technology
Eriocheir sinensia (Eriocheir sinensis) is commonly called as river crab, is under the jurisdiction of Arthropoda, Crustachia, and Decapoda, Eriocheir, delicious, nutritious because of meat flavour, be loved by the people.Eriocheir sinensia is widely distributed, at China's Bohai and Yellow Seas and all provinces of East China Sea lefteye, all has distribution, and colony's natural distribution be take the middle and lower reach of Yangtze River as main.The Eriocheir sinensia of different water systems exists certain different in form.For example, the 4th step prosthomere of Eriocheir sinensis in ChangJiang River is compared with long and narrow, and the 4th step prosthomere of the Oujiang River water system Eriocheir sinensia is compared with short and wide, and the step of Liaohe River water system Eriocheir sinensia is shorter.
Morphological method is by the morphological differences between colony, to differentiate the conventional method of population, is also the main contents of Research on Genetic Variation in early stage classical genetics.There are reports by morphological method, to study the research of Population of Eriocheir Sinensis difference between the different water systems in various places.For example, He Jie etc., by the analysis to outside 54 morphological characters of the Eriocheir sinensia of two water systems of the Oujiang River and the Liaohe River, obtain both and have obvious geographic isolation phenomenon; The difference that Li Chenhong etc. find the Population of Eriocheir Sinensis of northern water system and the Eriocheir of southern water system by morphological analysis is more than subspecies level of difference.Above the Research on differences of Eriocheir sinensia is all concentrated on to the comparative study between different water system populations, its objective is the geographical difference between population.Because there is certain difficulty, by morphology, differentiate that the morphological differences of same water system-the Changjiang river Eriocheir sinensia wild population and cultured population also has no research report always.Due to the Changjiang river Eriocheir sinensia wild population delicious meat, expensive, the phenomenon of passing a fake product off as a genuine one on market happens occasionally, and many Changjiang river culture of Chinese mitten crab colonies that propagate artificially mix wherein, cause Eriocheir sinensia market more chaotic.Invent a kind of authentication technique and differentiate Eriocheir sinensia wild population and cultured population, this is the needs of differentiating Eriocheir sinensia parent in breeding production, be also existing market in the urgent need to.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of method of differentiating ParentEriocheir sinensis wild population and cultured population, for differentiating in breeding production that Eriocheir sinensia parent provides a kind of simple and feasible authentication technique, prevent that the ParentEriocheir sinensis of propagating artificially from pretending to be the wild close crab in the Changjiang river to sell simultaneously.
Technical scheme of the present invention adopts the measurement point of the Eriocheir sinensia carapace back side and step place: interior volume tooth A or A ', outer volume tooth B or B ', F or F ' at the bottom of E or E ' the 3rd side tooth at the bottom of eyeground C or C ' the first side tooth D or D ' the second side tooth, G or G ' at the bottom of the 4th side tooth, the wide outermost point HH ' of body, trailing edge circle angle I or I ', J in trailing edge, K in neck ditch, L incises in central authorities, AA ' on line middle part L ', step meropodium site M, N, step propodite site O, P, step dactylus site P, Q, and adopt following parameter-definition: L1-AA ', L2-BB ', L3-CC ', L4-DD ', L5-EE ', L6-FF ', L7-GG ', L8-II ', L9-JL ', L10-JL, L11-KL, L12-KJ, L13-IL, L14-CI, L15-EI, L16-FI, L17-GI, L18-HH ', F11, F21, F31, the MN of F41-1～4 step, F12, F22, F32, F42-the 1st OP to the 4th step, F13, F23, F33, propodite the widest part of F43-the 1st to the 4th step is wide, F14, F24, F34, F44-the 1st PQ to the 4th step, F15, F25, F35, dactylus the widest part of F45-the 1st to the 4th step is wide, wherein: L-carapace form variable, F-step form variable, H-height form variable, it is characterized in that differentiating that operating procedure is as follows:
(1) differentiate after close crab male and female sex, first adopt slide measure to choose morphological parameters measurement point at the Eriocheir sinensia carapace back side and 1st～4 steps place, every close crab is measured filter out 5 morphological parameters data, male and female are carried out respectively record, it is L2, L4, L6, F15, F44 that female crab is specifically measured site, and it is L2, L15, F11, F33, F44 that male crab is specifically measured site;
(2) for female crab, 5 morphological parameters L2, the L4, L6, F15, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of female crab of setting up Eriocheir sinensia wild population and cultured population is as follows:
D=66.613L2+31.203L4-43.969L6-159.018F15+34.628F44+2.278；
(3) for male crab, 5 morphological parameters L2, the L15, F11, F33, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of male crab of setting up Eriocheir sinensia wild population and cultured population is as follows:
D=83.669L2-70.977L15-26.518F11+72.540F33+44.416F44+26.146；
(4) for correcting the impact of Eriocheir sinensis body specification difference on morphological feature value, by 5 measured morphological feature values all divided by the wide value of body of its corresponding close crab, using the eigen value of processing as standard value;
(5), by after the female crab to be identified morphological feature value standardization corresponding with male crab, the female crab of substitution and male crab are differentiated in formula respectively: when D>0, be Eriocheir sinensia wild population; During D<0, it is Eriocheir sinensis colony.
Outstanding feature of the present invention is by measuring each 5 morphological parameters of male and female parent crab, can distinguishing fast Eriocheir sinensia wild population and cultured population.Differentiate that rate of accuracy reached is more than 92%.Have easy and simple to handle, fast, feature accurately, can save manpower and financial resources, close crab not damaged is differentiated, in breeding production and market sale, all tool is easy to the remarkable result of popularization.
Accompanying drawing explanation
Measurement site, Fig. 1 Eriocheir sinensia carapace back side schematic diagram;
Site schematic diagram is measured in the step of Fig. 2 Eriocheir sinensia.
Embodiment
The specific embodiment of the present invention adopts the measurement point of the Eriocheir sinensia carapace back side and step place: interior volume tooth A or A ', outer volume tooth B or B ', eyeground C or C ', the first side tooth D or D ', E or E ' at the bottom of the second side tooth, F or F ' at the bottom of the 3rd side tooth, G or G ' at the bottom of the 4th side tooth, the wide outermost point HH ' of body, trailing edge circle angle I or I ', J in trailing edge, K in neck ditch, L incises in central authorities, AA ' on line middle part L ', step meropodium site M, N, step propodite site O, P, step dactylus site P, Q, and adopt following parameter-definition: L1-AA ', L2-BB ', L3-CC ', L4-DD ', L5-EE ', L6-FF ', L7-GG ', L8-II ', L9-JL ', L10-JL, L11-KL, L12-KJ, L13-IL, L14-CI, L15-EI, L16-FI, L17-GI, L18-HH ', F11, F21, F31, the MN of F41-1～4 step, F12, F22, F32, F42-the 1st OP to the 4th step, F13, F23, F33, propodite the widest part of F43-the 1st to the 4th step is wide, F14, F24, F34, F44-the 1st PQ to the 4th step, F15, F25, F35, dactylus the widest part of F45-the 1st to the 4th step is wide, wherein: L-carapace form variable, F-step form variable, H-height form variable, concrete discriminating operating procedure is as follows:
Close crab is differentiated after male and female, first adopt slide measure to locate to choose measurement point (referring to accompanying drawing) at the Eriocheir sinensia carapace back side and 1st～4 steps etc., every close crab selects 5 length data to measure, length is accurate to 0.01mm, after finishing, corrects each DATA REASONING slide measure one time, male and female are carried out respectively record, and the concrete measurement parameter of female crab is L2, L4, L6, F15, F44, and the concrete measurement parameter of male crab is L2, L15, F11, F33, F44;
For female crab, 5 morphological parameters L2, the L4, L6, F15, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of female crab of setting up Eriocheir sinensia wild population and cultured population is as follows:
D=66.613L2+31.203L4-43.969L6-159.018F?15+34.628F44+2.278；
For male crab, 5 morphological parameters L2, the L15, F11, F33, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of male crab of setting up Eriocheir sinensia wild population and cultured population is as follows:
D=83.669L2-70.977L15-26.518F11+72.540F33+44.416F44+26.146；
For correcting the impact of Eriocheir sinensis body specification difference on morphological feature value, 5 measured morphological feature values, all divided by the wide value of body of its corresponding close crab, are carried out to discriminatory analysis using the eigen value of processing as standard value;
By after the female crab to be identified morphological feature value standardization corresponding with male crab, the female crab of substitution and male crab are differentiated in formula respectively: when D>0, be the Changjiang river Eriocheir sinensia wild population; During D<0, it is Eriocheir sinensis colony.
The present invention is based on 39 morphological data that the male and female crab of Eriocheir sinensia wild population and cultured population is measured, then carry out respectively progressively discriminatory analysis, all filter out 5 Main Morphology parameters and set up respectively Discrimination Functions.From discriminating situation, the average fit rate of the male crab of Liang Ge colony and female crab is respectively 92% and 94%, and identification result is better.Identification result to 50 the Changjiang river Eriocheir sinensias shows, the discriminating accuracy rate of the male crab of wild population is greater than the male crab of cultured population, and both average fit rates reach more than 92%.Illustrate that the present invention has reached the object of differentiating Eriocheir sinensia wild population and cultured population by discriminatory analysis progressively.
Claims (1)
- ParentEriocheir sinensis based on morphological parameters wild with cultured population discrimination method, employing is at the measurement point of the Eriocheir sinensia carapace back side and step place: interior volume tooth A or A ', outer volume tooth B or B ', eyeground C or C ', the first side tooth D or D ', E or E ' at the bottom of the second side tooth, F or F ' at the bottom of the 3rd side tooth, G or G ' at the bottom of the 4th side tooth, the wide outermost point HH ' of body, trailing edge circle angle I or I ', J in trailing edge, K in neck ditch, L incises in central authorities, AA ' on line middle part L ', step meropodium site M, N, step propodite site O, P, step dactylus site P, Q, and adopt following parameter-definition: L1-AA ', L2-BB ', L3-CC ', L4-DD ', L5-EE ', L6-FF ', L7-GG ', L8-II ', L9-JL ', L10-JL, L11-KL, L12-KJ, L13-IL, L14-CI, L15-EI, L16-FI, L17-GI, L18-HH ', F11, F21, F31, the MN of F41-1～4 step, F12, F22, F32, F42-the 1st OP to the 4th step, F13, F23, F33, propodite the widest part of F43-the 1st to the 4th step is wide, F14, F24, F34, F44-the 1st PQ to the 4th step, F15, F25, F35, dactylus the widest part of F45-the 1st to the 4th step is wide, the alphabetical L-carapace form variable comprising in parameter, F-step form variable, H-height form variable, it is characterized in that differentiating that operating procedure is as follows:Differentiate after close crab male and female sex, first adopt slide measure to choose measurement point at the Eriocheir sinensia carapace back side and 1st～4 steps place, every close crab is measured filter out 5 length data, male and female are carried out respectively record, the concrete measurement parameter of female crab is L2, L4, L6, F15, F44, and the concrete measurement parameter of male crab is L2, L15, F11, F33, F44; For female crab, 5 morphological parameters L2, the L4, L6, F15, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of female crab of setting up Eriocheir sinensia wild population and cultured population is as follows: D=66.613L2+31.203L4-43.969L6-159.018F15+34.628F44+2.278; For male crab, 5 morphological parameters L2, the L15, F11, F33, the F44 that utilize stepwise regression analysis to conclude, the discriminating formula of male crab of setting up Eriocheir sinensia wild population and cultured population is as follows: D=83.669L2-70.977L15-26.518F11+72.540F33+44.416F44+26.14 6; For correcting the impact of Eriocheir sinensis body specification difference on morphological feature value, by 5 measured morphological feature values all divided by the wide value of body of its corresponding close crab, using the eigen value of processing as standard value; By after the female crab to be identified morphological feature value standardization corresponding with male crab, the female crab of substitution and male crab are differentiated in formula respectively: when D>0, be Eriocheir sinensia wild population; During D<0, it is Eriocheir sinensis colony.
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