CN103125982A - Preparation method for fish brain microcapsules - Google Patents
Preparation method for fish brain microcapsules Download PDFInfo
- Publication number
- CN103125982A CN103125982A CN2012102539328A CN201210253932A CN103125982A CN 103125982 A CN103125982 A CN 103125982A CN 2012102539328 A CN2012102539328 A CN 2012102539328A CN 201210253932 A CN201210253932 A CN 201210253932A CN 103125982 A CN103125982 A CN 103125982A
- Authority
- CN
- China
- Prior art keywords
- fish
- fish brain
- brain
- microcapsules
- enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicinal Preparation (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a preparation method for fish brain microcapsules. The preparation method for the fish brain microcapsules includes the steps: taking fish brains and homogenizing, adding trypsin to carry out enzymolysis, adding pepsin to carry out the enzymolysis after deactivating enzyme so as to achieve enzymatic hydrolysate, adding emulgator and Vitamin E into the enzymatic hydrolysate and mixing to achieve fish brain emulsion, preparing wall material aqueous solution, adding the fish brain emulsion into the wall material aqueous solution to be through dispersed emulsion, homogenizing after sterilizing, and getting the fish brain microcapsules through spray drying. According to the preparation method for the fish brain microcapsules, waste fish heads of traditional fishery are adopted as raw materials for preparing products for brain fitness, so that economic benefit is improved. Complex enzyme is adopted to carry out the enzymolysis of the fish brains, protein in the fish brains is enabled to be translated into free amino acids and small peptides (< 3 KD) to a maximum degree, blood brain barrier is easy to penetrate, and the functions of central nervous system nutrition and central nervous system protection are good. Besides, microcapsules are adopted as a packaging mode, polyunsaturated fatty acids such as docosahexaenoic acid (DHA) contained in the fish brain can be saved stably for a long time, so that use of large doses of antioxidant and preservative can be avoided.
Description
Technical field
The present invention relates to the extracting method of fish brain material, in particular, relate to the method for utilizing the fish brain to extract brain-invigorating capsule.
Background technology
Since the 1950's, the world fisheries total output increases with the speed of 6%-7% always, although fishery production surpasses 100,000,000 tons already, has nearly 1/3 catches to fail directly to be utilized by the mankind.China's aquatic products total amount surpasses 5,200 ten thousand tons, becomes world fisheries and produces the first big country in continuous 20 years.The fish head is the main leftover bits and pieces in the fish process, has occupied the 15-30% left and right of fish gross weight.The fish head is rich in protein, fat, calcium, phosphorus, iron, vitamin, and the amino acid pattern of protein needs close to human body, is rich in unrighted acid and the phosphatide such as DHA, EPA in aliphatic acid, has certain value of exploiting and utilizing.
At present the exploitation of fish head mainly contained following approach: 1. directly aquatic foods freeze or freezing fish head, do the semi-finished product such as adult fish heads chafing dish, supply market.This method is used limited mainly for part fish head; 2. the fish head is dry, pulverizing, be ground into fishbone dust, is processed into animal feed.Although this method is simple, the added value of product of producing is lower; 3. reclaim protein (peptide) and fish oil from the fish head, develop Related product.This method Recent study is more, but related-art process is not yet ripe, does not really realize extensive commercial application.For utilizing level not high to the fish head at present, the series of problems such as processed goods is with low content of technology, added value is low is badly in need of seeking solution.
The fish capsules of brain generally acknowledges to have the brain-nourishing intelligence-benefiting effect.Unrighted acid and the phosphatide such as the DHA that the fish brain contains, EPA can promote the g and D of cranial nerve cell.In recent years, the neurotrophy of terrestrial animal Cerebrolysin Vial and neuroprotection are subject to extensive approval, and Related product-Cerebrolysin uses clinically as medicine.Be rich in protein in the fish brain, it be prepared into the brain nutrition peptide and amino acid can be removed the terrestrial animal property diseases such as rabid ox disease, swine flu to the potential threat of health.As seen, take the fish brain as development of raw materials all nutrition type health product, keep the effect whole good for health of fish brain, have a extensive future.But, there is no at present correlation technique and product and occur.This patent will be produced full brain composition soft capsule take the fish brain as raw material after its enzymolysis, both kept the health efficacy of the full brain of fish brain, and the form of soft capsule can guarantee the stability of polyunsaturated fatty acid, has widened the approach that utilizes of fish head.
Summary of the invention
The present invention is intended to produce a kind of brain tonic and intelligence development type product, and the waste fish head that has particularly adopted traditional fishery is that raw material prepares product for invigorating function of brain, reaches the purpose that promotes economic benefit.
In order to achieve the above object, the preparation method of a kind of fish brain of the present invention microcapsules comprises the steps:
Step1, get the fish brain, homogenate obtains homogenate;
Step2,300-800 watt of ultrasonic wave of use were processed described homogenate 10-30 minute; Accent pH is 6-9; Add trypsase, at 30-50 ℃ of lower enzymolysis; After enzymolysis 100 ℃ of enzymes that go out (preferred more than 10 minutes or 10 minutes);
Step3, to transfer pH again be 2-6; Add pepsin, 30-50 ℃ of enzymolysis obtains enzymolysis liquid, and transfers pH to neutral;
Step4, under 50-60 ℃, add the emulsifying agent of described enzymolysis liquid weight 0.1-0.5% in the described enzymolysis liquid; Add simultaneously the vitamin E of described enzymolysis liquid weight 0.005-0.015%, stir and evenly mix, obtain fish brain emulsion;
Step5,1:3-1:6 mixes starch octenyl succinate anhydride and glucose syrup in mass ratio, is dissolved in envelope-bulk to weight ratio 1-2 50-70 ℃ of water doubly, and adds beta-schardinger dextrin-to make its mass concentration reach 0.5-1.5%, and fully dissolving obtains the wall material aqueous solution;
Step6, described fish brain emulsion is joined volume ratio is in the 0.5-1.5 described wall material aqueous solution doubly, dispersion and emulsion; Then 100-120 ℃ sterilization 10-20 minute, under 3-5 MPa condition homogeneous 1-2 minute, obtain homogenizing fluid; The spray-dried fish brain hydrolysate microcapsules that get of described homogenizing fluid.
Under optimal way, described in step Step2, trypsase is selected EC3.4.21.4; Described in step Step3, pepsin is selected EC3.4.23.1.And described trypsase and described pepsic addition be the 0.1-2.0% of described homogenate weight, and enzymolysis time is 1-4 hour.In addition, regulate pH in step Step2 and Step3 and preferentially select citric acid or NaOH.
Under optimum way, described in step Step4, emulsifying agent is selected molecule distillating monoglyceride; The mode of dispersion and emulsion described in step Step6 was: with ten thousand rev/mins of dispersion and emulsion 1-2 of 1-2 minutes; Spray-dired EAT described in step Step6 is 160-190 ℃, and leaving air temp is 60-90 ℃.
The present invention compared with prior art the present invention has following advantage:
1, prepare first brain tonic and intelligence development type product take the fish brain as raw material;
2, adopt complex enzyme zymohydrolysis fish brain, to greatest extent protein transduction in the fish brain is turned to free amino acid and little peptide (＜3KD), easily see through blood-brain barrier, its maincenter neurotrophy, nervous centralis protective effect are stronger;
3, product adopts the packaged of microcapsules, and the polyunsaturated fatty acid that contains in the fish brain can be for a long time, the stable preservation, avoids using antioxidant and the anticorrisive agent of heavy dose.
The specific embodiment
Embodiment 1: get fresh fish head, open skull, take out the fish brain, homogenate.Homogenate was processed 10 minutes with 300 watts of ultrasonic waves; Transferring pH with 1 mol/L citric acid is 6; 0.1% the trypsase (EC3.4.21.4) that adds fish brain homogenate weight, 30 ℃ of enzymolysis 4 hours; 100 ℃ of enzymes 10 minutes of going out; Transferring pH with 1 mol/L citric acid is 2; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.1%, 30 ℃ of enzymolysis 4 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 50 ℃, adds molecule distillating monoglyceride, addition is 0.1% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.005% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3 mix, be dissolved in 60 ℃ of water of 1 times of volume (v/w), add beta-schardinger dextrin-to make its mass concentration reach 0.5%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 0.5 times of volume (v/v) prepares 10,000 rev/mins of high speed dispersion emulsification 1 minute, 100 ℃ of sterilizations 10 minutes, high-pressure homogeneous 1 minute of 3 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (160 ℃ of EATs, 60 ℃ of leaving air temps).
Embodiment 2: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 30 minutes with 800 watts of ultrasonic waves; Transferring pH with 3 mol/L NaOH is 9; 2.0% the trypsase (EC3.4.21.4) that adds fish brain homogenate weight, 50 ℃ of enzymolysis 1 hour; 100 ℃ of enzymes 15 minutes of going out; Transferring pH with 3 mol/L citric acids is 3; Add pepsin (EC3.4.23.1), the enzyme dosage is 2.0%, 50 ℃ of enzymolysis 1 hour of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 70 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.5% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.015% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:6 mix, be dissolved in 60 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.5%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.5 times of volumes (v/v) prepare 20,000 rev/mins of high speed dispersion emulsification 2 minutes, 120 ℃ of sterilizations 20 minutes, high-pressure homogeneous 2 minutes of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (190 ℃ of EATs, 90 ℃ of leaving air temps).
Embodiment 3: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 25 minutes with 400 watts of ultrasonic waves; Transferring pH with 1 mol/L NaOH is 8; 1.0% the trypsase that adds fish brain homogenate weight, 40 ℃ of enzymolysis 3 hours; 100 ℃ of enzymes 10 minutes of going out; Transferring pH with 1 mol/L citric acid is 4; Add pepsin (EC3.4.23.1), the enzyme dosage is 1.0%, 40 ℃ of enzymolysis 3 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 60 ℃, adds molecule distillating monoglyceride, addition is 0.2% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.006% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:4 mix, be dissolved in 60 ℃ of water of 1 times of volume (v/w), add beta-schardinger dextrin-to make its mass concentration reach 0.6%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 0.7 times of volume (v/v) prepares 1.5 ten thousand rev/mins of high speed dispersion emulsification 1.5 minutes, 110 ℃ of sterilizations 12 minutes, high-pressure homogeneous 1 minute of 4 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (165 ℃ of EATs, 65 ℃ of leaving air temps).
Embodiment 4: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 20 minutes with 500 watts of ultrasonic waves; Transferring pH with 1 mol/L NaOH is 9; 0.5% the trypsase that adds fish brain homogenate weight, 40 ℃ of enzymolysis 4 hours; 100 ℃ of enzymes 10 minutes of going out; Transferring pH with 1 mol/L citric acid is 6; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.5%, 40 ℃ of enzymolysis 4 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 55 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.3% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.007% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:5 mix, be dissolved in 55 ℃ of water of 1 times of volume (v/w), add beta-schardinger dextrin-to make its mass concentration reach 0.7%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 0.8 times of volume (v/v) prepares 10,000 rev/mins of high speed dispersion emulsification 1 minute, 105 ℃ of sterilizations 15 minutes, high-pressure homogeneous 1.5 minutes of 3 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (170 ℃ of EATs, 70 ℃ of leaving air temps).
Embodiment 5: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 15 minutes with 600 watts of ultrasonic waves; Transferring pH with 2 mol/L citric acids is 7; 0.5% the trypsase that adds fish brain homogenate weight, 30 ℃ of enzymolysis 3 hours; 100 ℃ of enzymes 10 minutes of going out; Transferring pH with 2 mol/L citric acids is 2.5; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.5%, 30 ℃ of enzymolysis 3 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 60 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.4% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.008% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:5 mix, be dissolved in 60 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 0.8%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 0.9 times of volume (v/v) prepares 20,000 rev/mins of high speed dispersion emulsification 2 minutes, 110 ℃ of sterilizations 15 minutes, high-pressure homogeneous 2 minutes of 45bar; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (175 ℃ of EATs, 75 ℃ of leaving air temps).
Embodiment 6: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 20 minutes with 700 watts of ultrasonic waves; Transferring pH with 1 mol/L citric acid is 6; Add trypsase (EC3.4.21.4), the enzyme dosage is 0.4%, 35 ℃ of enzymolysis 1 hour of fish brain homogenate weight; 100 ℃ of enzymes 15 minutes of going out; Transferring pH with 2 mol/L citric acids is 3; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.1%, 35 ℃ of enzymolysis 3 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 50-60 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.5% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.009% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3-1:6 mix, be dissolved in 60 ℃ of water of 1-2 times of volume (v/w), add beta-schardinger dextrin-to make its mass concentration reach 0.9%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.0 times of volumes (v/v) prepare 10,000 rev/mins of high speed dispersion emulsification 2 minutes, 100 ℃ of sterilizations 20 minutes, high-pressure homogeneous 1 minute of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (180 ℃ of EATs, 80 ℃ of leaving air temps).
Embodiment 7: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 28 minutes with 350 watts of ultrasonic waves; Transferring pH with 1 mol/L NaOH is 8; Add trypsase (EC3.4.21.4), the enzyme dosage is 1.0%, 35 ℃ of enzymolysis 2 hours of fish brain homogenate weight; 100 ℃ of enzymes 10 minutes of going out; Transferring pH with 1 mol/L citric acid is 3.5; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.5%, 40 ℃ of enzymolysis 1 hour of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 50 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.3% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.01% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3 mix, be dissolved in 65 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.0%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.2 times of volumes (v/v) prepare 20,000 rev/mins of high speed dispersion emulsification 2 minutes, 110 ℃ of sterilizations 15 minutes, high-pressure homogeneous 1 minute of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (185 ℃ of EATs, 85 ℃ of leaving air temps).
Embodiment 8: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 20 minutes with 450 watts of ultrasonic waves; Transferring pH with 1 mol/L NaOH is 8.5; Add trypsase (EC3.4.21.4), the enzyme dosage is 0.4%, 35 ℃ of enzymolysis 2 hours of fish brain homogenate weight; 100 ℃ of enzymes 11 minutes of going out; Transferring pH with 1 mol/L citric acid is 4; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.4%, 35 ℃ of enzymolysis 4 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 55 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.4% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.011% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:4 mix, be dissolved in 60 ℃ of water of 1 times of volume (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.0%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.1 times of volumes (v/v) prepare 10,000 rev/mins of high speed dispersion emulsification 1-2 minute, 100 ℃ of sterilizations 20 minutes, the 5 high-pressure homogeneous 1-2 of MPa minutes; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (180 ℃ of EATs, 90 ℃ of leaving air temps).
Embodiment 9: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 15 minutes with 550 watts of ultrasonic waves; Transferring pH with 3 mol/L NaOH is 8; Add trypsase (EC3.4.21.4), the enzyme dosage is 1.2%, 45 ℃ of enzymolysis 3 hours of fish brain homogenate weight; 100 ℃ of enzymes 12 minutes of going out; Transferring pH with 3 mol/L citric acids is 4.5; Add pepsin (EC3.4.23.1), the enzyme dosage is 1.1%, 45 ℃ of enzymolysis 4 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 60 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.1% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.012% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3 mix, be dissolved in 55 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.3%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.3 times of volumes (v/v) prepare 20,000 rev/mins of high speed dispersion emulsification 2 minutes, 120 ℃ of sterilizations 10 minutes, high-pressure homogeneous 2 minutes of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (160 ℃ of EATs, 75 ℃ of leaving air temps).
Embodiment 10: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 22 minutes with 650 watts of ultrasonic waves; Transferring pH with 1 mol/L citric acid is 6; Add trypsase (EC3.4.21.4), the enzyme dosage is 0.5%, 35 ℃ of enzymolysis 3.5 hours of fish brain homogenate weight; 100 ℃ of enzymes 13 minutes of going out; Transferring pH with 1 mol/L citric acid is 3; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.25%, 35 ℃ of enzymolysis 3 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 50 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.5% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.013% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3 mix, be dissolved in 65 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.4%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.4 times of volumes (v/v) prepare 20,000 rev/mins of high speed dispersion emulsification 2 minutes, 105 ℃ of sterilizations 15 minutes, high-pressure homogeneous 2 minutes of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (180 ℃ of EATs, 70 ℃ of leaving air temps).
Embodiment 11: get the fish head of fresh (or freezing preservation, flowing water thaws), open skull, take out the fish brain, homogenate.Homogenate was processed 12 minutes with 750 watts of ultrasonic waves; Transferring pH with 1 mol/L NaOH is 8; Add trypsase (EC3.4.21.4), the enzyme dosage is 0.1%, 40 ℃ of enzymolysis 2 hours of fish brain homogenate weight; 100 ℃ of enzymes 15 minutes of going out; Transferring pH with 1 mol/L citric acid is 2.5; Add pepsin (EC3.4.23.1), the enzyme dosage is 0.45%, 45 ℃ of enzymolysis 3.5 hours of fish brain homogenate weight, and transfers pH to neutral.
Fish brain enzymolysis liquid is heated to 60 ℃, adds emulsifying agent (molecule distillating monoglyceride), addition is 0.1% of fish brain enzymolysis liquid weight, adds vitamin E, and addition is 0.014% of fish brain enzymolysis liquid weight, stirs and evenly mixs, and obtains fish brain emulsion.Separately with starch octenyl succinate anhydride and glucose syrup in mass ratio 1:3 mix, be dissolved in 60 ℃ of water of 2 times of volumes (v/w), add beta-schardinger dextrin-to make its mass concentration reach 1.1%, fully dissolving, obtain the wall material aqueous solution.Fish brain emulsion is slowly joined in the wall material aqueous solution that 1.5 times of volumes (v/v) prepare 20,000 rev/mins of high speed dispersion emulsification 1 minute, 100 ℃ of sterilizations 20 minutes, high-pressure homogeneous 1 minute of 5 MPas; Namely get fish brain hydrolysate microcapsules after homogenizing fluid spray-dried (160 ℃ of EATs, 90 ℃ of leaving air temps).
The above; only be the better specific embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (6)
1. the preparation method of fish brain microcapsules, is characterized in that, comprises the steps:
S1, get the fish brain, homogenate obtains homogenate;
S2,300-800 watt of ultrasonic wave of use were processed described homogenate 10-30 minute; Accent pH is 6-9; Add trypsase, at 30-50 ℃ of lower enzymolysis; After enzymolysis at 100 ℃ of enzymes that go out;
S3, to transfer pH again be 2-6; Add pepsin, 30-50 ℃ of enzymolysis obtains enzymolysis liquid, and transfers pH to neutral;
S4, under 50-60 ℃, add the emulsifying agent of described enzymolysis liquid weight 0.1-0.5% in the described enzymolysis liquid; Add simultaneously the vitamin E of described enzymolysis liquid weight 0.005-0.015%, stir and evenly mix, obtain fish brain emulsion;
S5,1:3-1:6 mixes starch octenyl succinate anhydride and glucose syrup in mass ratio, is dissolved in envelope-bulk to weight ratio 1-2 50-70 ℃ of water doubly, and adds beta-schardinger dextrin-to make its mass concentration reach 0.5-1.5%, and fully dissolving obtains the wall material aqueous solution;
S6, described fish brain emulsion is joined volume ratio is in the 0.5-1.5 described wall material aqueous solution doubly, dispersion and emulsion; Then 100-120 ℃ sterilization 10-20 minute, under 3-5 MPa condition homogeneous 1-2 minute, obtain homogenizing fluid; The spray-dried fish brain hydrolysate microcapsules that get of described homogenizing fluid.
2. the preparation method of fish brain microcapsules according to claim 1, is characterized in that,
Described in described step S2, trypsase is selected EC3.4.21.4; Described in step S3, pepsin is selected EC3.4.23.1;
Described trypsase and described pepsic addition are the 0.1-2.0% of described homogenate weight, and enzymolysis time is 1-4 hour.
3. the preparation method of fish brain microcapsules according to claim 1, is characterized in that, regulates pH in described step S2 and S3 and select citric acid or NaOH.
4. the preparation method of according to claim 1-3 arbitrary described fish brain microcapsules, is characterized in that,
Described in step S4, emulsifying agent is selected molecule distillating monoglyceride.
5. the preparation method of fish brain microcapsules according to claim 4, is characterized in that, the mode of dispersion and emulsion described in step S6 was: with ten thousand rev/mins of dispersion and emulsion 1-2 of 1-2 minutes.
6. the preparation method of fish brain microcapsules according to claim 5, is characterized in that, spray-dired EAT described in step S6 is 160-190 ℃, and leaving air temp is 60-90 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210253932.8A CN103125982B (en) | 2012-03-31 | 2012-07-20 | Preparation method for fish brain microcapsules |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210092088 | 2012-03-31 | ||
| CN201210092088.5 | 2012-03-31 | ||
| CN201210253932.8A CN103125982B (en) | 2012-03-31 | 2012-07-20 | Preparation method for fish brain microcapsules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103125982A true CN103125982A (en) | 2013-06-05 |
| CN103125982B CN103125982B (en) | 2014-10-15 |
Family
ID=48486928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210253932.8A Active CN103125982B (en) | 2012-03-31 | 2012-07-20 | Preparation method for fish brain microcapsules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103125982B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912760A (en) * | 2017-11-17 | 2018-04-17 | 黑龙江康普生物科技有限公司 | A kind of composite functional male silk moth powder and its application |
| CN114452259A (en) * | 2021-07-28 | 2022-05-10 | 安徽旺盛添加剂有限公司 | Vitamin D micro-capsule calcium tablet and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478423A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Abalone food and its preparation method |
| CN1480158A (en) * | 2002-09-08 | 2004-03-10 | 姜雨田 | Fish brain oil and its extraction method |
| CN1559443A (en) * | 2004-03-01 | 2005-01-05 | 兰泽桥 | Sturgeon chondroition capsule and instant granula prepn. method therefor |
| CN101785550A (en) * | 2010-01-29 | 2010-07-28 | 陈敏新 | Fish brain sauce condiment and processing technique thereof |
| CN101844061A (en) * | 2009-03-25 | 2010-09-29 | 中国科学院大连化学物理研究所 | Method for preparing microcapsules using starch sodium octenyl succinate(SSOS) as wall material |
| CN101940240A (en) * | 2010-07-01 | 2011-01-12 | 大连工业大学 | Method for preparing fish oil ethyl ester microcapsule from fish pomace |
-
2012
- 2012-07-20 CN CN201210253932.8A patent/CN103125982B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478423A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Abalone food and its preparation method |
| CN1480158A (en) * | 2002-09-08 | 2004-03-10 | 姜雨田 | Fish brain oil and its extraction method |
| CN1559443A (en) * | 2004-03-01 | 2005-01-05 | 兰泽桥 | Sturgeon chondroition capsule and instant granula prepn. method therefor |
| CN101844061A (en) * | 2009-03-25 | 2010-09-29 | 中国科学院大连化学物理研究所 | Method for preparing microcapsules using starch sodium octenyl succinate(SSOS) as wall material |
| CN101785550A (en) * | 2010-01-29 | 2010-07-28 | 陈敏新 | Fish brain sauce condiment and processing technique thereof |
| CN101940240A (en) * | 2010-07-01 | 2011-01-12 | 大连工业大学 | Method for preparing fish oil ethyl ester microcapsule from fish pomace |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912760A (en) * | 2017-11-17 | 2018-04-17 | 黑龙江康普生物科技有限公司 | A kind of composite functional male silk moth powder and its application |
| CN114452259A (en) * | 2021-07-28 | 2022-05-10 | 安徽旺盛添加剂有限公司 | Vitamin D micro-capsule calcium tablet and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103125982B (en) | 2014-10-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101840213B1 (en) | Production method of low molecular collagen peptide and food composition using this low molecular collagen peptide | |
| KR101861333B1 (en) | Nutrition jelly for pets and manufacturing method of the same | |
| CN102366026A (en) | Method for making pet food attractant | |
| CN104068418B (en) | A kind of sea cucumber drink production method that strengthens immunity | |
| CN102578363B (en) | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage | |
| CN103859368A (en) | Sea cucumber beverage and production method thereof | |
| CN104738650A (en) | Hydrolytic protein peptide product | |
| CN104605025A (en) | Pecan-milk compound protein beverage and processing method thereof | |
| CN109007517A (en) | A kind of astaxanthin Seabuckthorn Oil solid beverage and preparation method thereof | |
| CN103125982B (en) | Preparation method for fish brain microcapsules | |
| CN101467682A (en) | Shrimp oil health-care product and preparation method thereof | |
| CN105341948A (en) | Preparation method of oyster peptides | |
| CN108850758A (en) | A kind of preparation method of the fructus lycii enzyme beverage with anti-fatigue effect | |
| CN103125989B (en) | Method of extracting fish cerebrol and fish brain peptide | |
| CN106031436A (en) | Nutritional preparation containing egg white and preparation method thereof | |
| CN107048414A (en) | A kind of composite protein powder rich in peanut bioactive peptides and preparation method thereof | |
| CN106579461A (en) | Collagen protein oral liquid and preparation method thereof | |
| CN103976205A (en) | Jelly for improving internal secretion and preparation method of jelly | |
| CN106387896A (en) | Chinese yam medlar walnut polypeptide chewable tablet for nourishing spleen and kidney and preparation method thereof | |
| CN107417365A (en) | A kind of mushroom culture medium and preparation method thereof | |
| CN106036394A (en) | Methods for producing starch selenium polysaccharide and selenium-rich pre-gelatinized nutritional rice paste by using pulsed electric fields | |
| CN104705660A (en) | Special protein powder for children | |
| CN108740664A (en) | A kind of soybean peptide solid beverage and its preparation process | |
| CN103540512A (en) | Crocodile liver healthcare wine and preparation method thereof | |
| CN106360220A (en) | Black fungus-malt healthcare drink and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |