CN103114077A - Process for extracting active SOD (Superoxide Dismutase) from wheat seedlings - Google Patents

Abstract

The invention discloses a process for extracting active SOD (Superoxide Dismutase) from wheat seedlings. The process comprises the following steps of: harvesting fresh wheat seedlings, sorting, cleaning, juicing, filtering, concentrating, spraying and drying, collecting powder, checking and accepting, and packaging and storing. According to the process disclosed by the invention, the time from harvesting the fresh wheat seedlings to collecting the powder is controlled within 5 hours. The whole process is a physical process, the mechanical degree is high, the extracting rate is high and the temperature is controlled to 25 DEG C to 45 DEG C. The preparation process disclosed by the invention can be used for efficiently extracting SOD in the wheat seedlings and sufficiently storing and keeping the function activities; besides, the process is green and environment-friendly, the wheat seedlings can be recycled and the residues can be used as feed, so that industrial pollution is avoided.

Description

A kind of technique of extracting active SOD from wheat seeding

Technical field

The invention belongs to active substance of plant extractive technique field, more specifically say a kind of technique of extracting active SOD from wheat seeding.

Background technology

Superoxide-dismutase (english abbreviation SOD) have human body waste " street cleaner ", " anti-ageing king ", " beauty treatment favourite son's " title, being a kind of important active substances of promoting HUMAN HEALTH as free-radical scavengers, is also the important functional food ingredient of a class.As far back as the middle and later periods sixties, just have scientist to extract successively SOD from animal blood, but the common defects that these SOD exist is that price is high, security is unstable.Therefore, the WHO of the World Health Organization appeals not extract SOD from animal plasma, and European Union has also announced progressively to stop using animal proteinum, advocates and uses vegetable-protein.

In recent years, the production technology of vegetable-protein has obtained breakthrough, but the overwhelming majority is the chemical solvents extraction process, and these class methods exist SOD dangerous by the sex change of chemical pollution, generally can not make highly purified active plant SOD, and the chemical solvents waste liquid can cause a hidden trouble to environment.In addition, the extraction method that some are novel, as: microwave-assisted extraction, ultrasonic extraction etc. also mostly rest on laboratory stage.Be the growing demand of satisfying the market to vegetable active SOD, seek widely material of vegetable origin, realize that the large-scale industrialization production of highly effective and safe is the only way which must be passed, enterprise need give full play to own technical characterstic and advantage, study, reference, absorption modern technique and experience, extractive technique and the technique of the plant SOD that constantly brings forth new ideas.

CN95117689.7 discloses a kind of preparation technology of wheat seedling powder, and the product of production is mainly used in nutrient.SOD surpasses 45 ℃ with inactivation to temperature sensitive, and this technical process is undesirable to producing temperature and time control, and production efficiency is still waiting to improve.The SOD number of plant is limited owing to containing, and in wheat seeding, the content of SOD far away higher than other plant, therefore, must take full advantage of resource, and the work meaning of the active SOD of high efficiency extraction is far-reaching from wheat seeding under reasonable terms.

Summary of the invention

Technical problem to be solved by this invention is, overcomes deficiency of the prior art, provide that a kind of raw material is easy to get, safe and efficient, environmental protection extract the preparation technology of active SOD from wheat seeding.

A kind of technique of extracting active SOD from wheat seeding, this technical process comprises the following steps:

(1) raw material harvesting, sorting: at harvesting of best harvest time of wheat seeding (winter) wheat seeding, Lao Ye, Huang Ye are removed in sorting, obtain fresh and tender part as the target raw material, and every batch of raw material harvesting, sorting were controlled in 0.5 hour used time;

(2) clean, broken, squeeze the juice: the target raw material that step (1) is obtained drops into the automatic turning cleaning equipment, remove surface impurity through rinsing, spray, fresh wheat seeding after cleaning enters crusher to carry out physical wall breaking and squeezes the juice, and the crusher rotating speed is 1440r/min; Material after fragmentation is pressed juice by juice extractor; Control in 1 hour used time;

(3) filter, concentrate: the pasty state wheat juice that step (2) is made filters through resonance filter, adopt single-effect vacuum cryoconcentration method with the filtrate continuous concentration, control vacuum tightness and be-0.090MPa extremely-0.095MPa, vaporization temperature is 40 ℃ to 45 ℃, obtain active substance content and be 15% to 20% concentrated solution, solution remains pH value 7.4～7.8; This step was controlled in 1.5 hours used times;

(4) spraying drying, receipts powder: the concentrated solution that employing moment spray-drying process makes step (3) carries out wink-dry and granulates, the spray-drying tower height that adopts is 10.3m, per hour process the 300L concentrated solution, keeping the interior temperature of tower is 25 ℃ to 45 ℃, the moment charging is completely dried in 10 to 20 seconds, in time receive powder by cyclonic separator, between the receipts powder, relative humidity is 35% to 40%, and temperature remains on 15 ℃ to 25 ℃; This step operation was controlled in 1.5 hours used times;

(5) check and accept, pack warehouse-in: with the powder barrelling sealing that step (4) makes, warehouse-in is preserved.

Further, in step (2), the production plant envrionment temperature is natural room temperature 5-40 ℃.

In step (4), by controlling inlet temperature and air outlet temperature, can make the moisture evaporation of concentrated solution in spray-drying tower reach balance with external heat.

In step (4), before spray-drying process, add appropriate maltodextrin in dish juice concentrated solution, stir, can prevent powder sticking wall in spray-drying tower.

The present invention compared with prior art has the following advantages and effect: 1, the strict temperature control of whole process, in production process, each link is all controlled temperature under the state of the suitable existence of SOD: 25 ℃ to 45 ℃, greatly improved the extraction survival rate of SOD.

2, technique is simple, easy to operate, and the whole process level of automation is high, short, high efficiency of time, and extraction time shortens more than 4/5 greatly than traditional method (1-2 days), and the used time was less than 5 hours, and is workable, remarkable in economical benefits.

3, the present invention only relates to physical process, comprises fragmentation, squeezes the juice, cryoconcentration, spraying drying, and whole process can effectively be preserved active components of plants without chemical reaction, and SOD purity is high, and every 100 grams contain: superoxide-dismutase (SOD) 〉=2000u.

4, the wheat seeding dregs can be used as feed and agricultural fertilizer, green, environmental protection, capable of circulation.

Active SOD extraction process of the present invention has green, environmental protection, feature capable of circulation simultaneously concurrently, zero discharging of waste liquid in production process, and also the wheat seeding dregs can be used as poultry, cattle food and agricultural organic fertilizer.Therefore, the present invention has good economic and social benefit.

Embodiment

Now in conjunction with the embodiments the present invention is described in further detail

Embodiment 1

(1) in wheat seeding best harvest time, hand harvest's wheat seeding is removed Lao Ye, Huang Ye through sorting, obtains fresh and tender wheat seeding 2000kg, 30 minutes used times.

(2) fresh and tender wheat seeding is evenly dropped into the automatic turning cleaning equipment, remove surface impurity through rinsing, spray, fresh wheat seeding after cleaning enters cage mill through travelling belt to carry out physical wall breaking and squeezes the juice, and the crusher rotating speed is 1440r/min, blade indention, two-sided blade; The mixed solution of obtaining after fragmentation is pressed juice by the 5T/H type juice extractor with automatic deviation correction and automatic washing band function, gets 50 minutes pasty state wheat juice used times.

(3) pasty state wheat juice is filtered to get clear liquid through resonance filter, adopt single-effect vacuum cryoconcentration method with the clear liquid continuous concentration, get the deep green concentrated solution, the vacuum tightness of vaporizer is-0.090MPa is to-0.095MPa, by effective cooling water system, the vaporization temperature that remains solution in vaporizer is 40 ℃ to 45 ℃, and gained concentrated solution pH7.8 is alkalescence.1.5 hours this step used times.

(4) concentrated solution employing moment spray-drying process is carried out the wink-dry granulation, the spray-drying tower height is 10.3m, per hour can process the 300L concentrated solution, keeping the interior temperature of tower is 25 ℃ to 45 ℃, and the moment charging is completely dried in 10 to 20 seconds, in time receives powder by cyclonic separator, sweep tower once in this batch of material production pedestrian's work that finishes to advance, sweep 10 minutes tower used times, between the receipts powder, relative humidity is 38%, 20 ℃ of temperature; Dry when receiving powder and sharing 1.5 hours.

(5) dry powder is weighed: 60kg, extraction yield is 3%, active SOD detected result: it is 2100u that every 100 gram dry powder contain superoxide-dismutase (SOD), and meeting the requirements, (every 100 grams contain: superoxide-dismutase (SOD) 〉=2000u), barrelling sealing warehouse-in is preserved.

Claims (3)

1. a technique of extracting active SOD from wheat seeding, is characterized in that, this technique comprises the following steps:

(1) raw material harvesting, sorting: at the best harvest time harvesting of wheat seeding wheat seeding, Lao Ye, Huang Ye are removed in sorting, obtain fresh and tender part as the target raw material, and every batch of raw material harvesting, sorting were controlled in 0.5 hour used time;

(2) clean, broken, squeeze the juice: the target raw material that step (1) is obtained drops into the automatic turning cleaning equipment, remove surface impurity through rinsing, spray, fresh wheat seeding after cleaning enters crusher to carry out physical wall breaking and squeezes the juice, and the crusher rotating speed is 1440r/min; Material after fragmentation is pressed juice by juice extractor; Control in 1 hour used time;

(3) filter, concentrate: the pasty state wheat juice that step (2) is made filters through resonance filter, adopt single-effect vacuum cryoconcentration method with the filtrate continuous concentration, control vacuum tightness and be-0.090MPa extremely-0.095MPa, vaporization temperature is 40 ℃ to 45 ℃, obtain solid matter content and be 15% to 20% concentrated solution, solution remains pH value 7.4～7.8; This step was controlled in 1.5 hours used times;

(4) spraying drying, receipts powder: the concentrated solution that employing moment spray-drying process makes step (3) carries out wink-dry and granulates, the spray-drying tower height that adopts is 10.3m, per hour process the 300L concentrated solution, keeping the interior temperature of tower is 25 ℃ to 45 ℃, the moment charging is completely dried in 10 to 20 seconds, in time receive powder by cyclonic separator, between the receipts powder, relative humidity is 35% to 40%, and temperature remains on 15 ℃ to 25 ℃; This step operation was controlled in 1.5 hours used times;

(5) check and accept, pack warehouse-in: with the powder barrelling sealing that step (4) makes, warehouse-in is preserved.

2. technique according to claim 1, is characterized in that, in step (2), the production plant envrionment temperature is natural room temperature 5-40 ℃.

3. technique according to claim 1, is characterized in that, in step (4), before spray-drying process, adds appropriate maltodextrin in concentrated solution, stirs.